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| 1 | Elevation of tumor necrosis factor-α,interleukin-1β and interleukin-6 levels in aortic intima of Chinese Guizhou minipigs with streptozotocin-induced diabetes显示文摘有调停毒素的胰腺的损坏的背景大动物模型关于糖尿病 mellitus 和心血管的糖尿病的复杂并发症在研究广泛地被使用了。试图证实有 streptozotocin ( STZ )的中国贵州 minipig 模型导致了糖尿病并且描绘动物的现在的学习由在大动脉的墙中分析煽动性的 cytokine 层次当模特儿,例如肿瘤坏死因素( TNF ), interleukin-1 ( IL-1 )和 interleukin-6 ( IL-6 ) .Methods 22 男中国贵州 minipigs (变老, 4~6 个月;重量, 20 kg 到 30 kg ) 被划分成导致 STZ 的糖尿病的组(n=12 ) 和控制组(n=10 ) 。STZ (125 mg/kg ) 被管理导致多糖症,以后,胰岛素被用来在 10 mmol/L 下面控制 fasting 血葡萄糖层次。口头的葡萄糖忍耐测试(OGTT ) 以前被执行并且在 STZ 管理和丙氨酸 transaminase,天门冬素 transaminase,白朊,血脲氮, creatinine,类脂化合物和白色的浆液集中以后的一个月血房间计数以前并且六个月以后被测量。在六个月和胰为小岛房间 immunohistochemically 被检验以后,在两个组的动物是 euthanized。糖尿病的 minipigs 和控制的大动脉的 intima 在由西方的污点中等的调节的组织为 TNF- 水平被分析。在大动脉的 intima 的 TNF- , IL-1 和 IL-6 mRNA 层次是由反向的抄写和聚合酶链反应(RT-PCR ).Results 的 assayed 在浆液葡萄糖层次的重要举起被观察在 STZ 正式就职(P0.001 ) 和显著地增加的 OGTT 价值以后的一个月和六个月被注意,与基线数据相比。正常的胰有许多不规则的大小的小岛和小岛房间的小簇,当在糖尿病的 minipigs 的胰小岛房间几乎消失了时。没有统计差别以前在生物化学的考试的浆液集中被通知并且在 STZ 以后的六个月感应。西方的污点证明在 aotic intima 的戏剧性地增加的TNF-水平调节了TNF-, IL-1 和 IL-6 的中等、重要的举起 mRNA 层次被现在的学习建立了的 RT-PCR.Conclusions 揭示有 导致STZ 的 diabetes.Inflammatory cytokines 的中国贵州 minipig 模型(TNF-, IL-1 和 IL-6 )显著地在糖尿病的 minipigs 的大动脉的 intima 提高了。 | LU Lin ZHANG Qi PU Li-jin XU Xue-wei ZHANG Rui-yan ZHANG Jian-sheng HU Jian YANG Zheng-kun LU An-kang DING Feng-hua SHEN Jie CHEN Qiu-jin LOU Sheng FANG Dan-hong SHEN Wei-feng | 2007 | Chinese Medical Journal2007，，6： | 43 |
| 2 | Mesenchymal stem cells over-expressing hepatocyte growth factor improve small-for-size liver grafts regeneration显示文摘 | Yu, Y. Yao, A. H. Chen, N. Pu, L. Y. Fan, Y. Lv, L. Sun, B. C. Li, G. Q. Wang, X. H. | 2007 | 南京医科大学学报(自然科学版)2007，27，10： | 45 |
| 3 | The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis显示文摘Artemisia annua,通常已知的同样香甜的苦恼或 Qinghao,是到中国的一个灌木土著人并且长被用于药用的目的。A。annua 现在作为有势力抗疟药混合物的唯一的生来的来源全球性被栽培, artemisinin。这里,我们报导 A 的 1.74-gigabase 染色体的一个高质量的草稿集会。annua,高度异质接合,富于重复定序,并且包含 63 ? 226 编码蛋白质的基因,在定序的植物种类之中的最大的数字之一。我们发现了那,作为在 Asteraceae 的一些定序的染色体之一, A。annua 染色体包含对这大被子植物 clade 特定的很多基因。尤其是,扩大和编码涉及萜烯生合成的酶的基因的功能的多样化与 artemisinin biosynthetic 小径的进化一致。我们进一步由介绍那 A 的 transcriptome 揭示了。annua 发展了复杂 transcriptional 规章的网络位于 \O 下面 artemisinin 生合成。把转基因的 A 基于我们产生了的全面 genomic 和 transcriptomic 分析。artemisinin 高级的生产的 annua 线,它现在为大规模生产准备好了并且将从而帮助遇见增加 artemisinin 的全球需求的挑战。 | Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang | 2018 | Molecular Plant2018，11，6： | 47 |
| 4 | Exotic origin of the Chinese continental shelf: new insights into the tectonic evolution of the western Pacific and eastern China since the Mesozoic显示文摘The effect of paleo-Pacific subduction on the geological evolution of the western Pacific and continental China is likely complex. Nevertheless, our analysis of the distribution of Mesozoic granitoids in the eastern continental China in space and time has led us to an interesting conclusion: The basement of the continental shelf beneath East and South China Seas may actually be of exotic origin geologically unrelated to the continental lithosphere of eastern China. By accepting the notion that the Jurassic–Cretaceous granitoids in the region are genetically associated with western Pacific subduction and the concept that subduction may cease to continue only if the trench is being jammed, then the termination of the granitoidmagmatism throughout the vast region at *88 ± 2 Ma manifests the likelihood of ‘‘sudden'', or shortly beforehand(*100 Ma), trench jam of the Mesozoic western Pacific subduction. Trench jam happens if the incoming‘‘plate'' or portion of the plate contains a sizeable mass that is too buoyant to subduct. The best candidate for such a buoyant and unsubductable mass is either an oceanic plateau or a micro-continent. We hypothesize that the basement of the Chinese continental shelf represents such an exotic, buoyant and unsubductable mass, rather than seaward extension of the continental lithosphere of eastern China. The locus of the jammed trench(i.e., the suture) is predictably located on the shelf in the vicinity of, and parallel to, the arc-curved coastal line of the southeast continental China. It is not straightforward to locate the locus in the northern section of the East China Sea shelf because of the more recent(\20 Ma) tectonic re-organization associated with the opening of the Sea of Japan. We predict that the trench jam at *100 Ma led to the re-orientation of the Pacific plate motion in the course of NNW direction as inferred from the age-progressive Emperor Seamount Chain of Hawaiian hotspot origin(its oldest unsubdued Meiji and Detroit seamounts are *82 Ma),making the boundary between the Pacific plate and the newly accreted plate of eastern Asia a transform fault at the location east of the continental shelf of exotic origin. This explains the apparent *40 Myr magmatic gap from *88to *50 Ma prior to present-day western Pacific subduction initiation. We propose that basement penetration drilling on well-chosen sites is needed to test the hypothesis in order to reveal the true nature of the Chinese continental shelf basement. This testing becomes critical and cannot longer be neglected in order to genuinely understand the tectonic evolution of the western Pacific and its effect on the geology of eastern China since the Mesozoic, includingthe cratonic lithosphere thinning, related magmatism/mineralization, and the mechanism of the subsequent South China Sea opening, while also offering novel perspectives on aspects of the plate tectonics theory. We also suggest the importance of future plate tectonic reconstruction of the western Pacific to consider the nature and histories of the Chinese continental shelf of exotic origin as well as the probable transform plate boundary from *100 to*50 Ma. Effort is needed to reveal the true nature and origin of the *88 ± 2 Ma granitic gneisses in Taiwan and the 110–88 Ma granitoids on the Hainan Island. | Yaoling Niu Yi Liu Qiqi Xue Fengli Shao Shuo Chen Meng Duan Pengyuan Guo Hongmei Gong Yan Hu Zhenxing Hu Juanjuan Kong Jiyong Li Jinju Liu Pu Sun Wenli Sun Lei Ye Yuanyuan Xiao Yu Zhang | 2015 | Science Bulletin2015，60，18： | 32 |
| 5 | Analysis of Pathological data of renal biopsy at one single center in China from 1987 to 2012显示文摘 | Zhang Xueguang Liu Shuwen Tang Li Wu Jie Chen Pu Yin Zhong Li Minxia Xie Yuansheng Cai Guangyan Wei Ribao Qiu Qiang Wang Yuanda Shi Suozhu Chen Xiangmei | 2014 | Chinese Medical Journal2014，，9： | 30 |
| 6 | Epigenetic silencing of microRNA-149 in cancer-associated fibroblasts mediates prostaglandin E2/interleukin-6 signaling in the tumor microenvironment显示文摘 | Pu Li Jing-Xuan Shan Xue-Hua Chen Di Zhang Li-Ping Su Xiu-Ying Huang Bei-Qin Yu Qiao-Ming Zhi Cheng-Long Li Ya-Qing Wang Sara Tomei Qu Cai Jun Ji Jian-Fang Li Lotfi Chouchane Ying-Yan Yu Fang-Zhen Sun Zhi-Heng Xu Bing-Ya Liu Zheng-Gang Zhu | 2015 | Cell Research2015，25，5： | 24 |
| 7 | 表面张力测定方法的现状与进展显示文摘表面张力是影响各种化学反应和生物反应的关键因素之一,也是液体重要的物理性质。介绍了常见的几种测定表面张力的方法,包括毛细管上升法、最大气泡压力法、吊环法/吊片法、滴重法/滴体积法、振荡射流法、旋滴法和悬滴法,着重介绍了悬滴法用轴称滴形分析测定表面张力的进展。 | 奚新国 Pu Chen | 2008 | 盐城工学院学报(自然科学版)2008，21，3： | 23 |
| 8 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 9 | Genome Analysis of the Ancient Tracheophyte Selaginella tama#scina Reveals Evolutionary Features Relevant to the Acquisition of Desiccation Tolerance显示文摘 | Zhichao Xu Tianyi Xin Dorothea Bartels Ying Li Wei Gu Hui Yao Sai Liu Haoying Yu Xiangdong Pu Jianguo Zhou Jiang Xu Caicai xi Hetian Lei Jingyuan song Shilin Chen | 2018 | Molecular Plant2018，11，7： | 20 |
| 10 | The use of laser ranging to measure space debris显示文摘Space debris is a major problem for all the nations that are currently active in space.Adopting high-precision measuring techniques will help produce a reliable and accurate catalog for space debris and collision avoidance.Laser ranging is a kind of real-time measuring technology with high precision for space debris observation.The first space-debris laser-ranging experiment in China was performed at the Shanghai Observatory in July 2008 with a ranging precision of about 60-80 cm.The experimental results showed that the return signals from the targets with a range of 900 km were quite strong,with a power of 40W (2J at 20 Hz) using a 10ns pulse width laser at 532 nm wavelength.The performance of the preliminary laser ranging system and the observed results in 2008 and 2010 are also introduced. | Zhong-Ping Zhang 1,2,Fu-Min Yang 1,Hai-Feng Zhang 1,Zhi-Bo Wu 1,2,Ju-Ping Chen 1,2,Pu Li 1 and Wen-Dong Meng 1 1 Shanghai Astronomical Observatory,Chinese Academy of Sciences,Shanghai 200030,China 2 Key Laboratory of Space Object and Debris Observation,Chinese Academy of Science,Nanjing 210008,China | 2012 | Research in Astronomy and Astrophysics2012，12，2： | 20 |
| 11 | A Chromosome-Scale Genome Assembly of Paper Mulberry(Bmussonetia papyrifera)Provides New Insights into Its Forage and Papermaking Usage显示文摘Paper mulberry(Broussonetia papyrifera)is a well-known woody tree historically used for Cai Lun papermaking,one of the four great inventions of ancient China.More recently,Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents.In this study,we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches,including Illumina and PacBio sequencing platform as well as Hi-C,optical,and genetic maps.The assembled Paper mulberry genome consists of 386.83 Mb,which is close to the estimated size,and 99.25%(383.93 Mb)of the assembly was assigned to 13 pseudochromosomes.Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families,respectively,accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry.Moreover,the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine,forage,papermaking,and barkcloth making.We also identified the rootassociated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation.Collectively,these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition.Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making,and as forage.These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide. | Xianjun Peng Hui Liu Peilin Chen Feng Tang Yanmin Hu Fenfen Wang Zhi Pi Meiling Zhao Naizhi Chen Hui Chen Xiaokang Zhang Xueqing Yan Min Liu Xiaojun Fu Guofeng Zhao Pu Yao Lili Wang He Dai Xuming Li Wei Xiong Wencai Xu Hongkun Zheng Haiyan Yu Shihua Shen | 2019 | Molecular Plant2019，12，5： | 18 |
| 12 | Effectiveness of Different Waist Circumference Cut-off Values in Predicting Metabolic Syndrome Prevalence and Risk Factors in Adults in China显示文摘Objective To study the effectiveness of waist circumference cut-off values in predicting the prevalence of metabolic syndrome(MetS) and risk factors in adults in China. Methods A cross-sectional survey was condcuted in 14 provinces(autonomous region, municipality) in China. A total of 47 325 adults aged ≥20 years were selected by multistage stratified sampling, and questionnaire survey and physical and clinical examination were conducted among them. MetS was defined according to the International Diabetes Federation(IDF) criteria and modified IDF criteria.Results The age-standardized prevalence of MetS was 24.2%(22.1% in men and 25.8% in women) and 19.5%(22.1% in men and 18.0% in women) according to the IDF criteria and modified IDF criteria respectively. The age-standardized prevalence of pre-MetS was 8.1%(8.6% in men and 7.8% in women) according to the modified IDF criteria. The prevalence of MetS was higher in urban residents than rural residents and in northern China residents than in southern China residents. The prevalence of central obesity was about 30% in both men and women according to the ethnicity-specific cut-off values of waist circumference for central obesity(90 cm for men and 85 cm for women). Multivariate regression analysis revealed no significant difference in risk factors between the two MetS definitions. Conclusion Using both the modified IDF criteria and ethnicity-specific cut-off values of waist circumference can provide more useful information about the prevalence of MetS in China. | ZHOU Hai Cheng LAI Ya Xin SHAN Zhong Yan JIA Wei Ping YANG Wen Ying LU Ju Ming WENG Jian Ping JI Li Nong LIU Jie TIAN Hao Ming JI Qiu He ZHU Da Long CHEN Li GUO Xiao Hui ZHAO Zhi Gang Li Qiang ZHOU Zhi Guang GE Jia Pu SHAN Guang Liang | 2014 | Biomedical and Environmental Sciences2014，27，5： | 17 |
| 13 | Simple and cost-effective methods for precise analysis of trace element abundances in geological materials with ICP-MS显示文摘Inductively coupled plasma mass spectrometry(ICP-MS) is the most commonly used technique to determine the abundances of trace elements in a wide range of geological materials. However, incomplete sample digestion, isobaric interferences and instrumental drift remain obvious problems that must be overcome in order to obtain precise and accurate results. For this reason, we have done many experiments and developed a set of simple, cost-effective and practical methods widely applicable for precise and rapid determination of trace element abundances in geological materials using ICP-MS. Commonly used high-pressure digestion technique is indeed effective in decomposing refractory phases, but this inevitably produces fluoride complexes that create new problems. We demonstrate that the fluoride complexes formed during high-pressure digestion can be readily re-dissolved using high-pressure vessel at 190 °C for only 2 h for 50 mg sample. In the case of isobaric interferences, although oxide(e.g., MO+/M+) and hydroxide(e.g., MOH+/M+) productivity is variable between runs, the(MO+/M+)/(Ce O+/Ce+)and(MOH+/M+)/(Ce O+/Ce+) ratios remain constant, making isobaric interference correction for all other elements of interest straightforward, for which we provide an easy-to-use off-line procedure. We also show that mass-time-intensity drift curve is smooth as recognized previously, for which the correction can be readily done by analyzing a quality-control(QC) solution and using off-line Excel VBA procedure without internal standards. With these methods, we can produce data in reasonable agreement with recommended values of international rock reference standards with a relative error of <8% and precision generally better than 5%. Importantly, compared to the widely used analytical practice, we can effectively save >60% of time(e.g., <24 h vs. >60 h). | Shuo Chen Xiaohong Wang Yaoling Niu Pu Sun Meng Duan Yuanyuan Xiao Pengyuan Guo Hongmei Gong Guodong Wang Qiqi Xue | 2017 | Science Bulletin2017，62，4： | 17 |
| 14 | Efficacy and safety of Firebird sirolimus-eluting stent in treatment of complex coronary lesions in Chinese patients: one-year clinical and eight-month angiographic outcomes from the FIREMAN registry显示文摘 | LI Yan LI Cheng-xiang WANG Hai-chang XU Bo FANG Wei-yi GE Jun-bo WANG Wei-min CHEN Jack-P SHEN Wen-kuang JIANG Hong CONG Hong-liang PU Xiao-qun QIN Yong-wen JIN Hui-gen CAO Yu FIREMAN Investigators | 2011 | Chinese Medical Journal2011，，6： | 17 |
| 15 | Enteral nutrition and acute pancreatitis显示文摘INTRODUCTIONAcute pancreatitis (AP) is a common severe illness of the digestive tract with variable involvement of other regional tissues and / or remote organ sysems[1-3],Mild disease is associated with minimal organ dysfunction and rapid recovery ,while severe disease is associated with multiple organ system failure and local complications such as necrosis , abscess , fistulas and pseudocyst formation [4-6]. | Qiang Pu Chen Department of Heptobiliary Surgery, The Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China | 2001 | World Journal of Gastroenterology2001，7，2： | 15 |
| 16 | Dual Phosphorylation of Suppressor of Fused (Sufu) by PKA and GSK3 beta Regulates Its Stability and Localization in the Primary Cilium显示文摘 | Chen, Yon Yue, Shen Xie, Lu Pu, Xiao-hong Jin, Tion Cheng, Steven Y. | 2011 | 南京医科大学学报(自然科学版)2011，31，6： | 15 |
| 17 | Molecular mechanisms of apoptosis in hepatocellular carcinoma cells induced by ethanol extracts of Solanum lyratum Thumb through the mitochondrial pathway显示文摘AIM To explore the induction effects and mechanism of Solanum lyratum Thumb(ST) on human hepatocellularcarcinoma SMMC-7721 cells through the mitochondrial pathway.METHODS The experiments were conducted on three groups: an experimental group (with ST ethanol extracts' concentration being 2.5, 5 and 10 mg/L), a negative control group (with only nutrient solution, 0 mg/L ST ethanol extracts), and a positive control group (2.5 mg/L DDP). The inhibition rate of cell proliferation was checked by using the methyl thiazolyl tetrazolium method, and cell apoptosis was tested by TUNEL method. Furthermore, RT-PCR was used to examine m RNA expression of Fas, Fas L, caspase-8, caspase-3, p53 and Bcl-2 genes.RESULTS Compared with the negative control group, the inhibition and apoptosis rates of the experimental group with different concentrations of ST extracts on human hepatocellular carcinoma SMMC-7721 cells significantly increased(P<0.05). Besides, the m RNA expression of Fas L and Bcl-2 significantly decreased(P<0.05) while the m RNA expression of Fas, caspase-8, caspase-3 and p53 increased significantly. When compared with the positive control group, the experimental groups with 5 mg/L ST ethanol extracts showed effects similar to the positive control group.CONCLUSION ST ethanol extracts induced the apoptosis of hepatocellular carcinoma SMMC-7721 cells through up-regulated Fas, caspase-8, caspse-3 and p53, and down-regulated Fas L and Bcl-2 in the mitochondrial pathway. | Xiao-Qiang Mo Hong-Yu Wei Gan-Rong Huang Ling-Yuan Xu Yu-Li Chen Jiang Qi Wei Xian Yan-Chun Qin Lian-Deng Wei Li-Juan Zhao Yan-Qiang Huang Wei Xing Hong-Qin Pu Peng-Ya Wei Chao-Gan Li Qiu-Chun Liang | 2017 | World Journal of Gastroenterology2017，23，6： | 14 |
| 18 | De novo mutation in ATP6V1B2 impairs lysosome acidification and causes dominant deafness-onychodystrophy syndrome显示文摘 | Yongyi Yuan Jianguo Zhang Qing Chang Jin Zeng Feng Xin Jianjun Wang Qingyan Zhu Jing Wu Jingqiao Lu Weiwei Guo Xukun Yan Hui Jiang Binfei Zhou Qi Li Xue Gao Huijun Yuan Shiming Yang Dongyi Han Zixu Mao Ping Chen Xi Lin Pu Dai | 2014 | Cell Research2014，24，11： | 13 |
| 19 | The therapeutic effects of recombinant adenovirus RA538 on human gastric carcinoma cells in vitro and in vivo显示文摘AIM To evaluate the potential of RA-538 genetherapy for gastric carcinoma.METHODS Human gastric carcinoma cell lineSGC7901 treated with Ad-RA538 or Ad-LacZ wereanalysed by X-gal stain,MTT,DNA ladder,Tunel,flow cytometric analysis,PCR,andWestern Blot in vitro.The tumorigenicity andexperimental therapy in nude mice model wereassessed in vivo.RESULTS Ad-LacZ could efficiently transferthe LacZ gene into SGC7901 cells.X-gal-positivecells at MOI 25,50,100,and 200 were 90%,100%,100%,and 100% respectively.Ad-RA538could strongly inhibit cell growth and inducedapoptosis in SGC7901 cells.The proliferation ofthe Ad-RA538-infected SGC7901 cells wasreduced by 76.3%.The mechanism of killing ofgastric carcinoma cells by Ad-RA538 was foundto be apoptosis by DNA ladder,Tunel and flowcytometric analysis.The tumorigenicity in nudemice using Ad-RA538 showed that all three micefailed to form tumor from 7 to 30 days comparedwith Ad-LacZ and parent SGC7901 cells.Experimental therapy on the nude mice modelbearing subcutaneous tumor of SGC790| cells showed that intratumor instillation of Ad-RA538inhibited the growth of the tumors.Ad-RA538-treated tumors were inhibited by 60.66 %,compared with that of the tumor injected withAd-LacZ and mock.CONCLUSION The expression of Ad-RA538 can inhibit growth and induce apoptosis of gastric cancer cell in vitro and in vivo. Ad-RA538 can be used potentially in gene therapy for gastric carcinoma. | Jie Ping Chen Chen Lin Cai Pu Xu Xue Yan Zhang Ming Wu | 2000 | World Journal of Gastroenterology2000，6，6： | 12 |
| 20 | Expression of Bmi-1 and PAI-1 in esophageal squamous cell carcinoma显示文摘AIM:To determine the correlation between invasiveness,migration and prognosis in esophageal squamous cell carcinoma(ESCC)and expression of the B-cellspecific Moloney leukemia virus insert site 1(Bmi-1)and plasminogen activator inhibitor-1(PAI-1).METHODS:Eighty previously untreated patients who underwent surgical excision of ESCC were included.The expression of Bmi-1 and PAI-1 was examined immunohistochemically in formalin-fixed paraffinembedded primary tissue specimens.The relationships between the expression of Bmi-1 and PAI-1,the clinicopathologic features of ESCC,and the survival rate of ESCC patients were also discussed.The correlation between Bmi-1 and PAI-1 protein expression in ESCC was analyzed.The relationship between Bmi-1 and PAI-1expression and ESCC prognosis was evaluated using a Cox regression model and Kaplan-Meier survival curve analysis.RESULTS:The rates of positive Bmi-1 and PAI-1 expression in ESCC were higher than those in normal esophageal tissue(P<0.05).The expression of Bmi-1and PAI-1 was correlated with depth of invasion and lymph node metastasis(P<0.05),but not with patient age,tumor size or nationality(P>0.05).The expression of Bmi-1 was positively correlated with that of PAI-1(P<0.05).The 10-year overall survival rate for all patients was 20%(16∕80).Univariate KaplanMeier survival analysis showed that patients with high expression of esophageal PAI-1 and Bmi-1 had lower survival,however,the difference was not statistically significant.Cox multivariate analysis showed that PAI-1and Bmi-1 were not independent factors for survival rate,while the depth of tumor invasion and metastasis were independent factors affecting patient survival.CONCLUSION:The expression of Bmi-1 and PAI-1plays a role in ESCC progression,and may be used as a prognostic marker in ESCC. | Yan Zhang Ya-Lou Zhang Hong-Ming Chen Hong-Wei Pu Wen-Jing Ma Xiao-Mei Li Hong Ma Xiao Chen | 2014 | World Journal of Gastroenterology2014，20，18： | 12 |
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