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| 1 | The adult human testis transcriptional cell atlas显示文摘Human adult spermatogenesis balances spermatogonial stem cell (SSC) self-renewal and differentiation,alongside complex germ cell-niche interactions,to ensure long-term fertility and faithful genome propagation. Here,we performed single-cell RNA sequencing of -6500 testicular cells from young adults. We found five niche/somatic cell types (Leydig,myoid,Sertoli,endothelial,macrophage),and observed germline-niche interactions and key human-mouse differences. Spermatogenesis,including meiosis,was reconstructed computationally,revealing sequential coding,non-coding,and repeat-element transcriptional signatures. Interestingly,we identified five discrete transcriptional/developmental spermatogonial states,including a novel early SSC state,termed State 0. Epigenetic features and nascent transcription analyses suggested developmental plasticity within spermatogonial States. To understand the or丨gin of State 0,we profiled testicular cells from infants,and identified distinct similarities between adult State 0 and infant SSCs. Overall,our datasets describe key transcriptional and epigenetic signatures of the normal adult human testis,and provide new insights into germ cell developmental transitions and plasticity. | Jingtao Guo Edward J. Grow Hana Mlcochova Geoffrey J. Maher Cecilia Lindskog Xichen Nie Yixuan Guo Yodai Takei Jina Yun Long Cai Robin Kim Douglas T. Carrell Anne Goriely James M. Hotaling Bradley R. Cairns | 2018 | Cell Research2018，28，12： | 12 |
| 2 | Effects of laser scanning strategies on selective laser melting of pure tungsten显示文摘Three types of scanning strategies,including the chessboard scanning strategy,the zigzag scanning strategy and the remelting scanning strategy,were conducted to study the effects of scanning strategies on surface morphology,microstructure,mechanical properties and the grain orientation of selective laser melted pure tungsten.The results showed that the pores and cracks were main defects in SLM-processed tungsten parts.The pores could be eliminated using the remelting scanning strategy.However,the cracks seemed to be inevitable regardless of the applied scanning strategies.The microstructures of SLM-processed tungsten were columnar grains and showed strong epitaxial growth along the building direction.A compressive strength of 923 MPa with an elongation of 7.7%was obtained when the zigzag scanning strategy was applied,which was the highest among the three scanning strategies.By changing the scanning strategies,the texture of SLM-processed tungsten in the direction of processing could be changed. | Dongdong Gu Meng Guo Hongmei Zhang Yixuan Sun Rui Wang Lei Zhang | 2020 | International Journal of Extreme Manufacturing2020，2，2： | 6 |
| 3 | Precise temporal regulation of Dux is important for embryo development显示文摘Dear Editor,Zygotic genome activation(ZGA)following fertilization is accomplished through a process termed the maternal-to-zygotic transition,during which the maternal RNAs and proteins are degraded and zygotic genome is transcriptionally activated.In mice,minor ZGA occurs from S phase of the zygote to G1 phase of the two-cell(2C)embryo,while major ZGA takes place during the middle-to-late 2C stage with a burst of transcription of totipotent cleavage stage-specific genes and retrotransposons. | Mingyue Guo Yanping Zhang Jianfeng Zhou Yan Bi Junqin Xu Ce Xu Xiaochen Kou Yanhong Zhao Yanhe Li Zhifen Tu Kuisheng Liu Jiaming Lin Peng Yang Shaorong Gao Yixuan Wang | 2019 | Cell Research2019，29，11： | 5 |
| 4 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 5 | Probabilistic forecasting based on ensemble forecasts and EMOS method for TGR inflow显示文摘Probabilistic inflow forecasts can quantify the uncertainty involved in the forecasting process and provide useful risk information for reservoir management.This study proposed a probabilistic inflow forecasting scheme for the Three Gorges Reservoir(TGR)at 1-3 d lead times.The post-processing method Ensemble Model Output Statistics(EMOS)is used to derive probabilistic inflow forecasts from ensemble inflow forecasts.Considering the inherent skew feature of the inflow series,lognormal and gamma distributions are used as EMOS predictive distributions in addition to conventional normal distribution.Results show that TGR's ensemble inflow forecasts at 1-3 d lead times perform well with high model efficiency and small mean absolute error.Underestimation of forecasting uncertainty is observed for the raw ensemble inflow forecasts with biased probability integral transform(PIT)histograms.The three EMOS probabilistic forecasts outperform the raw ensemble forecasts in terms of both deterministic and probabilistic performance at 1-3 d lead times.The EMOS results are more reliable with much flatter PIT histograms,coverage rates approximate to the nominal coverage 89.47%and satisfactory sharpness.Results also show that EMOS with gamma distribution is superior to normal and lognormal distributions.This research can provide reliable probabilistic inflow forecasts without much variation of TGR5s operational inflow forecasting procedure. | Yixuan ZHONG Shenglian GUO Feng XIONG Dedi LIU Huanhuan BA Xushu WU | 2020 | Frontiers of Earth Science2020，14，1： | 3 |
| 6 | Targeted core-shell nanoparticles for precise CTCF gene insert in treatment of metastatic breast cancer显示文摘Clustered regularly interspaced short palindromic repeats(CRISPR)technology emerges a remarkable potential for cure of refractory cancer like metastatic breast cancer.However,how to efficiently deliver the CRISPR system with non-viral carrier remains a major issue to be solved.Here,we report a kind of targeted core-shell nanoparticles(NPs)carrying dual plasmids(pHR-pCas9)for precise CCCTC-binding factor(CTCF)gene insert to circumvent metastatic breast cancer.The targeted core-shell NPs carrying pHR-pCas9 can accomplishγGTP-mediated cellular uptake and endosomal escape,facilitate the precise insert and stable expression of CTCF gene,inhibit the migration,metastasis,and colonization of metastatic breast cancer cells.Besides,the finding further reveals that the inhibitory mechanism of metastasis could be associated with up-regulating CTCF protein,followed by down-regulating stomatin(STOM)protein.The study offers a universal nanostrategy enabling the robust non-viral delivery of gene-editing system for treatment of severe illness. | Jialun Duan Chunjie Bao Ying Xie Haitao Guo Yixuan Liu Jianwei Li Rui Liu Peishan Li Jing Bai Yan Yan Limin Mu Xueqi Li Guiling Wang Wanliang Lu | 2022 | Bioactive Materials2022，7，5： | 1 |
| 7 | Potassium pre-inserted K1.04Mn8O16 as cathode materials for aqueous Li-ion and Na-ion hybrid capacitors显示文摘For the applications of aqueous Li-ion hybrid capacitors and Na-ion hybrid capacitors,potassium ions are pre-inserted into MnO2 tunnel structure,the as-prepared K1.04Mn8 O16 materials consist of nanoparticles and nanorods were prepared by facile high-temperature solid-state reaction.The as-prepared materials were well studied and they show outstanding electrochemical behavior.We assembled hybrid supercapacitors with commercial activated carbon(YEC-8 A)as anode and K1.04Mn8 O16 as cathode.It shows high energy and power densities.Li-ion capacitors reach a high energy density of 127.61 Wh kg-1 at the power density of 99.86 W kg-1 and Na-ion capacitor obtains 170.96 Wh kg-1 at 133.79 W kg-1.In addition,the hybrid supercapacitors demonstrate excellent cycling performance which maintain 97%capacitance retention for Li-ion capacitor and 85%for Na-ion capacitor after 10,000 cycles. | Yamin Zhang Lina Chen Chongyang Hao Xiaowen Zheng Yixuan Guo Long Chen Kangrong Lai Yinghe Zhang Lijie Ci | 2020 | Journal of Energy Chemistry2020，29，7： | 1 |
| 8 | Research on dynamics simulation of CNC milling machine based on virtual prototyping 显示文摘 | WANG Yixuan GUO Yanli WANG Ying | 2013 | International Review of Mechanical Engineering2013，7，5： | 1 |
| 9 | RNA-Seq analysis reveals the growth and photosynthetic responses of rapeseed(Brassica napus L.)under red and blue LEDs with supplemental yellow,green,or white light显示文摘Compound light is required for plant growth and development,but the response mechanisms of plants are undercharacterized and not fully understood.The present study was undertaken to evaluate the effect of supplemental light(green light,G;white light,W;yellow light,Y)added to red–blue light(RB)and sole W on the growth and photosynthesis of rapeseed seedlings.The results revealed that supplemental G/W improved the growth and photosynthesis of seedlings,but supplemental Y significantly reduced the photosynthetic rate and palisade tissue layer.Sole W caused similar responses in terms of growth,leaf development,oxidative damage,and antioxidant capability as supplemental Y.In total,449,367,813,and 751 differentially expressed genes(DEGs)were identified under supplemental G,Y,and W and sole W,respectively,compared to RB.The DEGs under different lights were closely associated with pathways such as light stimulus and high-light response,root growth,leaf development,photosynthesis,photosynthesis-antenna proteins,carbohydrate synthesis and degradation,secondary metabolism,plant hormones,and antioxidant capacity,which contributed to the distinct growth and photosynthesis under different treatments.Our results suggest that Y is more likely substituted by other wavelengths to achieve certain effects similar to those of supplemental Y,while G has a more distinctive effect on rapeseed.Taken together,supplementation RB with G/W promotes the growth of rapeseed seedlings in a controlled environment. | Xiaoying Liu Zheng Chen Mohammad Shah Jahan Yixuan Wen Xuyang Yao Haifeng Ding Shirong Guo Zhigang Xu | 2020 | Horticulture Research2020，7，1： | 1 |
| 10 | Cow dung-derived biochars engineered as antibacterial agents for bacterial decontamination显示文摘Disposal of the pollutants arising from farming cattle and other livestock threatens the environment and public safety in diverse ways.Herein,we report on the synthesis of engineered biochars using cow dung as raw material,and investigating these biochars as antibacterial agents for water decontamination.By coating the biochars with N-halamine polymer and loading them with active chlorine (i.e.,Cl+),we were able to regulate them on demand by tuning the polymer coating and bleaching conditions.The obtained N-halamine-modified biochars were found to be extremely potent against Escherichia coli and Staphylococcus aureus.We also investigated the possibility of using these N-halamine-modified biochars for bacterial decontamination in real-world applications.Our findings indicated that a homemade filter column packed with N-halamine-modified biochars removed pathogenic bacteria from mining sewage,dairy sewage,domestic sewage,and artificial seawater.This proposed strategy could indicate a new way for utilizing livestock pollutants to create on-demand decontaminants. | Quanfu Yao Qinggele Borjihan Huihui Qu Yixuan Guo Ziying Zhao Long Qiao Ting Li Alideertu Dong Ying Liu | 2021 | Journal of Environmental Sciences2021，33，7： | 1 |
| 11 | Color Scheme of Shoes Equally Requires 'People Oriented'显示文摘The basic principle that shoes would indicate its maximum value only when they are worn by people is exactly what people constantly ignore.How to find out the recipe toones own color matching?The key answer would be'people oriented'. | Guo Yixuan | 2013 | China Textile2013，，3： | 0 |
| 12 | Mild and efficient recovery of lithium-ion battery cathode material by deep eutectic solvents with natural and cheap components显示文摘Dissolution of lithium cobalt oxide(LCO)is the key step for the recovery of valuable metals(e.g.,Co and Li)from spent LCO-based lithium-ion batteries(LIBs).However,the dissolution process of LCO either needs toxic solvents,and high temperature,or shows low efficiency.Deep eutectic solvents(DESs)are potential green solvents to dissolve LCO.Here,DESs with polyethylene glycol(PEG)as hydrogen bond acceptor and ascorbic acid(AA)as hydrogen bond donor are found to dissolve LCO with 84.2%Co leaching efficiency at 80℃ and 72 h,which is higher than that from the reported references by common DESs.Furthermore,both DESs components(i.e.,PEG and AA)are cheap,biodegradable,and biocompatible.AA could be easily and abundantly extracted from natural fruits or vegetables.It provides a new guide for the green,mild,and efficient dissolution of LCO aiming at sustainable recovery of spent LIBs. | Yu Chen Yanlong Wang Yue Bai Minghui Feng Fengyi Zhou Yanhong Lu Yuting Guo Yixuan Zhang Tiancheng Mu | 2023 | Green Chemical Engineering2023，4，3： | 0 |
| 13 | Flexible assistance strategy of lower limb rehabilitation exoskeleton based on admittance model显示文摘Exercise training based on exoskeleton is an effective rehabilitation method for stroke patients. However, some rehabilitation exoskeletons still have poor wearable performance and obvious human-robot impedance during training, which easily cause secondary injuries to the patients. In this study, a variable admittance control strategy is proposed to improve the operator's wearable comfort, which can adapt to different operators by regulating admittance parameters. The admittance controller has two feedback loops: the position inner-loop and the admittance outer-loop. Meanwhile, the exoskeleton Lagrange model is constructed with unknown friction disturbance. By using Lyapunov technique and backstepping with state observer, the joint position error of exoskeleton is convergence into a zero neighborhood. The effectiveness of the proposed admittance controller is verified by both simulation and experiment. The tracking error of hip and knee joint is less than 4 degrees while the human-robot interaction torque is constrained in a tolerable range of the operator. | KOU JianGe WANG YiXuan CHEN ZhenLei SHI Yan GUO Qing XU Meng | 2024 | Science China(Technological Sciences)2024，67，3： | 0 |
| 14 | Key Optimization Issues for Renewable Energy Systems under Carbon-Peaking and Carbon Neutrality Targets:Current States and Perspectives显示文摘1 Introduction The United States,Japan,Canada,the European Union,and other developed countries and regions have all formulated climate strategies and pledged to achieve net-zero CO_(2) emissions by 2050.China,meanwhile,has announced through the“carbon-peaking and carbon neutrality targets”in September 2020 that it aims to achieve“peak carbon use”by 2030 and“carbon neutrality”by 2060[1].According to statistical data from the International Energy Agency(IEA),Fig.1 illustrates the carbon intensity of electricity generation in various regions in the Announced Pledge Scenario(APS)from 2010 to 2040[2].One can easily observe that each region aims to accomplish a sharp decrease in the carbon intensity of electricity generation after 2020. | Bo Yang Zhengxun Guo JingboWang Chao Duan Yaxing Ren Yixuan Chen | 2022 | Energy Engineering2022，119，5： | 0 |
| 15 | Vote-Based Feature Selection Method for Stratigraphic Recognition in Tunnelling Process of Shield Machine显示文摘Shield machines are currently the main tool for underground tunnel construction. Due to the complexity and variability of the underground construction environment, it is necessary to accurately identify the ground in real-time during the tunnel construction process to match and adjust the tunnel parameters according to the geological conditions to ensure construction safety. Compared with the traditional method of stratum identifcation based on staged drilling sampling, the real-time stratum identifcation method based on construction data has the advantages of low cost and high precision. Due to the huge amount of sensor data of the ultra-large diameter mud-water balance shield machine, in order to balance the identifcation time and recognition accuracy of the formation, it is necessary to screen the multivariate data features collected by hundreds of sensors. In response to this problem, this paper proposes a voting-based feature extraction method (VFS), which integrates multiple feature extraction algorithms FSM, and the frequency of each feature in all feature extraction algorithms is the basis for voting. At the same time, in order to verify the wide applicability of the method, several commonly used classifcation models are used to train and test the obtained efective feature data, and the model accuracy and recognition time are used as evaluation indicators, and the classifcation with the best combination with VFS is obtained. The experimental results of shield machine data of 6 diferent geological structures show that the average accuracy of 13 features obtained by VFS combined with diferent classifcation algorithms is 91%;among them, the random forest model takes less time and has the highest recognition accuracy, reaching 93%, showing best compatibility with VFS. Therefore, the VFS algorithm proposed in this paper has high reliability and wide applicability for stratum identifcation in the process of tunnel construction, and can be matched with a variety of classifer algorithms. By combining 13 features selected from shield machine data features with random forest, the identifcation of the construction stratum environment of shield tunnels can be well realized, and further theoretical guidance for underground engineering construction can be provided. | Liman Yang Xuze Guo Jianfu Chen Yixuan Wang Huaixiang Ma Yunhua Li Zhiguo Yang Yan Shi | 2023 | Chinese Journal of Mechanical Engineering2023，36，5： | 0 |
| 16 | Immunosuppressive therapy selectively modulates B-cell responses in patients with rheumatic and musculoskeletal diseases receiving the inactivated COVID-19 vaccine显示文摘Background:Immunosuppressive medication reduces the immunogenicity of the coronavirus disease 2019(COVID-19)vaccines in patients with rheumatic and musculoskeletal diseases(RMDs).However,the underlying mechanism remains unclear.The primary aim of our study was to dissect the impact of immunosuppressive medication on cellular and humoral immune responses in RMD patients receiving the inactivated COVID-19 vaccine.Methods:A total of 28 RMD patients and five healthy controls(HCs)receiving two doses of the inactivated COVID-19 vaccine(Sinovac-CoronaVac)were prospectively enrolled.Blood samples were collected before the primary vaccination(Week 0)and one week after the second vaccination(Week 5).Neutralizing antibody(nAb)titers and autoantibody titers were measured by a pseudovirus-based neutralization assay and enzyme-linked immunosorbent assay,respectively.CD4^(+)T-cell and CD19^(+)B-cell subsets and serum cytokines were analyzed by flow cytometry.Results:The inactivated COVID-19 vaccine was immunogenic in RMD patients and HCs after the second vaccination,but the nAb titers were lower in RMD patients than those in HCs.Only patients with systemic lupus erythematosus(SLE)had notably increased nAb titers.Remarkably,IgG^(+)CD27^(+),IgG^(+)IgG1^(+),and IgG^(+)IgG1^(−)B cells were reduced,whereas IgG−IgG1^(+)B cells,and total IgA and IgG titers were markedly increased.However,Tfh cell and Tfr cell subsets and cytokines produced by Tfh cells were not increased.The flare rate was low in RMD patients with comparable autoantibody titers,unchanged CD4^(+)T cell subsets and serum proinflammatory cytokines(interleukin[IL]-6,IL-17,interferon-γ,and tumor necrosis factor-α)after the second vaccination.Conclusions:Immunosuppressive therapy decreased the immunogenicity of the vaccine and maintained a low flare rate by selectively modulating B cell but not CD4^(+)T cell responses in RMD patients receiving the inactivated COVID-19 vaccine.Optimization of the treatment regimen might ensure a durable and robust COVID-19 vaccination response. | Rui Li Ruru Guo Jia Li Bing Zheng Ronghao Xu Xiaoyue Jiang Yingying Chen Guo Tang Qianqian Li Yixuan Li Jiaqi Feng Xiaoxiang Chen Ying Wang Shuang Ye Fang Du Liangjing Lu Jun Deng | 2023 | Rheumatology & Autoimmunity2023，3，2： | 0 |
| 17 | Construction of N,O co-doped carbon anchored with Co nanoparticles as efficient catalyst for furfural hydrodeoxygenation in ethanol显示文摘Hydrodeoxygenation of furfural(FF)into 2-methylfuran(MF)is a significant biomass utilization route.However,designing efficient and stable non-noble metal catalyst is still a huge challenge.Herein,we reported the N,O co-doped carbon anchored with Co nanoparticles(Co-SFB)synthesized by employing the organic ligands with the target heteroatoms.Raman,electron paramagnetic resonance(EPR),electrochemical impedance spectroscopy(EIS),and X-ray photoelectron spectroscopy(XPS)characterizations showed that the co-doping of N and O heteroatoms in the carbon support endows Co-SFB with enriched lone pair electrons,fast electron transfer ability,and strong metal-support interaction.These electronic properties resulted in strong FF adsorption as well as lower apparent reaction activation energy.At last,the obtained N,O co-doped Co/C catalyst showed excellent catalytic activity(nearly 100 mol%FF conversion and 94.6 mol%MF yield)and stability for in-situ dehydrogenation of FF into MF.This N,O co-doping strategy for the synthesis of highly efficient catalytic materials with controllable electronic state will provide an excellent opportunity to better understand the structure-function relationship. | Hui Yang Hao Chen Wenhua Zhou Haoan Fan Chao Chen Yixuan Sun Jiaji Zhang Sifan Wang Teng Guo Jie Fu | 2023 | Journal of Energy Chemistry2023，，3： | 0 |
| 18 | More Than Lightening:A Self-Supervised Low-Light Image Enhancement Method Capable for Multiple Degradations显示文摘Low-light images suffer from low quality due to poor lighting conditions,noise pollution,and improper settings of cameras.To enhance low-light images,most existing methods rely on normal-light images for guidance but the collection of suitable normal-light images is difficult.In contrast,a self-supervised method breaks free from the reliance on normal-light data,resulting in more convenience and better generalization.Existing self-supervised methods primarily focus on illumination adjustment and design pixel-based adjustment methods,resulting in remnants of other degradations,uneven brightness and artifacts.In response,this paper proposes a self-supervised enhancement method,termed as SLIE.It can handle multiple degradations including illumination attenuation,noise pollution,and color shift,all in a self-supervised manner.Illumination attenuation is estimated based on physical principles and local neighborhood information.The removal and correction of noise and color shift removal are solely realized with noisy images and images with color shifts.Finally,the comprehensive and fully self-supervised approach can achieve better adaptability and generalization.It is applicable to various low light conditions,and can reproduce the original color of scenes in natural light.Extensive experiments conducted on four public datasets demonstrate the superiority of SLIE to thirteen state-of-the-art methods.Our code is available at https://github.com/hanna-xu/SLIE. | Han Xu Jiayi Ma Yixuan Yuan Hao Zhang Xin Tian Xiaojie Guo | 2024 | IEEE/CAA Journal of Automatica Sinica2024，11，3： | 0 |
| 19 | Engineering polymer nanoparticles using cell membrane coating technology and their application in cancer treatments:Opportunities and challenges显示文摘Nanotechnology has revolutionized cancer drug delivery,and recent research continues to focus on the development of“one-size-fits-all,”i.e.,“all-in-one”delivery nanovehicles.Although nanomedicines can address significant shortcomings of conventional therapy,biological barriers remain a challenge in their delivery and accumulation at diseased sites.To achieve long circulation time,immune evasion,and targeted accumulation,conventional nanocarriers need modifications,e.g.,PEGylation,peptide/aptamer attachment,etc.One such modification is a biomimetic coating using cell membrane(CM),which can offer long circulation or targeting,or both.This top-down CM coating process is facile and can provide some advantageous features over surface modification by synthetic polymers.Herein,an overview is provided on the engineering of CM camouflaged polymer nanoparticles.A short section on CM and the development of CM coating technology has been provided.Detailed description of the preparation and characterization of CM camouflaged polymer NPs and their applications in cancer treatment has been reported.A brief comparison between CM coating and PEGylation has been highlighted.Various targeting approaches to achieve tumor-specific delivery of CM coated NPs have been summarized here.Overall,this review will give the readers a nice picture of CM coated polymer NPs,along with their opportunities and challenges. | Kai Guo Nanyang Xiao Yixuan Liu Zhenming Wang Judit Tóth János Gyenis Vijay Kumar Thakur Ayako Oyane Quazi T.H.Shubhra | 2022 | Nano Materials Science2022，4，4： | 0 |
| 20 | Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm显示文摘Self-organized blastoids from extended pluripotent stem(EPs)cells possess enormous potential for investigating postimplantation embryo development and related diseases.However,the limited ability of postimplantation development of Eps-blastoids hinders its further application.In this study,single-cell transcriptomic analysis indicated that the“trophectoderm(TE)-like structure”of EPSblastoids was primarily composed of primitive endoderm(PrE)-related cells instead of TE-related cells.We further identified PrE-like cells in EPS cell culture that contribute to the blastoid formation with TE-like structure.Inhibition of PrE cell differentiation by inhibiting MEK signaling or knockout of Gata6 in EPS cells markedly suppressed EPS-blastoid formation.Furthermore,we demonstrated that blastocyst-like structures reconstituted by combining the EPs-derived bilineage embryo-like structure(BLEs)with either tetraploid embryos or tetraploid TE cells could implant normally and develop into live fetuses.In summary,our study reveals that TE improvement is critical for constructing a functional embryo using stem cells in vitro. | Kuisheng Liu Xiaocui Xu Dandan Bai Yanhe Li Yalin Zhang Yanping Jia Mingyue Guo Xiaoxiao Han Yingdong Liu Yifan Sheng Xiaochen Kou Yanhong Zhao jiqing Yin Sheng Liu jiayu Chen Hong Wang Yixuan Wang Wenqiang Liu Shaorong Gao | 2023 | Protein & Cell2023，14，4： | 0 |
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