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| 1 | Three-dimensional bioprinting of multicell-laden scaffolds containing bone morphogenic protein-4 for promoting M2 macrophage polarization and accelerating bone defect repair in diabetes mellitus显示文摘Critical-sized bone defect repair in patients with diabetes mellitus remains a challenge in clinical treatment because of dysfunction of macrophage polarization and the inflammatory microenvironment in the bone defect region.Three-dimensional(3D)bioprinted scaffolds loaded with live cells and bioactive factors can improve cell viability and the inflammatory microenvironment and further accelerating bone repair.Here,we used modified bioinks comprising gelatin,gelatin methacryloyl(GelMA),and 4-arm poly(ethylene glycol)acrylate(PEG)to fabricate 3D bioprinted scaffolds containing BMSCs,RAW264.7 macrophages,and BMP-4-loaded mesoporous silica nanoparticles(MSNs).Addition of MSNs effectively improved the mechanical strength of GelMA/gelatin/PEG scaffolds.Moreover,MSNs sustainably released BMP-4 for long-term effectiveness.In 3D bioprinted scaffolds,BMP-4 promoted the polarization of RAW264.7 to M2 macrophages,which secrete anti-inflammatory factors and thereby reduce the levels of pro-inflammatory factors.BMP-4 released from MSNs and BMP-2 secreted from M2 macrophages collectively stimulated the osteogenic differentiation of BMSCs in the 3D bioprinted scaffolds.Furthermore,in calvarial critical-size defect models of diabetic rats,3D bioprinted scaffolds loaded with MSNs/BMP-4 induced M2 macrophage polarization and improved the inflammatory microenvironment.And 3D bioprinted scaffolds with MSNs/BMP-4,BMSCs,and RAW264.7 cells significantly accelerated bone repair.In conclusion,our results indicated that implanting 3D bioprinted scaffolds containing MSNs/BMP-4,BMSCs,and RAW264.7 cells in bone defects may be an effective method for improving diabetic bone repair,owing to the direct effects of BMP-4 on promoting osteogenesis of BMSCs and regulating M2 type macrophage polarization to improve the inflammatory microenvironment and secrete BMP-2. | Xin Sun Zhenjiang Ma Xue Zhao Wenjie Jin Chenyu Zhang Jie Ma Lei Qiang Wenhao Wang Qian Deng Han Yang Jinzhong Zhao Qianqian Liang Xiaojun Zhou Tao Li Jinwu Wang | 2021 | Bioactive Materials2021，6，3： | 12 |
| 2 | Abscisic acid and ethephon regulation of cellulase in the endosperm cap and radicle during lettuce seed germination显示文摘The purpose of this study was to investigate the role of cellulase in endosperm cap weakening and radicle elongation during lettuce(Lactuca sativa L.) seed germination. The application of abscisic acid(ABA) or ethephon inhibits or promotes germination, respectively, by affecting endosperm cap weakening and radicle elongation. Cellulase activities, and related protein and transcript abundances of two lettuce cellulase genes, Ls CEL1 and Ls CEL2, increase in the endosperm cap and radicle prior to radicle protrusion following imbibition in water. ABA or ethephon reduce or elevate, respectively,cellulase activity, and related protein and transcript abundances in the endosperm cap. Taken together, these observations suggest that cellulase plays a role in endosperm cap weakening and radicle elongation during lettuce seed germination, andthat the regulation of cellulase in the endosperm cap by ABA and ethephon play a role in endosperm cap weakening.However, the influence of ABA and ethephon on radicle elongation may not be through their effects on cellulase. | Bingxian Chen Jun Ma Zhenjiang Xu Xiaofeng Wang | 2016 | Journal of Integrative Plant Biology2016，58，10： | 6 |
| 3 | Preparation and characterization of long chain branched polypropylene mediated by different heteroaromatic ring derivatives显示文摘 | Dong Wan Li Ma Haiping Xing Lu Wang Zhenjiang Zhang Jian Qiu Guangchun Zhang Tao Tang | 2012 | Polymer2012，，： | 1 |
| 4 | Preparation of titania/carbon nanotube composites using supercritical ethanol and their photocatalytic activity for phenol degradation under visible light irradiation显示文摘 | Guimin An Wanhong Ma Zhenyu Sun Zhimin Liu Buxing Han Shiding Miao Zhenjiang Miao Kunlun Ding | 2007 | Carbon2007，，9： | 1 |
| 5 | Synthesis and characterization of mesoporous aluminosilicate molecular sieve from K-feldspar显示文摘 | Shiding Miao Zhimin Liu Hongwen Ma Buxing Han Jimin Du Zhenyu Sun Zhenjiang Miao | 2005 | Microporous and Mesoporous Materials2005，，： | 1 |
| 6 | Controlling Degradation and Branching Reactions of Polypropylene by Different Heteroaromatic Ring Derivatives 显示文摘 | Dong Wan Li Ma Zhenjiang Zhang | 2012 | Polymer Degradation and Stability2012，97，1： | 1 |
| 7 | Con- trolling degradation and branching reactions of polypropylene by different heteroaromatic ring derivatives显示文摘 | Wan Dong Ma Li Zhang Zhenjiang | 2012 | Polymer Degradation and Stabil- ity2012，97，1： | 1 |
| 8 | Research of mini carhanding stability based on ADAMS显示文摘 | MA Zhenjiang CHEN Lin XU Ning | 2011 | Key EngineeringMateriais2011，486，4： | 1 |
| 9 | Controlling melt reactions during preparing long chain branched polypropylene using copper N , N -dimethyldithiocarbamate显示文摘 | Zhenjiang Zhang Haiping Xing Jian Qiu Zhiwei Jiang Haiou Yu Xiaohua Du Yanhui Wang Li Ma Tao Tang | 2010 | Polymer2010，，7： | 1 |
| 10 | Regulated macrophage immune microenvironment in 3D printed scaffolds for bone tumor postoperative treatment显示文摘The 3D printing technique is suitable for patient-specific implant preparation for bone repair after bone tumor resection.However,improving the survival rate due to tumor recurrence remains a challenge for implants.The macrophage polarization induction to M2-type tumor-associated macrophages(TAMs)by the tumor microenvironment is a key factor of immunosuppression and tumor recurrence.In this study,a regenerative scaffold regulating the macrophage immune microenvironment and promoting bone regeneration in a dual-stage process for the postoperative treatment of bone tumors was constructed by binding a colony-stimulating factor 1 receptor(CSF-1R)inhibitor GW2580 onto in situ cosslinked hydroxybutylchitosan(HBC)/oxidized chondroitin sulfate(OCS)hydrogel layer covering a 3D printed calcium phosphate scaffold based on electrostatic interaction.The hydrogel layer on scaffold surface not only supplied abundant sulfonic acid groups for stable loading of the inhibitor,but also acted as the cover mask protecting the bone repair part from exposure to unhealthy growth factors in the microenvironment at the early treatment stage.With local prolonged release of inhibitor being realized via the functional material design,CSF-1R,the main pathway that induces polarization of TAMs,can be efficiently blocked,thus regulating the immunosuppressive microenvironment and inhibiting tumor development at a low therapeutic dose.At the later stage of treatment,calcium phosphate component of the scaffold can facilitate the repair of bone defects caused by tumor excision.In conclusion,the difunctional 3D printed bone repair scaffold regulating immune microenvironment in stages proposed a novel approach for bone tumor postoperative treatment. | Cuidi Li Changwei Li Zhenjiang Ma Hongfang Chen Huitong Ruan Lianfu Deng Jinwu Wang Wenguo Cui | 2023 | Bioactive Materials2023，，1： | 1 |
| 11 | Bioprinted constructs that simulate nerve-bone crosstalk to improve microenvironment for bone repair显示文摘Crosstalk between nerves and bone is essential for bone repair,for which Schwann cells(SCs)are crucial in the regulation of the microenvironment.Considering that exosomes are critical paracrine mediators for intercellular communication that exert important effects in tissue repair,the aim of this study is to confirm the function and molecular mechanisms of Schwann cell-derived exosomes(SC-exos)on bone regeneration and to propose engineered constructs that simulate SC-mediated nerve-bone crosstalk.SCs promoted the proliferation and differentiation of bone marrow mesenchymal stem cells(BMSCs)through exosomes.Subsequent molecular mechanism studies demonstrated that SC-exos promoted BMSC osteogenesis by regulating the TGF-βsignaling pathway via let-7c-5p.Interestingly,SC-exos promoted the migration and tube formation performance of endothelial progenitor cells.Furthermore,the SC-exos@G/S constructs were developed by bioprinting technology that simulated SC-mediated nerve-bone crosstalk and improved the bone regeneration microenvironment by releasing SC-exos,exerting the regulatory effect of SCs in the microenvironment to promote innervation,vascularization,and osteogenesis and thus effectively improving bone repair in a cranial defect model.This study demonstrates the important role and underlying mechanism of SCs in regulating bone regeneration through SC-exos and provides a new engineered strategy for bone repair. | Tianchang Wang Wentao Li Yuxin Zhang Xiang Xu Lei Qiang Weiqiang Miao Xiaokun Yue Xin Jiao Xianhao Zhou Zhenjiang Ma Shuai Li Muliang Ding Junfeng Zhu Chi Yang Hui Wang Tao Li Xin Sun Jinwu Wang | 2023 | Bioactive Materials2023，，9： | 1 |
| 12 | Incorporating mesopelagic fish into the evaluation of conservation areas for marine living resources under climate change scenarios显示文摘Mesopelagic fish(meso-fish)are central species within the Southern Ocean(SO).However,their ecosystem role and adaptive capacity to climate change are rarely integrated into protected areas assessments.This is a pity given their importance as crucial prey and predators in food webs,coupled with the impacts of climate change.Here,we estimate the habitat distribution of nine meso-fish using an ensemble model approach(MAXENT,random forest,and boosted regression tree).Four climate model simulations were used to project their distribution under two representative concentration pathways(RCP4.5 and RCP8.5)for short-term(2006–2055)and long-term(2050–2099)periods.In addition,we assess the ecological representativeness of protected areas under climate change scenarios using meso-fish as indicator species.Our models show that all species shift poleward in the future.Lanternfishes(family Myctophidae)are predicted to migrate poleward more than other families(Paralepididae,Nototheniidae,Bathylagidae,and Gonostomatidae).In comparison,lanternfishes were projected to increase habitat area in the eastern SO but lose area in the western SO;the opposite was projected for species in other families.Important areas(IAs)of meso-fish are mainly distributed near the Antarctic Peninsula and East Antarctica.Negotiated protected area cover 23% of IAs at present and 38%of IAs in the future(RCP8.5,long-term future).Many IAs of meso-fish still need to be included in protected areas,such as the Prydz Bay and the seas around the Antarctic Peninsula.Our results provide a framework for evaluating protected areas incorporating climate change adaptation strategies for protected areas management. | Shuhao Liu Yang Liu Katharina Teschke Mark AHindell Rachel Downey Briannyn Woods Bin Kang Shuyang Ma Chi Zhang Jianchao Li Zhenjiang Ye Peng Sun Jianfeng He Yongjun Tian | 2024 | Marine Life Science & Technology2024，6，1： | 0 |
| 13 | Research Center of 3D Bioprinting in Shanghai Ninth People’s Hospital显示文摘The center was first established in 2013 and affiliated to the Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine.In 2015,the center was entitled as 3D Bioprinting Clinical Transformation Collaborative Innovation Center.In 2016,the center was renamed as Medical 3D Printing Innovation Research Center of Shanghai Jiao Tong University School of Medicine.Although the center was established in 2013,the clinical application of 3D printing in Shanghai Ninth People’s Hospital can be traced back to the 1990s.As early as 30 years ago,Prof.Kerong Dai had tried to rebuild patients pelvic by manually cutting the foam boards and layering them together based on the theory of 3D printing for preoperative planning model of abnormal skeleton structures and development of personalized prosthesis.That was the first application of 3D printing in the area of medicine in China.Now,The Research Center of 3D Bioprinting in Shanghai Ninth People’s Hospital comprised of subcenters including the outpatient department of 3D printing,medical 3D printing innovation research center and Shanghai key laboratory of orthopedic implant. | Tao Li Xiaojun Zhou Changxu Deng Xiaoxiao Yang Han Yang Zhenjiang Ma Lan Cao Zezheng Yang Lei Qiang Jinwu Wang Kerong Dai | 2019 | Bio-Design and Manufacturing2019，2，3： | 0 |
| 14 | MXRA7 is involved in megakaryocyte differentiation and platelet production显示文摘Matrix remodeling is a critical process in hematopoiesis.The biology of MXRA7,as a matrix remodeling associated gene,has still not been reported in hematopoietic process.Public databases showed that MXRA7 expressed in hematopoietic stem cells,suggesting that it may be involved in hematopoiesis.We found that the amounts of megakaryocytes were lower in bone marrow and spleen from Mxra7−/−mice compared with that from wild-type mice.Knock-out of MXRA7 also reduced the amount of platelet in peripheral blood and affected the function of platelets.Knock-out of MXRA7 inhibited hematopoietic stem/progenitor cells differentiate to megakaryocytes possibly through down-regulating the expression of GATA-1 and FOG-1.Moreover,knockdown of MXRA7 in MEG-01 cells could inhibit the cell proliferation and cell apoptosis.Knockdown of MXRA7 inhibited the differentiation of MEG-01 cells and proplatelet formation through suppressing the ERK/MAPK signaling pathway and the expression ofβ-tubulin.In conclusion,the current study demonstrated the potential significance of MXRA7 in megakaryocyte differentiation and platelet production.The novel findings proposed a new target for the treatment of platelet-related diseases,and much more investigations are guaranteed to dissect the mechanisms of MXRA7 in megakaryocyte differentiation and platelet production. | Zhenjiang Sun Benfang Wang Ying Shen Kunpeng Ma Ting Wang Yiqiang Wang Dandan Lin | 2023 | Blood Science2023，5，3： | 0 |
| 15 | 3D-Printed Scaffolds Promote Angiogenesis by Recruiting Antigen-Specific T Cells显示文摘The immune response after implantation is a primary determinant of the tissue-repair effects of threedimensional(3D)-printed scaffolds.Thus,scaffolds that can subtly regulate immune responses may display extraordinary functions.Inspired by the angiogenesis promotion effect of humoral immune response,we covalently combined mesoporous silica micro rod(MSR)/polyethyleneimine(PEI)/ovalbumin(OVA)self-assembled vaccines with 3D-printed calcium phosphate cement(CPC)scaffolds for local antigen-specific immune response activation.With the response activated,antigen-specific CD4+T helper2(Th2)cells can be recruited to promote early angiogenesis.The silicon(Si)ions from MSRs can accelerate osteogenesis,with an adequate blood supply being provided.At room temperature,scaffolds with uniformly interconnected macropores were printed using a self-setting CPC-based printing paste,which promoted the uniform dispersion and structural preservation of functional polysaccharides oxidized hyaluronic acid(OHA)inside.Sustained release of OVA was achieved with MSR/PEI covalently attached to scaffolds rich in aldehyde groups as the vaccine carrier.The vaccine-loaded scaffolds effectively recruited and activated dendritic cells(DCs)for antigen presentation and promoted the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)in vitro.When embedded subcutaneously in vivo,the vaccine-loaded scaffolds increased the proportion of Th2 cells in the spleen and locally recruited antigenspecific T cells to promote angiogenesis in and around the scaffold.Furthermore,the result in a rat skull defect-repair model indicated that the antigen-specific vaccine-loaded scaffolds promoted the regeneration of vascularized bone.This method may provide a novel concept for patient-specific implant design for angiogenesis promotion. | Cuidi Li Zhenjiang Ma Wentao Li Tianyang Jie Liping Zhong Hongfang Chen Wenhao Wang Jinwu Wang Wenguo Cui Yongxiang Zhao | 2022 | Engineering2022，，10： | 0 |
| 16 | Indirubin-30 -monoxime-loaded PLGA-PEG nanoparticles for potential Alzheimer's disease treatment显示文摘Alzheimer's disease(AD)is an irreversible neurodegenerative disorder,which is pathologically characterized by the deposits of β-amyloid(Aβ),and plays an important role in neuronal death.Indirubin-30-monoxime(I3M)showed neuroprotective effects against Aβ-induced neuronal apoptosis.However,the use of I3M in AD treatment is limited due to its low bioavailability.Herein,PLGA-PEG nanoparticles were synthesized for I3M loading.I3M could release sustainedly sustain release from the I3M-loaded PLGA-PEG nanoparticles(PLGA-PEG-I3M NPs)without obvious burst release.What's more,the PLGA-PEG-I3M NPs could significantly promote the uptake of I3M by PC12 cells through nanoparticle-mediated transport,and improve the efficacy of I3M on the inhibition of Aβfibrillization and oligomerization as well as the neuroprotective activity of I3M on Aβoligomers-induced neuronal death.Thus,the PLGA-PEG-I3M NPs may be a promising platform for AD therapy. | Lingli Jin Qiyao Wang Mengxiang Yang Jiaying Zhang Hongze Liang Hui Tan Zhenjiang Liang Xiaopeng Ma Junying Liu Haiyan Li Xiaodong Cai Wei Cui Lingling Zhao | 2022 | Medicine in Novel Technology and Devices2022，，3： | 0 |
| 17 | Zwitterionic ring-opening polymerization of macrocyclic ethyleneoxy-substituted carbonate:Access to cyclic PEG-like polycarbonate显示文摘The innovation in polymer design to rival conventional polyethylene glycol(PEG)is an important approach to achieving a more sustainable society.Here,cyclic PEG-like polycarbonates having high molecular weight(4.4–49.5 kg/mol)were enabled through zwitterionic ring-opening polymerization(ZROP)of macrocyclic carbonates(MCs)mediated by N-heterocyclic carbene(NHC).The thermodynamic behavior of polymerization depends on the ring size of monomers.During this process,the ZROP of 11-membered MC was driven by the change of enthalpy(ΔH_(p))which differed from the ZROP of 14-membered MC driven by the entropic change(ΔS_(p)).Cyclic polycarbonates depicted improved thermostability(T_(d5%)≥204℃)and higher glass transition temperatures(T_(g)>–40℃)in comparison to their linear analogues(T_(d5%)≤185℃,T_(g)~–50℃).In addition,the mechanism of ZROP of MC was addressed through computational study.A distinct mechanism of polymerization distinguishable from the well-known NHC-mediated ZROP of cyclic esters was revealed,where the zwitterion from nucleophilic addition to MC,i.e.tetrahedral intermediate,cannot be ring-opened probably due to the delocalization of negative charge on the carbonate group,but serves as an active center for the polymerization.In comparison to PEG,the attained polymer demonstrated comparable hydrophilic and biocompatible properties,as revealed by the results of contact angle and in vitro cytotoxicity studies,suggesting that cyclic polycarbonate hold the promise as the alternative of PEG. | Jin Huang Jinwen Li Rui Yan Yuanyuan Qu Fengzhen Guo Lei Shen Can-liang Ma Jie Sun Zhenjiang Li Kai Guo | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 18 | Graphdiyne scaffold anchored highly dispersed ruthenium nanoparticles as an efficient cathode catalyst for rechargeable Li-CO_(2) battery显示文摘Lithium (Li)-CO_(2) battery is rising as an attractive energy-storage system with the competence of CO_(2) conversion/fixation. However, its practical development is seriously hindered by the high overpotential. Herein, a rational design on a highly catalytic Li-CO_(2) battery electrode built by graphdiyne powder as a multi-functional laminar scaffold with anchored highly dispersed Ru nanoparticles is explored. The strong interaction between the abundant acetylenic bond sites of graphdiyne scaffold and Ru nanoparticles can effectively promote the electrochemical progress and reduce the voltage polarization. The unique channels architecture of the cathodic catalyst with enough space not only accelerates CO_(2) diffusion and electrons/Li+ transport, but also allows a large amount of accommodation for discharged product (Li2CO3) to assure an advanced capacity. The corresponding Li-CO_(2) battery displays an advanced discharged capacity of 15,030 mAh/g at 500 mA/g, great capacity retention of 8873 mAh/g at 2 A/g, high coulombic efficiency of 97.6% at 500 mA/g and superior life span for 120 cycles with voltage gap of 1.67 V under a restricted capacity of 1000 mAh/g at 500 mA/g. Ex/in-situ studies prove that synergy between Ru nanoparticles and acetylene bonds of GDY can boost the round-trip CO_(2)RR and CO_(2)ER kinetics. | Yiru Ma Huiqi Qu Wenna Wang Ziyang Guo Yueqin Yu Feng Liu Bin Yu Minge Tian Zhenjiang Li Bin Li Lei Wang | 2024 | Chinese Chemical Letters2024，35，1： | 0 |
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