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| 1 | Parameterizing soil organic carbon's impacts on soil porosity and thermal parameters for Eastern Tibet grasslands显示文摘This study investigates the stratification of soil thermal properties induced by soil organic carbon (SOC) and its impacts on the parameterization of the thermal properties. Soil parameters were measured for alpine grassland stations and North China flux stations, with a total of 34 stations and 77 soil profiles. Measured data indicate that the topsoils of alpine grasslands contain high SOC contents than underlying soil layers, which leads to higher soil porosity values and lower thermal conductivity and bulk density values in the topsoils. However, this stratification is not evident at the lowland stations due to low SOC contents. Evaluations against measured data show that three thermal conductivity schemes used in land surface models severely overestimate the values for soils with high SOC content (i.e. topsoils of alpine grassland), but they are better for soils with low SOC content. A new parameterization is then developed to take the impacts of SOC into account. The new one can well estimate the soil thermal conductivity values in both low and high SOC content cases, and therefore, it is a potential candidate of thermal conductivity scheme to be used in land surface models. | CHEN YingYing YANG Kun TANG WenJun QIN Jun ZHAO Long | 2012 | Science China Earth Sciences2012，55，6： | 31 |
| 2 | Antidiabetic Effects of Gegen Qinlian Decoction via the Gut Microbiota Are Attributable to Its Key Ingredient Berberine显示文摘Gegen Qinlian Decoction(GQD),a traditional Chinese medicine(TCM)formula,has long been used for the treatment of common metabolic diseases,including type 2 diabetes mellitus.However,the main limitation of its wider application is ingredient complexity of this formula.Thus,it is critically important to identify the major active ingredients of GQD and to illustrate mechanisms underlying its action.Here,we compared the effects of GQD and berberine,a hypothetical key active pharmaceutical ingredient of GQD,on a diabetic rat model by comprehensive analyses of gut microbiota,short-chain fatty acids,proinflammatory cytokines,and ileum transcriptomics.Our results show that berberine and GQD had similar effects on lowering blood glucose levels,modulating gut microbiota,inducing ileal gene expression,as well as relieving systemic and local inflammation.As expected,both berberine and GQD treatment significantly altered the overall gut microbiota structure and enriched many butyrate-producing bacteria,including Faecalibacterium and Roseburia,thereby attenuating intestinal inflammation and lowering glucose.Levels of short-chain fatty acids in rat feces were also significantly elevated after treatment with berberine or GQD.Moreover,concentration of serum proinflammatory cytokines and expression of immune-related genes,including Nfkb1,Stat1,and Ifnrg1,in pancreatic islets were significantly reduced after treatment.Our study demonstrates that the main effects of GQD can be attributed to berberine via modulating gut microbiota.The strategy employed would facilitate further standardization and widespread application of TCM in many diseases. | Xizhan Xu Zezheng Gao Fuquan Yang Yingying Yang Liang Chen Lin Han Na Zhao Jiayue Xu Xinmiao Wang Yue Ma Lian Shu Xiaoxi Hu Na Lyu Yuanlong Pan Baoli Zhu Linhua Zhao Xiaolin Tong Jun Wang | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 29 |
| 3 | Three-stage aged refuse biofilter for the treatment of landfill leachate显示文摘A field-scale aged refuse(AR) biofilter constructed in Shanghai Refuse Landfill,containing about 7000 m3 aged refuse inside,was evaluated for its performance in the treatment of landfill leachate. This AR biofilter can be divided into three stages and can manage 50 m3 landfill leachate per day. The physical,chemical,and biological characteristics of AR were analyzed for evaluating the AR biofilter as leachate treatment host. The results revealed that over 87.8%-96.2% of COD and 96.9%-99.4% of ammonia nitrogen were removed by the three-stage AR biofilter when the influent leachate COD and ammonia nitrogen concentration were in the range 5478-10842 mg/L and 811-1582 mg/L,respectively. The final effiuent was inodorous and pale yellow with COD and ammonia nitrogen below 267-1020 mg/L and 6-45 mg/L,respectively. The three-stage AR biofilter had effcient nitrification but relative poor denitrification capacity with a total nitrogen(TN) removal of 58%-73%. The external temperature of AR biofilter did not influence the total ammonia nitrogen removal significantly. It was concluded that the scale-up AR biofilter can work very well and can be a promising technology for the treatment of landfill leachate. | LI Hongjiang ZHAO Youcai SHI Lei GU Yingying | 2009 | Journal of Environmental Sciences2009，21，1： | 11 |
| 4 | Ultrathin Al2O3-coated reduced graphene oxide membrane for stable lithium metal anode显示文摘Lithium(Li) metal has been considered as the most attractive anode materials for Li-ion batteries(LIBs)due to its high theoretic specific capacity. The formation of unstable solid electrolyte interphase(SEI) and dendritic Li on the metal anode, however, hindered its practical application. Herein, to address the issues, a Li-free electrode with ultrathin Al_2O_3 coated on reduced graphene oxide(rGO) membrane that covers a Cu foil current collector was developed. The composite electrode exhibits excellent interfacial protection of lithium metal deposited between Cu foil and rGO electrochemically. Firstly, it affords good Li^+ permeability from the electrolyte. Secondly, the ultrathin Al_2O_3 has sufficient mechanical strength to inhibit the penetration of Li dendrite. Li metal was observed uniformly deposited between rGO membrane and Cu collector, and stable cycle performance of Li plating/stripping with Coulombic efficiency of ~91.75% at the 100 th cycle is achieved in organic carbonate electrolyte without any additives. | Fan Zhang Fei Shen ZhaoYang Fan Xin Ji Bin Zhao ZhouTing Sun YingYing Xuan XiaoGang Han | 2018 | Rare Metals2018，37，6： | 11 |
| 5 | Myeloid-specific targeting of Notch ameliorates murine renal fibrosis via reduced infiltration and activation of bone marrowderived macrophage显示文摘Macrophages play critical roles in renal fibrosis.However,macrophages exhibit ontogenic and functional heterogeneities,and which population of macrophages contributes to renal fibrosis and the underlying mechanisms remain unclear.In this study,we genetically targeted Notch signaling by disrupting the transcription factor recombination signal binding protein-JK(RBP-J),to reveal its role in regulation of macrophages during the unilateral ureteral obstruction(UUO)-induced murine renal fibrosis.Myeloid-specific disruption of RBP-J attenuated renal fibrosis with reduced extracellular matrix deposition and myofibroblast activation,as well as attenuated epithelial-mesenchymal transition,likely owing to the reduced expression of TGF-β.Mean while,RBP-J deletion significantly hampered macrophage infiltration and activation in fibrotic kidney,although their proliferation appeared unaltered.By using macrophage clearance experiment,we found that kidney resident macrophages made negligible contribution,but bone marrow(BM)-derived macrophages played a major role in renal fibrogenesis.Further mechanistic analyses showed that Notch blockade reduced monocyte emigration from BM by down-regulating CCR2 expression.Finally,we found that myeloid-specific Notch activation aggravated renal fibrosis,which was mediated by CCR2^+macrophages infiltration.In summary,our data have unveiled that myeloid-specific targeting of Notch could ameliorate renal fibrosis by regulating BM-derived macrophages recruitment and activation,providing a novel strategy for intervention of this disease. | Yali Jiang Yuanyuan Wang Pengfei Ma Dongjie An Junlong Zhao Shiqian Liang Yuchen Ye Yingying Lu Peng Zhang Xiaowei Liu Hua Han Hongyan Qin | 2019 | Protein & Cell2019，10，3： | 10 |
| 6 | Development of a daily soil moisture product for the period of2002–2011 in Chinese mainland显示文摘Soil moisture is an essential climate variable(ECV) concerned widely. Due to its high spatial variability, it is costly to measure soil moisture at tens of kilometers scale. In this study, a ten-year(2002–2011) daily soil moisture dataset at 0.25° spatial resolution for Chinese mainland was produced through assimilating the Advanced Microwave Scanning Radiometer for Earth Observing System(AMSR-E) brightness temperature(TB) data into a land surface model(LSM). The obtained soil moisture data was evaluated against soil moisture-measuring networks deployed in two wet areas and one dry area of the Tibetan Plateau.The results show that for the wet areas the accuracy of the soil moisture product obtained from the assimilation is considerably higher than that of both AMSR-E official soil moisture products and land surface simulation results, and for the dry area their accuracy is comparable to each other. The spatial pattern of the soil moisture from the new product is consistent with that of soil porosity from an independent survey-based dataset, further confirming the credibility of the new product. According to this product, the transition regions in China show stronger seasonal variation of soil moisture than dry and wet regions, and drier regions have stronger inter-annual variability of soil moisture than wetter regions, particularly during transitional seasons(spring and autumn). The soil moisture product is accessible at the National Tibetan Plateau Data Center. | Kun YANG Yingying CHEN Jie HE Long ZHAO Hui LU Jun QIN Donghai ZHENG Xin LI | 2020 | Science China Earth Sciences2020，63，8： | 10 |
| 7 | Enantioselective separation and zebrafish embryo toxicity of insecticide beta-cypermethrin显示文摘Enantioselectivity of chiral pollutants is receiving growing concern due to the difference in toxicology and environment fate between enantiomers.In this study,enantiomers of insecticide beta-cypermethrin (beta-CP) were separated on selected chiral column by HPLC,and the toxicity of enantiomers was evaluated using the zebrafish embryo-larval assays.The enantiomers of beta-CP were baseline separated on Chiralcel OD and Chiralpak AD columns and detected by circular dichroism (CD) at 236 nm.Better separation could be achieved at lower temperature (e.g.,20°C) and with lower levels of polar modifiers.Pure enantiomers were obtained on Chiralcel OD.The CD spectra of enantiomers were recorded.By comparing the elution order with a previous similar study,the absolute configuration of beta-CP enantiomers was determined.The individual enantiomers were used in zebrafish embryo test,and the results showed that beta-CP enantioselectively induced yolk sac edema,pericardial edema and crooked body.The 1R-cis-αS and 1R-trans-αS enantiomers showed strong developmental toxicities at concentration of 0.1 mg/L,while the 1S-cis-αR and 1S-trans-αR induced no malformations at higher concentration (e.g.,0.3 mg/L).The results suggest that the enantioselective toxicological effects of beta-CP should be considered when evaluating its ecotoxicological effects. | Chao Xu,Wenqing Tu,Chun Lou,Yingying Hong,Meirong Zhao Research Center of Environmental Science,College of Biological and Environmental Engineering,Zhejiang University of Technology,Hangzhou 310032,China. | 2010 | Journal of Environmental Sciences2010，22，5： | 8 |
| 8 | Self-powered nanofiber-based screen-print triboelectric sensors for respiratory monitoring显示文摘科学、商业的进展将高要求放为便携电子学。然而,便携电子学的电源供应, breathability,和大量生产仍然有需要被克服的许多挑战。在这研究,一个自我动力的基于 nanofiber 的摩擦电传感器(SNTS ) 被批规模制造技术用 electrospinning 制作并且为经由呼吸监视监视的健康打印屏幕。典型地,一个拱门结构的 SNTS 被 nanofiber 膜和一个 Ag nanoparticle 电极装配。nanofibers 的堆积和 Ag nanoparticles 的传导性的网络越过整个设备保证一条煤气的隧道。当时, SNTS 的煤气的渗透象 6.16 mm/s 一样高,它有压到优势的优点与由密封演员组电影组成的通常使用的便携设备相比。由于 nanofiber 膜的柔软,不管它是否被弄弯,扭曲,或合拢, SNTS 显示出优秀电子输出性能。优异性质 SNTS 的皮友好,自我力量,和批制造例如 breathability,在保健监视和多功能的聪明的系统为他们的申请提供巨大的潜力。 | Ran Cao diaona Wang Shuyu Zhao Wei Yang Zuqing Yuan Yingying Yin Xinyu Du Nian-Wu Li Xiuling Zhang Xiuyan Li Zhong Lin Wang Congju Li | 2018 | Nano Research2018，11，7： | 8 |
| 9 | Single-cell analysis reveals bronchoalveolar epithelial dysfunction in COVID-19 patients显示文摘Dear Editor,In 2019,a zoonotic coronavirus named severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)was identified as the causative agent of Coronavirus Disease 2019(COVID-19).As of 8 June 2020,the World Health Organization(WHO)has reported 6,912,751 globally confirmed cases with 400,469 deaths.Although generally causes mild disease,SARS-CoV-2 infection can result in serious outcomes,including acute lung injury(ALI)and acute respiratory distress syndrome(ARDS),the leading cause of mortality in patients with comorbidities.Recent autopsy studies of COVID-19 patients revealed mononuclear infiltration and excessive production of mucus in the infected lung,especially in the damaged small airways and alveoli(Bian and Team,2020;Liu et al.,2020). | Jiangping He Shuijiang Cai Huijian Feng Baomei Cai Lihui Lin Yuanbang Mai Yinqiang Fan Airu Zhu Huang Huang Junjie Shi Dingxin Li' Yuanjie Wei Yueping Li Yingying Zhao’ Yuejun Pan He Liu Xiaoneng Mo Xi He Shangtao Cao FengYu Hu Jincun Zhao Jie Wang Nanshan Zhong Xinwen Chen Xilong Deng Jiekai Chen | 2020 | Protein & Cell2020，11，9： | 7 |
| 10 | Two-dimensional ultrathin MoS2-modified black Ti^3+-TiO2 nanotubes for enhanced photocatalytic water splitting hydrogen production显示文摘Two-dimensional (2D) ultrathin MoS2-modified black Ti^3+-TiO2 nanotubes were fabricated using an electrospinning-hydrothermal treatment-reduction method.Bare TiO2 nanotubes were fabricated via electrospinning.Then,2D MoS2 lamellae were grown on the surface of the nanotubes and Ti^3+/Ov ions were introduced by reduction.The photocatalytic performance of the 2D MoS2/Ti^3+-TiO2 nanotubes was^15 times better than that of TiO2.The HER enhancement of the MoS2/Ti^3+-TiO2 nanotubes can be attributed to the Pt-like behavior of 2D MoS2 and the presence of Ti^3+-ions,which facilitated the quick diffusion of the photogenerated electrons to water,reducing the H2 activation barrier.The presence of Ov ions in the nanotubes and their hollow structure increased their solar utilization. | Wei Ou Jiaqi Pan Yanyan Liu Shi Li Hongli Li Weijie Zhao Jingjing Wang Changsheng Song Yingying Zheng Chaorong Li | 2020 | Journal of Energy Chemistry2020，29，4： | 7 |
| 11 | A Comparison Study of Working Memory Deficits between Patients with Methamphetamine-Associated Psychosis and Patients with Schizophrenia显示文摘 | Hong GAN Zhenhua SONG Peiwei XU Hang SU Yingying PAN Min ZHAO Dengtang LIU | 2018 | 上海精神医学2018，30，3： | 6 |
| 12 | Characterization and mapping of QTLs on chromosome 2D for grain size and yield traits using a mutant line induced by EMS in wheat显示文摘Production of mutants with altered phenotypes is a powerful approach for determining the biological functions of genes in an organism. In this study, a high-grain-weight mutant line M8008 was identified from a library of mutants of the common wheat cultivar YN15 treated with ethylmethane sulfonate(EMS). F2 and F2:3generations produced from crosses of M8008 × YN15(MY) and M8008 × SJZ54(MS) were used for genetic analysis. There were significant differences between M8008 and YN15 in plant height(PH), spike length(SL),fertile spikelet number per spike(FSS), grain width(GW), grain length(GL), GL/GW ratio(GLW), and thousand-grain weight(TGW). Most simple correlation coefficients were significant for the investigated traits, suggesting that the correlative mutations occurred in M8008. Approximately 21% of simple sequence repeat(SSR) markers showed polymorphisms between M8008 and YN15, indicating that EMS can induce a large number of mutated loci. Twelve quantitative trait loci(QTLs) forming QTL clusters(one in MY and two in MS) were detected. The QTL clusters coinciding with(MY population) or near(MS population) the marker wmc41 were associated mainly with grain-size traits, among which the M8008 locus led to decreases in GW, factor form density(FFD), and TGW and to increases in GLW. The cluster in the wmc25–barc168 interval in the MS population was associated with yield traits, for which the M8008 locus led to decreased PH, spike number per plant(SN), and SL. | Guizhi Zhang Yingying Wang Ying Guo Yan Zhao Fanmei Kong Sishen Li | 2015 | The Crop Journal2015，3，2： | 6 |
| 13 | Leachate treatment using a demonstration aged refuse biofilter显示文摘Approximately 7000 m3 of aged refuse(AR) with a placement of over eight years was excavated from Shanghai Refuse Landfill,the largest landfill in China,and used for the construction of a two-stage bioreactor(AR biofilter) media for the biological treatment of 100 m3 of refuse landfill leachate.It was found that over 64% of COD,96.9%-99.8% of NH4+-N,and 95.8%-99.8% of BOD5 could be removed by the AR biofilter,when the leachate with initial COD,BOD5,and NH4+-N concentrations were 986-4128 mg/L,264-959 mg/L,and 538-1583 mg/L,respectively.The corresponding concentrations in the effluent were reduced to below 300-400 mg/L,2-12 mg/L,and 10-20 mg/L,respectively.The effluent was clear and pale yellow with suspended solid below 150 mg/L and color below 150 Pt/Co degree.Meanwhile,the total nitrogen removal was only 49%-63%,indicating a relative poor denitrification capacity of AR biofilter.The effluent pH was neutral and the population of Escherichia coli was less than 10-1 CFU/mL.Hence,it was considered that the demonstration project can work well for the effective treatment of leachate. | Hongjiang Li Yingying Gu Youcai Zhao Zhiping Wen | 2010 | Journal of Environmental Sciences2010，22，7： | 6 |
| 14 | Gas Sensors Based on Chemi?Resistive Hybrid Functional Nanomaterials显示文摘Chemi-resistive sensors based on hybrid functional materials are promising candidates for gas sensing with high responsivity,good selectivity,fast response/recovery,great stability/repeatability,room-working temperature,low cost,and easy-to-fabricate,for versatile applications.This progress report reviews the advantages and advances of these sensing structures compared with the single constituent,according to five main sensing forms:manipulating/constructing heterojunctions,catalytic reaction,charge transfer,charge carrier transport,molecular binding/sieving,and their combinations.Promises and challenges of the advances of each form are presented and discussed.Critical thinking and ideas regarding the orientation of the development of hybrid material-based gas sensor in the future are discussed. | Yingying Jian Wenwen Hu Zhenhuan Zhao Pengfei Cheng Hossam Haick Mingshui Yao Weiwei Wu | 2020 | Nano-Micro Letters2020，12，6： | 5 |
| 15 | Effects of Cu^(2+)and humic acids on degradation and fate of TBBPA in pure culture of Pseudomonas sp.strain CDT显示文摘Soil contamination with tetrabromobisphenol A(TBBPA) has caused great concerns;however, the presence of heavy metals and soil organic matter on the biodegradation of TBBPA is still unclear. We isolated Pseudomonas sp. strain CDT, a TBBPA-degrading bacterium, from activated sludge and incubated it with ^(14)C-labeled TBBPA for 87 days in the absence and presence of Cu^(2+)and humic acids(HA). TBBPA was degraded to organic-solvent extractable(59.4% ± 2.2%) and non-extractable(25.1% ± 1.3%) metabolites,mineralized to CO_2(4.8% ± 0.8%), and assimilated into cells(10.6% ± 0.9%) at the end of incubation. When Cu^(2+)was present, the transformation of extractable metabolites into non-extractable metabolites and mineralization were inhibited, possibly due to the toxicity of Cu^(2+)to cells. HA significantly inhibited both dissipation and mineralization of TBBPA and altered the fate of TBBPA in the culture by formation of HA-bound residues that amounted to 22.1% ± 3.7% of the transformed TBBPA. The inhibition from HA was attributed to adsorption of TBBPA and formation of bound residues with HA via reaction of reactive metabolites with HA molecules, which decreased bioavailability of TBBPA and metabolites in the culture. When Cu^(2+)and HA were both present, Cu^(2+)significantly promoted the HA inhibition on TBBPA dissipation but not on metabolite degradation. The results provide insights into individual and interactive effects of Cu^(2+)and soil organic matter on the biotransformation of TBBPA and indicate that soil organic matter plays an essential role in determining the fate of organic pollutants in soil and mitigating heavy metal toxicity. | Yini Ma Yingying Zhao Yongfeng Wang Xiangzhen Li Feifei Sun Phillippe Francois-Xavier Corvini Rong Ji | 2017 | Journal of Environmental Sciences2017，29，12： | 5 |
| 16 | A CIB1-LIKE transcription factor GmC IL10 from soybean positively regulates plant flowering显示文摘CRYPTOCHROME-INTERACTING basic helix-loop-helix 1(CIB1) is a well characterized transcriptional factor which promotes flowering through the physical interaction with the blue light receptor CRYPTOCHROME 2(CRY2) in Arabidopsis. However, the role of its counterpart in crop species remains largely unknown. Here, we describe the isolation and characterization of a CIB1 homolog gene, Glycine max CIB1-LIKE10(GmC IL10), from soybean genome. The m RNA expression of GmC IL10 in the unifoliate leaves shows a diunal rhythm in both long day(LD) and short day(SD) photoperiod, but it only oscillates with a circadian rhythm when the soybean is grown under LDs, indicating that the clock regulation of GmC IL10 transcription is LD photoperiod-dependent. Moreover, its m RNA expression varies in different tissue or organs, influenced by the develpomental stage, implying that GmC IL10 may be involved in the regulation of multiple developmental processes. Similar to CIB1, Gm CIL10 was evident to be a nuclei protein and ectopically expression of GmC IL10 in transgenic Arabidopsis accelerates flowering under both LDs and SDs, implying that CIBs dependent regulation of flowering time is an evolutionarily conserved mechanism in different plant species. | YANG DeGuang ZHAO Wang MENG YingYing LI HongYu LIU Bin | 2015 | Science China(Life Sciences)2015，58，3： | 4 |
| 17 | 152 W high-power blue diode laser operated at 447 nm显示文摘We demonstrate a high-power blue diode laser operated at 447 nm combining laser diodes using an optical fiber bundle. As many as 127 diode lasers at 447 nm were coupled into 400 μm/0.22 NA fibers using an aspherical lens group with different focus lengths. The bare fibers were mechanically bundled through high temperature ultraviolet adhesive after the coatings of the 127 fibers were stripped. The diameter of the fiber bundle was 6 mm. The total output power of such a bundle was 152 W with electro-optical conversion efficiency of 27.56%and the RMS power instability was less than ±1% within 3 h. | Peng Wu Ling Zhang Haijuan Yu Xiandan Yuan Zhiyan Zhang Pengfei Zhao Shuzhen Zou Chaojian He Yaoyao Qi Yingying Yang Gang Li Xubao Wang Xuechun Lin | 2017 | Journal of Semiconductors2017，38，7： | 4 |
| 18 | Dual versus mono antiplatelet therapy for acute non-cardioembolic ischaemic stroke or transient ischaemic attack:a systematic review and meta-analysis显示文摘Objective Recent years have seen new evidence on the efficacy and safety of dual antiplatelet therapy for secondary stroke prevention.We updated a meta-analysis of randomised controlled trials evaluating dual antiplatelet versus monotherapy for patients with acute non-cardioembolic ischaemic stroke(IS)or transient ischaemic attack(TIA).Methods We searched PubMed and identified randomised controlled trials evaluating dual antiplatelet versus monotherapy for acute non-cardioembolic IS or TIA within 3 days of ictus up to May 2018.Risk ratio(RR)with 95%CI were calculated using random effects models.Clinical endpoints included stroke recurrence,composite vascular events and major bleeding.results 18 randomised controlled trials including 15515 patients were pooled in the meta-analysis.When compared with monotherapy among patients with acute IS or TIA,dual antiplatelet therapy reduced the risk of stroke recurrence(RR 0.69;95%CI 0.61 to 0.78;p<0.001)and composite vascular events(RR 0.72;95%CI 0.64 to 0.80;p<0.001).Dual therapy was associated with a significant increase in the risk of major bleeding(RR 1.77;95%CI 1.09 to 2.87;p=0.02)when all trial data were combined.However,when all previous trials before the completion of the POINT trial were analysed,dual antiplatelet versus monotherapy was not associated with a significant increase in the risk of major bleeding(RR 1.46;95%CI 0.77 to 2.75;p=0.25).Conclusions Among patients with acute noncardioembolic IS or TIA within 3 days of ictus,dual antiplatelet therapy was associated with a reduction in stroke recurrence,and composite vascular events,when compared with monotherapy.However,a significant increase in the risk of major bleeding was observed. | Yingying Yang Mengyuan Zhou Xi Zhong Yongjun Wang Xingquan Zhao Liping Liu Yilong Wang | 2018 | Stroke & Vascular Neurology2018，3，2： | 4 |
| 19 | Efficacy of Lidan Tang on high-fat-diet induced gallstone in mice and possible mechanism显示文摘OBJECTIVE:To investigate efficacy of Lidan Tang(LDT)on gallstone induced by high fat diet in mice,and to study its underlying mechanism.METHODS:Mice were fed with high fat diet every day and treated with LDT(9.01 times of human clinic dosage).Mice were randomly divided into 6 groups as control group,gallstone model group(high-fat diet),positive control ursodeoxycholic acid(UDCA)group(80 mg·kg^-1·d^-1,i.g.),LDT low dose group(6 kg/d,i.g.),LDT middle dose group(12 kg/d,i.g.),and LDT high dose group(24 kg/d,i.g.).The whole experiment was lasted for 4 weeks.The levels of ALT,AST,LDH,CHO,HDL-C and LDL-C in serum were measured,the pathological sections were observed by hematoxylin-eosin staining,the activities of antioxidant enzymes were measured by kits,and the proteins related to oxidative stress and lipid transport were detected by Western blot analysis.RESULTS:LDT could significantly reduce the contents of ALT and AST in serum and improve the pathological tissue of liver.LDT could significantly reduce the content of MDA and LPO,and increase the level of GSH and GSH-PX in liver tissue.The data of Western blot showed that LDT had antioxidant effect promoting Keap1/Nrf2 pathway and regulated the process of lipid transport,which was statistically significant.In addition,LDT treatment inhibited the expression of ATP-binding cassette transports ABCG5/8 in liver,and reduced cholesterol transport from the hepatocytes to the gallbladder.CONCLUSION:LDT has protective effect on gallstones induced by high fat diet in mice,which might be based on the protective effect on liver,including enhancing the antioxidant capacity of liver and reducing the production of lipid peroxides. | Gao Yan Liu Caipin Li Juntong Zhai Yingying Lin Meiyu Wu Qinglin Chu Shifeng Zhang Zhao Li Jianping Zhou Xin Li Yueting Chen Naihong | 2020 | Journal of Traditional Chinese Medicine2020，40，4： | 4 |
| 20 | Structural basis for bivalent binding and inhibition of SARS-CoV-2 infection by human potent neutralizing antibodies显示文摘Neutralizing monoclonal antibodies(nAbs)to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)represent promising candidates for clinical intervention against coronavirus disease 2019(COVID-19).We isolated a large number of nAbs from SARS-CoV-2-infected individuals capable of disrupting proper interaction between the receptor binding domain(RBD)of the viral spike(S)protein and the receptor angiotensin converting enzyme 2(ACE2).However,the structural basis for their potent neutralizing activity remains unclear.Here,we report cryo-EM structures of the ten most potent nAbs in their native full-length IgG-form or in both IgG-form and Fab-form bound to the trimeric S protein of SARS-CoV-2.The bivalent binding of the full-length IgG is found to associate with more RBDs in the“up”conformation than the monovalent binding of Fab,perhaps contributing to the enhanced neutralizing activity of IgG and triggering more shedding of the S1 subunit from the S protein.Comparison of a large number of nAbs identified common and unique structural features associated with their potent neutralizing activities.This work provides a structural basis for further understanding the mechanism of nAbs,especially through revealing the bivalent binding and its correlation with more potent neutralization and the shedding of S1 subunit. | Renhong Yan Ruoke Wang Bin Ju Jinfang Yu Yuanyuan Zhang Nan Liu Jia Wang Qi Zhang Peng Chen Bing Zhou Yaning Li Yaping Shen Shuyuan Zhang Long Tian Yingying Guo Lu Xia Xinyue Zhong Lin Cheng Xiangyang Ge Juanjuan Zhao Hong-Wei Wang Xinquan Wang Zheng Zhang Linqi Zhang Qiang Zhou | 2021 | Cell Research2021，31，5： | 4 |
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