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| 1 | B7-H3:Another Molecule Marker for Mo-DCs?显示文摘Using a newly generated monoclonal antibody (2E6) against human B7-H3, we explored the expression of the molecule on dendritic cells derived from monocytes (Mo-DCs). Its expression was examined by means of immunostaining and flow cytometric (FCM) analysis. The results showed that B7-H3 was expressed in the course of Mo-DC maturation induced with interleukin 4 (IL-4) and granulocyte/macrophage colony-stimulating factor (GM-CSF). The expression could be detected at all the stages of Mo-DC differentiation, and remained at a quite stable level. Interestingly, B7-H3 was not expressed by T cells and B cells, even these cells were activated respectively by PHA or PWM. A weak expression could be detected on resting monocytes. These data showed that constitutive expression of B7-H3 at a high level was found on imDCs and mDCs derived from monocytes. Due to no expression on T cells and B cells, we speculate that B7-H3 might be another valuable molecule marker for Mo-DCs. | Guangbo Zhang Qiuming Dong Ying Xu Gehua Yu Xueguang Zhang | 2005 | Cellular & Molecular Immunology2005，2，4： | 7 |
| 2 | B7-H3 Over Expression in Prostate Cancer Promotes Tumor Cell Progression显示文摘 | Hexing Yuan Xuedong Wei Guangbo Zhang Chen Li Xueguang Zhang Jianquan Hou | 2011 | The Journal of Urology2011，，3： | 2 |
| 3 | Experimental research on oil fi lm thickness and its microwave scattering during emulsifi cation显示文摘Synthetic Aperture Radar(SAR) plays a major role in identifying oil spills on the sea surface.However,obtaining information of oil spill thickness(volume) is still a challenge.Emulsification is an important process affecting the thickness and normalized radar cross section(NRCS) of oil film.Experiments of crude oil emulsification with C-band fully-polarized scatterometer were conducted combining airborne hyperspectral imaging spectrometer and 3 D laser scanner observation data,to provide experimental parameters and method to support accurate remote sensing monitoring on marine oil spill.It is further proved that through quantitative homogeneous emulsified oil spill experiments,to a certain extent,the NRCS of oil film increased during the emulsification process of crude oil.The backscattering mechanism of crude oil emulsification was explored using a semi-empirical model(SEM);the change of oil film NRCS was modulated by its dielectric constant and surface roughness,in which the dielectric constant showed a dominant effect.The relationship between thickness and NRCS of oil film was studied under two experimental conditions.The differences of NRCS between oil film and adjacent seawater(Δσ~0) and the damping ratio(DR) were found to have a linear relationship with oil thickness,which were best in the vertical polarization mode(VV) at 45° incident angle during the quantitative crude oil homogeneous emulsification process.In the natural emulsification process of continuous oil spill in which oil film was mixed with both crude oil and emulsified oil,an empirical equation of oil film thickness is preliminarily established.The Δσ~0,DR,and the empirical equation of oil film thickness were applied to the marine continuous oil spill incident on a 19-3 oil platform with spaceborne SAR image and successfully explained the distribution of the relative thickness of the oil film. | Jie GUO Chenqi XU Genwang LIU Xi ZHANG Junmin MENG Guangbo REN | 2022 | Journal of Oceanology and Limnology2022，40，4： | 2 |
| 4 | DsbA-DsbAmut fusion chaperon improved soluble expression of human trypsinogen-1 in Escherichia coli显示文摘DsbA-DsbA 傻瓜 第一次被建议提高人的 trypsin-1 的可溶的表达式。作为控制,无领袖的 DsbA 女伴也与人的 trypsin-1 被共同表示。向量 pET39b 胰岛素和 pET28a-DsbADsbA 有上述二个 DsbA 熔化标签的傻瓜 -trypsin 被构造。有向量 pET39b 胰岛素的紧张表示了熔化蛋白质 DsbA 胰岛素处于良好的竟技状态包括身体。当时在 E。有向量 pET28a-DsbA-DsbA 傻瓜 -trypsin, 胰岛素熔化蛋白质的可溶的表示被完成。在优化表示条件下面,可溶的部分占了大约 49.43% 全部的 DsbA-DsbA 在粗略的上层清液的傻瓜 -trypsin 蛋白质。纯化收益由镍 chelating 层析是 4.15% , 3.3 mg 与 88.68% 的纯净激活胰岛素从 1 L 磅肉汤被获得。在胰岛素熔化蛋白质检测 DsbA 系列陪伴的可能的功能,我们分析了熔化蛋白质的主要三维的结构,主要集中于在胰岛素和熔化陪伴之间的兼容。结果建议了那( 1 )除在 periplasm ,无领袖的 DsbA 或 DsbA 傻瓜可以也充当像顺序的领导人指向了到部分从熔化蛋白质 overexpression 和包括身体形成减轻了压力的 periplasm 的一个信号,并且( 2 )因为有 DsbA-DsbA 傻瓜 -trypsin 与DsbA胰岛素相比, DsbA 傻瓜可以在 recombinant 蛋白质 DsbA-DsbA 傻瓜 | Ye Liu Wenyong Zhang Xubin Yang Guangbo Kang Damei Wang He Huang | 2015 | Frontiers of Chemical Science and Engineering2015，9，4： | 2 |
| 5 | Removal of agricultural non-point source pollutants by ditch wetlands: implications for lake eutrophication control显示文摘 | Cuiling Jiang Xiaoqiu Fan Guangbo Cui Yibin Zhang | 2007 | Hydrobiologia2007，，1： | 1 |
| 6 | Morphometric Evaluation of Screw Fixation in Atlas via Posterior Arch and Lateral Mass显示文摘 | Mingsheng Tan Huimin Wang Yunting Wang Guangbo Zhang Ping Yi Zirong Li Hongyu Wei Feng Yang | 2003 | Spine2003，，9： | 1 |
| 7 | Controlled hydrothermal synthesis and magnetic properties of three-dimensional FeSe 2 rod clusters and microspheres显示文摘 | Weidong Shi Xian Zhang Guangbo Che Weiqiang Fan Chunbo Liu | 2013 | Chemical Engineering Journal2013，，： | 1 |
| 8 | B7-H3 was Highly Expressed in Human Primary Hepatocellular Carcinoma and Promoted Tumor Progression显示文摘 | Feifei Wang Guoyan Wang Tongshen Liu Guohua Yu Guangbo Zhang Xiying Luan | 2014 | Cancer Investigation2014，，6： | 1 |
| 9 | Morphometric Evaluation of Screw Fixation in Atlas via Posterior Arch and Lateral Mass显示文摘 | Mingsheng Tan Huimin Wang Yunting Wang Guangbo Zhang Ping Yi Zirong Li Hongyu Wei Feng Yang | 2003 | Spine2003，，9： | 1 |
| 10 | Removal of agricultural non-point source pollutants by ditch wetlands: implications for lake eutrophication control显示文摘 | Cuiling Jiang Xiaoqiu Fan Guangbo Cui Yibin Zhang | 2007 | Hydrobiologia2007，，1： | 1 |
| 11 | P53 codon 72 polymorphism and ovarian cancer risk:a meta-analysis显示文摘客观 p53 是肿瘤 suppressor 基因并且涉及卵巢的癌症的病原学。调查在 72 多型性和卵巢的癌症风险显示出冲突结果的 p53 codon 之间的协会的研究。我们从合格研究执行了这元分析评估这种声称的关系。这元分析从 9 盒子控制研究被执行的方法,包括 825 个卵巢的案例和 1073 控制。他修理了的 T 和随机的效果模型被用来为这多型性的各种各样的对比估计机会比率(ORs ) 。结果联合结果基于所有研究证明显著地减少的风险与变体职业人员 / 职业人员遗传型被联系,与 Arg/Pro+Arg/Arg 遗传型相比(或, 0.70;95%CI, 0.510.95 ) 。当成层时由种族的研究,我们发现有变体遗传型职业人员 / 职业人员的个人与 Arg/Arg 遗传型相比有卵巢的癌症的显著地减少的风险(或, 0.43;95%CI, 0.200.89 ) 并且 Arg/Pro+Arg/Arg 遗传型(或, 0.61;95%CI, 0.370.99 ) 在非洲人之中。结论这元分析建议 72 多型性可以贡献的 p53 codon 基因危险性到卵巢的癌症。更多的研究应该基于更大的样品尺寸被执行证实调查结果。 | Zhizhong Zhang Guangbo Fu Meilin Wang Na Tong Shizhi Wang Zhengdong Zhang | 2008 | Journal of Nanjing Medical University2008，22，5： | 1 |
| 12 | LncRNA HIF1A-AS2 accelerates malignant phenotypes of renal carcinoma by modulating miR-30a-5p/SOX4 axis as a ceRNA显示文摘Objective:Several reports have proposed that lnc RNAs,as potential biomarkers,participate in the progression and growth of malignant tumors.HIF1 A-AS2 is a novel lnc RNA and potential biomarker,involved in the genesis and development of carcinomas.However,the molecular mechanism of HIF1 A-AS2 in renal carcinoma is unclear.Methods:The relative expression levels of HIF1 A-AS2 and miR-30 a-5 p were detected using RT-qPCR in renal carcinoma tissues and cell lines.Using loss-of-function and overexpression,the biological effects of HIF1 A-AS2 and miR-30 a-5 p in kidney carcinoma progression were characterized.Dual luciferase reporter gene analysis and Western blot were used to detect the potential mechanism of HIF1 A-AS2 in renal carcinomas.Results:HIF1 A-AS2 was upregulated in kidney carcinoma tissues when compared with para-carcinoma tissues(P<0.05).In addition,tumor size,tumor node mestastasis stage and differentiation were identified as being closely associated with HIF1 A-AS2 expression(P<0.05).Knockdown or overexpression of HIF1 A-AS2 either restrained or promoted the malignant phenotype and WNT/β-catenin signaling in renal carcinoma cells(P<0.05).Mi R-30 a-5 p was downregulated in renal cancers and partially reversed HIF1 A-AS2 functions in malignant renal tumor cells.HIF1 A-AS2 acted as a micro RNA sponge that actively regulated the relative expression of SOX4 in sponging miR-30 a-5 p and subsequently increased the malignant phenotypes of renal carcinomas.HIF1 A-AS2 showed a carcinogenic effect and miR-30 a-5 p acted as an antagonist of the anti-oncogene effects in the pathogenesis of renal carcinomas.Conclusions:The HIF1 A-AS2-miR-30 a-5 p-SOX4 axis was associated with the malignant progression and development of renal carcinoma.The relative expression of HIF1 A-AS2 was negatively correlated with the expression of miR-30 a-5 p,and was closely correlated with SOX4 mRNA levels in renal cancers. | Mingwei Chen Xuedong Wei Xiu Shi Le Lu Guangbo Zhang Yuhua Huang Jianquan Hou | 2021 | Cancer Biology & Medicine2021，18，2： | 1 |
| 13 | Promotional effects of Sm 2 O 3 on Mn-H 4 SiW 12 O 40 /SiO 2 catalyst for dimethyl ether direct-oxidation to dimethoxymethane显示文摘 | Qingde Zhang Yisheng Tan Guangbo Liu Caihong Yang Yizhuo Han | 2013 | Journal of Industrial and Engineering Chemistry2013，，： | 1 |
| 14 | Diagnosis value of serum B7-H3 expression in non-small cell lung cancer显示文摘 | Guangbo Zhang Yuehua Xu Xudong Lu Haitao Huang Yinghui Zhou Binfeng Lu Xueguang Zhang | 2009 | Lung Cancer2009，，： | 1 |
| 15 | Rapid bioluminescence assay for monitoring rat CES1 activity and its alteration by traditional Chinese medicines显示文摘In traditional Chinese medicine herbs(TCM),including Radix Salviae Miltiorrhizae(Danshen),Radix Puerariae Lobatae(Gegen),Radix Angelicae Sinensis(Danggui),and Rhizoma Chuanxiong(Chuanxiong)are widely used for the prevention and treatment of cardiovascular diseases and also often co-administered with Western drugs as a part of integrative medicine practice.Carboxylesterase 1(CES1)plays a pivotal role in the metabolisms of pro-drugs,Since(S)-2-(2-(6-dimethylamino)-benzothiazole)-4,5-dihydrothiazole-4-carboxylate(NLMe)has recently been identified by us as a selective CES1 bioluminescent sensor,we developed a rapid method using this substrate for the direct measurement of CES1 activity in rats.This bioluminescence assay was applied to determine CES1 activity in rat tissues after a two-week oral administration of each of the four herbs noted above.The results demonstrated the presence of CES1 enzyme in rat blood and all tested tissues with much higher enzyme activity in the blood,liver,kidney and heart than that in the small intestine,spleen,lung,pancreas,brain and stomach.In addition,the four herbs showed tissue-specific effects on rat CES1 expression.Based on the CES1 biodistribution and its changes after treatment in rats,the possibility that Danshen,Gegen and Danggui might alter CES1 activities in human blood and kidney should be considered.In summary,a selective and sensitive bioluminescence assay was developed to rapidly evaluate CES1 activity and the effects of orally administered TCMs in rats. | Jun Zhang Dandan Wang Liwei Zou Min Xiao Yufeng Zhang Ziwei Li Ling Yang Guangbo Ge Zhong Zuo | 2020 | Journal of Pharmaceutical Analysis2020，10，3： | 1 |
| 16 | A Large Range Flexure-Based Servo System Supporting Precision Additive Manufacturing显示文摘 | Zhen Zhang Peng Yan Guangbo Hao | 2017 | Engineering2017，3，5： | 1 |
| 17 | Annual Report of 49-2 SPR in 2017显示文摘1 Information of operation and production Operation person:present chief operators are 5persons,operators are 5persons;new chief operators is 0person,new operator is 2person;training chief operators is 1 persons,training operators are 4persons;transferred chief operator are 1 persons;transferred operator is | ZHANG Yadong YU Jiapei CAI Guangbo | 2017 | Annual Report of China Institute of Atomic Energy2017，，1： | 0 |
| 18 | The hidden risk of microplastic-associated pathogens in aquatic environments显示文摘Increasing studies of plastisphere have raised public concern about microplastics(MPs)as vectors for pathogens,especially in aquatic environments.However,the extent to which pathogens affect human health through MPs remains unclear,as controversies persist regarding the distinct pathogen colonization on MPs as well as the transmission routes and infection probability of MP-associated pathogens from water to humans.In this review,we critically discuss whether and how pathogens approach humans via MPs,shedding light on the potential health risks involved.Drawing on cutting-edge multidisciplinary research,we show that some MPs may facilitate the growth and long-range transmission of specific pathogens in aquatic environments,ultimately increasing the risk of infection in humans.We identify MP-and pathogen-rich settings,such as wastewater treatment plants,aquaculture farms,and swimming pools,as possible sites for human exposure to MP-associated pathogens.This review emphasizes the need for further research and targeted interventions to better understand and mitigate the potential health risks associated with MP-mediated pathogen transmission. | Huan Zhong Mengjie Wu Christian Sonne Su Shiung Lam Raymond W.M.Kwong Yuelu Jiang Xiaoli Zhao Xuemei Sun Xuxiang Zhang Chengjun Li Yuanyuan Li Guangbo Qu Feng Jiang Huahong Shi Rong Ji Hongqiang Ren | 2023 | Eco-Environment & Health2023，2，3： | 0 |
| 19 | Clinical efficacy of low-dose emetine for patients with COVID-19:a real-world study显示文摘Objective:Emetine,an isoquinoline alkaloid that is enriched at high concentrations in the lung,has shown potent in vitro activity against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The aim of this study was to better understand the effectiveness of low-dose emetine for patients with coronavirus disease 2019(COVID-19).Methods:In this real-world study,63 patients with mild or common COVID-19 were recruited from Wuhan Fangcang Shelter Hospital and five COVID-19-designated hospitals in Anhui Province,China from February to March 2020.Thirty-nine patients from Wuhan Fangcang Shelter Hospital were assigned to a pragmatic randomized controlled clinical trial,and 24 patients from the 5 COVID-19-designated hospitals in Anhui Province underwent a real-world study.The medication course of emetine was less than 10 days.The main symptoms and adverse reactions of all patients were observed and recorded.The primary outcome measure was the time required for a negative SARS-CoV-2 RNA result or the negative result rate on day 10.Secondary outcomes included axillary temperature,transcutaneous oxygen saturation,and respiratory frequency recovery.The study was approved by the Ethics Committee of The First Affiliated Hospital of Anhui Medical University on February 20,2019(approval No.PJ2020-03-19)and was registered with the Chinese Clinical Trial Registry on February 20,2019(registration number:ChiCTR2000030022).Results:The oxygen saturation values were higher in the treatment group than in the control group on the first day after enrollment for patients treated at Fangcang Shelter Hospital.The axillary body temperature,respiratory rate,and oxygen saturation among patients in Fangcang Shelter Hospital were related to the time effect but not to the intervention measures.The respiratory rate and oxygen saturation of patients in the Anhui designated hospitals were related to the intervention measures but not to the time effect.The axillary body temperature of patients in Anhui designated hospitals was related to the time effect but not to the intervention measures.Conclusion:Our preliminary study shows that low-dose emetine combined with basic conventional antiviral drugs improves clinical symptoms in patients with mild and common COVID-19 without apparent adverse effects,suggesting that moderately increased doses of emetine may have good potential for treatment and prevention of COVID-19. | Song Fan Qi Zhen Cheng Chen Wenjun Wang Qibing Wu Huihui Ma Chengyuan Zhang Li Zhang Baojing Lu Huiyao Ge Liang Yong Bao Li Yafen Yu Weiwei Chen Yiwen Mao Guangbo Qu Li Su Aoli Wang Zhen Ding Haiwen Li Jin Zhang Yonglian Wang Yufeng Gao Xihai Xu Zhongming Zhu Jun Chen Long Zhang Hongqiang Liang Song Wu Meng Huang Quan Xia Ping Li Yehuan Sun Chaozhao Liang Wei Wei Qingsong Liu Liangdan Sun | 2021 | Journal of Bio-X Research2021，4，2： | 0 |
| 20 | Preventive and therapeutic benefits of nelfinavir in rhesus macaques and human beings infected with SARS-CoV-2显示文摘Effective drugs with broad spectrum safety profile to all people are highly expected to combat COVID-19 caused by SARS-CoV-2.Here we report that nelfinavir,an FDA approved drug for the treatment of HIV infection,is effective against SARS-CoV-2 and COVID-19.Preincubation of nelfinavir could inhibit the activity of the main protease of the SARS-CoV-2(IC50=8.26μM),while its antiviral activity in Vero E6 cells against a clinical isolate of SARS-CoV-2 was determined to be 2.93μM(EC50).In comparison with vehicle-treated animals,rhesus macaque prophylactically treated with nelfinavir had significantly lower temperature and significantly reduced virus loads in the nasal and anal swabs of the animals.At necropsy,nelfinavir-treated animals had a significant reduction of the viral replication in the lungs by nearly three orders of magnitude.A prospective clinic study with 37 enrolled treatment-naive patients at Shanghai Public Health Clinical Center,which were randomized(1:1)to nelfinavir and control groups,showed that the nelfinavir treatment could shorten the duration of viral shedding by 5.5 days(9.0 vs.14.5 days,P=0.055)and the duration of fever time by 3.8 days(2.8 vs.6.6 days,P=0.014)in mild/moderate COVID-19 patients.The antiviral efficiency and clinical benefits in rhesus macaque model and in COVID-19 patients,together with its well-established good safety profile in almost all ages and during pregnancy,indicated that nelfinavir is a highly promising medication with the potential of preventative effect for the treatment of COVID-19. | Zhijian Xu Danrong Shi Jian-Bao Han Yun Ling Xiangrui Jiang Xiangyun Lu Chuan Li Likun Gong Guangbo Ge Yani Zhang Yi Zang Tian-Zhang Song Xiao-Li Feng Ren-Rong Tian Jia Ji Miaojin Zhu Nanping Wu Chunhui Wu Zhen Wang Yechun Xu Cheng Peng Min Zheng Junling Yang Feifei Du Junliang Wu Peipei Wang Jingshan Shen Jianliang Zhang Yong-Tang Zheng Hangping Yao Weiliang Zhu | 2023 | Signal Transduction and Targeted Therapy2023，8，5： | 0 |
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