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1Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryza eichingeri显示文摘Wild rice species is an important source of useful genes for cultivated rice improvement Some accessions of Oryza eichingeri (In = 24, CC) from Africa confer strong resistance to brown planthopper (BPH), whitebacked planthopper (WBPH) and bacterial blight (KB). In the present study, restriction fragments length polymorphism (RFLP) and simple sequence repeats (SSR) analysis were performed on disomic backcross plants between Oryza saliva (2n =24, AA) and O. eichingeri in order to identify the presence of O, eichingeri segments and further to localize BPH-resistant gene. In the introgression lines, 1-6 O. eichingeri segments were detected on rice chromosomes 1, 2, 6, or/and 10. The dominant BPH resistant gene, tentatively named Bphl3(t), was mapped to chromosome 2, being 6.1 and 5.5 cM away from two microsatellite markers RM240 and RM250, respectively. The transfer and localization of this gene from O. eichingeri will contribute to the improvement of BPH resistance in cultivated rice.LIU Guoqing YAN Huihuang FU Qiang QIAN Qian ZHANG Zhitao ZHAI Wenxue ZHU Lihuang 2001Chinese Science Bulletin2001,46,17:24
2Sequencing of Cultivated Peanut, Arachis hypogaea, Yields Insights into Genome Evolution and Oil Improvement显示文摘Cultivated peanut (Arachis hypogaea) is an allotetraploid crop planted in Asia, Africa, and America for edible oil and protein. To explore the origins and consequences of tetraploidy, we sequenced the allotetraploid A. hypogaea genome and compared it with the related diploid Arachis duranensis and Arachis ipaensis genomes. We annotated 39 888 A-subgenome genes and 41 526 B-subgenome genes in allotetraploid peanut. The A. hypogaea subgenomes have evolved asymmetrically, with the B subgenome resembling the ancestral state and the A subgenome undergoing more gene disruption, loss, conversion, and transposable element proliferation, and having reduced gene expression during seed development despite lacking genome-wide expression dominance. Genomic and transcriptomic analyses identified more than 2 500 oil metabolism-related genes and revealed that most of them show altered expression early in seed development while their expression ceases during desiccation, presenting a comprehensive map of peanut lipid biosynthesis. The availability of these genomic resources will facilitate a better understanding of the complex genome architecture, agronomically and economically important genes, and genetic improvement of peanut.Xiaoping Chen Qing Lu Hao Liu Jianan Zhang Yanbin Hong Haofa Lan Haifen Li Jinpeng Wang Haiyan Liu Shaoxiong Li Manish K.Pandey Zhikang Zhang Guiyuan Zhou Jigao Yu Guoqiang Zhang Jiaqing Yuan Xingyu Li Shijie Wen Fanbo Meng Shanlin Yu Xiyin Wang Kadambot H.M.Siddique Zhong-Jian Liu Andrew H.Paterson Rajeev K.Varshney Xuanqiang Liang 2019Molecular Plant2019,12,7:23
3Arrangement of High-standard Basic Farmland Construction Based on Village-region Cultivated Land Quality Uniformity显示文摘As an important constitute of land consolidation, high-standard basic farmland construction is an important means to protect the quantity, quality and ecological environment of cultivated land. Its target not only lies in the increase of cultivated land quantity, but also the improvement of cultivated land quality, agricultural production conditions and ecosystem environments. In the present study, the quality evaluation method and construction arrangement of cultivated land were explored to facilitate the process of decision-making and implementation for high-standard basic farmland construction(HSBFC) with administrative village as the unit. Taking the land comprehensive improvement project area in Quzhou County, Handan City, Hebei Province as a case study, the whole process of the study comprised of three steps: 1) establishment of the evaluation model of cultivated land quality uniformity based on regional optimum cultivated land quality, and construction of the uniformity evaluation index system from the aspects of soil fertility quality, engineering quality, spatial quality and eco-environment quality, according to the new concept of cultivated land quality; 2) calculation of cultivated land quality uniformity by grading indicators, assigning scores and weighting sums, exploring the local homogenization characteristics of regional cultivated land quality through spatial autocorrelation analysis, and analyzing the constraints and transformative potential of barrier factors; 3) arrangement of HSBFC according to the principle of concentration, continuity and priority to the easy operation. The results revealed that the value of farmland quality uniformity for the administrative villages in the study area was between 7.76 and 21.96, and there was a difference between various administrative villages. The regional spatial autocorrelation patterns included High-High(HH), Low-Low(LL), High-Low(HL) and Low-High(LH). These indicate that regional cultivated land quality has local homogenization characteristics. The most restrictive factors in the study area were the medium and low transformation difficulty indexes, including soil organic matter content, farmland shelterbelt network density, field regularity and scale of the field. In addition, there were also high transformation difficulty indicators in some areas, such as sectional configuration. The project area was divided into four partitions: major construction area, secondary construction area, general construction area, and conditional construction area. The cultivated land area of each subarea was 1538.85 ha, 1224.27 ha, 555.93 ha, and 1666.63 ha, respectively. This comprised of 30.87%, 24.56%, 11.15% and 33.42% of the total project area, respectively. The evaluation model and index system could satisfy the evaluation of farmland quality and diagnosis of obstacle factors to facilitate the subsequent construction decision. The present study provides reference for the practice of regional HSBFC, and a new feasible idea and method for related studies.SONG Wen WU Kening ZHAO Huafu ZHAO Rui LI Ting 2019Chinese Geographical Science2019,29,2:12
4Coupling Rice with Fish for Sustainable Yields and Soil Fertility in China显示文摘About 30 million hectares of rice are cultivated annually in China(FAOSTAT,2019).Because most rice fields are wellirrigated and suitable for fish living(‘fish’here refers to various aquatic animals like carps,prawns,crabs,soft shell turtles,etc.)(Fernando,1993),many efforts have been made to determine how well rice farming and aquaculture can be coupled as a rice-fish system(RFS),of which the same land and water produces both rice and aquatic products.With the technological guidance of RFSs and political supports from the Ministry of Agriculture and Rural Affairs of China since 2010(Supplemental Table 1),multiple types of large-scale,high-efficiency and technologically intensive RFSs are now being demonstrated in the main rice-growing areas of China(National Fisheries Technology Extension Centre,2019),and in total about 1.2 million hectares of rice fields are under RFSs.GUO Liang HU Liangliang ZHAO Lufeng SHI Xiaoyu JI Zijun DING Lilian REN Weizheng ZHANG Jian TANG Jianjun CHEN Xin 2020Rice science2020,27,3:11
5Farming and forestry land use changes in China and their driving forces from 1900 to 1980显示文摘A variety of agricultural and forestry land use materials, especially those in the first half of 20th century, were collected. According to land use change in this period, the whole country is demarcated into seven regions, Northeast China, North China, Northwest and Loess Plateau, Southeast and Coastal Region, Southwest China, Qinghai-Tibetan Plateau, Inner Mongolia and Xinjiang, in proper order by using Cluster Analysis. The farming and forestry land use change in these regions were analyzed. The results show that the total cultivated land areas increased in this period, especially in the 1950s and 1960s the cultivated land area increased more significantly, but differed in different regions, and the most rapid increasing rate was 2.63 percent a year. On the other hand, the forestry land area was increasing in most parts of this period, especially after 1949. But in most regions, the decrease of forestry land area at the end of 1970s is also very obvious. It is regarded that the population increase, food production, natural disasters and some related government policies were among the main driving factors for farming and forestry land use change.GE Quansheng DAI Junhu 2005Science China Earth Sciences2005,48,10:10
6Tea plant genomics:achievements,challenges and perspectives显示文摘Tea is among the world’s most widely consumed non-alcoholic beverages and possesses enormous economic,health,and cultural values.It is produced from the cured leaves of tea plants,which are important evergreen crops globally cultivated in over 50 countries.Along with recent innovations and advances in biotechnologies,great progress in tea plant genomics and genetics has been achieved,which has facilitated our understanding of the molecular mechanisms of tea quality and the evolution of the tea plant genome.In this review,we briefly summarize the achievements of the past two decades,which primarily include diverse genome and transcriptome sequencing projects,gene discovery and regulation studies,investigation of the epigenetics and noncoding RNAs,origin and domestication,phylogenetics and germplasm utilization of tea plant as well as newly developed tools/platforms.We also present perspectives and possible challenges for future functional genomic studies that will contribute to the acceleration of breeding programs in tea plants.En-Hua Xia Wei Tong QiongWu ShuWei Jian Zhao Zheng-Zhu Zhang Chao-Ling Wei Xiao-Chun Wan 2020Horticulture Research2020,7,1:9
7Genetic diversity,population structure,and relationships of apricot(Prunus)based on restriction site-associated DNA sequencing显示文摘Single-nucleotide polymorphisms(SNPs)are the most abundant form of genomic polymorphisms and are widely used in population genetics research.Here,high-throughput sequencing was used to examine the genome-level diversity,population structure,and relationships of apricot,which are important for germplasm conservation and molecular breeding.Restriction site-associated DNA sequencing(RAD-seq)was adopted to sequence 168 Prunus spp.accessions distributed in five ecological groups,including 74 accessions of cultivated Prunus armeniaca L.and 94 accessions of wild apricots(P.armeniaca L.and Prunus sibirica L.),which generated 417,961 high-quality SNPs.We used cluster,genetic structure,and principal component analyses to examine the genetic diversities and genetic relationships of the 168 accessions.The Dzhungar-Ili ecological group accessions showed the highest genetic diversity in terms of private allele number,observed heterozygosity,and nucleotide diversity.We speculate that the Central Asian ecological group accessions were domesticated from the Dzhungar-Ili ecological group accessions.The population structure and gene flow of the North China and European ecological group accessions suggested a genetic background of P.sibirica.We argue that the two groups should be considered hybrid swarms connected to P.sibirica by continuous and extensive gene flow.P.armeniaca originated in Northwest China(Ili Valley),subsequently spread throughout Central Asia,and eventually spread to Europe.In addition,selective sweep signatures in P.armeniaca during domestication from wild to cultivated apricots,combined with differentially expressed genes,underlie distinct fruit traits,including sugars,aromas,organic acids,and carotenoids.This study provides substantive and valuable genomic resources that will significantly advance apricot improvement and effective utilization.Wenwen Li Liqiang Liu Yanan Wang Qiuping Zhang Guoquan Fan Shikui Zhang Yatong Wang Kang Liao 2020Horticulture Research2020,7,1:9
8咸宁市耕地时空演变及变化驱动力研究显示文摘耕地的变化是国内外研究的热点与焦点,其数量的多少和质量的好坏关系到一个国家的粮食安全、经济发展和社会进步等各个方面。该文以咸宁市为例,利用耕地数量变化的时间序列模型、动态度模型和垦殖指数变化模型,对耕地的时空演变特征进行定量研究,并应用相关分析法、主成分分析法及回归分析法对耕地演变及驱动力进行分析,得出研究结论,并针对不同的驱动力因子提出相应的对策建议。何国松 张威 段成诚 2010江西农业学报2010,22,9:6
9A chromosome-level reference genome of nonheading Chinese cabbage[Brassica campestris(syn.Brassica rapa)ssp.chinensis]显示文摘Non-heading Chinese cabbage(NHCC)is an important leafy vegetable cultivated worldwide.Here,we report the first high-quality,chromosome-level genome of NHCC001 based on PacBio,Hi-C,and Illumina sequencing data.The assembled NHCC001 genome is 405.33 Mb in size with a contig N50 of 2.83 Mb and a scaffold N50 of 38.13 Mb.Approximately 53%of the assembled genome is composed of repetitive sequences,among which long terminal repeats(LTRs,20.42%of the genome)are the most abundant.Using Hi-C data,97.9%(396.83 Mb)of the sequences were assigned to 10 pseudochromosomes.Genome assessment showed that this B.rapa NHCC001 genome assembly is of better quality than other currently available B.rapa assemblies and that it contains 48,158 protein-coding genes,99.56%of which are annotated in at least one functional database.Comparative genomic analysis confirmed that B.rapa NHCC001 underwent a whole-genome triplication(WGT)event shared with other Brassica species that occurred after the WGD events shared with Arabidopsis.Genes related to ascorbic acid metabolism showed little variation among the three B.rapa subspecies.The numbers of genes involved in glucosinolate biosynthesis and catabolism were higher in NHCC001 than in Chiifu and Z1,due primarily to tandem duplication.The newly assembled genome will provide an important resource for research on B.rapa,especially B.rapa ssp.chinensis.Ying Li Gao-Feng Liu Li-Ming Ma Tong-Kun Liu Chang-Wei Zhang Dong Xiao Hong-Kun Zheng Fei Chen Xi-Lin Hou 2020Horticulture Research2020,7,1:6
10Genetic and phenological variation of tocochromanol(vitamin E)content in wild(Daucus carota L.var.carota)and domesticated carrot(D.carota L.var.sativa)显示文摘Carrot roots(Daucus carota L.var.sativa)produce tocochromanol compounds,collectively known as vitamin E.However,little is known about their types and amounts.Here we determined the range and variation in types and amounts of tocochromanols in a variety of cultivated carrot accessions throughout carrot postharvest storage and reproductive stages and in wild-type roots(Daucus carota L.var.carota).Of eight possible tocochromanol compounds,we detected and quantified α-,and the combined peak for β-and γ-forms of tocopherols and tocotrienols.Significant variation in amounts of tocochromanol compounds was observed across accessions and over time.Large increases in a-tocopherol were noted during both reproductive growth and the postharvest stages.The variation of tocochromanols in carrot root tissue provides useful information for future research seeking to understand the role of these compounds in carrot root tissue or to breed varieties with increased levels of these compounds.Claire H Luby Hiroshi A Maeda Irwin L Goldman 2014Horticulture Research2014,1,1:6
11An eFP browser for visualizing strawberry fruit and flower transcriptomes显示文摘Wild strawberry Fragaria vesca is emerging as an important model system for the cultivated strawberry due to its diploid genome and availability of extensive transcriptome data and a range of molecular genetic tools.Being able to better utilize these tools,especially the transcriptome data,will greatly facilitate research progress in strawberry and other Rosaceae fruit crops.The electronic fluorescent pictograph(eFP)software is a useful and popular tool to display transcriptome data visually,and is widely used in other model organisms including Arabidopsis and mouse.Here we applied eFP to display wild strawberry RNA sequencing(RNA-seq)data from 42 different tissues and stages,including various flower and fruit developmental stages.In addition,we generated eight additional RNA-seq data sets to represent tissues from ripening-stage receptacle fruit from yellow-colored and red-colored wild strawberry varieties.Differential gene expression analysis between these eight data sets provides additional information for understanding fruit-quality traits.Together,this work greatly facilitates the utility of the extensive transcriptome data for investigating strawberry flower and fruit development as well as fruit-quality traits.Charles Hawkins Julie Caruana Jiaming Li Chris Zawora Omar Darwish Jun Wu Nadim Alkharouf Zhongchi Liu 2017Horticulture Research2017,4,1:6
12Developing single nucleotide polymorphism(SNP)markers from transcriptome sequences for identification of longan(Dimocarpus longan)germplasm显示文摘Longan(Dimocarpus longan Lour.)is an important tropical fruit tree crop.Accurate varietal identification is essential for germplasm management and breeding.Using longan transcriptome sequences from public databases,we developed single nucleotide polymorphism(SNP)markers;validated 60 SNPs in 50 longan germplasm accessions,including cultivated varieties and wild germplasm;and designated 25 SNP markers that unambiguously identified all tested longan varieties with high statistical rigor(P<0.0001).Multiple trees from the same clone were verified and off-type trees were identified.Diversity analysis revealed genetic relationships among analyzed accessions.Cultivated varieties differed significantly from wild populations(Fst=0.300;P<0.001),demonstrating untapped genetic diversity for germplasm conservation and utilization.Within cultivated varieties,apparent differences between varieties from China and those from Thailand and Hawaii indicated geographic patterns of genetic differentiation.These SNP markers provide a powerful tool to manage longan genetic resources and breeding,with accurate and efficient genotype identification.Boyi Wang Hua-Wei Tan Wanping Fang Lyndel W Meinhardt Sue Mischke Tracie Matsumoto Dapeng Zhang 2015Horticulture Research2015,2,1:6
13Comparative Study on the Human Driving Force of Cultivated Land and Construction Land Use Change in Hubei Province,China显示文摘Change trend of cultivated land and construction land in Hubei Province is analyzed.The years 1998-2003 are the reduction period of cultivated land;the years 2004-2007 are the increase period of cultivated land;the years 1988-2002 are the slow growth period of construction land;and the years of 2003 and 2007 are the increase period of construction land.Based on related social economic statistical data in 1988-2007,human driving forces on cultivated land and construction land in Hubei Province are analyzed quantitatively and comparatively by using SPSS software and mathematical statistics method.Result shows that driving force factors have negative driving effect on cultivated land,and positive driving effect on construction land.Driving force factors have significant differences of driving effects on cultivated land and construction land.Three principal components affecting the changes of cultivated land and construction land are policy,population and economic development level,and gap between urban and rural living standards.The three principal components have different degrees of sensitivity on the two land use types and the effects direction are reverse.The other factor driving abilities are relatively weak,and these factors have relatively stronger driving force on construction land than that on cultivated land.It is put forward that Hubei Province should implement rational allocation of land resources and scientific and rational use in order to ensure land security and to realize the sustainable development of regional economy and society based on the intensive land use,the planning policy constraints,and the agricultural and industrial production.XU Xin-chuang1,2,CHENG Dong-lai1,LIU Cheng-wu1 1.School of Resources and Environment Science,Xianning College,Xianning 437005,China 2.Institute of Geographical Sciences and Natural Resources Research,CAS,Beijing 100101,China 2010Asian Agricultural Research2010,2,3:5
14Nitrogen and phosphorus balance analysis of cultivated land in Jiangsu plain,China显示文摘Jiangsu plain is located along the downstream of the Yangtze River, the north part of which is also the coast area of the Yellow Sea. The specific location makes the local environmental issues regionally important. This paper studied arable land area, plantation structure, livestock sector data, which shows that livestock sector contributes a big amount of nitrogen and phosphorus, in terms of the demand from the agriculture use in Jiangsu plain. But actually, there is still much mineral fertiliser used in agriculture, which causes the N and P produced from livestock sector being an important source of pollution to the environment. The paper suggests to tighten up manure management, in order to lead to a big reduction in the pollution to both soil and surface water.ZOU Xinqing Pierre Gerber LI Haiyu LIU Qingsong WANG Qian SHEN Jingfen SHI Binwen 2001Chinese Science Bulletin2001,46,S1:5
15Identification and classification of subspecies of Asian cultivated rice and their hybrids显示文摘Having reviewed the major classification systems proposed by various scholars across the world, it is found that \%indica\% and \%japonica\% under \%O.sativa\% L. are two major directions thoroughly differentiated from the Asian cultivated rice, forming the framework of the classification structure. A system with \%indica\% and \%japonica\% as the only two subspecies is therefore reiterated.There are various ways to determine the indica_japonica identity of hybrid rice, but the 'combined morphological trait index'(CMT index) method is more efficient and easier to handle, although the isozyme analysis, molecular marker analysis and grain quality assay methods are also feasible.Xiangkun Wang Renhua Li Chuanqing Sun Zichao Li Hongwei Cai Xinli Sun 1998Chinese Science Bulletin1998,43,22:5
16Spatiotemporal variations of cultivated land use efficiency in the Yangtze River Economic Belt based on carbon emission constraints显示文摘In this study,the carbon emissions(CEs)from cultivated land(CL)were included as an undesirable output in the utilization efficiency of such land.A slack-based model was used to calculate the CL use efficiency(CLUE)for 11 provinces and cities in the Yangtze River Economic Belt(YREB)from 2007 to 2016,and then a kernel density estimation map was drawn to analyze the spatiotemporal variations of CLUE.The Tobit model was also employed to analyze the factors affecting the CLUE.The results show the following.1)In the YREB,the CEs from CL showed a rising and then a slowly decreasing trend.In this paper,we calculate CEs by carbon emission factors and major carbon sources,and the CEs from CL in the YREB totaled 25.2354 million tons in 2007.By 2014,the value had increased gradually to 28.4400 million tons,and by 2016 it had declined to 27.8922 million tons,suggesting that the carbon-emission reduction measures of the government had an impact.2)The CLUE of various provinces and cities in the YREB showed an upward trend in the time dimension,while for the spatial dimension,the kernel density was high in the east and low in the west,and the areas with high kernel density were mainly located in the Yangtze River Delta.3)The per capita gross domestic product,the primary industrial output,and the number of agricultural technicians per 10,000 people had positive effects on the CLUE.The CL area per capita and the electrical power per hectare for agricultural machinery had significant negative impacts on CLUE.In addition,every 1%increase in the number of agricultural technicians increased the CLUE by 0.057%.罗翔 敖心荷 张祚 万庆 刘行健 2020Journal of Geographical Sciences2020,30,4:5
17An AGAMOUS-like factor is associated with the origin of two domesticated varieties in Cymbidium sinense (Orchidaceae)显示文摘Cymbidium has been artificially domesticated for centuries in Asia,which produced numerous cultivated varieties.Flowers with stamenoid tepals or those with multiple tepals have been found in different species of Cymbidium;however,the molecular basis controlling the formation of these phenotypes is still largely unknown.Previous work demonstrated that AGAMOUS/AG lineage MADS genes function in floral meristem determinacy as well as in reproductive organs development in both dicots and monocots,indicating a possible relationship with the origin of two flower varieties in Cymbidium.Here,we characterized and analyzed two AG lineage paralogues,CsAG1 and CsAG2,from Cymbidium sinense,both of which were highly expressed in the gynostemium column of a standard C.sinense.Interestingly,we detected ectopic expression of CsAG1 rather than CsAG2 in all floral organs of a stamenoid-tepal variety and significant down-regulation of CsAG1 in a variety with multiple tepals.Over-expression of CsAG1 in wild type Arabidopsis resulted in petal-to-stamen homeotic conversion,suggesting a conserved C-function of CsAG1 in the development of Cymbidium flower.Altogether,our results supported a hypothesis that disruption of a single AG-like factor would be associated with the formation of two domesticated varieties in C.sinense.Shihao Su Xiaoyu Shao Changfa Zhu Jiayin Xu Yuhuan Tang Da Luo Xia Huang 2018Horticulture Research2018,5,1:4
18A 14 nucleotide deletion mutation in the coding region of the PpBBX24 gene is associated with the red skin of“Zaosu Red”pear(Pyrus pyrifolia White Pear Group):a deletion in the PpBBX24 gene is associated with the red skin of pear显示文摘Red skin is an important quality trait for pear fruits and is determined by the concentration and composition of anthocyanins.The regulatory mechanism underlying anthocyanin accumulation is a popular topic in fruit research.Red mutants are ideal materials for studying the molecular mechanism of color diversity in pear.Although several red pear mutants have been cultivated and are in production,no exact locus containing the responsible genetic mutation has been identified.In this study,by combining the bulked segregant analysis with whole-genome sequencing,we identified a 14 nucleotide deletion mutation in the coding region of the PpBBX24 gene from the red pear mutant“Zaosu Red”.We further verified that the deletion was present only in the red mutant of“Zaosu”and in its red offspring,which was different from that which occurred in other red pear fruits.This deletion results in a coding frame shift such that there is an early termination of the PpBBX24 gene and loss of key NLS and VP domains from PpBBX24.The lost domains may reduce or alter the normal function of PpBBX24.In addition,we found that the transcript levels of the PpMYB10 and PpHY5 genes in red samples were significantly higher than those in green samples,whereas the results for the normal-type PpBBX24 gene were the opposite.We ultimately revealed that the 14 nucleotide deletion mutation in the coding region of the PpBBX24 gene is associated with the red skin of the“Zaosu Red”pear.This finding of somatic mutational events will be helpful for breeding new red pear cultivars and for understanding the regulatory mechanisms involved in pear skin pigmentation.Chunqing Ou Xiaoli Zhang Fei Wang Liyi Zhang Yanjie Zhang Ming Fang Jiahong Wang Jixun Wang Shuling Jiang Zhihong Zhang 2020Horticulture Research2020,7,1:4
19Bt protein expression in the transgenic insect-resistant cotton in China显示文摘According to statistics of International Service for the Acquisition of Agri-biotech Applications(ISAAA),18million farmers in 28 countries planted more than 181million hectares of genetically modified(GM)crops in2014,at an annual growth rate of between 3%–4%,with6.3 million ha more than 2013[1].Among these counties,China ranks the sixth with 3.9 million ha.Globally,27kinds of transgenic crops have been approved.The four main food groups for which GM varieties areGuoqing Sun Dongling Zhang Rui Zhang Yuan Wang Zhigang Meng Tao Zhou Chenzhen Liang Tao Zhu Sandui Guo 2016Science Bulletin2016,61,20:3
20A super pan-genomic landscape of rice显示文摘Pan-genomes from large natural populations can capture genetic diversity and reveal genomic complexity.Using de novo longread assembly,we generated a graph-based super pan-genome of rice consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice.Our pan-genome reveals extensive structural variations(SVs)and gene presence/absence variations.Additionally,our pan-genome enables the accurate identification of nucleotide-binding leucine-rich repeat genes and characterization of their inter-and intraspecific diversity.Moreover,we uncovered grain weight-associated SVs which specify traits by affecting the expression of their nearby genes.We characterized genetic variants associated with submergence tolerance,seed shattering and plant architecture and found independent selection for a common set of genes that drove adaptation and domestication in Asian and African rice.This super pan-genome facilitates pinpointing of lineage-specific haplotypes for trait-associated genes and provides insights into the evolutionary events that have shaped the genomic architecture of various rice species.Lianguang Shang Xiaoxia Li Huiying He Qiaoling Yuan Yanni Song Zhaoran Wei Hai Lin Min Hu Fengli Zhao Chao Zhang Yuhua Li Hongsheng Gao Tianyi Wang Xiangpei Liu Hong Zhang Ya Zhang Shuaimin Cao Xiaoman Yu Bintao Zhang Yong Zhang Yiqing Tan Mao Qin Cheng Ai Yingxue Yang Bin Zhang Zhiqiang Hu Hongru Wang Yang Lv Yuexing Wang Jie Ma Quan Wang Hongwei Lu Zhe Wu Shanlin Liu Zongyi Sun Hongliang Zhang Longbiao Guo Zichao Li Yongfeng Zhou Jiayang Li Zuofeng Zhu Guosheng Xiong Jue Ruan Qian Qian 2022Cell Research2022,32,10:3
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