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| 1 | Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism显示文摘Amycolatopsis mediterranei 被用于 rifamycin 的工业规模生产,它在 antimycobacterial 治疗起一个重要作用。作为类 Amycolatopsis 的首先定序的染色体, 236 715 底配对的包括 10 的紧张 U32 的染色体,最大的原核生物的染色体之一到目前为止曾经被定序。不同于在 streptomycetes 发现的线性拓扑学,这个染色体是圆形的,特别地类似于 Saccharopolyspora erythraea 和奴卡氏菌属 farcinica,在发展史和分类代表他们的靠近的关系。尽管在 A 预言了 9 228 编码蛋白质的基因。mediterranei 染色体 S 与那些分享了 orthologs 的最大的数字。erythraea,它被 Streptomyces coelicolor 而非 N 出人意料地跟随。farcinica,显示不同新陈代谢的特征经由改编演变到多样的生态的壁龛。除类似于那的一个核心区域以外在 streptomycetes 普通,有典型核心特征的一个新奇“伪核心”在非核心区域以内被定义,在 21 从 26 基因为第二等的代谢物生产聚类的总数被定位的地方。位于核心的 rifamycin 生合成基因簇为 rifamycin SV 的变换编码细胞色素 P450 酶必需品到 B,由比作 rifamycin 生产 B 紧张 S699 的高度相应的簇揭示了并且进一步由基因互补证实了。A 的 genomic 信息。mediterranei 表明在看起来复杂的规章的机制的控制下面不仅为各种各样的碳来源和无机的氮混合物的广泛的利用而且为新陈代谢的中介的有效 funneling 安排进第二等的抗菌素合成进程的一个新陈代谢的网络。 | Wei Zhao Yi Zhong Hua Yuan Jin Wang Huajun Zheng Ying Wang Xufeng Cen Feng Xu Jie Bai Xiaobiao Han Gang Lu Yongqiang Zhu Zhihui Shao Han Yan Chen Li Nanqiu Peng Zilong Zhang Yunyi Zhang Wei Lin Yun Fan Zhongjun Qin Yongfei Hu Baoli Zhu Shengyue Wang Xiaoming Ding Guo-Ping Zbao | 2010 | Cell Research2010，20，10： | 10 |
| 2 | Improvement of oxytetracycline production mediated via cooperation of resistance genes in Streptomyces rimosus显示文摘Increasing the self-resistance levels of Streptomyces is an effective strategy to improve the production of antibiotics.To increase the oxytetracycline(OTC) production in Streptomyces rimosus,we investigated the cooperative effect of three co-overexpressing OTC resistance genes:one gene encodes a ribosomal protection protein(otrA) and the other two express efflux proteins(otrB and otrC).Results indicated that combinational overexpression of otrA,otrB,and otrC(MKABC) exerted a synergetic effect.OTC production increased by 179%in the recombinant strain compared with that of the wild-type strain M4018.The resistance level to OTC was increased by approximately two-fold relative to the parental strain,thereby indicating that applying the cooperative effect of self-resistance genes is useful to improve OTC production.Furthermore,the previously identified cluster-situated activator OtcR was overexpressed in MKABC in constructing the recombinant strain MKRABC;such strain can produce OTC of approximately7.49 g L^((-1)),which represents an increase of 19%in comparison with that of the OtcR-overexpressing strain alone.Our work showed that the cooperative overexpression of self-resistance genes is a promising strategy to enhance the antibiotics production in Streptomyces. | Shouliang Yin Xuefeng Wang Mingxin Shi Fang Yuan Huizhuan Wang Xiaole Jia Fang Yuan Jinliang Sun Tiejun Liu Keqian Yang Yuxiu Zhang Keqiang Fan Zilong Li | 2017 | Science China(Life Sciences)2017，60，9： | 10 |
| 3 | Cellular microparticles and pathophysiolog, of traumatic brain injury显示文摘 | Zilong Zhao Yuan Zhou Ye Tian Min Li Jing-fei Dong Jianning Zhang | 2017 | Protein & Cell2017，8，11： | 9 |
| 4 | Towards the Framework of Understanding Autism Spectrum Disorders显示文摘The prevalence of autism spectrum disorders(ASD)has been high worldwide,reaching 1/59 children in the United States as reported by the Centers of Disease Control and Prevention.Since genetic components play a major role in ASD[1],it is astonishing that the occurrence of ASD would be this high probabily due to genetic causes.It is worthy to note that autistic phenotypes of ASD patients show great diversity.The severity of autistic symptoms may be correlated with whether genetic mutations affect neural development.Thus,we argue that the prevalence of severe ASD may be much lower than the common ASD usually reported. | Zilong Qiu Bo Yuan | 2019 | Neuroscience Bulletin2019，35，6： | 5 |
| 5 | PhaseⅡstudy of induction chemotherapy followed by concurrent chemoradiotherapy with raltitrexed and cisplatin in locally advanced nasopharyngeal carcinoma显示文摘Objective:For locally advanced nasopharyngeal carcinoma(LA-NPC)patients,high incidences of distant metastases and severe treatment related toxicities are the main obstacles needed to be overcome.Raltitrexed,a specific thymidylate synthase inhibitor with a convenient administration schedule,has an acceptable and manageable toxicity,and possesses radio-sensitizing properties.To investigate the efficacy and safety of raltitrexed and cisplatin induction chemotherapy and concurrent chemoradiotherapy(IC+CCRT)in patients with LA-NPC,a phaseⅡclinical study was conducted.Methods:Sixty eligible patients with LA-NPC were enrolled into this study.A raltitrexed-cisplatin combination was used as part of an IC+CCRT regimen.Raltitrexed-cisplatin IC was given once every 3 weeks(q3 w)for two cycles,followed by raltitrexed-cisplatin based CCRT q3 w for two cycles.Intensity-modulated radiotherapy(IMRT)was given for all enrolled patients.Results:All patients were included in survival analysis according to the intent-to-treat principle.The objective response rate(ORR)3 months after treatment was 98%.The 2-year overall survival(OS)rate was 92%.The median relapse-free survival(RFS)time was 30.5[95%confidence interval(95%CI),28.4-32.3]months.The 2-year RFS rate was 85%.The 2-year local failure-free survival(LFFS)rate was 97%and the 2-year distant metastasis-free survival(DMFS)rate was 88%.Acute toxicities were mostly grade 2 and 3 reactions in bone marrow suppression,gastrointestinal side effect and oropharyngeal mucositis.Only two patients occurred grade 4 acute toxicities,one was bone marrow suppression and the other was dermatitis radiation.Conclusions:The combination of raltitrexed and cisplatin has a comparable efficacy to those in standard firstline therapy. | Yuan Wu Xueyan Wei Zilong Yuan Hongbin Xu Yanping Li Ying Li Liu Hu Guang Han Yu Qian Desheng Hu | 2020 | Chinese Journal of Cancer Research2020，32，5： | 4 |
| 6 | OFDM Based Bidirectional Multi-Relay SWIPT Strategy for 6G IoT Networks显示文摘6G IoT networks aim for providing significantly higher data rates and extremely lower latency.However,due to the increasingly scarce spectrum bands and ever-growing massive number IoT devices(IoDs)deployed,6G IoT networks face two critical challenges,i.e.,energy limitation and severe signal attenuation.Simultaneous wireless information and power transfer(SWIPT)and cooperative relaying provide effective ways to address these two challenges.In this paper,we investigate the energy self-sustainability(ESS)of 6G IoT network and propose an OFDM based bidirectional multi-relay SWIPT strategy for 6G IoT networks.In the proposed strategy,the transmission process is equally divided into two phases.Specifically,in phase1 two source nodes transmit their signals to relay nodes which will then use different subcarrier sets to decode information and harvest energy,respectively.In phase2 relay nodes forward signals to corresponding destination nodes with the harvested energy.We maximize the weighted sum transmission rate by optimizing subcarriers and power allocation.Our proposed strategy achieves larger weighted sum transmission rate comparing with the benchmark scheme. | Weidang Lu Peiyuan Si Xin Liu Bo Li Zilong Liu Nan Zhao Yuan Wu | 2020 | China Communications2020，17，12： | 1 |
| 7 | Engineer Nanoscale Defects into Selective Channels:MOF-Enhanced Li^(+) Separation by Porous Layered Double Hydroxide Membrane显示文摘Two-dimensional(2D)membrane-based ion separation technology has been increasingly explored to address the problem of lithium resource shortage,yet it remains a sound challenge to design 2D membranes of high selectivity and permeability for ion separation applications.Zeolitic imidazolate framework functionalized modified layered double hydroxide(ZIF-8@MLDH)composite membranes with high lithium-ion(Li^(+)) permeability and excellent operational stability were obtained in this work by in situ depositing functional ZIF-8 nanoparticles into the nanopores acting as framework defects in MLDH membranes.The defect-rich framework amplified the permeability of Li^(+),and the site-selective growth of ZIF-8 in the framework defects bettered its selectivity.Specifically speaking,the ZIF-8@MLDH membranes featured a high permeation rate of Li^(+) up to 1.73 mol m^(−2) h^(−1) and a desirable selectiv-ity of Li^(+)/Mg^(2+) up to 31.9.Simulations supported that the simultaneously enhanced selectivity and permeability of Li+are attributed to changes in the type of mass transfer channels and the difference in the dehydration capacity of hydrated metal cations when they pass through nanochannels of ZIF-8.This study will inspire the ongoing research of high-performance 2D membranes through the engineering of defects. | Yahua Lu Rongkun Zhou Naixin Wang Yuye Yang Zilong Zheng Miao Zhang Quan-Fu An Jiayin Yuan | 2023 | Nano-Micro Letters2023，15，9： | 1 |
| 8 | An Intronic Variant of CHD7 Identified in Autism Patients Interferes with Neuronal Differentiation and Development显示文摘Genetic composition plays critical roles in the pathogenesis of autism spectrum disorder(ASD).Especially,inherited and de novo intronic variants are often seen in patients with ASD.However,the biological significance of intronic variants is difficult to address.Here,among a Chinese ASD cohort,we identified a recurrent inherited intronic variant in the CHD7 gene,which is specifically enriched in East Asian populations.CHD7 has been implicated in numerous developmental disorders including CHARGE syndrome and ASD.To investigate whether the ASD-associated CHD7 intronic variant affects neural development,we established human embryonic stem cells carrying this variant using CRISPR/Cas9 methods and found that the level of CHD7 mRNA significantly decreased compared to control.Upon differentiation towards the forebrain neuronal lineage,we found that neural cells carrying the CHD7 intronic variant exhibited developmental delay and maturity defects.Importantly,we found that TBR1,a gene also implicated in ASD,was significantly increased in neurons carrying the CHD7 intronic variant,suggesting the intrinsic relevance among ASD genes.Furthermore,the morphological defects found in neurons carrying CHD7 intronic mutations were rescued by knocking down TBR1,indicating that TBR1 may be responsible for the defects in CHD7-related disorders.Finally,the CHD7 intronic variant generated three abnormal forms of transcripts through alternative splicing,which all exhibited loss-of-function in functional assays.Our study provides crucial evidence supporting the notion that the intronic variant of CHD7 is potentially an autism susceptibility site,shedding new light on identifying the functions of intronic variants in genetic studies of autism. | Ran Zhang Hui He Bo Yuan Ziyan Wu Xiuzhen Wang Yasong Du Yuejun Chen Zilong Qiu | 2021 | Neuroscience Bulletin2021，37，8： | 1 |
| 9 | Electrocatalytic properties of carbon nanotubes supported ternary PtSnIn catalysts for ethanol electro-oxidation显示文摘 | Daobao Chu Zilong Li Ximei Yuan Jian Li Xu Wei Yong Wan | 2012 | Electrochimica Acta2012，，： | 1 |
| 10 | Middle Cambrian to Permian subduction-related accretionary orogenesis of Northern Xinjiang, NW China: Implications for the tectonic evolution of central Asia显示文摘 | Wenjiao Xiao Chunming Han Chao Yuan Min Sun Shoufa Lin Hanlin Chen Zilong Li Jiliang Li Shu Sun | 2007 | Journal of Asian Earth Sciences2007，，2： | 1 |
| 11 | Middle Cambrian to Permian subduction-related accretionary orogenesis of Northern Xinjiang, NW China: Implications for the tectonic evolution of central Asia显示文摘 | Wenjiao Xiao Chunming Han Chao Yuan Min Sun Shoufa Lin Hanlin Chen Zilong Li Jiliang Li Shu Sun | 2007 | Journal of Asian Earth Sciences2007，，2： | 1 |
| 12 | Dual-crosslinked regenerative hydrogel for sutureless long-term repair of corneal defect显示文摘Corneal transplantation is the most effective clinical treatment for corneal defects,but it requires precise size of donor corneas,surgical sutures,and overcoming other technical challenges.Postoperative patients may suffer graft rejection and complications caused by sutures.Ophthalmic glues that can long-term integrate with the corneal tissue and effectively repair the focal corneal damage are highly desirable.Herein,a hybrid hydrogel consisting of porcine decellularized corneal stroma matrix(pDCSM)and methacrylated hyaluronic acid(HAMA)was developed through a non-competitive dual-crosslinking process.It can be directly filled into corneal defects with various shapes.More importantly,through formation of interpenetrating network and stable amide bonds between the hydrogel and adjacent tissue,the hydrogel manifested excellent adhesion properties to achieve suture-free repair.Meanwhile,the hybrid hydrogel not only preserved bioactive components from pDCSM,but also exhibited cornea-matching transparency,low swelling ratio,slow degradation,and enhanced mechanical properties,which was capable of withstanding superhigh intraocular pressure.The combinatorial hydrogel greatly improved the poor cell adhesion performance of HAMA,supported the viability,proliferation of corneal cells,and preservation of keratocyte phenotype.In a rabbit corneal stromal defect model,the experimental eyes treated with the hybrid hydrogel remained transparent and adhered intimately to the stroma bed with long-term retention,accelerated corneal re-epithelialization and wound healing.Giving the advantages of high bioactivity,low-cost,and good practicality,the dual-crosslinked hybrid hydrogel served effectively for long-term suture-free treatment and tissue regeneration after corneal defect. | Xuanren Shen Saiqun Li Xuan Zhao Jiandong Han Jiaxin Chen Zilong Rao Kexin Zhang Daping Quan Jin Yuan Ying Bai | 2023 | Bioactive Materials2023，，2： | 1 |
| 13 | Middle Cambrian to Permian subduction-related accretionary orogenesis of Northern Xinjiang, NW China: Implications for the tectonic evolution of central Asia 显示文摘 | Xiao Wenjiao Han Chunming Yuan Chao Sun Min Lin Shoufa Chen Hanlin Li Zilong Li Jiliang Sun Shu | 2008 | Journal of Asian Earth Sciences2008，32，： | 1 |
| 14 | Autism-related protein MeCP2 regulates FGF13 expression and emotional behaviors显示文摘Methyl-CpG binding protein 2(MeCP2)has a crucial role in transcriptional regulation and neural development(Ausio et al.,2014).Loss of function mutations of MECP2 in human lead to Rett syndrome(RTT),a severe neurodevelopmental disorders(Amir et al.,1999),whereas individuals with the chromosomal | BO Yuan Tian-lin Cheng Kan Yang Xu Zhang Zilong Qiu | 2017 | Journal of Genetics and Genomics2017，44，1： | 1 |
| 15 | Single-dose AAV-based vaccine induces a high level of neutralizing antibodies against SARS-CoV-2 in rhesus macaques显示文摘Dear Editor,Coronavirus disease 2019(COVID-19)is a highly infectious respiratory disease that continues to pose a serious global public health emergency.The disease shows a high infection rate,long incubation period,and rapidly emerging variants,which have led to its rapid spread worldwide(Krammer 2020).Many vaccines have been developed for the control of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the virus responsible for COVID-19,including vaccines based on messenger RNA(mRNA)(Polack et al.2020),viral vectors(Zhu et al.2020),recombinant proteins(Yang et al.2020),and inactivated SARS-CoV-2(Zhang et al.2021). | Dali Tong Mei Zhang Yunru Yang Han Xia Haiyang Tong Huajun Zhang Weihong Zeng Muziying Liu Yan Wu Huan Ma Xue Hu Weiyong Liu Yuan Cai Yanfeng Yao Yichuan Yao Kunpeng Liu Shifang Shan Yajuan Li Ge Gao Weiwei Guo Yun Peng Shaohong Chen Juhong Rao Jiaxuan Zhao Juan Min Qingjun Zhu Yanmin Zheng Lianxin Liu Chao Shan Kai Zhong Zilong Qiu Tengchuan Jin Sandra Chiu Zhiming Yuan Tian Xue | 2023 | Protein & Cell2023，14，1： | 0 |
| 16 | Robust zero-watermarking algorithm based on discrete wavelet transform and daisy descriptors for encrypted medical image显示文摘In the intricate network environment,the secure transmission of medical images faces challenges such as information leakage and malicious tampering,significantly impacting the accuracy of disease diagnoses by medical professionals.To address this problem,the authors propose a robust feature watermarking algorithm for encrypted medical images based on multi-stage discrete wavelet transform(DWT),Daisy descriptor,and discrete cosine transform(DCT).The algorithm initially encrypts the original medical image through DWT-DCT and Logistic mapping.Subsequently,a 3-stage DWT transformation is applied to the encrypted medical image,with the centre point of the LL3 sub-band within its low-frequency component serving as the sampling point.The Daisy descriptor matrix for this point is then computed.Finally,a DCT transformation is performed on the Daisy descriptor matrix,and the low-frequency portion is processed using the perceptual hashing algorithm to generate a 32-bit binary feature vector for the medical image.This scheme utilises cryptographic knowledge and zero-watermarking technique to embed watermarks without modifying medical images and can extract the watermark from test images without the original image,which meets the basic re-quirements of medical image watermarking.The embedding and extraction of water-marks are accomplished in a mere 0.160 and 0.411s,respectively,with minimal computational overhead.Simulation results demonstrate the robustness of the algorithm against both conventional attacks and geometric attacks,with a notable performance in resisting rotation attacks. | Yiyi Yuan Jingbing Li Jing Liu Uzair Aslam Bhatti Zilong Liu Yen-wei Chen | 2024 | CAAI Transactions on Intelligence Technology2024，9，1： | 0 |
| 17 | Targeted deep sequencing reveals the genetic heterogeneity in well-differentiated pancreatic neuroendocrine tumors with liver metastasis显示文摘Background:Pancreatic neuroendocrine tumor is a rare and heterogeneous entity,and approximately half of the patients harbored liver metastasis when initially diagnosed,whose prognosis is dismal.High-throughput sequencing has largely uncovered the genomic features of pancreatic neuroendocrine tumor,but the genetic alterations in the metastatic cases remain relatively unclear,which we aimed to study.Methods:Pathologically confirmed well-differentiated pancreatic neuroendocrine tumor samples resected in our hospital from 2000 to 2019 were collected.We performed deep sequencing on the exome of 341 tumor-related genes,and compared the differences of genetic alterations between the metastatic and the non-metastatic cases,as well as between the primary and the paired liver metastatic tumors.Results:Sequencing data of 79 samples from 29 pancreatic neuroendocrine tumor patients were included into analysis.A total of 2,471 somatic variants were identified,75.5%of which were considered as low-abundance.NOTCH1 was the most frequently mutated gene,altered in 26(53.1%)pancreatic neuroendocrine tumor samples from 18(62.1%)patients.Compared with the non-metastatic pancreatic neuroendocrine tumors,the metastatic cases were discovered with more single nucleotide variants and copy number variations,indicating the increased genomic instability.In addition,among the paired metastatic cases,the primary and the metastatic lesions shared limited mutated genes.Conclusions:Through the targeted deep sequencing,we identified the intratumor,intraindividual,and interindividual heterogeneity in the pancreatic neuroendocrine tumor patients,particularly in the metastatic cases,bringing potential challenges for the current biopsy strategies in guiding clinical treatments. | Wentao Zhou Xu Han Yuan Ji Dansong Wang Dong Xie Zilong Qiu Wenhui Lou | 2023 | Hepatobiliary Surgery and Nutrition2023，12，3： | 0 |
| 18 | Prompt learning in computer vision: a survey显示文摘Prompt learning has attracted broad attention in computer vision since the large pre-trained visionlanguagemodels (VLMs) exploded. Based on the close relationship between vision and language information builtby VLM, prompt learning becomes a crucial technique in many important applications such as artificial intelligencegenerated content (AIGC). In this survey, we provide a progressive and comprehensive review of visual promptlearning as related to AIGC. We begin by introducing VLM, the foundation of visual prompt learning. Then, wereview the vision prompt learning methods and prompt-guided generative models, and discuss how to improve theefficiency of adapting AIGC models to specific downstream tasks. Finally, we provide some promising researchdirections concerning prompt learning. | Yiming LEI Jingqi LI Zilong LI Yuan CAO Hongming SHAN | 2024 | Frontiers of Information Technology & Electronic Engineering2024，25，1： | 0 |
| 19 | Identification of de novo Mutations in the Chinese Autism Spectrum Disorder Cohort via Whole-Exome Sequencing Unveils Brain Regions Implicated in Autism显示文摘Autism spectrum disorder(ASD)is a highly heritable neurodevelopmental disorder characterized by deficits in social interactions and repetitive behaviors.Although hundreds of ASD risk genes,implicated in synaptic formation and transcriptional regulation,have been identified through human genetic studies,the East Asian ASD cohorts are still under-represented in genome-wide genetic studies.Here,we applied whole-exome sequencing to 369 ASD trios including probands and unaffected parents of Chinese origin.Using a joint-calling analytical pipeline based on GATK toolkits,we identified numerous de novo mutations including 55 high-impact variants and 165 moderate-impact variants,as well as de novo copy number variations containing known ASD-related genes.Importantly,combined with single-cell sequencing data from the developing human brain,we found that the expression of genes with de novo mutations was specifically enriched in the pre-,post-central gyrus(PRC,PC)and banks of the superior temporal(BST)regions in the human brain.By further analyzing the brain imaging data with ASD and healthy controls,we found that the gray volume of the right BST in ASD patients was significantly decreased compared to healthy controls,suggesting the potential structural deficits associated with ASD.Finally,we found a decrease in the seed-based functional connectivity between BST/PC/PRC and sensory areas,the insula,as well as the frontal lobes in ASD patients.This work indicated that combinatorial analysis with genome-wide screening,single-cell sequencing,and brain imaging data reveal the brain regions contributing to the etiology of ASD. | Bo Yuan Mengdi Wang Xinran Wu Peipei Cheng Ran Zhang Ran Zhang Shunying Yu Jie Zhang Yasong Du Xiaoqun Wang Zilong Qiu | 2023 | Neuroscience Bulletin2023，39，10： | 0 |
| 20 | 诱导性多能干细胞的低基因组稳定性使非同源末端连接增加显示文摘背景与目的诱导性多能干细胞(induced pluripotent stem cells,iPSCs)和胚胎干细胞(embryonic stem cells,ESCs)具有许多共同特征,包括相似的形态、基因表达和体外分化谱。然而,iPSCs的基因组稳定性远低于ESCs。在本研究中,我们研究了iPSCs中DNA损伤修复的改变是否为其具有更大诱变倾向的原因。方法将小鼠iPSCs、ESCs和胚胎成纤维细胞暴露于电离辐射(4 Gy),导致双链DNA断裂。照射4 h后使用全基因组重测序评估DNA损伤修复的保真度。我们还分析了分别源自iPSCs或ESCs的小鼠的基因组稳定性。结果照射后,与胚胎干细胞和胚胎成纤维细胞相比,iPSCs具有较低的DNA损伤修复能力,有更多的体细胞突变和短片段插入缺失。iPSCs有更多的非同源末端连接DNA修复和更少的同源重组DNA修复。源自iPSCs的小鼠比ESCs小鼠以及C57对照小鼠的DNA损伤修复能力更低。结论本研究结果部分表明,iPSCs的低基因组稳定性及其在体内的高致瘤性是由DNA损伤修复的低保真度所致。 | Minjie Zhang Liu Wang Ke An Jun Cai Guochao Li Caiyun Yang Huixian Liu Fengxia Du Xiao Han Zilong Zhang Zitong Zhao Duanqing Pei Yuan Long Xin Xie Qi Zhou Yingli Sun | 2019 | 癌症2019，38，8： | 0 |
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