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| 1 | Systemic immune-inflammation index for predicting prognosis of colorectal cancer显示文摘AIM To investigate the clinical significance of preoperative systemic immune-inflammation index(SII) in patients with colorectal cancer(CRC). METHODS A retrospective analysis of 1383 cases with CRC was performed following radical surgery. SII was calculated with the formula SII =(P × N)/L, where P, N, and L refer to peripheral platelet, neutrophil, and lymphocyte counts, respectively. The clinicopathological features and follow-up data were evaluated to compare SII with other systemic inflammation-based prognostic indices such as the neutrophil-lymphocyte ratio(NLR) and platelet-lymphocyte ratio(PLR) in patients with CRC.RESULTS The optimal cut-off point for SII was defined as 340. The overall survival(OS) and disease-free survival(DFS) were better in patients with low NLR, PLR, and SII(P < 0.05). The SII was an independent predictor of OS and DFS in multivariate analysis. The area under the receiver-operating characteristics(ROC) curve for SII(0.707) was larger than those for NLR(0.602) and PLR(0.566). In contrast to NLR and PLR, SII could effectively discriminate between the TNM subgroups. CONCLUSION SII is a more powerful tool for predicting survival outcome in patients with CRC. It might assist the identification of high-risk patients among patients with the same TNM stage. | Jian-Hui Chen Er-Tao Zhai Yu-Jie Yuan Kai-Ming Wu Jian-Bo Xu Jian-Jun Peng Chuang-Qi Chen Yu-Long He Shi-Rong Cai | 2017 | World Journal of Gastroenterology2017，23，34： | 79 |
| 2 | Cold-chain food contamination as the possible origin of COVID-19 resurgence in Beijing显示文摘COVID-19,caused by SARS-CoV-2[1,2],has been contained in China through stringent non-pharmaceutical interventions.Border control and quarantine have effectively prevented the virus from being spread by infected travellers,but the risk of resurgence caused by other routes of introduction and transmission remains unclear,and current strategies to prevent resurgence could be flawed.Since July,SARS-CoV-2 RNA contaminations in frozen food imported from countries with ongoing epidemics have been reported in nine provinces in China[3,4].However,there is no robust evidence of COVID-19 outbreaks initiated by environmentto-human transmission.Here we add to evidence of such transmission by investigating the recent COVID-19 resurgence in Beijing. | Xinghuo Pang Lili Ren Shuangsheng Wu Wentai Ma Jian Yang Lin Di Jie Li Yan Xiao Lu Kang Shichang Du Jing Du Jing Wang Gang Li Shuguang Zhai Lijuan Chen Wenxiong Zhou Shengjie Lai Lei Gao Yang Pan Quanyi Wang Mingkun Li Jianbin Wang Yanyi Huang Jianwei Wang COVID-19 Field Response Group COVID-19 Laboratory Testing Group | 2020 | National Science Review2020，7，12： | 51 |
| 3 | Traditional herbal medicine in preventing recurrence after resection of small hepatocellular carcinoma: a multicenter randomized controlled trial显示文摘BACKGROUND: Disease recurrence is a main challenge in treatment of hepatocellular carcinoma (HCC). There is no generally accepted method for preventing recurrence of HCC after resection. OBJECTIVE: To compare the efficacy of a traditional herbal medicine (THM) regimen and transarterial chemoembolization (TACE) in preventing recurrence in post-resection patients with small HCC. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS: This is a multicenter, open-label, randomized, controlled study, which was undertaken in five centers of China. A total of 379 patients who met the eligibility criteria and underwent randomization were enrolled in this trial. One hundred and eighty-eight patients were assigned to the THM group and received Cinobufacini injection and Jiedu Granule, and the other 191 patients were assigned to the TACE group and received one single course of TACE. MAIN OUTCOME MEASURES: Primary outcome measures were the annual recurrence rate and the time to recurrence. Incidence of adverse events was regarded as the secondary outcome measure. RESULTS: Among the 364 patients who were included in the intention-to-treat analysis, 67 patients of the THM group and 87 of the TACE group had recurrence, with a hazard ratio of 0.695 (P = 0.048). Median recurrence-free survival of the patients in the THM and TACE groups was 46.89 and 34.49 months, respectively. Recurrence rates at 1, 2 and 3 years were 17.7%, 33.0% and 43.5% for the THM group, and 28.8%, 42.5% and 54.0% for the TACE group, respectively (P = 0.026). Multivariate analysis indicated that the THM regimen had a big advantage for prolonging the recurrence-free survival. Adverse events were mild and abnormality of laboratory indices of the two groups were similar. CONCLUSION: In comparison with TACE therapy, the THM regimen was associated with diminished risk of recurrence of small-sized HCC after resection, with comparable adverse events. TRIAL REGISTRTION IDENTIFIER: This trial was registered in the Chinese Clinical Trial Registry with the identifier ChiCTR-TRC-07000033. | Xiao-feng Zhai Zhe Chen Bai Li Feng Shen Jia Fan Wei-ping Zhou Yun-ke Yang Jing Xu Xiao Qin Le-qun Li Chang-quan Ling | 2013 | Journal of Integrative Medicine2013，11，2： | 49 |
| 4 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 5 | Regional assessment of cadmium pollution in agricultural lands and the potential health risk related to intensive mining activities: A case study in Chenzhou City, China显示文摘The purpose of this study was to assess the extent of cadmium (Cd) contamination in agricultural soil and its potential risk for people. Soils, rice, and vegetables from Chenzhou City, Southern China were sampled and analyzed. In the surface soils, the 95% confidence interval for the mean concentration of Cd varied between 2.72 and 4.83 mg/kg (P < 0.05) in the survey, with a geometric mean concentration of 1.45 mg/kg. Based on the GIS map, two hot spot areas of Cd in agricultural soils with high Cd concentrations were identified to be located around the Shizhuyuan, Jinshiling, and Yaogangxian mines, and the Baoshan and Huangshaping mines, in the center of the city. About 60% of the total investigated area, where the agricultural soil Cd concentration is above 1 mg/kg, is distributed in a central belt across the region. The critical distances, at which the soil Cd concentration were increased by the mining activities, from the mines of the soils were 23 km for the Baoshan mine, 46 km for the Huangshaping mine, and 63 km for the Shizhuyuan mine, respectively. These are distances calculated from models. The Cd concentrations in rice samples ranged from 0.01 to 4.43 mg/kg and the mean dietary Cd intake from rice for an adult was 191 μg/d. Results of risk indexes showed that soil Cd concentrations possessed risks to local residents whose intake of Cd from rice and vegetables grown in soils in the vicinity of the mine was 596 μg/d. | ZHAI Limei LIAO Xiaoyong CHEN Tongbin YAN Xiulan XIE Hua WU Bin WANG Lixia | 2008 | Journal of Environmental Sciences2008，20，6： | 35 |
| 6 | Prevalence estimates for primary brain tumors in China: a multi-center cross-sectional study显示文摘背景尽管在中国的死亡的第一个领先的原因是恶意的瘤(死亡, 374.1 每 100000 人年),因为关于学习试图在 PBT.Methods 的流行上报导一个全面评价的大脑 tumors.This 的流行病的特征有一些很好记录的报告,保健系统上的主要大脑肿瘤( PBT )的完整的影响完全没被描述 multicenter 大脑肿瘤( MCSBT )上的代表性的学习在里面中国在五地区性的 c 被开始59 岁;女性: 45 岁) .Conclusions 年龄标准化了 PBT 的流行是 22.52 每 100 000 (95% CI , 13.22~31.82 )为所有人口, 17.64 每 100 000 (95% CI , 9.41~25.87 )为人,和 27.94 同等 100 000 (95% CI , 17.58~38.30 )为有 PBT.Our 流行的 304 954 个案例的一张估计的人口估计的到中国产出的 2010 人口的 women.Age 标准化提供计划护理服务并且为 surviving.In 开发编程的策略的一个保守基础未来,它将是 | JIANG Tao TANG Gen-fu LIN Yi PENG Xiao-xia ZHANG Xiao ZHAI Xiu-wei PENG Xiang YANG Jin-qing HUANG Hong-er WU Nai-feng CHEN Xiao-jun XING Hou-xun SU Tong-yong WANG Zhong-cheng | 2011 | Chinese Medical Journal2011，，17： | 40 |
| 7 | The Genome Sequence Archive Family: Toward Explosive Data Growth and Diverse Data Types显示文摘The Genome Sequence Archive(GSA)is a data repository for archiving raw sequence data,which provides data storage and sharing services for worldwide scientific communities.Considering explosive data growth with diverse data types,here we present the GSA family by expanding into a set of resources for raw data archive with different purposes,namely,GSA(https://ngdc.cncb.ac.cn/gsa/),GSA for Human(GSA-Human,https://ngdc.cncb.ac.cn/gsa-human/),and Open Archive for Miscellaneous Data(OMIX,https://ngdc.cncb.ac.cn/omix/).Compared with the 2017 version,GSA has been significantly updated in data model,online functionalities,and web interfaces.GSA-Human,as a new partner of GSA,is a data repository specialized in human genetics-related data with controlled access and security.OMIX,as a critical complement to the two resources mentioned above,is an open archive for miscellaneous data.Together,all these resources form a family of resources dedicated to archiving explosive data with diverse types,accepting data submissions from all over the world,and providing free open access to all publicly available data in support of worldwide research activities. | Tingting Chen Xu Chen Sisi Zhang Junwei Zhu Bixia Tang Anke Wang Lili Dong Zhewen Zhang Caixia Yu Yanling Sun Lianjiang Chi Huanxin Chen Shuang Zhai Yubin Sun Li Lan Xin Zhang Jingfa Xiao Yiming Bao Yanqing Wang Zhang Zhang Wenming Zhao | 2021 | Genomics, Proteomics & Bioinformatics2021，19，4： | 38 |
| 8 | Reduced expression of E-cadherin/catenin complex in hepatocellular carcinomas显示文摘AIM: To examine the immunoreactivity of E-cadherin and four subtypes of catenin family in human hepatocellular carcinomas (HCCs) and to investigate the correlation between expression of E-cadherin/ catenin complex and clinicopathologic parameters of HCC patients. METHODS: An immunohistochemical study for E-cadherin and catenins was performed on 97 formalin-fixed, paraffin-embedded specimens of HCC. RESULTS: Reduced expression of E-cadherin, α-, β-, γ-catenin and p120 was observed in 69%, 76%, 63%, 71% and 73%, respectively. Both expressions of E-cadherin and catenin components were significantly correlated with tumor grade (P = 0.000). It showed significant difference between expression of catenin members and tumor stage (P = 0.003, P = 0.017, P = 0.007 and P = 0.000, respectively). The reduced expression of E-cadherin in HCCs was significantly correlated with intrahepatic metastasis (IM) and capsular invasion (P = 0.008, P = 0.03, respectively). A close correlation was also observed between the expression of catenins and the tumor size (P = 0.002, P = 0.034, P = 0.016 and P = 0.000, respectively). In addition, the expression of each catenin was found correlated with IM (P = 0.012, P = 0.049, P =0.026 and P = 0.014, respectively). No statistically significant difference was observed between the expression level of E-cadherin/catenin complex and lymph node permission, vascular invasion and satellite nodules. Interestingly, only expression of p120 showed correlation with AFP value (P = 0.035). The expression of E-cadherin was consistent with α-, β-, γ-catenin and p120 expression (P = 0.000). Finally, the abnormal expression of E-cadherin/catenin complex was significantly associated with patients' survival (P = 0.0253, P = 0.0052, P = 0.003, P = 0.0105 and P = 0.0016, respectively). Nevertheless, no component of E-cadherin/catenin complex was the independent prognostic factor of HCC patients. CONCLUSION: Down-regulated expressions of E-cadherin, catenins and p120 occur frequently in HCCs and contribute to the progression and development of tumor. It may be more exact and valuable to detect the co-expression of E-cadherin/catenin complex than to explore one of them in predicting tumor invasion, metastasis and patient's survival. | Bo Zhai He-Xin Yan Shu-Qin Liu Lei Chen Meng-Chao Wu Hong-Yang Wang | 2008 | World Journal of Gastroenterology2008，14，37： | 33 |
| 9 | Sodium tanshinone IIA sulfonate protects rat myocardium against ischemia-reperfusion injury via activation of PI3K/Akt/FOXO3A/Bim pathway显示文摘 | Mei-qi ZHANG Yue-liang ZHENG Huan CHEN Jian-feng TU Ye SHEN Jun-ping GUO Xiang-hong YANG Shu-ren YUAN Liang-zhong CHEN Jing-jie CHAI Jian-hong LU Chang-lin ZHAI | 2013 | Acta Pharmacologica Sinica2013，34，11： | 30 |
| 10 | Prognostic value of systemic immune.inflammation index in patients with gastric cancer显示文摘Background:Inflammation-based indexes have been used to predict survival and recurrence in cancer patients.Systemic immune-inflammation index(Sll) was reported to be associated with prognosis in some malignant tumors.In the present study,we aimed to explore the association between Sll and the prognosis of patients with gastric cancer.Methods:We retrospectively analyzed data from 444 gastric cancer patients who underwent gastrectomy at the First Affiliated Hospital of Sun Yat-sen University between January 1994 and December 2005.Preoperative Sll was calculated.The Chi square test or Fisher's exact test was used to determine the relationship between preoperative Sll and clinicopathologic characteristics.Overall survival(OS) rates were estimated using the Kaplan-Meier method,and the effect of Sll on OS was analyzed using the Cox proportional hazards model.Receiver operating characteristic(ROC)curves were used to compare the predictive ability of Sll,NLR,and PLR.Results:Sll equal to or higher than 660 was significantly associated with old age,large tumor size,unfavorable Borrmann classification,advanced tumor invasion,lymph node metastasis,distant metastasis,advanced TNM stage,and high carcino-embryonic antigen level,high neutrophil-lymphocyte ratio,and high platelet-lymphocyte ratio(all P<0.05).High Sll was significantly associated with unfavorable prognosis(P<0.001) and Sll was an independent predictor for OS(P=0.015).Subgroups analysis further showed significant associations between high Sll and short OS in stage Ⅰ,Ⅱ,Ⅲ subgroups(all P<0.05).Sll was superior to NLR and PLR for predicting OS in patients with gastric cancer.Conclusion:Preoperative Sll level is an independent prognostic factor for OS in patients with gastric cancer. | Kang Wang Feiyu Diao Zhijun Ye Xinhua Zhang Ertao Zhai Hui Ren Tong Li Hui Wu Yulong He Shirong Cai Jianhui Chen | 2017 | Chinese Journal of Cancer2017，36，9： | 30 |
| 11 | Predictive factors improving survival after gastric and hepatic surgical treatment in gastric cancer patients with synchronous liver metastases显示文摘背景为有胃的癌症和同步的肝转移的病人的预后是很 poor.However ,标准治疗学的策略一直不是在为从胃的癌症遗体 controversial.Methods 的肝转移的肝的外科的治疗以后的临床的利益和预示的因素不管肝的外科的治疗经历了 gastrectomy 的 105 个病人记录的井 established.The 因为有在我们在 1995 和 2010 之间的中心的同步肝唯一的转移的胃的癌症 P 0.001 ) .However ,肝的外科的治疗不是为经历了 D1 lymphadenectomy 的病人的一个预示的因素(中部的幸存, 8 个 vs.8 月; P=0.495 ) .For 加肝的外科的治疗经历了 gastrectomy 的 35 个病人, D2 lymphadenectomy ( P 0.001 ),淋巴节点转移( P=-0.015 ),并且肝转移( H1 vs.H2 和 H3 )( P=-0.017 )的程度是独立重要预示的因素因为加肝的外科的治疗的 survival.Conclusions D2 lymphadenectomy 可以为 long-te | Liu Jing Li Jing-hui Zhai Ru-jun Wei Bo Shao Ming-zhe Chen Lin | 2012 | Chinese Medical Journal2012，，2： | 27 |
| 12 | Predictive value of the Chinese group on the study of severe hepatitis B-acute-on-chronic liver failure score in the short-term prognosis of patients with hepatitis B virus-related acute-on-chronic liver failure显示文摘Background:As a large,prospective,multicenter study-based prognostic score for hepatitis B virus-related acute-on-chronic liver failure(HBV-ACLF),the Chinese group on the study of severe hepatitis B-acute-on-chronic liver failure score(COSSH-ACLFs),has been approved by some foreign scholars;however,its predictive value needs to be verified.This study investigated the predictive value of COSSH-ACLFs for short-term prognosis in Chinese patients with HBV-ACLF.Methods:This retrospective cohort study included 751 patients with HBV-ACLF admitted to the Fifth Medical Center of Chinese PLA General Hospital between January 2011 and December 2014.Spearman method was used to assess the correlation of COSSHACLFs with classical scores.Different COX multivariate regression models were used to confirm the relationship between COSSHACLFs and short-term prognosis in patients with HBV-ACLF,and stratified analysis was used to further verify the stability of this relationship.We compared the predictive powers of COSSH-ACLFs and other classical scores using area under the receiver operating characteristic curve(AUROC)and Z-test.Results:A total of 975 patients with HBV-ACLF were screened,and 751 were analyzed(623 male and 128 female).COSSH-ACLFs was the highest in patients with end-stage ACLF,followed by those with middle-and early-stage ACLF(H=211.8,P<0.001).In the fully adjusted model,COX multivariate regression analysis revealed that COSSH-ACLFs(as a continuous variable)was independently and positively correlated with mortality risk in patients with HBV-ACLF at 28 days(hazard ratio[HR]:1.37[1.22,1.53],P<0.001)and 90 days(HR:1.43[1.29,1.58],P<0.001).The same trend could be observed in the crude model and minimally adjusted model.The AUROCs of COSSH-ACLFs for 28-day and 90-day prognoses in patients with HBV-ACLF were 0.807 and 0.792,respectively,indicating a stronger predictive accuracy than those of classic models.Conclusions:COSSH-ACLFs,with a superior predictive accuracy compared with other classical scores,can strongly predict shortterm prognosis in Chinese patients with HBV-ACLF. | Jing-Jing Tong Wei Zhao Xiu-Ying Mu Xiang Xu Hai-Bin Su Xiao-Yan Liu Jing Chen Xing-Ran Zhai Yu Wang Jin-Hua Hu | 2019 | Chinese Medical Journal2019，，13： | 27 |
| 13 | Proteomics Associated with Virulence Differentiation of Curvularia lunata in Maize in China显示文摘蛋白质的一个维的电气泳动(1-DE ) ,蛋白质的 two-dimensionalelectrophoresis (2-DE ) 并且 cDNA 顺序克隆被用来学习 Curvularia lunata 的毒力区别[Cochliobolus lunatus ] 在中国从玉米(Zea maydis ) 孤立。从蛋白质的 1-DE 胶化侧面, 110 个可再现的乐队与六被分开 C 孤立。lunata CX-3, SD-6, C-152, C107-1, DD-60 和 W-18。68 个乐队(61.82%) 是多态的,建议在 isolates 之中的巨大的生物多样性。所有孤立组由 0.605 的系数价值由不同剧毒的类型组成因为实验被聚类进三。组织 1,由有高毒力的 CX-3, SD-6 和 C-152 组成比组显示了更多的蛋白质乐队第 2a 3,与低毒力由 C107-1 和 DD-60 组成。Proteomics 途径基于 2-DEtechniques 被使用识别在 CX-3 和 DD-60 与毒力区别联系的特定的蛋白质。423 个蛋白质点的一个总数被分开。从他们, 75 个特定的蛋白质点在 2-DE 胶化被显示。在他们之中, 28 个蛋白质点在 CX-3 是唯一的, 8 在两 2-DE 胶化上在 DD-60,和 39 个蛋白质点被显示出,但是在紧张不同地表示。包括三个唯一的蛋白质点和 17 差别的 Twentyprotein 点在 CX-3 表示了蛋白质点(超过 2 褶层 DD-60 ) 进一步与 MALDI-TOF MS/MS 被识别。结果显示大多数识别蛋白质被发现与毒力区别,新陈代谢,压力反应和信号转导被联系。他们之一作为 Brn1 蛋白质被识别,它被报导了在真菌与黑素生合成和毒力区别有关。与我们的以前的调查结果结合了,我们假设那 Brn1 蛋白质和它的调整产品可能涉及 C 的毒力区别。lunata。因而,我们克隆 Brn1cDNA 碎片并且在另外的真菌与碎片联盟。结果显示 Brn1 的 633-bpsequence 在 C 克隆。lunata 是高度与比较真菌逻辑的人。为在我们的情况中的 Brn1 的准确基因角色的进一步的工作是在进行之中的…… | Shufa Xu Jie Chen Lixing Liu Xiaofei Wang Xiuli Huang Yuhong Zhai | 2007 | Journal of Integrative Plant Biology2007，49，4： | 25 |
| 14 | Safety threshold of high-speed railway pier settlement based on train-track-bridge dynamic interaction显示文摘This paper presents a method to determine the safety threshold of bridge pier settlement in high-speed railways.An analytical expression of the mapping relationship between the pier settlement and the rail deformation is derived theoretically for the double block ballastless track-bridge system.By adopting the superposition of the track random irregularity and the rail deformation caused by the pier settlement as the excitation inputs,the variations of vehicle dynamics indices with pier settlement are comparatively analyzed.Then,the safety threshold of the bridge pier settlement is obtained according to the limit of vehicle running safety and ride comfort indices of the high-speed trains.Results show that the dynamics indices of different trains have different sensitivities to the pier settlement,and the train CRH2C is the most sensitive one among all the types of Chinese high-speed trains.When passing through the bridges in common span with pier settlement at the speed of 250–350 km/h,the trains suffer the low-frequency excitations,and the vertical acceleration of car body is most sensitive to the pier settlement of all the dynamics indices.When the car body vertical acceleration just exceeds the allowable limit,the critical settlement value is 23.4 mm,which is much bigger than the pier differential settlement limit in the current code for Chinese high-speed railways. | CHEN Zhao Wei ZHAI Wan Ming CAI Cheng Biao SUN Yu | 2015 | Science China(Technological Sciences)2015，58，2： | 26 |
| 15 | Expression and clinical significance of high risk human papillomavirus and invasive gene in cervical carcinoma显示文摘Objective:To study the expression of E6 and E7 mRNA in high-risk human papillomavirus(HPV) HPV-18 and the relationship between the expression of invasive gene and cervical carcinoma.Methods:A total of 119 patients with cervical cancer,cervical erosion and cervical HPV infection who were diagnosed in our hospital were selected and randomly divided into two groups:cervical cancer group(n= 58) and non-cancerous group(n= 61).Another 60 patients with uterine leiomyoma were selected as normal control group.Detection of HPV18 E6,E7 mRNA expression and invasion,migration,proliferation inhibition genes,epithelial mesenchymal transition genes and proliferation related protein content.Results:The relative expression of E6 and E7 HPV-18 in cervical cancer group was significant higher than that in non-cancerous group and control group(mRNA)(P<0.05).The content of TRAF6 and c-FLIP in invasive cervical cancer group was significantly higher than that in non-cancerous group and control group(P<0.05).The mR NA content of CD44v6 and MMP-9 in cervical cancer group was significantly higher than that in non-cancerous group and control group(P<0.05).The content of DEC-1,IKK16,MBP-1 in cervical cancer group was significant lower than that in non-cancerous group and control group(P<0.05).The mR NA content of beta-catenin and Vimentin in cervical cancer group was significantly lower than that in non cancerous group and control group(P<0.05).The proliferation related protein E2F1 of cervical cancer group was significantly lower than that of non-cancerous group and control group,Bmi-1 content was significantly higher than non-cancerous group and control group(P<0.05).Conclusions:The expression of the detection of cervical cancer in high-risk human papilloma virus HPV-18 E6 and E7 mRNA,and the invasion,migration,proliferation inhibition gene,epithelial mesenchymal transition and proliferation related gene protein content,HPV expression rate of mR NA increased with the development of cervical cancer,the expression is also enhanced.The expression has a certain correlation between the level and development of cervical cancer.Through the above indicators,the development of cervical cancer monitoring and treatment to provide important clinical guidance. | Zhai Lu Hua Chen Xiao-Mei Zheng Man-Ling Chen | 2017 | Asian Pacific Journal of Tropical Medicine2017，10，2： | 24 |
| 16 | Value of transthoracic echocardiography combined with cardiac troponin I in risk stratification in acute pulmonary thromboembolism显示文摘背景尖锐肺的 thromboembolism (无尾猿) 引起恰好室的机能障碍(RVD ) 和心脏的 troponin 我(cTnI ) 举起。有 RVD 和 cTnl 举起的病人有更坏的预后。因此, RVD 的早察觉和 cTnl 举起为风险层化是有益的。在这研究,我们估计了 14 天的不利临床的事件并且在 APEpatients 的广谱之中在风险层化把 RVD ontransthoracic 回响心动描记法(TTE ) 与 cTnl 相结合。未来的多中心试用从 12collaborating 医院带着证实的无尾猿包括了 90 个病人的方法。TTE 上的尖锐 RVD 面对至少 2 下列被诊断:恰好室的膨胀(没有肥大) ,劣等的静脉腔(IVC ) 的吸入的倒塌的损失,恰好室(RV ) 运动机能减退,有三个尖头的丢回的喷气 velocity>2.8 m/s。学习病人在表示根据临床的 andechocardiographic 调查结果被划分成二个组:组Ⅰ:有 RVD 的 50 个病人;组Ⅱ:没有 RVD 的 40 个病人。多于病人的一半结果(50/90, 55.6%) 有的 RVD。(26/90,28.9%) 将近三分之一在表示病人提高了 cTnl 并且仅仅 4.2% 在在起始的治疗以后的第四天。一个多重逻辑回归模型暗示 RVD,正确、左的室的 end-diastolicdiameter 比率(RVED/LVED ) ,和 cTnI 独立地预言不利 14 天的临床的结果(P<0.01 ) 。接收装置操作特征(巨鸟) 曲线表明产出最高的区别力量的截止价值 ofRVED/LVED 和 cTnl 分别地是 0.65 和 0.11 ng/ml。而且,在有 RVD 和 elevatedcTnl 的病人的一个不利 14 天的临床的事件的发生更大(40.7%) 比在有提高的 cTnI 或积极 RVD 的病人独自一个(0% 和 8.3% ,分别地)(P<0.001 ) 。结论 RVD, RVED/LVED,和 cTnI 是 14-dayclinical 结果的独立预言者。有比 0.65 大的 RVED/LVED 和比在表示的 0.11 ng/ml 高的 cTnI 的病人可能有不利 14 天的事件。与 cTnI 相结合的 RVD 能与更多看守预后识别无尾猿病人的亚群。 | ZHU Ling YANG Yuan-hua WU Ya-feng ZHAI Zhen-guo WANG Chen | 2007 | Chinese Medical Journal2007，，1： | 22 |
| 17 | Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment. | Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show | 2020 | Cell Research2020，30，10： | 19 |
| 18 | Effects of hypoxia,hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 in hepatic stellate cells显示文摘AIM To study the effects of hypoxia, hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 (MMP-2) in hepatic stellate cells ( HSC).``METHODS The expressions of MMP-2, tissue inhibitor of mjatrix metalloproteinese-2 ( TIMP-2 ) and membrane type matrix metalloproteiness-l (MT1-MMP) in cultured rat HSC were detected by immunocytochemistry (ICC) and in situ hybridization (ISH). The contents of MMP-2 and TIMP-2 in culture supernatant were detected with ELISA and the activity of MMP-2 in supematant was revealed by zymography.``RESULTS In the situation of hypoxia for 12 h, the expression of MMP-2 protein wss enhenced (hypoxiagroup positive indexes: 5.7 ± 2.0, n = 10; control: 3.2 ±1 .0. n -- 7; P<0.05). while TIMP-2 protein wss decreased in HSC ( hypoxia group positive indexes: 2.5 ± 0.7, n =10: control: 3.6 ± 1.0, n = 7; P | Ping-Sheng Chen~(1,2) Wei-Rong Zhai~1 Xiao-Mei Zhou~3 Jin-Sheng Zhang~1 Yue-E Zhang~1 Yu-Qin Ling~1 Ying-Hong Gu~1 1 Department of Pathology,School of Basic Medical Sciences,Fudan University,Shanghai 200032,China2 Ping-Sheng Chen now works in the Department of Pathology,School of Basic Medical Sciences the (former Nanjing Railway Medical College),Southeast University,Nanjing 210009,China3 Institute of Cancer Research,Shanghai 200032,China | 2001 | World Journal of Gastroenterology2001，7，5： | 18 |
| 19 | Polyphenolic Compound and the Degree of Browning in Processing Apple Varieties显示文摘Polyphenolic compound in processing apple (Malus domestica Borkh.) varieties and the relationship between polyphenol content and enzymatic browning were studied to provide reference for raw material selection and processing method optimization. The content of polyphenol compound in 10 processing apple varieties (4 cider and 6 juice varieties) were analyzed using the Folin-Ciocalteu method and HPLC. The degree of browning and the activities of polyphenol oxidase were also studied. The content and proportion of the polyphenol varied depending on the variety. Bitter varieties globally showed a higher polyphenol concentration than sweet or acid varieties. Proanthocyanidins, chlorogenic acid, (+)-catechin, (-)-epicatechin were high-concentrated polyphenols in apple fruits. Phloridzin, the unique polyphenol of apple, was abundant in the bitter variety Frequin rouge fruit. Total polyphenols, proanthocyanidins, (+)-catechin, and phloridzin had higher correlations with browning. The correlation was low between chlorogenic acid and browning. The polyphenolic profiles were correlated with the apple types. Cider apples contained more polyphenol than juice apple varieties. The content of flavan-3-ol has a close relationship with fruit browning. | SONG Ye YAO Yu-xin ZHAI Heng DU Yuan-peng CHEN Feng WEI Shu-wei | 2007 | Agricultural Sciences in China2007，6，5： | 17 |
| 20 | Subcellular distribution and compartmentalization of arsenic in Pteris vittata L.显示文摘The subcellular distribution of arsenic (As) in Pteris vittata L., an As-hyperaccumulator, was studied to de- termine As compartmentalization and to explore the mecha- nisms that confer As tolerance. When the plant was grown in a nutrient solution without additional As, most of the accu- mulated As was isolated to the cell wall. However, in plants growing in a nutrient solution containing 0.1 or 0.2 mmol/L As, approximately 78% of the total As accumulated within the pinna. The proportions of As accumulation in the cyto- plasmic supernatant fraction were 78% of that in the pinna and 61% of that in the plant. In either treatment group (0.1 or 0.2 mmol/L As), the fraction containing the lowest level of As was the organelle fraction. These results suggest that As accumulates in the pinna where it is primarily distributed in the cytoplasmic supernatant fraction. The role of As com- partmentalization may be intricately linked with As detoxi- fication in P. vittata L. | CHEN Tongbin YAN Xiulan LIAO Xiaoyong XIAO Xiyuan HUANG Zechun XIE Hua ZHAI Limei | 2005 | Chinese Science Bulletin2005，50，24： | 17 |
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