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| 1 | Acupuncture and regulation of gastrointestinal function显示文摘In China, acupuncture has been considered an effective method for treating gastrointestinal(GI) dysfunction diseases for thousands of years. In fact, acupuncture has gained progressive acceptance from both practitioners and patients worldwide. However, the therapeutic effects and underlying mechanisms in treating GI dysfunction have not yet been established due to a lack of systematic and comprehensive review articles. Therefore, the aim of this review is to discuss the efficacy of acupuncture as a treatment for GI dysfunction and the associated underlying mechanisms. A search of Pub Med was conducted for articles that were published over the past 10 years using the terms 'acupuncture', 'gastrointestine', and other relevant keywords. In the following review, we describe the effect and underlying mechanisms of acupuncture on GI function from the perspectives of GI motility, visceral sensitivity, the GI barrier, and the brain-gut axis. The dual regulatory effects of acupuncture may manifest by promoting gastric peristalsis in subjects with low initial gastric motility, and suppressing peristalsis in subjects with active initial motility. In addition, the regulation of acupuncture on gastric motility may be intensitydependent. Our findings suggest that further studies are needed to investigate the effects and more systematic mechanisms in treating GI dysfunction, and to promote the application of acupuncture for the treatment of GI diseases. | Hui Li Tian He Qian Xu Zhe Li Yan Liu Fang Li Bo-Feng Yang Cun-Zhi Liu | 2015 | World Journal of Gastroenterology2015，21，27： | 41 |
| 2 | Effect of Boschniakia rossica on expression of GSTP,p53 and p21^(ras)proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats显示文摘AIM To investigate the effect of Boschniakiarossica(BR)extract on expression of GST-P,p53 and p21rasproteins in early stage of chemicalhepatocarcinogenesis in rats and its anti-inflammatory activities.METHODS The expression of tumor marker-placental form glutathione S-transferase(GST-P),p53 and p21rasproteins were investigated byimmunohistochemical techniques and ABCmethod.Anti-inflammatory activities of BR werestudied by xylene and croton oil-induced mouseear edema,carrageenin,histamine and hotscald-induced rat pow edema,adjuvant-inducedrat arthritis and cotton pellet-induced mousegranuloma formation methods.RESULTS The 500 mg/kg of BR-H2O extractfractionated from BR-Methanol extract hadinhibitory effect on the formation of DEN-inducedGST-P-positive foci in rat liver(GST-P stainingwas 78% positive in DEN+AAF group vs 20%positive in DEN+AAF+BR group,P<0.05)andthe expression of mutant p53 and p21rasproteinwas lower than that of hepatic preneoplasticlesions(33% and 22% positive respectively inDEN+AAF group vs negative in DEN+AAF+BRgroup).Both CH2Cl2 and H2O extracts from BRhad anti-inflamatory effect in xylene and crotonoil-induced mouse ear edema(inhibitory rateswere 26%-29% and 35%-59%,respectively). BR-H2O extract exhibited inhibitory effect incarrageenin,histamine and hot scald-inducedhind paw edema and adjuvant-induced arthritis inrats and cotton pellet-induced granulomaformation in mice.CONCLUSION BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical hepato-carcinogenesis. Both CH2CI2 and H2O extracts from BR exerted anti-inflammatory effect in rats and mice. | Zong Zhu Yin Hai Ling Jin Xue Zhe Yin Tian Zhu Li Ji Shu Quan Zeng Nan Jin Institute for Cancer Research,Yanbian University College of Medicine,Yanji 133000,Jilin Province,China | 2000 | World Journal of Gastroenterology2000，6，6： | 33 |
| 3 | Pathogenicity of a currently circulating Chinese variant pseudorabies virus in pigs显示文摘AIM:To test the pathogenicity of pseudorabies virus(PRV)variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.METHODS:The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by different inoculating routes,virus loads,and ages of pigs.The classical PRV Fa strain was then used to compare with HN1201 to determine pathogenicity.Clinical symptoms after virus infection were recorded daily and average daily body weight was used to measure the growth performance of pigs.At necropsy,gross pathology and histopathology were used to evaluate the severity of tissue damage caused by virus infection.RESULTS:The results showed that the efficient infection method of RPV HN1201 was via intranasal inoculation at 107 TCID50,and that the virus has high pathogenicity to 35-to 127-d old pigs.Compared with Fa strain,pigs infected with HN1201 showed more severe clinical symptoms and pathological lesions.Immunochemistry results revealed HN1201 had more abundant antigen distribution in extensive organs.CONCLUSION:All of the above results suggest that PRV variant HN1201 was more pathogenic to pigs than the classical Fa strain. | Qing-Yuan Yang Zhe Sun Fei-Fei Tan Ling-Hua Guo Yu-Zhou Wang Juan Wang Zhi-Yan Wang Li-Lin Wang Xiang-Dong Li Yan Xiao Ke-Gong Tian | 2016 | World Journal of Virology2016，5，1： | 31 |
| 4 | m^6A-dependent biogenesis of circular RNAs in male germ cells显示文摘The majority of circular RNAs(circRNAs)spliced from coding genes contain open reading frames(ORFs)and thus,have protein coding potential.However,it remains unknown what regulates the biogenesis of these ORF-containing circRNAs,whether they are actually translated into proteins and what functions they play in specific physiological contexts.Here,we report that a large number of circRNAs are synthesized with increasing abundance when late pachytene spermatocytes develop into round and then elongating spermatids during murine spermatogenesis.For a subset of circRNAs,the back splicing appears to occur mostly at m^6A-enriched sites,which are usually located around the start and stop codons in linear mRNAs.Consequently,approximately a half of these male germ cell circRNAs contain large ORFs with m^6A-modified start codons in their junctions,features that have been recently shown to be associated with protein-coding potential.Hundreds of peptides encoded by the junction sequences of these circRNAs were detected using liquid chromatography coupled with mass spectrometry,suggesting that these circRNAs can indeed be translated into proteins in both developing(spermatocytes and spermatids)and mature(spermatozoa)male germ cells.The present study discovered not only a novel role of m^6A in the biogenesis of coding circRNAs,but also a potential mechanism to ensure stable and long-lasting protein production in the absence of linear mRNAs,i.e.,through production of circRNAs containing large ORFs and m^6A-modified start codons in junction sequences. | Chong Tang Yeming Xie Tian Yu Na Liu Zhuqing Wang Rebekah J.Woolsey Yunge Tang Xinzong Zhang Weibing Qin Ying Zhang Ge Song Weiwei Zheng Juan Wang Weitian Chen Xiongyi Wei Zhe Xie Rachel Klukovich Huili Zheng David R.Quilici Wei Yan | 2020 | Cell Research2020，30，3： | 12 |
| 5 | Aerodynamic map for soft and hard hypersonic level flight in near space显示文摘In this note,we design a velocity-altitude map forhypersonic level flight in near space of altitude 20-100 km.This map displays aerodynamic-related parameters associ-ated with near space level flight,schematically or quantita-tively.Various physical conditions for the near-space levelflight are then characterized,including laminar or turbulentflow,rarefaction or continuous flow,aerodynamic heating,aswell as conditions for sustaining level flight with and withoutorbital effect.This map allows one to identify conditions tohave soft flight or hard flight,and this identification wouldbe helpful for making correct planning on detailed studies ofaerodynamics or making initial design of near space vehicles. | Ruifeng Hu Ziniu Wu Zhe Wu Xiaoxin Wang Zhongwei Tian School of Aerospace, Tsinghua University, 100084 Beijing, China | 2009 | Acta Mechanica Sinica2009，25，4： | 11 |
| 6 | Monitoring of 943 organic micropollutants in wastewater from municipal wastewater treatment plants with secondary and advanced treatment processes显示文摘To perform a systematic survey on the occurrence and removal of micropollutants during municipal wastewater treatment, 943 semi-volatile organic chemicals in 32 wastewater samples including influents of secondary treatments, secondary effluents and final effluents(effluents of advanced treatments), which were collected from seven full-scale municipal wastewater treatment plants(MWTPs) in China, were examined by gas chromatography-mass spectrometry(GC-MS) coupled with an automated identification and quantification system with a database(AIQS-DB). In total, 196 and 145 chemicals were detected in secondary and final effluents, respectively. The majority of the total concentrations(average removal efficiency, 87.0%±5.9%) of the micropollutants were removed during secondary treatments. However, advanced treatments achieved different micropollutant removal extents from secondary effluents depending on the different treatment processes employed. Highly variable removal efficiencies of total concentrations(32.7%–99.3%) were observed among the different advanced processes. Among them,ozonation-based processes could remove 70.0%–80.9% of the total concentrations of studied micropollutants. The potentially harmful micropollutants, based on their detection frequency and concentration in secondary and final effluents, were polycyclic aromatic hydrocarbons(PAHs)(2-methylnaphthalene, fluoranthene, pyrene, naphthalene and phenanthrene), phosphorus flame retardants(tributyl phosphate(TBP), tris(2-chloroethyl)phosphate(TCEP) and tris(1,3-dichloro-2-propyl) phosphate(TDCP)), phthalates(bis(2-ethylhexyl)phthalate(DEHP)), benzothiazoles(benzothiazole,2-(methylthio)-benzothiazol, and 2(3H)-benzothiazolone) and phenol. This study indicated that the presence of considerable amounts of micropollutants in secondary effluent creates the need for suitable advanced treatment before their reuse. | Juan Wang Zhe Tian Yingbin Huo Min Yang Xingcan Zheng Yu Zhang | 2018 | Journal of Environmental Sciences2018，30，5： | 9 |
| 7 | Impact of treatment modalities on patients with recurrent hepatocellular carcinoma after liver transplantation:Preliminary experience显示文摘Background:Post-liver transplantation(LT)hepatocellular carcinoma(HCC)recurrence still occurs in approximately 20%of patients and drastically affects their survival.This study aimed to evaluate the efficacy of various treatments for recurrent HCC after LT in a Chinese population.Methods:A total of 64 HCC patients with tumor recurrence after LT were enrolled in this study.Univariate and multivariate analyses were performed to identify factors affecting post-recurrence survival.Results:Of the 64 patients with recurrent HCC after LT,those who received radical resection followed by nonsurgical therapy had a median overall survival(OS)of 20.9 months after HCC recurrence,significantly superior to patients who received only nonsurgical therapy(9.4 months)or best supportive care(2.4 months).The one-and two-year OS following recurrence was favorable for patients receiving radical resection followed by nonsurgical therapy(93.8%,52.6%),poor for patients receiving only nonsurgical therapy(30.8%,10.8%),and dismal for patients receiving best supportive care(0%,0%;overall P<0.001).Median OS in sorafenib-tolerant patients treated with lenvatinib was 19.5 months,far surpassing the patients that discontinued sorafenib or were treated with regorafenib after sorafenib failure(12 months,P<0.001).Compared with tacrolimus-based immunosuppressive therapy,OS was significantly increased with sirolimus-based therapy at one and two years after HCC recurrence(P=0.035).Multivariate analysis showed radical resection combined with nonsurgical therapy for recurrent HCC and sorafenib-lenvatinib sequential therapy were independent favorable factors for post-recurrence survival.Conclusions:Aggressive surgical intervention in well-selected patients significantly improves OS after recurrence.A multidisciplinary treatment approach is required to slow down disease progression for patients with unresectable recurrent HCC. | Zhe Yang Shuo Wang Xin-Yao Tian Qin-Fen Xie Li Zhuang Qi-Yong Li Cheng-Ze Chen Shu-Sen Zheng | 2020 | Hepatobiliary & Pancreatic Diseases International2020，19，4： | 9 |
| 8 | Cutting-balloon angioplasty before drug-eluting stent implantation for the treatment of severely calcified coronary lesions显示文摘BackgroundSeverely 钙化的冠的损害对汽球 angioplasty 糟糕作出回应,导致不完全、不均匀的 stent 扩大。因此,在 drug-eluting stent (DES ) 以前的足够的匾修正培植是为钙化的损害治疗的钥匙。这研究是评估为严重地钙化的冠的 lesions.MethodsNinety 切汽球 angioplasty 的安全和功效 -- 有严重地钙化的损害的二个连续病人(定义为钙弧 ≥180 °钙长度比率 ≥0.5 ) 在 DES 培植随机基于汽球类型被划分成二个组以前,与汽球膨胀对待:在常规汽球 angioplasty (BA ) 的 45 个病人组织,在切的汽球 angioplasty (CB ) 的 47 个病人组织。在 BA 组的七个盒子令人满意地没完成膨胀并且被变成 CB 组。 Intravascular 超声( IVUS )在汽球膨胀前并且在 stent 培植以后被执行获得质、量的损害特征并且评估 stent ,包括最小的腔代表性的区域( CSA ),钙化的弧和长度,最小的 stent CSA , stent 并置, stent 对称, stent 扩大,容器解剖,并且分叉容器监狱。在里面医院, 1 月、 6 月的主要不利心脏的事件(向) 是 reported.ResultsThere 不是在在二个组之间的临床的特征的统计差别,包括钙弧(222.2 °±22.2 °对 235.0 °±22.1 °, P = 0.570 ) ,钙长度比率(0.67 ±0.06 对 0.77 ±0.05, P = 0.130 ) ,并且在一种总线标准前的最小的腔 CSA (2.59 ±0.08 公里 2 对 2.52 ±0.08 公里 2, P = 0.550 ) 。在 stent 培植以后,最后的最小的 stent CSA (6.26 ±0.40 公里 2 对 5.03 ±0.33 公里 2 ;P = 0.031 ) 并且尖锐的腔获得(3.74 ±0.38 公里 2 对 2.44 ±0.29 公里 2, P = 0.015 ) 比 BA 组的在 CB 组是显著地更大的。没有统计上在在二个组之间的 stent 扩大, stent 对称,不完全的 stent 并置,容器解剖和分支容器监狱的差别。在在严重地钙化的损害的 DES 培植看起来是包括显著地更大的最后的 stent CSA 和更大的尖锐的腔获得的更多的功效以前, 30 天、 6 月的向率也不是 different.ConclusionsCutting 汽球 angioplasty,没有增加在操作和 MACE 率在期间的复杂并发症 6 月。 | Zhe TANG Jing BAI Shao-Ping SU Yu WANG Mo-Han LIU Qi-Cai BAI Jin-Wen TIAN Qiao XUE Lei GAO Chun-Xiu AN Xiao-Juan LIU | 2014 | Journal of Geriatric Cardiology2014，11，1： | 9 |
| 9 | Controllable optical activity with non-chiral plasmonic metasurfaces显示文摘Optical activity is the rotation of the plane of linearly polarized light along the propagation direction as the light travels through optically active materials.In existing methods,the strength of the optical activity is determined by the chirality of the materials,which is difficult to control quantitatively.Here we numerically and experimentally investigated an alternative approach to realize and control the optical activity with non-chiral plasmonic metasurfaces.Through judicious design of the structural units of the metasurfaces,the right and left circular polarization components of the linearly polarized light have different phase retardations after transmitting through the metasurfaces,leading to large optical activity.Moreover,the strength of the optical activity can be easily and accurately tuned by directly adjusting the phase difference.The proposed approach based on non-chiral plasmonic metasurfaces exhibits large optical activity with a high controllable degree of freedom,which may provide more possibilities for applications in photonics. | Ping Yu Jianxiong Li Chengchun Tang Hua Cheng Zhaocheng Liu Zhancheng Li Zhe Liu Changzhi Gu Junjie Li Shuqi Chen Jianguo Tian | 2016 | Light(Science & Applications)2016，5，1： | 8 |
| 10 | Interaction between Schwann cells and other cells during repair of peripheral nerve injury显示文摘Peripheral nerve injury(PNI)is common and,unlike damage to the central nervous system injured nerves can effectively regenerate depending on the location and severity of injury.Peripheral myelinating glia,Schwann cells(SCs),interact with various cells in and around the injury site and are important for debris elimination,repair,and nerve regeneration.Following PNI,Wallerian degeneration of the distal stump is rapidly initiated by degeneration of damaged axons followed by morphologic changes in SCs and the recruitment of circulating macrophages.Interaction with fibroblasts from the injured nerve microenvironment also plays a role in nerve repair.The replication and migration of injury-induced dedifferentiated SCs are also important in repairing the nerve.In particular,SC migration stimulates axonal regeneration and subsequent myelination of regenerated nerve fibers.This mobility increases SC interactions with other cells in the nerve and the exogenous environment,which influence SC behavior post-injury.Following PNI,SCs directly and indirectly interact with other SCs,fibroblasts,and macrophages.In addition,the inter-and intracellular mechanisms that underlie morphological and functional changes in SCs following PNI still require further research to explain known phenomena and less understood cell-specific roles in the repair of the injured peripheral nerve.This review provides a basic assessment of SC function post-PNI,as well as a more comprehensive evaluation of the literature concerning the SC interactions with macrophages and fibroblasts that can influence SC behavior and,ultimately,repair of the injured nerve. | Wen-Rui Qu Zhe Zhu Jun Liu De-Biao Song Heng Tian Bing-Peng Chen Rui Li Ling-Xiao Deng | 2021 | Neural Regeneration Research2021，16，1： | 8 |
| 11 | Three-dimensional bioprinted glioblastoma microenvironments model cellular dependencies and immune interactions显示文摘Brain tumors are dynamic complex ecosystems with multiple cell types.To model the brain tumor microenvironment in a reproducible and scalable system,we developed a rapid three-dimensional(3D)bioprinting method to construct clinically relevant biomimetic tissue models.In recurrent glioblastoma,macrophages/microglia prominently contribute to the tumor mass.To parse the function of macrophages in 3D,we compared the growth of glioblastoma stem cells(GSCs)alone or with astrocytes and neural precursor cells in a hyaluronic acid-rich hydrogel,with or without macrophage.Bioprinted constructs integrating macrophage recapitulate patient-derived transcriptional profiles predictive of patient survival,maintenance of stemness,invasion,and drug resistance.Whole-genome CRISPR screening with bioprinted complex systems identified unique molecular dependencies in GSCs,relative to sphere culture.Multicellular bioprinted models serve as a scalable and physiologic platform to interrogate drug sensitivity,cellular crosstalk,invasion,context-specific functional dependencies,as well as immunologic interactions in a species-matched neural environment. | Min Tang Qi Xie Ryan C.Gimple Zheng Zhong Trevor Tam Jing Tian Reilly L.Kidwell Qiulian Wu Briana C.Prager Zhixin Qiu Aaron Yu Zhe Zhu Pinar Mesci Hui Jing Jacob Schimelman Pengrui Wang Derrick Lee Michael H.Lorenzini Deobrat Dixit Linjie Zhao Shruti B.hargava Tyler E.Miller Xueyi Wan Jing Tang Bingjie Sun Benjamin F.Cravatt Alysson R.Muotri Shaochen Chen Jeremy N.Rich | 2020 | Cell Research2020，30，10： | 6 |
| 12 | Gut microbial balance and liver transplantation: alteration, management, and prediction显示文摘肝移植是为终端阶段肝疾病的常规治疗。然而,几复杂并发症仍然妨碍幸存率。肠的障碍破坏广泛地在收到肝的病人之中被观察移植并且因为在肠和肝之间的关系,受不了 ischemiareperfusion 或拒绝损害,两个在解剖和功能。重要地,内脏 microbiota 的产生改变在这个过程期间加重接枝机能障碍。这篇文章为内脏考察研究进步微生物引起的改变和肝移植。特别,这个工作也在内脏的管理上评估研究微生物引起的改变和在肝移植期间利用微生物引起的改变的可能的损害的预言。另外,我们为关于的研究建议可能的方向在肝移植期间毁坏微生物引起的改变并且在肝移植在微生物引起的改变的利用上提供一个假设。目的是不仅预言 perioperative 损害而且作为治疗的一个方法工作或甚至禁止肝移植的拒绝。 | Xinyao Tian Zhe Yang Fangzhou Luo Shusen Zheng | 2018 | Frontiers of Medicine2018，12，2： | 5 |
| 13 | DeST 3.0:A new-generation building performance simulation platform显示文摘Buildings contribute to almost 30%of total energy consumption worldwide.Developing building energy modeling programs is of great significance for lifecycle building performance assessment and optimization.Advances in novel building technologies,the requirements of high-performance computation,and the demands for multi-objective models have brought new challenges for building energy modeling software and platforms.To meet the increasing simulation demands,DeST 3.0,a new-generation building performance simulation platform,was developed and released.The structure of DeST 3.0 incorporates four simulation engines,including building analysis and simulation(BAS)engine,HVAC system engine,combined plant simulation(CPS)engine,and energy system(ES)engine,connected by air loop and water loop balancing iterations.DeST 3.0 offers numerous new simulation features,such as advanced simulation modules for building envelopes,occupant behavior and energy systems,cross-platform and compatible simulation kernel,FMI/FMU-based co-simulation functionalities,and high-performance parallel simulation architecture.DeST 3.0 has been thoroughly evaluated and validated using code verification,inter-program comparison,and case-study calibration.DeST 3.0 has been applied in various aspects throughout the building lifecycle,supporting building design,operation,retrofit analysis,code appliance,technology adaptability evaluation as well as research and education.The new generation building simulation platform DeST 3.0 provides an efficient tool and comprehensive simulation platform for lifecycle building performance analysis and optimization. | Da Yan Xin Zhou Jingjing An Xuyuan Kang Fan Bu Youming Chen Yiqun Pan Yan Gao Qunli Zhang Hui Zhou Kuining Qiu Jing Liu Yan Liu Honglian Li Lei Zhang Hong Dong Lixin Sun Song Pan Xiang Zhou Zhe Tian Wenjie Zhang Ruhong Wu Hongsan Sun Yu Huang Xiaohong Su Yongwei Zhang Rui Shen Diankun Chen Guangyuan Wei Yixing Chen Jinqing Peng | 2022 | Building Simulation2022，15，11： | 5 |
| 14 | Acoustic impedance inversion of zero-offset VSP data显示文摘高度精确的声学的阻抗倒置是为预先钻的一种关键技术由 VSP 数据的预言。在这篇论文,基于 VSP 数据有高分辨率的事实,高发信号到噪音比率,并且下面去并且在去上面,波浪能精确地被分开,我们建议用 VSP 数据在位前面在地上凿穿下面预言阻抗的一个方法。首先,非线性的反复的倒置的方法被采用用 VSP 走廊栈为阻抗转换。然后,由在重复并且用 preconditioned 修改抑制因素结合坡度方法解决倒置结果的方程,稳定性和集中能被提高。理论模型和实际数据的结果证明方法为预先钻是有效的预言使用 VSP 数据。 | Wang Jing Liu Yang Sun Zhe Tian Hong Su Hua Zhao Qianhua Liu Yingyu | 2009 | Applied Geophysics2009，6，2： | 5 |
| 15 | An improved fruit fly optimization algorithm for solving traveling salesman problem显示文摘The traveling salesman problem(TSP), a typical non-deterministic polynomial(NP) hard problem, has been used in many engineering applications. As a new swarm-intelligence optimization algorithm, the fruit fly optimization algorithm(FOA) is used to solve TSP, since it has the advantages of being easy to understand and having a simple implementation. However, it has problems, including a slow convergence rate for the algorithm, easily falling into the local optimum, and an insufficient optimization precision. To address TSP effectively, three improvements are proposed in this paper to improve FOA. First, the vision search process is reinforced in the foraging behavior of fruit flies to improve the convergence rate of FOA. Second, an elimination mechanism is added to FOA to increase the diversity. Third, a reverse operator and a multiplication operator are proposed. They are performed on the solution sequence in the fruit fly's smell search and vision search processes, respectively. In the experiment, 10 benchmarks selected from TSPLIB are tested. The results show that the improved FOA outperforms other alternatives in terms of the convergence rate and precision. | Lan HUANG Gui-chao WANG Tian BAI Zhe WANG | 2017 | Frontiers of Information Technology & Electronic Engineering2017，18，10： | 4 |
| 16 | Genetic characterization and potential molecular dissemination mechanism of tet(31) gene in Aeromonas caviae from an oxytetracycline wastewater treatment system显示文摘Recently, the rarely reported tet(31) tetracycline resistance determinant was commonly found in Aeromonas salmonicida, Gallibacterium anatis, and Oblitimonas alkaliphila isolated from farming animals and related environment. However, its distribution in other bacteria and potential molecular dissemination mechanism in environment are still unknown. The purpose of this study was to investigate the potential mechanism underlying dissemination of tet(31) by analysing the tet(31)-carrying fragments in A. caviae strains isolated from an aerobic biofilm reactor treating oxytetracycline bearing wastewater. Twenty-three A. caviae strains were screened for the tet(31) gene by polymerase chain reaction(PCR). Three strains(two harbouring tet(31), one not) were subjected to whole genome sequencing using the PacBio RSII platform. Seventeen A. caviae strains carried the tet(31) gene and exhibited high resistance levels to oxytetracycline with minimum inhibitory concentrations(MICs)ranging from 256 to 512 mg/L. tet(31) was comprised of the transposon Tn6432 on the chromosome of A. caviae, and Tn6432 was also found in 15 additional tet(31)-positive A. caviae isolates by PCR. More important, Tn6432 was located on an integrative conjugative element(ICE)-like element, which could mediate the dissemination of the tet(31)-carrying transposon Tn6432 between bacteria. Comparative analysis demonstrated that Tn6432 homologs with the structure ISCR2-ΔphzF-tetR(31)-tet(31)-ΔglmM-sul2 were also carried by A. salmonicida, G. anatis, and O. alkaliphila, suggesting that this transposon can be transferred between species and even genera. This work provides the first report on the identification of the tet(31) gene in A. caviae, and will be helpful in exploring the dissemination mechanisms of tet(31) in water environment. | Yanhong Shi Zhe Tian Sébastien Olivier Leclercq Hong Zhang Min Yang Yu Zhang | 2019 | Journal of Environmental Sciences2019，31，2： | 4 |
| 17 | Association between Fine Particulate Air Pollution and Outpatient Visits for Eczema in Beijing, China: A Time-series Analysis显示文摘Eczema is a very common inflammatory skin disease characterized by patches of erythematous,itchy, cracked, and scaly skin.According to the 2010 Global Burden of Disease Study, the global prevalence of eczema is 3.33% and the leading cause of skin condition-based disability-adjusted life years is eczema[1].The large health burden brought about by eczema suggests high direct medical costs and health care utilization[2].Thus, eczema prevention is of great importance in efforts to eliminate the public health burden of the disease. | WANG Xiao Wen TIAN Yao Hua CAO Ya Ying SONG Jing LI Man WU Yao WANG Meng Ying HUANG Zhe HU Yong Hua | 2019 | Biomedical and Environmental Sciences2019，32，8： | 4 |
| 18 | Genome-wide identification of agronomically important genes in outcrossing crops using OutcrossSeq显示文摘Many important crops(e.g.,tuber,root,and tree crops)are cross-pollinating.For these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid genome.In this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing populations.We applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic traits.Candidate causative genes at these loci were detected based on functional clues.Taken together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient way.The OutcrossSeq software and its instruction manual are available for downloading at www.xhhuanglab.cn/tool/OutcrossSeq.html. | Mengjiao Chen Weijuan Fan Feiyang Ji Hua Hua Jie Liu Mengxiao Yan Qingguo Ma Jiongjiong Fan Qin Wang Shufeng Zhang Guiling Liu Zhe Sun Changgeng Tian Fengling Zhao Jianli Zheng Qi Zhang Jiaxin Chen Jie Qiu Xin Wei Ziru Chen Peng Zhang Dong Pei Jun Yang Xuehui Huang | 2021 | Molecular Plant2021，14，4： | 4 |
| 19 | Synergistic anti-liver cancer effects of curcumin and total ginsenosides显示文摘BACKGROUND Liver cancer is the sixth most frequently occurring cancer in the world and the fourth most common cause of cancer mortality.The pathogenesis of liver cancer is closely associated with inflammation and immune response in the tumor microenvironment.New therapeutic agents for liver cancer,which can control inflammation and restore cellular immunity,are required.Curcumin(Cur)is a natural anti-inflammatory drug,and total ginsenosides(TG)are a commonly used immunoregulatory drug.Of note,both Cur and TG have been shown to exert anti-liver cancer effects.AIM To determine the synergistic immunomodulatory and anti-inflammatory effects of Cur combined with TG in a mouse model of subcutaneous liver cancer.METHODS A subcutaneous liver cancer model was established in BALB/c mice by a subcutaneous injection of hepatoma cell line.Animals were treated with Cur(200 mg/kg per day),TG(104 mg/kg per day or 520 mg/kg per day),the combination of Cur(200 mg/kg per day)and TG(104 mg/kg per day or 520 mg/kg per day),or 5-fluorouracil combined with cisplatin as a positive control for 21 d.Tumor volume was measured and the protein expression of programmed cell death 1 and programmed cell death 1 ligand 1(PD-L1),inflammatory indicators Toll like receptor 4(TLR4)and nuclear factor-κB(NF-κB),and vascular growth-related factors nitric oxide synthases(iNOS)and matrix metalloproteinase 9 were analyzed by Western blot analysis.CD4+CD25+Foxp3+regulatory T cells(Tregs)were counted by flow cytometry.RESULTS The combination therapy of Cur and TG significantly inhibited the growth of liver cancer,as compared to vehicle-treated animals,and TG showed dose dependence.Cur combined with TG-520 markedly decreased the protein expression of PD-L1(P<0.0001),while CD4+CD25+Foxp3+Tregs regulated by the PD-L1 signaling pathway exhibited a positive correlation with PD-L1.Cur combined with TG-520 also inhibited the cascade action mediated by NF-κB(P<0.0001),thus inhibiting the TLR4/NF-κB signalling pathway(P=0.0088,P<0.0001),which is associated with inflammation and acts on PD-L1.It also inhibited the NF-κB-MMP9 signalling pathway(P<0.0001),which is associated with tumor angiogenesis.CONCLUSION Cur combined with TG regulates immune escape through the PD-L1 pathway and inhibits liver cancer growth through NF-κB-mediated inflammation and angiogenesis. | Zhe Deng Xiao-Yan Xu Fenny Yunita Qing Zhou Yong-Rong Wu Yu-Xing Hu Zhi-Qi Wang Xue-Fei Tian | 2020 | World Journal of Gastrointestinal Oncology2020，12，10： | 4 |
| 20 | Characterization of the Hot Deformation Behavior of Cu–Cr–Zr Alloy by Processing Maps显示文摘Hot deformation behavior of the Cu–Cr–Zr alloy was investigated using hot compressive tests in the temperature range of 650–850 °C and strain rate range of 0.001–10 s-1. The constitutive equation of the alloy based on the hyperbolic-sine equation was established to characterize the flow stress as a function of strain rate and deformation temperature. The critical conditions for the occurrence of dynamic recrystallization were determined based on the alloy strain hardening rate curves. Based on the dynamic material model, the processing maps at the strains of 0.3, 0.4 and 0.5were obtained. When the true strain was 0.5, greater power dissipation efficiency was observed at 800–850 °C and under0.001–0.1 s-1, with the peak efficiency of 47%. The evolution of DRX microstructure strongly depends on the deformation temperature and the strain rate. Based on the processing maps and microstructure evolution, the optimal hot working conditions for the Cu–Cr–Zr alloy are in the temperature range of 800–850 °C and the strain rate range of 0.001–0.1 s-1. | Yi Zhang Hui-Li Sun Alex A.Volinsky Bao-Hong Tian Zhe Chai Ping Liu Yong Liu | 2016 | Acta Metallurgica Sinica(English Letters)2016，29，5： | 3 |
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