|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Andrographolide sulfonate ameliorates lipopolysaccharide-induced acute lung injury in mice by down-regulating MAPK and NF-κB pathways显示文摘Acute lung injury(ALI) or acute respiratory distress syndrome(ARDS) is a severe, lifethreatening medical condition characterized by widespread inflammation in the lungs, and is a significant source of morbidity and mortality in the patient population. New therapies for the treatment of ALI are desperately needed. In the present study, we examined the effect of andrographolide sulfonate, a water-soluble form of andrographolide(trade name: Xi-Yan-Ping Injection), on lipopolysaccharide(LPS)-induced ALI and inflammation. Andrographolide sulfonate was administered by intraperitoneal injection to mice with LPS-induced ALI. LPS-induced airway inflammatory cell recruitment and lung histological alterations were significantly ameliorated by andrographolide sulfonate. Protein levels of pro-inflammatory cytokines in bronchoalveolar lavage fluid(BALF) and serum were reduced by andrographolide sulfonate administration. m RNA levels of pro-inflammatory cytokines in lung tissue were also suppressed. Moreover, andrographolide sulfonate markedly suppressed the activation of mitogen-activated protein kinase(MAPK) as well as p65 subunit of nuclear factor-κB(NF-κB). In summary, these results suggest that andrographolide sulfonate ameliorated LPS-induced ALI in mice by inhibiting NF-κB and MAPK-mediated inflammatory responses. Our study shows that water-soluble andrographolide sulfonate may represent a new therapeutic approach for treating inflammatory lung disorders. | Shuang Peng Nan Hang Wen Liu Wenjie Guo Chunhong Jiang Xiaoling Yang Qiang Xu Yang Sun | 2016 | Acta Pharmaceutica Sinica B2016，6，3： | 38 |
| 2 | Viral suppression of RNA silencing显示文摘Gene silencing (RNA silencing) plays a fundamental role in antiviral defense in plants, fungi and invertebrates. Viruses encode proteins that suppress gene silencing to counter host defense. Viral suppressors of RNA silencing (VSRs) have been identified from almost all plant virus genera and some viruses of insects and mammals. Recent studies have revealed that VSRs counter host defense and interfere with host gene regulation by interacting with RNA or important components of the RNA silencing pathway. Here, we review the current understanding of the complex mechanisms of VSRs that have been revealed by recent studies. | JIANG Lin WEI ChunHong LI Yi | 2012 | Science China(Life Sciences)2012，55，2： | 8 |
| 3 | Sediment delivery ratio and its uncertainties on flood event scale:Quantification for the Lower Yellow River显示文摘Sediment delivery ratio(SDR)for fluvial rivers was formulated with sediment rating curve.The observed data of SDR on flood event scale of the Lower Yellow River(LYR)were adopted to examine the formulation and to calibrate the model parameters.A regression formula of SDR was then established and its 95%prediction interval was accordingly quantified to represent its overall uncertainties.Three types of factors including diversity of the incoming flow conditions,river self-regulation processes,and human activities were ascribed to the uncertainties.The following were shown:(1)With the incoming sediment coefficient(ISC)being a variable,it was not necessary to adopt the incoming flow discharge as the second variable in the formulation of SDR;and(2)ISC=0.003 and therefore SDR=2 might be a threshold for distinguishing the characteristics of sediment transport within the LYR.These findings would highlight sediment transport characteristics on the scale of flood event and contribute to uncertainty based analysis of water volume required for sediment transport and channel maintenance of the LYR. | FU XuDong 1,JIANG LiWei 1,WU BaoSheng 1,HU ChunHong 2,WANG GuangQian 1 &FEI XiangJun 1 1State Key Laboratory of Hydroscience and Engineering,Tsinghua University,Beijing 100084,China 2China Institute of Hydropower and Water Resources Research,Beijing 100044,China | 2010 | Science China(Technological Sciences)2010，53，3： | 7 |
| 4 | Reduced graphene oxide@carbon sphere based metacomposites for temperature-insensitive and efficient microwave absorption显示文摘With the increasing advance of fifth generation(5G)network and the gradual expansion of digital devices,harsh working environment for electronic devices has spawned higher requirements for microwave absorbing materials(MAMs).Since both the electromagnetic response and energy conversion character vary with temperature,to achieve temperature insensitive microwave absorption behaviour in wide temperature range has become extremely challenging.In this work,structured metacomposites containing sub-wavelength reduced graphene oxide(RGO)@carbon spheres were fabricated,and the microwave absorption was further improved through structural and composition design of the RGO@carbon units.Due to the unique anti-reflection effect on microwave of the metacomposites,the temperature-insensitive electromagnetic performance at elevated temperature could be exhibited.Moreover,both the dielectric relaxation behaviour and microwave absorption proformance of the system could be further increased.As a result,the effective absorption bandwidth(reflection loss(RL)<−10 dB)of the metacomposites with only 3.0 wt.%filler content could cover the entire X-band(8.2–12.4 GHz)frequency ranging from 298 to 473K.The metacomposite proposed in this work provides a“de-correlating”strategy to break the linkage between microwave absorption behaviour and temperature,which offers an interesting plateau for fabricating efficient high-temperature microwave absorption structures with tunable and designable advantages. | Zhiyang Jiang Haoxu Si Yi Li Dan Li Huihui Chen Chunhong Gong Jingwei Zhang | 2022 | Nano Research2022，15，9： | 4 |
| 5 | How Microbes Shape Their Communities? A Microbial Community Model Based on Functional Genes显示文摘Exploring the mechanisms of maintaining microbial community structure is important to understand biofilm development or microbiota dysbiosis. In this paper, we propose a functional gene-based composition prediction(FCP) model to predict the population structure composition within a microbial community. The model predicts the community composition well in both a low-complexity community as acid mine drainage(AMD) microbiota, and a complex community as human gut microbiota. Furthermore, we define community structure shaping(CSS) genes as functional genes crucial for shaping the microbial community. We have identified CSS genes in AMD and human gut microbiota samples with FCP model and find that CSS genes change with the conditions. Compared to essential genes for microbes, CSS genes are significantly enriched in the genes involved in mobile genetic elements, cell motility, and defense mechanisms, indicating that the functions of CSS genes are focused on communication and strategies in response to the environment factors. We further find that it is the minority, rather than the majority, which contributes to maintaining community structure. Compared to health control samples, we find that some functional genes associated with metabolism of amino acids, nucleotides, and lipopolysaccharide are more likely to be CSS genes in the disease group. CSS genes may help us to understand critical cellular processes and be useful in seeking addable gene circuitries to maintain artificial self-sustainable communities. Our study suggests that functional genes are important to the assembly of microbial communities. | Xiaoqing Jiang Xin Li Longshu Yang Chunhong Liu Qi Wang Weilai Chi Huaiqiu Zhu | 2019 | Genomics, Proteomics & Bioinformatics2019，17，1： | 3 |
| 6 | TiN/BN composite with excellent thermal stability for efciency microwave absorption in wide temperature spectrum显示文摘High-efficiency and temperature-insensitive microwave absorbing materials(MAMs) are ideal for hightemperature electromagnetic attenuation.Herein,the titanium nitride/boron nitride(TiN/BN) composite with TiN as the loss unit and BN as the impedance matching unit has been constructed by the in-situ synthesis method.The insulating BN not only effectively regulates and optimizes the conductivity and impedance matching of the material but also imparts steady cyclic microwave absorption properties.The steady dielectric loss ensures that TiN/BN composite has robust high-temperature absorption properties at X-band,with a minimum reflection loss(RL_(min)) of-16.74 dB at 873 K and an effective absorption bandwidth(EAB) of 3.26 GHz in the temperature range of 293-873 K.Compared with the TiN/SiO2absorbers with a single system,the TiN/BN/SiO2composite keeps reliable high temperature absorbing properties at873 K.This work confirms that wave-transparent materials for dielectric property modulation can resolve poor temperature stability of effective absorption and non-reusability for high-temperature absorbing materials,providing inspirations for designing efficiency high-temperature microwave absorbers. | Yupeng Shi Dan Li Haoxu Si Zhiyang Jiang Mengyuan Li Chunhong Gong | 2022 | Journal of Materials Science & Technology2022，，35： | 3 |
| 7 | OTUD5 promotes innate antiviral and antitumor immunity through deubiquitinating and stabilizing STING显示文摘Stimulator of interferon genes(STING)is an adaptor protein that is critical for effective innate antiviral and antitumor immunity.The activity of STING is heavily regulated by protein ubiquitination,which is fine-tuned by both E3 ubiquitin ligases and deubiquitinases.Here,we report that the deubiquitinase OTUD5 interacts with STING,cleaves its K48-linked polyubiquitin chains,and promotes its stability.Consistently,knockout of OTUD5 resulted in faster turnover of STING and subsequently impaired type I IFN signaling following cytosolic DNA stimulation.More importantly,Lyz2-Cre Otud5^(fl/Y) mice and CD11-Cre Otud5^(fl/Y) mice showed more susceptibility to herpes simplex virus type 1(HSV-1)infection and faster development of melanomas than their corresponding control littermates,indicating that OTUD5 is indispensable for STING-mediated antiviral and antitumor immunity.Our data suggest that OTUD5 is a novel checkpoint in the cGAS-STING cytosolic DNA sensing pathway. | Yunyun Guo Fei Jiang Lingli Kong Haifeng Wu Honghai Zhang Xiaorong Chen Jian Zhao Baoshan Cai Yanqi Li Chunhong Ma Fan Yi Lei Zhang Bingyu Liu Yi Zheng Lingqiang Zhang Chengjiang Gao | 2021 | Cellular & Molecular Immunology2021，18，8： | 2 |
| 8 | CUL4B facilitates HBV replication by promoting HBx stabilization显示文摘Objective:Hepatitis B virus(HBV)infection is a major public health problem worldwide.However,the regulatory mechanisms underlying HBV replication remain unclear.Cullin 4 B-RING ubiquitin E3 ligase(CRL4 B)is involved in regulating diverse physiological and pathophysiological processes.In our study,we aimed to explain the role of CUL4 B in HBV infection.Methods:Cul4 b transgenic mice or conditional knockout mice,as well as liver cell lines with CUL4 B overexpression or knockdown,were used to assess the role of CUL4 B in HBV replication.Immunoprecipitation assays and immunofluorescence staining were performed to study the interaction between CUL4 B and HBx.Cycloheximide chase assays and in vivo ubiquitination assays were performed to evaluate the half-life and the ubiquitination status of HBx.Results:The hydrodynamics-based hepatitis B model in Cul4 b transgenic or conditional knockout mice indicated that CUL4 B promoted HBV replication(P<0.05).Moreover,the overexpression or knockdown system in human liver cell lines validated that CUL4 B increased HBV replication in an HBx-dependent manner.Importantly,immunoprecipitation assays and immunofluorescence staining showed an interaction between CUL4 B and HBx.Furthermore,CUL4 B upregulated HBx protein levels by inhibiting HBx ubiquitination and proteasomal degradation(P<0.05).Finally,a positive correlation between CUL4 B expression and HBV pg RNA level was observed in liver tissues from HBV-positive patients and HBV transgenic mice.Conclusions:CUL4 B enhances HBV replication by interacting with HBx and disrupting its ubiquitin-dependent proteasomal degradation.CUL4 B may therefore be a potential target for anti-HBV therapy. | Haixia Shan Bo Wang Xiaodong Zhang Hui Song Xi Li Yongxin Zou Baichun Jiang Huili Hu Hao Dou Changshun Shao Lifen Gao Chunhong Ma Xiaoyun Yang Xiaohong Liang Yaoqin Gong | 2022 | Cancer Biology & Medicine2022，19，1： | 2 |
| 9 | CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription显示文摘Toll-like receptors (TLRs) play critical roles in innate immunity and inflammation. The molecular mechanisms by which TLR signaling is fine-tuned remain to be completely elucidated. Cullin 4B (CUL4B), which assembles the CUL4B-RING E3 ligase complex (CRL4B), has been shown to regulate diverse developmental and physiological processes by catalyzing monoubiquitination for histone modification or polyubiquitination for proteasomal degradation. Here, we identified the role of CUL4B as an intrinsic negative regulator of the TLR-triggered inflammatory response. Deletion of CUL4B in macrophages increased the production of proinflammatory cytokines and decreased anti-inflammatory cytokine IL-10 production in response to pathogens that activate TLR3, TLR4, or TLR2. Myeloid cell-specific Cul4b knockout mice were more susceptible to septic shock when challenged with lipopolysaccharide, polyinosinic-polycytidylic acid or Salmonella typhimurium infection. We further demonstrated that enhanced TLR-induced inflammatory responses in the absence of CUL4B were mediated by increased GSK3β activity. Suppression of GSK3β activity efficiently blocked the TLR-triggered increase in proinflammatory cytokine production and attenuated TLR-triggered death in Cul4b mutant mice. Mechanistically, CUL4B was found to negatively regulate TLR-triggered signaling by epigenetically repressing the transcription of Pten, thus maintaining the anti-inflammatory PI3K-AKT-GSK3β pathway. The upregulation of PTEN caused by CUL4B deletion led to uncontrolled GSK3β activity and excessive inflammatory immune responses. Thus, our findings indicate that CUL4B functions to restrict TLR-triggered inflammatory responses through regulating the AKT-GSK3β pathway. | Yu Song Peishan Li Liping Qin Zhiliang Xu Baichun Jiang Chunhong Ma Changshun Shao Yaoqin Gong | 2021 | Cellular & Molecular Immunology2021，18，2： | 2 |
| 10 | DNA methylation and alternative splicing modulate FBXW11 gene expression in Holstein bull testis and are correlated with sperm quality显示文摘F-box and WD-40 domain protein 11(FBXW11)is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of spermatogenesis.In our previous research,the mRNA expression of FBXW11 in bull sperm with high motility is significantly higher than that with low motility.In the present study,the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance groups.The immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous tubules.Three splice variants of the FBXW11 gene,namely,FBXW11-tv1,FBXW11-tv2,and FBXW11-tv3,were identified in testicular tissues.The splicing patterns of the three variants are exon skipping.The transcript FBXW11-tv2 expressions were the highest in each sample.The low-performance groups displayed higher FBXW11-tv1 and FBXW11-tv2 transcript expressions than the normal performance groups.Two CpG islands were located within the 5’UTR and exon 1-2 region of the FBXW11 gene.Bisulfite sequencing PCR results demonstrated that the methylation levels of 11 methylation sites in the CpG island 2 from−99 to−43 in the normal performance groups were significantly lower than those in the low-performance groups.Pearson correlation analysis suggested that the CpG island 2 methylation level was negatively correlated with sperm motility and the transcript FBXW11-tv2 expression level.Our data revealed that alternative splicing and DNA methylation jointly regulated FBXW11 gene expression and were correlated with sperm quality traits during spermatogenesis in Holsteins. | YONG LIU ZHIHUA JU QIANG JIANG WENHAO LIU CHUNHONG YANG YARAN ZHANG XIUGE WANG YAPING GAO XIAOCHAO WEI YAN SUN JINPENG WANG MINGHAI HOU LING YANG JINMING HUANG | 2021 | BIOCELL2021，45，1： | 1 |
| 11 | Phytic acid conversion coating on Mg-Li alloy显示文摘 | Lib Gao Chunhong Zhang Milin Zhang Xiaomei Huang Xi Jiang | | 0，，： | 1 |
| 12 | Expression pattern and regulation of head-to-head genes Vps36 and Ckap2 during chicken follicle development显示文摘Vacuolar protein sorting 36(VPS36),a protein primarily known for its role in the Endosomal Sorting Complex Required for Transport pathway,has recently been shown to be linked to chicken reproduction.Previous research showed that Vps36 is significantly downregulated in sexually mature chicken ovaries compared to immature ones.In this study,using real-time quantitative RT-PCR,we investigated the expression pattern of Vps36 and its head-to-head gene Ckap2 mRNA in chicken follicles.Small white follicles were found to have significantly higher expression of Vps36 and Ckap2 mRNA than any other sized follicles(P<0.05).The expression of Vps36 and Ckap2 mRNA were detected in both granulosa and theca layers of pre-ovulatory follicles,the expression of Ckap2 in theca layers was slightly higher than in granulosa cells.Treatment of small yellow follicles with folliclestimulating hormone and estradiol resulted in a marked decrease of both Vps36 and Ckap2 mRNA(P<0.05);however,progesterone,transforming growth factor-β1 and luteinizing hormone induced no significant changes in Vps36 and Ckap2 mRNA expression in these follicles.These results indicate that the head-to-head genes of Vps36 and Ckap2 exhibit similar expression in chicken follicles and are involved in chicken follicle development. | Xinxing CUI Chunhong YANG Li KANG Guiyu ZHU Qingqing WEI Yunliang JIANG | 2014 | Frontiers of Agricultural Science and Engineering2014，1，2： | 1 |
| 13 | The universal formula computing the position of subaperture spot image for Shack-Hartmann wavefront sensor 显示文摘 | Huilun LIN Chunhong WANG Wenhan JIANG | 2004 | SPIE2004，5639，： | 1 |
| 14 | Paclitaxel liposome for injection (Lipusu) plus cisplatin versus gemcitabine plus cisplatin in the first-line treatment of locally advanced or metastatic lung squamous cell carcinoma: A multicenter, randomized, open-label, parallel controlled clinical study显示文摘Background:Lipusu is the first commercialized liposomal formulation of pacli-taxel and has demonstrated promising efficacy against locally advanced lung squamous cell carcinoma(LSCC)in a small-scale study.Here,we conducted a multicenter,randomized,phase 3 study to compare the efficacy and safety of cis-platin plus Lipusu(LP)versus cisplatin plus gemcitabine(GP)as first-line treat-ment in locally advanced or metastatic LSCC.Methods:Patients enrolled were aged between 18 to 75 years,had locally advanced(clinical stage IIIB,ineligible for concurrent chemoradiation or surgery)or metastatic(Stage IV)LSCC,had no previous systemic chemother-apy and at least one measurable lesion as per the Response Evaluation Criteria in Solid Tumors(version 1.1)before administration of the trial drug.The primary endpoint was progression-free survival(PFS).The secondary endpoints included objective response rate(ORR),disease control rate(DCR),overall survival(OS),and safety profiles.To explore the possible predictive value of plasma cytokines for LP treatment,plasma samples were collected from the LP group at baseline and first efficacy evaluation time and were then subjected to analysis by 45-Plex ProcartaPlex Panel 1 to detect the presence of 45 cytokines using the Luminex xMAP technology.The correlation between treatment outcomes and dynamic changes in the levels of cytokines were evaluated in preliminary analyses.Results:The median duration of follow-up was 15.4 months.237 patients in the LP group and 253 patients in the GP group were included in the per protocol set(PPS).In the PPS,the median PFS was 5.2 months versus 5.5 months in the LP and GP group(hazard rtio[HR]:1.03,P=0.742)respectively.The median OS was 14.6 months versus 12.5 months in the LP and GP group(HR:0.83,P=0.215).The ORR(41.8%versus 45.9%,P=0.412)and DCR(90.3%versus 88.1%,P=0.443)were also similar between the LP and GP group.A significantly lower proportion of patients in the LP group experienced adverse events(AEs)leading to treatment interruptions(10.9%versus 26.4%,P<0.001)or treatment termination(14.3%versus 23.1%,P=0.011).The analysis of cytokine levels in the LP group showed that low baseline levels of 27 cytokines were associated with an increased ORR,and 15 cytokines were associated with improved PFS,with 14 cytokines,including TNF-a,IFN-y,IL-6,and IL-8,demonstrating an overlapping trend.Conclusion:The LP regimen demonstrated similar PFS,OS,ORR and DCR as the GP regimen for patients with locally advanced or metastatic LSCC but had more favorable toxicity profiles.The study also identified a spectrum of different cytokines that could be potentially associated with the clinical benefit in patients who received the LP regimen. | Jie Zhang Yueyin Pan Qin Shi Guojun Zhang Liyan Jiang Xiaorong Dong Kangsheng Gu Huijuan Wang Xiaochun Zhang Nong Yang Yuping Li Jianping Xiong Tienan Yi Min Peng Yong Song Yun Fan Jiuwei Cui Gongyan Chen Wei Tan Aimin Zang Qisen Guo Guangqiang Zhao Ziping Wang Jianxing He Wenxiu Yao Xiaohong Wu Kai Chen Xiaohua Hu Chunhong Hu Lu Yue Da Jiang Guangfa Wang Junfeng Liu Guohua Yu Junling Li Jianling Bai Wenmin Xie Weihong Zhao Lihong Wu Caicun Zhou | 2022 | Cancer Communications2022，42，1： | 1 |
| 15 | Preparation and characterization of the graft copolywer of Chitosan with poly显示文摘 | Weigui Duan Chunhong Chen Linbin Jiang | 2008 | Carbohydrate Polyners2008，73，： | 1 |
| 16 | Extraction andpurification of wheat-esterase using aqueous two-phase systems ofionic liquid and salt显示文摘 | JIANG Bin FENG Zhibiao LIU Chunhong | 2015 | Journal of Food Science and Technology2015，52，5： | 1 |
| 17 | Characterization of a Hemolysin Gene ytjA from Bacillus subtilis显示文摘 | Jie Liu Chunhong Fang Yanlong Jiang Ruoyu Yan | 2009 | Current Microbiology2009，，6： | 1 |
| 18 | Preparation and characterization of the graft copolymer of ehitosan with poly显示文摘 | Duan Weigui Chen Chunhong Jiang Linbin | 2008 | Carbohydrate Polymers2008，73，4： | 1 |
| 19 | Molecular characterization and phylogenetic analysis of the reticuloendotheliosis virus isolated from wild birds in Northeast China显示文摘 | Lili Jiang Xiaole Qi Yulong Gao Yuping Hua Kai Li Xiaoyun Deng Qi Wang Lizhou Zhang Hongliang Chai Yuming Chen Chunhong Yin Honglei Gao Liting Qin Yongqiang Wang Yue Qu Qiang Chen Zhaobin Fan Xiaomei Wang | 2013 | Veterinary Microbiology (-)2013，，1： | 1 |
| 20 | ScientiaSinica Chimica显示文摘 | Sun Lixian Song Lifang Jiang Chunhong | 2010 | 40(9): 12432010，40，9： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员