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| 1 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | Charge Transport in Oil Impregnated Paper Insulation Under Temperature Gradient Using Transient Upstream FEM显示文摘Charge transport in oil impregnated paper impacts the insulation performance of a transformer.This paper proposes a simulation method for the charge transport in oil impregnated paper insulation.The transient upstream finite element method(FEM)is applied to the transport equations of bipolar charges for establishing a numerical simulation model of charge transport in oil impregnated paper insulation.The method is validated by experimental results.The charge transport and electric field distribution in single-layer oil impregnated paper insulation under different temperature gradients is simulated.The trends of the simulation results are seen to agree with the corresponding experimental results.This paper conducts exploratory research into the simulation of charge transportation phenomenon in oil impregnated paper,and is of importance to the design of oil impregnated paper insulation. | Shuo Jin Jiangjun Ruan Zhiye Du Zhifei Yang Taotao Zhou Guodong Huang Lin Zhu | 2015 | CSEE Journal of Power and Energy Systems2015，1，3： | 10 |
| 3 | Design of Eco-friendly Ultra-high Performance Concrete with Supplementary Cementitious Materials and Coarse Aggregate显示文摘Aiming to investigate the mix design of eco-friendly UHPC with supplementary cementitious materials and coarser aggregates, we comprehensively studied the workability, microstructure, porosity, compressive strength, flexural strength, and Young’s modulus of UHPC. Relationship between compressive strength and Young’s modulus was obtained eventually. It is found that the compressive strength, flexural strength, and Young’s modulus of UHPC increase by 19.01%, 10.81%, and 5.99%, respectively, when 40 wt% cement is replaced with supplementary cementitious materials. The relationship between compressive strength and Young’s modulus of UHPC is an exponential form. | 蒋金洋 ZHOU Wenjing CHU Hongyan WANG Fengjuan WANG Liguo FENG Taotao GUO Dong | 2019 | Journal of Wuhan University of Technology(Materials Science)2019，34，6： | 3 |
| 4 | A specific aggregation-induced emission-conjugated polymer enables visual monitoring of osteogenic differentiation显示文摘Osteogenic differentiation is the basis of bone growth and repair related to many diseases,in which evaluating the degree and ability of osteogenic transformation is quite important and highly desirable.However,fixing or stopping the growth of cells is required for conventional methods to monitor osteogenic differentiation,which cannot realize the full investigation of the dynamic process.Herein,a new anion conjugated polymer featuring aggregation-induced emission(AIE)characteristics is developed with excellent solubility for in-situ monitoring the process of osteogenic differentiation.This novel polymer can bind with osteogenic differentiated cells,and the intracellular fluorescence increases gradually with the enhancement of osteogenic differentiation.Moreover,it possesses good biosafety with negligible effect on cell activity and osteogenic differentiation,which cannot be realized by the typical method of Alizarin Red S staining.Further study shows that the polymer crosses the cell membrane through endocytosis and enriches in lysosomes,whereas no obvious fluorescence is detected with other cells,including non-differentiated osteoblast cells,under the same conditions,demonstrating the high selectivity.This is the first fluorescent probe with excellent specificity to realize real-time observation of the process of osteogenic differentiation.Therefore,PTB-EDTA shows great promise in the study of osteogenic differentiation and related applications. | Zhenyu Zheng Taotao Zhou Rong Hu Minjun Huang Xiang Ao Jun Chu Tao Jiang Anjun Qin Zhongmin Zhang | 2020 | Bioactive Materials2020，5，4： | 3 |
| 5 | Lab-in-cell based on spontaneous amino-yne click polymerization显示文摘Unnatural reaction in the living cells is a powerful tool for biological research. However, the polymerization inside cells is rarely reported. In this work, a lab-in-cell is illustrated based on our developed spontaneous amino-yne click polymerization. Carbonyl group activated terminal diyne can spontaneously polymerize with tetraphenylethene(TPE)-containing primary diamine inside cells, and polymer with weight-average molecular weight of 7,300 was yielded. By utilizing this in vivo amino-yne click polymerization and taking advantage of the aggregation-induced emission feature of TPE, a 'turn-on' cell imaging was realized,and in-situ killing of cells was also acquired by destroying the structures of actin and tubulin, which cannot be realized by preprepared polymer. This strategy provides a useful platform and holds great promise in biochemistry and therapy applications. | Rong Hu Xu Chen Taotao Zhou Han Si Benzhao He Ryan T. K. Kwok Anjun Qin Ben Zhong Tang | 2019 | Science China Chemistry2019，62，9： | 1 |
| 6 | Author Correction:Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing显示文摘Correction to:Cell Research https://doi.org/10.1038/s41422-022-00635-9,published online 10 March 2022 We found that the information in the original Supplementary Figs.S2–S4 was not fully displayed due to an error in the image upload.The correct images are shown below for better display.These corrections do not affect the description of the results or the conclusion of this work.No change of the original figure legends is needed.We apologize for the mistake.The original article has been corrected. | Ziqi Zhao Dan Zhang Fuqiang Yang Mingrui Xu Shaoli Zhao Taotao Pan Chuanyu Liu Yongjie Liu Qingfeng Wu Qiang Tu Ping Zhou Rong Li Jia Kang Lan Zhu Fei Gao Yaqing Wang Zhiheng Xu | 2023 | Cell Research2023，33，7： | 1 |
| 7 | Experimental study of the settling of twin spherical particles released side by side: The impact of particle size, fluid viscosity, initial spacing, and particle density显示文摘The settling of solid particles in a fluid is an important process that needs to be considered in many fields of research.For example,the interactions among particles and between particles and the surrounding fluid are important topics in studying suspended sediment transport and water clarification.In this paper,the settling processes and interactions of twin spherical particles released side by side were experimentally studied.The Reynolds number varied in the range of 1-300,which is within the transition zone.Particle Tracking Velocimetry(PTV)and Particle Image Velocimetry(PIV)were utilized to capture the settlement trajectory,and provide insight into the flow fields around the particles.The influences of particle size,fluid viscosity,initial spacing,and particle density on the settling process were systematically investigated.The experimental results reveal that the initial spacing between the twin particles(l_(0)*)and the Reynolds number(Re)are the two most important factors affecting particle settling.The interaction between particles comprises only repulsion when the initial spacing is small,while the density of particles has little effect on the final settling state when the initial spacing is not very small.The flow fields around different particles are similar for the same Re,leading to similar final settlement behaviors,except for the case of l_(0)*=0,when the influence of particle rotation cannot be ignored.Except for the case of l_(0)*=0,although the particle density has little effect on the final settling behavior,it affects the repulsive process during settling.The final repulsive distance between the twin particles is highly dependent on Re and l_(0)*.Two critical Re values exist(~10 and~100),where the repulsive distance is negatively correlated with Re when Re<10,but it is positively correlated with Re when Re>100.However,the repulsive distance is always negatively correlated with l_(0)*.These findings can improve understanding of more complex phenomena such as the particle group settling process and sediment transport. | Yang Xiao Jieqing Liu Pei Zhang Jian Zhou Dongfang Liang Zhihao Wang Taotao Zhang Saiyu Yuan Hongwu Tang | 2023 | International Journal of Sediment Research2023，38，1： | 1 |
| 8 | Influence of Ce/Cu ratio on the performance of ordered mesoporous CeCu composite oxide catalysts显示文摘 | Guilin Zhou Hai Lan Taotao Gao Hongmei Xie | 2014 | Chemical Engineering Journal2014，，： | 1 |
| 9 | Enzymatic formation of curcumin in vitro and in vivo显示文摘 | Jiaqing wang Taotao Xiong Jie Zhou Hongjian He Dongdong Wu Xuewen Du Xingyi Li Bing Xu | 2018 | Nano Research2018，11，6： | 1 |
| 10 | Highly selective oxidation of amines to imines by Mn2O3 catalyst under eco-friendly conditions显示文摘Enhancing the selectivity of imines for the oxidative self-coupling of primary amines was found to be challenging in the heterogeneous catalysis.Three different manganese oxides(M-3,M-4,M-5) were synthesized by controlling the calcination temperature using a simple template-free oxalate route.The prepared manganese oxides were systematically characterized using XRD,N2 sorption,SEM,TEM,XPS,H2-TPR techniques.M-4 gave 96.2% selectivity of imine at 100% conversion of benzylamine,which was far more superior than other existing protocols.Mn^3+/Mn^4+ ratio was found to affect the selectivity of the imines.The probable reaction pathway for amines oxidation catalyzed by manganese oxides was proposed for the first time. | Fushan Chen Tao Yang Songlin Zhao Taotao Jiang Lu Yu Houfeng Xiong Chuankun Guo Yufang Rao Yan Liu Liu Liu Jian Zhou Pengxiang Tu Jun Ni Qunfeng Zhang Xiaonian Li | 2019 | Chinese Chemical Letters2019，30，12： | 1 |
| 11 | Plasma membrane calcium ATPase 4b inhibits nitric oxide generation through calcium-induced dynamic interaction with neuronal nitric oxide synthase显示文摘The activation and deactivation of Ca^(2+)- and calmodulindependent neuronal nitric oxide synthase (nNOS) in the central nervous system must be tightly controlled to prevent excessive nitric oxide (NO) generation. Considering plasma membrane calcium ATPase (PMCA) is a key deactivator of nNOS, the present investigation aims to determine the key events involved in nNOS deactivation of by PMCA in living cells to maintain its cellular context. Using time-resolved Förster resonance energy transfer (FRET), we determined the occurrence of Ca^(2+)-induced protein-protein interactions between plasma membrane calcium ATPase 4b (PMCA4b) and nNOS in living cells. PMCA activation significantly decreased the intracellular Ca 2+ concentrations ([Ca^(2+)]_(i)), which deactivates nNOS and slowdowns NO synthesis. Under the basal [Ca^(2+)]_(i) caused by PMCA activation, no protein-protein interactions were observed between PMCA4b and nNOS. Furthermore, both the PDZ domain of nNOS and the PDZ-binding motif of PMCA4b were essential for the protein-protein interaction. The involvement of lipid raft microdomains on the activity of PMCA4b and nNOS was also investigated. Unlike other PMCA isoforms, PMCA4 was relatively more concentrated in the raft fractions. Disruption of lipid rafts altered the intracellular localization of PMCA4b and affected the interaction between PMCA4b and nNOS, which suggest that the unique lipid raft distribution of PMCA4 may be responsible for its regulation of nNOS activity. In summary, lipid rafts may act as platforms for the PMCA4b regulation of nNOS activity and the transient tethering of nNOS to PMCA4b is responsible for rapid nNOS deactivation. | Wenjuan Duan Juefei Zhou Wei Li Teng Zhou Qianqian Chen Fuyu Yang Taotao Wei | 2013 | Protein & Cell2013，4，4： | 1 |
| 12 | Influence of Ce/Cu Ratio on the Performance of Ordered Mesoporous Ce Cu Composite Oxide Catalysts显示文摘 | Zhou Guilin Lan Han Gao Taotao | 2014 | Chem Eng J2014，246，： | 1 |
| 13 | Scientific objectives of the Hot Universe Baryon Surveyor(HUBS) mission显示文摘The Hot Universe Baryon Surveyor(HUBS) is a proposed space-based X-ray telescope for detecting X-ray emissions from the hot gas content in our universe. With its unprecedented spatially-resolved high-resolution spectroscopy and large field of view,the HUBS mission will be uniquely qualified to measure the physical and chemical properties of the hot gas in the interstellar medium, the circumgalactic medium, the intergalactic medium, and the intracluster medium. These measurements will be valuable for two key scientific goals of HUBS, namely to unravel the AGN and stellar feedback physics that governs the formation and evolution of galaxies, and to probe the baryon budget and multi-phase states from galactic to cosmological scales. In addition to these two goals, the HUBS mission will also help us solve some problems in the fields of galaxy clusters, AGNs, difuse X-ray backgrounds, supernova remnants, and compact objects. This paper discusses the perspective of advancing these fields using the HUBS telescope. | Joel Bregman Renyue Cen Yang Chen Wei Cui Taotao Fang Fulai Guo Edmund Hodges-Kluck Rui Huang Luis C.Ho Li Ji Suoqing Ji Xi Kang Xiaoyu Lai Hui Li Jiangtao Li Miao Li Xiangdong Li Yuan Li Zhaosheng Li Guiyun Liang Helei Liu Wenhao Liu Fangjun Lu Junjie Mao Gabriele Ponti Zhijie Qu Chenxi Shan Lijing Shao Fangzheng Shi Xinwen Shu Lei Sun Mouyuan Sun Hao Tong Junfeng Wang Junxian Wang Q.Daniel Wang Song Wang Tinggui Wang Weiyang Wang Zhongxiang Wang Dandan Xu Haiguang Xu Heng Xu Renxin Xu Xiaojie Xu Yongquan Xue Hang Yang Feng Yuan Shuinai Zhang Yuning Zhang Zhongli Zhang Yuanyuan Zhao Enping Zhou Ping Zhou | 2023 | Science China(Physics,Mechanics & Astronomy)2023，66，9： | 1 |
| 14 | Transcriptomic analysis of human breast cancer cells reveals differentially expressed genes and related cellular functions and pathways in response to gold nanorods显示文摘 | Teng Zhou Yipeng Du Taotao Wei | 2015 | Biophysics Reports2015，1，2： | 1 |
| 15 | Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing显示文摘The differences in size and function between primate and rodent brains,and the association of disturbed excitatory/inhibitory balance with many neurodevelopmental disorders highlight the importance to study primate ganglionic eminences(GEs)development.Here we used single-cell RNA and ATAC sequencing to characterize the emergence of cell diversity in monkey and human GEs where most striatal and cortical interneurons are generated.We identified regional and temporal diversity among progenitor cells which give rise to a variety of interneurons.These cells are specified within the primate GEs by well conserved gene regulatory networks,similar to those identified in mice.However,we detected,in human,several novel regulatory pathways or factors involved in the specification and migration of interneurons.Importantly,comparison of progenitors between our human and published mouse GE datasets led to the discovery and confirmation of outer radial glial cells in GEs in human cortex.Our findings reveal both evolutionarily conservative and nonconservative regulatory networks in primate GEs,which may contribute to their larger brain sizes and more complex neural networks compared with mouse. | Ziqi Zhao Dan Zhang Fuqiang Yang Mingrui Xu Shaoli Zhao Taotao Pan Chuanyu Liu Yongjie Liu Qingfeng Wu Qiang Tu Ping Zhou Rong Li Jia Kang Lan Zhu Fei Gao Yaqing Wang Zhiheng Xu | 2022 | Cell Research2022，32，5： | 1 |
| 16 | KLF4 facilitates chromatin accessibility remodeling in porcine early embryos显示文摘Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early embryos.Current studies have illustrated several pioneer factors as being important for agricultural animals,but what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos is not clear.By employing low-input DNase-seq(liDNase-seq),we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation development.Our data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early embryos.Knocking down of KLF4 led to the reduction of chromatin accessibility in early embryos,whereas KLF4 overexpression promoted the chromatin openness in porcine blastocysts.Furthermore,KLF4 deficiency resulted in mitochondrial dysfunction and developmental failure of porcine embryos.In addition,we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation,whereas knockdown of KLF4 disrupted this process.Taken together,our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo development. | Wei Zhu Guowei Bu Ruifeng Hu Jixiang Zhang Lianyong Qiao Kai Zhou Tingting Wang Qiao Li Jingjing Zhang Linhui Wu Yali Xie Taotao Hu Shichun Yang Jiaqi Guan Xiaoyu Chu Juanjuan Shi Xia Zhang Falong Lu Xin Liu Yi-Liang Miao | 2024 | Science China(Life Sciences)2024，67，1： | 0 |
| 17 | Identification of lipid droplets in gut bacteria显示文摘Dear Editor,The field of gut microbiota is progressing rapidly and thus,appellations like the last human organ,a separate organ,a forgotten organ,and a new organ have been applied to gut microbiota to emphasize its vital functions in our body(Chang and Kao,2019).Gut microbiota has been shown to play a central role in the regulation of human lipid metabolism and be associated with lipid metabolism disorders when aberrant,through composition and microbial metabolites. | Kai Zhang Chang Zhou Zemin Li Xuehan Li Ziyun Zhou Linjia Cheng Ahmed Hammad Mirza Yumeng Shi Bingbing Chen Mengwei Zhang Liujuan Cui Congyan Zhang Taotao Wei Xuelin Zhang Shuyan Zhang Pingsheng Liu | 2023 | Protein & Cell2023，14，2： | 0 |
| 18 | Textual Content Prediction via Fuzzy Attention Neural Network Model without Predefined Knowledge显示文摘Text analysis is a popular technique for finding the most significant information from texts including semantic,emotional,and other hidden features,which became a research hotspot in the last few years.Specially,there are some text analysis tasks with judgment reports,such as analyzing the criminal process and predicting prison terms.Traditional researches on text analysis are generally based on special feature selection and ontology model generation or require legal experts to provide external knowledge.All these methods require a lot of time and labor costs.Therefore,in this paper,we use textual data such as judgment reports creatively to perform prison term prediction without external legal knowledge.We propose a framework that combines value-based rules and a fuzzy text to predict the target prison term.The procedure in our framework includes information extraction,term fuzzification,and document vector regression.We carry out experiments with real-world judgment reports and compare our model’s performance with those of ten traditional classification and regression models and two deep learning models.The results show that our model achieves competitive results compared with other models as evaluated by the RMSE and R-squared metrics.Finally,we implement a prototype system with a user-friendly GUI that can be used to predict prison terms according to the legal text inputted by the user. | Canghong Jin Guangjie Zhang Minghui Wu Shengli Zhou Taotao Fu | 2020 | China Communications2020，17，6： | 0 |
| 19 | ZmMS1/ZmLBD30-orchestrated transcriptional regulatory networks precisely control pollen exine development显示文摘Because of its significance for plant male fertility and,hence,direct impact on crop yield,pollen exine development has inspired decades of scientific inquiry.However,the molecularmechanismunderlying exine formation and thickness remains elusive.In this study,we identified that a previously unrecognized repressor,ZmMS1/ZmLBD30,controls proper pollen exine development in maize.Using an ms1 mutant with aberrantly thickened exine,we cloned a male-sterility gene,ZmMs1,which encodes a tapetum-specific lateral organ boundary domain transcription factor,ZmLBD30.Weshowed thatZmMs1/ZmLBD30 is initially turned on by a transcriptional activation cascade of ZmbHLH51-ZmMYB84-ZmMS7,and then it serves as a repressor to shut down this cascade via feedback repression to ensure timely tapetal degeneration and proper level of exine.This activation-feedback repression loop regulating male fertility is conserved in maize and sorghum,and similar regulatory mechanism may also exist in other flowering plants such as rice and Arabidopsis.Collectively,these findings reveal a novel regulatory mechanism of pollen exine development by which a long-sought master repressor of upstream activators prevents excessive exine formation. | Quancan Hou Xueli An Biao Ma Suowei Wu Xun Wei Tingwei Yan Yan Zhou Taotao Zhu Ke Xie Danfeng Zhang Ziwen Li Lina Zhao Canfang Niu Yan Long Chang Liu WeiZhao FeiNi Jinping Li Daolin Fu Zhong-NanYang Xiangyuan Wan | 2023 | Molecular Plant2023，16，8： | 0 |
| 20 | miRNA-141-5p Affects the Levels of Neutrophil Elastase in Preeclampsia by Regulating MAPK1显示文摘Objective:The objective of this study was to investigate the expression levels of microRNA-141-5p(miRNA-141-5p),MAPK1 and neutrophil elastase in patients with and without preeclampsia(PE),and the relationship between miRNA-141-5p and MAPK1 with respect to the secretion of elastase by neutrophils in patients with PE.Methods:Thirty patients with PE and 30 healthy pregnant(HP)women were recruited from The Second Hospital of Shanxi Medical University,Taiyuan,China,between February 2017 and July 2018.Neutrophils were isolated from 8 mL peripheral blood samples and cultured.We recorded neutrophil count and morphology during culture.Apoptosis was detected by flow cytometry in different groups at 0,24,and 48 h.The expression levels of elastase were detected in neutrophils by enzyme-linked immunosorbent assay,whereas the expression levels of miRNA-141-5p in peripheral blood neutrophils were detected by real-time polymerase chain reaction.We used TargetScanHuman Release 7.2 to analyze the target genes of miRNA-141-5p.The expression of MAPK1 in peripheral blood neutrophils was detected by western blotting.Data were analyzed by SPSS version 21.0 software,and comparisons between groups were carried out with the Studentt test.Results:There was no significant difference between the PE and HP groups(P>0.050)with regard to age or body mass index.The weight of newborns in the PE group(2846.00±600.00 g)was significantly lower than that in the HP group(3055.00±230.68 g).The number of neutrophilic granulocytes(NGs)in blood samples from the PE group was significantly higher than that in the HP group(P=0.003).There was no significant difference between the groups with regard to morphology.Apoptosis in the PE group was delayed when compared with the HP group at different time points.TheP value of apoptosis in the PE and HP groups were respectively 0.790,<0.001 and 0.030 at 0 h,24 h and 48 h.The expression levels of miRNA-141-5p in the PE group were significantly lower than those in the HP group(P<0.050).The expression levels of MAPK1 in neutrophils from the PE group were significantly higher than those in the HP group(P<0.050)by western blot.The expression levels of elastase in neutrophils from the PE group were significantly higher than those in the HP group(P<0.050).Furthermore,the number of NGs in peripheral blood from the PE group was higher than that of the HP group;however,the levels of apoptosis were lower.The expression levels of miRNA-141-5p in NGs decreased,the expression of MAPK1 increased,and the secretion of neutrophil elastase in the NG medium increased in the PE group than those in the HP group.Conclusion:Collectively,our analysis suggested that miRNA-141-5p may be involved in the pathogenesis of PE by regulating the MAPK1 signaling pathway to activate neutrophils and increase the secretion of elastase. | Keyan Cheng Jialei Cui Wenli Zhou Huiqiang Liu Taotao Yang Yonghong Wang | 2022 | Maternal-Fetal Medicine2022，4，4： | 0 |
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