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| 1 | Factors Affecting the Performance of Single-Chamber Soil Microbial Fuel Cells for Power Generation显示文摘There is limited information about the factors that affect the power generation of single-chamber microbial fuel cells(MFCs) using soil organic matter as a fuel source. We examined the effect of soil and water depths, and temperature on the performance of soil MFCs with anode being embedded in the flooded soil and cathode in the overlaying water. Results showed that the MFC with 5 cm deep soil and 3 cm overlaying water exhibited the highest open circuit voltage of 562 mV and a power density of 0.72 mW m-2. The ohmic resistance increased with more soil and water. The polarization resistance of cathode increased with more soil while that of anode increased with more water. During the 30 d operation, the cell voltage positively correlated with temperature and reached a maximum of 162 mV with a 500 ? external load. After the operation, the bacterial 16S rRNA gene from the soil and anode was sequenced. The bacteria in the soil were more diverse than those adhere to the anode where the bacteria were mainly affiliated to Escherichia coli and Deltaproteobacteria. In summary, the two bacterial groups may generate electricity and the electrical properties were affected by temperature and the depth of soil and water. | DENG Huan WU Yi-Cheng ZHANG Fan HUANG Zong-Chuan CHEN Zheng XU Hui-Juan ZHAO Feng | 2014 | Pedosphere2014，24，3： | 15 |
| 2 | Human placenta-derived mesenchymal stem cells loaded on linear ordered collagen scaffold improves functional recovery after completely transected spinal cord injury in canine显示文摘Traumatic spinal cord injury(SCI) is a major challenge in the clinic. In this study, we sought to examine the synergistic effects of linear ordered collagen scaffold(LOCS) and human placenta-derived mesenchymal stem cells(hPMSCs) when transplanted into completely transected beagle dogs. After 36 weeks observation, we found that LOCS+hPMSCs implants promoted better hindlimb locomotor recovery than was observed in the non-treatment(control) group and LOCS group. Histological analysis showed that the regenerated tissue after treatment was well integrated with the host tissue, and dramatically reduced the volume of cystic and chondroitin sulfate proteoglycans(CSPGs) expression. Furthermore, the LOCS+hPMSCs group also showed more neuron-specific βIII-tubulin(Tuj-1)-and NeuN-positive neurons in the lesion area, as well as axonal regeneration, remyelination and synapse formation in the lesion site. Additionally, dogs in the LOCS+hPMSCs group experienced enhanced sprouting of both ascending(CGRP-positive) sensory fibers and descending(5-HT-and TH-positive) motor fibers at the lesion area. All these data together suggested that the combined treatment had beneficial effects on neuronal regeneration and functional improvement in a canine complete transection model. Therefore, LOCS+hPMSCs implantation holds a great promise for bridging the nerve defect and may be clinically useful in the near future. | Sufang Han Zhifeng Xiao Xing Li Huan Zhao Bin Wang Zhixue Qiu Zhi Li Xin Mei Bai Xu Caixia Fan Bing Chen Jin Han Yanzheng Gu Huilin Yang Qin Shi Jianwu Dai | 2018 | Science China(Life Sciences)2018，61，1： | 15 |
| 3 | A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice显示文摘The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI).However,the calcium channels responsible for this process have remained unknown.Here,we show that rice CDSI(CELL DEATH and SUSCEPTIBLE to BLAST 1)encoding OsCNGC9;a cyclic nucleotide-gated channel protein,positively regulates the resistance to rice blast disease.We show that OsCNGC9 mediates PAMP-induced Ca2+influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression.We further show that a PTI related receptor-lih cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity.Our results suggest a signaling cascade linking pattern recognition to calcium channel activation,which is required for initiation of PTI and disease resistance in rice. | Jiachang Wang Xi Liu An Zhang Yulong Ren Fuqing Wu Gang Wang Yang Xu Cailin Lei Shanshan Zhu Tian Pan Yongfei Wang Huan Zhang Fan Wang Yan-Qiu Tan Yupeng Wang Xin Jin Sheng Luo Chunlei Zhou Xiao Zhang Jinling Liu Shuai Wang Lingzhi Meng Yihua Wang Xi Chen Qibing Lin Xin Zhang Xiuping Guo Zhijun Cheng Jiulin Wang Yunlu Tian Shijia Liu Ling Jiang Chuanyin Wu Ertao Wang Jian-Min Zhou Yong-Fei Wang Haiyang Wang Jianmin Wan | 2019 | Cell Research2019，29，10： | 13 |
| 4 | Estimating grassland LAI using the Random Forests approach and Landsat imagery in the meadow steppe of Hulunber, China显示文摘Leaf area index(LAI) is a key parameter for describing vegetation structures and is closely associated with vegetative photosynthesis and energy balance. The accurate retrieval of LAI is important when modeling biophysical processes of vegetation and the productivity of earth systems. The Random Forests(RF) method aggregates an ensemble of decision trees to improve the prediction accuracy and demonstrates a more robust capacity than other regression methods. This study evaluated the RF method for predicting grassland LAI using ground measurements and remote sensing data. Parameter optimization and variable reduction were conducted before model prediction. Two variable reduction methods were examined: the Variable Importance Value method and the principal component analysis(PCA) method. Finally,the sensitivity of RF to highly correlated variables was tested. The results showed that the RF parameters have a small effect on the performance of RF,and a satisfactory prediction was acquired with a root mean square error(RMSE) of 0.1956. The two variable reduction methods for RF prediction produced different results; variable reduction based on the Variable Importance Value method achieved nearly the same prediction accuracy with no reduced prediction,whereas variable reduction using the PCA method had an obviously degraded result that may have been caused by the loss of subtle variations and the fusion of noise information. After removing highly correlated variables,the relative variable importance remained steady,and the use of variables selected based on the best-performing vegetation indices performed better than the variables with all vegetation indices or those selected based on the most important one. The results in this study demonstrate the practical and powerful ability of the RF method in predicting grassland LAI,which can also be applied to the estimation of other vegetation traits as an alternative to conventional empirical regression models and the selection of relevant variables used in ecological models. | LI Zhen-wang XIN Xiao-ping TANG Huan YANG Fan CHEN Bao-rui ZHANG Bao-hui | 2017 | Journal of Integrative Agriculture2017，16，2： | 11 |
| 5 | TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus显示文摘Objective To detect Japanese encephalitis virus(JEV) rapidly and distinguish its genotypes, a TaqMan-based reverse transcriptase quantitative polymerase chain reaction(RT-PCR) detection system was developed.Methods By aligning the full-length sequences of JEV(G1-G5), six sets of highly specific TaqMan real-time RT-PCR primers and probes were designed based on the highly conserved NS1, NS2, and M genes of JEV, which included one set for non-specific JEV detection and five sets for the detection of specific JEV genotypes. Twenty batches of mosquito samples were used to evaluate our quantitative PCR assay.Results With the specific assay, no other flavivirus were detected. The lower limits of detection of the system were 1 pfu/mL for JEV titers and 100 RNA copies/μL. The coefficients of variation of this real-time RT-PCR were all < 2.8%. The amplification efficiency of this method was between 90% and 103%.Conclusion A TaqMan real-time RT-PCR detection system was successfully established to detect and differentiate all five JEV genotypes. | SHAO Nan LI Fan NIE Kai FU Shi Hong ZHANG Wei Jia HE Ying LEI Wen Wen WANG Qian Ying LIANG Guo Dong CAO Yu Xi WANG Huan Yu | 2018 | Biomedical and Environmental Sciences2018，31，3： | 10 |
| 6 | Detection of Pseudorabies Virus Antibodies in Human Encephalitis Cases显示文摘Pseudorabies virus(PRV),a veterinary pathogen that infects domestic animals as well as wild animals such as wild boar and feral swine,was recently reported to infect human and led to endophthalmitis and encephalitis.A retrospective seroepidemiologic survey was conducted using 1,335 serum samples collected from patients with encephalitis and ELISA positive rates were 12.16%,14.25%,and 6.52%in 2012,2013,and 2017,respectively. | LI Xiang Dong FU Shi Hong CHEN Ling Yan LI Fan DENG Jun Hua LU Xuan Cheng WANG Huan Yu TIAN Ke Gong | 2020 | Biomedical and Environmental Sciences2020，33，6： | 9 |
| 7 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
| 8 | Magnetic nanoparticles based cancer therapy: current status and applications显示文摘Nanotechnology holds a promising potential for developing biomedical nanoplatforms in cancer therapy. The magnetic nanoparticles, which integrate uniquely appealing features of magnetic manipulation, nanoscale heat generator, localized magnetic field and enzyme-mimics, prompt the development and application of magnetic nanoparticles-based cancer medicine. Considerable success has been achieved in improving the magnetic resonance imaging(MRI) sensitivity, and the therapeutic function of the magnetic nanoparticles should be given adequate attention. This work reviews the current status and applications of magnetic nanoparticles based cancer therapy. The advantages of magnetic nanoparticles that may contribute to improved therapeutics efficacy of clinic cancer treatment are highlighted here. | Huan Zhang Xiao Li Liu Yi Fan Zhang Fei Gao Ga Long Li Yuan He Ming Li Peng Hai Ming Fan | 2018 | Science China(Life Sciences)2018，61，4： | 8 |
| 9 | Endometrial MicroRNA Signature during the Window of Implantation Changed in Patients with Repeated Implantation Failure显示文摘 | Cheng Shi Huan Shen Li-Juan Fan Jing Guan Xin-Bang Zheng Xi Chen Rong Liang Xiao-Wei Zhang Qing-Hua Cui Kun-Kun Sun Zhu-Ran Zhao Hong-Jing Han | 2017 | Chinese Medical Journal2017，，5： | 8 |
| 10 | Risk of Active Pulmonary Tuberculosis among Patients with Coal Workers'Pneumoconiosis:A Case-control Study in China显示文摘The aim of this study was to estimate the association between occupational, environmental, behavioral risk factors, and active pulmonary tuberculosis(PTB) among coal workers' pneumoconiosis(CWP) patients. A matched case-control study was conducted in 86 CWP patients with active PTB and 86 CWP controls without TB. A standardized questionnaire was used for risk factors assessment. Conditioned logistic regression analysis was used to identify associations between the risk factors and active PTB among CWP patients. The results showed that the stage of CWP, poor workplace ventilation, family history of TB, and exposure to TB were independent risk factors for active PTB in patients with CWP with which recommendations for improving work environments, and for case finding activities in patients with CWP could be made. | JIN Yan FAN Jing Guang PANG Jing WEN Ke ZHANG Pei Ying WANG Huan Qiang LI Tao | 2018 | Biomedical and Environmental Sciences2018，31，6： | 8 |
| 11 | Epidemiologic characteristics of oral cancer:single-center analysis of 4097 patients from the Sun Yat-sen University Cancer Center显示文摘Background:Oral cancer is a common type of head and neck cancers.Knowing its epidemiologic characteristics is crucial to preventing,diagnosing,and treating this cancer.This study aimed to explore the epidemiologic characteristics of oral cancer in South China.Methods:We retrospectively analyzed data from 4097 oral cancer patients treated at the Sun Yat-sen University Cancer Center between 1960 and 2013.We compared the age of onset,sex ratio,pathologic type,and primary tumor location among three subcultural areas(Guangfu,Hakka,and Chaoshan) and between an economically developed region and a less-developed one in Guangdong.Results:Overall,oral cancer had a male-to-female ratio of approximately 2:1,and this ratio decreased over time.Oral cancer occurred mostly in patients of 45-64 years old(54.5%),and the percentage of older patients gradually increased over time.The most common tumor location was the tongue.Squamous cell carcinoma was the predominant pathologic type.The percentage of blood type O in oral cancer patients was lower than that in the healthy population.The male-to-female ratio in the Chaoshan area was higher than that in the Guangfu and Hakka areas,whereas the age of disease onset in Guangfu was higher than that in Hakka and Chaoshan.The male-to-female ratio was lower and the age of disease onset was higher in the economically developed region than in the less-developed region.Conclusion:The incidence of oral cancer in South China presents typical characteristics to which doctors should pay attention when diagnosing and treating oral cancer patients. | Ji Zhang Fan Gao An-Kui Yang Wen-Kuan Chen Shu-Wei Chen Huan Li Xing Zhang Zhong-Yuan Yang Xin-Lin Chen Ming Song | 2016 | Chinese Journal of Cancer2016，35，4： | 7 |
| 12 | The mutational pattern of homologous recombination(HR)-associated genes and its relevance to the immunotherapeutic response in gastric cancer显示文摘Objective:Currently,there is an urgent need to identify immunotherapeutic biomarkers to increase the benefit of immune checkpoint inhibitors(ICIs)for patients with gastric cancer(GC).Homologous recombination deficiency(HRD)can modify the tumor immune microenvironment by increasing the presence of tumor-infiltrating lymphocytes and therefore might serve as a biomarker of immunotherapeutic response.We aimed to analyze the mutational pattern of HR-associated genes in Chinese patients with GC and its relevance to the tumor immune profile and clinical immunotherapeutic response.Methods:A panel of 543 cancer-associated genes was used to analyze genomic profiles in a cohort comprising 484 Chinese patients with GC.Correlations between HR gene mutations and tumor immunity or clinical outcomes were identified via bioinformatic analysis using 2 GC genomic datasets(TCGA and MSK-IMPACT).Results:Fifty-one of the 484(10.54%)patients carried at least one somatic mutation in an HR gene;ATM(16/484,3.31%)was among the most frequently mutated HR genes in the Chinese cohort.Mutations in HR genes were associated with elevated tumor mutational burden,enhanced immune activity,and microsatellite instability status.In the MSK-IMPACT cohort comprising 49 patients with stomach adenocarcinoma or gastroesophageal junction adenocarcinoma treated with ICIs,patients with HR-mut GC(n=12)had significantly better overall survival than those with HR-wt GC(n=37)(log-rank test,P<0.05).Conclusions:Our data suggest that detection of somatic mutations in HR genes might aid in identifying patients who might benefit from immune checkpoint blockade therapy. | Yue Fan Haifeng Ying Xueying Wu Huan Chen Ying Hu Henghui Zhang Lijia Wu Ying Yang Beibei Mao Lan Zheng | 2020 | Cancer Biology & Medicine2020，17，4： | 6 |
| 13 | Effect of miniscalpel-needle on relieving the pain of myofascial pain syndrome: a systematic review显示文摘OBJECTIVE: To evaluate the effect and safety of miniscalpel-needle(MSN) on reducing the pain of myofascial pain syndrome(MPS).METHODS: We reviewed the available literatures inception up to February 2014 using Pubmed, EMBASE, Cochrane Library, Chinese National Knowledge Infrastructure Database, Chinese Biomedical Database and Wanfang Database.RESULTS: Eight randomized controlled trials were finally identified. The main controls involved acupuncture, medications, injection, massage and cupping. We found that all of the studies agreed on the potential benefit of MSN as a strategy for MPS and the superiority compared to the controls, however,randomized methods applied in most of the trials could be criticized for their high or unclear risk of bias. Further research is also needed to clarify questions around the appropriate frequency and number of treatment sessions of MSN.CONCLUSION: This review shows that MSN might have the effect on MPS, even though there were some limitations in the studies included in the review. Studies with robust methodology are warranted to further test its pain-relieving effect on MPS. | Liu Tong Peng Yuanyuan Zhu Shipeng Chen Huan Li Fuyun Hong Peixin Cao Bingyan Peng Bo Fan Yifan Chen Yupei Zhang Li | 2015 | Journal of Traditional Chinese Medicine2015，35，6： | 6 |
| 14 | Consuming fermented distillers' dried grains with solubles (DDGS) feed reveals a shift in the faecal microbiota of growing and fattening pigs using 454 pyrosequencing显示文摘The objective of this study was to investigate pig fed by Bacillus coagulans-fermented distillers' dried grains with solubles(DDGS)on the faecal microbial composition and diversity using 454 pyrosequencing. Healthy crossbred(Duroc×Yorkshire×Landrace)growing and fattening pigs(n=48),with an average initial body weight of 65 kg,were divided into two groups(24 replicates per group; four pens per group; six pigs per pen),and given either DDGS feed as the control,or B. coagulans-fermented DDGS feed as the treatment. Faecal samples were collected on day 0,7,14,21,and 28. DNA was extracted,and the V3–V6 region of the 16S rRNA gene was amplified. The fermented DDGS feed affected the relative abundance of bacteria populations at the phylum,genus,and species levels. At the genus level,the consumption of fermented DDGS feed led to higher relative abundances of faecal Prevotella,Lactobacillus,Clostridium,Bifidobacterium,Roseburia,and Bacillus,and lower relative abundances of faecal Escherichia,Ruminococcus,Dialister,unclassified Lachnospiraceae,unclassified Ruminococcaceae,and unclassified Enterobacteriaceae than in the control. At the species level,the consumption of fermented DDGS feed led to higher relative abundances of faecal Prevotella sp.,Lactobacillus johnsonii,Lactobacillus fermentum,Lactobacillus mucosae,Lactobacillus reuteri,Clostridium butyricum,Bifidobacterium sp.,and Roseburia sp.,and lower relative abundances of faecal Prevotella copri,Escherichia coli,Ruminococcus gnavus,Ruminococcus flavefaciens,and Dialister sp. than in the control. Principal coordinates analysis indicated a distinct separation in the faecal microbial communities of pigs that were fed the fermented and unfermented DDGS feed. Fermented DDGS feed significantly increased the average daily gain(ADG)of pigs,and significantly decreased the average daily feed intake(ADFI)of feed and feed/gain(F/G). Thus,our results demonstrate a beneficial shift in the faecal microbiota of pigs consuming fermented DDGS feed,with potential applications in livestock production. | WANG Jin HAN Ye ZHAO Jin-zhao ZHOU Zhi-jiang FAN Huan | 2017 | Journal of Integrative Agriculture2017，16，4： | 6 |
| 15 | Strength Prediction of Aluminum–Stainless Steel-Pulsed TIG Welding–Brazing Joints with RSM and ANN显示文摘Pulsed TIG welding–brazing process was applied to join aluminum with stainless steel dissimilar metals. Major parameters that affect the joint property significantly were identified as pulsed peak current, base current, pulse on time,and frequency by pre-experiments. A sample was established according to central composite design. Based on the sample,response surface methodology(RSM) and artificial neural networks(ANN) were employed to predict the tensile strength of the joints separately. With RSM, a significant and rational mathematical model was established to predict the joint strength.With ANN, a modified back-propagation algorithm consisting of one input layer with four neurons, one hidden layer with eight neurons, and one output layer with one neuron was trained for predicting the strength. Compared with RSM, average relative prediction error of ANN was \10% and it obtained more stable and precise results. | Huan He Chunli Yang Zhe Chen Sanbao Lin Chenglei Fan | 2014 | Acta Metallurgica Sinica(English Letters)2014，27，6： | 6 |
| 16 | 3D printing of calcium phosphate bioceramic with tailored biodegradation rate for skull bone tissue reconstruction显示文摘The bone regenerative scaffold with the tailored degradation rate matching with the growth rate of the new bone is essential for adolescent bone repair.To satisfy these requirement,we proposed bone tissue scaffolds with controlled degradation rate using osteoinductive materials(Ca-P bioceramics),which is expected to present a controllable biodegradation rate for patients who need bone regeneration.Physicochemical properties,porosity,compressive strength and degradation properties of the scaffolds were studied.3D printed Ca-P scaffold(3DS),gas foaming Ca-P scaffold(FS)and autogenous bone(AB)were used in vivo for personalized beagle skull defect repair.Histological results indicated that the 3DS was highly vascularized and well combined with surrounding tissues.FS showed obvious newly formed bone tissues.AB showed the best repair effect,but it was found that AB scaffolds were partially absorbed and degraded.This study indicated that the 3D printed Ca-P bioceramics with tailored biodegradation rate is a promising candidate for personalized skull bone tissue reconstruction. | Boqing Zhang Huan Sun Lina Wu Liang Ma Fei Xing Qingquan Kong Yujiang Fan Changchun Zhou Xingdong Zhang | 2019 | Bio-Design and Manufacturing2019，2，3： | 5 |
| 17 | Geochemical baseline determination and contamination of heavy metals in the urban topsoil of Fuxin City,China显示文摘Urban topsoil is the most frequent interface between human society and natural environment.The accumulation of heavy metals in the urban topsoil has a direct effect on residents'life and health.The geochemical baseline of heavy metals is an objective description of the general level of heavy metals in the urban topsoil.Meanwhile,the determination of geochemical baseline is necessary for regional environmental management,especially in coal cities prone to heavy metal pollution.Heavy metal pollution has become an environmental problem in Fuxin City,China for a long time.To establish the geochemical baseline of heavy metals in the topsoil of Fuxin City and to evaluate the ecological risk of the topsoil,we collected 75 topsoil samples(0–20 cm)and analyzed the concentrations of Cu,Ni,Zn,Pb,Cr,Cd,Hg and As through X-ray fluorescence spectrometry,atomic absorption spectrometry and inductively coupled plasma optical emission spectrometry.We determined the geochemical baseline of heavy metals in the topsoil of Fuxin City by using iteration removal,box-whisker plot,cumulative frequency curve and reference metal normalization;evaluated the contamination risk and ecological risk of the topsoil by using the baseline factor index,Nemerow index and Hakanson potential ecological risk index;and identified the source category of heavy metals in the topsoil by using a pedigree clustering heatmap.Results showed that the geochemical baseline values were 42.86,89.34,92.23,60.55,145.21,0.09,0.08 and 4.17 mg/kg for Cu,Ni,Zn,Pb,Cr,Cd,Hg and As,respectively.The results of Nemerow index and Hakanson potential ecological risk index indicated that the urban topsoil in the study area was slightly contaminated and suffering low potential ecological risk.The main contaminated areas dominated in the middle part and northeast part of the study area,especially in the western Haizhou Strip Mine.The result of baseline factor index indicated that Hg and Cd were the major pollution elements.Using a pedigree clustering heatmap,we divided the sources of these heavy metals into three types:type I for Ni and Cr,largely represented the enrichment of heavy metals from natural sources;type II for Cu,Pb,Zn,Cd and As,mainly represented the enrichment of heavy metals from anthropogenic sources;and type III for Hg,represented the form of both natural and anthropogenic inputs. | ZHANG Hua YU Miao XU Hongjia WEN Huan FAN Haiyan WANG Tianyi LIU Jiangang | 2020 | Journal of Arid Land2020，12，6： | 5 |
| 18 | Structural Engineering of Hierarchical Aerogels Comprised of Multi-dimensional Gradient Carbon Nanoarchitectures for Highly Efficient Microwave Absorption显示文摘Recently,multilevel structural carbon aerogels are deemed as attractive candidates for microwave absorbing materials.Nevertheless,excessive stack and agglomeration for low-dimension carbon nanomaterials inducing impedance mismatch are significant challenges.Herein,the delicate“3D helix-2D sheet-1D fiber-0D dot”hierarchical aerogels have been successfully synthesized,for the first time,by sequential processes of hydrothermal self-assembly and in-situ chemical vapor deposition method.Particularly,the graphene sheets are uniformly intercalated by 3D helical carbon nanocoils,which give a feasible solution to the mentioned problem and endows the as-obtained aerogel with abundant porous structures and better dielectric properties.Moreover,by adjusting the content of 0D core-shell structured particles and the parameters for growth of the 1D carbon nanofibers,tunable electromagnetic properties and excellent impedance matching are achieved,which plays a vital role in the microwave absorption performance.As expected,the optimized aerogels harvest excellent performance,including broad effective bandwidth and strong reflection loss at low filling ratio and thin thickness.This work gives valuable guidance and inspiration for the design of hierarchical materials comprised of dimensional gradient structures,which holds great application potential for electromagnetic wave attenuation. | Yongpeng Zhao Xueqing Zuo Yuan Guo Hui Huang Hao Zhang Ting Wang Ningxuan Wen Huan Chen Tianze Cong Javid Muhammad Xuan Yang Xinnan Wang Zeng Fan Lujun Pan | 2021 | Nano-Micro Letters2021，13，9： | 5 |
| 19 | Modeling the Potential Geographic Distribution of Black Pepper(Piper nigrum)in Asia Using GIS Tools显示文摘Known as the 'king of spices',black pepper(Piper nigrum),a perennial crop of the tropics,is economically the most important and the most widely used spice crop in the world.To understand its suitable bioclimatic distribution,maximum entropy based on ecological niche modeling was used to model the bioclimatic niches of the species in its Asian range.Based on known occurrences,bioclimatic areas with higher probabilities are mainly located in the eastern and western coasts of the Indian Peninsula,the east of Sumatra Island,some areas in the Malay Archipelago,and the southeast coastal areas of China.Some undocumented places were also predicted as suitable areas.According to the jackknife procedure,the minimum temperature of the coldest month,the mean monthly temperature range,and the precipitation of the wettest month were identified as highly effective factors in the distribution of black pepper and could possibly account for the crop's distribution pattern.Such climatic requirements inhibited this species from dispersing and gaining a larger geographical range. | HAO Chao-yun FAN Rui Milton Cezar Ribeiro TAN Le-he WU Hua-song YANG Jian-feng ZHENG Wei-quan YU Huan | 2012 | Journal of Integrative Agriculture2012，11，4： | 4 |
| 20 | A Reverse-transcription Recombinase-aided Amplification Assay for the Rapid Detection of the Far-Eastern Subtype of Tick-borne Encephalitis Virus显示文摘Objective Tick-borne encephalitis virus(TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis(TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease. Methods A reverse-transcription recombinase-aided amplification(RT-RAA) assay was developed. This assay can be completed in one closed tube at 39℃ within 30 minutes. The sensitivity and specificity of RT-RAA were validated using non-infectious synthetic RNA representing a fragment of the NS5 region of the wild-type(WT) TBEV genome and the Senzhang strain. Additionally, 10 batches of tick samples were used to evaluate the performance of the RT-RAA assay. Results The analytical limit of detection of the assay was 20 copies per reaction of the TBEV synthetic transcript and 3 plaque-forming units(pfu) per reaction of TBEV titers. With the specific assay, no signal due to other arboviruses was observed. Of the 10 batches of tick samples obtained from the Changbai Mountains of China, three were TBEV-positive, which was consistent with the results of the quantitative real-time PCR assay. Conclusion A rapid, highly sensitive, specific, and easy-to-use method was developed for the detection of the TBEV Far-Eastern subtype. | WANG Qian Ying LI Fan SHEN Xin Xin FU Shi Hong HE Ying LEI Wen Wen LIANG Guo Dong WANG Huan Yun MA Xue Jun | 2019 | Biomedical and Environmental Sciences2019，32，5： | 4 |
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