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| 1 | H. pylori Escape Host Immunoreaction Through Inhibiting ILK Expression by VacA显示文摘Helicobacter pylori (H。pylori ) 尽管有精力旺盛的有免疫力的回答,固执地开拓殖民地于胃的 mucosa。Vacuolating 细胞毒素由 H 藏匿了。pylori 结果是有势力 immunomodulatory 毒素,而是包含的信号 transduction 小径没在巨噬细胞被学习。我们在这研究观察了那 vacA 缺乏的 H。pylori 导致了连接 integrin 的 kinase (同类) 和 endothelial 的显著地更高的表示氮的氧 synthase (eNOS ) ,并且显著地在 monocyte/macrophage-like U937 房间的反应的氧种类(ROS ) 的更多的生产,同样与 isogenic vacA+H 相比。pylori。在 U937 房间 overexpressing 同类的 eNOS mRNA 的表示显著地与空向量与那些 transfected 相比被增加。因此, vacA 缺乏的 H。pylori 出现到 upregulate 同类表示,调制 eNOS 并且作为结果的表示,刺激 ROS 的生产。,帮助 H 是 VacA 由禁止同类表示阻止如此的进程。pylori 逃跑主人免疫反应。这机制解释,至少部分地, H 的坚持的感染。在胃的 pylori。 | Jianping Yuan Ping Li Jing Tao Xiaodong Shi Baoyu Hu Huabiao Chen Xiaokui Guo | 2009 | Cellular & Molecular Immunology2009，6，3： | 12 |
| 2 | Structure of the organic crystallite unit in coal as determined by X-ray diffraction显示文摘X-ray diffraction (XRD) was used to study the structure of the organic crystallite unit (La, Lc, d002) in coals collected from Henan and Shanxi Provinces. XRD patterns of coal were collected in a step-scan mode (0.1°/step) over an angular range of 2-90° (2h), allowing 8 s at each step. The structure of the crystallite unit was determined from the Scherrer equation and peak parameters deduced from whole pattern fit- ting. The results show that the structure of the crystallite unit in coal is mainly controlled by the coal rank. As the coal rank increases the average diameter of a coal crystallite unit (La) increases, the inter- layer spacing (d002) decreases slightly, and the average height of a coal crystallite unit (Lc) increases at first but then decreases. A new diffraction peak from the crystallite unit in coal was found at a low scat- tering angle in the XRD pattern (2-10°). This suggests a structure with an inter-layer spacing from 1.9 to 2.8 nm exists in coal crystallites. | Song Dangyu Yang Cunbei Zhang Xiaokui Su Xianbo Zhang Xiaodong | 2011 | Mining Science and Technology2011，21，5： | 12 |
| 3 | Review of control and guidance technology on hypersonic vehicle显示文摘Air-breathing hypersonic vehicle has great military and potential economic value due to its characteristics:high velocity,long range,quick response.Therefore,the development of hypersonic vehicle and its guidance and control technology are reviewed in this paper.Firstly,the development and classification of hypersonic vehicles around the world are summarized,and the geometric configuration and mission profile of typical air-breathing hypersonic vehicle are given.Secondly,the control difficulties of air-breathing hypersonic vehicle are introduced,including integrated design of engine and fuselage,static instability,strong nonlinearity,uncertain aerodynamic parameters,etc.According to its control requirements,the control methods considering external disturbance,fault-tolerant control methods,anti-saturation methods,and prescribed performance control methods considering transient performance constraints are summarized respectively.The classification and comparison of various control methods are given,and the frontiers of theoretical development are analyzed.Finally,considering the effects of composite disturbances,the design of terminal guidance law under multiple constraints is overviewed,including guidance law with angle constraint,velocity constraint,acceleration constraint and time constraint.Similarly,the classification of guidance law design methods under different constraints,their advantages as well as the future development trend and requirements are introduced. | Yibo DING Xiaokui YUE Guangshan CHEN Jiashun SI | 2022 | Chinese Journal of Aeronautics2022，35，7： | 9 |
| 4 | Analysis of the microbiota of sputum samples from patients with lower respiratory tract infections显示文摘唾沫是从患更低的呼吸的道感染的病人收集的最普通的样品,它为这些感染的细菌的鉴定是关键的。在这研究,我们与更低的呼吸的道感染从 101 个病人注册了 101 件唾沫样品。开始,在唾沫包含的细菌的 16S rDNA V3 hypervariable 区域的 pyrosequencing 为 microbiota 分析作为一条文化无关的途径被利用。为比较,用一个文化依赖者的临床的实验室测试为样品也被做的唾沫的一样的队自动化了细菌的鉴定系统。由 pyrosequencing, > 70,000 DNA 碎片在被 Ribosomal 数据库工程(RDP ) 过程分析以后被发现并且分类进 129 个细菌的类。大多数序列属于几个占优势的类,例如链球菌和葡萄球菌,显示这些类起在更低的呼吸的道感染的一个重要作用。另外,属于潜在的原因的代理人的一些序列例如霉浆菌, Haemophilus,和 Moraxella,也被发现,但是这些序列没被临床的实验室测试发现。为两个方法检测的九个类,方法的敏感被比较,结果证明 pyrosequencing 是更敏感的,除了 Klebsiella 和 Mycobacterium。显著地,这个方法揭示了更复杂的细菌的社区,它为细菌的察觉显示出一个有希望的能力。 | Yuhua Zhou Ping Lin Qingtian Li Lizhong Han Huajun Zheng Yanxia Wei Zelin Cui Yuxing Ni Xiaokui Guo | 2010 | Acta Biochimica et Biophysica Sinica2010，42，10： | 8 |
| 5 | Selective leaching of vanadium from V-Ti magnetite concentrates by pellet calcification roasting-H_(2)SO_(4) leaching process显示文摘A novel method of pellet calcification roasting-H_(2)SO_(4) leaching was proposed to efficiently separate and extract vanadium(V)from vanadium-titanium(V-Ti)magnetite concentrates.The leaching rate of V is as high as 88.98%,while the leaching rate of impurity iron is only 1.79%.Moreover,the leached pellets can be used as raw materials for blast furnace ironmaking after secondary roasting.X-ray photoelectron spectroscopy(XPS)and scanning electron microscopy with energy dispersive X-ray spectrometry(SEMEDS)analyses showed that V^(3+)was oxidized to V^(5+)after roasting at 1200℃,and V^(5+)was then leached by H_(2)SO_(4).X-ray diffraction(XRD)analyses and single factor experiment revealed a minimal amount of dissolved Fe_(2)O_(3) during H_(2)SO_(4) leaching.Therefore,a high separation degree of V and iron(Fe)from V-Ti magnetite concentrate was achieved through H_(2)SO_(4) leaching.Compared with the traditional roastingleaching process,this process can achieve a high selectivity of V and Fe,and has excellent prospects for industrial production. | Yi Luo Xiaokui Che Xinglan Cui Qi Zheng Lei Wang | 2021 | International Journal of Mining Science and Technology2021，31，3： | 8 |
| 6 | Microwave assisted atmospheric acid leaching of nickel from laterite ore显示文摘The recovery of nickel from laterite ore with sulphuric acid under the effect of microwave irradiation was studied.The experimental results indicated that the extraction rate of nickel was influenced by reaction time,sulphuric acid concentration,and temperature,especially by microwave power.The results obtained from the experiments of orthogonal arrays showed that the optimum conditions of sulphuric acid concentration,reaction time,microwave power,and temperature were 25 vol.%,1.5 h,600 W,and 90°C,respectively.Under the optimal conditions,the nickel recovery could reach approximately 90.8%,which was higher than that obtained by conventional water bath heating.Kinetic experiments showed that the leaching of nickel in a sulphuric acid medium was controlled by chemical reaction occurring on the surface of laterite ore.The apparent activation energy was 38.9 kJ/mol.Microwave heating technology is efficient,clean,and easy to control and facilitate continuous processing of materials. | CHE Xiaokui SU Xiuzhu CHI Ru'an YU Junxia | 2010 | Rare Metals2010，29，3： | 7 |
| 7 | Protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium on ovarian damage显示文摘Chemotherapeutic agents are extensively used to treat malignancies.However,chemotherapy-induced ovarian damage and reduced fertility are severe side effects.Recently,stem cell transplantation has been reported to be an effective strategy for premature ovarian insufficiency(POI)treatment,but safety can still be an issue in stem cell-based therapy.Here,we show the protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium(hUCMSC-CM)on a cisplatin(Cs)-induced ovarian injury model.hUCMSC-CM can relieve Cs-induced depletion of follicles and preserve fertility.In addition,hUCMSC-CM can decrease apoptosis of oocytes and granulosa cells induced by Cs.RNA sequencing analysis reveals the differentially expressed genes of ovaries after Cs and hUCMSC-CM treatments,including genes involved in cell apoptosis.Furthermore,we show that the granulocyte colony-stimulating factor(G-CSF)/phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)pathway plays an important role in protecting granulosa cells from Cs-induced apoptosis.Together,we confirm the protective effects of hUCMSC-CM on ovarian reserve and fertility in mice treated with Cs,highlighting the remarkable therapeutic effects of hUCMSC-CM. | Liming Hong Long Yan Zhimin Xin Jie Hao Wenjing Liu Shuyu Wang Shujie Liao Hongmei Wang Xiaokui Yang | 2020 | Journal of Molecular Cell Biology2020，12，5： | 7 |
| 8 | Adaptive control for attitude coordination of leader-following rigid spacecraft systems with inertia parameter uncertainties显示文摘This paper studies the leader-following attitude coordination problems of multiple spacecraft in the presence of inertia parameter uncertainties. To achieve attitude coordination in the situation that even the leader's attitude is only applicable to a part of the following spacecraft, a nonlinear attitude observer is proposed to obtain an accurate estimation of the leader's attitude and angular velocity for all the followers. In addition, a distributed control scheme based on noncertainty equivalence principle is presented for multiple spacecraft' attitude synchronization. With a dynamic scaling, attitude consensus can be achieved asymptotically without any information of the bounds of the uncertain inertia parameters. Furthermore, once the estimations of inertia parameters reach their ideal values, the estimation process will stop and the ideal value of inertia parameter will be held. This is a special advantage of parameter estimation method based on non-certainty equivalence. Numerical simulations are presented to demonstrate that the proposed non-certainty equivalence-based method requires smaller control toque and converges faster compared with the certainty equivalence-based method. | Xiaokui YUE Xianghong XUE Haowei WEN Jianping YUAN | 2019 | Chinese Journal of Aeronautics2019，32，3： | 6 |
| 9 | Cloning and characterization of three cheB genes in Leptospira interrogans显示文摘活动性和趋化性系统为 leptospires 的毒力是批评的。有在 leptospires 定位的通常认为的趋化性相当或相同的事物的多重拷贝大染色体。从螺旋体 interrogans 紧张 Lai 的 CheB1 和 CheB3 被预言有一个全球象 CheB 一样领域,但是 CheB2 被预言仅仅有一个 C 终端受动器领域。以便验证三通常认为的 cheB 基因的功能,他们被克隆进 pQE31 向量然后分别地,在野类型的 Escherichia coli 紧张 RP437 和 cheB 有缺陷者紧张 RP4972 表示了。聚集的试金的结果和 CheB1 的预言的第三的结构和 L 的 CheB3。interrogans 紧张 Lai 建议一个 N 终端规章的领域的缺席可以是原因之一让 CheB2 的失败补充 E。coli cheB 异种。而且, CheB2 在 leptospires 的相互作用网络完全连接到 CheR1 和 CheR3。一起拿,这些结果显示 CheB2 不能独自工作,并且在某些生理的条件下面,工作可以要求 CheB3 和 CheR1。趋化性基因相当或相同的事物的多重拷贝的存在建议了那 L。interrogans 紧张 Lai 可能有一条更复杂的化学感应的小径。 | Ke Dong Qingtian Li Chang Liu Yunyi Zhang Guoping Zhao Xiaokui Guo | 2010 | Acta Biochimica et Biophysica Sinica2010，42，3： | 5 |
| 10 | Cilastatin protects against imipenem-induced nephrotoxicity via inhibition of renal organic anion transporters(OATs)显示文摘Imipenem is a carbapenem antibiotic. However, Imipenem could not be marketed owing to its instability and nephrotoxicity until cilastatin, an inhibitor of renal dehydropeptidase-I(DHP-I),was developed. In present study, the potential roles of renal organic anion transporters(OATs) in alleviating the nephrotoxicity of imipenem by cilastatin were investigated in vitro and in rabbits. Our results indicated that imipenem and cilastatin were substrates of h OAT1 and h OAT3. Cilastatin inhibited h OAT1/3-mediated transport of imipenem with IC50 values comparable to the clinical concentration, suggesting the potential to cause a clinical drug–drug interaction(DDI). Moreover,imipenem exhibited h OAT1/3-dependent cytotoxicity, which was alleviated by cilastatin and probenecid. Furthermore, cilastatin and probenecid ameliorated imipenem-induced rabbit acute kidney injury, and reduced the renal secretion of imipenem. Cilastatin and probenecid inhibited intracellular accumulation of imipenem and sequentially decreased the nephrocyte toxicity in rabbit primary proximal tubule cells. Renal OATs, besides DHP-I, was also the target of interaction between imipenem and cilastatin, and contributed to the nephrotoxicity of imipenem. This therefore gives in part the explanation about the mechanism by which cilastatin protected against imipenem-induced nephrotoxicity. Thus, OATs can potentially be used as a therapeutic target to avoid the renal adverse reaction of imipenem in clinic. | Xiaokui Huo Qiang Meng Changyuan Wang Yanna Zhu Zhihao Liu Xiaodong Ma Xiaochi Ma Jinyong Peng Huijun Sun Kexin Liu | 2019 | Acta Pharmaceutica Sinica B2019，9，5： | 5 |
| 11 | Correlation analysis between the chemical contents and bioactivity for the quality control of Alismatis Rhizoma显示文摘In order to clarify regions of production and to discriminate processing methods, quantitative and qualitative analyses for saccharides and terpenes in 35 batches of Alismatis Rhizoma were performed.Methodologies included HPLC–PDA, HPLC–VWD and UHPLC–MSn, combined with principal component analysis(PCA) and partial least squares regression techniques(PLSR). The inhibitory effects of triterpenes and Alismatis Rhizoma extracts on lipase activity were evaluated in vitro. PLSR analysis revealed significant positive correlations(R2= 0.5795) between the contents of triterpenes 10, 14, 15, 18 and 22 and the inhibitory effects of Alismatis Rhizoma. The present study establishes an effective method for simultaneous determination of multiple components, and identifies key bioactive triterpenes. These results can be used for systematic and novel analytical strategies for the quality control of Alismatis Rhizoma production. | Xiaoxv Gao Chengpeng Sun Zhenglong Yu Jian Cang Xiangge Tian Xiaokui Huo Lei Feng Xinguang Liu Chao Wang Baojing Zhang Xiaochi Ma | 2018 | Acta Pharmaceutica Sinica B2018，8，2： | 4 |
| 12 | Postcapture stabilization of space robots considering actuator failures with bounded torques显示文摘Space robotics is regarded as one of the most impressing approaches for space debris removal missions. Due to the residual momentum of debris, it is essential to stabilize the base rapidly after capture. This paper presents a novel control strategy for stabilization of a space robot in postcapture considering actuator failures and bounded torques. In the control strategy, the motion of the manipulator is not regarded as a disturbance to the base; in contrast, it is utilized to compensate for the limitation of the control torques by means of an inverse dynamical model of the system. Different scenarios where actuators are external mechanisms or momentum exchange devices have been carried out, and for actuator failures, both single-and two-actuator failures have been considered. Regarding to the performance of actuators, control torques are bounded. In cases that either single or two actuators have failed, the base can be stabilized kinematically when actuators are external mechanisms, but can only be stabilized dynamically when only momentum exchange devices are used. Finally, a space robot with a seven-degree-of-freedom manipulator in postcapture is studied to verify the validity and feasibility of the proposed control scheme. Simulation results show that the whole system can be stabilized rapidly. | Xiaokui YUE Teng ZHANG Honghua DAI Xin NING Jianping YUAN | 2018 | Chinese Journal of Aeronautics2018，31，10： | 4 |
| 13 | Prediction and systematic study of protein-protein interaction networks of Leptospira interrogans显示文摘Leptospira interrogans serovar Lai is a pathogenic bacterium that causes a spirochetal zoonosis in humans and some animals. With its complete genome sequence available, it is possible to analyze protein-protein interactions from a whole- genome standpoint. Here we combine four recently developed computational approaches (gene fusion method, gene neighbor method, phylogenetic profiles method, and operon method) to predict protein-pro- tein interaction networks of Leptospira interrogans strain Lai. Through comprehensive analysis on in- teractions among proteins of motility and chemotaxis system, signal transduction, lipopolysaccaride bio- synthesis and a series of proteins related to adhesion and invasion, we provided information for further studying on its pathogenic mechanism. In addition, we also assigned 203 previously uncharacterized proteins with possible functions based on the known functions of its interacting partners. This work is helpful for further investigating L. interrogans strain Lai. | SUN Jingchun XU Jinlin CAO Jianping LIU Qi GUO Xiaokui SHI Tieliu LI Yixue | 2006 | Chinese Science Bulletin2006，51，11： | 3 |
| 14 | Motion estimation in wide band synthetic aperture sonar based on the raw echo data using the method of displaced phase center antenna显示文摘Phase errors in synthetic aperture sonar (SAS) imaging must be reduced to less than one eighth of a wavelength so as to avoid image destruction. Most of the phase errors occur as a result of platform motion errors, for example, sway yaw and surge that are the most important error sources. The phase error of a wide band synthetic aperture sonar is modeled and solutions to sway yaw and surge motion estimation based on the raw sonar echo data with a Displaced Phase Center Antenna (DPCA) method are proposed and their implementations are detailed in this paper. It is shown that the sway estimates can be obtained from the correlation lag and phase difference between the returns at coincident phase centers. An estimate of yaw is also possible if such a technique is applied to more than one overlapping phase center positions. Surge estimates can be obtained by identifying pairs of phase centers with a maximum correlation coefficient. The method works only if the platform velocity is low enough such that a number of phase centers from adjacent pings overlap. | JIANG Xiaokui, SUN Chao (Member, IEEE), FANG Jie (Institute of Acoustic Engineering, Northwestern Polytechnical University, Xi’an 710072, China) | 2003 | 声学技术2003，22，z1： | 3 |
| 15 | New microbiota found in sputum from patients with community-acquired pneumonia显示文摘获得社区的肺病(帽子) 是医院和哪个还全身地没被讨论的细菌的社区里的一颗主要担心。从在尖锐阶段的病人的唾沫是反映它的特征的一种可存取的样品。在我们的学习,我们与帽子从 45 个病人分析了 45 件唾沫样品。从健康的人的十八件唾沫样品被选择为控制。在唾沫包含的所有细菌的 16 年代 rDNA V3 hypervariable 区域的 Pyrosequencing 被用作一个文化无关的方法揭示社区体质。另外,我们为在唾沫的获得医院的肺病(幸运) 的出版数据被用于比较。由 pyrosequencing, > 90,000 DNA 读被检测。在被工具在 Ribosomal 数据库工程分析以后, reads 被分类进五个主要的数并且 > 100 个类。在数水平,读的帽子的分发类似于健康的人并且在类水平的,每个类的出现在三个范畴拥有他们的特征。象链球菌和 Neisseria 那样的类在他们的百分比显示出稳定性,显示如此的类被外长的细菌或抗菌素很少影响。象在帽子的 Moraxella 和 Rothia 那样的另外的类的角色应该被强调。根据我们的分析,当时,帽子的细菌的社区与细微变化与那些相比健康的人,但是有在幸运之间的大差距。同时, Rothia 可能是一个重要内长的引起肺病的因素。 | Chunyan Chen Tian Shen Fei Tian Ping Lin Qingtian Li Zelin Cui Yajuan Zhang Min Xue Jing Ye Xiaokui Guo YuhuaZhou | 2013 | Acta Biochimica et Biophysica Sinica2013，45，12： | 3 |
| 16 | Organic anion transporters also mediate the drug–drug interaction between imipenem and cilastatin显示文摘This study aimed to clarify that organic anion transporters(OATs)mediate the drug–drug interaction(DDI)between imipenem and cilastatin.After co-administration with imipenem,the plasma concentrations and the plasma concentration-time curve(AUC)of cilastatin were significantly increased,while renal clearance and cumulative urinary excretion of cilastatin were decreased.At the same time,imipenem significantly inhibited the uptake of cilastatin in rat kidney slices and in human OAT1(hOAT1)-HEK293 and human OAT3(hOAT3)-HEK293 cells.Probenecid,p-aminohippurate,and benzylpenicillin inhibited the uptake of imipenem and cilastatin in rat kidney slices and in hOAT1-and hOAT3-HEK 293 cells,respectively.The uptakes of imipenem and cilastatin in hOAT1-and hOAT3-HEK 293 cells were significantly higher than that in mock-HEK-293 cells.Moreover,the K m values of cilastatin were increased in the presence of imipenem with unchanged V max,indicating that imipenem inhibited the uptake of cilastatin in a competitive manner.When imipenem and cilastatin were co-administered,the level of imipenem was higher compared with imipenem alone both in vivo and in vitro.But,cilastatin significantly inhibited the uptake of imipenem when dehydropeptidase-1(DPEP1)was silenced by RNAi technology in hOAT1-and hOAT3-HEK 293 cells.In conclusion,imipenem and cilastatin are the substrates of OAT1 and OAT3.OAT1 and OAT3 mediate the DDI between imipenem and cilastatin.Meanwhile,cilastatin also reduces the hydrolysis of imipenem by inhibiting the uptake of imipenem mediated by OAT1 and OAT3 in the kidney as a complement. | Yanna Zhu Xiaokui Huo Changyuan Wang Qiang Meng Zhihao Liu Huijun Sun Aiping Tan Xiaodong Ma Jinyong Peng Kexin Liu | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，2： | 3 |
| 17 | 三维重建组织多普勒成像测量心肌梗死面积的实验研究显示文摘目的 通过活体动物实验探讨三维重建组织多普勒超声成像测量心肌梗死面积的方法和可行性。方法 用三维重建组织多普勒显像的方法 ,对 8只开胸的心尖部心肌梗死和室壁瘤的活体羊模型在 4种不同的血流状态下进行心肌梗死面积的测量。结果 重建三维超声组织多普勒成像测量的活体羊心内膜梗死的面积与尸检标本测量的面积密切相关 (r =0 .90 ,Y =0 .83X +1 .33 ,s x =0 .92cm2 ,P <0 .0 0 0 1 )。结论 重建三维组织多普勒成像提供了一系列动态空间组织多普勒数据确定室壁异常运动区 ,并能定量测量心肌梗死面积 ,在缺血性心脏疾病中 。 | 王慧芳 Xiaokui Li David J.Sahn Michael Jones Crispin H.Davies Rosemary A.Rusk Arthur D.Zetts | 2002 | 中华超声影像学杂志2002，11，11： | 3 |
| 18 | A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living cells and tissues显示文摘Uridine diphosphate(UDP)-glucuronosyltransferases(UGTs)are enzymes involved in the biotransformation of important endogenous compounds such as steroids,bile acids,and hormones as well as exogenous substances including drugs,environmental toxicants,and carcinogens.Here,a novel fluorescent probe BDMP was developed based on boron-dipyrromethene(BODIPY)with high sensitivity for the detection of UGT1A8.The glucuronidation of BDMP not only exhibited a redemission wavelength(lex/lem=500/580 nm),but also displayed an excellent UGT1A8-dependent fluorescence signal with a good linear relationship with UGT1A8 concentration.Based on this perfect biocompatibility and cell permeability,BDMP was successfully used to image endogenous UGT1A8 in human cancer cell lines(LoVo and HCT15)in real time.In addition,BDMP could also be used to visualize UGT1A8 in tumor tissues.These results suggested that BDMP is a promising molecular tool for the investigation of UGT1A8-mediated physiological function in humans. | Mingyue Zhu Zhenhao Tian Lingling Jin Xiaokui Huo Chao Wang Jingnan Cui Yan Tian Xiangge Tian Lei Feng | 2022 | Frontiers of Chemical Science and Engineering2022，16，1： | 3 |
| 19 | Arenobufagin is a novel isoform-specific probe for sensing human sulfotransferase 2A1显示文摘Human cytosolic sulfotransferase 2 A1(SULT2A1) is an important phase II metabolic enzyme. The detection of SULT2A1 is helpful for the functional characterization of SULT2A1 and diagnosis of its related diseases. However, due to the overlapping substrate specificity among members of the sulfotransferase family, it is difficult to develop a probe substrate for selective detection of SULT2A1. In the present study, through characterization of the sulfation of series of bufadienolides, arenobufagin(AB) was proved as a potential probe substrate for SULT2A1 with high sensitivity and specificity. Subsequently, the sulfation of AB was characterized by experimental and molecular docking studies. The sulfate-conjugated metabolite was identified as AB-3-sulfate.The sulfation of AB displayed a high selectivity for SULT2A1 which was confirmed by in vitro reaction phenotyping assays. The sulfation of AB by human liver cytosols and recombinant SULT2A1 both obeyed Michaelis-Menten kinetics, with similar kinetic parameters. Molecular docking was performed to understand the interaction between AB and SULT2A1, in which the lack of interaction with Met-137 and Tyr-238 of SULT2A1 made it possible to eliminate substrate inhibition of AB sulfation. Finally, the probe was successfully used to determine the activity of SULT2A1 and its isoenzymes in tissue preparations of human and laboratory animals. | Xiangge Tian Chao Wang Peipei Dong Yue An Xinyu Zhao Weiru Jiang Gang Wang Jie Hou Lei Feng Yan Wang Guangbo Ge Xiaokui Huo Jing Ning Xiaochi Ma | 2018 | Acta Pharmaceutica Sinica B2018，8，5： | 2 |
| 20 | Kinetics, thermodynamics, and equilibrium of As(Ⅲ),Cd(Ⅱ), Cu(Ⅱ) and Pb(Ⅱ) adsorption using porous chitosan bead-supported MnFe_(2)O_(4) nanoparticles显示文摘A novel porous nanocomposite,cross-linked chitosan and polyethylene glycol(PEG) bead-supported MnFe_(2) O_(4) nanoparticles(CPM),was developed as an efficient adsorbent to remove metalloid(As(Ⅲ))and heavy metals(Cd(Ⅱ),Cu(Ⅱ),and Pb(Ⅱ)).The characteristics of CPM showed a porous structure,well dispersed MnFe_(2) O_(4),and several of hydroxyl and amino groups(-OH,-NH_(2)).Batch experiments demonstrated that the best adsorption property of As(Ⅲ),Cd(Ⅱ),Cu(Ⅱ),and Pb(Ⅱ) was achieved within 8 h with maximum adsorption capacities of 9.90,9.73,43.94,and 11.98 mg/g,respectively.Competitive and synergistic effects(particularly precipitation) were included in the co-adsorption mechanism of As(Ⅲ) and heavy metals.Thereinto,As(Ⅲ) was partly oxidized by MnFe_(2) O_(4) to As(V),and both were coordinated on MnFe_(2) O_(4) nanoparticles.Pb(Ⅱ) could also bind to MnFe_(2) O_(4) by ion exchange and electrostatic attraction.Furthermore,Cd(Ⅱ) and Cu(Ⅱ) tended to be coordinated on chitosan.Therefore,CPM can serve as a remediation material for water and soil co-contaminated with As(Ⅲ) and heavy metals. | Hongxia Li Hongbing Ji Xinglan Cui Xiaokui Che Qidong Zhang Juan Zhong Rongzhen Jin Lei Wang Yi Luo | 2021 | International Journal of Mining Science and Technology2021，31，6： | 2 |
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