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| 1 | TRICHOMEAND ARTEMISININ REGULATOR 1 Is Required for Trichome Development and Artemisinin Biosynthesis in Artemisia annua显示文摘毛状体,许多植物种类的表面上的小伸出,能生产并且存储各种各样的第二等的新陈代谢的产品。Artemisinin,最著名并且为疟疾的有势力药,被综合,存储,并且由 Artemisia annua 毛状体藏匿了。然而,糟糕在 A.annua 遗体调整 artemisinin 的生合成和毛状体的发展的分子的基础理解。这里,我们报导那一个 AP2 抄写因素,毛状体和 ARTEMISININ 管理者 1 (TAR1 ) ,在在 A.annua 调整毛状体的发展和 artemisinin 的生合成起关键作用。TAR1,编码特殊位于原子核的蛋白质,主要在年轻叶子,花芽,和一些毛状体被表示。在 TAR1-RNAi 线,毛状体的形态学和表皮的蜡的作文被改变,并且 artemisinin 内容戏剧性地被减少它能被 TAR1 oeverexpression 显著地增加。当 TAR1 是 silenced 或 overexpressed 时,涉及 artemisinin 生合成的几关键基因的表示层次被改变。由 electrophoretic 活动性移动,酵母一个混血儿和短暂转变 -glucuronidase 试金,我们证明广告和 CYP71AV1,在 artemisinin 的生合成小径的二关键基因,是可能的 TAR1 的直接目标。一起拿,我们的结果显示 TAR1 是在 A.annua 调整毛状体开发和 artemisinin 生合成的分子的网络的一个关键部件。 | Hexin Tan Ling Xiao Shouhong Gao Qing Li Junfeng Chen Ying Xiao Qian Ji Ruibing Chen Wansheng Chen Lei Zhang | 2015 | Molecular Plant2015，8，9： | 37 |
| 2 | Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System显示文摘 | Lu, Yuming Ye, Xiao Guo, Renming Huang, Jing Wang, Wei Tang, Jiuyou Tan, Longtao Zhu, Jian-kang Chu, Chengcai Qian, Yangwen | 2017 | Molecular Plant2017，10，9： | 28 |
| 3 | Structure of the African swine fever virus major capsid protein p72显示文摘Dear Editor,African swine fever(ASF)caused by the African swine fever virus(ASFV)infection is a highly contagious and lethal disease of domestic pigs.Recent spread of the virus to China,1 the biggest pork-consuming country,has caused huge economic loss.So far there is no effective way to prevent the spread of the virus.Vaccine for protection from ASFV infection or effective treatments to cure ASF is urgently needed. | Qi Liu Bingting Ma Nianchao Qian Fan Zhang Xu Tan Jianlin Lei Ye Xiang | 2019 | Cell Research2019，29，11： | 28 |
| 4 | Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction显示文摘AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QTAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction.RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×107 and 1.67×107 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.L4×105 and 3.02×105 copies of HBV DNA per mL in the semen.CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. | Wei-Ping Qian Yue-Qiu Tan Ying Chen Ying Peng Zhi Li Guang-Xiu Lu Made C. Liu Hsiang-Fu Kung Ming-Ling He Li-Ka Shing | 2005 | World Journal of Gastroenterology2005，11，34： | 25 |
| 5 | Surveillance, epidemiology, and pathogen spectrum of hand, foot, and mouth disease in mainland of China from 2008 to 2017显示文摘Hand,foot and mouth disease(HFMD)was reported in May 2,2008 to be the 38th legally notifiable disease in China's National Notifiable Disease Reporting and Surveillance System.In order to solve the infection,an extensive three-level HFMD surveillance laboratory network was established.In this study,the framework of that network is assessed and the incidence of HFMD in China from 2008 to 2017 is reported using a descriptive epidemiologic method.During these 10 years,a series of techniques have been widely applied in all the network laboratories.Using information and material obtained from the network,a virus bank and database containing 18,238 viruses were established.Nationally,18,184,834 HFMD cases,including 152,436 severe cases and 3633 fatal cases,were reported in mainland of China.The average annual incidence in the population was 133.99/100,000 people,with a maximum incidence of 205.06/100,000 people in 2014.The incidence and mortality rates of HFMD were the highest in children aged 1–2 years.The numbers of reported cases fluctuated,with a high incidence observed every 2 years.An overall increase in the number of reported cases was also observed throughout the study period.Despite this,the incidence of severe cases and the mortality rate have been decreasing.High-risk regions are located in southern,eastern,and central China.Two peaks of HFMD infection cases were observed annually except for Northeast China.Different proportions of enterovirus serotypes were observed during the studied years.The predominant enterovirus varies from year to year,but the disease severity is always closely related to the specific serotype.EV-A71 is the dominant serotype associated with severe and fatal cases,with constituent ratios of 70.03%and 92.23%,respectively.The studied highly sensitive and efficient surveillance network provides information that is critical for prevention and control of the disease.It is extremely necessary and important to continuously conduct extensive virological surveillance for HFMD. | Tianjiao Ji Taoli Han Xiaojuan Tan Shuangli Zhu Dongmei Yan Qian Yang Yang Song Aili Cui Yan Zhang Naiying Mao Songtao Xu Zhen Zhu Dandan Niu Yong Zhang Wenbo Xu | 2019 | Biosafety and Health2019，1，1： | 26 |
| 6 | Effect of ulinastatin on paraquat-induced-oxidative stress in human type Ⅱ alveolar epithelial cells显示文摘BACKGROUND:Ulinastatin(UTI) is a urinary trypsin inhibitor extracted and purified from urine of males.This study aimed to explore the effects of UTI on paraquat-induced-oxidative stress in human type Ⅱ alveolar epithelial cells.METHODS:The human type II alveolar epithelial cells,A549 cells,were cultured in vitro.The A549 cells were treated with different concentrations of paraquat(200,400,600,800,1 000,1 200 umol/L) and ulinastatin(0,2 000,4 000,6 000,8 000 U/mL) for 24 hours,the cell viability was measured by cell counting kit-8 and the median lethal concentration was selected.In order to establish an in vitro model of paraquat intoxication and to determine the safe dose of ulinastatin,we calculated LD50 using cell counting kit-8 to determine the survival rate of the cells.A549 cells were divided into normal control group,paraquat group and paraquat+ulinastatin group.The levels of malondialdehyde(MDA) and myeloperoxidase(MPO) were detected by biochemistry colorimetry,while the level of reactive oxygen spies(ROS) was detected by DCFH-DA assay.RESULTS:The survival rate of A549 cells treated with different concentrations of paraquat decreased in a concentration-dependent manner.Whereas there was no decrease in the survival rate of cells treated with 0-4 000 U/mL ulinastatin.The levels of MDA,MPO,and ROS were significantly higher in the paraquat group than in the normal control group after 24-hour-exposure.And the survival rate of the paraquat+ulinastatin group was higher than that of the paraquat group,but lower than that of the normal control group.The levels of MDA,MPO,and ROS were lower than those of the paraquat group.CONCLUSION:Ulinastatin can alleviate the paraquat-induced A549 cell damage by reducing oxidative stress. | Xiao-xiao Meng Rui-lan Wang Shan Gao Hui Xie Jiu-ting Tan Yong-bin Qian | 2013 | World Journal of Emergency Medicine2013，4，2： | 24 |
| 7 | Tumor suppressor miR-128-3p inhibits metastasis and epithelial-mesenchymal transition by targeting ZEB1 in esophageal squamous-cell cancer显示文摘MicroRNAs (miRNAs ) 是在 post-transcriptional 层次调整多重生物功能的一些短 RNA,例如 tumorigenic 过程,煽动性的损害和房间 apoptosis。(ZEB1 ) 锌手指电子盒子有约束力的 homeobox 因素 1 是上皮间充质的转变(EMT ) 的一个关键调停人。它包括人的食道的有鳞房间的癌(ESCC ) 导致各种各样的癌症的恶意的前进。在这研究,我们发现 miR-128-3p 由使用 PCR 是在 ESCC 纸巾和房间的 downregulated。而且, miR-128-3p 的下面调整的表示被证明与 ESCC 病人的差的预后被联系并且可能被认为是一个独立预示的因素。然后,我们在 ESCC 房间检验了 miR-128-3p 的角色,并且发现 miR-128-3p 能在 vitro 压制房间迁居和侵略。而且, ZEB1 被证实是由酶记者试金的 miR-128-3p 的一个直接目标。营救实验证明 EMT 被 miR-128-3p 经由 ZEB1 的抑制调整。一起拿所有,我们断定 miR-128-3p 压制 EMT,经由 ZEB1 的转移,和 miR-128-3p 可以是在 ESCC 的一个批评调停人。 | Lili Zhao Rui Li Shanling Xu Yi Li Pei Zhao Wei Dong Zhenjun Liu Qian Zhao Bo Tan | 2018 | Acta Biochimica et Biophysica Sinica2018，50，2： | 21 |
| 8 | Ferroptosis is essential for diabetic cardiomyopathy and is prevented by sulforaphane via AMPK/NRF2 pathways显示文摘Herein,we define the role of ferroptosis in the pathogenesis of diabetic cardiomyopathy(DCM)by examining the expression of key regulators of ferroptosis in mice with DCM and a new ex vivo DCM model.Advanced glycation end-products(AGEs),an important pathogenic factor of DCM,were found to induce ferroptosis in engineered cardiac tissues(ECTs),as reflected through increased levels of Ptgs2 and lipid peroxides and decreased ferritin and SLC7 A11 levels.Typical morphological changes of ferroptosis in cardiomyocytes were observed using transmission electron microscopy.Inhibition of ferroptosis with ferrostatin-1 and deferoxamine prevented AGE-induced ECT remodeling and dysfunction.Ferroptosis was also evidenced in the heart of type 2 diabetic mice with DCM.Inhibition of ferroptosis by liproxstatin-1 prevented the development of diastolic dysfunction at 3 months after the onset of diabetes.Nuclear factor erythroid 2-related factor 2(NRF2)activated by sulforaphane inhibited cardiac cell ferroptosis in both AGE-treated ECTs and hearts of DCM mice by upregulating ferritin and SLC7 A11 levels.The protective effect of sulforaphane on ferroptosis was AMP-activated protein kinase(AMPK)-dependent.These findings suggest that ferroptosis plays an essential role in the pathogenesis of DCM;sulforaphane prevents ferroptosis and associated pathogenesis via AMPK-mediated NRF2 activation.This suggests a feasible therapeutic approach with sulforaphane to clinically prevent ferroptosis and DCM. | Xiang Wang Xinxin Chen Wenqian Zhou Hongbo Men Terigen Bao Yike Sun Quanwei Wang Yi Tan Bradley B.Keller Qian Tong Yang Zheng Lu Cai | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 20 |
| 9 | Precise nanomedicine for intelligent therapy of cancer显示文摘Precise nanomedicine has been extensively explored for efficient cancer imaging and targeted cancer therapy, as evidenced by a few breakthroughs in their preclinical and clinical explorations. Here, we demonstrate the recent advances of intelligent cancer nanomedicine, and discuss the comprehensive understanding of their structure-function relationship for smart and efficient cancer nanomedicine including various imaging and therapeutic applications, as well as nanotoxicity. In particular, a few emerging strategies that have advanced cancer nanomedicine are also highlighted as the emerging focus such as tumor imprisonment, supramolecular chemotherapy, and DNA nanorobot. The challenge and outlook of some scientific and engineering issues are also discussed in future development. We wish to highlight these new progress of precise nanomedicine with the ultimate goal to inspire more successful explorations of intelligent nanoparticles for future clinical translations. | Huabing Chen Zhanjun Gu Hongwei An Chunying Chen Jie Chen Ran Cui Siqin Chen Weihai Chen Xuesi Chen Xiaoyuan Chen Zhuo Chen Baoquan Ding Qian Dong Qin Fan Ting Fu Dayong Hou Qiao Jiang Hengte Ke Xiqun Jiang Gang Liu Suping Li Tianyu Li Zhuang Liu Guangjun Nie Muhammad Ovais Daiwen Pang Nasha Qiu Youqing Shen Huayu Tian Chao Wang Hao Wang Ziqi Wang Huaping Xu Jiang-Fei Xu Xiangliang Yang Shuang Zhu Xianchuang Zheng Xianzheng Zhang Yanbing Zhao Weihong Tan Xi Zhang Yuliang Zhao | 2018 | Science China Chemistry2018，61，12： | 19 |
| 10 | Microarray-Assisted Fine-Mapping of Quantitative Trait Loci for Cold Tolerance in Rice显示文摘包括冷压力抵抗,许多重要农学的特点由量的特点 loci (QTL ) 复杂、控制。这些 QTL 的隔离将极大地有益于农业工业,但是它是一项挑战性的任务。这研究由把 microarray 与印射 QTL 相结合以便在早幼苗的舞台从寒冷容忍的变化 IL112 识别寒冷容忍的 QTL 探索了综合策略。IL112 的所有早幼苗在 45 点为 9 d 通常幸存吗?? | Fengxia Liu Wenying Xu Qian Song Lubin Tan Jiayong Liu Zuofeng zhu Yongcai Fu Zhen Su Chuanqing Sun | 2013 | Molecular Plant2013，6，3： | 17 |
| 11 | Deletion of TLR4 attenuates lipopolysaccharide-induced acute liver injury by inhibiting inflammation and apoptosis显示文摘Septic acute liver injury is one of the leading causes of fatalities in patients with sepsis.Toll-like receptor 4(TLR4)plays a vital role in response to lipopolysaccharide(LPS)challenge,but the mechanisms underlying TLR4 function in septic injury remains unclear.In this study,we investigated the role of TLR4 in LPS-induced acute liver injury(ALI)in mice with a focus on inflammation and apoptosis.Wild-type(WT)and TLR4-knockout(TLR4^(−/−))mice were challenged with LPS(4 mg/kg)for 6 h.TLR4 signaling cascade markers(TLR4,MyD88,and NF-κB),inflammatory markers(TNFα,IL-1β,and IL-6),and apoptotic markers(Bax,Bcl-2,and caspase 3)were evaluated.We showed that LPS challenge markedly increased the levels of serum alanine aminotransferase(ALT)/aspartate aminotransferase(AST)and other liver pathological changes in WT mice.In addition,LPS challenge elevated the levels of liver carbonyl proteins and serum inflammatory cytokines,upregulated the expression of TLR4,MyD88,and phosphorylated NF-κB in liver tissues.Moreover,LPS challenge significantly increased hepatocyte apoptosis,caspase 3 activity,and Bax level while suppressing Bcl-2 expression in liver tissues.These pathological changes were greatly attenuated in TLR4^(−/−)mice.Similar pathological responses were provoked in primary hepatic Kupffer cells isolated from WT and TLR4^(−/−)mice following LPS(1μg/mL,6 h)challenge.In summary,these results demonstrate that silencing of TLR4 attenuates LPS-induced liver injury through inhibition of inflammation and apoptosis via TLR4/MyD88/NF-κB signaling pathway.TLR4 deletion confers hepatoprotection against ALI induced by LPS,possibly by repressing macrophage inflammation and apoptosis. | Sai-nan Chen Ying Tan Xiao-chan Xiao Qian Li Qi Wu You-you Peng Jun Ren Mao-long Dong | 2021 | Acta Pharmacologica Sinica2021，42，10： | 17 |
| 12 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 13 | Seasonal variation of Microcystis in Lake Taihu and its relationships with environmental factors显示文摘In order to monitor the changes of Microcystis along with temporal and spatial variations, seasonal variation of Microcystis in Lake Taihu was investigated by 16S-23S rRNA internal transcribed spacer denaturing gradient gel electrophoresis (16S-23S rRNA-ITS DGGE) and microscopic evaluation. Samples were collected quarterly at four sites (River Mouth, Meiliang Bay, Cross Area, and Lake Center) from August 2006 to April 2007. Results showed that Microcystis dominated total phytoplankton abundance at the four sites in all seasons except winter. The average annual abundance of Microcystis was relatively high at River Mouth and Meiliang Bay, reaching 81.22 × 106 and 61.32 × 106 cells/L, respectively. For temporal variations, Shannon-Wiener diversity index (H ) according to DGGE profile revealed the richness of Microcystis in summer (H = 1.375 ± 0.034) and winter (H = 1.650 ± 0.032) was lower than that in spring (H = 2.078 ± 0.031) and autumn (H = 2.365 ± 0.032) (P < 0.05). While for spatial variations, the richness of Microcystis at River Mouth (H = 2.015 ± 0.074) was higher than at other sites during four seasons (P < 0.01). Very few dierences of Microcystis diversity in the same season were observed among the other three sites (P > 0.05). Canonical correspondence analysis (CCA) was performed to elucidate the relationships between Microcystis operational taxonomic units (OTUs) composition and the environmental factors. Results of CCA revealed that temperature was strongly positively correlated with the first axis (r = 0.963), while TSS was negative correlated with the second axis (r = -0.716). Phylogenetic tree based on the sequencing results of target bands on DGGE gel indicated that samples collected in summer and winter constituted two separated clusters. | TAN Xiao KONG Fanxiang ZENG Qingfei CAO Huansheng QIAN Shanqin ZHANG Min | 2009 | Journal of Environmental Sciences2009，21，7： | 16 |
| 14 | Expression of perforin and granzyme B mRNA in judgement of immunosuppressive effect in rat liver transplantation显示文摘AIM To explore the expression of perform andgranzyme B genes mRNA to judge the effect ofimmunosuppression in acute rejection of livertransplantation.METHODS The expression of perform andgranzyme B genes mRNA was examined byreverse transcriptase-polymerase chain reaction(RT--PCR) in hamster to rat liver grafts under theimmunosuppression of cyclosporine or/andsplenectomy. Histological findings were studiedcomparatively.RESULTS Cyclosporine could obviouslydecrease the cellular infiltration, and completelyrepress the expression of mRNA for perform andgranzyme B, but could not change severehepatocyte necrosis and hemorrhage.SPlenectomy could significantly lightenhepatocyte necrosis, and completely eliminatehemorrhage, but not atfect the cellularinfiltration and the expression of perform andgranzyme B genes mRNA. Cyclosporine orsplenectomy alone could not prolong theSurvival time, however, their combination couldcompletely repress the rejection of liver grafts.The survival time of animals were significantlyprolonged (37.1 days ). The architecture ofhepatic lobules was preserved. There was slightcellular infiltration in the portal tracts and noexpression of perform and granzyme B genesmRNA could be seen in three weeks aftertransplantation.CONCLUSION Perform and granzyme B genesare valuable in judging the effect ofimmunosuppression in liver transplantation. | Zhang SG Wu MC Tan JW Chen H Yang JM Qian QJ | 1999 | World Journal of Gastroenterology1999，5，3： | 15 |
| 15 | The herbal compound geniposide rescues formaldehyde-induced apoptosis in N2a neuroblastoma cells显示文摘The herbal medicine Tong Luo Jiu Nao(TLJN)contains geniposide(GP)and ginsenoside Rg1 at a molar ratio of 10:1.Rg1 is the major component of another herbal medicine,panax notoginseng saponin(PNS).TLJN has been shown to strengthen brain function in humans,and in animals it improves learning and memory.We have previously shown that TLJN reduces amyloidogenic processing in Alzheimer’s disease(AD)mouse models.Together this suggests TLJN may be a potential treatment for patients with dementia.Because chronic damage of the central nervous system by formaldehyde(FA)has been presented as a risk factor for age-associated cognitive dysfunction,in the present study we investigated the protective effect of both TLJN and GP in neuron-like cells exposed to FA.FA-exposed murine N2a neuroblastoma cells were incubated with TLJN,its main ingredient GP,as well as PNS,to measure cell viability and morphology,the rate of apoptosis and expression of genes encoding Akt,FOXO3,Bcl2 and p53.The CCK-8 assay,cytoskeletal staining and flow cytometry were used to test cell viability,morphology and apoptosis,respectively.Fluorescent quantitative real-time PCR(qRT-PCR)was used to monitor changes in gene expression,and HPLC to determine the rate of FA clearance.Treatment of N2a cells with 0.09 mmol L?1 FA for 24 h significantly reduced cell viability,changed cell morphology and promoted apoptosis.Both TLJN and GP conferred neuroprotection to FA-treated N2a cells,whereas PNS,which had to be used at lower concentrations because of its toxicity,did not.Our data demonstrate that TLJN can rescue neuronal damage caused by FA and that its main ingredient,GP,has a major role in this efficacy.This presents purified GP as a drug or lead compound for the treatment of AD. | CHEN JinYan SUN MengRu WANG XingHua LU Jing WEI Yan TAN Yan LIU Ying GTZ Jürgen HE RongQiao HUA Qian | 2014 | Science China(Life Sciences)2014，57，4： | 15 |
| 16 | Serum hepatitis B virus RNA is a predictor of HBeAg seroconversion and virological response with entecavir treatment in chronic hepatitis B patients显示文摘BACKGROUND Characteristics of alterations of serum hepatitis B virus(HBV) RNA in different chronic hepatitis B(CHB) patients still cannot be fully explained. Whether HBV RNA can predict HBeAg seroconversion is still controversial.AIM To investigate whether HBV RNA can predict virological response or HBeAg seroconversion during entecavir(ETV) treatment when HBV DNA is undetectable.METHODS The present study evaluated 61 individuals who were diagnosed and treated with long-term ETV monotherapy at the Department of Infectious Diseases of Peking University First Hospital(China) from September 2006 to December 2007.Finally, 30 treatment-naive individuals were included. Serum HBV RNA were extracted from 140 μL serum samples at two time points. Then they were reverse transcribed to cDNA with the HBV-specific primer. The product was quantified by real-time quantitative PCR(RT-PCR) using TAMARA probes. Statistical analyses were performed with IBM SPSS 20.0.RESULTS Level of serum HBV RNA at baseline was 4.15 ± 0.90 log10 copies/mL. HBV RNA levels showed no significant difference between the virological response(VR)and partial VR(PVR) groups at baseline(P = 0.940). Serum HBV RNA significantly decreased among patients who achieved a VR during ETV therapy(P < 0.001). The levels of HBV RNA in both HBeAg-positive patients with seroconversion group and those with no seroconversion increased after 24 wk of treatment. Overall, HBV RNA significantly but mildly correlated to HBsAg(r =0.265, P = 0.041), and HBV RNA was not correlated to HBV DNA(r = 0.242, P =0.062). Furthermore, serum HBV RNA was an independent indicator for predicting HBeAg seroconversion and virological response. HBeAg seroconversion was more likely in CHB patients with HBV RNA levels below4.12 log10 copies/mL before treatment.CONLUSION The level of serum HBV RNA could predict HBeAg seroconversion and PVR during treatment. In the PVR group, the level of serum HBV RNA tends to be increasing. | Hao Luo Xia-Xia Zhang Li-Hua Cao Ning Tan Qian Kang Hong-Li Xi Min Yu Xiao-Yuan Xu | 2019 | World Journal of Gastroenterology2019，25，6： | 15 |
| 17 | Proteome analysis of hepatocellular carcinoma by laser capture microdissection显示文摘 | Ai J Tan Y Ying W Hong Y Liu S Wu M Qian X Wang H | 2006 | 第二军医大学学报2006，27，5： | 14 |
| 18 | Direct reprogramming of human fibroblasts into dopaminergic neuron-like cells显示文摘外长的 dopaminergic 神经原(DA 神经原) 的移植是为治疗 Parkinson 的疾病(PD ) 的一条有希望的途径。然而,主要跌倒块是施主 DA 神经原的可靠来源的缺乏。这里,我们证明五 transcriptional 因素 Mash1, Ngn2, Sox2, Nurr1,和 Pitx3 的联合直接并且有效地装改编人成纤维细胞进 DA 像神经原的房间。为为 DA 神经原的各种各样的标记积极的染色的 reprogrammed 房间。他们也显示出典型 DA 举起和生产性质。而且,他们展出了 DA 神经原特定的 electrophysiological 侧面。最后,他们在一个老鼠 PD 模型提供了征兆的地势。因此,我们的直接 reprogrammed DA 像神经原的房间是为 PD 的房间代替治疗的有希望的来源。 | Xinjian Liu Fang Li Elizabeth A Stubblefield Barbara Blanchard Toni L Richards Gaynor A Larson Yujun He Qian Huang Aik-Choon Tan Dabing Zhang Timothy A Benke John R Sladek Nancy R Zahniser Chuan-Yuan Li | 2012 | Cell Research2012，22，2： | 14 |
| 19 | Preoperative Corticosteroid Usage and Hypoalbuminemia Increase Occurrence of Short-term Postoperative Complications in Chinese Patients with Ulcerative Colitis显示文摘 | Ji Li Hong Lyu Hong Yang Yue Li Bei Tan Ming-Ming Wei Xi-Yu Sun Jing-Nan Li Bin Wu Jia-Ming Qian | 2016 | Chinese Medical Journal2016，，4： | 14 |
| 20 | Management of granulomatous lobular mastitis: an international multidisciplinary consensus(2021 edition)显示文摘Granulomatous lobular mastitis(GLM) is a rare and chronic benign inflammatory disease of the breast. Difficulties exist in the management of GLM for many front-line surgeons and medical specialists who care for patients with inflammatory disorders of the breast. This consensus is summarized to establish evidence-based recommendations for the management of GLM. Literature was reviewed using PubMed from January 1, 1971 to July 31, 2020. Sixty-six international experienced multidisciplinary experts from 11 countries or regions were invited to review the evidence.Levels of evidence were determined using the American College of Physicians grading system, and recommendations were discussed until consensus. Experts discussed and concluded 30 recommendations on historical definitions,etiology and predisposing factors, diagnosis criteria, treatment, clinical stages, relapse and recurrence of GLM. GLM was recommended as a widely accepted definition. In addition, this consensus introduced a new clinical stages and management algorithm for GLM to provide individual treatment strategies. In conclusion, diagnosis of GLM depends on a combination of history, clinical manifestations, imaging examinations, laboratory examinations and pathology.The approach to treatment of GLM should be applied according to the different clinical stage of GLM. This evidencebased consensus would be valuable to assist front-line surgeons and medical specialists in the optimal management of GLM. | Qian-Qian Yuan Shu-Yuan Xiao Omar Farouk Yu-Tang Du Fereshte Sheybani Qing Ting Tan Sami Akbulut Kenan Cetin Afsaneh Alikhassi Rami Jalal Yaghan Irmak Durur-Subasi Fatih Altintoprak Tae Ik Eom Fatih Alper Mustafa Hasbahceci David Martínez-Ramos Pelin Seher Oztekin Ava Kwong Cedric W.Pluguez-Turul Kirstyn EBrownson Shirish Chandanwale Mehran Habib Liu-Yi Lan Rui Zhou Xian-Tao Zeng Jiao Bai Jun-Wen Bai Qiong-Rong Chen Xing Chen Xiao-Ming Zha Wen-Jie Dai Zhi-Jun Dai Qin-Yu Feng Qing-Jun Gao Run-Fang Gao Bao-San Han Jin-Xuan Hou Wei Hou Hai-Ying Liao Hong Luo Zheng-Ren Liu Jing-Hua Lu Bin Luo Xiao-Peng Ma Jun Qian Jian-Yong Qin Wei Wei Gang Wei Li-Ying Xu Hui-Chao Xue Hua-Wei Yang Wei-Ge Yang Chao-Jie Zhang Fan Zhang Guan-Xin Zhang Shao-Kun Zhang Shu-Qun Zhang Ye-Qiang Zhang Yue-Peng Zhang Sheng-Chu Zhang Dai-Wei Zhao Xiang-Min Zheng Le-Wei Zheng Gao-Ran Xu Wen-Bo Zhou Gao-Song Wu | 2022 | Military Medical Research2022，9，4： | 13 |
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