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| 1 | Design,methodology,and baseline of whole city‑million scale children and adolescents myopia survey(CAMS)in Wenzhou,China显示文摘Background:Myopia is the most common visual impairment in children and adolescents worldwide.This study described an economical and effective population-based screening pipeline and performed the project of a million scale children and adolescents myopia survey(CAMS),which will shed light on the further study of myopia from the level of epidemiology and precision medicine.Methods:We developed a novel population-based screening pattern,an intelligent screening process and internetbased information transmission and analysis system to carry out the survey consisting of school children in Wenzhou,China.The examination items include unaided distance visual acuity,presenting distance visual acuity,and noncycloplegic autorefraction.Myopia and high myopia were defined as spherical equivalent(SE)≤−1.00 diopters(D)and SE≤−6.00 D,respectively.Next,the reports of the vision checking were automatically sent to parents and the related departments.The CAMS project will be done two to four times annually with the support of the government.An online eyesight status information management system(OESIMS)was developed to construct comprehensive and efficient electronic vision health records(EVHRs)for myopia information inquiry,risk pre-warning,and further study.Results:The CAMS completed the first-round of screening within 30 days for 99.41%of Wenzhou students from districts and counties,in June 2019.A total of 1,060,925 participants were eligible for CAMS and 1,054,251(99.37%participation rate)were selected through data quality control,which comprised 1305 schools,and 580,609,251,050 and 170,967 elementary,middle,and high school students.The mean age of participants was 12.21±3.32 years(6-20 years),the female-to-male ratio was 0.82.The prevalence of myopia in elementary,middle,and high school students was 38.16%,77.52%,and 84.00%,respectively,and the high myopia incidence was 0.95%,6.90%,and 12.98%.Conclusions:The CAMS standardized myopia screening model involves automating large-scale information collection,data transmission,data analysis and early warning,thereby supporting myopia prevention and control.The entire survey reduced 90%of staff,cost,and time consumption compared with previous surveys.This will provide new insights for decision support for public health intervention. | Liangde Xu Youyuan Zhuang Guosi Zhang Yunlong Ma Jian Yuan Changseng Tu MiaoMiao Li Wencan Wang Yaru Zhang Xiaoyan Lu Jing Li Xinting Liu Zhengbo Xue Meng Zhou Jie Sun Jinhua Bao Ming Li Fan Lu Hong Wang Jianzhong Su Jia Qu | 2021 | Eye and Vision2021，8，1： | 7 |
| 2 | N-doped carbon nanocages: Bifunctional electrocatalysts for the oxygen reduction and evolution reactions显示文摘为氧进化反应(OER ) 和氧减小反应(ORR ) 的高度有效的没有金属的、基于碳的、双性人功能的 electrocatalysts 在电气化学的精力变换系统为使用吸引了增加的注意,由于他们的低费用和高活动。在这个工作,有多孔的 self-supported 体系结构和高特定的表面区域的做 N 的碳 nanocages (N-CCs ) 被一个灵巧的界面的集会综合合成线路。材料被扫描电子显微镜学,传播电子显微镜学,氮吸附解吸附作用实验, X 光检查衍射,和 X 光检查光电子光谱学包括地描绘。周期的 voltammetry, chronoamperometry,并且线性打扫 voltammetry 证明同样准备的 N-CC 能在碱的媒介为 ORR 与优秀催化活动,长期的操作耐久性,和优秀甲醇忍耐用作有效没有金属的 electrocatalyst。面对 3 公里甲醇,一半为 ORR 的 N-CCs 的波浪潜力是 190 mV;这比商业 Pt/C electrocatalyst 的更积极。同时, N-CCs 也显示出比得上商业 Ru/C electrocatalyst 的一项 OER 活动,揭示他们的 bifunctional 性质。 | Nan Jia Qiang Weng Yaru Shi Xinyan Shi Xinbing Chen Pei Chen Zhongwei An Yu Chen | 2018 | Nano Research2018，11，4： | 6 |
| 3 | Anti-Seizure and Neuronal Protective Effects of Irisin in Kainic Acid-Induced Chronic Epilepsy Model with Spontaneous Seizures显示文摘An increased level of reactive oxygen species is a key factor in neuronal apoptosis and epileptic seizures.Irisin reportedly attenuates the apoptosis and injury induced by oxidative stress.Therefore,we evaluated the effects of exogenous irisin in a kainic acid(KA)-induced chronic spontaneous epilepsy rat model.The results indicated that exogenous irisin significantly attenuated the KA-induced neuronal injury,learning and memory defects,and seizures.Irisin treatment also increased the levels of brain-derived neurotrophic factor(BDNF)and uncoupling protein 2(UCP2),which were initially reduced following KA administration.Furthermore,the specific inhibitor of UCP2(genipin)was administered to evaluate the possible protective mechanism of irisin.The reduced apoptosis,neurodegeneration,and spontaneous seizures in rats treated with irisin were significantly reversed by genipin administration.Our findings indicated that neuronal injury in KA-induced chronic epilepsy might be related to reduced levels of BDNF and UCP2.Moreover,our results confirmed the inhibition of neuronal injury and epileptic seizures by exogenous irisin.The protective effects of irisin may be mediated through the BDNF-mediated UCP2 level.Our results thus highlight irisin as a valuable therapeutic strategy against neuronal injury and epileptic seizures. | Jie Yu Yao Cheng Yaru Cui Yujie Zhai Wenshen Zhang Mengdi Zhang Wenyu Xin Jia Liang Xiaohong Pan Qiaoyun Wang Hongliu Sun | 2022 | Neuroscience Bulletin2022，38，11： | 1 |
| 4 | Surface display of carbonic anhydrase on Escherichia coli for CO_(2) capture and mineralization显示文摘Mineralization catalyzed by carbonic anhydrase(CA)is one of the most promising technologies for capturing CO_(2).In this work,Escherichia coli BL21(DE3)was used as the host,and the N-terminus of ice nucleation protein(INPN)was used as the carrier protein.Different fusion patterns and vectors were used to construct CA surface display systems forα-carbonic anhydrase(HPCA)from Helicobacter pylori 26695 andα-carbonic anhydrase(SazCA)from Sulfurihydrogenibium azorense.The surface display system in which HPCA was fused with INPN via a flexible linker and intermediate repeat sequences showed higher whole-cell enzyme activity,while the enzyme activity of the SazCA expression system was significantly higher than that of the HPCA expression system.The pET22b vector with the signal peptide PelB was more suitable for the cell surface display of SazCA.Cell frac-tionation and western-blot analysis indicated that SazCA and INPN were successfully anchored on the cell’s outer membrane as a fusion protein.The enzyme activity of the surface display strain E-22b-I RL S(11.43 U⋅mL^(−1) OD 600−1)was significantly higher than that of the intracellular expression strain E-22b-S(8.355 U⋅mL^(−1) OD 600−1)under optimized induction conditions.Compared with free SazCA,E-22b-I RL S had higher thermal and pH stability.The long-term stability of SazCA was also significantly improved by surface display.When the engineered strain and free enzyme were used for CO_(2) mineralization,the amount of CaCO_(3) deposition catalyzed by the strain E-22b-I RL S on the surface(241 mg)was similar to that of the free SazCA and was significantly higher than the intracellular expression strain E-22b-S(173 mg).These results demonstrate that the SazCA surface display strain can serve as a whole-cell biocatalyst for CO_(2) capture and mineralization. | Yinzhuang Zhu Yaru Liu Mingmei Ai Xiaoqiang Jia | 2022 | Synthetic and Systems Biotechnology2022，7，1： | 1 |
| 5 | Intraspecific and interspecific variation in Setaria revealed by RAPD analysis显示文摘 | Yu Li Jizeng Jia Yaru Wang Shuzhi Wu | 1998 | Genetic Resources and Crop Evolution1998，，3： | 1 |
| 6 | Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides显示文摘Anemarrhena asphodeloides is an immensely popular medicinal herb in China,which contains an abundant of mangiferin.As an important bioactive xanthone C-glycoside,mangiferin possesses a variety of pharmacological activities and is derived from the cyclization reaction of a benzophenone C-glycoside(maclurin).Biosyntheti-cally,C-glycosyltransferases are critical for the formation of benzophenone C-glycosides.However,the benzo-phenone C-glycosyltransferases from Anemarrhena asphodeloides have not been discovered.Herein,a promiscuous C-glycosyltransferase(AaCGT)was identified from Anemarrhena asphodeloides.It was able to catalyze efficiently mono-C-glycosylation of benzophenone,together with di-C-glycosylation of dihydrochalcone.It also exhibited the weak O-glycosylation or potent S-glycosylation capacities toward 12 other types of flavonoid scaffolds and a simple aromatic compound with–SH group.Homology modeling and mutagenesis experiments revealed that the glycosylation reaction of AaCGT was initiated by the conserved residue H23 as the catalytic base.Three critical residues H356,W359 and D380 were involved in the recognition of sugar donor through hydrogen-bonding interactions.In particular,the double mutant of F94W/L378M led to an unexpected enzy-matic conversion of mono-C-to di-C-glycosylation.This study highlights the important value of AaCGT as a potential biocatalyst for efficiently synthesizing high-value C-glycosides. | Jia Huang Yaru She Jingyang Yue Yidu Chen Yu Li Jing Li Yonger Hu Deying Yang Jiabo Chen Lu Yang Zhongqiu Liu Ruibo Wu Pengfei Jin Lixin Duan | 2022 | Synthetic and Systems Biotechnology2022，7，1： | 1 |
| 7 | A heteropolysaccharide from Rhodiola rosea L.:preparation,purification and anti-tumor activities in H22-bearing mice显示文摘Numerous polysaccharides isolated from plants have been used to augment traditional drugs in the treatment of cancer.In order to explore the influence to hepatocellular carcinoma,a novel cold water-soluble polysaccharide was separated from Rhodiola rosea L.root(RLP)and then its structure and anti-cancer activities were tested.The chemical compositions and high performance gel permeation chromatography(HPGPC)results indicated that RLP was an acid heteropolysaccharide with the molecular weight of about1.15×10~6 Da.Furthermore,ion chromatography(IC),Fourier transform infrared(FT-IR)and nuclear magnetic resoance(NMR)further indicated that RLP was main composed of→2,4)-α-Rha(1→,→5)-α-L-Araf-(1→,α-D-Glu,→6)-β-D-Galp-(1→,β-D-Man and→4)-α-GalpA-(1→.In vivo antitumor activities of RLP were carried out by using H22 tumor-bearing mice model.The results shown that RLP(100 and 300 mg/kg)could inhibit tumor growth of H22 cells from 23.59%to 45.52%and protect thymuses and spleen without damage.In addition,according to cell cycle,AV-FITC/PI and JC-1,RLP could induce dose-dependent apopto sis of H22 cells via S phase arrested which was through a mitochondrial related pathway.Our data indicated that RLP has a broader application prospect in anti-tumor preparations. | Yaru Wu Qing Wang Huiping Liu Lulu Niu Mengyu Li Qi Jia | 2023 | Food Science and Human Wellness2023，12，2： | 0 |
| 8 | Construction of AIEgen functionalized nanomicelles and their stability study through‘seesaw-like'fluorescence changes显示文摘As nanocarriers,nanomicelles play vital roles in the toolbox of drug delivery.The stability of nanomicelles affects the nanomedicines'bioactivity.Therefore,it is important to understand the stability of nanomicelles for further improvements.Here,we report a strategy to construct new nanomicelles(NM)by introducing aggregation-induced emission(AIE)functional group tetraphenylethylene(TPE)in the component polymer vitamin E(D-α-tocopheryl polyethylene glycol 1000 succinate)(TPGS).The stability of doxorubicin(DOX)loaded nanomicelles DOX@NM in different conditions was studied by fluorescence analysis.The fluorescence changes of DOX@NM are‘seesaw-like'when they transform between assembled and disassembled forms.In the assembled form,TPE gives emission from AIE effect,while in the disassembled form,the fluorescence of DOX is observed due to the disappearance of ACQ effect. | Xiaotong Wang Shizhu Chen Yaru Jia Kaihan Zhang Lili Ma Luwei Li Xingjie Liang Jinchao Zhang | 2023 | Chinese Chemical Letters2023，34，6： | 0 |
| 9 | Copper(Ⅱ) Acetylacetonate: An Efficient Catalyst for Huisgen- Click Reaction for Synthesis of 1,2,3-Triazoles in Water显示文摘 | Yuqin Jiang Xingfeng Li Xiyong Li Yamin Sun Yaru Zhao Shuhong Jia Niu Guo Guiqing Xu Weiwei Zhang | 2017 | Chinese Journal of Chemistry2017，35，8： | 0 |
| 10 | Effect of Ionic Concentration on Drug Release from Polyelectrolyte Hydrogel Carriers Analyzed via Triphasic Mechanism Model显示文摘 | LIU Yabo XU Yihan ZHAO Yaru JIA Yuxi | 2016 | Chemical Research in Chinese Universities2016，32，2： | 0 |
| 11 | The Yin and Yang of the protein corona on the delivery journey of nanoparticles显示文摘Nanoparticles-based drug delivery systems have attracted significant attention in biomedical fields because they can deliver loaded cargoes to the target site in a controlled manner.However,tremendous challenges must still be overcome to reach the expected targeting and therapeutic efficacy in vivo.These challenges mainly arise because the interaction between nanoparticles and biological systems is complex and dynamic and is influenced by the physicochemical properties of the nanoparticles and the heterogeneity of biological systems.Importantly,once the nanoparticles are injected into the blood,a protein corona will inevitably form on the surface.The protein corona creates a new biological identity which plays a vital role in mediating the bio–nano interaction and determining the ultimate results.Thus,it is essential to understand how the protein corona affects the delivery journey of nanoparticles in vivo and what we can do to exploit the protein corona for better delivery efficiency.In this review,we first summarize the fundamental impact of the protein corona on the delivery journey of nanoparticles.Next,we emphasize the strategies that have been developed for tailoring and exploiting the protein corona to improve the transportation behavior of nanoparticles in vivo.Finally,we highlight what we need to do as a next step towards better understanding and exploitation of the protein corona.We hope these insights into the“Yin and Yang”effect of the protein corona will have profound implications for understanding the role of the protein corona in a wide range of nanoparticles. | Yi-Feng Wang Yaxin Zhou JiaBei Sun Xiaotong Wang Yaru Jia Kun Ge Yan Yan Kenneth A.Dawson Shutao Guo Jinchao Zhang Xing-Jie Liang | 2023 | Nano Research2023，16，1： | 0 |
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