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| 1 | The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3显示文摘增加的庄稼收益被要求支持世界范围的快速的人口生长。谷物重量是米饭产量的一个关键部件,但是控制它的内在的分子的机制留下逃犯。这里,我们报导为米饭谷物长度,重量和产量的控制克隆和一个新量的特点地点(QTL ) 的描述。这个地点, GL3.1,编码蛋白质磷酸酶 kelch (PPKL ) 家庭 —Ser/Thr 磷酸酶。GL3.1 是大谷物 WY3 变化的一个成员,它比小谷物 FAZ1 变化与更弱的 dephosphorylation 活动被联系。GL3.1-WY3 在小穗状花小穗影响蛋白质 phosphorylation 加速房间分割,从而导致更长的谷物和更高的收益。进一步的研究直接显示出那 GL3.1 dephosphorylates 它的底层, Cyclin-T1; 3,它很少仅仅在植物被学习了。Cyclin-T1 的 downregulation; 3 在更短的谷物导致了米饭,它在房间周期规定为 Cyclin-T 显示新奇功能。我们的调查结果为通过影响 Cyclin-T1 的 phosphorylation 的新奇调停磷酸酶的进程被驾驶的谷物尺寸和产量的规定建议新机制; 3 在房间周期前进期间,并且这样提供新卓见进位于庄稼种子开发下面的机制。我们引起了包含表明了增加的谷物产量的自然 GL3.1 等位基因的一个新变化,它显示 GL3.1 是为引起产量很高的庄稼的一个强大的工具。 | Peng Qi You-Shun Lin Xian-Jun Song Jin-Bo Shen Wei Huang Jun-Xiang Shan Mei-Zhen Zhu Liwen Jiang Ji-Ping Gao Hong-Xuan Lin | 2012 | Cell Research2012，22，12： | 132 |
| 2 | aunalysis of the Genome Sequence of the Medicinal Plant Salvia miltiorrhiza显示文摘 | Haibin Xu Jingyuan Song Hongmei Luo Yujun Zhang Qiushi Li Yingjie Zhu Jiang Xu Ying Li Chi Song Bo Wang Wei Sun Guoan Shen Xin Zhang Jun Qian Aijia Ji Zhichao Xu Xiang Luo Liu He Chuyuan Li Chao Sun Haixia Yah Guanghong Cui Xiwen Li Xian 'en Li Jianhe Wei Juyan Liu Yitao Wang Alice Hayward David Nelson Zemin Ning Reuben J. Peters Xiaoquan Qi Shilin Chen | 2016 | Molecular Plant2016，9，6： | 70 |
| 3 | Clinical characteristics of 24 asymptomatic infections with COVID-19 screened among close contacts in Nanjing,China显示文摘Previous studies have showed clinical characteristics of patients with the 2019 novel coronavirus disease(COVID-19)and the evidence of person-to-person transmission.Limited data are available for asymptomatic infections.This study aims to present the clinical characteristics of 24 cases with asymptomatic infection screened from close contacts and to show the transmission potential of asymptomatic COVID-19 virus carriers.Epidemiological investigations were conducted among all close contacts of COVID-19 patients(or suspected patients)in Nanjing,Jiangsu Province,China,from Jan 28 to Feb 9,2020,both in clinic and in community.Asymptomatic carriers were laboratory-confirmed positive for the COVID-19 virus by testing the nucleic acid of the pharyngeal swab samples.Their clinical records,laboratory assessments,and chest CT scans were reviewed.As a result,none of the 24 asymptomatic cases presented any obvious symptoms while nucleic acid screening.Five cases(20.8%)developed symptoms(fever,cough,fatigue,etc.)during hospitalization.Twelve(50.0%)cases showed typical CT images of ground-glass chest and 5(20.8%)presented stripe shadowing in the lungs.The remaining 7(29.2%)cases showed normal CT image and had no symptoms during hospitalization.These 7 cases were younger(median age:14.0 years;P=0.012)than the rest.None of the 24 cases developed severe COVID-19 pneumonia or died.The median communicable period,defined as the interval from the first day of positive nucleic acid tests to the first day of continuous negative tests,was 9.5 days(up to 21 days among the 24 asymptomatic cases).Through epidemiological investigation,we observed a typical asymptomatic transmission to the cohabiting family members,which even caused severe COVID-19 pneumonia.Overall,the asymptomatic carriers identified from close contacts were prone to be mildly ill during hospitalization.However,the communicable period could be up to three weeks and the communicated patients could develop severe illness.These results highlighted the importance of close contact tracing and longitudinally surveillance via virus nucleic acid tests.Further isolation recommendation and continuous nucleic acid tests may also be recommended to the patients discharged. | Zhiliang Hu Ci Song Chuanjun Xu Guangfu Jin Yaling Chen Xin Xu Hongxia Ma Wei Chen Yuan Lin Yishan Zheng Jianming Wang Zhibin Hu Yongxiang Yi Hongbing Shen | 2020 | Science China(Life Sciences)2020，63，5： | 77 |
| 4 | cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions显示文摘癌症房间被知道经历新陈代谢的 reprogramming 然而,支撑幸存和快速的增长 oncogenic 损害怎么在各种各样的强调条件下面协调新陈代谢的开关,尚待充分被阐明。这里,我们显示出葡萄糖或夫酸安的那剥夺,为癌症房间的二主要营养来源,被伴随由的戏剧性地激活的丝氨酸生合成小径(SSP ) 提高了 cMyc 表示。我们进一步鉴别那 cMyc 刺激了 SSP 激活由 transcriptionally 多重 SSP 酶的 upregulating 表示。而且,我们证明 cMyc 便于的 SSP 激活导致了提高的谷胱甘肽(GSH ) 生产,房间周期前进和 nucleic 酸合成,它在剥夺营养素的条件下面特别为房间幸存和增长是必要的。我们进一步揭开了那 phosphoserine 磷酸酶( PSPH ), SSP 小径的最后的限制率的酶,在 vitro 并且在 vivo 为 驾驶cMyc 的癌症前进是批评的,并且重要地, PSPH 的异常表示高度在 hepatocellular 与死亡被相关癌( HCC )病人,一般来说建议在这 调整cMyc 的酶,或 SSP 激活之间的一种潜在的原因的关系,并且癌症开发。一起拿,我们的结果表明 cMyc 的那异常表情导致提高的 SSP 激活,新陈代谢的开关的必要部分,在剥夺营养素的条件下面便于癌症前进。 | Linchong Sun Libing Song Qianfen Wan Gongwei Wu Xinghua Li Yinghui Wang Jin Wang Zhaoji Liu Xiuying Zhong Xiaoping He Shengqi Shen Xin Pan Ailing Li Yulan Wang Ping Gao Huiru Tang Huafeng Zhang | 2015 | Cell Research2015，25，4： | 33 |
| 5 | Shortened Basal Internodes Encodes a Gibberellin 2-Oxidase and Contributes to Lodging Resistance in Rice显示文摘改进住宿抵抗的繁殖半矮子变化被证明了在自从绿 revolution.?However 的来临,增加谷物产量极其成功,在亚洲繁殖半矮子米饭变化的多数主要依赖于 SD1 的变异的等位基因的基因介绍,它编码赤霉素( GA ) 20-oxidase , OsGA20ox2 ,为催化 GA 生合成。这里,我们报导新米饭住宿抵抗基因,弄短的基础 Internodes (SBI ) ,它编码赤霉素 ? 2-oxidase 并且明确地控制灰煤杆的延伸通过撤销的基础 internodes GA 活动。SBI 主要在灰煤杆被表示基础 internodes。基因分析显示 SBI 是影响米饭高度和住宿抵抗的半主导的基因。SBI 突变而产生之遗传的变体显示不同活动并且与米饭变化的高度被联系。与 SBI 等位基因的更高的活动繁殖与改进住宿抵抗和增加的产量产生新米饭变化。SBI 的发现为生产半矮子米饭显型提供一个理想的基因资源并且为与提高的住宿抵抗和高产量引起米饭变化提供有效策略。 | Chang Liu Jinshan Gui Chenjian Fu Hasi Yu Dongliang Song Junhui Shen Peng Qin Xuanming Liu Bin Han Yuanzhu Yang Laigeng Li | 2018 | Molecular Plant2018，11，2： | 33 |
| 6 | Bone-marrow mesenchymal stem cells reduce rat intestinal ischemia-reperfusion injury, ZO-1 downregulation and tight junction disruption via a TNF-α-regulated mechanism显示文摘AIM: To investigate the effect of bone-marrow mesenchymal stem cells (BM MSCs) on the intestinal mucosa barrier in ischemia/reperfusion (I/R) injury. METHODS: BM MSCs were isolated from male Sprague-Dawley rats by density gradient centrifugation, cultured, and analyzed by flow cytometry. I/R injury was induced by occlusion of the superior mesenteric artery for 30 min. Rats were treated with saline, BM MSCs (via intramucosal injection) or tumor necrosis factor (TNF)-α blocking antibodies (via the tail vein). I/R injury was assessed using transmission electron microscopy, hematoxylin and eosin (HE) staining, immunohistochemistry, western blotting and enzyme linked immunosorbent assay.RESULTS: Intestinal permeability increased, tight junctions (TJs) were disrupted, and zona occludens 1 (ZO-1) was downregulated after I/R injury. BM MSCs reduced intestinal mucosal barrier destruction, ZO-1 downregulation, and TJ disruption. The morphological abnormalities after intestinal I/R injury positively correlated with serum TNF-α levels. Administration of anti-TNF-α IgG or anti-TNF-α receptor 1 antibodies attenuated the intestinal ultrastructural changes, ZO-1 downregulation, and TJ disruption. CONCLUSION: Altered serum TNF-α levels play an important role in the ability of BM MSCs to protect against intestinal I/R injury. | Zhong-Yang Shen Jing Zhang Hong-Li Song Wei-Ping Zheng | 2013 | World Journal of Gastroenterology2013，19，23： | 23 |
| 7 | Phytoplankton Assemblage of Yangtze River Estuary and the Adjacent East China Sea in Summer, 2004显示文摘巡航与包括生物、化学、物理的题目获得长江河口的学科交差的理解的意愿从迟了的 8 月被进行到 2004 年 9 月上旬。水样品分析显示全部的浮游植物种类丰富是 137。他们, 81 在 Bacillariophyta 和 48 inPyrrophyta 被发现,为 59.1% 和 35.0% 的财务分别地。房间许多表水取样的一般水准是 8.8 x 10 ~ 4 房间L^(有最大值的-1),, 102.9 x 10 ~ 4 房间L^(在区域遇到的-1)( 31.75 °N , 122.33 °E )并且最小, 0.2 x 10 ~ 4 房间L^(-1),在( 30.75 °N , 122.17 °E )。Thedominant 种类至多驻扎是 Skeletonema 肋前缘弦音器和 Proboscia alata f。有 0.35 和 0.27 的优势的 gracillima。竖直分布分析显示房间丰富和优势种的明显的层化在 5, 18 和 33 .Shannon 牛肉熏香肠索引的代表性的车站被发现,浮游植物集合的平均与房间丰富介绍了否定关联,与出现在的最佳(30.75 ° N, 122.67 ° E ) 。为在这个区域的浮游植物花蕾主要根据通过河量的环境变量,硝酸盐的提高的营养素,硅酸盐和磷酸盐的 PCAanalysis 负责。 | LUAN Qingshan SUN Jun SHEN Zhilian SONG Shuqun WANG Min | 2006 | Journal of Ocean University of China2006，5，2： | 22 |
| 8 | Effects of Adipose-derived Mesenchymal Stem Cell Exosomes on Corneal Stromal Fibroblast Viability and Extracellular Matrix Synthesis显示文摘 | Ting Shen Qing-Qing Zheng Jiang Shen Qiu-Shi Li Xing-Hui Song Hong-Bo Luo Chao-Yang Hong Ke Yao | 2018 | Chinese Medical Journal2018，，6： | 23 |
| 9 | Trends in particulate matter and its chemical compositions in China from 2013–2017显示文摘Accurate determination of the atmospheric particulate matter mass concentration and chemical composition is helpful in exploring the causes and sources of atmospheric enthalpy pollution and in evaluating the rationality of environmental air quality control strategies.Based on the sampling and chemical composition data of PM2.5 in different key regions of China in the CARE-China observation network,this research analyzes the environmental air quality data released by the China National Environmental Monitoring Centre during the studied period to determine the changes in the particulate matter mass concentration in key regions and the evolution of the corresponding chemical compositions during the implementation of the Action Plan for Prevention and Control of Air Pollution from 2013-2017.The results show the following.(1)The particulate matter mass concentration in China showed a significant downward trend;however,the PM2.5 annual mass concentration in 64%of cities exceeds the New Chinese Ambient Air Quality Standard(CAAQS)GradeⅡ(GB3095-2012).The region to the east of the Taihang Mountains,the Fenhe and Weihe River Plain and the Urumqi-Changji regions in Xinjiang,all have PM2.5 concentration loading that is still high,and heavy haze pollution occurred frequently in the autumn and winter.(2)During the heavy pollution in the autumn and winter,the concentrations of sulfate and organic components decreased significantly.The mean SO42-concentration in PM2.5 decreased by 76%,12%,81%and 38%in Beijing-Tianjin-Hebei(BTH),the Pearl River Delta(PRD),the Sichuan-Chongqing region(SC)and the Fenhe and Weihe River Plain,respectively.The mean organic matter(OM)concentration decreased by 70%,44%,48%and 31%,respectively,and the mean concentration of NH4+decreased by 68%,1.6%,38%and 25%,respectively.The mean elemental carbon(EC)concentration decreased by 84%and 20%in BTH and SC,respectively,and it increased by 61%and 11%in the PRD and Fenhe and Weihe River Plain,respectively.The mean concentration of mineral and unresolved chemical components(MI)dropped by 70%,24%and 13%in BTH,the PRD and the Fenhe and Weihe River Plain,respectively.The change in the PM2.5 chemical composition is consistent with the decrease of the PM2.5mass concentration.(3)In 2015,the mean OM concentration contributions to fine particles and coarse particles were 13-46%and 46-57%,respectively,and the mean MI concentration contributions to fine particles and coarse and particles were 31-60%and 39-73%,respectively;these values are lower than the 2013 values from the key regions,which is the most important factor behind the decrease of the particulate matter mass concentration.From 2013 to 2015,among the chemical components of different particle size fractions,the peak value of the coarse particle size fraction decreased significantly,and the fine particle size fractions of SO42-,NO3-,and NH4+decreased with the decrease of the particulate matter mass concentration in different particle size fractions.The fine-particle size peaks of SO42-,NO3-and NH4+shifted from 0.65-1.1μm to the finer size range of0.43-0.65μm during the same time frame. | Yuesi WANG Wenjie LI Wenkang GAO Zirui LIU Shili TIAN Rongrong SHEN Dongsheng JI Shuai WANG Lili WANG Guiqian TANG Tao SONG Mengtian CHENG Gehui WANG Zhengyu GONG Jiming HAO Yuanhang ZHANG | 2019 | Science China Earth Sciences2019，62，12： | 23 |
| 10 | The second natural gas hydrate production test in the South China Sea显示文摘Clayey silt reservoirs bearing natural gas hydrates(NGH)are considered to be the hydrate-bearing reservoirs that boast the highest reserves but tend to be the most difficult to exploit.They are proved to be exploitable by the first NGH production test conducted in the South China Sea in 2017.Based on the understanding of the first production test,the China Geological Survey determined the optimal target NGH reservoirs for production test and conducted a detailed assessment,numerical and experimental simulation,and onshore testing of the reservoirs.After that,it conducted the second offshore NGH production test in 1225 m deep Shenhu Area,South China Sea(also referred to as the second production test)from October 2019 to April 2020.During the second production test,a series of technical challenges of drilling horizontal wells in shallow soft strata in deep sea were met,including wellhead stability,directional drilling of a horizontal well,reservoir stimulation and sand control,and accurate depressurization.As a result,30 days of continuous gas production was achieved,with a cumulative gas production of 86.14×104 m3.Thus,the average daily gas production is 2.87×10^4 m^3,which is 5.57 times as much as that obtained in the first production test.Therefore,both the cumulative gas production and the daily gas production were highly improved compared to the first production test.As indicated by the monitoring results of the second production test,there was no anomaly in methane content in the seafloor,seawater,and atmosphere throughout the whole production test.This successful production test further indicates that safe and effective NGH exploitation is feasible in clayey silt NGH reservoirs.The industrialization of hydrates consists of five stages in general,namely theoretical research and simulation experiments,exploratory production test,experimental production test,productive production test,and commercial production.The second production test serves as an important step from the exploratory production test to experimental production test. | Jian-liang Ye Xu-wen Qin Wen-wei Xie Hai-long Lu Bao-jin Ma Hai-jun Qiu Jin-qiang Liang Jing-an Lu Zeng-gui Kuang Cheng Lu Qian-yong Liang Shi-peng Wei Yan-jiang Yu Chunsheng Liu Bin Li Kai-xiang Shen Hao-xian Shi Qiu-ping Lu Jing Li Bei-bei Kou Gang Song Bo Li He-en Zhang Hong-feng Lu Chao Ma Yi-fei Dong Hang Bian | 2020 | China Geology2020，3，2： | 22 |
| 11 | Ratios of T-helper 2 Cells to T-helper 1 Cells and Cytokine Levels in Patients with Hepatitis B显示文摘 | Ming-Hui Li Dan Zhang Lu Zhang Xiao-Jing Qu Yao Lu Ge Shen Shu-Ling Wu Min Chang Ru-Yu Liu Lei-Ping Hu Hong-Xiao Hao Wen-Hao Hua Shu-Jing Song Gang Wan Shun-Ai Liu Yao Xie | 2017 | Chinese Medical Journal2017，，15： | 21 |
| 12 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 13 | A step metallogenetic model for gold deposits in the northwestern Shandong Peninsula, China显示文摘Based on our study of the nature and variation of mineralization-controlling faults in their deep extension and the spatial distribution of gold orebodies in the northwestern Shandong Peninsula (NSP), here we propose that the gold deposits in the Jiaojia gold belt are controlled by listric faults and the gold deposits in the NSP are controlled by extensional fault system. We also suggest that there is a multiple mineralization space in the deep part of the NSP gold belt. As steps or benches occur along the deep extention of the listric fault where its dipping angle changes from steep to gentle, a number of gold deposits are correspondingly distributed as a steplike pattern, which is named as a step metallogenetic model for gold deposits in the NSP. The gold deposits in Shandong Peninsula were formed in the Cretaceous when the crust in the region experienced extention and thinning following the strong collision between North China Plate and Yangtze Plate during the Triassic. Crust anataxis, fluid remobilization, and extention-detachement of the crust are the leading factors for the gold mineralization. | SONG MingChun YI PiHou XU JunXiang CUI ShuXue SHEN Kun JIANG HongLi YUAN WenHua WANG HuaJiang | 2012 | Science China Earth Sciences2012，55，6： | 21 |
| 14 | Dendritic Cells Transduced with SOCS1 Gene Exhibit Regulatory DC Properties and Prolong Allograft Survival显示文摘SOCS1 is a key regulator of cytokine signaling and is important for maintaining balance in the immune system. It is thought to participate in negative feedback loops in cytokine signaling and may be an important signal for the regulation of dendritic cell (DC) maturation. However, it remains unclear whether DCs transduced with SOCS1 exhibit characteristics of regulatory DCs and induce allogeneic T-cell hyporesponsiveness. In this study, we constructed adenoviral vector coding SOCS1 (Ad-SOCS1) that can efficiently increase SOCS1 gene expression in bone marrow-derived dendritic cells. DCs transduced with Ad-SOCS1 (DC-SOCS1) expressed low levels of costimulatory and MHC molecules, were resistant to maturation and activation stimulation, induced allogeneic T-cell hyporesponsiveness, and promoted the generation of regulatory-like T cells in vitro. DC-SOCS1 pretreatment significantly prolonged the survival of allografts and led to a substantial increase in the generation of regulatory T cells. Our data suggest that SOCS1 inhibits DC maturation and induces regulatory DC generation, therefore possessing therapeutic potential to prevent rejection in organ transplantation. | Hong Fu Shaohua Song Fang Liu Zhijia Ni Xiaoyun Shen Liang Xiao Guoshan Ding Quanxing Wang | 2009 | Cellular & Molecular Immunology2009，6，2： | 20 |
| 15 | Prognostic analysis of patients with gastrointestinal stromal tumors: a single unit experience with surgical treatment of primary disease显示文摘背景胃肠的 stromal 肿瘤(大意) ,胃肠的道的间充质的肿瘤的最普通的类型,是一个最近公认的肿瘤。大意的生物行为是高度可变的。外科的切除术为大意仍然是主要治疗。在这研究,我们回顾地与 181 个大意病人一起分析了我们的外科的经验决定治疗和病理学的特征和这些大意 patients.Methods 的预后因素的效果在 Ren 收到了在1999年1月和2007年12月之间的外科的切除术的有大意的 181 个病人的 clinicopathological 特征和后续数据 Ji 医院回顾地被考察。包括 CD117 (工具包) 的 Immunohistochemical 污点, CD34,和另外的标记被使用。肿瘤尺寸,有丝分裂的索引和另外的病理学的参数被记录。根据 NIH 风险组层化系统的一致基于最大的肿瘤尺寸和有丝分裂的索引(每 50 高力量地),肿瘤被分类进 very-low-risk 组( 15 个肿瘤,8.3%),低风险的组( 48 ,26.5%),中间风险的组( 52 ,28.7%)并且高风险的组( 66 ,36.5%)。预示的因素被考克斯分析包括年龄,性别,肿瘤尺寸,肿瘤地点,有丝分裂的索引, NIH 范畴和外科的过程分析。结果(59.1%) 107 181 个肿瘤位于胃, 51 (28.2%) 在小肠, 9 (5.0%) 在冒号和直肠,并且 14 (7.7%) 在另外的地点包括网膜和肠系膜。病人的中部的年龄是 58 (范围, 24-84 ) 年,和 102 个病人(56.4%) 是男性的。肿瘤尺寸从 0.5 ~ 30 厘米,当吝啬的尺寸是 7.02 厘米时。转移在 7 个病人被发现。(97.2%) 176 181 个病人经历了激进的切除术,并且在他们之中, 26 个病人与邻近的机关支持者一起收到了广泛的切除术到肿瘤。为在 immunostaining 的 KIT 蛋白质(CD117 ) 的积极的率是 94.5%(171/181 ) ,当那为 CD34 是 86.2% 时(156/181 ) 。1- , 3- ,和 181 个病人的 5 年的幸存率被估计分别地是 95.2% , 87.9% 和 78.5% 。在 multivisceral 切除术的年龄,肿瘤尺寸,肿瘤地点,有丝分裂的索引, NIH 范畴,和存在或缺席有重要差别(P < 0.05 ) 。但是在在这些组之间的性别没有重要差别。比例的模型揭示了的考克斯危险那个先进临床的阶段和大肿瘤尺寸贡献了更坏的预后。因为复发和转移或高复发风险,与 imatinib 被对待的病人显示出外科的切除术是的稳定的 disease.Conclusions 为主要大意的治疗的标准答案。NIH 分类简单、有效评估大意行为和预后。指向的治疗象 imatinib 那样,一个工具包酷氨酸 kinase 禁止者,可以在大意的治疗起一个重要作用。 | CAO Hui ZHANG Yun WANG Ming SHEN Dan-ping SHENG Zhi-yong NI Xing-zhi WU Zhi-yong LIU Qiang SHEN Yan-ying SONG Yan-yan | 2010 | Chinese Medical Journal2010，，2： | 20 |
| 16 | Growth inhibition induced by short hairpin RNA to silence survivin gene in human pancreatic cancer cells显示文摘BACKGROUND:Survivin is known to be overexpressed in various human malignancies,including pancreatic cancer,and mediates cancer cell proliferation and tumor growth,so the regulation of this molecule could be a new strategy for treating pancreatic cancer.In this study,short hairpin RNAs (shRNAs) specific to survivin were introduced into human pancreatic cancer Patu8988 cells to investigate the inhibitory effects on survivin expression and cell proliferation in vitro and in vivo.METHODS:Three kinds of shRNA specific to the survivin gene were designed and cloned into eukaryotic expression plasmid pGenesil-1 vector.Subsequently the recombinant plasmids were transfected into human pancreatic cancer Patu8988 cells with lipfectamine TM 2000 reagent.The mRNA and protein expressions of survivin in the transiently transfected Patu8988 cells were determined by RT-PCR,flow cytometry,and Western blotting analysis.The proliferation inhibition rates of stably transfected Patu8988 cells were determined by MTT assay.The antitumor activities of the three kinds of survivin-shRNA plasmids were evaluated in BALB/c nude mice inoculated with Patu8988 cells and bearing human pancreatic cancer.RESULTS:The three survivin-shRNA plasmids named pGenesil1-survivin-1,pGenesil-1-survivin-2 and pGenesil-1-survivin-1+2 (with double interfering RNA sites) were successfully constructed,and were confirmed by restriction enzyme cutting and sequencing.At 48 hours after transfection,the expression of survivin mRNA and protein was inhibited in Patu8988 cells transfected with pGenesil-1-survivin-1,pGenesil-1-survivin-2,and pGenesil-1-survivin-1+2 when compared with that of either pGenesil-1-NC (with scrambled small interfering RNA) transfected cells or control cells (P<0.05).The MTT results showed that the proliferation rates of Patu8988 cells stably transfected with survivin-shRNA plasmids were reduced when compared with that of either pGenesil-1-NC transfected cells or control cells (P<0.01).Furthermore,when Patu8988 cells stably transfected with survivin-shRNA were injected into BALB/c nude mice,tumor growth was dramatically lower and the tumor was smaller than that of either pGenesil-1-NC transfected cells or control cells (P<0.01).The inhibitory effect of pGenesil1-survivin-1 was the best among the three kinds of survivinshRNA plasmids,but no combination of inhibitory effects was found in pGenesil-1-survivin-1+2.CONCLUSIONS:shRNAs specific to survivin have gene silencing effects and inhibit pancreatic cancer cell proliferation.shRNA activity against survivin could be of potential value in gene therapy for pancreatic cancer.However,shRNAs with double combining sites did not significantly enhance the interference compared with single site shRNAs,therefore further studies on this are needed. | Shen, Yong-Mei Yang, Xiao-Chun Song, Miao-Li Qin, Chen-Hao Yang, Chen Sun, Yi-Hui | 2010 | Hepatobiliary & Pancreatic Diseases International2010，9，1： | 18 |
| 17 | Inhibitory effects of tanshinone Ⅱ-A on invasion and metastasis of human colon carcinoma cells显示文摘 | Yun-feng SHAN Xian SHEN Yuan-kang XIE Ji-cai CHEN Hong-qi SHI Zheng-ping YU Qi-tong SONG Men-tao ZHOU Qi-yu ZHANG | 2009 | Acta Pharmacologica Sinica2009，30，11： | 18 |
| 18 | Effects of electroacupuncture at Zusanli(ST36) on inflammatory cytokines in a rat model of smokeinduced chronic obstructive pulmonary disease显示文摘OBJECTIVE:Improvement in lung function was reported after acupuncture treatment of chronic obstructive pulmonary disease(COPD),but little is known about the underlying mechanisms. Because an immune response imbalance could be seen in COPD,we hypothesize that electroacupuncture(EA) may play a role in regulating inflammatory cytokines and contribute to lung protection in a rat model of smoke-induced COPD. METHODS:A COPD model using male Sprague-Dawley rats exposed to cigarette smoke was established.The rats were randomly divided into four groups(control,sham,COPD,and COPD plus EA),and COPD model was evaluated by measuring pulmonary pathological changes and lung function.EA was applied to the acupuncture point Zusanli(ST36) for 30 min/d for 14 d in sham and COPD rats.Bronchoalveolar lavage fluid(BALF) was used to measure levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and malonaldehyde(MDA). RESULTS:Compared with the control rats,COPD rats had significant changes in lung resistance(R_L) and lung compliance(C_L)(both P<0.01),bronchi and bronchiole airway obstruction(P<0.01),and levels of MDA,TNF-α,and IL-1β(P<0.01).There were no significant differences between the control and the sham groups.Compared with the COPD rats,the COPD plus EA rats had decreased R_L and increased C_L(both P<0.05),and reduced bronchi and bronchiole airway obstruction(P<0.05,P<0.01,respectively),while levels of TNF-α,IL-1β, and MDA in BALF were lowered(P<0.05 and P<0.01,respectively).However,TNF-αand IL-1βlevels of the EA group rats remained higher than those of the control group(P<0.05). CONCLUSION:EA at ST36 can reduce lung injury in a COPD rat model,and beneficial effects may be related to down-regulation of inflammatory cytokines.The anti-inflammatory and antioxidant effects mav prolong the clinical benefit of EA. | Wen-ye Geng Zi-bing Liu Na-na Song Gui-hong Zhang Wei-zhong Jin Wang Zhou Li Li Yin-xiang Cao Da-nian Zhu Lin-lin Shen | 2013 | Journal of Integrative Medicine2013，11，3： | 18 |
| 19 | A comparative study between Embosphere~ and conventional transcatheter arterial chemoembolization for treatment of unresectable liver metastasis from GIST显示文摘Objective:Transcatheter arterial chemoembolization(TACE) is a standard treatment for hepatocellular carcinoma(HCC) and/or some unresectable liver metastasis tumors.Hypervascular liver metastatic lesions such as metastasis from gastrointestinal stromal tumor(GIST) are an indication for transcatheter arterial embolization(TAE).The purpose of this study was to evaluate the efficacy and safety of Embosphere~-TAE(Embo-TAE) in comparison with conventional TACE(cTACE) for the treatment of liver metastasis from GIST.Methods:A total of 45 patients who underwent TACE between Aug 2008 and Feb 2013 were enrolled.Patients with GIST who underwent TAE with Embosphere~(n=19) were compared with controls who received cTACE(n=26).The primary end points were treatment response and treatment-related adverse events.The secondary end points were progression-free survival(PFS) and overall survival(OS).Results:The treatment response of Embo-TAE group was significantly higher than that of the cTACE group(P<0.001).The PFS was significantly better in the Embosphere~-group than in the cTACE group(56.6 and 42.1 weeks,respectively;P=0.003).However,there was no statistically significant difference in liver toxicity between the two groups(P>0.05).The median OS in the Embo-TAE group was longer than that in the cTACE group(74.0 weeks,95% CI:68.2-79.8 vs.61.7 weeks,95% CI:56.2-67.2 weeks)(unadjusted P=0.045).The use of Embo-TAE significantly reduced the risk of death in patients with GIST with liver metastases according to the Cox proportional hazards regression model [hazard ratio(HR):0.149;95% CI:0.064-0.475].Conclusions:TAE with Embosphere~ showed better treatment response and delayed tumor progression compared with cTACE.There was no significant difference in treatment-related hepatic toxicities.EmboTAE thus appears to be a feasible and promising approach in the treatment of liver metastasis from GIST. | Guang Cao Xu Zhu Jian Li Lin Shen Renjie Yang Hui Chen Xiaodong Wang Song Gao Haifeng Xu Linzhong Zhu Peng Liu Jianhai Guo | 2014 | Chinese Journal of Cancer Research2014，26，1： | 18 |
| 20 | Kinetics of Hepatitis B Surface Antigen Level in Chronic Hepatitis B Patients who Achieved Hepatitis B Surface Antigen Loss during Pegylated Interferon Alpha-2a Treatment显示文摘 | Ming-Hui Li Lu Zhang Xiao-Jing Qu Yao Lu Ge Shen Shu-Ling Wu Min Chang Ru-Yu Liu Lei-Ping HU Zhen-Zhen Li Wen-Hao Hua Shu-Jing Song Yao Xie | 2017 | Chinese Medical Journal2017，，5： | 18 |
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