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| 1 | The basic characteristics and spatial patterns of global cultivated land change since the 1980s显示文摘在这份报纸,我们用全球基于向量的陆地使用 / 登陆盖子数据分析了在 1982 和 2011 之间的栽培陆地变化的空间模式。(1 ) 我们的分析证明全部的全球栽培陆地区域增加了 528.768 吗?? | YAO Ziyan ZHANG Lijuan TANG Shihao LI Xiaxiang HAO Tiantian | 2017 | Journal of Geographical Sciences2017，27，7： | 17 |
| 2 | Regulation of non-classical immune parameters in immune thrombocytopenic purpura mice by a spleen-invigorating,qi-replenishing and blood-containing formula显示文摘Objective:This study investigated the regulatory effect of non-classical immune parameters on immune thrombocytopenic purpura(ITP)mice by a spleen-invigorating,qi-replenishing and blood-containing formula(SQBF).Method:A total of 80 BALB/c mice were randomly divided into four equal groups(20 mice each):control group,model group,prednisone group and spleen-invigorating,qi-replenishing and blood-containing(SQBF)group.Mice in the model group,prednisone group,and SQBF group were administered anti-platelet serum to induce ITP.The dynamic variations of platelet counts in ITP mice were measured with an automatic blood analyzer before modeling and 48 h,and 8,12 and 15 days following APS injection.Levels of b-endorphin(b-EP),vasoactive intestinal peptide(VIP)and salivary IgA(SIgA)were detected by enzyme-linked immunosorbent assay(ELISA)on 15th day of experiment.Results:SQBF enhanced peripheral blood platelet counts in ITP mice similar to that of prednisone,and both groups showed a statistically significant response compared with the model group(P<.01).The SQBF significantly decreased b-EP levels compared with the model and prednisone intervention groups(P<.05),significantly increased the levels of VIP and SIgA in ITP mice compared with the model group(P<.05)and had significant protective effects on the thymus of ITP mice compared with the model group(P<.01).Conclusions:The SQBF had a similar effect to prednisone with regards to enhancing peripheral blood platelet counts in ITP mice.Furthermore,it decreased b-EP levels and increased VIP and SIgA,and protected the thymus.This shows that,on base of the brain-gut axis functions,some non-classical immune vascular active factors or neurotransmitters are also involved in immune responses,and also have relationship with the onset of ITP and bleeding and/or hemostasis.It needs further study to determine whether a change in these active factors is related to immediate hemostasis. | Tiantian Li Hao He Li Hou Yue Xu Xiaoyong Wu Yanping Sun Lei Zheng Zhixiong Chen Xinyi Chen Bei Qin | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，2： | 6 |
| 3 | Study on Local Resistance of non-Newtonian Power Law Fluid in Elbow Pipes显示文摘This paper focuses on the flow characteristic and local resistance of non-Newtonian power law fluid in a curved90° bend pipe with circular cross-sections,which are widely used in industrial applications.By employing numerical simulation and theoretical analysis the properties of the flow and local resistance of power law fluid under different working conditions are obtained.To explore the change rule the experiment is carried out by changing the Reynolds number,the wall roughness and different diameter ratio of elbow pipe.The variation of the local resistance coefficient with the Reynolds number,the diameter ratio and the wall roughness is presented comprehensively in the paper.The results show that the local resistance force coefficient hardly changes with Reynolds number of the power law fluid;the wall roughness has a significant impact on the local resistance coefficient.As the pipe wall roughness increasing,the coefficient of local resistance force will increase.The main reason of the influence of the roughness on the local resistance coefficient is the increase of the eddy current region in the power law fluid flow,which increases the kinetic energy dissipation of the main flow.This paper provides theoretical and numerical methods to understand the local resistance property of non-Newtonian power law fluid in elbow pipes. | ZHANG Hao XU Tiantian ZHANG Xinxin WANG Yuxiang WANG Yuancheng LIU Xueting | 2016 | Journal of Thermal Science2016，25，3： | 4 |
| 4 | Clinical Predictors of Functional Cure in Children 1–6 Years-old with Chronic Hepatitis B显示文摘Background and Aims:Hepatitis B surface antigen(HBsAg)clearance is significantly more common in children with chronic hepatitis B(CHB)than in adults;however,the possible influencing factors related to HBsAg loss have yet to be found.This study aimed to explore the efficacy of long-term interferon(IFN)αtherapy in treating children with CHB and analyzed the factors influencing functional cure after treatment.Methods:A total of 236 children aged 1–6 years and diagnosed with CHB via liver biopsy were included in the study,all receiving IFNαtreatment(IFNα-2b monotherapy,IFNα-2b followed by lamivudine[LAM]or IFNα-2b combined with LAM)and followed up for 144 weeks.A comprehensive analysis was conducted on clinical data,including biochemical items,serum markers of hepatitis B virus(HBV)and immunological indexes,and logistic regression analysis was used to screen the influencing factors related to HBsAg loss.Results:The cumulative loss rates of HBsAg were 79.5%,62.1%and 42.1%at 144 weeks after the start of treatment in the 1–3 years-old group,3–5 years-old group and 5–7 years-old group,respectively(p<0.05).IFNα-2b combined with LAM treatment displayed the highest HBsAg loss rates compared with monotherapy and sequential treatment(p=0.011).Younger baseline age and lower HBsAg levels were independent factors for the prediction of HBsAg loss(p<0.05).The baseline PreS1 and hepatitis B core antibody levels in the HBsAg loss group were lower than those in the HBsAg non-loss group.In addition,the PreS1 level was positively corelated with the level of HBsAg,HBV DNA and liver inflammation.Conclusions:Long-term treatment with IFNαwas effective in achieving HBsAg loss in CHB children aged 1–6 years-old.Age less than 3 years-old and lower HBsAg levels are independent predictors of functional cure in children with CHB. | Jing Pan Haiyan Wang Tiantian Yao Xuejiao Liao Hao Cheng Suthat Liangpunsakul Yan Wang Min Zhang Zheng Zhang | 2022 | Journal of Clinical and Translational Hepatology2022，10，3： | 3 |
| 5 | Controllable self-assembly of parallel gold nanorod clusters by DNA origami显示文摘Herein we demonstrate the construction of three types of parallel gold nanorod(AuNR) clusters using a DNA origami rod(DOR) as the template. Based on the precise control over the position of capture strands on DOR, number and orientation of the AuNR clusters can be well engineered, as evidenced by biological transmission electron microscope(TEM). Importantly, the AuNR clusters exhibit chiroptical responses which are strongly affected by the number of AuNR on rod-like DNA origami. | Hang Yu Tiantian Man Wei Ji Leilei Shi Chenwei Wu Hao Pei Chuan Zhang | 2019 | Chinese Chemical Letters2019，30，1： | 2 |
| 6 | AaWRKY17, a positive regulator of artemisinin biosynthesis, is involved in resistance to Pseudomonas syringae in Artemisia annua显示文摘Artemisia annua,a traditional Chinese medicinal plant,remains the only plant source for artemisinin production,yet few genes have been identified to be involved in both the response to biotic stresses,such as pathogens,and artemisinin biosynthesis.Here,we isolated and identified the WRKY transcription factor(TF)AaWRKY17,which could significantly increase the artemisinin content and resistance to Pseudomonas syringae in A.annua.Yeast one-hybrid(Y1H),dual-luciferase(dual-LUC),and electrophoretic mobility shift assay(EMSA)results showed that AaWRKY17 directly bound to the W-box motifs in the promoter region of the artemisinin biosynthetic pathway gene amorpha-4,11-diene synthase(ADS)and promoted its expression.Real-time quantitative PCR(RT-qPCR)analysis revealed that the transcript levels of two defense marker genes,Pathogenesis-Related 5(PR5)and NDR1/HIN1-LIKE 10(NHL10),were greatly increased in AaWRKY17-overexpressing transgenic A.annua plants.Additionally,overexpression of AaWRKY17 in A.annua resulted in decreased susceptibility to P.syringae.These results indicated that AaWRKY17 acted as a positive regulator in response to P.syringae infection.Together,our findings demonstrated that the novel WRKY transcription factor AaWRKY17 could potentially be used in transgenic breeding to improve the content of artemisinin and pathogen tolerance in A.annua. | Tiantian Chen Yongpeng Li Lihui Xie Xiaolong Hao Hang Liu Wei Qin Chen Wang Xin Yan Kuanyu Wu-Zhang Xinghao Yao Bowen Peng Yaojie Zhang Xueqing Fu Ling Li Kexuan Tang | 2021 | Horticulture Research2021，8，1： | 2 |
| 7 | An assessment of tradi-tional uighur medicine in current Xinjiang region(China) 显示文摘 | Ma Zhiqiao Hu Hao He Tiantian | 2014 | Afr J Tradit Complement Altem Med2014，11，2： | 1 |
| 8 | Ternary ZnO/Co_(3)O_(4)/NiO inherited layered core-shell structure from a double template for high performanced supercapacitor显示文摘Relying on seed method,a double template ZIF-8@ZIF-67 with a layered core-shell structure was made,which is used as a sacrificial template in the next steps.After going through loading process with solvent thermal method and calcination process,ZIF-8@ZIF-67 was etched and calcined into ternary ZnO/Co_(3)O_(4)/NiO successfully.Besides,benefiting from right amount of the addition of inorganic salts,the optimized ZnO/Co_(3)O_(4)/NiO-2(ZCN-2)shows the outstanding performance in its morphologic structure and electrochemical performance.In the case of a current density of 1 Ag^(-1),the high specific capacitance of 1119.11 Cg^(-1) can be calculated in ZCN-2.In addition,at 10 Ag^(-1),the capacitance value can maintain 93.75%after experiencing 5000 cycles of charging and discharging.The maximum energy density of the assembled ZCN-2//activated carbon(AC)asymmetric supercapacitor can reach 95.82 Wh kg^(-1),which is really outstanding in the present research achievements.After experiencing charge-discharge cycles with the number of 10,000 times in the case of a current density of 10 Ag^(-1),the cycling stability of this supercapacitor can reach 94.53%of the initial value.And after a charge,the device connected by two supercapacitors can make a light emitting diode(LED)shine up to 30 min successfully. | Linli Zhu Chen Hao Saisai Zhou Xiaohong Wang Tiantian Zhou Yaning Guo | 2021 | Journal of Materiomics2021，7，4： | 1 |
| 9 | An Adversarial Smart Contract Honeypot in Ethereum显示文摘A smart contract honeypot is a special type of smart contract.This type of contract seems to have obvious vulnerabilities in contract design.If a user transfers a certain amount of funds to the contract,then the user can withdraw the funds in the contract.However,once users try to take advantage of this seemingly obvious vulnerability,they will fall into a real trap.Consequently,the user’s investment in the contract cannot be retrieved.The honeypot induces other accounts to launch funds,which seriously threatens the security of property on the blockchain.Detection methods for honeypots are available.However,studying the manner by which to defend existing honeypots is insufficient to fight against honeypots.The new honeypots that may appear in the future from the perspective of an attacker must also be predicted.Therefore,we propose a type of adversarial honeypot.The code and behavioral features of honeypots are obtained through a comparative analysis of the 158,568 nonhoneypots and 352 honeypots.To build an adversarial honeypot,we try to separately hide these features and make the honeypot bypass the existing detection technology.We construct 18 instances on the basis of the proposed adversarial honeypot and use an open-source honeypot detection tool to detect these instances.The experimental result shows that the proposed honeypot can bypass the detection tool with a 100%ratio.Therefore,this type of honeypot should be given attention,and defensive measures should be proposed as soon as possible. | Yu Han Tiantian Ji Zhongru Wang Hao Liu Hai Jiang Wendi Wang Xiang Cui | 2021 | Computer Modeling in Engineering & Sciences2021，，7： | 1 |
| 10 | Gefitinib and fostamatinib target EGFR and SYK to attenuate silicosis:a multi-omics study with drug exploration显示文摘Silicosis is the most prevalent and fatal occupational disease with no effective therapeutics,and currently used drugs cannot reverse the disease progress.Worse still,there are still challenges to be addressed to fully decipher the intricated pathogenesis.Thus,specifying the essential mechanisms and targets in silicosis progression then exploring anti-silicosis pharmacuticals are desperately needed.In this work,multi-omics atlas was constructed to depict the pivotal abnormalities of silicosis and develop targeted agents.By utilizing an unbiased and time-resolved analysis of the transcriptome,proteome and phosphoproteome of a silicosis mouse model,we have verified the significant differences in transcript,protein,kinase activity and signaling pathway level during silicosis progression,in which the importance of essential biological processes such as macrophage activation,chemotaxis,immune cell recruitment and chronic inflammation were emphasized.Notably,the phosphorylation of EGFR(p-EGFR)and SYK(pSYK)were identified as potential therapeutic targets in the progression of silicosis.To inhibit and validate these targets,we tested fostamatinib(targeting SYK)and Gefitinib(targeting EGFR),and both drugs effectively ameliorated pulmonary dysfunction and inhibited the progression of inflammation and fibrosis.Overall,our drug discovery with multi-omics approach provides novel and viable therapeutic strategies for the treatment of silicosis. | Mingyao Wang Zhe Zhang Jiangfeng Liu Meiyue Song Tiantian Zhang Yiling Chen Huiyuan Hu Peiran Yang Bolun Li Xiaomin Song Junling Pang Yanjiang Xing Zhujie Cao Wenjun Guo Hao Yang Jing Wang Juntao Yang Chen Wang | 2022 | Signal Transduction and Targeted Therapy2022，7，6： | 1 |
| 11 | Comparative Proteomic Analysis of Cucumber Fruits Under Nitrogen Deficiency At the Fruiting Stage显示文摘Nitrogen deficiency is a major factor that affects the yield and quality of horticultural crops.Differentially expressed proteins were identified in cucumber fruit under nitrogen deficiency treatment,and are expected to be highly useful for dissecting carbon,nitrogen and ascorbate metabolism in cucumber and for improving nitrogen fertilizer utilization.Cucumber plants grown hydroponically in Hoagland solution were transferred to nitrogen-free conditions for 3 or 5 d after the blossom of female flowers.Fruit proteome profiles were assessed using twodimensional electrophoresis.The relative expression of the genes encoding 50 selected differentially expressed proteins and 4 key enzymes in carbon metabolism was analyzed by qRT-PCR.Resulted showed that most of the identified differentially expressed proteins were mainly associated with carbon metabolism,amino acid biosynthesis,ascorbate metabolism,and proteasomes.Glucose phosphorylation was enhanced,while the pentose phosphate pathway was inhibited.Carbon metabolism and the synthesis of most amino acids was affected significantly,and ascorbate might be accumulated under nitrogen deficiency in cucumber fruit. | Jia Hao Qiang Li Hongjun Yu Heng Wang Lin Chai Tiantian Miao Weijie Jiang | 2021 | Horticultural Plant Journal2021，7，1： | 0 |
| 12 | Effects of Root Cutting on Morphological Characteristics and Endogenous Hormone Levels of Quercus variabilis Seedlings显示文摘The objective of this study was to investigate the effects of root cutting stress on the dynamic changes of endogenous hormone content and growth characteristics of Quercus variabilis roots,and to explore the physiological role of endogenous hormones in regulating root-crown interactions in the short term.The morphological characteristics and endogenous hormone contents of normal roots(no root cutting,CK)and cut roots(cut by 1/3 of the length of the main root,RP)were determined by liquid chromatography,which was combined with mass spectrometry at different levels of different developmental stages.The results showed that the root growth indexes and root endogenous hormones in the RP group were superior to those in the CK group.Through comprehensive analysis of endogenous hormones,it was found that the crosstalk of IAA,JA,ABA and SA could activate the root growth defense.After the root cutting treatment,the root growth of Quercus variabilis seedlings could compensate for the inhibition of taproot growth by promoting lateral root growth.The growth and development of compensatory lateral roots contribute to the increase the total root length of plants,thus promoting the absorption of water and nutrients.It is speculated that plant hormones may be the key factors affecting their development,but this is not only related to the content of a single hormone,but more importantly,it is the interaction between various hormones. | Ruoyi Ni Hao Dou Shuwen Bi Tiantian Wang Jin’e Quan Xitian Yang | 2023 | Phyton-International Journal of Experimental Botany2023，92，10： | 0 |
| 13 | Reflectin基因起源及其蛋白质逐级组装的性质显示文摘文章简介在自然界中,头足纲动物以动态变色和拟态隐形而著称。这其中涉及两种类型的颜色,分别是色素色彩和结构色彩。Reflectin是一类只存在于头足纲动物体内的特殊蛋白质家族,其构成了它们结构色变化的物质基础,然而其基因来源以及如何介导结构色彩变化的分子机理尚不清楚。 | Zhe Guan Tiantian Cai Zhongmin Liu Yunfeng Dou Xuesong Hu Peng Zhang Xin Sun Hongwei Li Yao Kuang Qiran Zhai Hao Ruan Xuanxuan Li Zeyang Li Qihui Zhu Jingeng Mai Qining Wang Luhua Lai Jianguo Ji Haiguang Liu Bin Xia Taijiao Jiang Shu-Jin Luo Hong-Wei Wang 谢灿 | 2018 | 科学新闻2018，0，4： | 0 |
| 14 | Detection and classification of breast lesions using multiple information on contrast-enhanced mammography by a multiprocess deep-learning system: A multicenter study显示文摘Objective: Accurate detection and classification of breast lesions in early stage is crucial to timely formulate effective treatments for patients. We aim to develop a fully automatic system to detect and classify breast lesions using multiple contrast-enhanced mammography(CEM) images.Methods: In this study, a total of 1,903 females who underwent CEM examination from three hospitals were enrolled as the training set, internal testing set, pooled external testing set and prospective testing set. Here we developed a CEM-based multiprocess detection and classification system(MDCS) to perform the task of detection and classification of breast lesions. In this system, we introduced an innovative auxiliary feature fusion(AFF)algorithm that could intelligently incorporates multiple types of information from CEM images. The average freeresponse receiver operating characteristic score(AFROC-Score) was presented to validate system’s detection performance, and the performance of classification was evaluated by area under the receiver operating characteristic curve(AUC). Furthermore, we assessed the diagnostic value of MDCS through visual analysis of disputed cases,comparing its performance and efficiency with that of radiologists and exploring whether it could augment radiologists’ performance.Results: On the pooled external and prospective testing sets, MDCS always maintained a high standalone performance, with AFROC-Scores of 0.953 and 0.963 for detection task, and AUCs for classification were 0.909[95% confidence interval(95% CI): 0.822-0.996] and 0.912(95% CI: 0.840-0.985), respectively. It also achieved higher sensitivity than all senior radiologists and higher specificity than all junior radiologists on pooled external and prospective testing sets. Moreover, MDCS performed superior diagnostic efficiency with an average reading time of 5 seconds, compared to the radiologists’ average reading time of 3.2 min. The average performance of all radiologists was also improved to varying degrees with MDCS assistance.Conclusions: MDCS demonstrated excellent performance in the detection and classification of breast lesions,and greatly enhanced the overall performance of radiologists. | Yuqian Chen Zhen Hua Fan Lin Tiantian Zheng Heng Zhou Shijie Zhang Jing Gao Zhongyi Wang Huafei Shao Wenjuan Li Fengjie Liu Simin Wang Yan Zhang Feng Zhao Hao Liu Haizhu Xie Heng Ma Haicheng Zhang Ning Mao | 2023 | Chinese Journal of Cancer Research2023，35，4： | 0 |
| 15 | The First Crested Duck Genome Reveals Clues to Genetic Compensation and Crest Cushion Formation显示文摘The Chinese crested(CC)duck is a unique indigenous waterfowl breed,which has a crest cushion that affects its survival rate.Therefore,the CC duck is an ideal model to investigate the genetic compensation response to maintain genetic stability.In the present study,we first generated a chromosome-level genome of CC ducks.Comparative genomics revealed that genes related to tissue repair,immune function,and tumors were under strong positive selection,indicating that these adaptive changes might enhance cancer resistance and immune response to maintain the genetic stability of CC ducks.We also assembled a Chinese spot-billed(Csp-b)duck genome,and detected the structural variations(SVs)in the genome assemblies of three ducks(i.e.,CC duck,Csp-b duck,and Peking duck).Functional analysis revealed that several SVs were related to the immune system of CC ducks,further strongly suggesting that genetic compensation in the anti-tumor and immune systems supports the survival of CC ducks.Moreover,we confirmed that the CC duck originated from the mallard ducks.Finally,we revealed the physiological and genetic basis of crest traits and identified a causative mutation in TAS2R40 that leads to crest formation.Overall,the findings of this study provide new insights into the role of genetic compensation in adaptive evolution. | Guobin Chang Xiaoya Yuan Qixin Guo Hao Bai Xiaofang Cao Meng Liu Zhixiu Wang Bichun Li Shasha Wang Yong Jiang Zhiquan Wang Yang Zhang Qi Xu Qianqian Song Rui Pan Lingling Qiu Tiantian Gu Xinsheng Wu Yulin Bi Zhengfeng Cao Yu Zhang Yang Chen Hong Li Jianfeng Liu Wangcheng Dai Guohong Chen | 2023 | Genomics, Proteomics & Bioinformatics2023，21，3： | 0 |
| 16 | Evaluation of an innovative pediatric isolation(PI)bed using fluid dynamics simulation and aerosol isolation efficacy显示文摘Airborne transmission is an important mechanism of spread for both viruses and bacteria in hospitals,with nosocomial infections putting a great burden on public health.In this study,we designed and manufactured a bed for pediatric clinic consultation rooms providing air isolation to protect patients and medical personnel from pathogen transmission.The pediatric isolation bed has several primary efficiency filters and a high-efficiency particulate air filter in the bedside unit.The air circulation between inlet and outlet forms negative pressure to remove the patient's exhaled air timeously and effectively.A computational fluid dynamics model was used to calculate the speed of the airflow and the angle of sampler.Following this,we conducted purification experiments using cigarette smoke,Staphylococcus albus(S.albus)and human adenovirus type 5(HAdV-5)to demonstrate the isolation efficacy.The results showed that the patient's head should be placed as close to the air inlet hood as possible,and an air intake wind speed of 0.86 m/s was effective.The isolation efficacy of the pediatric isolation bed was demonstrated by computational fluid dynamics technology.The isolation efficiency against cigarette smoke exceeded 91.8%,and against S.albus was greater than 99.8%,while the isolation efficiency against HAdV-5 was 100%.The pediatric isolation bed could be used where isolation wards are unavailable,such as in intensive care units and primary clinical settings,to control hospital acquired infections. | Tiantian Liu Yubing Guo Xiaotang Hao Mei Wang Shicong He Zhengshi Lin Rong Zhou | 2021 | Building Simulation2021，14，5： | 0 |
| 17 | Global Solutions of 3-D Inhomogeneous Navier-Stokes System with Large Viscosity in One Variable显示文摘We consider the global well-posedness of three dimensional incompressible inhomogeneous Navier-Stokes equation with different viscous coefficients in the vertical and horizontal variables.In particular,when one of these viscous coefficients is large enough compared with the initial data and the initial density is close enough to a positive constant,we prove the global wellposedness of this system.This result extends the previous results in[9,11]for the classical Navier Stokes system. | Tiantian Hao | 2022 | Communications in Mathematical Research2022，38，1： | 0 |
| 18 | Autoinhibitory structure of preligand association state implicates a new strategy to attain effective DR5 receptor activation显示文摘Members of the tumor necrosis factor receptor superfamily(TNFRSF)are important therapeutic targets that can be activated to induce death of cancer cells or stimulate proliferation of immune cells.Although it has long been implicated that these receptors assemble preligand associated states that are required for dominant interference in human disease,such states have so far eluded structural characterization.Here,we find that the ectodomain of death receptor 5(DR5-ECD),a representative member of TNFRSF,can specifically self-associate when anchored to lipid bilayer,and we report this self-association structure determined by nuclear magnetic resonance(NMR).Unexpectedly,two non-overlapping interaction interfaces are identified that could propagate to higher-order clusters.Structure-guided mutagenesis indicates that the observed preligand association structure is represented on DR5-expressing cells.The DR5 preligand association serves an autoinhibitory role as single-domain antibodies(sdAbs)that partially dissociate the preligand cluster can sensitize the receptor to its ligand TRAIL and even induce substantial receptor signaling in the absence of TRAIL.Unlike most agonistic antibodies that require multivalent binding to aggregate receptors for activation,these agonistic sdAbs are monovalent and act specifically on an oligomeric,autoinhibitory configuration of the receptor.Our data indicate that receptors such as DR5 can form structurally defined preclusters incompatible with signaling and that true agonists should disrupt the preligand cluster while converting it to signaling-productive cluster.This mechanism enhances our understanding of a long-standing question in TNFRSF signaling and suggests a new opportunity for developing agonistic molecules by targeting receptor preligand clustering. | Gang Du Linlin Zhao Yumei Zheng Anissa Belfetmi Tiantian Cai Boying Xu Karen Heyninck Kim Van Den Heede Marie-Ange Buyse Pietro Fontana Michael Bowman Lih-Ling Lin Hao Wu James Jeiwen Chou | 2023 | Cell Research2023，33，2： | 0 |
| 19 | GRK2 mediated degradation of SAV1 initiates hyperplasia of fibroblast-like synoviocytes in rheumatoid arthritis显示文摘Hyperplasia and migration of fibroblast-like synoviocytes(FLSs)are the key drivers in the pathogenesis of rheumatoid arthritis(RA)and joint destruction.Abundant Yes-associated protein(YAP),which is a powerful transcription co-activator for proliferative genes,was observed in the nucleus of inflammatory FLSs with unknown upstream mechanisms.Using Gene Expression Omnibus database analysis,it was found that Salvador homolog-1(SAV1),the pivotal negative regulator of the Hippo-YAP pathway,was slightly downregulated in RA synovium.However,SAV1 protein expression is extremely reduced.Subsequently,it was revealed that SAV1 is phosphorylated,ubiquitinated,and degraded by interacting with an important serine-threonine kinase,G protein-coupled receptor(GPCR)kinase 2(GRK2),which was predominately upregulated by GPCR activation induced by ligands such as prostaglandin E2(PGE2)in RA.This process further contributes to the decreased phosphorylation,nuclear translocation,and transcriptional potency of YAP,and leads to aberrant FLSs proliferation.Genetic depletion of GRK2 or inhibition of GRK2 by paroxetine rescued SAV1 expression and restored YAP phosphorylation and finally inhibited RA FLSs proliferation and migration.Similarly,paroxetine treatment effectively reduced the abnormal proliferation of FLSs in a rat model of collagen-induced arthritis which was accompanied by a significant improvement in clinical manifestations.Collectively,these results elucidate the significance of GRK2 regulation of Hippo-YAP signaling in FLSs proliferation and migration and the potential application of GRK2 inhibition in the treatment of FLSs-driven joint destruction in RA. | Paipai Guo Ji Jiang Rui Chu Feng He Mingli Ge Ruhong Fang Qiuyun Guan Huijuan Cheng Chunru Jiang Tiantian Su Zhenduo Zhu Hao Liu Wei Wei Shihao Zhang Qingtong Wang | 2024 | Acta Pharmaceutica Sinica B2024，14，3： | 0 |
| 20 | Histones of Neutrophil Extracellular Traps Induce CD11b Expression in Brain Pericytes Via Dectin-1 after Traumatic Brain Injury显示文摘The brain pericyte is a unique and indispensable part of the blood-brain barrier(BBB),and contributes to several pathological processes in traumatic brain injury(TBI).However,the cellular and molecular mechanisms by which pericytes are regulated in the damaged brain are largely unknown.Here,we show that the formation of neutrophil extracellular traps(NETs)induces the appearance of CD11b^(+)pericytes after TBI.These CD11b^(+)pericyte subsets are characterized by increased permeability and pro-inflammatory profiles compared to CD11b-pericytes.Moreover,histones from NETs by Dectin-1 facilitate CD11b induction in brain pericytes in PKC-c-Jun dependent manner,resulting in neuroinflammation and BBB dysfunction after TBI.These data indicate that neutrophil-NET-pericyte and histone-Dectin-1-CD11b are possible mechanisms for the activation and dysfunction of pericytes.Targeting NETs formation and Dectin-1 are promising means of treating TBI. | Yang-Wuyue Liu Jingyu Zhang Wanda Bi Mi Zhou Jiabo Li Tiantian Xiong Nan Yang Li Zhao Xing Chen Yuanguo Zhou Wenhui He Teng Yang Hao Wang Lunshan Xu Shuang-Shuang Dai | 2022 | Neuroscience Bulletin2022，38，10： | 0 |
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