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| 1 | Isolation of recombinant field strains of Marek's disease virus integrated with reticuloendotheliosis virus genome fragments显示文摘Two Marek's disease virus (MDV) field strains were isolated from chickens with tumors independently from Guangdong and Guangxi provinces, and it was confirmed that there were no co-infections with reticuloendotheliosis viruses (REV) in chicken embryo fibroblast cells (CEF) in indirect fluorescence antibody test (IFA) with REV-specific monoclonal antibodies. By dot blot hybridization and PCR of genomic DNA of MDV-infected CEF, it was indicated that LTR fragments of REV genome were integrated into genome of these two MDV field strains. To amplify and clone the integrated REV LTR with MDV sequence at the junction, 4 primers from REV LTR and 7 primers from MDV genome fragment with REV LTR insertion hot points were synthesized and 28 (4x7) pairs of primers (one from REV and another from MDV for each pair) were used in PCR while using the genomic DNA of both strains as the templates. The sequence data demonstrated that both recombinant field strains contained the same REV LTR inserted into MDV at the identical sites in US fragment of the genomes. From the above, it was speculated that both recombinant field MDVs were originated from a same recombinant virus and spread among chicken flocks in two provinces. | ZHANG Zhi CUI Zhizhong | 2005 | Science China(Life Sciences)2005，48，1： | 24 |
| 2 | WAVELET METHOD FOR CALCULATING THE DEFECT STATES OF TWO-DIMENSIONAL PHONONIC CRYSTALS显示文摘Based on the variational theory,a wavelet-based numerical method is developed to calculate the defect states of acoustic waves in two-dimensional phononic crystals with point and line defects.The supercell technique is applied.By expanding the displacement field and the material constants (mass density and elastic stiffness) in periodic wavelets,the explicit formula- tions of an eigenvalue problem for the plane harmonic bulk waves in such a phononic structure are derived.The point and line defect states in solid-liquid and solid-solid systems are calcu- lated.Comparisons of the present results with those measured experimentally or those from the plane wave expansion method show that the present method can yield accurate results with faster convergence and less computing time. | Zhizhong Yah Yuesheng Wang Chuanzeng Zhang | 2008 | Acta Mechanica Solida Sinica2008，21，2： | 16 |
| 3 | Prognostic value of preoperative prognostic nutritional index and its associations with systemic inflammatory response markers in patients with stage Ⅲ colon cancer显示文摘Background: The prognostic nutritional index(PNI) has been widely applied for predicting survival outcomes of patients with various malignant tumors. Although a low PNI predicts poor prognosis in patients with colorectal cancer after tumor resection, the prognostic value remains unknown in patients with stage Ⅲ colon cancer undergoing curative tumor resection followed by adjuvant chemotherapy. This study aimed to investigate the prognostic value of PNI in patients with stage III colon cancer.Methods: Medical records of 274 consecutive patients with stage Ⅲ colon cancer undergoing curative tumor resection followed by adjuvant chemotherapy with oxaliplatin and capecitabine between December 2007 and December2013 were reviewed. The optimal PNI cutoff value was determined using receiver operating characteristic(ROC) curve analysis. The associations of PNI with systemic inflammatory response markers, including lymphocyte-to-monocyte ratio(LMR), neutrophil-to-lymphocyte ratio(NLR), platelet-to-lymphocyte ratio(PLR), and C-reactive protein(CRP)level, and clinicopathologic characteristics were assessed using the Chi square or Fisher's exact test. Correlation analysis was performed using Spearman's correlation coefficient. Disease-free survival(DFS) and overall survival(OS)stratified by PNI were analyzed using Kaplan-Meier method and log-rank test, and prognostic factors were identified by Cox regression analyses.Results: The preoperative PNI was positively correlated with LMR(r= 0.483, P < 0.001) and negatively correlated with NLR(r =-0.441, P < 0.001), PLR(r =-0.607, P < 0.001), and CRP level(r =-0.333, P < 0.001). A low PNI(≤49.22)was significantly associated with short OS and DFS in patients with stage ⅢC colon cancer but not in patients with stage ⅢA/ⅢB colon cancer.In addition, patients with a low PNI achieved a longer OS and DFS after being treated with6-8 cycles of adjuvant chemotherapy than did those with < 6 cycles. Multivariate analyses revealed that PNI was independently associated with DFS(hazard ratios 2.001; 95% confidence interval 1.157-3.462; P = 0.013).Conclusion: The present study identified preoperative PNI as a valuable predictor for survival outcomes in patients with stage Ⅲ colon cancer receiving curative tumor resection followed by adjuvant chemotherapy. | Jianhong Peng Rongxin Zhang Yixin Zhao Xiaojun Wu Gong Chen Desen Wan Zhenhai Lu Zhizhong Pan | 2017 | Chinese Journal of Cancer2017，36，11： | 11 |
| 4 | An efficient rendering method for large vector data on large terrain models显示文摘This paper presents an efficient way to render large-scale vector maps on level-of-detail(LOD) digital elevation models(DEMs).By using the frame buffer and Voronoi diagram,we achieve a rapid simplification of large-scale vector maps while keeping their original topological relationships.With the simplified maps,we establish level-of-detail vector map models.In the detailed level,we use the stencil shadow volume approach to render the map accurately,and in the coarse level we use substitute techniques to render the map approximately yet more quickly.The method proposed in this paper is universally applicable to rendering vector maps on various LOD terrain models,and the overlaying performance is independent of the complexity of underlying terrain surfaces.By use of the view frustum culling and sub-region index techniques,the impact of complexity of vector maps is reduced,and the rendering time is restricted to a certain scope.Thereby,we can achieve seamless and rapid rendering of large-scale vector maps on LOD terrain surfaces. | YANG Ling ZHANG LiQiang KANG ZhiZhong XIAO ZhiQiang PENG JunHuan ZHANG XingMing LIU Liu | 2010 | Science China(Information Sciences)2010，53，6： | 11 |
| 5 | Study of Furoxan Derivatives for Energetic Applications显示文摘二新奇精力充沛的混合物, 3,4 二度(1,2,4-triazole-3-yl ) furoxan (BTAF ) 和 3,4 二度(1-nitro-1,2,4-triazole-3-yl ) furoxan (BNTAF ) ,被准备,他们的结构被红外, 1H NMR, 13C NMR, MS 技术和元素的分析描绘。BTAF 和 BNTAF 的性质被估计。BTAF 的预言的性能数据如下:密度(测量) 是 1.75 g/cm3,氮内容 50.9% ,形成 +419.7 kJ/mol 的爆炸速度 7277 m/s,爆炸压力 20.1 GPa 和热含量。BNTAF 的预言的表演数据如下:密度是 1.84 g/cm3,氮内容 45.2% ,形成 +841.5 kJ/mol,爆炸速度 8490 m/s 和爆炸压力的热含量 32.4 GPa。BTAF 和 BNTAF 的主要 themal 性质被 DSC 和 TG 技术分析,结果证明 BTAF 在 188.8 攠桸扩瑩摥栠杩 ? 敳敬瑣癩瑩 ? 湡 ? 湡楴楤瑳牵慢据 ? 潦 ? 敆 ? 愠潭杮攠癮物湯敭瑮污祬爠汥癥湡 ? 敭慴? 潩獮椠 ? 煡敵畯 ? 与伴随物分解融化敭楤? 吠敨洠瑥潨 ? 景 ? 扯猧瀠潬 ? 湩楤慣整 ? 桴 ? 潦浲瑡潩 ? 景ㄠ呼挠浯汰硥戠瑥敷湥 ? 愠摮吗?? | Yanan Li Zhizhong Zhang Zhongxue Ge BozhouWang Weipeng Lai Yifen Luo | 2013 | Chinese Journal of Chemistry2013，31，4： | 10 |
| 6 | Ⅲ期结肠癌患者术前预后营养指数的预后价值及其与全身炎症反应标志物的相关性显示文摘背景与目的预后营养指数(prognostic nutritional index,PNI)已广泛应用于预测各种恶性肿瘤患者的生存结局。虽然低PNI预测肿瘤切除后的结直肠癌患者的预后不良,但对接受根治性肿瘤切除术后辅助化疗的Ⅲ期结肠癌患者的预后价值仍未知。本研究旨在探讨PNI对Ⅲ期结肠癌患者的预后价值。方法本文回顾了2007年12月至2013年12月期间,274例接受根治性肿瘤切除术后接受奥沙利铂和卡培他滨辅助化疗的Ⅲ期结肠癌的连续患者的医疗记录。采用受试者工作特征(receiver operating characteristic,ROC)曲线分析确定最佳PNI临界值。采用卡方检验或Fisher’s精确检验评估PNI与全身炎症反应标志物[包括淋巴细胞与单核细胞比值(lymphocyte-to-monocyte ratio,LMR)、中性粒细胞与淋巴细胞比值(neutrophil-to-lymphocyte ratio,NLR)、血小板与淋巴细胞比值(platelet-to-lymphocyte ratio,PLR)、C反应蛋白(C-reactive protein,CRP)水平]和临床病理特征的相关性。采用Spearman’s相关系数进行相关分析。采用Kaplan-Meier法和log-rank检验分析PNI分层的无病生存期(disease-free survival,DFS)和总生存期(overall survival,OS),并通过Cox回归分析确定预后因子。结果术前PNI与LMR呈正相关(r=0.483,P <0.001),与NLR(r=-0.441,P <0.001)、PLR(r=-0.607,P <0.001)和CRP水平(r=-0.333,P <0.001)呈负相关。在ⅢC期结肠癌患者中,低PNI(≤49.22)与短OS和DFS显著相关,而在ⅢA/ⅢB期结肠癌患者中则不相关。此外,在PNI低的患者中,接受6–8个疗程辅助化疗的患者比接受<6个疗程治疗的患者获得了更长的OS和DFS。多变量分析显示PNI与DFS独立相关(风险比=2.001;95%置信区间:1.157–3.462;P=0.013)。结论术前PNI是接受根治性肿瘤切除术后辅助化疗的Ⅲ期结肠癌患者生存结局的重要预测指标。 | Jianhong Peng Rongxin Zhang Yixin Zhao Xiaojun Wu Gong Chen Desen Wan Zhenhai Lu Zhizhong Pan | 2019 | 癌症2019，38，1： | 10 |
| 7 | ARF2 coordinates with PLETHORAs and PINs to orchestrate ABA-mediated root meristem activity in Arabidopsis显示文摘Multiple hormones, including abscisic acid(ABA) and auxin, regulate cell division and differentiation of Arabidopsis root meristems. AUXIN RESPONSE FACTOR2(ARF2) functions as a negative regulator of ABA responses, as seed germination and primary root growth of arf2 mutants are hypersensitive to ABA. In this study, we found that ABA treatment reduced the expression levels of the PIN-FORMEDs(PIN) auxin efflux carriers, PIN1, PIN3,PIN4, and PIN7, to a greater extent in the root meristems of arf2-101 mutant than in the wild type. Also, arf2-101 pin1 and arf2-101 pin4 double mutants show less ABA-induced inhibition of root meristem activity than the arf2-101 mutants. Furthermore, ARF2 positively mediates the transcripts of transcription factor PLETHORA 1(PLT1)gene but negatively mediates PLT2 at protein level in root meristems. Using a dexamethasone(DEX)-inducible transgenic line, Pro35S:PLT2-GR, we showed that PLT2 greatly promotes cell division and completely inhibits cell differentiation in root meristems of the arf2-101 mutant once PLT2 is induced by DEX, which can be partially reversed by ABA treatment, suggesting that ABA regulates root meristem activity in both ARF2-dependent and independent pathways. Our results uncover a complex regulatory architecture in which ARF2 coordinates with PLTs and PINs to orchestrate ABA-mediated regulation of root meristem activity in Arabidopsis. | Sujittra Promchuea Yujuan Zhu Zhizhong Chen Jing Zhang Zhizhong Gong | 2017 | Journal of Integrative Plant Biology2017，59，1： | 9 |
| 8 | A methodology for 3D modeling and visualization of geological objects显示文摘Geological body structure is the product of the geological evolution in the time dimension, which is presented in 3D configuration in the natural world. However, many geologists still record and process their geological data using the 2D or 1D pattern, which results in the loss of a large quantity of spatial data. One of the reasons is that the current methods have limitations on how to express underground geological objects. To analyze and interpret geological models, we present a layer data model to organize different kinds of geological datasets. The data model implemented the unification expression and storage of geological data and geometric models. In addition, it is a method for visualizing large-scaled geological datasets through building multi-resolution geological models rapidly, which can meet the demand of the operation, analysis, and interpretation of 3D geological objects. It proves that our methodology is competent for 3D modeling and self-adaptive visualization of large geological objects and it is a good way to solve the problem of integration and share of geological spatial data. | ZHANG LiQiang TAN YuMin KANG ZhiZhong RUI XiaoPing ZHAO YuanYuan LIU Liu | 2009 | Science China Earth Sciences2009，52，7： | 9 |
| 9 | Expression of voltage.gated sodium channel Nav1.5 in non.metastatic colon cancer and its associations with estrogen receptor(ER)-βexpression and clinical outcomes显示文摘Background: Voltage-gated sodium channel 1.5(Nav1.5) potentially promotes the migratory and invasive behaviors of colon cancer cells. Hitherto, the prognostic significance of Nav1.5 expression remains undetermined. The present study aimed to explore the associations of Nav1.5 expression with clinical outcomes and estrogen receptor-β(ER-β)expression in non-metastatic colon cancer patients receiving radical resection.Methods: A total of 269 consecutive patients with pathologically confirmed stages Ⅰ-Ⅲ colon cancer who underwent radical resection were selected. Nav1.5 and ER-β expression was detected by using immunohistochemistry(IHC)on tissue microarray constructed from paraffin-embedded specimens. IHC score was determined according to the percentage and intensity of positively stained cells. Statistical analysis was performed with the X-tile method, k coefficient, Chi square test or Fisher's exact test, logistic regression, log-rank test, and Cox proportional hazards models.Results: We found that Nav1.5 was commonly expressed in tumor tissues with higher mean IHC score as compared with matched tumor-adjacent normal tissues(5.1 ± 3.5 vs. 3.5 ± 2.7, P < 0.001).The high expression of Nav1.5 in colon cancer tissues was associated with high preoperative carcinoembryonic antigen level [odds ratio(OR) = 2.980;95% confidential interval(CI)1.163-7.632; P = 0.023] and high ER-β expression(OR = 2.808; 95% CI 1.243-6.343;p = 0.00 3). Log-rank test results showed that high Nav1.5 expression contributed to a low 5-year disease-free survival(DFS) rate in colon cancer patients(77.2% vs. 92.1%, P = 0.048), especially in patients with high ER-β expression tumor(76.2% vs. 91.3%, P = 0.032). Analysis with Cox proportional hazards model demonstrated that high Nav1.5 expression[hazard ratio(HR) = 2.738; 95% CI 1.100-6.819;P = 0.030] and lymph node metastasis(HR = 2.633; 95% CI 1.632-4.248; P < 0.001) were prognostic factors for unfavorable DFS in colon cancer patients.Conclusions: High expression of Nav1.5 was associated with high expression of ER-β and indicated unfavorable oncologic prognosis in patients with non-metastatic colon cancer. | Jianhong Peng Qingjian Ou Xiaojun Wu Rongxin Zhang Qian Zhao Wu Jiang Zhenhai Lu Desen Wan Zhizhong Pan Yujing Fang | 2017 | Chinese Journal of Cancer2017，36，12： | 7 |
| 10 | Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures显示文摘The aim of our study was to assess differences in the expression of genes involved in fruit softening and ethylene biosynthetic pathways under different temperature storage conditions. Different peach cultivars of ‘Xiacui' and ‘Yumyeong', which are stonyhard, ‘Yinhualu', which is softmelting, ‘Hujing Milu', which is hard-melting, and ‘Baby Gold 6', which is non-melting at 80% ripening, were collected as test materials. The results showed that only slight ethylene production was detected after harvesting of ‘Yumyeong' and ‘Xiacui' under either a room temperature(25 °C) or low temperature(4 °C). The fruit firmness of stonyhard cultivars was retained at a high level under room temperature over time, whereas a low temperature induced ‘Yumyeong' fruit to soften. Quantitative real-time PCR results indicated that the PpACS1 gene was highly expressed in soft-melting, hard-melting and non-melting cultivars; however, expression was extremely low in stonyhard peaches. PpACS2 or PpACS3, however,was not detected in all five cultivars. Interestingly, cold treatment significantly decreased firmness along with endo-PG expression obviously upregulated in ‘Yumyeong', but not in ‘Xiacui' peaches. In conclusion, this study revealed that fruit softening of peaches with different flesh textures was closely related to ethylene biosynthesis during the storage period, which was controlled via regulating relevant gene expression levels under different storage temperatures. | YANG Yong MA Ruijuan ZHANG Binbin SONG Zhizhong ZHANG Chunhua GUO Shaolei YU Mingliang | 2016 | Horticultural Plant Journal2016，2，2： | 7 |
| 11 | Thermodynamic Characteristic Study of a High-temperature Flow-rate Control Valve for Fuel Supply of Scramjet Engines显示文摘Thermodynamic characteristics are of great importance for the performance of a high-temperature flow-rate control valve,as high-temperature environment may bring problems,such as blocking of spool and increasing of leakage,to the valve.In this paper,a high-temperature flow-rate control valve,pilot-controlled by a pneumatic servo system is developed to control the fuel supply for scramjet engines.After introducing the construction and working principle,the thermodynamic mathematical models of the valve are built based on the heat transfer methods inside the valve.By using different boundary conditions,different methods of simulations are carried out and compared.The steady-state and transient temperature field distribution inside the valve body are predicted and temperatures at five interested points are measured.By comparing the simulation and experimental results,a reasonable 3D finite element analysis method is suggested to predict the thermodynamic characteristics of the high-temperature flow-rate control valve. | ZENG Wen TONG Zhizhong LI Songjing LI Hongzhou ZHANG Liang | 2012 | Chinese Journal of Aeronautics2012，25，4： | 6 |
| 12 | Intelligent identification of two-dimensional nanostructures by machine-learning optical microscopy显示文摘Two-dimensional (2D)materials and their heterostructures,with wafer-scale synthesis methods and fascinating properties,have attracted significant interest and triggered revolutions in corresponding device applications.However,facile methods to realize accurate,intelligent,and large-area characterizations of these 2D nanostructures are still highly desired.Herein,we report the successful application of machine-learning strategy in the optical identification of 2D nanostructures.The machine-leaming optical identification (MOI)method endows optical microscopy with intelligent insight into the characteristic color information of 2D nanostructures in the optical photograph.The experimental results indicate that the MOI method enables accurate,intelligent,and large-area characterizations of graphene,molybdenum disulfide,and their heterostructures, including identifications of the thickness,existence of impurities,and even stacking order.With the convergence of artificial intelligence and nanoscience,this intelligent identification method can certainly promote fundamental research and wafer-scale device applications of 2D nanostructures. | Xiaoyang Lin Zhizhong Si Wenzhi Fu Jianlei Yang Side Guo Yuan Cao Jin Zhang Xinhe Wang Peng Liu Kaili Jiang Weisheng Zhao | 2018 | Nano Research2018，11，12： | 5 |
| 13 | Biodegradable metals for bone defect repair:A systematic review and meta-analysis based on animal studies显示文摘Biodegradable metals are promising candidates for bone defect repair.With an evidence-based approach,this study investigated and analyzed the performance and degradation properties of biodegradable metals in animal models for bone defect repair to explore their potential clinical translation.Animal studies on bone defect repair with biodegradable metals in comparison with other traditional biomaterials were reviewed.Data was carefully collected after identification of population,intervention,comparison,outcome,and study design(PICOS),and following the inclusion criteria of biodegradable metals in animal studies.30 publications on pure Mg,Mg alloys,pure Zn and Zn alloys were finally included after extraction from a collected database of 2543 publications.A qualitative systematic review and a quantitative meta-analysis were performed.Given the heterogeneity in animal model,anatomical site and critical size defect(CSD),biodegradable metals exhibited mixed effects on bone defect repair and degradation in animal studies in comparison with traditional non-degradable metals,biodegradable polymers,bioceramics,and autogenous bone grafts.The results indicated that there were limitations in the experimental design of the included studies,and quality of the evidence presented by the studies was very low.To enhance clinical translation of biodegradable metals,evidence-based research with data validity is needed.Future studies should adopt standardized experimental protocols in investigating the effects of biodegradable metals on bone defect repair with animal models. | Jiazhen Zhang Yanbiao Jiang Zhizhong Shang Bing Zhao Mingyue Jiao Wenbo Liu Maobo Cheng Bao Zhai Yajuan Guo Bin Liu Xinli Shi Bin Ma | 2021 | Bioactive Materials2021，6，11： | 5 |
| 14 | Web-based visualization of spatial objects in 3DGIS显示文摘Adaptive rendering large and complex spatial data has become an important research issue in a 3DGIS application. In order to transmit the data to the client efficiently,this paper proposes a node-layer data model to manage the 3D scene. Because the large spatial data and limited network bandwidth are the main bottlenecks of web-based 3DGIS,a client/server architecture including progressive transmission methods and multiresolution representations,together with the spatial index,are developed to improve the performance. All this makes the application quite scalable. Experimental results reveal that the application works appropriately. | ZHANG LiQiang GUO ZhiFeng KANG ZhiZhong ZHANG LiXin ZHANG XingMing YANG Ling | 2009 | Science in China(Series F)2009，52，9： | 5 |
| 15 | GaN-based substrates and optoelectronic materials and devices显示文摘In order to solve the problems of GaN heteroepitaxy on sapphire substrate,some techniques were explored.Freestanding GaN substrates have been made by hydride vapor phase epitaxy(HVPE),laser lift-off(LLO),and chemical mechanical polishing techniques.Wafer bending and cracking in the HVPE growth were partly settled by pulsed flow modulation method.High-crystal quality was established for 1.2 mm thick GaN substrate by X-ray diffraction measurement,in which the full width of half maximum values were 72,110 arcsec for(102),(002)peaks.A novel micro-size patterned sapphire substrate(PSS)and a nano PSS were also fabricated.High-power vertical structure light emitting diodes(VSLEDs)have been developed by Au–Sn eutectic wafer bonding,homemade micro-area LLO,and light extraction structure preparation.The high-injection-level active region with low temperature GaN sandwiched layers was used for lowefficiency droop.The light output power of VSLED was achieved as 400 mW driven at 350 mA,and the dominant wavelength is about 460 nm.The structures and properties of strain modulated superlattices(SLs)and quantum wells as well as advanced simulation of carriers transport across the electron blocking layer were investigated in laser diodes.The hole concentration was achieved as high as1.6 9 1018cm-3in AlGaN/GaN SLs:Mg by inserting an AlN layer.High-quality AlGaN epilayers and structures were grown by MOCVD.Some device structures of UV LEDs and detectors were demonstrated.The emission wavelength of 262 nm UV LED has been successfully fabricated.At last,high-quality InN and InGaN materials for solar cell were grown by boundary-temperature-controlled epitaxy and growth-temperature-controlled epitaxy.Hall-effect measurement showed a recorded electron mobility of 3,280 cm2/(V s)and a residual electron concentration of 1.47 9 1017cm-3at 300 K. | Guoyi Zhang Bo Shen Zhizhong Chen Xiaodong Hu Zhixin Qin Xinqiang Wang Jiejun Wu Tongjun Yu Xiangning Kang Xingxing Fu Wei Yang Zhijian Yang Zhizhao Gan | 2014 | Chinese Science Bulletin2014，59，12： | 4 |
| 16 | Comparison of clinical outcomes of Chinese men and women after coronary stenting for coronary artery disease:a multi-center retrospective analysis of 4,334 patients显示文摘The outcome differences between Chinese male and female patients within one-year follow-up after percutaneous coronary intervention(PCI) with stent remain unclear.The present study was aimed to compare clinical outcomes in such two populations.From May 1999 to December 2009,4,334 patients with acute myocardial infarction(MI),unstable angina,stable angina,or silent ischemia,who underwent PCI,were registered at our centers.Among these,3,089 were men and 1,245 were women.We compared these groups with respect to the primary outcomes of MI and secondary outcomes including a composite of major adverse cardiac events(MACE) including cardiac death,MI,target lesion revascularization,target vessel revascularization(TVR),stent thrombosis(ST),definite ST and probable ST at one-year follow-up.Chinese male patients had a higher MACE rate(13%vs.10.7%,P =0.039),mainly led by TVR(9.09%vs.6.98%,P=0.024) at one year,which was significantly different than female patients.Chinese male and female patients showed a significant difference on MACEs.However,there was no significant difference with respect to MI between these groups. | Rajiv Shrestha Jing Xu Dujiang Xie Zhizhong Liu Tian Xu Fei Ye Shiqing Din Xuesong Qian Song Yang Yueqiang Liu Feng Li Aiping Zhang Shaoliang Chen | 2014 | The Journal of Biomedical Research2014，28，5： | 3 |
| 17 | PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness显示文摘PHYTOCHROME-INTERACTING FACTORS(PIFs)are a group of basic helix-loop-helix transcription factors that can physically interact with photoreceptors,including phytochromes and cryptochromes.It was previously demonstrated that PIFs accumulated in darkness and repressed seedling photomorphogenesis,and that PIFs linked different photosensory and hormonal pathways to control plant growth and development.In this study,we show that PIFs positively regulate the ABA signaling pathway during the seedling stage specifically in darkness.We found that PIFs positively regulate ABI5 transcript and protein levels in darkness in response to exogenous ABA treatment by binding directly to the G-box motifs in the ABI5 promoter.Consistently,PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness,and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark.Moreover,we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9,and that this interaction is not regulated by ABA.Further analyses showed that PYL8 and PYL9 promote PIF4 protein accumulation in the dark and enhance PIF4 binding to the ABI5 promoter,but negatively regulate PIF4-mediated ABI5 activation.Taken together,our data demonstrate that PIFs interact with ABA receptors to orchestrate ABA signaling in darkness by controlling ABI5 expression,providing new insights into the pivotal roles of PIFs as signal integrators in regulating plant growth and development. | Lijuan Qi Shan Liu Cong Li Jingying Fu Yanjun Jing Jinkui Cheng Hong Li Dun Zhang Xiaoji Wang Xiaojing Dong Run Han Bosheng Li Yu Zhang Zhen Li William Terzaghi Chun-Peng Song Rongcheng Lin Zhizhong Gong Jigang Li | 2020 | Molecular Plant2020，13，3： | 3 |
| 18 | The direct targets of CBFs:In cold stress response and beyond显示文摘Cold acclimation in Arabidopsis thaliana triggers a significant transcriptional reprogramming altering the expression patterns of thousands of cold-responsive(COR) genes. Essential to this process is the C-repeat binding factor(CBF)-dependent pathway, involving the activity of AP2/ERF(APETALA2/ethylene-responsive factor)-type CBF transcription factors required for plant cold acclimation. In this study, we performed chromatin immunoprecipitation assays followed by deep sequencing(ChIP-seq) to determine the genomewide binding sites of the CBF transcription factors. Cold-induced CBF proteins specifically bind to the conserved C-repeat(CRT)/dehydrationresponsive elements(CRT/DRE;G/ACCGAC) of their target genes. A Gene Ontology enrichment analysis showed that 1,012 genes are targeted by all three CBFs. Combined with a transcriptional analysis of the cbf1,2,3 triple mutant, we define 146 CBF regulons as direct CBF targets. In addition, the CBF-target genes are significantly enriched in functions associated with hormone, light,and circadian rhythm signaling, suggesting that the CBFs act as key integrators of endogenous and external environmental cues. Our findings not only define the genome-wide binding patterns of the CBFs during the early cold response, but also provide insights into the role of the CBFs in regulating multiple biological processes of plants. | Yue Song Xiaoyan Zhang Minze Li Hao Yang Diyi Fu Jian Lv Yanglin Ding Zhizhong Gong Yiting Shi Shuhua Yang | 2021 | Journal of Integrative Plant Biology2021，63，11： | 3 |
| 19 | Application study of the awning measure to obstruct solar radiation in permafrost regions on the Qinghai-Tibet Plateau显示文摘With globe warming,road safety will change dramatically,especially within the Qinghai-Tibet Plateau permafrost regions.Because of higher elevation and better atmospheric transparency,the Qinghai-Tibet Plateau has stronger radiation than other regions,which can change the daily variation of ground surface temperature on the Plateau.The awning measure(shading board) is one of the actively protected permafrost measures,which was adopted along the Qinghai-Tibet railway and highway and the Qing-Kang Highway in China.Field test results show that embankment surface month mean net radiation is 60-130 W/m2,but the value is below 20 W/m2 under the shading board,and the reducing level of natural net radiation is 80%-90%.The shading board reduced the heat flow entering into the embankment by 80%-90% or more,with heat entering into the soil on the common embankment,but emitting from the embankment under the shading board.At the same time,ground surface temperature under the shading board is 6-8 ℃ lower than the exposed embankment.Test results show that the shading board measure can rapidly and effectively reduce net radiation and heat flow into the embankment,decrease embankment surface and interior temperature,effectively delay increase rate of soil temperature under globe warming,ensure stability and safety of the embankment,and guarantee unblocked road projects in cold and permafrost regions. | WenJie Feng ZhiZhong Sun Zhi Wen GuoYu Li Ze Zhang WenBing Yu | 2012 | Research in Cold and Arid Regions2012，4，2： | 3 |
| 20 | HIF-1-modified BMSCs improve migration and reduce neuronal apoptosis after stroke in rats显示文摘Bone marrow-derived mesenchymal stem cells(BMSCs)have been demonstrated ameliorating neurologic deficits after stroke.Hypoxia-inducible factor-1(HIF-1),the key regulator of cellular responses to low oxygen concentration,can activate multiple genes involving in crucial aspects for neurologic recovery.In this study,we present that rat BMSCs overexpression of HIF-1showed higher expression of HIF-1 target genes in HIF-1-BMSCs,including CXCR4,EPO,and VEGF.BMSCs-mHIF-1 also exhibited an enhanced mobility towards the ischemic area within rat brain.Neural cell apoptosis in ischemic brain shown less severe in rats transplanted with HIF-1-BMSCs.Furthermore,the number of cells expressing neural progenitor markers PAX6 and DCX were increased in BMSCs-mHIF-1-transplanted rats.These results suggest that HIF-1 in BMSCs reduces neuronal apoptosis and promotes neurogenesis after stroke in rats. | YE ZhiZhong YE WeiBiao DENG YuBin WANG Jue ZHOU GuangQian ZHANG XiuQuan | 2013 | Chinese Science Bulletin2013，58，28： | 3 |
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