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| 1 | Identification of a Stable Quantitative Trait Locus for Percentage Grains with White Chalkiness in Rice (Oryza sativa)显示文摘High chalkiness is a major problem in many rice-producing areas of the world, especially in hybrid rice (Oryza sativa L.) in China. We previously showed a major quantitative trait locus for the percentage of grains with white chalkiness (QTLqPGWC-8) in the interval G1149-R727 on chromosome 8 using a chromosome segment substitution line (CSSL). Here, we selected the line-CSSL50 harboring the QTLqPGWC-8 allele from the CSSLs derived from a cross between Asominori (as a recurrent parent) and IR24 (as a donor parent), which had higher percentage chalkiness, markedly different from that of Asominori. There were also significant differences in starch granules, appearance of amylose content (AAC) and milling qualities between Asominori and CSSL50, but not in grain size or thousand grain weight (TGW). The BC4F2 and BC4F3 populations from a cross between CSSL50 and Asominori were used for fine mapping of qPGWC-8. We narrowed down the location of this QTL to a 142 kb region between Indel markers 8G-7 and 8G-9. QTLqPGWC-8 accounted for 50.9% of the difference in PGWC between the parents. The markers tightly linked to qPGWC-8 should facilitate cloning of the gene underlying this QTL and will be of value for marker-assisted selection in breeding rice varieties with better grain quality. | Tao Guo Xiaolu Liu Xiangyuan Wan Jianfeng Weng Shijia Liu Xi Liu Mingjiang Chen Jingjing Li Ning Su Fuqing Wu Zhijun Cheng Xiuping Guo Cailin Lei Jiulin Wang Ling jiang Jianmin Wan | 2011 | Journal of Integrative Plant Biology2011，53，8： | 25 |
| 2 | A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice显示文摘The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI).However,the calcium channels responsible for this process have remained unknown.Here,we show that rice CDSI(CELL DEATH and SUSCEPTIBLE to BLAST 1)encoding OsCNGC9;a cyclic nucleotide-gated channel protein,positively regulates the resistance to rice blast disease.We show that OsCNGC9 mediates PAMP-induced Ca2+influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression.We further show that a PTI related receptor-lih cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity.Our results suggest a signaling cascade linking pattern recognition to calcium channel activation,which is required for initiation of PTI and disease resistance in rice. | Jiachang Wang Xi Liu An Zhang Yulong Ren Fuqing Wu Gang Wang Yang Xu Cailin Lei Shanshan Zhu Tian Pan Yongfei Wang Huan Zhang Fan Wang Yan-Qiu Tan Yupeng Wang Xin Jin Sheng Luo Chunlei Zhou Xiao Zhang Jinling Liu Shuai Wang Lingzhi Meng Yihua Wang Xi Chen Qibing Lin Xin Zhang Xiuping Guo Zhijun Cheng Jiulin Wang Yunlu Tian Shijia Liu Ling Jiang Chuanyin Wu Ertao Wang Jian-Min Zhou Yong-Fei Wang Haiyang Wang Jianmin Wan | 2019 | Cell Research2019，29，10： | 13 |
| 3 | γ-MnO2 nanorods/graphene composite as efficient cathode for advanced rechargeable aqueous zinc-ion battery显示文摘Aqueous Zn//MnO2 batteries are emerging as promising large-scale energy storage devices owing to their cost-effectiveness,high safety,high output voltage,and energy density.However,the MnO2 cathode suffers from intrinsically poor rate performance and rapid capacity deterioration.Here,we remove the roadblock by compositing MnO2 nanorods with highly conductive graphene,which remarkably enhances the electrochemical properties of the MnO2 cathode.Benefiting from the boosted electric conductivity and ion diffusion rate as well as the structural protection of graphene,the Zn//MnO2-graphene battery presents an admirable capacity of 301 mAh g^-1 at 0.5 A g^-1,corresponding to a high energy density of 411.6 Wh kg^-1.Even at a high current density of 10 A g^-1,a decent capacity of 95.8 mAh g^-1 is still obtained,manifesting its excellent rate property.Furthermore,an impressive power density of 15 kW kg^-1 is achieved by the Zn//MnO2-graphene battery. | Chao Wang Yinxiang Zeng Xiang Xiao Shijia Wu Guobin Zhong Kaiqi Xu Zengfu Wei Wei Su Xihong Lu | 2020 | Journal of Energy Chemistry2020，29，4： | 13 |
| 4 | Lattice-Based Double-Authentication-Preventing Ring Signature for Security and Privacy in Vehicular Ad-Hoc Networks显示文摘Amidst the rapid development of the Internet of Things (loT), Vehicular Ad-Hoc NETwork (VANET), a typical loT application, are bringing an ever-larger number of intelligent and convenient services to the daily lives of individuals. However, there remain challenges for VANETs in preserving privacy and security. In this paper, we propose the first lattice-based Double-Authentication-Preventing Ring Signature (DAPRS) and adopt it to propose a novel privacy-preserving authentication scheme for VANETs, offering the potential for security against quantum computers. The new construction is proven secure against chosen message attacks. Our scheme is more efficient than other ring signature in terms of the time cost of the message signing phase and verification phase, and also in terms of signature length. Analyses of security and efficiency demonstrate that our proposed scheme is provably secure and efficient in the application. | Jinhui Liu Yong Yu Jianwei Jia Shijia Wang Peiru Fan Houzhen Wang Huanguo Zhang | 2019 | Tsinghua Science and Technology2019，24，5： | 10 |
| 5 | SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer显示文摘SIRT7,a sirtuin family member implicated in aging and disease,is a regulator of metabolism and stress responses.It remains elusive how human somatic stem cell populations might be impacted by SIRT7.Here,we found that SIRT7 expression declines during human mesenchymal stem cell(hMSC)aging and that SIRT7 deficiency accelerates senescence.Mechanistically,SIRT7 forms a complex with nuclear lamina proteins and heterochromatin proteins,thus maintaining the repressive state of heterochromatin at nuclear periphery.Accordingly,deficiency of SIRT7 results in loss of heterochromatin,derepression of the LINE1 retrotransposon(LINE1),and activation of innate immune signaling via the cGAS-STING pathway.These agingassociated cellular defects were reversed by overexpression of heterochromatin proteins or treatment with a LINE1 targeted reverse-transcriptase inhibitor.Together,these findings highlight how SIRT7 safeguards chromatin architecture to control innate immune regulation and ensure geroprotection during stem cell aging. | Shijia Bi Zunpeng Liu Zeming Wu Zehua Wang Xiaoqian Liu Si Wang Jie Ren Yan Yao Weiqi Zhang Moshi Song Guang-Hui Liu Jing Qu | 2020 | Protein & Cell2020，11，7： | 10 |
| 6 | The Influence of Extractable Organic Matter on Pore Development in the Late Triassic Chang 7 Lacustrine Shales, Yanchang Formation, Ordos Basin, China显示文摘To investigate the influence of extractable organic matter(EOM) on pore evolution of lacustrine shales, Soxhlet extraction, using dichloromethane, was performed on a series of Chang 7 shale samples(Ordos Basin, China) with vitrinite reflectance of 0.64% to 1.34%. Low-pressure gas adsorption experiments were conducted on the samples before and after extraction. The pore structure parameters were calculated from the gas adsorption data. The results show complex changes to the pore volumes and surface areas after extraction. The pore development of both the initial and extracted samples is strongly controlled by total organic carbon(TOC) content. Micropores developed mainly in organic matter(OM), while mesopores and macropores predominantly developed in fractions other than OM. The influence of EOM on micropores is stronger than on mesopores and macropores. Organic solvents with a higher boiling point should be used to explore the effect of EOM on pore structure in the future. | HAN Hui LIU Pengwei DING Zhengang SHI Pitong JIA Jianchao ZHANG Wei LIU Yan CHEN Shijia LU Jungang Chen Kang PENG Xudong WANG Zhiyong XIAO Shuqi GAO Yuans | 2018 | Acta Geologica Sinica(English Edition)2018，92，4： | 8 |
| 7 | Interspecific Differences in Diet between Introduced Red-eared Sliders and Native Turtles in China显示文摘Although the alien Red-eared slider(Trachemys scripta elegans) is generally argued as an invasive species that competes with native turtles,the fi eld evidence on its diet is controversial.Field studies conducted at the Gutian Nature Reserve of Guangdong and along the Wanquan River in Hainan,China show that T.s.elegans is an opportunitic omnivore,consuming a wide variety of food items.Consumption varies throughout its range;more plant material is consumed in Guangdong,compared to a more carnivorous diet in Hainan;and juveniles are also found to have a more carnivorous diet than adults.This implies a high adaptability to new environment.Sympatric native Mauremys sinensis shows a more herbivorous diet and a narrower niche breadth(0.0260) in Hainan,plus a low niche overlap(0.3048) with T.s.elegans,providing a potential empty niche for the invasive T.s.elegans.Red-eared slider is also known to consume large quantities of native prey species to disrupt the ecological balance.It is imperative to understand the invasive nature of this species and the mechanisms by which it affects native ecology. | Jian WANG Haitao SHI Shijia HU Kai MA Chuang LI | 2013 | Asian Herpetological Research2013，4，3： | 7 |
| 8 | Uncommon EGFR mutations in a cohort of Chinese NSCLC patients and outcomes of first-line EGFR-TKIs and platinum-based chemotherapy显示文摘Objective: Data on the clinical activity of epidermal growth factor receptor(EGFR) tyrosine kinase inhibitors(TKIs) in patients with non-small-cell lung cancer(NSCLC) and uncommon EGFR mutations remain insufficient.This study aimed to investigate the effect of first-line EGFR-TKIs or platinum-based chemotherapy in NSCLC patients with uncommon EGFR mutations.Methods: We retrospectively enrolled 504 patients with EGFR-mutant NSCLC.The clinical characteristics and treatment outcomes were collected and compared between patients with common and uncommon EGFR-mutant NSCLC.Results: Seventy patients(13.9%) harboring uncommon EGFR mutations were included.Thirty of these patients received EGFR-TKIs and 40 received platinum-based chemotherapy as first-line therapy.The objective response rate(ORR) and median progression-free survival(m PFS) of patients treated with TKIs in the uncommon mutation group was significantly inferior to that in the common mutation group(ORR: 23.3% vs.51.8%,P=0.003; m PFS:7.1 vs.10.9 months,P<0.001).In the uncommon group,m PFS was similar between first-line EGFR-TKIs treatment and platinum-based chemotherapy(7.1 vs.6.1 months,P=0.893).In patients with EGFR G719 X or L861 Q mutations,the m PFS was longer in the first-line EGFR-TKIs treatment group than in the chemotherapy group,but the difference was not statistically significant(G719 X: 8.2 vs.5.8 months,P=0.061; L861 Q: 7.6 vs.4.1 months,P=0.872).Multivariate analyses identified adenocarcinoma(P=0.003) as the independent predictive factor for PFS in patients with uncommon EGFR mutations who were treated with first-line EGFR-TKIs.Conclusions: The current study demonstrated that the effect of first-line EGFR-TKIs was similar to that of platinum-based chemotherapy in patients with uncommon EGFR-mutant NSCLC.Adenocarcinoma was the independent predictive factor for PFS in uncommon EGFR-mutant NSCLC patients treated with first-line EGFRTKIs. | Jinpeng Shi Hui Yang Tao Jiang Xuefei Li Chao Zhao Limin Zhang Sha Zhao Xiaozhen Liu Yijun Jia Yan Wang Lei Xi Shijia Zhang Chunxia Su Shengxiang Ren Caicun Zhou | 2017 | Chinese Journal of Cancer Research2017，29，6： | 6 |
| 9 | Microstructure and composition engineering Yb single-filled CoSb_(3) for high thermoelectric and mechanical performances显示文摘A broad tunability of the thermoelectric and mechanical properties of CoSb_(3) has been demonstrated by adjusting the composition with the addition of an increasing number of elements.However,such a strategy may negatively impact processing repeatability and composition control.In this work,singleelement-filled skutterudite is engineered to have high thermoelectric and mechanical performances.Increased Yb filling fraction is found to increase phonon scattering,whereas cryogenic grinding contributes additional microstructural scattering.A peak zT of 1.55 and an average zT of about 1.09,which is comparable to the reported results of multiple-filled SKDs,are realized by the combination of simple composition and microstructure engineering.Furthermore,the mechanical properties of Yb single-filled CoSb_(3) skutterudite are improved by manipulation of the microstructure through cryogenic grinding.These findings highlight the realistic prospect of producing high-performance thermoelectric materials with reduced compositional complexity. | Zhenxing Zhou Matthias T.Agne Qihao Zhang Shun Wan Qingfeng Song Qing Xu Xiaofang Lu Shijia Gu Yuchi Fan Wan Jiang Gerald Jeffrey Snyder Lianjun Wang | 2019 | Journal of Materiomics2019，5，4： | 4 |
| 10 | Multidrug-resistant Proteus mirabilis isolates carrying blaOXA-1 and blaNDM-1 from wildlife in China:increasing public health risk显示文摘The emergence of multidrug resistance(MDR)in Proteus mirabilis clinical isolates is a growing public health concern and has serious implications for wildlife.What is the role of wildlife has been become one of the hot issues in disseminating antimicrobial resistance.Here,54 P.mirabilis isolates from 12 different species were identified.Among them,25 isolates were determined to be MDR by profile of antimicrobial susceptibility;10 MDR P.mirabilis isolates were subjected to comparative genomic analysis by whole genome sequencing.Comprehensive analysis showed that chromosome of P.mirabilis isolates mainly carries multidrug-resistance complex elements harboring resistance to carbapenem genes blaOXA-1,blaNDM-1,and blaTEM-1.Class I integron is the insertion hotspot of IS26;it can be inserted into type I integron at different sites,thus forming a variety of multiple drug resistance decision sites.At the same time,Tn21,Tn7,and SXT/R391 mobile elements cause widespread spread of these drug resistance genes.In conclusion,P.mirabilis isolates from wildlife showed higher resistance to commonly used clinic drugs comparing to those from human.Therefore,wild animals carrying MDR clinical isolates should be paid attention to by the public health. | Qian KANG Xue WANG Jianan ZHAO Zhihui LIU Fang JI Han CHANG Jianchun YANG Shijia HU Ting JIA Xiaojia WANG Jiagui TANG Guoying DONG Guocheng HU Jing WANG Yanyu ZHANG Jianhua QIN Chengmin WANG | 2021 | Integrative Zoology2021，16，6： | 4 |
| 11 | Spatial decoupling of light absorption and reaction sites in n-Si photocathodes for solar water splitting显示文摘Metal-insulator-semiconductor(MIS)photocathodes offer a simple alternative to p-n junction photocathodes in photoelectrochemical water splitting.However,the parasitic light absorption of catalysts and metal layers in the MIS junction,as well as the lack of low work function metals to form a large band offset with p-Si,severely limit their performance.This paper describes an MIS photocathode fabricated from n-Si,rather than the commonly used p-Si,to spatially decouple light absorption from reaction sites,which enables the majority carriers,instead of the commonly used minority carriers,to drive the surface reaction,making it possible to place the reaction sites far away from the light absorption region.Thus,the catalysts could be moved to the backside of the MIS junction to avoid light shielding.Moreover,the adoption of n-Si unlocks a variety of high work function materials for photovoltage generation.The obtained n-Si MIS photocathode exhibits an applied bias photon-to-current efficiency of 10.26%with a stability up to 300 h. | Shujie Wang Tuo Wang Bin Liu Huimin Li Shijia Feng Jinlong Gong | 2021 | National Science Review2021，8，8： | 2 |
| 12 | Realization of warm white light emitting in single phase Gd(P_(x)V_(1−x))O_(4):y at% Sm^(3+),1 at% Bi^(3+) phosphor显示文摘A series of single phase,warm white light emitting phosphors,Gd(P_(x)V_(1−x))O_(4):y at%Sm^(3+),with 1 at%Bi^(3+) doping concentration were synthesized by high temperature solid state method in this work.The experimental results indicate broadband cyan emission of Bi^(3+)and characteristic orange-red emission of Sm^(3+)can be effectively tuned by changing the ratios of PO^(3−)_(4)/VO^(3−)_(4) in Gd(P_(x)V_(1−x))O_(4):1 at%Sm^(3+),1 at%Bi^(3+),and the energy transfer process among VO^(3−)_(4),Sm^(3+),Bi^(3+) also can be adjusted.Based on this,warm white light emitting can be realized by further optimizing the doping concentration of Sm^(3+) in the phosphors.At 423 K,the PL intensity of Gd(P_(0.7)V_(0.3))O_(4):2 at%Sm^(3+),1 at%Bi^(3+) remains~84.3%of the initial value at 293 K,while the measured quantum efficiency is 67.8%.EL spectrum analysis results of the fabricated white light emitting diode(wLED)based on a 310 nm UV-chip and Gd(P_(0.7)V_(0.3))O4:2 at%Sm^(3+),1 at%Bi^(3+)phosphors imply low correlated color temperature(3132 K)and appropriate color-rending index(R_(a)=82.7).These results demonstrate that Gd(P_(0.7)V_(0.3))O4:2 at%Sm^(3+),1 at%Bi^(3+)is a good candidate for manufacturing UV-activated warm white light emitting diodes. | Chao Dou Yanzhen Yin Fei Zheng Zhen Wang Shijia Sun Chen Hu Yang Che Bing Teng Degao Zhong | 2022 | Journal of Rare Earths2022，40，4： | 2 |
| 13 | Enhanced thermoelectric properties of hydrothermally synthesized n-type Se&Lu-codoped Bi2Te3显示文摘N-type Se&Lu-codoped Bi2Te3 nanopowders were prepared by hydrothermal method and sintered by spark plasma sintering technology to form dense samples.By further doping Se element into Lu-doped Bi2Te3 samples,the thickness of the nanosheets has the tendency to become thinner.The electrical conductivity of Lu0.1Bi1.9Te3−xSex material is reduced with the increasing Se content due to the reduced carrier concentration,while the Seeback coefficient values are enhanced.The lattice thermal conductivity of the Lu0.1Bi1.9Te3−xSex is greatly reduced due to the introduced point defects and atomic mass fluctuation.Finally,the Lu0.1Bi1.9Te2.7Se0.3 sample obtained a maximum ZT value of 0.85 at 420 K.This study provides a low-cost and simple low-temperature method to mass production of Se&Lu-codoped Bi2Te3 with high thermoelectric performance for practical applications. | Xiaolei Shi Xin Ai Qihao Zhang Xiaofang Lu Shijia Gu Li Su Lianjun Wang Wan Jiang | 2020 | Journal of Advanced Ceramics2020，9，4： | 2 |
| 14 | Selection, identification, and application of Aflatoxin B1 aptamer显示文摘 | Xiaoyuan Ma Wenfeng Wang Xiujuan Chen Yu Xia Shijia Wu Nuo Duan Zhouping Wang | 2014 | European Food Research and Technology2014，，6： | 1 |
| 15 | Enhanced TE properties of Cu@Ag/Bi2Te3 nanocomposites by decoupling electrical and thermal properties显示文摘Cu@Ag/Bi2Te3 nanocomposites were prepared for the first time by ultrasonic dispersion-rapid freezedrying method combined with spark plasma sintering(SPS).By changing the content of Cu@Ag nanoparticle,we could modulate the temperature dependent thermoelectric properties.The highest ZT value can be obtained at 450 K for 1 vol%Cu@Ag/Bi2Te3,which is benefited from the decoupling of electrical and thermal properties.With the increase of electrical conductivity,the absolute value of Seebeck coefficient lifts while the thermal conductivity declines.Meanwhile,the average ZT value between 300 K and 475 K was 0.61 for 1 vol%Cu@Ag/Bi2Te3,which is much higher than that of pristine Bi2 Te3.Therefore,the decoupling effect of Cu@Ag nanoparticles incorporation could be a promising method to broaden the application of Bi2Te3 based thermoelectric materials. | Xiaofang Lu Qi Zheng Shijia Gu Rui Guo Li Su Jiancheng Wang Zhenxing Zhou Yuchi Fan Wan Jiang Lianjun Wang | 2020 | Chinese Chemical Letters2020，31，3： | 1 |
| 16 | Ribonuclease H-like gene SMALL GRAIN2 regulates grain size in rice through brassinosteroid signaling pathway显示文摘Grain size is a key agronomic trait that determines the yield in plants.Regulation of grain size by brassinosteroids(BRs)in rice has been widely reported.However,the relationship between the BR signaling pathway and grain size still requires further study.Here,we isolated a rice mutant,named small grain2(sg2),which displayed smaller grain and a semi-dwarf phenotype.The decreased grain size was caused by repressed cell expansion in spikelet hulls of the sg2 mutant.Using map-based cloning combined with a Mut Map approach,we cloned SG2,which encodes a plant-specific protein with a ribonuclease H-like domain.SG2 is a positive regulator downstream of GLYCOGEN SYNTHASE KINASE2(GSK2)in response to BR signaling,and its mutation causes insensitivity to exogenous BR treatment.Genetical and biochemical analysis showed that GSK2 interacts with and phosphorylates SG2.We further found that BRs enhance the accumulation of SG2 in the nucleus,and subcellular distribution of SG2 is regulated by GSK2 kinase activity.In addition,Oryza sativa OVATE family protein 19(OsOFP19),a negative regulator of grain shape,interacts with SG2 and plays an antagonistic role with SG2 in controlling gene expression and grain size.Our results indicated that SG2 is a new component of GSK2-related BR signaling response and regulates grain size by interacting with Os OFP19. | Yunshuai Huang Hui Dong Changling Mou Ping Wang Qixian Hao Min Zhang Hongmin Wu Fulin Zhang Tengfei Ma Rong Miao Kai Fu Yaping Chen Ziyan Zhu Cheng Chen Qikai Tong Zhuoran Wang Shirong Zhou Xi Liu Shijia Liu Yunlu Tian Ling Jiang Jianmin Wan | 2022 | Journal of Integrative Plant Biology2022，64，10： | 1 |
| 17 | Conservation of weathered historic sandstone with biomimetic apatite显示文摘The conservation of weathered historic sandstone with apatite was studied.Based on the growth mechanism of bone,calcium and phosphorus were introduced into weathered sandstone and then mineralized at room temperature.The conservation efficiency was investigated by scanning electron microscopy(SEM),energy-dispersive X-ray spectroscopy(EDX),transmission electron microscopy(TEM),selected area electron diffraction(SAED),X-ray diffraction(XRD),and compressive strength,capillary water uptake,water vapor permeability and weather resistance tests.The SEM,EDX,TEM,SAED and XRD results showed that the produced hydroxyapatite could reunite the weathered stone blocks and provide sufficient reinforcement to hold them together.The results of the various tests indicated that the compressive strength and weather resistance of the treated samples was improved significantly.In addition,because of the porous nature of apatite,the water vapor permeability of the treated stone was not affected and its breathability was maintained. | YANG FuWei LIU Yan ZHU YuanCheng LONG ShiJia ZUO GuoFang WANG ChangQing GUO Feng ZHANG BingJian JIANG SiWei | 2012 | Chinese Science Bulletin2012，57，17： | 1 |
| 18 | A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels显示文摘 | Nuo Duan Shijia Wu Ye Yu Xiaoyuan Ma Yu Xia Xiujuan Chen Yukun Huang Zhouping Wang | 2013 | Analytica Chimica Acta2013，，： | 1 |
| 19 | CsWRKY13,a novel WRKY transcription factor of Camellia sinensis,involved in lignin biosynthesis and accumulation显示文摘Lignin is an aromatic polymer that provides the necessary mechanical strength for the transport of water and nutrients in higher plants.Lignin biosynthesis and accumulation affect growth and development of tea plants.The degree of lignification related to the tenderness of fresh tea leaves determines the quality of tea.WRKY transcription factors play central roles in plant development and physiological processes.However,the roles of WRKY transcription factors in lignin biosynthesis of tea plants remain unclear.In this study,a WRKY gene,CsWRKY13,was cloned from tea plant'Longjing 43'.The open reading frame(ORF)of CsWRKY13 gene was 708 bp,encoding 235 amino acids.Sequence analysis showed that CsWRKY13 contained a conserved WRKYGQK amino acid sequence and a zinc-finger-like motif CX4CX23HXH.Subcellular localization showed that CsWRKY13 was localized in the nucleus.The yeast trans-activation assay showed that CsWRKY13 had no transcriptional activity.Expression analysis showed that the CsWRKY13 gene was highly expressed in the stem.Overexpression of CsWRKY13 in Arabidopsis thaliana reduced lignin content and the expression levels of genes related to lignin biosynthesis in transgenic plants.Most flavonoids pathway related genes were significantly up-regulated.This study shows that CsWRKY13 might function as a negative regulator in regulation of lignin synthesis. | Ruimin Teng Yongxin Wang Shijia Lin Yi Chen Yazhuo Yang Ni Yang Jingwen Li Jing Zhuang | 2021 | Beverage Plant Research2021，1，1： | 1 |
| 20 | Design and fabrication of r-hirudin loaded dissolving microneedle patch for minimally invasive and long-term treatment of thromboembolic disease显示文摘Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potentially prevent thrombosis via subcutaneous(SC)and intravenous(IV)administration,but there is a risk of haemorrhage via SC and IV.Thus,microneedle(MN)provides painless and sanitary alternatives to syringes and oral administration.However,the current technological process for the micro mould is complicated and expensive.The micro mould obtained via three-dimensional(3D)printing is expected to save time and cost,as well as provide a diverse range of MNs.Therefore,we explored a method for MNs array model production based on 3D printing and translate it to micro mould that can be used for fabrication of dissolving MNs patch.The results show that r-hirudin-loaded and hyaluronic acid(HA)-based MNs can achieve transdermal drug delivery and exhibit significant potential in the prevention of thromboembolic disease without bleeding in animal models.These results indicate that based on 3D printing technology,MNs combined with r-hirudin are expected to achieve diverse customizableMNs and thus realize personalized transdermal anticoagulant delivery for minimally invasive and long-term treatment of thrombotic disease. | Mengfang Wu Tian Xia Yaran Li Tianfa Wang Shijia Yang Jinchao Yu Qiaoyan Liang Teng Shen Min Yu Bing Zhao | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，2： | 1 |
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