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| 1 | Assessment of heavy metal pollution characteristics and human health risk of exposure to ambient PM_(2.5) in Tianjin,China显示文摘To examine the features of heavy metal pollution of PM_(2.5)(particulate matter less than 2.5 μm) in Tianjin,China,as well as the exposure risk of PM_(2.5) to human health,we analyzed ambient PM_(2.5) samples collected from a campus of Nankai University in June,August,and October 2012.The concentrations of PM_(2.5) and heavy metals(Ni,Cu,Pb,Zn,Cr,Cd,Hg,As and Mn) in PM2.5 were analyzed by gravimetric analysis and inductively coupled plasma-mass spectrometry,respectively.The results show that the heavy metals contained in PM_(2.5) were,in descending order,Cu,Zn,Pb,Mn,Cr,Ni,Cd,As,and Hg.The proportion of Cd exceeded the secondary level of National Ambient Air Quality Standard of China(GB 3095-2012) by 1.3times,while others were within the limit.Enrichment factor analysis indicated that Cu,Zn,Cd,Pb,and Hg are mainly from anthropogenic sources.Principal component analysis indicated that the main sources of the heavy metals are vehicle exhaust,chemical waste,and coal-burning activities.The nine heavy metals which may cause health issues by exposure through the human respiratory system and should be further examined are Cr,Cd,As,Ni,Cu,Pb,Mn,Zn,and Hg,in the order of decreasing risk levels.With reference to the U.S.EPA standard the risk levels of all nine metals were below the acceptable level(10^(-6)/year). | Peifei Chen Xiaohui Bi Jiaqi Zhang Jianhui Wu Yinchang Feng | 2015 | Particuology2015，13，3： | 43 |
| 2 | Using geoaccumulation index to study source profiles of soil dust in China显示文摘TSP (有空气动力学的 diameters<=100 亩 m 的大气的微粒事) 和 PM10 (有空气动力学的 diameters<=10 亩 m 的大气的微粒事) 的来源分配研究表明了土壤灰尘是在中国的这些微粒的重要来源。在这研究,土壤灰尘的污染通过一个 geoaccumulation 索引(I_(geo )) 的使用被估计。 17 个元素的集体集中侧面( Na , Mg ,艾尔, K , Ca , Ti , V , Cr , Mn , Fe ,公司, Ni , Cu , Zn , Br , Ba ,并且 Pb )被建立因为来自 15 个城市的土壤的城市的土壤 dusts.Geochemical 作文被用来代表背景城市的土壤作文。这研究的结果显示很多个城市被在两尺寸部分(TSP 和 PM10 ) 包含 Ca, Cr, Ni,和 Cu 的微粒严重地弄脏。有 Zn, Pb,公司,和 Br 的污染对中等严重(I_(geo )>2 ) 。艾尔和 Fe 集中不足够高让他们被认为沾染物。 | JI Yaqin FENG Yinchang WU Jianhui ZHU Tan BAI Zhipeng DUAN Chiqing | 2008 | Journal of Environmental Sciences2008，20，5： | 35 |
| 3 | Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis显示文摘像域的蛋白质(Mlkl ) 最近被发现与受体交往交往的混合的系 kinase 蛋白质 3 (Rip3 ) 并且导致的坏死因素(TNF ) 在有教养的房间线规划了坏死(necroptosis ) 为肿瘤必要。我们由抄写产生了 Mlkl 缺乏的老鼠像使活跃之物的受动器核酸酶(TALEN ) 调停了基因混乱和发现 Mlkl 到为象有免疫力的房间开发一样的正常老鼠开发非必需。胚胎的成纤维细胞(MEF ) 和巨噬细胞两个都显示出的 Mlkl 缺乏的老鼠抵抗到坏死然而并非 apoptotic 刺激。Mlkl 缺乏的 MEF 和巨噬细胞与处于他们激活 NF-κ 的能力的野类型的房间难区分;响应 TNF 和 lipopolysaccharides (LPS ) 的 B,英皇家空军之阶级最低之兵, JNK,和 p38 分别地。一致地, Mlkl 缺乏的巨噬细胞和老鼠展出了正常 interleukin-1β(IL-1β) ,在 LPS 处理以后的 IL-6,和 TNF 生产。Mlkl 缺乏保护老鼠免受导致天蓝色的尖锐胰腺炎的伤害,坏死相关的疾病,但是没在 polymicrobial 上有效果腐败导致吃惊的动物死亡。我们的结果在 necroptosis 为 Mlkl 的角色提供基因证据。 | Jianfeng Wu Zhe Huang Junming Ren Zhirong Zhang Peng He Yangxin Li Jianhui Ma Wanze Chen Yingying Zhang Xiaojuan Zhou Zhentao Yang Su-Qin Wu Lanfen Chen Jiahuai Han | 2013 | Cell Research2013，23，8： | 35 |
| 4 | Prognostic value of systemic immune.inflammation index in patients with gastric cancer显示文摘Background:Inflammation-based indexes have been used to predict survival and recurrence in cancer patients.Systemic immune-inflammation index(Sll) was reported to be associated with prognosis in some malignant tumors.In the present study,we aimed to explore the association between Sll and the prognosis of patients with gastric cancer.Methods:We retrospectively analyzed data from 444 gastric cancer patients who underwent gastrectomy at the First Affiliated Hospital of Sun Yat-sen University between January 1994 and December 2005.Preoperative Sll was calculated.The Chi square test or Fisher's exact test was used to determine the relationship between preoperative Sll and clinicopathologic characteristics.Overall survival(OS) rates were estimated using the Kaplan-Meier method,and the effect of Sll on OS was analyzed using the Cox proportional hazards model.Receiver operating characteristic(ROC)curves were used to compare the predictive ability of Sll,NLR,and PLR.Results:Sll equal to or higher than 660 was significantly associated with old age,large tumor size,unfavorable Borrmann classification,advanced tumor invasion,lymph node metastasis,distant metastasis,advanced TNM stage,and high carcino-embryonic antigen level,high neutrophil-lymphocyte ratio,and high platelet-lymphocyte ratio(all P<0.05).High Sll was significantly associated with unfavorable prognosis(P<0.001) and Sll was an independent predictor for OS(P=0.015).Subgroups analysis further showed significant associations between high Sll and short OS in stage Ⅰ,Ⅱ,Ⅲ subgroups(all P<0.05).Sll was superior to NLR and PLR for predicting OS in patients with gastric cancer.Conclusion:Preoperative Sll level is an independent prognostic factor for OS in patients with gastric cancer. | Kang Wang Feiyu Diao Zhijun Ye Xinhua Zhang Ertao Zhai Hui Ren Tong Li Hui Wu Yulong He Shirong Cai Jianhui Chen | 2017 | Chinese Journal of Cancer2017，36，9： | 30 |
| 5 | Secondary organic carbon quantification and source apportionment of PM_(10) in Kaifeng, China显示文摘During 2005, the filter samples of ambient PM10 from five sites and the source samples of particulate matter were collected in Kaifeng, Henan Province of China. Nineteen elements, water-soluble ions, total carbon (TC) and organic carbon (OC) contained in samples were analyzed. Seven contributive source types were identified and their contributions to ambient PM10 were estimated by chemical mass balance (CMB) receptor model. Weak associations between the concentrations of organic carbon and element carbon (EC) were observed during the sampling periods, indicating that there was secondary organic aerosol pollution in the urban atmosphere. An indirect method of 'OC/EC minimum ratio' was applied to estimate the concentration of secondary organic carbon (SOC). The results showed that SOC contributed 26.2%, 32.4% and 18.0% of TC in spring, summer-fall and winter, respectively, and the annual average SOC concentration was 7.07 μg/m3, accounting for 5.73% of the total mass in ambient PM10. The carbon species concentrations in ambient PM10 were recalculated by subtracting SOC concentrations from measured concentrations of TC and OC to increase the compatibility of source and receptor measurements for CMB model. | WU Lin FENG Yinchang WU Jianhui ZHU Tan BI Xiaohui HAN Bo YANG Weihong YANG Zhiqiang | 2009 | Journal of Environmental Sciences2009，21，10： | 26 |
| 6 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 7 | 恶性肿瘤骨转移及骨相关疾病临床诊疗中国专家共识(英文)显示文摘Since 1980s, palliative medicine has been an independent educational project. The development of palliative care in China started in the early 1990s. During the last 18 years, we have seen an increasing allocation of resources to palliative care, the establishment of an academic chair in palliative medicine, and more focused interest and awareness of the need for palliative care in the society at large. So the doctors in China have established principles of treatment on clinical diagnosis and treatment of malignant tumor bone metastases and bone related complications. In order to share our experience with the world, after two years’ preparation, the top-level experts in palliative medicine have written the consensus statement on clinical diagnosis and treatment of malignant tumor bone metastases and bone related complications. The overview includes not only the achievements in the world, but the hands-on experience in clinic, especially the experience in combination of TCM and Western medicine. We hope that the publication of this consensus statement will contribute to the palliative medicine development in this field, thus bring benefits to the majority of patients. | Shiying Yu Zefei Jiang Li Zhang Xiaohui Niu Lvhua Wang Ning Wu Jianhui Ma | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，1： | 15 |
| 8 | Major advances in studies of the physical geography and living environment of China during the past 70 years and future prospects显示文摘The natural environment provides material essentials for human survival and development. The characteristics,processes, regional differentiation and forcing mechanisms of the elements of the natural environment(e.g. geomorphology,climate, hydrology, soil, etc.) are the main objects of research in physical geography. China has a complex natural environment and huge regional differentiation and therefore it provides outstanding reserach opportunities in physical geography. This review summarizes the most important developments and the main contributions of research in the physical geography and human living environment in China during the past 70 years. The major topics addressed are the uplift of the Tibetan Plateau and the evolution of its cryosphere, the development of fluvial systems, the acidification of the vast arid region of the Asian interior, variations in the monsoon and westerly climate systems on multiple timescales, the development of lakes and wetlands, the watershed system model, soil erosion, past human-environment interactions, biogeography, and physical geographic zonality. After briefly introducing international research developments, we review the history of research in physical geography in China, focusing on the major achievements and major academic debates, and finally we summarize the status of current research and the future prospects. We propose that in the context of the national demand for the construction of an ecological civilization, we should make full use of the research findings of physical geography, and determine the patterns and mechanisms of natural environmental processes in order to continue to promote the continued contribution of physical geography to national development strategies, and to further contribute to the theory of physical geography from a global perspective. | Fahu CHEN Bojie FU Jun XIA Duo WU Shaohong WU Yili ZHANG Hang SUN Yu LIU Xiaomin FANG Boqiang QIN Xin LI Tingjun ZHANG Baoyuan LIU Zhibao DONG Shugui HOU Lide TIAN Baiqing XU Guanghui DONG Jingyun ZHENG Wei YANG Xin WANG Zaijun LI Fei Wang Zhenbo HU Jie WANG Jianbao LIU Jianhui CHEN Wei HUANG Juzhi HOU Qiufang CAI Hao LONG Ming JIANG Yaxian HU Xiaoming FENG Xingguo MO Xiaoyan YANG Dongju ZHANG Xiuhong WANG Yunhe YIN Xiaochen LIU | 2019 | Science China Earth Sciences2019，62，11： | 11 |
| 9 | Bio-geomorphologic features and growth process of Tamarix nabkhas in Hotan River Basin, Xinjiang显示文摘Tamarix nabkha 是最普遍的 nabkhas 之一,在中国的干旱区域散布。在实验室基于户外的观察和模拟实验,在这份报纸的分析在 Hotan 河的中间、更低的活动范围指生物 geomorphologic 特征和 Tamarix nabkhas 的生长过程, Xinjiang。并且结果显示在学习区域的 Tamarix 的生态的类型基于 Populus Diversifolia 森林和灌木的深土壤是一种 Tugaic 土壤产地。这类产地能被划分成在微分发达阶段期间表明 Tamarix nabkhas 的生态的环境的特征的三种亚产地。同时, Tamarix nabkha 能在地面,和它的根和茎上在风沙流动上施加加强的骚乱电流不仅有发芽的强壮的潜力但是风侵蚀忍耐是特征的,被沙并且分别地要埋葬的电阻木质化的分支僵硬的流氓,为它有相当更长的一生。因此, Tamarix nabkha 影响的风速度侧面与 Phragmites nabkha 和 Alhagi nabkha 不同。并且风流动的结构对风神的沙在里面 / 附近 Tamarix 积累有益灌木,它能与更高平均的坡度和生活的更长的频率创造唯一的 Tamarix nabkhas。在区域的 Tamarix nabkha 进化经历了三个阶段:生长舞台,成熟、稳定的阶段和凋谢的舞台。在每个阶段,词法特征和 Tamarix nabkha 的似地球形状的过程由于在 nabkha 和风神的沙流动之间的差异的相互作用是不同的。 | LI Zhizhong WU Shengli CHEN Shujiang CHEN Xiuling JIN Jianhui LIU QI | 2010 | Journal of Geographical Sciences2010，20，2： | 10 |
| 10 | WheatOmics:A platform combining multiple omics data to accelerate functional genomics studies in wheat显示文摘Dear Editor,Mold-breaking progress in whole-genome sequencing and rapid accumulation of multi-omics data have revolutionized the research strategies of functional genomics in wheat(Wang et al.,2018).However,how to access these vast multi-omics data and to extract key information on genes of in-terest,is still challenging for most wet-lab or field wheat re-searchers who have little bioinformatic experiences and cannot access the expensive computational resources.Here,we pre-sent WheatOmics(http://wheatomics.sdau.edu.cn/,previously designated as Triticeae Multi-omics Center,http://202.194.139.32/),a free,web-accessible,and user-friendly platform.WheatOmics not only empowers the effective access to the visualized multi-omics data of user-interested genes but also offers several distinctive and practical toolkits that can ease almost every aspect of wheat functional genomics studies(Figure 1A). | Shengwei Ma Meng Wang Jianhui Wu Weilong Guo Yongming Chen Guangwei Li Yanpeng Wang Weiming Shi Guangmin Xia Daolin FU Zhensheng Kang Fei Ni | 2021 | Molecular Plant2021，14，12： | 9 |
| 11 | Comparative analysis between endometrial proteomes of pregnant and non-pregnant ewes during the peri-implantation period显示文摘Background:Early pregnancy failure has a profound impact on both human reproductive health and animal production.2/3 pregnancy failures occur during the peri-implantation period;however,the underlying mechanism(s)remains unclear.Well-organized modification of the endometrium to a receptive state is critical to establish pregnancy.Aberrant endometrial modification during implantation is thought to be largely responsible for early pregnancy loss.Result:In this study,using well-managed recipient ewes that received embryo transfer as model,we compared the endometrial proteome between pregnant and non-pregnant ewes during implantation period.After embryo transfer,recipients were assigned as pregnant or non-pregnant ewes according to the presence or absence of an elongated conceptus at Day 17 of pregnancy.By comparing the endometrial proteomic profiles between pregnant and non-pregnant ewes,we identified 94 and 257 differentially expressed proteins(DEPs) in the endometrial caruncular and intercaruncular areas,respectively.Functional analysis showed that the DEPs were mainly associated with immune response,nutrient transport and utilization,as well as proteasome-mediated proteolysis.Conclusion:These analysis imply that dysfunction of these biological processes or pathways of DEP in the endometrium is highly associated with early pregnancy loss.In addition,many proteins that are essential for the establishment of pregnancy showed dysregulation in the endometrium of non-pregnant ewes.These proteins,as potential candidates,may contribute to early pregnancy loss. | Haichao Zhao Linlin Sui Kai Miao Lei An Dong Wang Zhuocheng Hou Rui Wang Min Guo Zhilong Wang Jiqiang Xu Zhonghong Wu Jianhui Tian | 2015 | Journal of Animal Science and Biotechnology2015，6，3： | 8 |
| 12 | Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene (LHCGR) with superovulation traits in Chinese Holstein heifers显示文摘Background: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers. | Yong Yu Yunwei Pang Haichao Zhao Xiaoling Xu Zhonghong Wu Lei An Jianhui Tian | 2012 | Journal of Animal Science and Biotechnology2012，3，4： | 6 |
| 13 | Source apportionment of ambient PM_(10) in urban areas of Wuxi,China显示文摘A total of 168 PM_(10)samples were collected during the year of 2005 at eight sites in the city of Wuxi in China.Fifteen chemical elements,three water-soluble ions,total carbon and organic carbon were analyzed.Six source categories were identified and their contributions to ambient PM_(10)in Wuxi were estimated using a nested chemical mass balance method that reduces the effects of colinearity on the chemical mass balance model.In addition,the concentrations of secondary aerosols,such as secondary organic carbon,sulfate and nitrate,were quantified.The spatially averaged PM_(10)was high in the spring and winter(123μg·m^(-3)and low in the summer-fall(90μg·m^(-3)).According to the result of source apportionment,resuspended dust was the largest contributor to ambient PM_(10),accounting for more than 50%of the PM_(10)mass.Coal combustion(14.6%)and vehicle exhaust(9.4%)were also significant source categories of ambient PM_(10).Construction and cement dust,sulfates,secondary organic carbon,and nitrates made contributions ranging between 4.1%and 4.9%.Other source categories such as steel manufacturing dust and soil dust made low contributions to ambient PM_(10). | Bo HAN Xiaohui BI Yonghua XUE Jianhui WU Tan ZHU Baogui ZHANG Jianqing DING Yuanxin DU | 2011 | Frontiers of Environmental Science & Engineering2011，5，4： | 5 |
| 14 | WHEAT STRIPE RUST AND INTEGRATION OF SUSTAINABLE CONTROL STRATEGIES IN CHINA显示文摘Stripe (yellow) rust caused by Puccinia striiformis f. sp. tritici occurs in almost allwheat-producing regions of the world. Severe countrywide epidemics in Chinahave caused substantial yield losses. Growing resistant cultivars is the beststrategy to control this disease but the pathogen can overcome resistance inwheat cultivars. The high variation in the virulence of the pathogen combinedwith the large areas of susceptible wheat cultivars enables the pathogenpopulation to increase rapidly and disperse over long distances under favorableenvironmental conditions, resulting in severe pandemics within croppingseasons. Current stripe rust control measures are based on many years ofresearch including the underlying epidemiology regarding year-to-year survivalof the pathogen, pathways of pathogen dispersal within seasons and years, therole of P. striiformis sexual hybridization, the use of resistance sources inbreeding programs, and year-round surveillance of national wheat crops that arepresent in different parts of the country throughout the year. All these strategiesdepend on accurate prediction of epidemics, more precise use of fungicides tomeet national requirements and better deployment of resistance genes. Newideas with potential application in sustainable protection of stripe rust includenegative regulatory gene editing, resistance gene overexpression and biologicalcontrol based on microbiomes. | Qingdong ZENG Jie ZHAO Jianhui WU Gangming ZHAN Dejun HAN Zhensheng KANG | 2022 | Frontiers of Agricultural Science and Engineering2022，9，1： | 5 |
| 15 | Study on the performance of TEG with heat transfer enhancement using graphene-water nanofluid for a TEG cooling system显示文摘Improvement of the heat transfer effect of cold side of a thermoelectric generator(TEG) is one of the approaches to enhance the performance of the TEG systems.As a new type of heat transfer media,nanofluids can enhance the heat transfer performance of working liquid significantly.In this study,the performance of a commercial TEG with graphene-water(GW) nanofluid as coolants in a minichannel heat exchanger is investigated experimentally at low temperatures.The results show that the output power of TEG increases with the flow rate under 950 mL/min.However,the fluid flow rate has no influence on the output power of TEG with higher flow rate(larger than 950 mL/min) when the heat transfer dynamic balance state of the system is reached.The optimal concentration and flow rate of nanofluid are 0.1 wt%and 950 mL/min,respectively.At the optimal conditions,the improved voltage,output power and conversion efficiency with GW nanofluid applied in the cooling system are increased by11.29%,21.55%and 3.5%in comparison with those with only water applied,respectively. | LI YiHuai WU ZiHua XIE HuaQing XING JiaoJiao MAO JianHui WANG Yuan Yuan LI Zhen | 2017 | Science China(Technological Sciences)2017，60，8： | 5 |
| 16 | Vertical characteristics and source identification of PM_(10) in Tianjin显示文摘Ambient PM 10 (particulate matter with a diameter less than 10μm) concentrations were measured on a 255 meter tower in Tianjin,China.The samples were collected at four vertical levels (10,40,120 and 220 m).Vertical characteristics for PM 10 samples were studied.The results showed that the concentrations of PM 10 and constituent species had a negative correlation with the sampling height.The highest concentrations of PM 10 and species were obtained at the 10 m level,and the lowest concentrations were measured at the 220 m level.For the fractions of species to total mass,SO 4 2- and NO 3- had higher values (fraction) at greater height;while Ca had a higher fraction at lower height.Possible source categories for the PM 10 ambient dataset were identified by the principal component analysis method.The possible source categories included crustal dust,vehicles,cement dust,and incineration as well as secondary sulfate and nitrate sources.Analysis of meteorological factors on PM 10 concentrations indicated that wind speed and inversion may be the main factors contributing to different concentrations of PM 10 at different heights. | Zhimei Xiao Jianhui Wu Suqin Han Yufen Zhang Hong Xu Xiaoyong Zhang Guoliang Shi Yinchang Feng | 2012 | Journal of Environmental Sciences2012，24，1： | 5 |
| 17 | Reverse immune suppressive microenvironment in tumor draining lymph nodes to enhance anti-PD1 immunotherapy via nanovaccine complexed microneedle显示文摘The maturation of dendritic cells(DCs)and infiltration effector T cells in tumor-draining lymph node(tdLN)and tumor tissue are crucial for immunotherapy.Despite constructive progresses have been made with anti-programmed death-1(anti-PD1)checkpoint blockade for immunotherapy,the efficacy of PD1/PD-L1 therapy deserves to be improved.Here,we constructed a novel transfersomes based nanovaccine complexed microneedles to enhance anti-PD1 immunotherapy via transdermal immunization for skin tumor therapy.Transfersomes were functionalized with DCs targeting moietyαCD40,co-encapsulated with antigens and adjuvant poly I:C.Moreover,transdermal administration promoted accumulation in tumor-draining lymph nodes(tdLN),which could facilitate cellular uptake,activate DCs maturation and enhance Th1 immune responses.Using a mouse melanoma model,combined therapy of such nanovaccine complexed microneedles with pembrolizumab(αPD1)was able to enhance cytotoxic T lymphocytes activation,promote infiltration and reduce regulatory T cells frequency in tdLN and tumor tissues,which achieved reversion of the immunosuppressive microenvironment into immune activation.This study highlighted the potential of transfersomes based nanovaccines complexed microneedles as an attractive platform for tumor immunotherapy. | Zhongzheng Zhou Jianhui Pang Xuanjin Wu Wei Wu Xiguang Chen Ming Kong | 2020 | Nano Research2020，13，6： | 5 |
| 18 | Haplotype variations in QTL for salt tolerance in Chinese wheat accessions identified by marker-based and pedigree-based kinship analyses显示文摘Most modern wheat cultivars were selected on the basis of yield-related indices measured under optimal fertilizer and irrigation inputs.With climate change,land degradation and salinity caused by sea water encroachment,wheat is increasingly subjected to environmental stress.Moreover,expanding urbanization increasingly encroaches upon prime agricultural land in countries like China,and alternative cropping areas must be found.Some of these areas have moderate constraining factors,such as salinity.Therefore,it is important to investigate whether current genetic materials and breeding procedures are maintaining adequate variability to address future problems caused by abiotic stress.In this study,a panel of 307 wheat accessions,including local landraces,exotic cultivars used in Chinese breeding programs and Chinese cultivars released during different periods since1940,were subjected to a genome-wide association study to dissect the genetic basis of salinity tolerance.Both marker-based and pedigree-based kinship analyses revealed that favorable haplotypes were introduced in some exotic cultivars as well as a limited number of Chinese landraces from the 1940 s.However,improvements in salinity tolerance during modern breeding are not as obvious as that of yield.To broaden genetic diversity for increasing salt tolerance,there is a need to refocus attention on local landraces that have high degrees of salinity tolerance and carry rare favorable alleles that have not been exploited in breeding. | Shizhou Yu Jianhui Wu Meng Wang Weiming Shi Guangmin Xia Jizeng Jia Zhensheng Kang Dejun Han | 2020 | The Crop Journal2020，8，6： | 4 |
| 19 | Homogeneous label-free fluorescent assay of small molecule-protein interactions using protein binding-inhibited transcription nanomachine显示文摘Quantitative analysis of interactions between small molecules and proteins is a central challenge in chemical genetics, molecular diagnostics and drug developments. Here, we developed a RNA transcription nanomachine by assembling T7 RNA polymerase on a small molecule-labeled DNA heteroduplex. The nanomachine, of which the RNA transcription activity can be quantitatively inhibited by protein binding, showed a great potential for small molecule-protein interaction assay. This finding enabled us to develop a novel homogeneous label-free strategy for assays of interactions between small molecules and their protein receptors. Three small molecule compounds and their protein receptors have been used to demonstrate the developed strategy. The results revealed that the protein-small molecule interaction assay strategy shows dynamic responses in the concentration range from 0.5 to 64 nM with a detection limit of 0.2 nM. Due to its label-free, homogeneous, and fluorescence-based detection format, besides its desirable sensitivity this technique could be greatly robust, cost-efficient and readily automated, implying that the developed small molecule-protein interaction assay strategy might create a new methodology for developing intrinsically robust, sensitive and selective platforms for homogeneous protein detection. | ZHOU DianMing, WU YiDan, LIU Pei, BAI HaoTian, TANG LiJuan, YU RuQin & JIANG JianHui State Key Laboratory of Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China | 2011 | Science China Chemistry2011，54，8： | 4 |
| 20 | A grid-based clustering algorithm for wild bird distribution显示文摘 | Yuwei WANG Yuanchun ZHOU Ying LIU Ze LUO Danhuai GUO Jing SHAO Fei TAN Liang WU Jianhui LI Baoping YAN | 2013 | Frontiers of Computer Science2013，7，4： | 4 |
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