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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Modeling tree root diameter and biomass by ground-penetrating radar显示文摘Roots play a key role in ecosystem functioning as they transfer water and nutrients from soil to plants.Traditional methods for measuring roots are labor-intensive and destructive in nature,which limits quantitative and repeatable assessments in longterm research.Ground-penetrating radar(GPR) provides a non-destructive method to measure plant roots.Based on the superiority of GPR with 2 GHz frequency,we developed a new,practical method to estimate root biomass.First,average root matter density was measured by collecting a small number of root samples.Second,under controlled,experimental conditions in a sandy area,a root diameter estimation model base on GPR was developed from which root diameter was estimated.Third,root volume was calculated using the estimated root diameter and assuming the shape of roots to be cylindrical.Finally,root biomass was estimated by averaging root matter density and root volume.Results of this study suggest the following:(1) the density of coarse roots with diameters greater than 0.5 cm is relatively uniform;(2) a new wave shape parameter,ΔT,extracted from profile data of 2 GHz frequency antenna is independent of root depth,thus enabling the construction of a root diameter estimation model with high accuracy;and(3) results of a field experiment demonstrated the GPR-based method to be feasible and effective in estimating biomass of coarse roots.These findings are helpful for improving GPR-based root diameter and biomass estimation models and suggest the potential of GPR data in studying root systems. | CUI XiHong CHEN Jin SHEN JinSong CAO Xin CHEN XueHong ZHU XiaoLin | 2011 | Science China Earth Sciences2011，54，5： | 8 |
| 3 | Circular SAR processing using an improved omega-k type algorithm显示文摘An improved circular synthetic aperture radar(CSAR) imaging algorithm of omega-k(ω-k) type mainly for reconstructing an image on a cylindrical surface is proposed.In the typical CSAR ω-k algorithm,the rage trajectory is approximated by Taylor series expansion to the quadratic terms,which limits the valid synthetic aperture length and the angular reconstruction range severely.Based on the model of the CSAR echo signal,the proposed algorithm directly transforms the signal to the two-dimensional(2D) wavenumber domain,not using approximation processing to the range trajectory.Based on form of the signal spectrum in the wavenumber domain,the formula for the wavenumber domain interpolation of the w-k algorithm is deduced,and the wavenumber spectrum of the reference point used for bulk compression is obtained from numerical method.The improved CSAR ω-k imaging algorithm increases the valid synthetic aperture length and the angular area greatly and hence improves the angular resolution of the cylindrical imaging.Additionally,the proposed algorithm can be repeated on different cylindrical surfaces to achieve three dimensional(3D) image reconstruction.The 3D spatial resolution of the CSAR system is discussed,and the simulation results validate the correctness of the analysis and the feasibility of the algorithm. | Leilei KOU Xiaoqing Wang Jinsong Chong Maosheng Xiang Minhui Zhu | 2010 | Journal of Systems Engineering and Electronics2010，21，4： | 7 |
| 4 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 5 | MATRIEX imaging:multiarea two-photon real-time in vivo explorer显示文摘Two-photon laser scanning microscopy has been extensively applied to study in vivo neuronal activity at cellular and subcellular resolutions in mammalian brains.However,the extent of such studies is typically confined to a single functional region of the brain.Here,we demonstrate a novel technique,termed the multiarea two-photon real-time in vivo explorer(MATRIEX),that allows the user to target multiple functional brain regions distributed within a zone of up to 12mm in diameter,each with a field of view(FOV)of ~200μm in diameter,thus performing two-photon Ca2+imaging with single-cell resolution in all of the regions simultaneously.For example,we demonstrate real-time functional imaging of single-neuron activities in the primary visual cortex,primary motor cortex and hippocampal CA1 region of mice in both anesthetized and awake states.A unique advantage of the MATRIEX technique is the configuration of multiple microscopic FOVs that are distributed in three-dimensional space over macroscopic distances(>1 mm)both laterally and axially but that are imaged by a single conventional laser scanning device.In particular,the MATRIEX technique can be effectively implemented as an add-on optical module for an existing conventional single-beam-scanning two-photon microscope without requiring any modification to the microscope itself.Thus,the MATRIEX technique can be readily applied to substantially facilitate the exploration of multiarea neuronal activity in vivo for studies of brain-wide neural circuit function with single-cell resolution. | Mengke Yang Zhenqiao Zhou Jianxiong Zhang Shanshan Jia Tong Li Jiangheng Guan Xiang Liao Bing Leng Jing Lyu Kuan Zhang Min Li Yan Gong Zhiming Zhu Junan Yan Yi Zhou Jian K Liu Zsuzsanna Varga Arthur Konnerth Yuguo Tang Jinsong Gao Xiaowei Chen Hongbo Jia | 2019 | Light(Science & Applications)2019，8，1： | 4 |
| 6 | Oxidative damage of primary cultured hippocampal neurons Does androgen have an antagonistic effect?显示文摘BACKGROUND: Evidence illustrates that androgen has a neuroprotective role.However,whether androgen also has the protective effect on hippocampal neurons during free radical mediated injury remains unclear.OBJECTIVE: To investigate the neuroprotective effect of androgen on hippocampal neurons during free radical damage.DESIGN,TIME AND SETTING: A controlled in vitro experiment was performed at the Department of Human Anatomy,Cell Culture Lab,and Neuroendocrinology Lab,Basic Medical School,Hebei Medical University from February to June 2009.MATERIALS: Testosterone was provided by Tianjin Jinyao Amino Acid Company,China.METHODS: Primary cultured neurons from 24 Sprague Dawley rats were randomly assigned into four groups: control,H2O2,testosterone,and testosterone (pre-added) plus H2O2 groups.MAIN OUTCOME MEASURES: The positive cell ratio of microtubule associated protein-II and neuron specific enolase was determined by immunocytochemistry.Neuronal morphology was observed by hematoxylin-eosin staining and Nissl staining.Cell vitality and viability were determined using an inverted phase contrast microscope.The content of nitric oxide synthase,malondialdehyde,and superoxide dismutase were measured with a spectrophotometer.RESULTS: As compared with the control group,cell vitality and viability,and superoxide dismutase level were significantly decreased in the H2O2 group (P < 0.05),while nitric oxide synthase and malondialdehyde levels were significantly increased (P < 0.05).Neuronal vitality and viability as well as superoxide dismutase level in the testosterone plus H2O2 group were significantly greater than in the H2O2 group (P < 0.05),and nitric oxide synthase and malondialdehyde levels were significantly less than in the H2O2 group (P < 0.05).CONCLUSION: Androgen partially reversed H2O2-induced neuronal damage and protected neurons. | Zhaohui Li Zhiping Cai Huixian Cui Jinsong Zhu Sha Li Guosheng Xie Lei Xue | 2010 | Neural Regeneration Research2010，5，5： | 4 |
| 7 | Starch RVA profile parameters of rice are mainly controlled by Wx gene显示文摘Quantitative trait locus (QTLs) mapping for rapid visco analyser (RVA) profile parameters has been carried out by using a double haploid (DH) population derived from a cross between indica variety Zhai-Ye-Qing 8 and j’aponica variety Jing-Xi 17 and its genetic linkage map. The results indicate that the segregation of the RVA profiles is continually distributed a-mong the DH lines, and some DH lines show transgressive segregation for all the parameters. | Jinsong Bao Ping He Yingwu Xia Ying Chen Lihuang Zhu | 1999 | Chinese Science Bulletin1999，44，22： | 3 |
| 8 | RESEARCH ON NEGOTIATION-BASED PARTNER SELECTION APPROACH显示文摘The key problem in the construction of virtual enterprises (VEs) is how to select appropriate partners. The negotiation-based approach is proposed to support partner selection in the construction of VEs . The negotiation model is discussed from three main aspects respectively, i.e., negotiation protocol, negotiation goal and negotiation decision-making model. And the generic mathematical description of the negotiation model is formally presented. Finally, a simple example is used to validate the approach’s availability. | Li Li Xue Jinsong Zhu Yunlong Shenyang Institute of Automation,Chinese Academy of Science,Shenyang 110016,China | 2002 | Chinese Journal of Mechanical Engineering2002，15，1： | 3 |
| 9 | Athermal 4-channel (de-)multiplexer in silicon nitride fabricated at low temperature显示文摘We have designed and realized an athermal 4-channel wavelength(de-)multiplexer in silicon nitride(Si N).Minimized thermal sensitivity is achieved in a wide wavelength range by using wide and narrow waveguides with low and different thermal-optic coefficients in the two arms of Mach–Zehnder interferometers(MZIs). The Si N core layer and Si O2 cladding layers are deposited by a low-temperature plasma-enhanced chemical vapor deposition process. The fabricated MZI filter exhibits a thermal sensitivity within 2.0 pm∕°C in a wavelength range of 55 nm to near 1300 nm. Then, an athermal(de-)multiplexer based on cascaded MZIs has been demonstrated with a crosstalk ≤-22 d B and a thermal sensitivity <4.8 pm∕°C for all four channels, reduced by 77% compared to a conventional Si N(de-)multiplexer. Owing to the passive operation and compatibility with the CMOS backend process, our devices have potential applications in 3 D integration of photonics and electronics. | SHIQI TAO QINGZHONG HUANG LIANGQIU ZHU JUN LIU YINGLU ZHANG YING HUANG YI WANG JINSONG XIA | 2018 | Photonics Research2018，6，7： | 3 |
| 10 | The PIWI-specific insertion module helps load longer piRNAs for translational activation essential for male fertility显示文摘PIWI-clade proteins harness pi RNAs of 24–33 nt in length.Of great puzzles are how PIWI-clade proteins incorporate pi RNAs of different sizes and whether the size matters to PIWI/pi RNA function.Here we report that a PIWI-Ins module unique in PIWIclade proteins helps define the length of pi RNAs.Deletion of PIWI-Ins in Miwi shifts MIWI to load with shorter pi RNAs and causes spermiogenic failure in mice,demonstrating the functional importance of this regulatory module.Mechanistically,we show that longer pi RNAs provide additional complementarity to target m RNAs,thereby enhancing the assembly of the MIWI/e IF3f/Hu R super-complex for translational activation.Importantly,we identify a c.1108C>T(p.R370W)mutation of HIWI(human PIWIL1)in infertile men and demonstrate in Miwi knock-in mice that this genetic mutation impairs male fertility by altering the property of PIWI-Ins in selecting longer pi RNAs.These findings reveal a critical role of PIWI-Ins-ensured longer pi RNAs in fine-tuning MIWI/pi RNA targeting capacity,proven essential for spermatid development and male fertility. | Xin Wang Di-Hang Lin Yue Yan An-Hui Wang Jiaoyang Liao Qian Meng Wen-Qing Yang Heng Zuo Min-Min Hua Fengjuan Zhang Hongwen Zhu Hu Zhou Tian-Yu Huang Rui He Guangyong Li Yue-Qiu Tan Hui-Juan Shi Lan-Tao Gou Dangsheng Li Ligang Wu Yonggang Zheng Xiang-Dong Fu Jinsong Li Rujuan Liu Guo-Hui Li Mo-Fang Liu | 2023 | Science China(Life Sciences)2023，66，7： | 2 |
| 11 | Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid显示文摘Endophytic fungi can be beneficial to plant growth. However, the molecular mechanisms under-lying colonization of Acremonium spp. remain unclear. In this study, a novel endophytic Acremonium strain was isolated from the buds of Panax notoginseng and named Acremonium sp. D212. The Acremonium sp. D212 could colonize the roots of P. notoginseng, enhance the resistance of P. notoginseng to root rot disease, and promote root growth and saponin bio-synthesis in P. notoginseng. Acremonium sp. D212 could secrete indole-3-acetic acid (IAA) and jasmonic acid (JA), and inoculation with the fungus increased the endogenous levels of IAA and JA in P. noto-ginseng. Colonization of the Acremonium sp. D212 in the roots of the rice line Nipponbare was dependent on the concentration of methyl jasmonate (MeJA) (2–15μmol/L) and 1-naphthalenacetic acid (NAA) (10–20μmol/L). Moreover, the roots of the JA signaling-defective coi1-18 mutant were colonized by Acremonium sp. D212 to a lesser degree than those of the wild-type Nipponbare and miR393b-overexpressing lines, and the colonization was res-cued by MeJA but not by NAA. It suggests that the cross-talk between JA signaling and the auxin biosynthetic pathway plays a crucial role in the colonization of Acremonium sp. D212 in host plants. | Li Han Xuan Zhou Yiting Zhao Shusheng Zhu Lixia Wu Yunlu He Xiangrui Ping Xinqi Lu Wuying Huang Jie Qian Lina Zhang Xi Jiang Dan Zhu Chongyu Luo Saijie Li Qian Dong Qijing Fu Kaiyuan Deng Xin Wang Lei Wang Sheng Peng Jinsong Wu Weimin Li JiǐíFriml Youyong Zhu Xiahong He Yunlong Du | 2020 | Journal of Integrative Plant Biology2020，62，9： | 2 |
| 12 | Microstructure and Strength of Al_2O_3/Al_2O_3 Joints Bonded with ZnO—Al_2O_3—B_2O_3—SiO_2 Glass—Ceramic显示文摘ZnOeAl2O3eB2O3eSiO2(ZABS)glass powder was used as interlayer to join alumina ceramics.The effect of joining temperature on the microstructure and strength of joints was investigated.The results showed that the ZABS glass can react with alumina substrate to form a layer of ZnAl2O4at Al2O3/glass interface.Bending test exhibited that low joining temperature(1150 C)led to low joint strength due to the formation of pores in the interlayer,originated by high viscosity of the glass.High joining temperature(1250 C)also resulted in low joint strength,because of large CTE(coefficient of thermal expansion)mismatch between amorphous interlayer and alumina substrate.Therefore,only when the joining temperature was appropriate(1200 C),defect-free interface and high joint strength can be obtained.The optimum joint strength reached 285 MPa,which was the same as the base material strength. | Weiwei Zhu Jichun Chen Chuanyong Hao Jinsong Zhang | 2014 | Journal of Materials Science & Technology2014，30，9： | 2 |
| 13 | Tunable Ru-Ru2P heterostructures with charge redistribution for efficient pH-universal hydrogen evolution显示文摘Designing synergistic heterogeneous catalytic interfaces is the key to developing highly compatible pH-universal electrocatalysts for complex chemical environments.Our theoretical calculation results demonstrate that the Ru-Ru2P heterointerface can not only promote the redistribution of charges,but also reduce the d-band center,and then enhances the adsorption capacity of the key intermediate.However,in situ and facile synthesis of Ru-Ru2P heterostructures is severely limited by thermodynamic obstacles.Herein,we propose a molten salt-assisted catalytic synthesis scheme,and successfully build a series of homologous metallic Ru-Ru2P heterostructure catalysts with different molar ratios of Ru to P under atmospheric pressure and low-temperature(400C).The resultant Ru-Ru2P with rich heterostructures show the Pt-like HER performance in different pH media.Particularly,it is prominent under alkaline conditions(18 mV@10 mA cm^(2)),which outperforms the Pt catalyst(37 mV@10 mA cm^(2)).Furthermore,Ru-Ru2P heterostructures also show certain potential in the electrolysis of seawater to produce hydrogen.This work represents a significant supplement of high-efficiency pH-universal HER catalysts,and provides a new light on interface engineering in energy technology fields and beyond. | Ding Chen Ruohan Yu Ruihu Lu Zonghua Pu Pengyan Wang Jiawei Zhu Pengxia Ji Dulan Wu Jinsong Wu Yan Zhao Zongkui Kou Jun Yu Shichun Mu | 2022 | InfoMat2022，4，5： | 2 |
| 14 | A multi-functional polymer coating that is heat-resistant,hydrophobic and transparent显示文摘A multi-functional polymer film with high hydrophobicity and transparency was formed by simple casting of a polycarbonate solution onto a substrate having micro-scale roughness.The high hydrophobicity was heat-resistant,which can be retained up to 390 ℃.The polymer film may have potentially wide-ranging applications in industry and high technology. | Tianyi Zhao Dongmei Zhang Chunmei Ding Jinglun Zhou Jinsong Zhu Lei Jiang | 2014 | Particuology2014，12，6： | 2 |
| 15 | Dynamics and manipulation of ferroelectric domain walls in bismuth ferrite thin films显示文摘Ferroelectric domain walls differ from domains not only in their crystalline and discrete symmetry,but also in their electronic,magnetic,and mechanical properties.Although domain walls provide a degree of freedom to regulate the physical properties at the nanoscale;the relatively lower controllability prevents their practical applications in nano-devices.In this work;with the advantages of 3D domain configuration detection based on piezoresponse force microscopy;we find that the mobility of three types of domain walls(tail-to-tail,head-to-tail,head-to-head)in(001)BiFeO3 films varies with the applied electrical field.Under low voltages;head-to-tail domain walls are more mobile than other domain walls;while,under high voltages,tail-to-tail domain walls become rather active and possess relatively long average lengths.This is due to the high nucleation energy and relatively low growth energy for charged domain walls.Finally;we demonstrate the manipulation of domain walls through successive electric writings,resulting in well-aligned conduction paths as designed;paving the way for their application in advanced spintronic;memory and communication nano-devices. | Shuyu Xiao Yaming Jin Xiaomei Lu Sang-Wook Cheong Jiangyu Li Yang Li Fengzhen Huang Jinsong Zhu | 2020 | National Science Review2020，7，2： | 2 |
| 16 | Epitaxially Grown Ru Clusters-Nickel Nitride Heterostructure Advances Water Electrolysis Kinetics in Alkaline and Seawater Media显示文摘The epitaxial heterostructure can be rationally designed based on the in situ growth of two compatible phases with lattice similarity,in which the modulated electronic states and tuned adsorption behaviors are conducive to the enhancement of electrocatalytic activity.Herein,theoretical simulations first disclose the charge transfer trend and reinforced inherent electron conduction around the epitaxial heterointerface between Ru clusters and Ni_(3)N substrate(cRu-Ni_(3)N),thus leading to the optimized adsorption behaviors and reduced activation energy barriers.Subsequently,the defectrich nanosheets with the epitaxially grown cRu-Ni_(3)N heterointerface are successfully constructed.Impressively,by virtue of the superiority of intrinsic activity and reaction kinetics,such unique epitaxial heterostructure exhibits remarkable bifunctional catalytic activity toward electrocatalytic OER(226 mV@20 mA cm^(−2))and HER(32 mV@10 mA cm^(−2))in alkaline media.Furthermore,it also shows great application prospect in alkaline freshwater and seawater splitting,as well as solar-to-hydrogen integrated system.This work could provide beneficial enlightenment for the establishment of advanced electrocatalysts with epitaxial heterointerfaces. | Jiawei Zhu Ruihu Lu Wenjie Shi Lei Gong Ding Chen Pengyan Wang Lei Chen Jinsong Wu Shichun Mu Yan Zhao | 2023 | Energy & Environmental Materials2023，6，2： | 2 |
| 17 | Serial nuclear transfer improves the development of interspecies reconstructed giant panda (Aluropoda melanoleucd) embryos显示文摘Interspecies somatic nuclear transfer (NT) may provide a new approach for preservation of the endangered rare species. Previous interspecies cloning studies have shown that a nucleus from a quiescent somatic cell supports early development of reconstructed embryos in the ooplasm from another species. In this study, we transferred nonqui-escent somatic cells from a giant panda into the perivitelline space of the enucleated rabbit oocytes. After electrofusion (at the rate of 71.6%) and electrical activation, 4.2% of the panda-rabbit reconstructed embryos developed to blastocyst in vitro. For improving the development rate of reconstructed embryos, we used serial NT in this study, i.e. blas-tomeres from reconstructed morulae were transferred into the perivitelline space of the enucleated rabbit oocytes. The fusion rates in the groups of serial I, serial Ⅱ and serial Ⅲ were 79.5%, 84.1% and 78.0%, respectively, having no difference with that of somatic group. And the blastocyst rates in serial NT groups | LI Jinsong CHEN Dayuan HAN Zhiming ZHU Ziyu WEN Duancheng SUN Qingyuan LIU Zhonghua WANG Minkang LIAN Li DU Jun WANG Pengyan ZHANG Hemin | 2002 | Chinese Science Bulletin2002，47，6： | 2 |
| 18 | Digital Watermark-based Security Technology for Geo-spatial Graphics Data显示文摘The paper presents a set of techniques of digital watermarking by which copyright and user rights messages are hidden into geo-spatial graphics data, as well as techniques of compressing and encrypting the watermarked geo-spatial graphics data. The technology aims at tracing and resisting the illegal distribution and duplication of the geo-spatial graphics data product, so as to effectively protect the data producer’s rights as well as to facilitate the secure sharing of geo-spatial graphics data. So far in the GIS field throughout the world, few researches have been made on digital watermarking. The research is a novel exploration both in the field of security management of geo-spatial graphics data and in the applications of digital watermarking technique. An application software employing the pro- posed technology has been developed. A number of experimental tests on the 1∶500,000 digital bathymetric chart of the South China Sea and 1∶10,000 digital topographic map of Jiangsu Province have been conducted to verify the feasibility of the proposed technology. | JIA Peihong CHEN Yunzhen MA Jinsong ZHU Dakui | 2006 | Chinese Geographical Science2006，16，3： | 2 |
| 19 | High proportion strontium-doped micro-arc oxidation coatings enhance early osseointegration of titanium in osteoporosis by anti-oxidative stress pathway显示文摘The excessive accumulation of reactive oxygen species(ROS)under osteoporosis precipitates a microenvironment with high levels of oxidative stress(OS).This could significantly interfere with the bioactivity of conventional titanium implants,impeding their early osseointegration with bone.We have prepared a series of strontium(Sr)-doped titanium implants via micro-arc oxidation(MAO)to verify their efficacy and differences in osteoinduction capabilities under normal and osteoporotic(high OS levels)conditions.Apart from the chemical composition,all groups exhibited similar physicochemical properties(morphology,roughness,crystal structure,and wettability).Among the groups,the low Sr group(Sr25%)was more conducive to osteogenesis under normal conditions.In contrast,by increasing the catalase(CAT)/superoxide dismutase(SOD)activity and decreasing ROS levels,the high Sr-doped samples(Sr75% and Sr100%)were superior to Sr25% in inducing osteogenic differentiation of MC3T3-E1 cells and the M2 phenotype polarization of RAW264.7 cells,thus enhancing early osseointegration.Furthermore,the results of both in vitro cell co-culture and in vivo studies also showed that the high Sr-doped samples(especially Sr100%)had positive effects on osteoimmunomodulation under the OS microenvironment.Ultimately,the collated findings indicated that the high proportion Sr-doped MAO coatings were more favorable for osteoporosis patients in implant restorations. | Xinkun Shen Kai Fang Kendrick Hii Ru Yie Zixin Zhou Yiding Shen Shuyi Wu Yue Zhu Zhennan Deng Pingping Ma Jianfeng Ma Jinsong Liu | 2022 | Bioactive Materials2022，7，4： | 2 |
| 20 | Supersaturated polymeric micelles for oral silybin delivery: the role of the Soluplus–PVPVA complex显示文摘Increasing the degree of supersaturation of drugs and maintaining their proper stability are very important in improving the oral bioavailability of poorly soluble drugs by a supersaturated drug delivery system(SDDS). In this study, we reported a complex system of Soluplus–Copovidone(Soluplus–PVPVA)loaded with the model drug silybin(SLB) that could not only maintain the stability of a supersaturated solution but also effectively promote oral absorption. The antiprecipitation effect of the polymers on SLB was observed using the solvent-shift method. In addition, the effects of the polymers on absorption were detected by cellular uptake and transport experiments. The mechanisms by which the Soluplus–PVPVA complex promotes oral absorption were explored by dynamic light scattering, transmission electron microscopy, fluorescence spectra and isothermal titration calorimetry analyses. Furthermore, a pharmacokinetic study in rats was used to demonstrate the advantages of the Soluplus–PVPVA complex. The results showed that Soluplus and PVPVA spontaneously formed complexes in aqueous solution via the adsorption of PVPVA on the hydrophilichydrophobic interface of the Soluplus micelle, and the Soluplus–PVPVA complex significantly increased the absorption of SLB. In conclusion, the Soluplus–PVPVA complex is a potential SDDS for improving the bioavailability of hydrophobic drugs. | Chunliu Zhu Shuang Gong Jinsong Ding Miaorong Yu Ejaj Ahmad Yi Feng Yong Gan | 2019 | Acta Pharmaceutica Sinica B2019，9，1： | 2 |
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