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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Chinese Multidisciplinary Expert Consensus on the Diagnosis and Treatment of Hyperuricemia and Related Diseases显示文摘 | Zou, He-Jian Wu, Hu-Sheng Zhou, Jing-Guo Zeng, Xue-Jun Dai, Lie Wu, Hua-Xiang Zhu, Xiao-Xia Mei, Chang-Lin Hao, Chuan-Ming Chen, Nan Liu, Bi-Cheng Chen, Jiang-Hua Yang, Li Nie, Jing Yu, Chen Peng, Ai Yu, Sheng-Qiang Li, Lin Ge, Jun-Bo Huo, Yong Zhang, Shu-Yang Chen, Yun-Dai Dong, Yu-Gang Liang, Chun Dai, Yu-Xiang Gao, Xin Li, Chang-Gui Zhao, Jia-Jun Chen, Hai-Bing Cheng, Zhi-Feng Lin, Huan-Dong Guan, Yang-Tai Wang, Kai Luo, Ben-Yan Dai, Ruo-Lian Jiang, Quan Xue, Luan Liang, Chao-Chao Chen, Ming Fan, Song | 2017 | Chinese Medical Journal2017，，20： | 30 |
| 3 | Glacial change in the vicinity of Mt. Qomolangma (Everest), central high Himalayas since 1976显示文摘冰河是在高山的区域的最重要的陆地封面之一并且对全球气候特别敏感变化。遥感证明了是在遥远的多山的区域调查冰川的变化的程度的最好的方法。用 Landsat 题目的印射(TM ) 并且从科莫朗马山(珠穆琅玛) 的 multi-spectral-scanner (女士) 图象国家自然保存(QNNP ) ,为 1976, 1988 和 2006 的中央高喜马拉雅山脉,我们为这三个时期导出冰川的程度。面向对象的图象解释方法,专家知识规则和域调查的联合被采用。结果证明(1 ) 在 2006 的冰川的区域是 2710.17 卤 0.011 km2 (大约 7.41% 整个学习区域) ,并且主要定位了到南方并且在 4700 m 之间到上面的 6800 m 海水平;(2 ) 从 1976 ~ 2006, 0.035 km2 和冰川的湖由 36.88 卤扩展了的 501.91 卤减少的冰河 0.035 km2;冰河撤退的率比在北斜坡(14.40%) 上在喜马拉雅山脉的南部的斜坡(16.79%) 上的亚盆是更高的;大多数冰河在 4700 鈥 ? 的举起撤退了,并且主要发生了 400 m,和撤退地区的估计的上面的限制在 6600 m 和 6700 m 之间;(3 ) 温度的增加和在在学习时期上的降水的减少是驾驶撤退的关键因素。 | NIE Yong ZHANG Yili LIU Linshan ZHANG Jiping | 2010 | Journal of Geographical Sciences2010，20，5： | 22 |
| 4 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 5 | CYP2C19*2 and Other Allelic Variants Affecting Platelet Response to Clopidogrel Tested by Thrombelastography in Patients with Acute Coronary Syndrome显示文摘 | Jian Liu Xiao-Yan Nie Yong Zhang Yun Lu Lu-Wen Shi Wei-Min Wang | 2015 | Chinese Medical Journal2015，，16： | 19 |
| 6 | Predicting Potential Distribution of Tibetan Spruce (Picea smithiana) in Qomolangma (Mount Everest) National Nature Preserve Using Maximum Entropy Niche-based Model显示文摘Tibetan spruce (Picea smithiana) is an endemic species of the Himalayas,and it distributes only in a re-stricted area with very low number.To address the lack of detailed distributional information,we used maximum en-tropy (Maxent) niche-based model to predict the species' potential distribution from limited occurrence-only records.The location data of P.smithiana,relative bioclimatic variables,vegetation data,digital elevation model (DEM),and the derived data were analyzed in Maxent.The receiver operating characteristic (ROC) curve was applied to assess the prediction accuracy.The Maxent jackknife test was performed to quantify the training gains from data layers and the response of P.smithiana distribution to four typical environmental variables was analyzed.Results show that the model performs well at the regional scale.There is a potential for continued expansion of P.smithiana population numbers and distribution in China.P.smithiana potentially distributes in the lower reaches of Gyirong Zangbo and Poiqu rivers in Gyirong and Nyalam counties in Qomolangma (Mount Everest) National Nature Preserve (QNNP),China.The species prefers warm temperate climate in mountain area and mainly distributes in needle-leaved evergreen closed to open forest and mixed forest along the river valley at relatively low altitudes of about 2000-3000 m.Model simulations suggest that distribution patterns of rare species with few species numbers can be well predicted by Max-ent. | ZHANG Jiping ZHANG Yili LIU Linshan NIE Yong | 2011 | Chinese Geographical Science2011，21，4： | 15 |
| 7 | Nondestructive determination of nutritional information in oilseed rape leaves using visible/near infrared spectroscopy and multivariate calibrations显示文摘Nitrogen, phosphorus and potassium content are the three most important nutritional parameters for growing oilseed rape. We investigated visible and near infrared (Vis/NIR) spectroscopy combined with chemometrics for the fast and nondestructive determination of nutritional information in oilseed rape leaves. A total of 154 leaf samples were collected, with 104 randomly selected as the calibration set, and the remaining 50 samples used as the validation set. The performance of eight different preprocessing methods was compared in partial least squares (PLS) models. Some effective wavelengths selected by a successive projections algorithm (SPA) were also used to develop linear SPA-PLS, nonlinear back propagation neural network (BPNN), and nonlinear least squares-support vector machine (LS-SVM) models to determine nutritional information. The best prediction models were DOSC-PLS for nitrogen with r=0.9743 and RMSEP=0.1459, DOSC-SPA-BPNN for phosphorus with r=0.7054 and RMSEP=0.0594, and DOSC-SPA-BPNN for potassium with r=0.9380 and RMSEP=0.1788. The prediction precision for nitrogen and potassium determinations was acceptable for further practical applications, whereas further studies are needed to improve the prediction precision for phosphorus. The results indicated that Vis/NIR spectroscopy is feasible for nondestructive determination of nutritional information in oilseed rape leaves. It also provided an alternative technique for detecting other growth information about oilseed rape leaves. | LIU Fei NIE PengCheng HUANG Min KONG WenWen HE Yong | 2011 | Science China(Information Sciences)2011，54，3： | 14 |
| 8 | Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer显示文摘Cloning of macaque monkeys by somatic cell nucleus transfer(SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases. Here, we report the feasibility of this approach by SCNT of fibroblasts from a macaque monkey(Macaca fascicularis), in which a core circadian transcription factor BMAL1 was knocked out by clustered regularly interspaced short palindromic repeat/Cas9 gene editing(see accompanying paper).Out of 325 SCNT embryos transferred into 65 surrogate monkeys, we cloned five macaque monkeys with BMAL1 mutations in both alleles without mosaicism, with nuclear genes identical to that of the fibroblast donor monkey. Further peripheral blood m RNA analysis confirmed the complete absence of the wild-type BMAL1 transcript. This study demonstrates that the SCNT approach could be used to generate cloned monkeys from fibroblasts of a young adult monkeys and paves the way for the development of macaque monkey disease models with uniform genetic backgrounds. | Zhen Liu Yijun Cai Zhaodi Liao Yuting Xu Yan Wang Zhanyang Wang Xiaoyu Jiang Yuzhuo Li Yong Lu Yanhong Nie Xiaotong Zhang Chunyang Li Xinyan Bian Mu-ming Poo Hung-Chun Chang Qiang Sun | 2019 | National Science Review2019，6，1： | 14 |
| 9 | CPSF30-L-mediated recognition of mRNA m^(6)A modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis显示文摘N6-methyladenosine(m^(6)A),a ubiquitous internal modification of eukaryotic mRNAs,plays a vital role in almost every aspect of mRNA metabolism.However,there is little evidence documenting the role of m^(6)A in regulating alternative polyadenylation(APA)in plants.APA is controlled by a large protein-RNA complex with many components,including CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30(CPSF30).In Arabidopsis,CPSF30 has two isoforms and the longer isoform(CPSF30-L)contains a YT512-B Homology(YTH)domain,which is unique to plants.In this study,we showed that CPSF30-L YTH domain binds to m^(6)A in v itro.In the cpsf30-2 mutant,the transcripts of many genes including several important nitrate signaling-related genes had shifts in polyadenylation sites that were correlated with m^(6)A peaks,indicating that these gene transcripts carrying m^(6)A tend to be regulated by APA.Wild-type CPSF30-L could rescue the defects in APA and nitrate metabolism in cpsf30-2,but m^(6)A-binding-defective mutants of CPSF30-L could not.Taken together,our results demonstrated that m^(6)A modification regulates APA in Arabidops is and revealed that the m^(6)A reader CPSF30-L affects nitrate signaling by controlling APA,shedding new light on the roles of the m^(6)A modification during RNA 3-end processing in nitrate metabolism. | Yiteng Hou Jing Sun Baixing Wu Yangyang Gao Hongbo Nie Zhentian Nie Shuxuan Quan Yong Wang Xiaofeng Cao Sisi Li | 2021 | Molecular Plant2021，14，4： | 12 |
| 10 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 11 | Active injection protection scheme for flexible HVDC grids based on amplitude of input impedance显示文摘High-voltage direct current(HVDC)grids require fast and reliable protection of the DC lines.The performance of traditional protection schemes is easily impaired by the limitations of the boundary condition and nonlinearity from the control of converters.One of the key technologies for flexible HVDC grids is the half-bridge modular multilevel converter(HB-MMC).Considering the high controllability of HB-MMC,this study proposes an active injection protection scheme to improve the reliability and sensitivity of the HVDC grid protection.The HB-MMC is used to inject a sinusoidal characteristic signal,at the specified frequency,into the DC lines.Then,the voltage and current at the specified frequency are extracted using the Prony algorithm to calculate the input impedance,which is used for the identification of internal and external faults.The active injection protection scheme was simulated for various cases in the simulation software Power Systems Computer Aided Design.The simulation results indicate that the proposed protection scheme is highly reliable and can overcome transition resistance. | Jinghan He Keao Chen Meng Li Yong Tang Ming Nie | 2021 | Global Energy Interconnection2021，4，6： | 11 |
| 12 | Deep learning applied to two-dimensional color Doppler flow imaging ultrasound images significantly improves diagnostic performance in the classification of breast masses:a multicenter study显示文摘Background:The current deep learning diagnosis of breast masses is mainly reflected by the diagnosis of benign and malignant lesions.In China,breast masses are divided into four categories according to the treatment method:inflammatory masses,adenosis,benign tumors,and malignant tumors.These categorizations are important for guiding clinical treatment.In this study,we aimed to develop a convolutional neural network(CNN)for classification of these four breast mass types using ultrasound(US)images.Methods:Taking breast biopsy or pathological examinations as the reference standard,CNNs were used to establish models for the four-way classification of 3623 breast cancer patients from 13 centers.The patients were randomly divided into training and test groups(n=1810 vs.n=1813).Separate models were created for two-dimensional(2D)images only,2D and color Doppler flow imaging(2D-CDFI),and 2D-CDFI and pulsed wave Doppler(2D-CDFI-PW)images.The performance of these three models was compared using sensitivity,specificity,area under receiver operating characteristic curve(AUC),positive(PPV)and negative predictive values(NPV),positive(LR+)and negative likelihood ratios(LR-),and the performance of the 2D model was further compared between masses of different sizes with above statistical indicators,between images from different hospitals with AUC,and with the performance of 37 radiologists.Results:The accuracies of the 2D,2D-CDFI,and 2D-CDFI-PW models on the test set were 87.9%,89.2%,and 88.7%,respectively.The AUCs for classification of benign tumors,malignant tumors,inflammatory masses,and adenosis were 0.90,0.91,0.90,and 0.89,respectively(95%confidence intervals[CIs],0.87-0.91,0.89-0.92,0.87-0.91,and 0.86-0.90).The 2D-CDFI model showed better accuracy(89.2%)on the test set than the 2D(87.9%)and 2D-CDFI-PW(88.7%)models.The 2D model showed accuracy of 81.7%on breast masses≤1 cm and 82.3%on breast masses>1 cm;there was a significant difference between the two groups(P<0.001).The accuracy of the CNN classifications for the test set(89.2%)was significantly higher than that of all the radiologists(30%).Conclusions:The CNN may have high accuracy for classification of US images of breast masses and perform significantly better than human radiologists.Trial registration:Chictr.org,ChiCTR1900021375;http://www.chictr.org.cn/showproj.aspx?proj=33139. | Teng-Fei Yu Wen He Cong-Gui Gan Ming-Chang Zhao Qiang Zhu Wei Zhang Hui Wang Yu-Kun Luo Fang Nie Li-Jun Yuan Yong Wang Yan-Li Guo Jian-Jun Yuan Li-Tao Ruan Yi-Cheng Wang Rui-Fang Zhang Hong-Xia Zhang Bin Ning Hai-Man Song Shuai Zheng Yi Li Yang Guang | 2021 | Chinese Medical Journal2021，，4： | 11 |
| 13 | Risk factors of intra-abdominal bacterial infection after liver transplantation in patients with hepatocellular carcinoma显示文摘Objective: To explore the risk factors of intra-abdominal bacterial infection(IAI) after liver transplantation(LT) in patients with hepatocellular carcinoma(HCC). Methods: A series of 82 HCC patients who received LT surgeries in our department between March 2004 and April 2010 was recruited in this study. Then we collected and analyzed the clinical data retrospectively. Statistical analysis system(SPSS) software was adopted to perform statistical analysis. Chi-square test, t-test and Wilcoxon rank sum test were used to analyze the clinical data and compute the significance of the incidences of early-stage IAI after LT for HCC patients. Binary logistic regression was performed to screen out the risk factors, and multiple logistic regression analyses were performed to compute the independent risk factors. Results: A series of 13 patients(13/82, 15.9%) had postoperative IAI. The independent risk factors of postoperative intra-abdominal bacterial infections after LT for HCC patients were preoperative anemia [Hemoglobin(HGB) <90 g/L] and postoperative abdominal hemorrhage(72 hours >400 mL), with the odds ratios at 8.121(95% CI, 1.417 to 46.550, P=0.019) and 5.911(95% CI, 1.112 to 31.432, P=0.037). Conclusions: Postoperative IAI after LT in patients with HCC was a common complication. Preoperative moderate to severe anemia, as well as postoperative intra-abdominal hemorrhage more than 400 mL within the first 72 hours might independently indicate high risk of IAI for these patients. | Kai Nie Rongzheng Ran Weifeng Tan Bin Yi Xiangji Luo Yong Yu Xiaoqing Jiang | 2014 | Chinese Journal of Cancer Research2014，26，3： | 10 |
| 14 | Synthesis and fluorescent properties in complexes of Eu( Ⅲ), Tb( Ⅲ), and Sm( Ⅲ) with β-diketone and 2,2′-bipyridine显示文摘Three new rare earth ternary complexes, RE(PB)_3bpy (RE=Sm^(3+) , Eu^(3+) , and Tb^(3+) ), were synthesized by the reaction of 1-(p-phenylethynyl- phenyl)-1,3-butanedione (HPB) and 2,2′-bipyridine (bpy) with rare earth chloride RECl_3 , respectively, in alcohol solution. The compositions were characterized by means of infrared (IR) spectra, chemical analysis, elemental analysis, and thermodynamic analysis. Luminescent properties of the three complexes were studied. At room temperature, under UV light excitation, the Sm^(3+) , Eu^(3+) , and Tb^(3+) complexes exhibit characteristic emission of the central ions. The fluorescence spectra show that the fluorescence emission intensity of Eu^(3+) complex is the strongest. The narrow strongest emission band of Eu^(3+) complex is considered to be a valuable material with bright red fluorescence. | LIU Xingwang JIANG Jiadong GUO Yuwei YONG Shengli YANG Kun NIE Lu | 2012 | Rare Metals2012，31，5： | 9 |
| 15 | Comparison of basic features and origins of oolitic shoal reservoirs between carbonate platform interior and platform margin locations in the Lower Triassic Feixianguan Formation of the Sichuan Basin,southwest China显示文摘The oolitic shoal reservoirs of the Lower Triassic Feixianguan Formation carbonates in the Sichuan Basin of southwest China are an important target for gas exploration in the basin.Their occurrence,like other cases worldwide,can be divided into two locations in general,i.e.,platform interior and platform margin locations.Their differences of reservoir features and origins,however,have not been investigated comprehensively due to different exploration degrees.This issue is addressed in this paper,to provide basic data and information for the basin's hydrocarbon exploration and for the study of carbonate platform sedimentology and reservoir geology worldwide.We compared the features of these two types of reservoirs in detail,including the depositional and diagenetic features,pore types and petrophysical features.Based on the comparison,the origin of the reservoirs was further discussed.It is shown that the reservoirs in platform interior and platform margin locations differ significantly.The interior carbonates were deposited in moderate to high energy settings and the dominant lithologic type was limestone,which was weakly compacted and intensely cemented and has undergone meteoric dissolution.Pore types include intragranular dissolution and moldic pores,with low porosities(<6%) and low permeabilities(<0.1 mD).By contrast,the platform margin carbonates were deposited in relatively high energy settings and mainly consisted of dolostones with some limestones.The rocks were strongly compacted but incompletely cemented.As a result,some primary intergranular pores were preserved.Both meteoric solution and burial solution have taken place.There are various types of pore spaces including intergranular and intercrystalline solution pores and residual intergranular pores.This type of reservoir generally has better petrophysical properties(>9% porosity and >0.1 mD permeability) and pore-throat structures than the interior reservoirs.These differences were influenced by both primary depositional features and secondary diagenesis.For the interior carbonate reservoirs,early meteoric dissolution,weak compaction and strong cementation are important controlling factors.By contrast,the factors controlling the formation of the margin carbonate reservoirs mainly include dolomitization,preservation of primary pores and burial dissolution. | Tan Xiucheng Zhao Luzi Luo Bing Jiang Xingfu Cao Jian Liu Hong Li Ling Wu Xingbo Nie Yong | 2012 | Petroleum Science2012，9，4： | 9 |
| 16 | Fluidized mining and in-situ transformation of deep underground coal resources: a novel approach to ensuring safe, environmentally friendly, low-carbon, and clean utilisation显示文摘Traditional coal mining and utilisation patterns are severely detrimental to natural resources and environments and significantly impede safe, low-carbon, clean, and sustainable utilisation of coal resources. Based on the idea of in situ fluidized coal mining that aims to transform solid coal into liquid or gas and transports the fluidized resources to the ground to ensure safe mining and low-carbon and clean utilisation, in this study, we report on a novel in situ unmanned automatic mining method. This includes a flexible, earthworm-like unmanned automatic mining machine (UAMM) and a coal mine layout for in situ fluidized coal mining suitable for the UAMM. The technological and economic advantages and the carbon emission reduction of the UAMM-based in situ fluidized mining in contrast to traditional mining technologies are evaluated as well. The development trends and possible challenges to this design are also discussed. It is estimated that the proposed method costs approximately 49% of traditional coal mining costs. The UAMM-based in situ fluidized mining and transformation method will reduce CO2 emissions by at least 94.9% compared to traditional coal mining and utilisation methods. The proposed approach is expected to achieve safe and environmentally friendly coal mining as well as lowcarbon and clean utilisation of coal. | Yang Ju Yan Zhu Heping Xie Xiaodong Nie Yong Zhang Chang Lu Feng Gao | 2019 | International Journal of Coal Science & Technology2019，6，2： | 8 |
| 17 | Intermetallic PtBi core/ultrathin Pt shell nanoplates for efficient and I stable methanol and ethanol electro-oxidization显示文摘The development of Pt-based core/shell nanoparticles represents an emerging class of electrocatalysts for fuel cells, such as methanol oxidation reaction (MOR) and ethanol oxidation reaction (EOR). Here, we present a one-pot synthesis approach to prepare hexagonal PtBi/Pt core/shell nan ostructure composed of an in termetallic PtiBii core and an ultrathi n Pt shell with well-defined shape, size, and composition.The structure and the synergistic effect among different components enhanced their MOR and EOR performanee. The optimized Pt2Bi nanoplates exhibit excellent mass activities in both MOR (4,820 mA·mgpt^-1) and EOR (5,950 mA·mgpt^1) conducted in alkaline media, which are 6.15 times and 8.63 times higher than those of commercial Pt/C, respectively. Pt2Bi nan opiates also show superior operati on durability to commercial Pt/C. This work may inspire the rational design and synthesis of Pt-based nanoparticles with improved performance for fuel cells and other applications. | Xiaolei Yuan Xiaojing Jiang Muhan Cao Lei Chen Kaiqi Nie Yong Zhang Yong Xu Xuhui Sun Yanguang Li Qiao Zhang | 2019 | Nano Research2019，12，2： | 7 |
| 18 | Acute pulmonary inflammation is inhibited in CXCR3 knockout mice after short-term cigarette smoke exposure显示文摘瞄准:CXCR3,经由绑定它的特定的 ligand CXCL10,在导致的香烟烟(CS ) 起一个重要作用肺的发炎。CXCR3 优先地在激活的 T 房间被表示(主要 CD8 + T 房间) 。这研究的目的是用 CXCR3 在导致 CS 的肺的损害调查 CXCR3 的角色基因缺乏的(CXCR3−/−) 老鼠。方法:在煽动性的房间和 CD8 + T 房间和在在 mRNA 和蛋白质层次的 bronchoalveolar 洗室年龄液体和肺的煽动性的调停人和 chemokines 的表示的渗入的差别在 CXCR3−/− 之间被比较老鼠并且野类型(WT ) 在在 CS 暴露的 3 d 以后的 2 h 的老鼠。结果:与他们的 WT 对应物相比, CXCR3−/−老鼠显示出减轻的发炎,由更少煽动性的房间证实了特别地细胞毒素的 CD8 + T 房间,在 bronchoalveolar 洗室年龄液体和肺纸巾。在 mRNA 和蛋白质层次,有显著地在 CXCR3−/− 的煽动性、趋化性的 cytokines,包括的 TNF-α , interleukin-8, interferon-γ ,转变生长 factor-β1,和 CXCL10 的底层老鼠。结论:我们 CXCR3 在招募是重要的数据表演煽动性的房间(特别地 CD8 + T 房间) 进航线和肺,以及开始煽动性并且在跟随短期的 CS 侮辱的 2 h 的 fibrotic cytokines 版本。CXCR3 能是为 CS 导致的肺的发炎的处理的一个新奇目标。 | Li NIE Ruo-lan XIANG Yong LIU Wei-xun ZHOU Lei JIANG Bao LU Bao-sen PANG De-yun CHENG Jin-ming GAO | 2008 | Acta Pharmacologica Sinica2008，29，12： | 6 |
| 19 | Development of a multiplex reverse transcription-PCR assay for simultaneous detection of garlic viruses显示文摘A preliminary screening for garlic viruses in garlic plants in Hunan,China,using existing monoplex(simplex) reverse transcription-polymerase chain reaction(RT-PCR) procedures detected four viruses/virus groups.These viruses/virus groups were Onion yellow dwarf virus(OYDV),Leek yellow stripe virus(LYSV),Shallot latent virus(SLV) and allexiviruses(e.g.,garlic viruses A,B,C,D,E,X).Sequence analysis of the projected allexivirus amplicons revealed the allexivirus in the infected garlic plants was Garlic virus D(GarV-D),which shared 92-97%sequence identities with various isolates from the world.A multiplex RT-PCR(mRT-PCR) was therefore developed to simultaneously detect and differentiate the four viruses/virus groups.To achieve this,four primer pairs targeting allexiviruses,OYDV,LYSV and SLV were designed.The anticipated amplicon sizes are 183 bp(allexiviruses),265 bp(OYDV),404 bp(LYSV) and 592 bp(SLV),respectively.All primer pairs produced virus-specific fragments in both simplex and multiplex formats,thus confirming the efficacy of the newly developed mRT-PCR for detection of these viruses.The mRT-PCR further was evaluated by applying it to garlic plant samples collected in two geographic locations in Hunan.Allexiviruses,OYDV,LYSV and SLV were detected in 50.9,40.3,28.3 and 58.5%of leaf samples,respectively;and mixed infections with two or more viruses accounted for 54%of the garlic samples.The results obtained by mRT-PCR were confirmed by simplex RT-PCR assays.In conclusion,this newly developed mRT-PCR provides a rapid,sensitive and reliable method for the detection and identification of major garlic viruses. | HU Xin-xi LEI Yan WANG Pei TANG Lin-fei HE Chang-zheng SONG Yong XIONG Xing-yao NIE Xian-zhou | 2015 | Journal of Integrative Agriculture2015，14，5： | 6 |
| 20 | Multi-dimension apportionment of clean air “parade blue”phenomenon in Beijing显示文摘The mass concentration and major chemical components of fine particulate matter were measured before,during and after Beijing's massive parade commemorating 70th anniversary of the Chinese Victory in World War II on September 3,2015.Regional emission inventory,positive matrix factorization(PMF),observations from space and backward air mass trajectories were jointly applied to identify the major pollution sources and their temporal and spatial variations.The contributions of emissions variations and the meteorological conditions related to the'parade blue'phenomenon in Beijing and its surrounding areas were investigated in detail.The main cause of the decreased PM_(2.5)mass concentration was attributed to the absolute reduction in emissions of primary air pollutants.The chemical composition of PM_(2.5)varied significantly before,during and after the parade.Fugitive dust particles were well controlled,the secondary formation of PM_(2.5)was reduced along with the controlled gaseous precursors'emissions from vehicles and industrial sources during the temporary intensified control period.During the parade period,the SO_2and NO_2column concentrations in Beijing and the surrounding areas decreased sharply,indicating that the coordinated reduction in primary emissions from the surrounding areas of Beijing played an important role in lowering the ambient concentration of SO_2and NO_2and accordingly lowered PM_(2.5)and improved the regional air quality.A comparison of the temperature,humidity,and wind speed and direction during the same periods in 2014 and 2015 showed that the meteorological conditions positively influenced the achievement of'parade blue'. | Yifeng Xue Yong Wang Xuefeng Li Hezhong Tian Lei Nie Xiaoqing Wu Junrui Zhou Zhen Zhou | 2018 | Journal of Environmental Sciences2018，30，3： | 5 |
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