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| 1 | Relocation of the M8.0 Wenchuan earthquake and its aftershock sequence显示文摘We relocated M8.0 Wenchuan earthquake and 2706 aftershocks with M≥2.0 using double-difference algorithm and obtained relocations of 2553 events. To reduce the influence of lateral variation in crustal and upper mantle velocity structure, we used different velocity models for the east and west side of Longmenshan fault zone. In the relocation process, we added seismic data from portable seismic sta-tions close to the shocks to constrain focal depths. The precisions in E-W, N-S, and U-D directions after relocation are 0.6, 0.7, and 2.5 km respectively. The relocation results show that the aftershock epi-centers of Wenchuan earthquake were distributed in NE-SW direction, with a total length of about 330 km. The aftershocks were concentrated on the west side of the central fault of Longmenshan fault zone, excluding those on the north of Qingchuan, which obviously deviated from the surface fault and passed through Pingwu-Qingchuan fault in the north. The dominant focal depths of the aftershocks are between 5 and 20 km, the average depth is 13.3 km, and the depth of the relocated main shock is 16.0 km. The depth profile reveals that focal depth distribution in some of the areas is characterized by high-angle westward dipping. The rupture mode of the main shock features reverse faulting in the south, with a large strike-slip component in the north. | HUANG Yuan WU JianPing ZHANG TianZhong ZHANG DongNing | 2008 | Science China Earth Sciences2008，51，12： | 64 |
| 2 | Cloning and Characterization of miRNAs and Their Targets, Including a Novel miRNA-Targeted NBS-LRR Protein Class Gene in Apple (Golden Delicious)显示文摘MicroRNA (miRNA ) 在植物作为基因表示的一个重要管理者出现了。146 miRNAs 从苹果被识别(Malus domestica cv。金色美味) 由定序的生物信息的分析和 RNA 图书馆。从这些, 135 被保存, 11 是新奇 miRNAs。目标分析预言了新奇 miRNAs 之一, Md-miRLn11 (Malus domestica microRNA Ln11 ) ,指向了一个苹果核苷酸绑定地点(NBS ) 充满白氨酸的重复(LRR ) 班蛋白质编码基因(Md-NBS ) 。5 赛跑试金证实了 Md-miRLn11 的能力在 1112-nt 位置劈开 Md-NBS。Md-miRLn11 和 Md-NBS 的表示的分析在在 40 个苹果变化的最佳侵略时期期间证明在抵抗变化的 Md-NBS 基因的表示比在易受影响的变化高,与为 Md-miRLn11 的一个反的模式。从抵抗苹果变化 JiGuan 的幼苗被用来执行 Agrobacterium 渗入试金,然后与苹果叶点疾病接种。结果在 JiGuan 苹果显示出疾病抵抗的清楚的衰落。相反,与 Md-NBS 基因渗入的易受影响的变化富士在疾病抵抗显示出重要增加。基于上述结果,我们建议 Md-miRLn11 特别地在病原体感染的条件下面调整 Md-NBS 基因表示,并且指向 P 环地点的 Md-miRLn11 可以在木质的植物调整许多 NBSLRR 蛋白质类基因。 | Chao Ma You Lu Songlin Bai Wennan Zhang Xuwei Duan Dong Meng Zhigang Wang Aide Wang Zongshan Zhou Tianzhong Li | 2014 | Molecular Plant2014，7，1： | 19 |
| 3 | An MYB Transcription Factor from Malus xiaojinensis Has a Potential Role in Iron Nutrition显示文摘Regulation of iron uptake and use is critical for plant survival and growth. We isolated an MYB gene from Malus xiaojinensis named MxMYB1, which is induced under Fe-deficient conditions. Expression of MxMYB1 was upregulated by Fe starvation in the roots but not in leaves, suggesting that MxMYB1 might play a role in iron nutrition in roots. Transgenic Arabidopsis plants expressing MxMYB1 exhibited lower iron content as compared with wild type plants under both Fe-normal (40 μM) and Fe-deficient conditions (Fe omitted and Ferrozine 300 μM). However, the contents of Cu, Zn and Mn were not changed in these transgenic plants. Gene chip and real-time polymerase chain reaction analyses indicated that the expression of two Fe-related genes encoding an iron transporter AtIRT1 and an iron storage protein ferritin AtFER1 might be negatively regulated by MxMYB1 as the expression levels of these genes were lower in MxMYB1 expressing transgenic Arabidopsis plants as compared with wild type plants under both Fe-normal and Fe-deficient conditions. These results suggest that MxMYB1 may function as a negative regulator of iron uptake and storage in plants. | Jie Shen Xuefeng Xu Tianzhong Li Dongmei Cao ZhenhaiHan | 2008 | Journal of Integrative Plant Biology2008，50，10： | 5 |
| 4 | A novel miRNA negatively regulates resistance to Glomerella leaf spot by suppressing expression of an NBS gene in apple显示文摘Glomerella leaf spot(GLS)of apple(Malus×domestica Borkh.),caused by Glomerella cingulata,is an emerging fungal epidemic threatening the apple industry.Little is known about the molecular mechanism underlying resistance to this devastating fungus.In this study,high-throughput sequencing technology was used to identify microRNAs(miRNAs)involved in GLS resistance in apple.We focused on miRNAs that target genes related to disease and found that expression of a novel miRNA,Md-miRln20,was higher in susceptible apple varieties than in resistant ones.Furthermore,its target gene Md-TN1-GLS exhibited the opposite expression pattern,which suggested that the expression levels of Md-miRln20 and its target gene are closely related to apple resistance to GLS.Furthermore,downregulation of MdmiRln20 in susceptible apple leaves resulted in upregulation of Md-TN1-GLS and reduced the disease incidence.Conversely,overexpression of Md-miRln20 in resistant apple leaves suppressed Md-TN1-GLS expression,with increased disease incidence.We demonstrated that Md-miRln20 negatively regulates resistance to GLS by suppressing Md-TN1-GLS expression and showed,for the first time,a crucial role for miRNA in response to GLS in apple. | Yi Zhang Qiulei Zhang Li Hao Shengnan Wang Shengyuan Wang Wenna Zhang Chaoran Xu Yunfei Yu Tianzhong Li | 2019 | Horticulture Research2019，6，1： | 4 |
| 5 | A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2显示文摘The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus,and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.On the other hand,several concerning SARS-CoV-2 variants,including Omicron,are required to be identified as soon as the samples are identified as‘positive’.Unfortunately,a traditional PCR test does not allow their specific identification.Herein,for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing),which combines an optical sensing technology-surface plasmon resonance(SPR)with the‘gene scissors’clustered regularly interspaced short palindromic repeat(CRISPR)technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants.MOPCS is a low-cost,CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA,without the need for amplification,within 38 min from sample input to results output,and achieve a limit of detection of 15 fM.MOPCS achieves a highly sensitive analysis of SARS-CoV-2,and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron)and BA.1(a subtype of Omicron).This platform was also used to analyze some recently collected patient samples from a local outbreak in China,identified by the Centers for Disease Control and Prevention.This innovative CRISPR-empowered SPR platform will further contribute to the fast,sensitive and accurate detection of target nucleic acid sequences with single-base mutations. | Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar AAl-Hartomy Swelm Wageh Abdullah GAl-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang | 2022 | National Science Review2022，9，8： | 3 |
| 6 | Two pathogenesis-related proteins interact with leucine-rich repeat proteins to promote Alternaria leaf spot resistance in apple显示文摘Alternaria leaf spot in apple(Malus x domestica),caused by the fungal pathogen Alternaria alternata f.sp.mali(also called A.mali),is a devastating disease resulting in substantial economic losses.We previously established that the resistance(R)protein MdRNL2,containing a coiled-coil,nucleotide-binding,and leucine-rich repeat(CC R-NB-LRR)domain,interacts with another CC R-NB-LRR protein,MdRNL6,to form a MdRNL2–MdRNL6 complex that confers resistance to A.mali.Here,to investigate the function of the MdRNL2–MdRNL6 complex,we identified two novel pathogenesis-related(PR)proteins,MdPR10-1 and MdPR10-2,that interact with MdRNL2.Yeast two-hybrid(Y2H)assays and bimolecular fluorescence complementation(BiFC)assays confirmed that MdPR10-1 and MdPR10-2 interact with MdRNL2 and MdRNL6 at the leucine-rich repeat domain.Transient expression assays demonstrated that accumulation of MdPR10-1 and MdPR10-2 enhanced the resistance of apple to four strains of A.mali that we tested:ALT1,GBYB2,BXSB5,and BXSB7.In vitro antifungal activity assays demonstrated that both the proteins contribute to Alternaria leaf spot resistance by inhibiting fungal growth.Our data provide evidence for a novel regulatory mechanism in which MdRNL2 and MdRNL6 interact with MdPR10-1 and MdPR10-2 to inhibit fungal growth,thereby contributing to Alternaria leaf spot resistance in apple.The identification of these two novel PR proteins will facilitate breeding for fungal disease resistance in apple. | Qiulei Zhang Chaoran Xu Haiyang Wei Wenqi Fan Tianzhong Li | 2021 | Horticulture Research2021，8，1： | 2 |
| 7 | Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18显示文摘 | Guoshun Shen Ningyi Jin Mingxiao Ma Kuoshi Jin Min Zheng Tianzhong Zhuang Huijun Lu Guangze Zhu Hongtao Jin Minglan Jin Xiaowei Huo Xiaoguang Qin Ronglan Yin Chang Li Hongwen Li Yang Li Zhenzhen Han Yifeng Chen Miaomiao Jin | 2007 | Vaccine2007，，21： | 2 |
| 8 | Studies on the Attached Cultivation of Filamentous Oleaginous Microalga Tribonema minus显示文摘Attached cultivation is a promising method for microalgal biomass production. Filamentous oleaginous microalga Tribonema minus(hereafter T. minus) has shown a remarkable potential for biofuel production in terms of its high lipid content. However, the strain has only been cultivated in suspended cultivation systems including open pond and closed photobioreactors. Here, we attempted to study the attached cultivation of T. minus, which might be helpful for its scale-up cultivation and industrial applications. As the results, the optimal conditions for T. minus growth in the attached biofilm are 200 μmol photons m^-2 s^-1 of light intensity and 5% of CO2, and the maximum biomass density of 223 g m^-2 has been achieved under the light intensity. The non-woven fabric as substratum was found as the best substratum in thin layer attached bioreactor, on which the average biomass productivity of T. minus is about(9.73 ± 2.19) g m^-2)d^-1. Furthermore, two attached bioreactor systems, rotary drum and rotation disc, were designed following the light dilution strategy and introduced into T. minus cultivation. The highest footprint areal biomass productivity of these two systems is 33 and 47.1 g m^-2 d^-1, respectively, much higher than that in suspended cultivation system. The results shows that T. minus can be cultured with attached cultivation method to improve its biomass productivity. | ZHANG Yan JI Chunli ZHOU Wenjun WANG Hui WANG Junfeng LIU Tianzhong | 2020 | Journal of Ocean University of China2020，19，3： | 2 |
| 9 | Efficient Breeding and Cultivation of Type 2 Red-fleshed Apple Cultivars Using a Search System for Suitable Apple Cultivar Combination显示文摘As apple possesses self-incompatibility, knowledge of the S-genotypes is very important for apple breeding and cultivation. We investigated the S-RNase genotypes of 13 type 2 red-fleshed apple cultivars and 8 lineages. All of the investigated type 2 red-fleshed apples contained the S3-RNase allele closely linked to the red-fleshed trait. We made a search system for ovule parents to efficiently produce new type 2 red-fleshed cultivars based on the S-RNase allele information(http://www.agr.nagoya-u.ac.jp/~hort/apple/red/). More than 90% of progenies from crossing a selected white-fleshed ovule parent with a type 2 red-fleshed pollen parent are expected to show the red-fleshed phenotype. We also compiled a database of the S-RNase genotypes of more than 1 000 apple cultivars, including a survey system of cultivar combinations showing those that were fully incompatible, semicompatible and fully compatible, written in Chinese(http://www.agr.nagoya-u.ac.jp/~hort/apple/ch/) and Japanese(http://www.agr.nagoya-u.ac.jp/~hort/apple/ja/). | SHOGO Matsumoto LI Tianzhong SHUNGO Otagaki LI Yang BAI Songling | 2018 | Horticultural Plant Journal2018，4，6： | 2 |
| 10 | Comparison of growth,hydrocarbon accumulation and metabolites of Botryococcus braunii between attached cultivation and aqueous-suspension cultivation显示文摘Botryococcus braunii is a colonial green microalga which can produce extracellular hydrocarbons at a high rate,it is considered as one of the most promising feedstocks for sustainable biofuel production.However,B.braunii is generally recognized difficulties for cultivating and has limited amount of substantive scale-up and productivity assessments with conventional aqua-suspended cultivation systems(open pond and varieties of closed photobioreactors).This paper introduces a novel cultivation system based on biofilm technology,which is called“attached cultivation”.To investigate the potential of attached cultivation method,attached cultivation of B.braunii SAG 807-1 was compared with aqueous-suspension cultivation(flat plate reactors).The growth,hydrocarbon accumulation and metabolites were studied under identical conditions(e.g.temperature,light intensity,CO2 concentration).The main research results obtained are as follows:compared with conventional aqueous-suspension cultivation,the biomass productivity of B.braunii under biofilm attached cultivation was 4.78 g/(m^(2)·d),which was higher than of 4.43 g/(m^(2)·d)by aqueous-suspension,and hydrocarbon productivity of the two cultivation methods were 2.52 g/(m^(2)·d)and 2.37 g/(m^(2)·d),respectively.The contents of carbohydrate and protein were also similar.This attached cultivation method showed a new model of commercialization for the microalgae-derived biofuels. | Cheng Pengfei Wang Yan Yang Qiyong Liu Tianzhong | 2017 | International Journal of Agricultural and Biological Engineering2017，10，1： | 1 |
| 11 | Preparation of Fe 3+ -doped TiO 2 catalysts by controlled hydrolysis of titanium alkoxide and study on their photocatalytic activity for methyl orange degradation显示文摘 | Tianzhong Tong Jinlong Zhang Baozhu Tian Feng Chen Dannong He | 2007 | Journal of Hazardous Materials2007，，： | 1 |
| 12 | Image annotation techniques based on feature selection for class-pairs显示文摘 | Jianjiang Lu Ran Li Yafei Zhang Tianzhong Zhao Zining Lu | 2010 | Knowledge and Information Systems2010，，2： | 1 |
| 13 | Preparation of Ce-TiO2 catalysts by controlled hydrolysis of titanium alkoxide based on esterificatlon reaction and study on its photocatalytic activity 显示文摘 | Tong Tianzhong Zhang Jinlong Tian Baozhu | 2007 | J Colloid Interface Sci2007，315，1： | 1 |
| 14 | EXPERT SYSTEM FOR SINTER CHEMICAL COMPOSITION CONTROL BASED ON ADAPTIVE PREDICTION显示文摘In order to stabilize the sinter chemical composition,the expert system for composition control based on adaptive prediction has been developed on the basis of modern control theory and artificial intelligence.It has been verified by using real data at No.3 Sintering Plant of Anshan Iron and Steel Co.,and satisfactory results have been obtained. | Fan Xiaohui Wang Haidong Chen Jin Huang Tianzheng Yang Shinong Wang Yueli Zheng Tianzhong | 1996 | Transactions of Nonferrous Metals Society of China1996，6，1： | 1 |
| 15 | Biodiesel production from algae oil high in free acids by two-step catalytic conversion 显示文摘 | Chen Lin Liu Tianzhong Zhang Wei | 2012 | BioresourceTechnology2012，111，： | 1 |
| 16 | Utilization of simulated flue gas for cultiva-tion of Scenedesmus dimorphus显示文摘 | Jiang Yinli Zhang Wei Wang Junfeng Chen Yu Shen Shuhua Liu Tianzhong | | 0，，： | 1 |
| 17 | Preparation of Fe^3+ -doped TiO2 catalysts by controlled hydrolysis of titanium alkoxide and study on their photocatalytic activity for methyl orange degradation显示文摘 | Tianzhong Tong Jinlong Zhang Baozhu Tian | 2008 | Journal of Hazardous Materials2008，155，3： | 1 |
| 18 | Functional identification of lncRNAs in sweet cherry(Prunus avium)pollen tubes via transcriptome analysis using single-molecule long-read sequencing显示文摘Sweet cherry(Prunus avium)is a popular fruit with high nutritional value and excellent flavor.Although pollen plays an important role in the double fertilization and subsequent fruit production of this species,little is known about its pollen tube transcriptome.In this study,we identified 16,409 transcripts using single-molecule sequencing.After filtering 292 transposable elements,we conducted further analyses including mRNA classification,gene function prediction,alternative splicing(AS)analysis,and long noncoding RNA(lncRNA)identification to gain insight into the pollen transcriptome.The filtered transcripts could be matched with 3,438 coding region sequences from the sweet cherry genome.GO and KEGG analyses revealed complex biological processes during pollen tube elongation.A total of 2043 AS events were predicted,7 of which were identified in different organs,such as the leaf,pistil and pollen tube.Using BLASTnt and the Coding-Potential Assessment Tool(CPAT),we distinguished a total of 284 lncRNAs,among which 154 qualified as natural antisense transcripts(NATs).As the NATs could be the reverse complements of coding mRNA sequences,they might bind to coding sequences.Antisense transfection assays showed that the NATs could regulate the expression levels of their complementary sequences and even affect the growth conditions of pollen tubes.In summary,this research characterizes the transcripts of P.avium pollen and lays the foundation for elucidating the physiological and biochemical mechanisms underlying sexual reproduction in the male gametes of this species. | Yang Li Chuanbao Wu Chunsheng Liu Jie Yu Xuwei Duan Wenqi Fan Jing Wang Xiaoming Zhang Guohua Yan Tianzhong Li Kaichun Zhang | 2019 | Horticulture Research2019，6，1： | 1 |
| 19 | Integration process of biodiesel production from filamentous oleaginous microalgae Tribonema minus显示文摘 | Hui Wang Lili Gao Lin Chen Fajin Guo Tianzhong Liu | 2013 | Bioresource Technology2013，，： | 1 |
| 20 | Prepara- tion of Ce-TiO2 catalysts by controlled hydrolysis of titanium alkoxide based on esterifieation reaction and study on its photoeatalytic activity 显示文摘 | Tong Tianzhong Zhang Jinlong Tian Baozhu | 2007 | Journal of Colloid and Interface Science2007，315，1： | 1 |
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