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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | The altitude effect of ^(18)δO in precipitation and river water in the Southern Himalayas显示文摘The lapse rate of water isotopes is used in the study of the hydrologic cycle as well as in the estimation of uplift of the Tibetan Plateau.The greater elevation contrast in the Southern Himalayas allows for a detailed discussion about this lapse rate.We analyze variations of 18δO in precipitation and river water between 1320 m and 6700 m elevations in the Southern Himalayas,and calculate the specific lapse rate of water 18δO.The results show that the multi-year average lapse rate in precipitation over this region is 0.15‰/100 m.The one-year average lapse rate is 0.17‰/100 m from three sites along the Southern Himalayas.The two results agree,but are much lower than the global average of 0.28‰/100 m.This work also shows that there is a difference in precipitation 18δO lapse rate between the monsoon and non-monsoon seasons.The calculated precipitation lapse rate is much lower than that in surface water. | WEN Rong TIAN LiDe WENG YongBiao LIU ZhongFang ZHAO ZhongPing | 2012 | Chinese Science Bulletin2012，57，14： | 11 |
| 3 | Seasonal variations of stable isotope in precipitation and moisture transport at Yushu,eastern Tibetan Plateau显示文摘Precipitation δ 18O at Yushu, eastern Tibetan Plateau, shows strong fluctuation and lack of clear seasonality. The seasonal pattern of precipitation stable isotope at Yushu is apparently different from either that of the southwest monsoon region to the south or that of the inland region to the north. This different seasonal pattern probably reflects the shift of different moisture sources. In this paper, we present the spatial comparison of the seasonal patterns of precipitation δ 18O, and calculate the moisture transport flux by using the NCAR/NCEP reanalysis data. This allows us to discuss the relation between moisture transport flux and precipitation δ 18O. This study shows that both the southwest monsoon from south and inland air mass transport from north affected the seasonal precipitation δ 18O at Yushu, eastern Tibetan Plateau. Southwest monsoon brings the main part of the moisture, but southwest transport flux is weaker than in the southern part of the Tibetan Plateau. However, contribution of the inland moisture from north or local evaporation moisture is enhanced. The combined effect is the strong fluctuation of summer precipitation δ 18O at Yushu and comparatively poor seasonality. | TIAN LiDe MA LingLong YU WuSheng LIU ZhongFang YIN ChangLiang ZHAO ZhongPing TANG Wei WANG Yu | 2008 | Science China Earth Sciences2008，51，8： | 9 |
| 4 | μ-and m-calpain expression and activity changes following diethylstilbestrol injection in the rat anterior pituitary显示文摘Little is known about changes in calpain activity in the pituitary gland.In the present study,μ-and m-calpain activity changes were detected in the rat anterior pituitary following intraperitoneal injection of diethylstilbestrol.Double-immunofluorescence labeling confirmed colocalization of μ-and m-calpain in prolactin-secreting cells(lactotrophs).Western blot analysis revealed significantly increased expression of both calpains,which accompanied upregulated cytosol and membrane zymographic activities at 12 weeks following diethylstilbestrol injection,compared with rats injected with sunflower oil.Moreover,following estrogen injection,pituitary gland pathological damage gradually worsened with increasing time.Results demonstrated that estrogen regulated calpain expression and activity,and both calpains participated in the pathophysiological processes of the pituitary gland.Ubiquitous calpain expression could serve as an effective target for anti-estrogen drugs. | Weijiang Zhao Zhongfang Shi Fang Yuan Guilin Li Yazhuo Zhang Zhongcheng Wang | 2011 | Neural Regeneration Research2011，6，1： | 7 |
| 5 | Safety and superior immunogenicity of heterologous boosting with an RBD-based SARS-CoV-2 mRNA vaccine in Chinese adults显示文摘Dear Editor,To May 2022,the COVID-19 pandemic has claimed more than 6.28 million lives,with more than 524 million confirmed cases worldwide.The recent emergence of highly transmissible Omicron variant of severe acute respiratory syndrome coronavirus 2(SARSCoV-2)has triggered another major surge in both confirmed cases and deaths.1 Ten COVID-19 vaccines have been approved by the World Health Organization(WHO)for emergency use,including the two mRNA vaccines,BNT162b2 and mRNA-1273,and two Chinese inactivated vaccines,CoronaVac and BBIBP-CorV.However,the rapid waning of vaccine-induced virus-neutralizing antibody titers and the continuous emergence of variants of concern(VOCs),including Alpha,Beta,Delta and Omicron,have created unprecedented challenges in the eradication of COVID-19 pandemic.2–4. | Xiaoqiang Liu Yuhua Li Zhongfang Wang Shouchun Cao Weijin Huang Lin Yuan Yi-Jiao Huang Yan Zheng Jingjing Chen Bo Ying Zuoyun Xiang Jin Shi Jincun Zhao Zhen Huang Cheng-Feng Qin | 2022 | Cell Research2022，32，8： | 5 |
| 6 | Adsorption of cesium from aqueous solution using agricultural residue – Walnut shell: Equilibrium, kinetic and thermodynamic modeling studies显示文摘 | Dahu Ding Yingxin Zhao Shengjiong Yang Wansheng Shi Zhenya Zhang Zhongfang Lei Yingnan Yang | 2013 | Water Research2013，，： | 4 |
| 7 | N-heterocyclic carbene as a promising metal-free electrocatalyst with high efficiency for nitrogen reduction to ammonia显示文摘Electrocatalytic nitrogen reduction reaction(NRR)at ambient conditions holds great promise for sustainably synthesizing ammonia(NH3),while developing highly-efficient,long-term stable,and inexpensive catalysts to activate the inert N≡N bond is a key scientific issue.In this work,on the basis of the concept'N-heterocyclic carbenes(NHCs)',we propose a carbon decorated graphitic-carbon nitride(C/g-C3N4)as novel metal-free NRR electrocatalyst by means of density functional theory(DFT)computations.Our results reveal that the introduced C atom in g-C3N4 surface can be regarded as NHCs and catalytic sites for activating N≡N bond,and are stabilized by the g-C3N4 substrate due to sterically disfavored dimerization.Especially,this NHCs-based heterogeneous catalysis can efficiently reduce the activated N2 molecule to NH3 with a low overpotential of 0.05 V via an enzymatic mechanism.Our work is the first report of NHCs-based electrocatalyst for N2 fixation,thus opening an alternative avenue for advancing sustainable NH3 production. | Hongyan Li Le Yang Zhongxu Wang Peng Jin Jingxiang Zhao Zhongfang Chen | 2020 | Journal of Energy Chemistry2020，29，7： | 3 |
| 8 | Melatonin modulates the effects of diethylstilbestrol (DES) on the anterior pituitary of the female Wistar rat显示文摘 | Zhao Weijiang Shi Zhongfang Yuan Fang Li Guilin Sun Yilin Zhang Yazhuo Wang Zhongcheng | 2010 | Folia Histochemica et Cytobiologica2010，，2： | 1 |
| 9 | Long-range gene regulation network of the MGMT enhancer modulates glioma cell sensitivity to temozolomide显示文摘Approximately 80%of primary tumors in the central nervous system are malignant glioma,and>50%of those are diagnosed as glioblastoma(GBM),the most aggressive glioma.Despite treatment advances,GBM prognosis is unsatisfying,and the median survival time of patients is only 14-17 months. | Bohan Chen Anshun He Jinfang Bi Shupeng Sun Yiping Ma Wenbin Wang Dianhao Guo Jun Chen Yuyang Qian Tengfei Shi Guohui Nie Zhongfang Zhao Jiandang Shi Hongzhen Yang Lei Zhang Wange Lu | 2021 | Journal of Genetics and Genomics2021，48，10： | 1 |
| 10 | Effective adsorption of Cr (VI) from aqueous solution using natural Akadama clay显示文摘 | Yingxin Zhao Shengjiong Yang Dahu Ding Jie Chen Yingnan Yang Zhongfang Lei Chuanping Feng Zhenya Zhang | 2013 | Journal of Colloid And Interface Science2013，，： | 1 |
| 11 | Unveiling the mechanisms of Fe(Ⅲ)-loaded chitosan composite(CTS-Fe)in enhancing anaerobic digestion of waste activated sludge显示文摘Anaerobic digestion(AD)of waste activated sludge(WAS)is usually limited by the low generation efficiency of methane.Fe(Ⅲ)-loaded chitosan composite(CTS-Fe)have been reported to effectively enhanced the digestion of WAS,but its role in promoting anaerobic sludge digestion remains unclear.In present study,the effects of CTS-Fe on the hydrolysis and methanogenesis stages of WAS anaerobic digestion were investigated.The addition of CTSFe increased methane production potential by 8%-23%under the tested conditions with the addition of 5-20 g/L CTS-Fe.Besides,the results demonstrate that the addition of CTS-Fe could effectively promote the hydrolysis of WAS,evidenced by lower protein or polysaccharides concentration,higher soluble organic carbon in rector adding CTS-Fe,as well as the increased activity of extracellular hydrolase with higher CTS-Fe concentration.Meanwhile,the enrichment of Clostridia abundance(iron-reducing bacteria(IRBs))was observed in CTS-Fe adding reactor(8.9%-13.8%),which was higher than that in the control reactor(7.9%).The observation further suggesting the acceleration of hydrolysis through dissimilatory iron reduction(DIR)process,thus providing abundant substrates for methanogenesis.However,the presence of CTS-Fe was inhibited the acetoclastic and hydrogenotrophic methanogenesis process,which could be ascribed to the Fe(Ⅲ)act as electron acceptor coupled to methane for anaerobic oxidation.Furthermore,coenzyme F420 activity in the CTS-Fe added reactor was 34.9% lower than in the blank,also abundance of microorganisms involved in hydrogenotrophic methanogenesis was decreased.Results from this study could provide theoretical support for the practical applications of CTS-Fe. | Boaiqi Zhang Ziwen Zhao RuiMa Nan Chen Zhe Kong Zhongfang Lei Zhenya Zhang | 2024 | Journal of Environmental Sciences2024，，4： | 0 |
| 12 | Long-term application of diethylstilbestrol upregulates expressions of μ-and m-calpains in pituitary intermediate lobe of female Wistar rats显示文摘BACKGROUND: During formation of prolactin neoplasia, how cells and its structure in adenohypophysis affect prolactin cells should be further studied. Intermediate lobe can be regarded as a driving region to release prolactin (PRL) and may promote formation of prolactin neoplasia in pituitary anterior lobe. OBJECTIVE: To observe the effect of diethylstilbestrol (DES) on the expressions of μ and m-calpains in pituitary intermediate lobe of female Wistar rats. DESIGN: Observational contrast animal study. SETTING: Beijing Neurosurgical Institute. MATERIALS: A total of 21 female Wistar rats, 3 weeks old weighing 70–80 g were housed with free access to tap water and standard pellet food. They were kept in a CL-grade condition, at (24±1)℃ and a humidity of (55±5)%, and with a 12 hours day-night cycle. Caprine anti-μ- and m-calpains antibodies were provided by Santa Cruz Biotechnology, CA, USA; rabbit-anti-PRL antibodies by Dako, Denmark; rabbit-anti-ACTH antibody by Boster Company, Wuhan. METHODS: The experiment was carried out in Pathophysiological Department and Animal Laboratory, Beijing Neurosurgical Institute from August 2006 to January 2007. ① Rats were randomly divided into groups with 7 in each group, including vehicle control group, in which rats were injected intraperitoneally with sun-flower seed oil (1 mL/kg, twice a week) for 16 weeks; DES group, where animals were administered with DES (5 mg/kg, twice a week) for 16 weeks; DES + vehicle control group, in which DES was administered for 12 weeks at the same dose with those in DES group, and then was discontinued and replaced by sun-flower seed oil (1 mL/kg, twice a week) for the following 4 weeks. ② At 16 weeks later, pituitary tissue was dealt with HE staining and PRL immunohistochemical examination to observe evoke of tumor; meanwhile, immunohistochemical examination was used to observe expression of PRL of pituitary anterior lobe, expressions of μ- and m-calpains of pituitary intermediate lobe and distribution of adrenocorticotropin. MAIN OUTCOME MEASURES: ① Expression of PRL of pituitary anterior lobe, expressions of μ- and m-calpains of pituitary intermediate lobe and distribution of adrenocorticotropin. ② Morphological observation of pituitary tissue. RESULTS: All 21 rats were involved in the final analysis. ① Results of immunohistochemical examination: Morphological changes of neoplasia in DES group were strongly positive to PRL, and this suggested that formation of prolactin adenoma was observed in pituitary tissue. As compared with vehicle control group, expression of adrenoeorticotropic hormone (ACTH) was increased in both DES group and DES + vehicle control group. In addition, expressions of μ- and m-calpains in pituitary intermediate lobe were higher in DES group than that in vehicle control group. Otherwise, expressions of m-calpains in pituitary intermediate lobe was decreased in DES + vehicle control group, but expression of μ-calpains was still increased. ② Morphological observation of pituitary tissue: Gland tubes were orderly arranged in rats in vehicle control group. Anterior pituitary gland in rats of DES group demonstrated an apparent disappearance of gland tubes and a relatively large-scaled vasculature formation, namely the vascular lake lined by tightly arranged endothelial cells. Local integrated tumor cell arrangements were also detected. In addition, the border between the IL and the anterior lobe was locally blurred. The definite tumor-like changes in pituitary tissueswere confirmed in 6 of 7 female Wistar rats in DES group, and one spontaneous occurrence of tumor formation was found in vehicle control group. In DES + vehicle control group, DES withdrawal led to the subtile emergence of gland tube cavity, although tumor-like cells still existed in 4 of 7 rats, suggesting occurrence of the tumor regression due to the withdrawal of DES. CONCLUSION: A long-term application of DES can enhance the expressions of ubiquitours neutral cysteine protease in pituitary intermediate lobe and this suggests that both of them play a key role in release of hormone and formation of prolactin neoplasia through directly promoting PRL expression and release of neighboring pituitary intermediate lobe. | Weijiang Zhao Fang Yuan Guilin Li Zhongfang Shi Yun Cui Yazhuo Zhang Zhongcheng Wang | 2007 | Neural Regeneration Research2007，2，5： | 0 |
| 13 | Breaking the responsivity-speed dilemma of a-GaO_(x) photodetector by alternating gate modulation显示文摘The gallium oxide(Ga_(2)O_(3))based photodetectors are usually confronted with the typical challenge of the responsivity-speed(RS)dilemma,namely high photocurrent induces a long decay time,as shown in Figure 1(a).The RS dilemma involves a trade-off issue,which is independent of photoelectric materials. | Zhongfang ZHANG Pengju TAN Xiaohu HOU Xiaolan MA Mengfan DING Shunjie YU Guangwei XU Xiaolong ZHAO Shibing LONG | 2023 | Science China(Information Sciences)2023，66，12： | 0 |
| 14 | Single-atom catalysts with anionic metal centers: Promising electrocatalysts for the oxygen reduction reaction and beyond显示文摘Ongoing efforts to develop single-atom catalysts(SACs) for the oxygen reduction reaction(ORR) typically focus on SACs with cationic metal centers,while SACs with anionic metal centers(anionic SACs) have been generally neglected.However,anionic SACs may offer excellent active sites for ORR,since anionic metal centers could facilitate the activation of O_(2) by back donating electrons to the antibonding orbitals of O_(2).In this work,we propose a simple guideline for designing anionic SACs:the metal centers should have larger electronegativity than the surrounding atoms in the substrate on which the metal atoms are supported.By means of density functional theory(DFT) simulations,we identified 13 anionic metal centers(Co,Ni,Cu,Ru,Rh,Pd,Ag,Re,Os,Ir,Pt,Au,and Hg) dispersed on pristine or defective antimonene substrates as new anionic SACs,among which anionic Au and Co metal centers exhibit limiting potentials comparable to,or even better than,conventional Pt-based catalysts towards ORR.We also found that anionic Os and Re metal centers on the defective antimonene can electrochemically catalyze the nitrogen reduction reaction(NRR) with a limiting potential close to that of stepped Ru(0001).Overall,our work shows promise towards the rational design of anionic SACs and their utility for applications as electrocatalysts for ORR and other important electrochemical reactions. | Jinxing Gu Yinghe Zhao Shiru Lin Jingsong Huang Carlos R.Cabrera Bobby G.Sumpter Zhongfang Chen | 2021 | Journal of Energy Chemistry2021，30，12： | 0 |
| 15 | Innate immune imprints in SARS-CoV-2 Omicron variant infection convalescents显示文摘SARS-CoV-2 Omicron variant infection generally gives rise to asymptomatic to moderate COVID-19 in vaccinated people.The immune cells can be reprogrammed or“imprinted”by vaccination and infections to generate protective immunity against subsequent challenges.Considering the immune imprint in Omicron infection is unclear,here we delineate the innate immune landscape of human Omicron infection via single-cell RNA sequencing,surface proteome profiling,and plasma cytokine quantification.We found that monocyte responses predominated in immune imprints of Omicron convalescents,with IL-1β-associated and interferon(IFN)-responsive signatures with mild and moderate symptoms,respectively.Low-density neutrophils increased and exhibited IL-1β-associated and IFN-responsive signatures similarly.Mild convalescents had increased blood IL-1β,CCL4,IL-9 levels and PI3+neutrophils,indicating a bias to IL-1βresponsiveness,while moderate convalescents had increased blood CXCL10 and IFN-responsive monocytes,suggesting durative IFN responses.Therefore,IL-1β-or IFN-responsiveness of myeloid cells may indicate the disease severity of Omicron infection and mediate post-COVID conditions. | Zhiqing Li Xiaosu Chen Junyan Dan Tianju Hu Ye Hu Shuxun Liu Yangyang Chai Yansong Shi Jian Wu Hailai Ni Jiaqi Zhu Yanfeng Wu Nan Li Yizhi Yu Zhongfang Wang Jincun Zhao Nanshan Zhong Xianwen Ren Zhongyang Shen Xuetao Cao | 2022 | Signal Transduction and Targeted Therapy2022，7，12： | 0 |
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