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| 1 | Characteristics of carbonate gas pool and multistage gas pool formation history of Hetianhe gas field, Tarim Basin, Northwest China显示文摘Hetianhe is a big carbonate gas field which isfound and demonstrated in the period of 'Chinese NationalNinth 5-Year Plan'. The proved reserve of Hetianhe gas fieldis over 600 ×10~8 m^3. Its main producing layers are Carbon-iferous bioclastic limestone and Ordovician carbonate com-posed of buried hill. The former is stratified gas pool withwater around its side, and the latter is massive gas pool withwater in its bottom. The gases in the gas pools belong to drygases with normal temperature and pressure systems. Basedon the correlation of gas and source rock, the gases aremainly generated from Cambrian source rocks. According tothe researches on source rock and structure evolution, andthe observations on the thin section to reservoir bitumen andthe studies on homogenization temperature of fluid inclu-sions, the gas pool has been identified and divided into threeformation periods. The first is Late Caledonian when the oilgenerated from the Cambrian source rocks and migratedalong faults, as a form of liquid facies into Ordovician carbonate res-ervoir and accumulated there. After that, the crustuplifted, the oil reservoir had been destroyed. The second isLate Hercynian when condensate gases generated from theCambrian source rocks and migrated into Ordovician res-ervoir, as a form of liquid facies. Since the fractures hadreached P strata, so the trap might have a real poor preser-vation condition, and the large-scale gas pool formation hadnot happened. The third gas reservoir formation period oc-curred in Himalaya. The fractures on both sides of Hetianhegas field developed violently under the forces of compression,and thus the present fault horst formed. The dry gases gen-erated from Cambrian source rocks and migrated upwardsas the form of gas facies into Ordovician and Carboniferousreservoirs, and the large gas pool as discovered at presentwas formed finally. | ZHOU Xinyuan JIA Chengzao WANG Zhaoming WANG Qinghua YANG Wei | 2002 | Chinese Science Bulletin2002，47，S1： | 13 |
| 2 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 3 | Generation of ready to use (RTU) products over China based on Landsat series data显示文摘Earth observation community has entered into the era of big data.Family of Landsat sensors have collected massive medium resolution satellite images,which are valuable for long-term land surface monitoring.In order to significantly reduce the magnitude of data processing for remote sensing data users,Landsat-based Ready to Use(RTU)products have been produced.Main RTU products,including orthorectified products,land surface reflectance,land surface temperature,large-area mosaic image,and standard image map products,are described.The resulting Landsat RTU products are hosted on the RSGS earth observation data sharing web site for free download(http://ids.ceode.ac.cn/rtu/).These new products will provide consistent,standardized,multi-decadal image data for robust land cover change detection and monitoring across the Earth sciences.In the coming years,CASEarth DataBank system will be constructed,which is an intelligent data service platform for providing not only the RTU products from multi-source satellite data,but also big earth data analysis methods. | Guojin He Zhaoming Zhang Weili Jiao Tengfei Long Yan Peng Guizhou Wang Ranyu Yin Wei Wang Xiaomei Zhang Huichan Liu Bo Cheng Bo Xiang | 2018 | Big Earth Data2018，2，1： | 8 |
| 4 | The nitritation performance of biofilm reactor for treating domestic wastewater under high dissolved oxygen显示文摘The objective of this study was to investigate the nitritation performance in a biofilm reactor for treating domestic wastewater.The reactor was operated in continuous feed mode from phases 1 to 3.The dissolved oxygen(DO)was controlled at 3.5–7 mg/L throughout the experiment.The biofilm reactor showed excellent nitritation performance after the inoculation of nitrifying sludge,with the hydraulic retention time being reduced from 24 to 7 hr.Above 90%nitrite accumulation ratio(NAR)was maintained in phase 1.Afterwards,nitratation occurred with the low NH_4^+–N concentration in the reactor.The improvement of NH_4^+–N concentration to 20–35 mg/L had a limited effect on the recovery of nitritation.However,nitritation recovered rapidly when sequencing batch feed mode was adopted in phase 4,with the effluent NH_4^+-N concentration above 7 mg/L.The improvement of ammonia oxidizing bacteria(AOB)activity and the combined inhibition effect of free ammonia(FA)and free nitrous acid(FNA)on the nitrite oxidizing bacteria(NOB)were two key factors for the rapid recovery of nitritation.Sludge activity was obtained in batch tests.The results of batch tests had a good relationship with the long term operation performance of the biofilm reactor. | Zhaoming Zheng Zebing Li Jing Ma Jia Du Guanghui Chen Wei Bian Jun Li Baihang Zhao | 2016 | Journal of Environmental Sciences2016，28，4： | 7 |
| 5 | Inhibition factors and Kinetic model for ammonium inhibition on the anammox process of the SNAD biofilm显示文摘The aim of the present work was to evaluate the anaerobic ammonium oxidation(anammox) activity of simultaneous partial nitrification, anammox and denitrification(SNAD) biofilm with different substrate concentrations and p H values. Kaldnes rings taken from the SNAD biofilm reactor were incubated in batch tests to determine the anammox activity. Haldane model was applied to investigate the ammonium inhibition on anammox process. As for nitrite inhibition, the NH_(4^-)^+N removal rate of anammox process remained 87.4% of the maximum rate with the NO_(2^-)^-N concentration of 100 mg/L. Based on the results of Haldane model, no obvious difference in kinetic coefficients was observed under high or low free ammonia(FA) conditions, indicating that ammonium rather than FA was the true inhibitor for anammox process of SNAD biofilm. With the p H value of 7.0, the r max, Ks and K_I of ammonium were 0.209 kg NO_(2^-)^-N/kg VSS/day, 9.5 mg/L and 422 mg/L, respectively. The suitable p H ranges for anammox process were 5.0 to 9.0. These results indicate that the SNAD biofilm performs excellent tolerance to adverse conditions. | Zhaoming Zheng Jun Li Jing Ma Jia Du Fan Wang Wei Bian Yanzhuo Zhang Baihang Zhao | 2017 | Journal of Environmental Sciences2017，29，3： | 5 |
| 6 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 7 | A joint call for actions to advance taxonomy in China显示文摘Taxonomy plays an important role in understanding the origin, evolution, and ecological functionality of biodiversity. There are large number of unknown species yet to be described by taxonomists, which together with their ecosystem services cannot be effectively protected prior to description. Despite this, taxonomy has been increasingly underrated insufficient funds and permanent positions to retain young talents. Further, the impact factordriven evaluation systems in China exacerbate this downward trend, so alternative evaluation metrics are urgently necessary. When the current generation of outstanding taxonomists retires,there will be too few remaining taxonomists left to train the next generation. In light of these challenges, all co-authors worked together on this paper to analyze the current situation of taxonomy and put out a joint call for immediate actions to advance taxonomy in China. | Chaodong Zhu Arong Luo Ming Bai Michael COrr Zhonge Hou Siqin Ge Jun Chen Yibo Hu Xuming Zhou Gexia Qiao Hongzhi Kong Limin Lu Xiaohua Jin Lei Cai Xinli Wei Ruilin Zhao Wei Miao Qingfeng Wang Zhongli Sha Qiang Lin Meng Qu Jianping Jiang Jiatang Li Jing Che Xuelong Jiang Xiaoyong Chen Lianming Gao Zongxin Ren Chunlei Xiang Shixiao Luo Donghui Wu Dong Liu Yanqiong Peng Tao Su Chenyang Cai Tianqi Zhu Wanzhi Cai Xingyue Liu Hu Li Huaijun Xue Zhen Ye Xuexin Chen Pu Tang Shujun Wei Hong Pang Qiang Xie Feng Zhang Feng Zhang Xianjin Peng Aibing Zhang Taiping Gao Changfa Zhou Chen Shao Libin Ma Zhaoming Wei Yunxia Luan Ziwei Yin Wu Dai Cong Wei Xiaolei Huang Jingxian Liu Xiangsheng Chen Tianci Yi Zhisheng Zhang Zhulidezi Aishan Qin Li Hongying Hu | 2022 | Zoological Systematics2022，47，3： | 1 |
| 8 | Structures of signaling complexes of lipid receptors S1PR1 and S1PR5 reveal mechanisms of activation and drug recognition显示文摘Sphingosine-1-phosphate(S1P)is an important bioactive lipid molecule in cell membrane metabolism and binds to G proteincoupled S1P receptors(S1PRs)to regulate embryonic development,physiological homeostasis,and pathogenic processes in various organs.S1PRs are lipid-sensing receptors and are therapeutic targets for drug development,including potential treatment of COVID-19.Herein,we present five cryo-electron microscopy structures of S1PRs bound to diverse drug agonists and the heterotrimeric Gi protein.Our structural and functional assays demonstrate the different binding modes of chemically distinct agonists of S1PRs,reveal the mechanical switch that activates these receptors,and provide a framework for understanding ligand selectivity and G protein coupling. | Yuan Yuan Guowen Jia Chao Wu Wei Wang Lin Cheng Qian Li Ziyan Li Kaidong Luo Shengyong Yang Wei Yan Zhaoming Su Zhenhua Shao | 2021 | Cell Research2021，31，12： | 1 |
| 9 | Joint hybrid beamforming and power splitting design in millimeter-wave simultaneous wireless information and power transfer system显示文摘A joint hybrid beamforming and power splitting(JHBPS) design problem for simultaneous wireless information and power transfer(SWIPT) in millimeter-wave(mmWave) system is studied.The considered scenario is a multi-antenna base station(BS) transfers information and energy simultaneously to multiple single-antenna receivers.BS adopts hybrid digital and analog beamforming architecture to reduce hardware costs.Receivers separate acquired signals with power splitters either for information decoding(ID) or energy harvesting(EH).The aim is minimizing total transmission power by joint design of hybrid beamforming and PS under ID and EH requirements.It is difficult to obtain the optimal hybrid beamformer directly since the analog beamformer and digital beamformer are multiplied.Therefore,a two-stage algorithm is proposed to solve the problem.In the first stage,the optimal beamformer and PS ratios are obtained by solving the joint transmission beamforming and PS design problem.In the second stage,the optimal beamformer is approximated with the product of analog beamformer and digital beamformer.The superiority of proposed algorithm over the existing algorithms is demonstrated through simulations.Moreover,the effectiveness of approximation algorithm is testified. | Bao Tiantian Wen Xiangming Lu Zhaoming Chen Yawen Zheng Wei | 2019 | The Journal of China Universities of Posts and Telecommunications2019，26，2： | 1 |
| 10 | Generation of gene-target dogs using CRISPR/Cas9 system显示文摘Dear Editor,Dogs(Canis familiaris)serve as human companions and are raised to herd livestock,aid hunters,guard homes,perform police and rescue work,and guide the blind.Dogs exhibit close similarities to humans in terms of metabolic,physiological,and anatomical characteristics,and thus are ideal genetic and clinical models to study human diseases(Tsai et al.,2007).Gene target technology is a powerful tool to create new strains of animals with favorable traits.However,thus far,gene-target dogs have not been developed due to their unique species-specific reproductive characteristics,which limits the applications of dogs especially in the field of biomedical research.Recently,clustered regularly interspaced short palindromic repeats(CRISPRs)/CRISPR-associated(Cas)9 system was applied to edit specific genes with a high efficiency(Cong et al.,2013;Mali et al.,2013).Here we attempt to explore the feasibility of producing gene knockout(KO)dogs by using this technology.Beagle dog,the most widely used breed in biomedical research,was used as our animal model.Myostatin(MSTN)was chosen as the first gene of interest. | Qingjian Zou Xiaomin Wang Yunzhong Liu Zhen Ouyang Haibin Long Shu Wei Jige Xin Bentian Zhao Sisi Lai Jun Shen Qingchun Ni Huaqiang Yang Huilin Zhong Li Li Minhua Hu Quanjun Zhang Zhidong Zhou Jiaxin He Quanmei Yan Nana Fan Yu Zhao Zhaoming Liu Lin Guo Jiao Huang Guanguan Zhang Jun Ying Liangxue Lai Xiang Gao | 2015 | Journal of Molecular Cell Biology2015，7，6： | 1 |
| 11 | Hawanoids A–E, unprecedented diterpenoids with PAF-induced platelet aggregation inhibitory activities from the deep-sea-derived fungus Paraconiothyrium hawaiiense显示文摘Hawanoids A–E(1–5), five highly cyclized diterpenoids were isolated from the deep-sea-derived fungus Paraconiothyrium hawaiiense FS482. Compounds 1 and 2 possessed an unprecedented tetracyclo[6.6.2.0^(2,7).0^(11,15)]cetane carbon skeleton while 3 and 4 possessed an unusual 11,14-macrocyclic ether moiety in phomactin family. Their structures including the stereo-chemistry were determined through spectroscopic analysis, X-ray diffractions and computational calculations. The plausible biosynthetic pathway was proposed based on the predicted biosynthetic gene cluster. All of the isolated compounds exhibited inhibitory activities against PAF-induced platelet aggregation. The molecular docking study was carried out understand the interaction between the PAF receptor and hawanoids with different skeletons. | Shushuai Chen Hongxin Liu Saini Li Yuchan Chen Wei Ye Haohua Li Haibo Tan Dongli Li Zhaoming Liu Weimin Zhang | 2023 | Chinese Chemical Letters2023，34，2： | 0 |
| 12 | Molecular phylogeny and divergence time estimates using the mitochondrial genome for the hadal snailfish from the Mariana trench显示文摘Snailfishes are an important component of the hadal fish fauna[1].Based on osteological and other morphological characters,Kido[2]recognized the Cyclopteridae as a sister group to the Liparidae and the superfamily Cottoidea as a sister group to the two families.The close relationship between Liparidae and Cyclopteridae is | Yanjun Shen Wei Dai Zhaoming Gao Guoyong Yan Xiaoni Gan Shunping He | 2017 | Science Bulletin2017，62，16： | 0 |
| 13 | Generation of pigs with humanized type Ⅱ collagen by precise human COL2A1 gene knock-in显示文摘Osteoarthritis(OA)is the most common chronic disease,characterized by progressive cartilage breakdown,subchondral bone sclerosis,and aberrant bone outgrowth(Yucesoy et al.,2015;Hussain et al.,2016).OA is one of the leading causes of cartilage damage.Patients with severe cartilage damage require transplantation of articular cartilage to improve their quality of life.Type Ⅱ collagen is a major component of articular cartilage and intervertebral discs and plays an important role in the structure and strength of connective tissues that support muscles and joints(Byers,1994). | Ting Lan Yuling Zheng Yangyang Suo Yuhui Wei Hui Shi Quanmei Yan Zhenpeng Zhuang Huangyao Chen Quanjun Zhang Nana Fan Yu Zhao Zhen Ouyang Chengdan Lai Zhaoming Liu Jizeng Zhou Chengcheng Tang Nam-Hyung Kim Qingjian Zou Xiaomin Wang | 2023 | Journal of Genetics and Genomics2023，50，3： | 0 |
| 14 | The role of CDK1 siRNA interference in cell cycle and cell apoptosis显示文摘In the present report,cyclin-dependent kinase1(CDK1)siRNA was transfected into cells to silence the CDK1 gene expression and study its role in the cell cycle and cell apoptosis.The siRNA targeting CDK1 gene was chemically synthesized and transfected into Hela cells by lipofectamine 2000.The expression levels of CDK1 gene and protein were examined by real-time quantitative polymerase chain reaction(PCR)and Western blot,respectively.The cell cycle was analyzed by using DNA content analysis byflow cytometry.Cell apoptosis was detected by the Annexin V/PI method.The morphological changes of transfected cells were examined under the microscopy by Wright-Giemsa stain.CDK1 gene was successfully silenced by its siRNA,and the CDK1 protein expression level was decreased significantly,especially from 48th h to 60th h after transfection.The DNA content analysis showed that transfection of CDK1 siRNA led to cells accumulating in G2/M phase.There was no significant difference in the apoptotic rate between transfected cells and the control cells after transfection of CDK1 siRNA for 48 or 60 h.More double nucleus or multinucleus cells could be seen under the microscopy among the transfected cells.The decreased CDK1 expression by siRNA silencing gave rise to cell cycle arrest in G2/M phase but did not induce apoptosis. | Hui XIAO Ming TIAN Junna GE Xin WEI Zhaoming LI Xiaolan LI Deding TAO Junbo HU Jianping GONG | 2009 | Frontiers of Medicine2009，3，4： | 0 |
| 15 | Structural basis of sRNA RsmZ regulation of Pseudomonas aeruginosa virulence显示文摘Dear Editor,Pseudomonas aeruginosa is a ubiquitous Gram-negative opportunistic bacterium that notoriously causes infections with a high mortality rate in hospitalized patients,especially those with compromised immune systems.P.aeruginosa can cause acute infections that are typically associated with the cytotoxins secreted by the type Ⅲ secretion system(T3ss). | Xinyu Jia Zhiling Pan Yang Yuan Bingnan Luo Yongbo Luo Sunandan Mukherjee Guowen Jia Liu Liu Xiaobin Ling Xiting Yang Zhichao Miao Xiawei Wei Janusz M.Bujnicki Kelei Zhao Zhaoming Su | 2023 | Cell Research2023，33，4： | 0 |
| 16 | Weldable and closed-loop recyclable monolithic dynamic covalent polymer aerogels显示文摘Owing to their low density,high porosity and unique micro-nanostructures,aerogels are attractive for application in various fields;however,they suffer from shrinkage and/or cracking during preparation,mechanical brittleness,low production efficiency and non-degradation.Herein,we introduce the concept of dynamic covalent polymer chemistry to produce a new class of aerogels—referred to as DCPAs.The resulting lightweight DCPAs have the potential to be prepared on a large scale and feature high porosity(90.7%–91.3%),large degrees of compression(80%strain)and bending(diametral deflection of 30 mm)without any cracks,as well as considerable tensile properties(an elongation with a break at 32.7%).In addition,the DCPAs showcase the emergent characteristics of weldability,repairability,degradability and closed-loop recyclability that are highly desirable for providing versatile material platforms,though hardly achieved by traditional aerogels.Taking advantage of their robust porous structures,we demonstrate the potential of DCPAs for applications in thermal insulation and emulsion separation.These findings reveal that the dynamic covalent bond strategy would be generalized for the production of a new generation of aerogels with customized features for functioning in the field of intelligent and sustainable materials. | Xinhai Zhang Jun Zhao Kai Liu Guangfeng Li Dong Zhao Zhaoming Zhang Junjun Wan Xue Yang Ruixue Bai Yongming Wang Wei Zhang Xuzhou Yan | 2022 | National Science Review2022，9，9： | 0 |
| 17 | Genomic landscape of T-cell lymphoblastic lymphoma显示文摘Objective: T-cell lymphoblastic lymphoma(T-LBL) is an aggressive neoplasm of precursor T cells, however,detailed genome-wide sequencing of large T-LBL cohorts has not been performed due to its rarity. The purpose of this study was to identify putative driver genes in T-LBL.Methods: To gain insight into the genetic mechanisms of T-LBL development, we performed whole-exome sequencing on 41 paired tumor-normal DNA samples from patients with T-LBL.Results: We identified 32 putative driver genes using whole-exome sequencing in 41 T-LBL cases, many of which have not previously been described in T-LBL, such as Janus kinase 3(JAK3), Janus kinase 1(JAK1), Runtrelated transcription factor 1(RUNX1) and Wilms’ tumor suppressor gene 1(WT1). When comparing the genetic alterations of T-LBL to T-cell acute lymphoblastic leukemia(T-ALL), we found that JAK-STAT and RAS pathway mutations were predominantly observed in T-LBL(58.5% and 34.1%, respectively), whereas Notch and cell cycle signaling pathways mutations were more prevalent in T-ALL. Notably, besides notch receptor 1(NOTCH1), mutational status of plant homeodomain(PHD)-like finger protein 6(PHF6) was identified as another independent factor for good prognosis. Of utmost interest is that co-existence of PHF6 and NOTCH1 mutation status might provide an alternative for early therapeutic stratification in T-LBL.Conclusions: Together, our findings will not only provide new insights into the molecular and genetic mechanisms of T-LBL, but also have tangible implications for clinical practice. | Zhaoming Li Yue Song Mingzhi Zhang Yiming Wei Hang Ruan | 2022 | Chinese Journal of Cancer Research2022，34，2： | 0 |
| 18 | 敲入亨廷顿基因的猪模型再现了亨廷顿病的选择性神经退化症状显示文摘文章简介亨廷顿舞蹈病(Huntington’s disease, HD)是一种遗传性神经退行性疾病,是由一种编码导致脑细胞死亡的毒性蛋白的基因引起的。尽管转基因小鼠已被广泛用于构建神经退行性疾病模型,但是它们缺乏在人类大脑中观察到的典型神经变性或明显的神经元缺失。 | Sen Yan Zhuchi Tu Zhaoming Liu Nana Fan Huiming Yang Su Yang Weili Yang Yu Zhao Zhen Ouyang Chengdan Lai Huaqiang Yang Li Li Qishuai Liu Hui Shi Guangqing Xu Heng Zhao Hongjiang Wei Zhong Pei 李世华 赖良学 李晓江 | 2019 | 科学新闻2019，0，2： | 0 |
| 19 | Massage and vocal training in a case of spasmodic dysphonia显示文摘A 35-year-old female patient with spasmodic dysphonia underwent massage and vocal training.Chinese manipulation was administered to Tiantu(CV 22) and Lianquan(CV 23),the convergent acupoints of the conception meridian and Yin link vessel,Yamen(GV 15) and Fengfu(GV 16),the convergent acupoints of Du meridian and Yang link vessel,as well as large intestine channel of hand Yangming Hegu(LI 4),by gently pressing and rubbing for stimulation.In addition,throat muscle massage therapy and vocal training were performed.Results were evaluated using acoustic measurements in combination with a single statistical method.A comprehensive treatment combining vocal training and face-neck massage was shown to relieve dystonia of facial-cervical muscles in patients,as well as improve voice quality while providing a safe treatment method. | Ping Wan Kexing Sun Zhaoming Huang Xin Wei | 2010 | Neural Regeneration Research2010，5，23： | 0 |
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