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| 1 | Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer(IVF-ET)显示文摘This is a retrospective,observational study to evaluate the effect of maternal age on the outcomes of in vitro fertilization and embryo transfer(IVF-ET).11830 IVF-ET cycles from 10268 women were included.Four groups of different maternal age periods were compared.The groups were 21 30 years old group(4549 cycles),31-35 years old group(4424 cycles),36-40 years old group(2429 cycles),and over 40 years old group(428 cycles).The mean starting dose of Gn and mean total dose of Gn in each cycle were significantly higher(P<0.01),while the mean retrieved oocyte number was significantly lower(P<0.01) in groups of higher maternal age period than those in each of the lower groups.The biochemical pregnancy rate and the clinical pregnancy rate were significantly lower(P<0.01),while the miscarriage rate was significantly higher(P<0.01) in groups of higher maternal age period than those in the lower groups.No difference was found in two-pronuclear zygotes(2PN) rate and good quality embryo rate among different groups.Birth defect rate was also comparable in the born babies in different groups.In the group with patients' age over 40 years old,the pregnancy rate was 26.87%,the clinical pregnancy rate was 19.39%,while the miscarriage rate after clinical pregnancy was 36.14%.To draw the conclusion,patients with higher maternal age had worse IVF outcomes.In women of fertile age,patients between 20 and 30 years old have the best IVF outcomes.Patients over 40 years old have poor IVF outcome and high miscarriage rate,which suggested the necessity of preimplantation genetic screening(PGS). | YAN JunHao WU KeLiang TANG Rong DING LingLing CHEN Zi-Jiang | 2012 | Science China(Life Sciences)2012，55，8： | 23 |
| 2 | Arctic Sea Ice and Eurasian Climate:A Review显示文摘The Arctic plays a fundamental role in the climate system and has shown significant climate change in recent decades,including the Arctic warming and decline of Arctic sea-ice extent and thickness. In contrast to the Arctic warming and reduction of Arctic sea ice, Europe, East Asia and North America have experienced anomalously cold conditions, with record snowfall during recent years. In this paper, we review current understanding of the sea-ice impacts on the Eurasian climate.Paleo, observational and modelling studies are covered to summarize several major themes, including: the variability of Arctic sea ice and its controls; the likely causes and apparent impacts of the Arctic sea-ice decline during the satellite era,as well as past and projected future impacts and trends; the links and feedback mechanisms between the Arctic sea ice and the Arctic Oscillation/North Atlantic Oscillation, the recent Eurasian cooling, winter atmospheric circulation, summer precipitation in East Asia, spring snowfall over Eurasia, East Asian winter monsoon, and midlatitude extreme weather; and the remote climate response(e.g., atmospheric circulation, air temperature) to changes in Arctic sea ice. We conclude with a brief summary and suggestions for future research. | GAO Yongqi SUN Jianqi LI Fei HE Shengping Stein SANDVEN YAN Qing ZHANG Zhongshi Katja LOHMANN Noel KEENLYSIDE Tore FUREVIK SUO Lingling | 2015 | Advances in Atmospheric Sciences2015，32，1： | 21 |
| 3 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 4 | Rice Functional Genomics Research: Past Decade and Future显示文摘瑞斯(Oryza sativa ) 是为世界范围的超过 35 亿个人的主要主食食物庄稼。在米饭理解复杂农学的特点的规章的机制为全球食物安全是批评的。瑞斯也是为单子叶植物的 genomics 研究的模型植物。由于功能的 genomic 技术的快速的发展,控制重要农学的特点的超过 2000 基因被克隆,并且他们的分子的生物机制也是部分描绘了。这里,我们简短在米饭考察进展功能的 genomics 研究在过去的 10 年期间包括功能的 genomics 平台的一篇摘要,基因 ? 并且调整重要农学的特点的分子的规章的网络,并且 ? 为基因鉴定的最新发达的工具。在功能的 genomics 研究做的这些成就将极大地便于发展 ? 绿超级米饭。我们也讨论未来挑战和米饭的前景功能的 genomics 研究。 | Yan Li Jinghua Xiaot Lingling Chen Xuehui Huang Zhukuan Cheng Bin Han Qifa Zhang Changyin Wu | 2018 | Molecular Plant2018，11，3： | 14 |
| 5 | Characteristics of secondary inorganic aerosol and sulfate species in size-fractionated aerosol particles in Shanghai显示文摘Sulfate, nitrate and ammonium(SNA) are the dominant species in secondary inorganic aerosol, and are considered an important factor in regional haze formation. Size-fractionated aerosol particles for a whole year were collected to study the size distribution of SNA as well as their chemical species in Shanghai. SNA mainly accumulated in fine particles and the highest average ratio of SNA to particulate matter(PM) was observed to be 47% in the fine size fraction(0.49–0.95 μm). Higher sulfur oxidation ratio and nitrogen oxidation ratio values were observed in PM of fine size less than 0.95 μm. Ion balance calculations indicated that more secondary sulfate and nitrate would be generated in PM of fine size(0.49–0.95 μm). Sulfur K-edge X-ray absorption near-edge structure(XANES) spectra of typical samples were analyzed. Results revealed that sulfur mainly existed as sulfate with a proportion(atomic basis) more than 73% in all size of PM and even higher at 90% in fine particles. Sulfate mainly existed as(NH4)2SO4 and gypsum in PM of Shanghai. Compared to non-haze days, a dramatic increase of(NH4)2SO4 content was found in fine particles on haze days only, which suggested the promoting impact of(NH4)2SO4 on haze formation. According to the result of air mass backward trajectory analysis, more(NH4)2SO4 would be generated during the periods of air mass stagnation. Based on XANES, analysis of sulfate species in size-fractionated aerosol particles can be an effective way to evaluate the impact of sulfate aerosols on regional haze formation. | Shilei Long Jianrong Zeng Yan Li Liangman Bao Lingling Cao Ke Liu Liang Xu Jun Lin Wei Liu Guanghua Wang Jian Yao Chenyan Ma Yidong Zhao | 2014 | Journal of Environmental Sciences2014，26，5： | 10 |
| 6 | Glycine attenuates myocardial ischemia-reperfusion injury by inhibiting myocardial apoptosis in rats显示文摘Glycine is a well-documented cytoprotective agent.However,whether it has a protective effect against myocar-dial ischemia-reperfusion injury in vivo is still unknown.By using an open-chest anesthetized rat model,we found that glycine reduced the infarct size by 21% in ischemia-reperfusion injury rats compared with that in the vehicle-treated MI/R rats.The left ventricular ejection fraction and fractional shortening were increased by 19.11% and 30.98%,respectively,in glycine-treated rats.The plasma creatine kinase levels in ischemia-reperfusion injury rats decreased following glycine treatment.Importantly,administration of glycine significantly inhibited apoptosis in post-ischemia-reperfusion myocardium,which was accompanied by suppression of phosphorylated p38 mitogen-activated protein kinase and c-Jun NH2-terminal kinase,as well as the Fas ligand.These results suggest that gly-cine attenuates myocardial ischemia-reperfusion injury in vivo by inhibiting cardiomyocytes apoptosis. | Xiaozheng Zhong Xiaoyu Li Lingling Qiana Yiming Xu Yan Lu Jing Zhang Nan Li Xudong Zhu Jingjing Ben Qing Yang Qi Chen | 2012 | The Journal of Biomedical Research2012，26，5： | 10 |
| 7 | WIP1 Phosphatase Plays a Critical Neuroprotective Role in Brain Injury Induced by High-Altitude Hypoxic Inflammation显示文摘The hypobaric hypoxic environment in highaltitude areas often aggravates the severity of inflammation and induces brain injury as a consequence. However, the critical genes regulating this process remain largely unknown. The phosphatase wild-type p53-induced phosphatase 1(WIP1) plays important roles in various physiological and pathological processes, including the regulation of inflammation in normoxia, but its functions in hypoxic inflammation-induced brain injury remain unclear.Here, we established a mouse model of this type of injury and found that WIP1 deficiency augmented the release of inflammatory cytokines in the peripheral circulation and brain tissue, increased the numbers of activated microglia/macrophages in the brain, aggravated cerebral histological lesions, and exacerbated the impairment of motor and cognitive abilities. Collectively, these results provide the first in vivo evidence that WIP1 is a critical neuroprotector against hypoxic inflammation-induced brain injury. | Dahu Li Lijun Zhang Xin Huang Lili Liu Yunling He Lun Xu Yiyao Zhang Tong Zhao Liying Wu Yongqi Zhao Kuiwu Wu Yan Wu Ming Fan Lingling Zhu | 2017 | Neuroscience Bulletin2017，33，3： | 9 |
| 8 | Sequential fate-switches in stem-like cells drive the tumorigenic trajectory from human neural stem cells to malignant glioma显示文摘Glioblastoma(GBM)is an incurable and highly heterogeneous brain tumor,originating from human neural stem/progenitor cells(hNSCs/hNPCs)years ahead of diagnosis.Despite extensive efforts to characterize hNSCs and end-stage GBM at bulk and single-cell levels,the de novo gliomagenic path from hNSCs is largely unknown due to technical difficulties in early-stage sampling and preclinical modeling.Here,we established two highly penetrant hNSC-derived malignant glioma models,which resemble the histopathology and transcriptional heterogeneity of human GBM.Integrating time-series analyses of whole-exome sequencing,bulk and single-cell RNA-seq,we reconstructed gliomagenic trajectories,and identified a persistent NSC-like population at all stages of tumorigenesis.Through trajectory analyses and lineage tracing,we showed that tumor progression is primarily driven by multi-step transcriptional reprogramming and fate-switches in the NSC-like cells,which sequentially generate malignant heterogeneity and induce tumor phenotype transitions.We further uncovered stage-specific oncogenic cascades,and among the candidate genes we functionally validated C1QL1 as a new glioma-promoting factor.Importantly,the neurogenic-to-gliogenic switch in NSC-like cells marks an early stage characterized by a burst of oncogenic alterations,during which transient AP-1 inhibition is sufficient to inhibit gliomagenesis.Together,our results reveal previously undercharacterized molecular dynamics and fate choices driving de novo gliomagenesis from hNSCs,and provide a blueprint for potential early-stage treatment/diagnosis for GBM. | Xiaofei Wang Ran Zhou Yanzhen Xiong Lingling Zhou Xiang Yan Manli Wang Fan Li Chuanxing Xie Yiming Zhang Zongyao Huang Chaoqiong Ding Kaidou Shi Weida Li Yu Liu Zhongwei Cao Zhen-Ning Zhang Shengtao Zhou Chong Chen Yan Zhang Lu Chen Yuan Wang | 2021 | Cell Research2021，31，6： | 8 |
| 9 | The role of transcriptional factor D-site-binding protein in circadian CCL2 gene expression in anti-Thy1 nephritis显示文摘Mesangial proliferative glomerulonephritis(MsPGN)is an inflammatory disease,but both the nature of disease progression and its regulation remain unclear.In the present study,we monitored the course of anti-Thy1 nephritis from days 1 to 5 and established gene expression profiles at each time point using microarrays to explore the development of inflammation.According to the gene expression profiles,macrophage infiltration(triggered by CCL2 activation)was evident on day 1 and enhanced inflammation over the next few days.We screened for genes with expression levels similar to CCL2 and found that the upregulation of the circadian gene albumin D-site-binding protein(DBP)was involved in CCL2 activation in mesangial cells.More importantly,CCL2 expression showed oscillatory changes similar to DBP,and DBP induced peak CCL2 expression at 16:00 a clock on day 1 in the anti-Thy1 nephritis model.We knocked down DBP through transfection with a small interfering RNA(siRNA)and used RNA sequencing to identify the DBP-regulated TNF-α-CCL2 pathway.We performed chromatin immunoprecipitation sequencing(ChIP-Seq)and the dual luciferase assay to show that DBP bound to the TRIM55 promoter,regulating gene expression and in turn controlling the TNF-α-CCL2 pathway.In conclusion,DBP-regulated circadian CCL2 expression by the TRIM55-TNF pathway in injured mesangial cells at an early stage,which promoted macrophage recruitment and in turn triggered infiltration and inflammation in a model of anti-Thy1 nephritis. | Yang Lu Yan Mei Lei Chen Lingling Wu Xu Wang Yingjie Zhang Bo Fu Xizhao Chen Yuansheng Xie Guangyan Cai Xueyuan Bai Qinggang Li Xiangmei Chen | 2019 | Cellular & Molecular Immunology2019，16，9： | 7 |
| 10 | Activated cytotoxic lymphocytes promote tumor progression by increasing the ability of 3LL tumor cells to mediate MDSC chemoattraction via Fas signaling显示文摘Fas/FasL 系统播送细胞内部的 apoptotic 发信号,导致房间 apoptosis。然而,船边交货发信号也除了导致肿瘤房间 apoptosis 施加 non-apoptotic 功能。例如,船边交货发信号导致肺癌症肿瘤房间生产前列腺素 E2 (PGE2 ) 和成员导出 myeloid 的 suppressor 房间(MDSC ) 。激活的细胞毒素的 T 淋巴细胞(CTL ) 导致并且表示 FasL 在房间仍然是的 apoptosis 抵抗的肿瘤上在 CTL 开始的船边交货激活的 FasL,而是效果的高水平大部分不清楚。我们从 OT-1 老鼠净化了激活的 CD8 + T 房间,在肿瘤房间逃跑上评估了船边交货激活的规章的效果并且调查了相关机制。我们发现 CTL 导致了肿瘤细胞分泌 PGE2 和增加肿瘤经由船边交货发信号的 MDSC 的调停房间的 chemoattraction,它对肿瘤生长有利。我们的结果显示 CTL 可以参予肿瘤免疫者避免过程。就我们的知识而言,这是 CTL 在肿瘤逃跑由起一个作用的新奇机制。我们的调查结果含有经由对肿瘤的否定有免疫力的回答的减小提高 antitumor 免疫者反应的策略通过船边交货发信号由 CTL 支持了。 | Fei Yang Yinxiang Wei Zhijian Cai Lei Yu Lingling Jiang Chengyan Zhang Huanmiao Yan Qingqing Wang Xuetao Cao Tingbo Liang Jianli Wang | 2015 | Cellular & Molecular Immunology2015，12，1： | 6 |
| 11 | Indian hedgehog mutations causing brachydactyly type A1 impair Hedgehog signal transduction at multiple levels显示文摘Brachydactyly 打 A1 (BDA1 ) ,第一在人记录了孟德尔的正染色体的主导的混乱,被弄短或中间的指骨的缺席描绘。在印度刺猬(IHH ) 基因的异质接合的错误感觉变化作为 BDA1 的一个原因被识别了;然而,这些变化的生物化学的后果是不清楚的。在这份报纸,我们在印度刺猬(IhhN ) 的 N 终端碎片分析了三个 BDA1 变化(E95K, D100E,和 E131K ) 。结构的分析证明 E95K 变化在一条钙绑定沟把一个否定地控告的区域改变到一个断然控告的区域,并且 D100E 变化改变本地第三级的结构。而且,我们证明 E95K 和 D100E 变化导致了一温度敏感并且 IhhN 的钙依赖者不稳定性,它可能经由 lysosome 贡献变异的蛋白质的提高的细胞内部的降级。尤其是,所有三个变化影响了对受体 Patched1 (PTC1 ) 有约束力的 Hh,减少它的能力导致细胞的区别。我们建议这些是引起 BDA1 的变化的普通特征,影响 Hh 第三级的结构,细胞内部的命运,到受体 / 搭挡的绑定,和到细胞外的部件的绑定。这些特征的联合改变发信号的能力和范围,但是影响是可能的是变量和变化依赖者。在发信号的范围的潜在的变化被和 heparan 硫酸盐的一个提高的相互作用与 E95K 变化,然而并非 E131K 变化为 IHH 描绘。一起拿,我们的结果建议这些 IHH 变化影响在多重层次,引起的反常骨头发展和反常的位形成发信号的 Hh。 | Gang Ma Jiang Yu Yue Xiao Danny Chan Bo Gao Jianxin Hu Yongxing He Shengzhen Guo Jian Zhou Lingling Zhang Linghan Gao Wenjuan Zhang Yan Kang Kathryn SE Cheah Guoyin Feng Xizhi Guo Yujiong Wang Cong-zhao Zhou Lin He | 2011 | Cell Research2011，21，9： | 6 |
| 12 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 13 | Progress in the development of techniques based on light scattering for single nanoparticle detection显示文摘Nanoparticles have recently attracted extensive attention in view of their great potential in biomedicine and bioanalytical ap- plications. Single particle detection via light scattering offers a simple and efficient approach for the size, size distribution, and concentration analysis of nanoparticles. In particular, intrinsic heterogeneity or rare events masked by ensemble averaging can be revealed. However, the sixth power dependence of Rayleigh scattering on particle size makes it very challenging to detect individual nanoparticles of small sizes. This article is intended to provide an overview of recent progress in the development of techniques based on light scattering for the detection of single nanoparticles. | ZHU ShaoBin, WANG Shuo, YANG LingLing, HUANG TianXun & YAN XiaoMei Department of Chemical Biology, College of Chemistry and Chemical Engineering Key Laboratory for Chemical Biology of Fujian Province Key Laboratory of Analytical Science, Xiamen University, Xiamen 361005, China | 2011 | Science China Chemistry2011，54，8： | 5 |
| 14 | Methionine deficiency and its hydroxy analogue influence chicken intestinal 3-dimensional organoid development显示文摘Methionine and its hydroxy analogue(MHA)have been shown to benefit mouse intestinal regeneration.The intestinal organoid is a good model that directly reflects the impact of certain nutrients or chemicals on intestinal development.Here,we aimed to establish a chicken intestinal organoid culture method first and then use the model to explore the influence of methionine deficiency and MHA on intestinal organoid development.The results showed that 125-mm cell strainer exhibited the highest efficiency for chicken embryo crypt harvesting.We found that transforming growth factor-b inhibitor(A8301)supplementation promoted enterocyte differentiation at the expense of the proliferation of intestinal stem cells(ISC).The mitogen-activated protein kinase p38 inhibitor(SB202190)promoted intestinal organoid formation and enterocyte differentiation but suppressed the differentiation of enteroendocrine cells,goblet cells and Paneth cells.However,the suppression of enteroendocrine cell and Paneth cell differentiation by SB202190 was alleviated at the presence of A8301.The glycogen synthase kinase 3 inhibitor(CHIR99021),valproic acid(VPA)alone and their combination promoted chicken intestinal organoid formation and enterocyte differentiation at the expense of the expression of Paneth cells and goblet cells.Chicken serum significantly improved organoid formation,especially in the presence of A8301,SB202190,CHIR99021,and VPA,but inhibited the differentiation of Paneth cells and enteroendocrine cells.Chicken serum at a concentration of 0.25%meets the requirement of chicken intestinal organoid development,and the beneficial effect of chicken serum on chicken intestinal organoid culture could not be replaced by fetal bovine serum and insulin-like growth factor-1.Moreover,commercial mouse organoid culture medium supplemented with A8301,SB202190,CHIR99021,VPA,and chicken serum promotes chicken organoid budding.Based on the chicken intestinal organoid model,we found that methionine deficiency mimicked by cycloleucine suppressed organoid formation and organoid size,and this effect was reinforced with increased cycloleucine concentrations.Methionine hydroxy analogue promoted regeneration of ISC but decreased cell differentiation compared with the results obtained with L-methionine.In conclusion,our results provide a potentially excellent guideline for chicken intestinal organoid culture and insights into methionine function in crypt development. | Youli Wang Qihang Hou Yuqin Wu Yanwei Xu Yan Liu Jing Chen Lingling Xu Yuming Guo Shuai Gao Jianmin Yuan | 2022 | Animal Nutrition2022，，1： | 4 |
| 15 | Loss of Wip1 aggravates brain injury after ischaemia/reperfusion by overactivating microglia显示文摘Background and purpose The inflammatory response mediated by microglia/macrophages is closely related to cerebral ischaemia/reperfusion injury.Wild-type p53-induced protein phosphatase 1(Wip1),a serine/threonine phosphatase,is expressed in various tissues.A growing number of reports have suggested that Wip1 is a negative regulator of inflammation in peripheral tissue;however,its role in the central nervous system(CNS)remains unclear.This study aimed to clarify whether Wip1 can inhibit CNS inflammation by regulating microglia/macrophage functions after ischaemic injury.Methods A model of middle cerebral artery occlusion and reperfusion was established in mice.CNS inflammation was simulated by lipopolysaccharide treatment of primary microglia.Laser speckle imaging was used to monitor regional cerebral blood flow.Behavioural outcomes were assessed with a TreadScan gait analysis system.TTC staining was used to evaluate the infarct volume,and western blotting and immunofluorescence staining were applied to detect the phenotypical transformation of microglia.ELISA was performed to detect the levels of inflammatory factors.Results Wip1 expression was increased after ischaemia/reperfusion.Wip1-knockout(KO)mice displayed more severe brain injury than wild-type mice,as indicated by aggravated motor dysfunction,greater brain infarct volumes and higher expression of inflammatory cytokines(interleukin-6 and tumour necrosis factor alpha)in the brain.We also found that Wip1 depletion increased microglial/macrophage activation in both in vitro and in vivo models,which all showed activation of microglia/macrophages.Lentivirus-Ppm1d reversed the injury induced by Wip1-KO.Conclusions Our results suggest that Wip1 may inhibit neuroinflammation by inhibiting microglial/macrophage activation after brain ischaemia/reperfusion injury. | Feng Yan Xiang Cheng Ming Zhao Shenghui Gong Ying Han Liping Ding Di Wu Yumin Luo Wei Zuo Lingling Zhu Ming Fan Xunming Ji | 2021 | Stroke & Vascular Neurology2021，6，3： | 4 |
| 16 | CD146:a potential therapeutic target For systemic sclerosis显示文摘Dear Editor, Systemic sclerosis (scleroderma,SSc)is a chronic disease of connective tissues,and is clinically characterized by persistent fibrosis in the skin as well as in a variety of organs (Katsumoto et al.,2011).The pathogenesis of SSc is com- plex and involves vasculopathy,autoimmunity and fibrosis. The hallmarks of late-stage SSc are the excessive secretion and accumulation of extracellular matrix (ECM)by aberrantly activated fibroblasts (myofibroblasts)in the skin and internal organs (Bhattacharyya et al.,2012). | Lingling Zhang Yongting Luo Xiao Teng Zhenzhen Wu Mengtao Li Dong Xu Qian Wang Fei Wang Jing Feng Xiaofeng Zeng Xiyun Yan | 2018 | Protein & Cell2018，9，12： | 4 |
| 17 | Electroluminescence efficiency in bilayer organic light-emitting devices with LiF/Al cathode显示文摘An analytical model to calculate electroluminescence (EL) efficiency of bilayer organic light-emitting devices, considering the influence of introducing LiF insulating buffer layer at metal/organic interface on the barrier height for electron injection, was presented. The relations of EL efficiency versus the applied voltage and injection barrier or internal interfacial barrier or the thickness of organic layer were discussed. The results indicate that: (1) when δ e/δ h < 2, metal/organic (M/O) interface is ohmic contact; when δ e/δ h > 2, M/O becomes contact limited; and when δ e/δ h = 2 (Φ h ~ 0.2 eV, Φ e ~ 0.3 eV), there is a transition from ohmic contact to contact limited; (2) η EL decreases with the increase of δ′e / δ′h; however, when δ′e / δ′h > 2.5 (H ′h~ 0.2 eV, H ′e~ 0.4 eV), the changes of η EL are very small, which shows that η EL is dominated by the carrier’s injection; (3) when increasing Lh/L, η R has a descending trend at low voltage and a rising one at higher voltage. For a given Lh/L, η EL first increases and then decreases with the increasing applied voltage, and as Lh/L further increases, the variation tendency of η EL is more obvious. These conclusions are in agreement with the reported theoretic and experimental results. | LI HongJian ZHU RuHui YAN LingLing ZHANG HaiYan | 2007 | Science China(Physics,Mechanics & Astronomy)2007，50，1： | 4 |
| 18 | Laser textured dimple-patterns to govern the surface wettability of superhydrophobic aluminum plates显示文摘A laser texturing technique herein can endow bare aluminum alloy surface with regular dimple-pattern array and thus generates a case hardening.After STA treatment,these laser-textured samples become superhydrophobic.The surface wettability of the laser-textured samples can be regulated by controlling the dimple-pattern dimensions during the laser processing.It is noteworthy that a fluorescence method is utilized to record the zones on the superhydrophobic surface penetrated by small enough water molecules.Compared with a general method of the Cassie-Baxter theoretical calculation,this fluorescence method intuitively exhibits the air trapping ability of the superhydrophobic surface.Furthermore,the laser-textured superhydrophobic samples have a notable hysteresis phenomenon at the initial period of UMT friction because the air cushion trapped within superhydrophobic samples have strong repellency against water droplets on the hydrophilic steel ball.Additionally,such samples display strong mechanical stability in comparison with bare aluminum alloy because of the presence of case hardening on the surface of the laser patterns.The research results above provide a valuable reference for designing a surface with different wettability,which may inspire practical applications in the fields of fluid transport,droplet manipulation,water harvesting and microfluidic devices. | Wei Tong Lingling Cui Rongxian Qiu Chengqi Yan Yuntong Liu Nan Wang Dangsheng Xiong | 2021 | Journal of Materials Science & Technology2021，，30： | 3 |
| 19 | Application of microcalorimetry of Escherichia coli growth and discrimin antanalysis to the quality assessment of a Chinese herbal injection(Yinzhihuang)显示文摘This paper describes a novel approach to the quality control of a Chinese herbal injection based on microca lorimetric determination of its effect on the biothermal active fingerprint(BTAF)of Escherichia coli(E.coli)growth.Yinzhihuang Injection(YZHI)was selected for this proof of concept study.Reference samples of Y ZHI were collected and compared with test(stressed)samples prepared under different stress conditions.The BTAF of E.coli growth was found to be affected by YZHI and the changes were analyzed on the basis of eleven biothermokinetic parameters.Similarity and multivariate statistical analysis were used to investi-gate the differences between reference and test samples and discriminant analysis was used to delineate the altered samples.Reference samples were found to have coincident BTAFs with similarity index>0.99.Stressed samples showed differences in the BTAF which increased in line with decreased quality.Discriminant formulae were developed based on a sensitivity parameter which could identify all altered samples.In conclusion,BTAF can be used to assess the quality of YZHI both qualitatively and quantitatively and has the potential to provide a sensitive method for quality control of Chinese herbal injections. | Yongshen Ren Ping Zhang Dan Yan Yan Yan Longhu Chen Lingling Qiu Zhinan Mei Xiaohe Xiao | 2012 | Acta Pharmaceutica Sinica B2012，2，3： | 3 |
| 20 | Preparation, microstructure and degradation performance of biomedical magnesium alloy fine wires显示文摘With the development of new biodegradable Mg alloy implant devices, the potential applications of biomedical Mg alloy fine wires are realized and explored gradually. In this study, we prepared three kinds of Mg alloy fine wires containing 4 wt% RE(Gd/Y/Nd) and 0.4 wt% Zn with the diameter less than 0.4 μm through casting, hot extruding and multi-pass cold drawing combined with intermediated annealing process. Their microstructures, mechanical and degradation properties were investigated. In comparison with the corresponding as-extruded alloy, the final fine wire has significantly refined grain with an average size of 3–4 μm, and meanwhile shows higher yield strength but lower ductility at room temperature. The degradation tests results and surface morphologies observations indicate that Mg–4Gd–0.4Zn and Mg–4Nd–0.4Zn fine wires have similar good corrosion resistance and the uniform corrosion behavior in SBF solution. By contrast, Mg–4Y–0.4Zn fine wire shows a poor corrosion resistance and the pitting corrosion behavior. | Jing Bai Lingling Yin Ye Lu Yiwei Gan Feng Xue Chenglin Chu Jingli Yan Kai Yan Xiaofeng Wan Zhejun Tang | 2014 | Progress in Natural Science:Materials International2014，24，5： | 3 |
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