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1GS6, A Member of the GRAS Gene Family,Negatively Regulates Grain Size in Rice显示文摘Grain size is an important yield related trait in rice.Intensive arti cial selection for grain size during domestication is evidenced by the larger grains of most of today’s cultivars compared with their wild relatives.However,the molecular genetic control of rice grain size is still not well characterized.Here,we report the identi cation and cloning of Grain Size 6(GS6),which plays an important role in reducing grain size in rice.A premature stop at the t348 position in the coding sequence(CDS)of GS6 increased grain width and weight signi cantly.Alignment of the CDS regions of GS6 in 90 rice materials revealed three GS6alleles.Most japonica varieties(95%)harbor the Type I haplotype,and 62.9%of indica varieties harbor the Type II haplotype.Association analysis revealed that the Type I haplotype tends to increase the width and weight of grains more than either of the Type II or Type III haplotypes.Further investigation of genetic diversity and the evolutionary mechanisms of GS6showed that the GS6gene was strongly selected in japonica cultivars.In addition,a'ggc'repeat region identi ed in the region that encodes the GRAS domain of GS6 played an important historic role in the domestication of grain size in rice.Knowledge of the function of GS6might aid efforts to elucidate the molecular mechanisms that control grain development and evolution in rice plants,and could facilitate the genetic improvement of rice yield.Lianjun Sun Xiaojiao Li Yongcai Fu Zuofeng Zhu Lubin Tan Fengxia Liu Xianyou Sun Xuewen Sun Chuanqing Sun 2013Journal of Integrative Plant Biology2013,55,10:44
2LHD1,an Allele of DTH8/Ghd8,Controls Late Heading Date in Common Wild Rice (Oryza rufipogon)显示文摘Flowering at suitable time is very important for plants to adapt to complicated environments and produce their seeds successfully for reproduction.In rice (Oryza rufipogon Griff.) photoperiod regulation is one of the important factors for controlling heading date.Common wild rice,the ancestor of cultivated rice,exhibits a late heading date and a more sensitive photoperiodic response than cultivated rice.Here,through map-based cloning,we identified a major quantitative trait loci (QTL) LHD1 (Late Heading Date 1),an allele of DTH8/Ghd8,which controls the late heading date of wild rice and encodes a putative HAP3/NF-YB/CBF-A subunit of the CCAAT-box-binding transcription factor.Sequence analysis revealed that several variants in the coding region of LHD1 were correlated with a late heading date,and a further complementary study successfully rescued the phenotype.These results suggest that a functional site for LHD1 could be among those variants present in the coding region.We also found that LHD1 could down-regulate the expression of several floral transition activators such as Ehd1,Hd3a and RFT1 under long-day conditions,but not under short-day conditions.This indicates that LHD1 may delay flowering by repressing the expression of Ehd1,Hd3a and RFT1 under long-day conditions.Xiaodong Dai Younian Ding Lubin Tan Yongcai Fu Fengxia Liu Zuofeng Zhu Xianyou Sun Xuewen Sun Ping Gu Hongwei Cai Chuanqing Sun 2012Journal of Integrative Plant Biology2012,54,10:23
3Survival of transplanted neurotrophin-3 expressing human neural stem cells and motor function in a rat model of spinal cord injury显示文摘<正>BACKGROUND:Many methods have been attempted to repair nerves following spinal cord injury, including peripheral nerve transplantation,Schwann cell transplantation,olfactory ensheathing cell transplantation,and embryonic neural tissue transplantation.However,there is a need for improved outcomes. OBJECTIVE:To investigate the repair feasibility for rat spinal cord injury using human neural stem cells(hNSCs) genetically modified by lentivirus to express neurotrophin-3. DESIGN,TIME AND SETTING:In vitro cell biological experiment and in vivo randomized,controlled, genetic engineering experiment were performed at the Third Military Medical University of Chinese PLA and First People's Hospital of Yibin,China from March 2006 to December 2007. MATERIALS:A total of 64 adult,female,Wistar rats were used for the in vivo study.Of them,48 rats were used to establish models of spinal cord hemisection,and were subsequently equally and randomly assigned to model,genetically modified hNSC,and normal hNSC groups.The remaining 16 rats served as normal controls. METHODS:hNSCs were in vitro genetically modified by lentivirus to secrete both green fluorescence protein and neurotrophin-3.Neurotrophin-3 expression was measured by Western blot. Genetically modified hNSC or normal hNSC suspension(5×10~5) was injected into the rat spinal cord following T_(10) spinal cord hemisection.A total of 5μL Dulbecco's-modified Eagle's medium was infused into the rat spinal cord in the model group.Transgene expression and survival of transplanted hNSCs were determined by immunohistochemistry.Motor function was evaluated using the Basso,Beattie,and Bresnahan(BBB) scale. MAIN OUTCOME MEASURES:The following parameters were measured:expression of neurotrophin-3 produced by genetically modified hNSCs,transgene expression and survival of hNSCs in rats,motor function in rats. RESULTS:hNSCs were successfully genetically modified by lentivirus to stably express neurotrophin-3.The transplanted hNSCs primarily gathered at,or around,the injection site two weeks following transplantation,and gradually migrated towards the surrounding tissue. Transplanted hNSCs were observed 7.0-8.0 mm away from the injection site.In addition,hNSCs were observed 10 weeks after transplantation.At week 4,BBB locomotor scores were significantly greater in the genetically modified hNSC and normal hNSC groups,compared with the model group (P<0.05),and scores were significantly greater in the genetically modified hNSC group compared with the normal hNSC group(P<0.05). CONCLUSION:hNSCs were genetically modified with lentivirus to stably secrete neurotrophin-3. hNSCs improved motor function recovery in rats following spinal cord injury.Peiqiang Cai Guangyun Sun Peishu Cai Martin Oudega Rui Xiao Xuewen Wang Wei Li Yunbing Shu Cheng Cai Haihao Yang Xuebing Shan Wuhua Luo 2009Neural Regeneration Research2009,4,7:18
4Plasmonic nano-printing: large-area nanoscale energy deposition for efficient surface texturing显示文摘The lossy nature of plasmonic wave due to absorption is shown to become an advantage for scaling-up a large area surface nanotexturing of transparent dielectrics and semiconductors by a self-organized sub-wavelength energy deposition leading to an ablation pattern—ripples—using this plasmonic nano-printing.Irreversible nanoscale modifications are delivered by surface plasmon polariton(SPP)using:(i)fast scan and(ii)cylindrical focusing of femtosecond laser pulses for a high patterning throughput.The mechanism of ripple formation on ZnS dielectric is experimentally proven to occur via surface wave at the substrate–plasma interface.The line focusing increase the ordering quality of ripples and facilitates fabrication over wafer-sized areas within a practical time span.Nanoprinting using SPP is expected to open new applications in photo-catalysis,tribology,and solar light harvesting via localized energy deposition rather scattering used in photonic and sensing applications based on re-scattering of SPP modes into far-field modes.Lei Wang Qi-Dai Chen Xiao-Wen Cao Ričardas Buividas Xuewen Wang Saulius Juodkazis Hong-Bo Sun 2017Light(Science & Applications)2017,6,1:15
5A proposed surface resistance model for the Penman-Monteith formula to estimate evapotranspiration in a solar greenhouse显示文摘Greenhousing is a technique to bridge season gap in vegetable production and has been widely used worldwide. Calculation of water requirement of crops grown in greenhouse and determination of their irrigation schedules in arid and semi-arid regions are essential for greenhouse maintenance and have thus attracted increased attention over the past decades. The most common method used in the literature to estimate crop evapotranspiration(ET) is the Penman-Monteith(PM) formula. When applied to greenhouse, however, it often uses canopy resistance instead of surface resistance. It is understood that the surface resistance in greenhouse is the result of a combined effect of canopy restriction and soil-surface restriction to water vapor flow, and the relative dominance of one restriction over another depends on crop canopy. In this paper, we developed a surface resistance model in a way similar to two parallel resistances in an electrical circuit to account for both restrictions. Also, considering that wind speed in greenhouse is normally rather small, we compared three methods available in the literature to calculate the aerodynamic resistance, which are the r_a^1 method proposed by Perrier(1975a, b), the r_a^2 method proposed by Thom and Oliver(1977), and the r_a^3 method proposed by Zhang and Lemeu(1992). We validated the model against ET of tomatoes in a greenhouse measured from sap flow system combined with micro-lysimeter in 2015 and with weighing lysimeter in 2016. The results showed that the proposed surface resistance model improved the accuracy of the PM model, especially when the leaf area index was low and the greenhouse was being irrigated. We also found that the aerodynamic resistance calculated from the r_a^1 and r_a^3 methods is applicable to the greenhouse although the latter is slightly more accurate than the former. The proposed surface resistance model, together with the r_a^3 method for aerodynamic resistance, offers an improved approach to estimate ET in greenhouse using the PM formula.GONG Xuewen LIU Hao SUN Jingsheng GAO Yang ZHANG Xiaoxian Shiva K JHA ZHANG Hao MA Xiaojian WANG Wanning 2017Journal of Arid Land2017,9,4:12
6Expression of neurocan mRNA and ultrastructure of brain tissue after cerebral ischemia and reperfusion in stroke-prone renovascular hypertensive rats treated by electroacupuncture显示文摘We established a stroke-prone renovascular hypertensive rat model by bilateral constriction of the renal artery with sliver loop clips. After ten weeks, middle cerebral artery occlusion was induced for 2 hours. The rats then received electro-acupuncture at Baihui (DU 20) and Dazhui (DU 14) after onset of ischemia for 30 days. In situ hybridization study showed that electroacupuncture significantly reduced the number of neurocan mRNA-positive cells in the ischemic penumbra and hippocampal tissues of rats. Electron microscopy results demonstrated that the structure of neurons and blood vessels in the ischemic tissues were restored with electroacupuncture. Overall, these data suggest that electroacupuncture may protect neurons against ischemic reperfusion injury in stroke-prone renovascular hypertensive rats, which may be regulated by downregulation of expression of nerve inhibitory factor neurocan mRNA.Feng Tan Saiying Wan Haike Wu Qiwen HUO Jinliang Wang Wenlin Chen Meifeng Fang Xiaolin Liu Xuewen Wang Jingbo Sun 2011Neural Regeneration Research2011,6,36:8
7Alkylation mechanism of benzene with 1-dodecene catalyzed by Et_3NHCl-AlCl_3显示文摘The isotope exchange method was employed to investigate the catalytic mechanism of ionic liquid in alkylation of benzenes with olefins.It is proposed that alkylation was induced by the Lewis acid AlCl3 which attracted π electrons of 1-dodecene to shift toward 1-carbon,thus forming a carbonium ion.The carbonium ion further reacted with benzenes to form a complex.Due to unstabilit of the complex,a deuterated ring proton was transferred into an electronegative 1-carbon of the side chain to substitute for the AlCl3,accordingly 2-phenyldodecane was generated.QI GuoPeng 1,JIANG Feng 1,SUN XueWen 2 & ZHAO SuoQi 2 1School of Chemical Engineering,Tianjin University,Tianjin 300072,China 2State key Laboratory of Heavy Oil Processing,Petroleum University of China,Beijing 102249,China 2010Science China Chemistry2010,53,5:7
8A Trot and Flying Trot Control Method for Quadruped Robot Based on Optimal Foot Force Distribution显示文摘In order to enhance the dynamic motion capability of the bionic quadruped robot,a flying trot gait control method based on full-scale virtual model and optimal plantar force distribution is proposed.A stable flying trot gait is accomplished by mapping the robot torso motion to the foot trajectory.The force distribution calculated by the torso virtual model is converted into a quadratic optimization problem and solved in real time by the open source library Gurobi.The transition between the trot gait and the flying trot gait is achieved by coordinating leg motion phases.The results of the dynamic simulation verify that the proposed method can realize the 3D stable flying trot gait.Compared against the trot gait,the flying trot gait can improve the speed of the quadruped robot.Combine the trot gait and the flying trot gait,the quadruped robot can move efficiently and adapt to complex terrains.Teng Chen Xiaobo Sun Ze Xu Yibin Li Xuewen Rong Lelai Zhou 2019Journal of Bionic Engineering2019,16,4:4
9Insight into selective removal of copper from high-concentration nickel solutions with XPS and DFT:New technique to prepare 5N-nickel with chelating resin显示文摘An efficient and profitable separation process was proposed to prepare 5N(the purity of the metal solution reaches 99.999%) high-purity nickel from 3N nickel-solutions using Purolite S984. The adsorption performance of this superior resin, especially its selectivity for metal ions,was explored quantitatively. The maximum adsorption capacity for copper was 2.286 mmol/g calculated by the Langmuir model, which was twice as large as that for nickel. In the binary systems, the adsorption capacity for nickel was decreased by 45%, indicating direct competition for the active sites. The infinite separation factor for copper versus nickel exceeded 300, revealing the feasibility of preparing5N-level high-purity nickel solutions, which was further verified using the 800 BV(bed volume) effluent in the column dynamic process.According to the cost–benefit analysis, purification contributed to a profit of approximately 60,000 USD per cycle, and the investment return period was less than 1/3 years. Density functional theory analysis confirmed that four nitrogen atoms would be involved in the coordination complex and thus a structure involving two five-membered rings could be achieved. The X-ray photoelectron spectra confirmed the involvement of nitrogen atoms,implying a coordination ratio of approximately 1:1.Xuewen Tao Fuqiang Liu Zhiping Bai Dongyang Wei Xiaopeng Zhang Junfei Wang Jie Gao Xiaowen Sun Baohua Li Chenghui Li Aimin Li 2016Journal of Environmental Sciences2016,28,10:3
10Biochar can Increase Chinese Cabbage(Brassica oleracea L.)Yield,Decrease Nitrogen and Phosphorus Leaching Losses in Intensive Vegetable Soil显示文摘There are few evidences on the effect of biochar on vegetable yield,nitrogen(N)and phosphorus(P)leaching losses under intensive vegetable production soil.The current field plot scale study evaluated responses of Chinese cabbage(Brassica oleracea L.)yield,N and P leaching losses using five N treatments of common N application rate according to local farmers’practice(N100%),reducing 20%or 40%N fertilizer(N80%and N60%),and reducing 40%N fertilizer but incorporating 10 or 20 t/ha biochar(N60%+BC10 and N60%+BC20).Results showed that N80%and N60%decreased both the cabbage economic and leaf yields by 6.8%-36.3%and 27.4%-37.7%,respectively.Incorporation of biochar with reduced N fertilizer rates improved the cabbage yield,in particular the N60%+BC20 matched the yield that observed in N100%treatment.Enhanced N and P uptake capacities of cabbage shoot probably contributed the higher vegetable production under both biochar amendment schemes.Biochar application mitigated the NH_(4)^(+)-N and total P leaching losses by 20%-30%and 29%-32%,respectively,compared with their counterpart treatment N60%.Nevertheless,biochar exerted no influence on the NO_(3)^(-)-N leaching.In addition,soil organic matter content was recorded with 7.4%-28.7%higher following 10-20 t/ha biochar application.In conclusion,biochar application can increase economic yield of cabbage via increasing N and P use efficiency,decrease N and P leaching losses,and improve soil quality in an intensive vegetable production system.Haijun Sun Paramsothy Jeyakumar Hongdong Xiao Xuewen Li Jiayou Liu Min Yu Prabal Bir Jung Rana Weiming Shi 2022Phyton-International Journal of Experimental Botany2022,91,1:3
11The precise multimode GNSS positioning for UAV and its application in large scale photogrammetry显示文摘In this paper,we use multimode GNSS instead of single GPS constellation to resolve the three exterior line elements of each image.The principles of differential GNSS positioning and GNSSsupported aerial triangulation(AT)are presented and an implementation case is demonstrated.With multi-constellation system,the number of visible satellites is significantly increased and the geometry distribution of the satellites is well improved.The positioning accuracy and robustness are therefore getting better compared to GPS positioning.Experimental results show that differential GNSS has remarkable increment on the integer rate of ambiguity solution when GPS has few number and low elevation angle of satellites.The combined AT adjustment of GNSS resolution and 10 ground control points(GCPs)achieve the horizontal accuracy of±18 cm and vertical accuracy of±23 cm for the check points,which are comparable with traditional bundle adjustment with dense GCPs and better than GPS-supported AT.The achieved accuracy also satisfies the requirement for 1:500 topographic maps with the bonus of 84%GCPs eliminated.In conclusion,GNSS-supported AT is of feasibility and superiority for large scale Unmanned Aerial Vehicle-based photogrammetry.Hongxing Sun Leilei Li Xuewen Ding Bingxuan Guo 2016Geo-Spatial Information Science2016,19,3:3
12Revealing the catalytic mechanism of an ionic liquid with an isotope exchange method显示文摘The alkylation mechanism catalyzed by an ionic liquid (as a Lewis acid) may be different from the traditional alkylation mechanism catalyzed by Br nsted acid,especially as their initiation steps are still not clear.In this paper,an isotope exchange method is used to investigate the catalytic mechanism of AlCl 3 /butyl-methyl-imidazolium chloride ionic liquid in the alkylation of benzene with 1-dodecene.The proposed catalytic mechanism was confirmed by analysis of ionic liquid before and after reaction and of the alkylation products of deuterated benzene (C 6 D 6) with 1-dodecene.The proposed mechanism consists of the equilibrium reaction between [Al 2 Cl 7 ] +H + and [AlHCl 3 ] + +[AlCl 4 ],in which the Br nsted acid [AlHCl 3 ] + is supplied by the reaction of 2-H on the imidazolium ring and [Al 2 Cl 7 ].The alkylation reaction is initiated by the Br nsted acid [AlHCl 3 ] + which reacts with 1-dodecene to form a carbonium ion,then the carbonium ion reacts with benzene to form an unstable σ complex,leading to the formation of 2-phenyldodecane.Sun Xuewen Zhao Suoqi 2011Petroleum Science2011,8,4:3
13Investigation of free volume and the interfacial, and toughening behavior for epoxy resin/rubber composites by positron annihilation显示文摘Zeng Minfeng Sun Xudong Xiao Huiquan Ji Genzhong Jiang Xuewen Wang Baoyi Qi Chenze 2007Radiation Physics and Chemistry2007,,3:1
14Direct laser patterning of two-dimensional lateral transition metal disulfide-oxide-disulfide heterostructures for ultrasensitive sensors显示文摘Two-dimensional(2D)heterostructures based on the combination of transition metal dichalcogenides(TMDs)and transition metal oxides(TMOs)have aroused growing attention due to their integrated merits of both components and multiple functionalities.However,nondestructive approaches of constructing TMD-TMO heterostructures are still very limited.Here,we develop a novel type of lateral TMD-TMO heterostructure(NbS2-Nb2O5-NbS2)using a simple lithography-free,direct laser-patterning technique.The perfect contact of an ultrathin TMO channel(Nb2O5)with two metallic TMDs(NbS2)electrodes guarantee strong electrical signals in a two-terminal sensor.Distinct from sensing mechanisms in separate TMOs or TMDs,this sensor works based on the modulation of surface conduction of the ultrathin TMO(Nb2O5)channel through an adsorbed layer of water molecules.The sensor thus exhibits high selectivity and ultrahigh sensitivity for room-temperature detection of NH3(ΔR/R=80%at 50 ppm),superior to the reported NH3 sensors based on 2D materials,and a positive temperature coefficient of resistance as high as 15%–20%/℃.Bending-invariant performance and high reliability are also demonstrated in flexible versions of sensors.Our work provides a new strategy of lithography-free processing of novel TMD-TMO heterostructures towards high-performance sensors,showing great potential in the applications of future portable and wearable electronics.Bolun Wang Hao Luo Xuewen Wang Enze Wang Yufei Sun Yu-Chien Tsai Jinxuan Dong Peng Liu Huanglong Li Yong Xu Sefaattin Tongay Kaili Jiang Shoushan Fan Kai Liu 2020Nano Research2020,13,8:1
15New alkylation route of benzene with ethylene catalyzed by FeC13 - C1 ionic liquid 显示文摘Sun Xuewen Zhao Suoqi Wang Renan 2004China J Chem Eng2004,12,5:1
16Simulation Test on Charge Density and Surface Potential in an Oil Tank Dur- ing Filling Operation显示文摘Keping Sun Quanzhen Liu Xuewen Li 2009Journal of Electrostatics2009,67,:1
17Field experiment using transient energy method to locate a single-phase to ground fault显示文摘Distribution networks in China and several other countries are predominantly neutral inefficiently grounding systems(NIGSs),and more than 80%of the faults in distribution networks are single-phase-to-ground(SPG)faults.Because of the weak fault current and imperfect monitoring equipment configurations,methods used to determine the faulty line secti ons with SPG faults in NIGSs are in effective.The developme nt and application of distributi on-level phasor measurement units(PMUs)provide further comprehensive fault information for fault diagnosis in a distribution network.When an SPG fault occurs,the transient energy of the faulted line section tends to be higher than the sum of the transient energies of other line sections.In this regard,transient energy-based fault location algorithms appear to be a promising resolution.In this study,a field test plan was designed and implemented for a 10 kV distribution network.The test results dem on strate the effective ness of the transient en ergy-based SPG locati on method in practical distributi on networks.Wei Xie Xuewen Wang Chen Fang Hengxu Zhang Fang Shi Xiaodong Xing Baicong Sun 2020Global Energy Interconnection2020,3,6:1
18Structure and Morphology of Ti-A1 Composite Powders Treated by Mechanical Alloying 显示文摘Li Xuewen Sun Hongfei Fang Webin 2011Transactions of Nonferrous Metals Society of China2011,21,2:1
19Simulation and experimental study on the surface morphology and energy lost of the target material under non-overlapping impact of angular particles显示文摘In order to further understand the effect of solid impurities on pipeline wall during erosion,the particle impact process without fluid was extracted for specific study.The effect of multi-impact particles on the wall of pipeline was studied experimentally and simulated.In this experiment,an improved ejection apparatus was implemented to carry out multi-impacts non-overlapping impingement by rhombic particles made of high speed steel(W18Cr4V)on the AA6061 aluminum alloy plate through changing particle angle,incident angle,orientation angle and impact velocity.As a result,each particle's penetration depth was investigated and particles' rebound trajectory can be described through this experiment as well as surface morphology of the target material after impingement.The ductile damage criterion,shear damage criterion and MSFLD damage criterion were jointly implemented in ABAQUS/CAE software to simulate the whole process of collision which proved to be effective by getting consistent result compared with experimental data.It is found that under the condition of continuous non-overlapping impact,the target material produces a small work hardening effect in the impact area by converting kinetic energy of moving particles into internal energy of plate so as to reduce the penetration depth of each impact particle.Xuewen Cao Chenyang Fu Zhenqiang Xie Chao Wu Xiaoyang Sun 2021Chinese Journal of Chemical Engineering2021,34,1:1
20Peptaibols:Diversity, bioactivity, and biosynthesis显示文摘Peptaibols are a large family of linear,amphipathic polypeptides consisting of 5-20 amino acid residues gener-ated from the fungal nonribosomal peptide synthetase(NRPS)pathway.With a relatively high content of non-proteinogenic amino acids such as��-aminoisobutyrate(Aib)and isovaline(Iva)in the skeleton,peptaibols exhibit a wide range of biological activities,including anti-microbial,cytotoxic,and neuroleptic effects.With five peptai-bols brought to market for use as biocontrol agents,this class of peptides has received increasing attention from both biochemists and pharmacologists.In this review,we summarized the progress made in structural character-ization,elucidation of biosynthetic pathways,and investigation of biosynthesis elucidation and bioactivities,to promote further efforts to develop peptaibols as pharmaceuticals.Xuewen Hou Ruonan Sun Yanyan Feng Runfang Zhang Tianjiao Zhu Qian Che Guojian Zhang Dehai Li 2022Engineering Microbiology2022,2,3:1
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