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| 1 | Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1显示文摘Ferroptosis is a form of regulated cell death, characterized by excessive membrane lipid peroxidation in an iron-and ROS-dependent manner. Celastrol, a natural bioactive triterpenoid extracted from Tripterygium wilfordii, shows effective anti-fibrotic and anti-inflammatory activities in multiple hepatic diseases. However, the exact molecular mechanisms of action and the direct protein targets of celastrol in the treatment of liver fibrosis remain largely elusive. Here, we discover that celastrol exerts anti-fibrotic effects via promoting the production of reactive oxygen species(ROS) and inducing ferroptosis in activated hepatic stellate cells(HSCs). By using activity-based protein profiling(ABPP) in combination with bio-orthogonal click chemistry reaction and cellular thermal shift assay(CETSA), we show that celastrol directly binds to peroxiredoxins(PRDXs), including PRDX1, PRDX2, PRDX4 and PRDX6,through the active cysteine sites, and inhibits their anti-oxidant activities. Celastrol also targets to heme oxygenase 1(HO-1) and upregulates its expression in activated-HSCs. Knockdown of PRDX1, PRDX2,PRDX4, PRDX6 or HO-1 in HSCs, to varying extent, elevated cellular ROS levels and induced ferroptosis. Taken together, our findings reveal the direct protein targets and molecular mechanisms via which celastrol ameliorates hepatic fibrosis, thus supporting the further development of celastrol as a promising therapeutic agent for liver fibrosis. | Piao Luo Dandan Liu Qian Zhang Fan Yang Yin-Kwan Wong Fei Xia Junzhe Zhang Jiayun Chen Ya Tian Chuanbin Yang Lingyun Dai Han-Ming Shen Jigang Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 14 |
| 2 | The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection显示文摘A fluorescence polarization immunoassay(FPIA)was developed for the analysis ofaflatoxins(AFs)using an anti-aflatoxin B1(AFB1)monoclonal antibody and a novel fluorescein-labeled AFB1tracer.The FPIA showed an IC50 value of 23.33ng/mL with a limit of detection of 13.12 ng/mL for AFB1.The cross-reactivities of AFB1,AFB2,AFG1,AFG2,AFM1,and AFM2 with the antibody were100%,65.7%,143%,23.5%,111.4%,and 2%,respectively.The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs,albeit not AFG2 and AFM2.The total time required for analyzing 96 samples in one microplate was less than 5 min.This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs. | SHENG Ya Jie EREMIN Sergei MI Tie Jun ZHANG Su Xia SHEN Jian Zhong WANG Zhan Hui | 2014 | Biomedical and Environmental Sciences2014，27，2： | 10 |
| 3 | BRAF^(V600E) vs. TIRADS in predicting papillary thyroid cancers in Bethesda system Ⅰ, Ⅲ, and Ⅴ nodules显示文摘Objective: Bethesda System for Reporting Thyroid Cytopathology(BSRTC) categories Ⅰ, Ⅲ, and Ⅴaccount for a significant proportion of fine needle aspiration cytology(FNAC) diagnoses. This study aimed to compare the diagnostic efficacy of BRAF^(V600E) mutation and the Thyroid Imaging Reporting and Data System(TIRADS) classification in differentiating papillary thyroid cancers(PTCs) from benign lesions among BSRTC I, III, and V nodules.Methods: A total of 472 patients with 479 nodules were enrolled in this prospective study. Ultrasound, BRAF^(V600E) mutation testing, and FNAC were performed in each nodule, followed by surgery or regular ultrasound examination.Results: In the BSRTC I category, BRAF^(V600E) showed similar sensitivity, higher specificity, and lower accuracy when compared with TIRADS. In the BSRTC III/V category, the sensitivity, specificity, and accuracy of BRAF^(V600E) were similar to those of TIRADS. In comparison to BRAF^(V600E) alone, the combination of the two methods significantly improved sensitivity(BSRTC Ⅰ:93.6% vs. 67.7%, P < 0.01; BSRTC Ⅲ: 93.8% vs. 75.0%, P < 0.01; BSRTC V: 96.0% vs. 85.3%, P < 0.001). When compared with TIRADS alone, the combination improved sensitivity in BSRTC Ⅰ nodules(93.6% vs. 74.2%, P < 0.05), increased sensitivity and decreased accuracy in BSRTC III nodules(93.8% vs. 75.0%, P < 0.01, 91.0% vs. 93.6%, P < 0.01), and improved both sensitivity and accuracy in BSRTC V nodules(96.0% vs. 82.0%, P < 0.001; 94.2% vs. 81.3%, P < 0.001).Conclusions: BRAF^(V600E) exhibited higher specificity and lower accuracy compared with TIRADS in BSRTC Ⅰ nodules, while the two methods showed similar diagnostic value in BSRTC Ⅲ/Ⅴ nodules. The combination of the two methods distinctly improved sensitivity in the diagnosis of PTCs in BSRTC Ⅰ, Ⅲ, and Ⅴ nodules. | Ya Wu Ting Xu Xingyue Cao Xin Zhao Hongyan Deng Jianxiang Wang Xiao Li Qing Yao Xinhua Ye Meiping Shen Xiaohong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 9 |
| 4 | New Process for Extracting Oil and Starch from Tea Seeds显示文摘 | Jinzhong Jiang Cuijuan Ren Zibu Wang Changjiang Qian Yuxiao Shen Ya Yang | 2013 | Journal of Food Science and Engineering2013，3，12： | 8 |
| 5 | NGX6 inhibits AP-1 and Ets-1 expression and down-regulates cyclin D1 in human coloreetal cancer显示文摘Colorectal 癌症(CRC ) 是与病态和死亡的增加的发生被联系的一个普通恶意的肿瘤。鼻咽的联系癌的基因(NGX6 ) 6 是肿瘤转移的新奇候选人 suppressor 基因,它在 CRC 是下面调整的。在现在的学习,我们构造了 colorectal 织物 microarray 在 CRC 纸巾检验 NGX6, phospho-c-Jun N 终端 kinase (p-JNK ) ,和 phospho 细胞外的调整信号的 kinase (津贴) 的表示侧面。而 p-JNK 和津贴的表情在 CRC 纸巾是更高的,我们发现 NGX6 表示在 CRC 纸巾和变形淋巴节点是更低的,比在正常肠的 mucosa。NGX6, p-JNK,和津贴的表情与 colorectal 纸巾的临床的病理学的特征被联系。NGX6 overexpression 禁止了激活和 JNK1 的原子 translocation,它在细胞质导致了 p-JNK 的累积,但是没禁止激活和 ERK1/2 的原子 translocation。NGX6 也禁止了抄写因素 AP-1 (c-jun 和 c-fos ) 和 Ets-1 的表示。另外, NGX6 overexpression 减少了 cyclin D1 并且戏剧性地的表示压制了 cyclin D1 倡导者的 transcriptional 效率。我们建议那 NGX6 表情沉醉于人的 colorectal carcinogenesis 的多步过程。它的 overexpression 能禁止抄写因素的表示 AP-1 和 Ets-1,和下面调整在人的 CRC 的 cyclin D1 倡导者的 transcriptional 活动。 | Ya Peng Hongtao Li Minghua Wu Xiaoyan Wang Songqing Fan Fen Liu Bo Xiang Qin Guo Xueyuan Tang Shourong Shen | 2009 | Acta Biochimica et Biophysica Sinica2009，41，6： | 8 |
| 6 | Cultured Mycelium Cordyceps sinensis alleviates CCI4-induced liver inflammation and fibrosis in mice by activating hepatic natural killer cells显示文摘目的: 最近的证据证明有教养的菌丝体 Cordyceps sinensis (CMCS ) 有效地在老鼠免于肝纤维变性。这里,我们调查了 CMCS 的 anti-fibrotic 行动是否与它肝的生来的杀手( NK )的活动的规定有关是在 CCl 4-treated mice.Methods 的房间: C57BL/6 老鼠与10% CCl 4被注射( 2 mL/kg , ip ) 3 次每为 4 个星期的星期,并且收到了 CMCS ( 120 mg· ; kg −1·d−1, ig )在这个时期期间。在实验的另一部分,老鼠也与 NK 删除房间的抗体 ASGM-1 被注射(20 μ g, ip ) 在开始的 3 个星期内的 5 次。在老鼠被牺牲以后,浆液肝功能,和肝发炎, hydroxyproline 内容和骨胶原免职被估计。肝的 NK 房间和孤立的肝淋巴细胞上的 NKG2D (NK 房间的激活受体) 的表示的数字用流动 cytometry 被分析。在肝纸巾的 Desmin 表示和房间 apoptosis 分别地用染色的 desmin 和 TUNEL 试金被学习。α 的层次; -SMA, TGF-β, RAE-1δ并且 RAE-1ε在肝,纸巾被 RT-qPCR.Results 决定: 在 CCl 4-treated 鼠标, CMCS 管理显著地改进了肝功能,稀释的肝发炎和纤维变性,并且在肝的 NK 房间上增加了肝的 NK 房间的数字和 NKG2D 的表达式水平。而且, CMCS 管理显著地减少了在肝纸巾的 desmin 表示,和邻近肝的星形的房间的染色的增加的 TUNEL。有 NK 删除房间的 ASGM-1 的注射不仅减少在 CCl 4-treated 老鼠的肝的 NK 房间的数字,而且极大地加速的肝发炎和纤维变性。在有 NK 房间弄空的 CCl 4-treated 老鼠, CMCS 管理速度肝纤维变性开发的率,并且温和地 upregulated 肝的 NK 房间,但是没有改变 NKG2D expression.Conclusion 的数字: CMCS allevi¬经由支持肝的 NK 房间的激活的 ates CCl 4-induced 肝发炎和纤维变性。CMCS 部分颠倒肝的 NK 房间的 ASGM-1-induced 弄空。 | Yuan PENG Kai HUANG Li SHEN Ya n-ya n TAO Cheng-hai LIU | 2016 | Acta Pharmacologica Sinica2016，37，2： | 8 |
| 7 | Warm temperature compromises JA-regulated basal resistance to enhance Magnaporthe oryzae infection in rice显示文摘Changes in global temperatures profoundly affect the occurrence of plant diseases.It is well known that rice blast can easily become epidemic in relatively warm weather.However,the molecular mechanism remains unclear.In this study,we show that enhanced blast development at a warm temperature(22C)compared with the normal growth temperature(28C)is rice plant-determined.Comparative transcriptome analysis revealed that jasmonic acid(JA)biosynthesis and signaling genes in rice could be effectively induced by Magnaporthe oryzae at 28C but not at 22C.Phenotypic analyses of the osaoc1 and osmyc2 mutants,OsCOI1 RNAi lines,and OsMYC2-OE plants further demonstrated that compromised M.oryzaeinduced JA biosynthesis and signaling lead to enhanced blast susceptibility at the warm temperature.Consistent with these results,we found that exogenous application of methyl jasmonate served as an effective strategy for improving blast resistance under the warm environmental conditions.Furthermore,decreased activation of JA signaling resulted in the downregulated expression of some key basal resistance genes at 22C when compared with 28C.Among these affected genes,OsCEBiP(chitin elicitorbinding protein precursor)was found to be directly regulated by OsMYB22 and its interacting protein OsMYC2,a key component of JA signaling,and this contributed to temperature-modulated blast resistance.Taken together,these results suggest that warm temperature compromises basal resistance in rice and enhances M.oryzae infection by reducing JA biosynthesis and signaling,providing potential new strategies for managing rice blast disease under warm climate conditions. | Jiehua Qiu Junhui Xie Ya Chen Zhenan Shen Huanbin Shi Naweed I.Naqvi Qian Qian Yan Liang Yanjun Kou | 2022 | Molecular Plant2022，15，4： | 5 |
| 8 | A network security entity recognition method based on feature template and CNN-BiLSTM-CRF显示文摘By network security threat intelligence analysis based on a security knowledge graph(SKG), multi-source threat intelligence data can be analyzed in a fine-grained manner. This has received extensive attention. It is difficult for traditional named entity recognition methods to identify mixed security entities in Chinese and English in the field of network security, and there are difficulties in accurately identifying network security entities because of insufficient features extracted. In this paper, we propose a novel FT-CNN-BiLSTM-CRF security entity recognition method based on a neural network CNN-BiLSTM-CRF model combined with a feature template(FT). The feature template is used to extract local context features, and a neural network model is used to automatically extract character features and text global features. Experimental results showed that our method can achieve an F-score of 86% on a large-scale network security dataset and outperforms other methods. | Ya QIN Guo-wei SHEN Wen-bo ZHAO Yan-ping CHEN Miao YU Xin JIN | 2019 | Frontiers of Information Technology & Electronic Engineering2019，20，6： | 5 |
| 9 | Thioredoxin-Interacting Protein(TXNIP) Regulates Parkin/PINK1-mediated Mitophagy in Dopaminergic Neurons Under High-glucose Conditions: Implications for Molecular Links Between Parkinson’s Disease and Diabetes显示文摘Patients with diabetes mellitus have a higher risk of developing Parkinson’s disease(PD). However, the molecular links between PD and diabetes remain unclear.In this study, we investigated the roles of thioredoxininteracting protein(TXNIP) in Parkin/PINK1-mediated mitophagy in dopaminergic(DA) cells under high-glucose(HG) conditions. In streptozotocin-induced diabetic mice,TXNIP was upregulated and autophagy was inhibited in the midbrain, while the loss of DA neurons was accelerated by hyperglycemia. In cultured PC12 cells under HG, TXNIP expression was upregulated and the intracellular reactive oxygen species(ROS) levels increased, leading to cell death. Autophagic flux was further blocked and PINK1 expression was decreased under HG conditions. Parkin expression in the mitochondrial fraction and carbonyl cyanide 3-chlorophenylhydrazone(CCCP)-induced co-localization of COX IV(marker for mitochondria) and LAMP1(marker for lysosomes) were also significantly decreased by HG. Overexpression of TXNIP was sufficient to decrease the expression of both PINK1 and Parkin in PC12 cells, while knockdown of the expression of TXNIP by si RNA decreased intracellular ROS and attenuated cellular injury under HG. Moreover, inhibition of TXNIP improved the CCCP-induced co-localization of COX IV and LAMP1 in PC12 cells under HG. Together, these results suggest that TXNIP regulates Parkin/PINK1-mediated mitophagy under HG conditions, and targeting TXNIP may be a promising therapeutic strategy for reducing the risk of PD under hyperglycemic conditions. | Cun-Jin Su Zhu Shen Ru-Xiao Cui Ya Huang De-Lai Xu Feng-Lun Zhao Jie Pan Ai-Ming Shi Tong Liu Yun-Li Yu | 2020 | Neuroscience Bulletin2020，36，4： | 5 |
| 10 | Modeling and Experimental Validation of the Electron Beam Selective Melting Process显示文摘 | Wentao Yan Ya Qian Weixin Ma Bin Zhou Yongxing Shen Feng Lin | 2017 | Engineering2017，3，5： | 5 |
| 11 | Antibiofilm peptides against biofilms on titanium and hydroxyapatite surfaces显示文摘Biofilms are the main challenges in the treatment of common oral diseases such as caries,gingival and endodontic infection and periimplantitis.Oral plaque is the origin of microbes colonizing in the form of biofilms on hydroxyapatite(tooth)and titanium(dental implant)surfaces.In this study,hydroxyapatite(HA)and titanium(Ti)disks were introduced,and their surface morphology was both qualitatively and quantitatively analyzed by a scanning electron microscope(SEM)and atomic force microscope(AFM).The average roughness of Ti disks(77.6±18.3 nm)was less than that of HA(146.1±38.5 nm)(p<0.05).Oral multispecies biofilms which were cultured on Ti and HA disks for 6 h and three weeks were visualized by SEM.We investigated the ability of two new antibiofilm peptides,DJK-5 and 1018,to induce killing of bacteria in oral multispecies biofilms on Ti and HA disks.A 6-h treatment by DJK-5 and 1018(2 or 10μg/mL)significantly reduced biomass of the multispecies biofilms on both Ti and HA disks.DJK-5 was able to kill more bacteria(40.4–75.9%)than 1018(30.4–67.0%)on both surfaces(p<0.05).DJK-5 also led to a more effective killing of microbes after a 3-min treatment of 3-day-old and 3-week-old biofilms on Ti and HA surfaces,compared to peptide 1018 and chlorhexidine(p<0.05).No significant difference was found in the amount of biofilm killing between Ti and HA surfaces.Both peptide DJK-5 and 1018 may potentially be used as effective antibiofilm agents in clinical dentistry. | Dan Wang Markus Haapasalo Yuan Gao Jingzhi Ma Ya Shen | 2018 | Bioactive Materials2018，3，4： | 5 |
| 12 | High-Q microresonators on 4H-silicon-carbide-on-insulator platform for nonlinear photonics显示文摘The realization of high-quality(Q)resonators regardless of the underpinning material platforms has been a ceaseless pursuit,because the high-Q resonators provide an extreme environment for confining light to enable observations of many nonlinear optical phenomenon with high efficiencies.Here,photonic microresonators with a mean Q factor of 6.75×10^(6)were demonstrated on a 4H-silicon-carbide-on-insulator(4H-SiCOI)platform,as determined by a statistical analysis of tens of resonances.Using these devices,broadband frequency conversions,including second-,third-,and fourth-harmonic generations have been observed.Cascaded Raman lasing has also been demonstrated in our SiC microresonator for the first time,to the best of our knowledge.Meanwhile,by engineering the dispersion properties of the SiC microresonator,we have achieved broadband Kerr frequency combs covering from 1300 to 1700nm.Our demonstration represents a significant milestone in the development of SiC photonic integrated devices. | Chengli Wang Zhiwei Fang Ailun Yi Bingcheng Yang Zhe Wang Liping Zhou Chen Shen Yifan Zhu Yuan Zhou Rui Bao Zhongxu Li Yang Chen Kai Huang Jiaxiang Zhang Ya Cheng Xin Ou | 2021 | Light(Science & Applications)2021，10，8： | 4 |
| 13 | Stable Lithium-Carbon Composite Enabled by Dual-Salt Additives显示文摘Lithium metal is regarded as the ultimate negative electrode material for secondary batteries due to its high energy density.However,it suffers from poor cycling stability because of its high reactivity with liquid electrolytes.Therefore,continuous efforts have been put into improving the cycling Coulombic efficiency(CE)to extend the lifespan of the lithium metal negative electrode.Herein,we report that using dual-salt additives of LiPF_(6) and LiNO_(3) in an ether solvent-based electrolyte can significantly improve the cycling stability and rate capability of a Li-carbon(Li-CNT)composite.As a result,an average cycling CE as high as 99.30% was obtained for the Li-CNT at a current density of 2.5 mA cm^(-2) and an negative electrode to positive electrode capacity(N/P)ratio of 2.The cycling stability and rate capability enhancement of the Li-CNT negative electrode could be attributed to the formation of a better solid electrolyte interphase layer that contains both inorganic components and organic polyether.The former component mainly originates from the decomposition of the LiNO_(3) additive,while the latter comes from the LiPF_(6)-induced ring-opening polymerization of the ether solvent.This novel surface chemistry significantly improves the CE of Li negative electrode,revealing its importance for the practical application of lithium metal batteries. | Lei Zheng Feng Guo Tuo Kang Yingzhu Fan Wei Gu Yayun Mao Ya Liu Rong Huang Zhiyun Li Yanbin Shen Wei Lu Liwei Chen | 2021 | Nano-Micro Letters2021，13，7： | 3 |
| 14 | Inhibiting Hv1 channel in peripheral sensory neurons attenuates chronic inflammatory pain and opioid side effects显示文摘Both opioids and nonsteroidal anti-inflammatory drugs(NSAIDS)produce deleterious side effects and fail to provide sustained relief in patients with chronic inflammatory pain.Peripheral neuroinflammation(PN)is critical for initiation and development of inflammatory pain.A better understanding of molecular mechanisms underlying PN would facilitate the discovery of new analgesic targets and the development of new therapeutics.Emerging evidence suggests that peripheral sensory neurons are not only responders to painful stimuli,but are also actively engaged in inflammation and immunity,whereas the intrinsic regulatory mechanism is poorly understood.Here we report the expression of proton-selective ion channel Hv1 in peripheral sensory neurons in rodents and humans,which was previously shown as selectively expressed in microglia in mammalian central nervous system.Neuronal Hv1 was up-regulated by PN or depolarizing stimulation,which in turn aggravates inflammation and nociception.Inhibiting neuronal Hv1 genetically or by a newly discovered selective inhibitor YHV98-4 reduced intracellular alkalization and ROS production in inflammatory pain,mitigated the imbalance in downstream SHP-1-pAKT signaling,and also diminished pro-inflammatory chemokine release to alleviate nociception and morphine-induced hyperalgesia and tolerance.Thus,our data reveal neuronal Hv1 as a novel target in analgesia strategy and managing opioids-related side effects. | Qiansen Zhang Yimin Ren Yiqing Mo Peipei Guo Ping Liao Yuncheng Luo Jie Mu Zhuo Chen Yang Zhang Ya Li Linghui Yang Daqing Liao Jie Fu Juwen Shen Wei Huang Xuewen Xu Yanyan Guo Lianghe Mei Yunxia Zuo Jin Liu Huaiyu Yang Ruotian Jiang | 2022 | Cell Research2022，32，5： | 3 |
| 15 | Rapid Internal Control Reference Recombinase-Aided Amplification Assays for EBV and CMV Detection显示文摘Epstein-Barr virus(EBV)and cytomegalovirus(CMV),two of the most prevalent human herpesviruses,cause a wide spectrum of diseases and symptoms and are associated with serious health problem.In this study,we developed an internal control reference recombinase-aided amplification(ICR-RAA)assay for the rapid detection of EBV and CMV within 30 min.The assay had a sensitivity of 5 and 1 copies/test for EBV and CMV,respectively,with no cross reaction with other pathogens.In comparison with those of the commercial quantitative polymerase chain reaction(qPCR),the sensitivity of the EBV and CMV ICR-RAAs using extracted DNA was 93.33%and 84.84%,respectively;the specificity was 98.75%and 100.00%,respectively;and the Kappa values were 0.930 and 0.892(P<0.05),respectively.In comparison with those of qPCR,the sensitivity of the EBV and CMV ICR-RAAs using the DNA by thermal lysis was 72.22%and 80.00%,respectively;the specificity was 100.00%。 | GAO Yuan TIE Yan Qing ZHAO Lin Qing TAN He DING Nan DING Ya Xin GUO Qi ZHANG Rui Qing WANG Jin Rong CHEN Zi Wei FAN Guo Hao SHEN Xin Xin FENG Zhi Shan MA Xue Jun | 2021 | Biomedical and Environmental Sciences2021，34，8： | 3 |
| 16 | Dental plaque-inspired versatile nanosystem for caries prevention and tooth restoration显示文摘Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,intervene and interrupt the development of caries while restoring defects.In this study,inspired by natural dental plaque,a stimuli-responsive multidrug delivery system(PMs@NaF-SAP)has been developed to prevent tooth decay and promote enamel restoration.Classic spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond,followed by conjugation with salivary-acquired peptide(SAP)to endow the nanoparticle with strong adhesion to tooth enamel.The constructed PMs@NaF-SAP specifically adheres to tooth,identifies cariogenic conditions and intelligently releases drugs at acidic pH,thereby providing antibacterial adhesion and cariogenic biofilm resistance,and restoring the microarchitecture and mechanical properties of demineralized teeth.Topical treatment with PMs@NaF-SAP effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal tissues.These findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria. | Yue Xu Yuan You Luyao Yi Xiaoyi Wu Yaning Zhao Jian Yu He Liu Ya Shen Jingmei Guo Cui Huang | 2023 | Bioactive Materials2023，，2： | 3 |
| 17 | Detailed resume of RNA m^(6)A demethylases显示文摘N6-Methyladenosine(m^(6)A) is the most abundant internal modification in eukaryotic mRNA,playing critical role in various bioprocesses. Like other epigenetic modifications, m^(6)A modification can be catalyzed by the methyltransferase complex and erased dynamically to maintain cells homeostasis. Up to now, only two m^(6)A demethylases have been reported, fat mass and obesity-associated protein(FTO)and alkylation protein AlkB homolog 5(ALKBH5), involving in a wide range of mRNA biological progress, including mRNA shearing, export, metabolism and stability. Furthermore, they participate in many significantly biological signaling pathway, and contribute to the progress and development of cancer along with other diseases. In this review, we focus on the studies about structure, inhibitors development and biological function of FTO and ALKBH5. | Dandan Shen Bo Wang Ya Gao Lijuan Zhao Yaping Bi Jinge Zhang Ning Wang Huiqin Kang Jingru Pang Ying Liu Luping Pang Zhe-Sheng Chen Yi-Chao Zheng Hong-Min Liu | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 3 |
| 18 | A novel ionic liquid based on small size cation显示文摘包含小尺寸阳离子的离子的液体材料被综合,它的作文和结构被英尺红外决定, 1 H NMR, 13 C NMR 和元素分析。然而,它象融化那样的物理性质指,粘性,电导率,等等被测量,结果证明新奇离子的液体由小阳离子组成与高度在报导离子的液体家庭之中很稀罕的对称和更大的阴离子。 | Qing Hai Cai Ya Wei Hou Shen Yi Shi Bin Lu Yong Kui Shan | 2009 | Chinese Chemical Letters2009，20，1： | 3 |
| 19 | Allotransplantation of adult spinal cord tissues after complete transected spinal cord injury: long-term survival and functional recovery in canines显示文摘Spinal cord injury(SCI), especially complete transected SCI, leads to loss of cells and extracellular matrix and functional impairments. In a previous study, we transplanted adult spinal cord tissues(aSCTs) to replace lost tissues and facilitate recovery in a rat SCI model. However, rodents display considerable differences from human patients in the scale, anatomy and functions of spinal cord systems, and responses after injury. Thus, use of a large animal SCI model is required to examine the repair efficiency of potential therapeutic approaches. In this study, we transplanted allogenic aSCTs from adult dogs to the lesion area of canines after complete transection of the thoracic spinal cord, and investigated the long-term cell survival and functional recovery. To enhance repair efficiency, a growth factor cocktail was added during aSCT transplantation, providing a favorable microenvironment. The results showed that transplantation of a SCTs, in particular with the addition of growth factors, significantly improves locomotor function restoration and increases the number of neurofilament-, microtubule-associated protein2-, 5-hydroxytryptamine-, choline acetyltransferase-and tyrosine hydroxylase-positive neurons in the lesion area at 6 months post-surgery. In addition, we demonstrated that donor neurons in a SCTs can survive for a long period after transplantation. This study showed for the first time that transplanting aSCTs combined with growth factor supplementation facilitates reconstruction of injured spinal cords, and consequently promotes long lasting motor function recovery in a large animal complete transected SCI model, and therefore could be considered as a possible therapeutic strategy in humans. | He Shen Shuyu Wu Xi Chen Bai Xu Dezun Ma Yannan Zhao Yan Zhuang Bing Chen Xianglin Hou Jiayin Li Yudong Cao Xianyong Fu Jun Tan Wen Yin Juan Li Li Meng Ya Shi Zhifeng Xiao Xingjun Jiang Jianwu Dai | 2020 | Science China(Life Sciences)2020，63，12： | 3 |
| 20 | Pterostilbene Ameliorates Glycemic Control,Dyslipidemia and Liver Injury in Type 2 Diabetes Rats显示文摘Type 2 diabetes mellitus(T2DM)is typified by the increment of chronic blood glucose levels that is caused by an absolute and/or a relative deficiency of insulin,accounts for 90%of diabetes and causes a range of complications[1]. | ZHANG Yu Jing SUN Hua Lei WANG Teng LIU Xin Xin LIU Chang SHEN Fang WANG Bing Ya DING Run Rong LIU Yi Ming HUANG Guo Yu LI Wen Jie LI Xing | 2020 | Biomedical and Environmental Sciences2020，33，5： | 3 |
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