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| 1 | One-year clinical study of NeuroR egen scaffold implantation following scar resection in complete chronic spinal cord injury patients显示文摘The objective of this clinical study was to assess the safety and feasibility of the collagen scaffold, Neuro Regen scaffold, one year after scar tissue resection and implantation. Scar tissue is a physical and chemical barrier that prevents neural regeneration. However, identification of scar tissue is still a major challenge. In this study, the nerve electrophysiology method was used to distinguish scar tissue from normal neural tissue, and then different lengths of scars ranging from 0.5–4.5 cm were surgically resected in five complete chronic spinal cord injury(SCI) patients. The NeuroR egen scaffold along with autologous bone marrow mononuclear cells(BMMCs), which have been proven to promote neural regeneration and SCI recovery in animal models, were transplanted into the gap in the spinal cord following scar tissue resection. No obvious adverse effects related to scar resection or Neuro Regen scaffold transplantation were observed immediately after surgery or at the 12-month follow-up. In addition, patients showed partially autonomic nervous function improvement, and the recovery of somatosensory evoked potentials(SSEP) from the lower limbs was also detected. The results indicate that scar resection and Neuro Regen scaffold transplantation could be a promising clinical approach to treating SCI. | Zhifeng Xiao Fengwu Tang Jiaguang Tang Huilin Yang Yannan Zhao Bing Chen Sufang Han Nuo Wang Xing Li Shixiang Cheng Guang Han Changyu Zhao Xiaoxiong Yang Yumei Chen Qin Shi Shuxun Hou Sai Zhang Jianwu Dai | 2016 | Science China(Life Sciences)2016，59，7： | 18 |
| 2 | 生物反馈治疗高血压临床疗效剖析显示文摘对89例Ⅰ/Ⅱ期高血压病人经一年的生物反馈治疗,发现疗效受多种生物心理社会因素的影响,其中收缩压稍低、高神经质(情绪不稳定)、愤怒分值和焦虑分值较高,以及社会支持程度较高的病人其治疗效果较好。 | Zhang Sufang et al, Depart-ment of Medical Psychology, Beijing Medical University. Beijing 100083 | 1995 | 中国心理卫生杂志1995，9，4： | 8 |
| 3 | Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry显示文摘The aim of this work was to develop an automated on-line solid phase extraction(SPE)with liquid chromatography-tandem mass spectrometry method for the detection of fifteen sulfonamides in pork and fish samples.Samples were extracted with 0.2%formic acid acetonitrile solution,purified by on-line SPE device with HLB column,then separated by XBridge C18 column,using 0.1%formic acid solution and acetonitrile as the mobile phase.Mass spectrometric data was acquired under multiple reaction monitoring(MRM)mode using positive ionization electrospray.Internal standard method was used in the quantification,good linear relationship was got in range of 0.1–100 ng/mL and correlation coefficient was higher than 0.9990.The limits of detection were in the range of 0.125–2.00g/kg and the limits of quantitation were in the range of 0.250–5.00g/kg.Recoveries of the method were in range of 78.3%–99.3%,relative standard deviation were lower than 10%.The method was simple,sensitivity,and could be used for routine supervision and analysis of fifteen sulfonamides in pork and fish. | Junmei Ma Sufang Fan Lei Sun Liangna He Yan Zhang Qiang Li | 2020 | Food Science and Human Wellness2020，9，4： | 6 |
| 4 | Simultaneous determination of 15 pesticide residues in Chinese cabbage and cucumber by liquid chromatography-tandem mass spectrometry utilizing online turbulent flow chromatography显示文摘In this experiment,a liquid chromatography tandem mass spectrometry method was built to determine 15 pesticide residues in Chinese cabbage and cucumber samples based on online turbulent flow chromatography purification.After modified quick,easy,cheap,effective,rugged,and safe(QuEChERS)extraction,extracts were directly injected to the TLX(TurboFlow Liquid Xcalibur)system and brought to TurboFlow™columns for on-line purification and then transferred to analytical column for further separation and analysis.TurboFlow™columns types,transfer flow rate,and transfer time were optimized.Limits of detection and limits of quantification of the method obtained for 15 pesticide residues were ranged between 0.2–1.0μg/kg and 0.5–2.0μg/kg in Chinese cabbage and cucumber samples.Recoveries of pesticide residues were in range of 75.3%–103.7%.Matrix effects for 15 pesticides were in range of 5.6%–106.6%.The developed method has been successfully used for the determination of 15 pesticide residues in real samples. | Sufang Fan Junmei Ma Meirong Cao Juan Wang Leilei Zhang Yan Zhang Qiang Li Jia Chen | 2021 | Food Science and Human Wellness2021，10，1： | 5 |
| 5 | Effects of lanthanum on the growth and essential oil components of lavender under osmotic stress显示文摘The effects of lanthanum on growth, soluble sugar content, essential oil contents and quality were studied in lavender plant(Lavandula Angustifolia Mill, Variety 701) under osmotic stress by means of PEG-6000 solution. The results show that osmotic stress could reduce the growth rate of lavender, and increase the content of SSC(soluble sugar concentration) to some extent. Compared with control group,the contents of essential oil in flowers and leaves respectively increase by 45.6% and 48.3% in the osmotic stress group induced by 15% PEG-6000. However, the presence of lanthanum can make the contents of essential oil in the flowers and leaves of stressed lavender plants enhance by 19.4% and 18.6%, respectively. Under osmotic stress, the relative contents of four kinds of lavender essential oil compositions of camphor, linalool, linalyl acetate and lavandulol acetate in the lavender flowers were successively 22.63%,0.61%,25.46%,7.03%,and 20.17%,0.62%,20.72%,10.80%,respectively in the presence and absence of lanthanum. The contents of all four main components of lavender essential oils meet the requirements of the national standard under osmotic stress in the presence of lanthanum. However, in the absence of lanthanum, the contents of linalyl acetate and lavandulol acetate does not meet the requirements of the national standard under osmotic stress. Moreover, the contents of five components of borneol, camphor,eudesmol, caryophyllene oxide and bicyclic sesquiphellandrene from the essential oil of lavender leaves are 12.89%, 3.84%, 8.76%, 11.30% and 8.10%, respectively. The total content of above five components accounts for 44.89% of the essential oil of the lavender leaves. Particularly, the borneol content in leaf essential oil is up to 12.89%. It is 125.2 times the amount of borneol in the flower essential oil. In conclusion, the suitable concentration of lanthanum can improve the adaptability of lavender plants, and heighten the content and quality of lavender essential oil to some extent under osmotic stress. | Liyun Zhu Linzhen Song Yongsheng Gao Junqing Qian Xiaodong Zhang Sufang Li | 2018 | Journal of Rare Earths2018，36，8： | 5 |
| 6 | Annual Report of SGCC on Transactions of Electric Power Market in 2009显示文摘Market construction Overview In 2009, the electric power market expanded continuously. New installed capacity put into production within the coverage of the State Grid Corporation | Electricity Transaction Center, State Grid Corporation of China Zhang Sufang | 2010 | Electricity2010，21，2： | 4 |
| 7 | Research on protoplast preparation and culture in Gladiolus hybridus Hort显示文摘Protoplasts prepared from the aseptic leaves of Rose Supreme in Gladiolus hybridus Hort. were cultured. The yield of protoplasts with vigour was the highest under the following conditions: 1.5% cellulase Onzuka RS, 0.5% pectinase Y-23, 0.6 mol· L^(-1) mannitol, pH 5.8, digestion time 2 h, and temperature 27℃. After isolation and purification, protoplasts were cultured in solid and liquid combined medium, which included KM8P, 0.6 mol· L^(-1) mannitol, 1.0 mg· L^(-1) 2, 4-D, 0.2 mg· L^(-1) NAA and 0.2 mg· L^(-1) KT. The division of the protoplasts first emerged in medium after cultured 4 days, and emerged only a few times. | ZHANG Ying GONG Sufang WANG Jingang CHE Daidi | 2007 | Journal of Northeast Agricultural University(English Edition)2007，14，1： | 2 |
| 8 | Moderate papain addition improves the physicochemical,microbiological,flavor and sensorial properties of Chouguiyu,traditional Chinese fermented fish显示文摘The objective of this study was to investigate the effect of papain on the physicochemical and microbiological properties,volatile compound profiles,and sensorial attributes of Chouguiyu.Papain was applied at a dose of 10,50,200,400 U/g fish.Results showed that papain addition improved the release of free amino acids,essential amino acids,eicosapentaenoi acid(EPA,C20:5n3),and docosapentenoic acid(DPA,C22:5n6),respectively.Lower biogenic amines content,higher the volatile compound concentrations and relative abundance of Lactobacillales were also observed.Further results showed that the papain dose group decreased the fermentation time from 10 to 7 days while good organoleptic quality was remained,especially in the 200 U/g dose group.The redundancy analysis(RDA)showed a high correlation between Lactobacillus and the key volatile compounds in the 200 U/g and 400 U/g groups,which indicated that Lactobacillus could contribute to the fatty flavor.In summary,proper papain addition(i.e.200 U/g or 400 U/g)could reduce biogenic amines contents and maintain acceptable sensorial properties of Chouguiyu in a shorter fermentation time. | Wenhuan Xu Cuicui Jiang Aoxue Liu Ruiqi Bao Wenqing Wang Zuoli Zhang Chaofan Ji Huipeng Liang Sufang Zhang Xinping Lin | 2022 | Food Bioscience2022，46，2： | 2 |
| 9 | Removal of aflatoxin B 1 in edible plant oils by oscillating treatment with alkaline electrolysed water显示文摘 | Sufang Fan Fengzu Zhang Shaowen Liu Chuanshan Yu Di Guan Canping Pan | 2013 | Food Chemistry2013，，3： | 2 |
| 10 | Political and institutional analysis of the successes and failures of China’s wind power policy显示文摘 | Sufang Zhang Philip Andrews-Speed Xiaoli Zhao | 2013 | Energy Policy2013，，： | 2 |
| 11 | Antrodia cinnamomea exerts an anti-hepatoma effect by targeting PI3K/AKT-mediated cell cycle progression in vitro and in vivo显示文摘Antrodia cinnamomea is extensively used as a traditional medicine to prevention and treatment of liver cancer.However,its comprehensive chemical fingerprint is uncertain,and the mechanisms,especially the potential therapeutic target for anti-hepatocellular carcinoma(HCC)are still unclear.Using UPLC-Q-TOF/MS,139 chemical components were identified in A.cinnamomea dropping pills(ACDPs).Based on these chemical components,network pharmacology demonstrated that the targets of active components were significantly enriched in the pathways in cancer,which were closely related with cell proliferation regulation.Next,HCC data was downloaded from Gene Expression Omnibus database(GEO).The Cancer Genome Atlas(TCGA)and Dis Ge NET were analyzed by bioinformatics,and 79 biomarkers were obtained.Furtherly,nine targets of ACDP active components were revealed,and they were significantly enriched in PI3 K/AKT and cell cycle signaling pathways.The affinity between these targets and their corresponding active ingredients was predicted by molecular docking.Finally,in vivo and in vitro experiments showed that ACDPs could reduce the activity of PI3 K/AKT signaling pathway and downregulate the expression of cell cycle-related proteins,contributing to the decreased growth of liver cancer.Altogether,PI3 K/AKT-cell cycle appears as the significant central node in anti-liver cancer of A.Cinnamomea. | Yan Zhang Pin Lv Junmei Ma Ning Chen Huishan Guo Yan Chen Xiaoruo Gan Rong Wang Xuqiang Liu Sufang Fan Bin Cong Wenyi Kang | 2022 | Acta Pharmaceutica Sinica B2022，12，2： | 2 |
| 12 | Establishment and Application of a SYBR Green I Real-time Quantitative PCR Assay for the Detection of LAT Gene of Pseudorabies Virus in Bama Miniature Pigs显示文摘[Objective] This study aimed to establish a rapid,sensitive and specific SYBR Green I real-time quantitative PCR assay for the detection of latent pseudorabies virus( PRV) infection. [Method]SYBR Green I real-time quantitative PCR conditions and system for early detection of latent pseudorabies virus infection were optimized and compared with conventional PCR to investigate the sensitivity and specificity. Subsequently,the established assay was applied to detect different clinical samples. [Result]The sensitivity of SYBR Green I real-time quantitative PCR assay( 52 copies / μl) was 1 000 times higher than that of conventional PCR( 5. 2 × 104 copies / μl) and the detection time was shortened by 1 /2. The established assay could be used to detect PRV but could not be used to detect PCV2,PPV,CSFV or PRRSV. Various tissues were collected from Bama miniature pigs with latent PRV infection under sterile conditions for real-time PCR detection. Results showed that viral copy number in the brain,nasal swab,inguinal lymph node,liver,lung and spleen was above 20,while PRV was not detected in the kidney and heart tissues. [Conclusion]The established SYBR Green I real-time quantitative PCR assay for PRV LAT detection was specific,sensitive and rapid,which could be used for pathogen monitoring,epidemiological investigation and quantitative study of PRV. | Sufang LU Guangjun GUO Ling MO Feng WEI Bingmei DONG Wentong ZHANG Zhiqiang SHEN | 2015 | Agricultural Biotechnology2015，4，3： | 2 |
| 13 | Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry显示文摘A new method for screening and identification 420 pesticide residues in fruits and vegetables by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS)were developed.The samples were extracted with acetonitrile/acetic acid(99:1,V/V),and clean-up by SinChERS-Nano(single-step,cheap,effective,rugged,safe,nano)column,determined by UPLC-Q-TOF/MS.The accurate mass database and MS/MS database which contains 420 pesticides were established,the automatic retrieval of detection results was carried on according to the accurate mass,retention time,isotope ratio,ion fragment information,and so on.Method verification was performed on leeks samples.The results showed that 420 pesticides had good linearity in the range of 0.1-100μg/L,and the correlation coeffificients(R2)was greater than 0.990.The limits of detections(LODs)and limits of quantifications(LOQs)of 420 pesticides were in range of 0.05-2.0 and 0.1-5.0μg/L,respectively.The average spike recoveries at 3 levels were 70.1%to 119.7%,and the relative standard deviations(RSD)were lower than 20%(n=6).With this method,a survey of pesticide residues was conducted for 110 samples of 10 different fruits and vegetables,which provided scientific data for ensuring pesticide residue safety of the fruits and vegetables consumed daily by the public.This method was simple,sensitive and accurate,and could be used for rapid screening of 420 pesticide residues in fruits and vegetables. | Junmei Ma Sufang Fan Liqiong Yang Liangna He Hongwen Zhai Xiaowei Ren Qiang Li Yan Zhang | 2023 | Food Science and Human Wellness2023，12，4： | 2 |
| 14 | Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq, and cross-amplifi cation to related species显示文摘Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this ?shery resources. The recently developed speci?c-locus ampli?ed fragment sequencing(SLAF-seq) is an effcient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites(mono-to hexa-nucleotide repeats) were identi?ed using SLAF-seq technology, of which di-nucleotide was the most frequent(13 590, 47.02%), followed by mono-nucleotide(8 138, 28.15%), tri-nucleotide(5 727, 19.81%), tetra-nucleotide(1 104, 3.82%), pentanucleotide(234, 0.81%), and hexa-nucleotide(112, 0.39%). One hundred and thirty-two microsatellite loci(di-and tri-nucleotide) were randomly selected for ampli?cation and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity( Ho) and expected heterozygosity( He) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and0.836, respectively. The polymorphism information content(PIC) ranged from 0.341 to 0.941(mean=0.806).However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in ?ve phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-ampli?ed in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis.The present microsatellites provided the ?rst known set of microsatellite DNA markers for D. maruadsi,D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the ?sheries management formulation and implementation of the understudied species. | NIU Sufang ZHAI Yun WU Renxie ZHANG Haoran TIAN Letian DENG Jiaxin XIAO Yao | 2019 | Journal of Oceanology and Limnology2019，37，1： | 2 |
| 15 | Expression of Pseudorabies Virus gE Core Epitopes in Escherichia coli Strain BL21 and Utilization of Indirect PRV gE-ELISA显示文摘Pseudorabies virus glycoprotein E( PRV gE) has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce gE antigen in large quantities and at low cost,a gene fragment encoding PRV gE core epitopes was expressed in E. coli BL21 expression system. SDS-PAGE and Western Blotting revealed that the expression product in culture supernatant of E. coli BL21 was a recombinant protein,approximately 51. 8 Kd. At 5 h post-induction,protein concentration assay showed that the expression product amounted to 1. 65 mg /ml,accounting for 24. 17% of total proteins in the culture supernatant. An indirect PRV gE-ELISA was established by using the recombinant expression product as a coating antigen. Cross-reactivity assay showed that this antigen was PRV specific. In addition,the assay was consistently reproducible. Comparison of detection results of 240 serum samples between PRV gE-ELISA and a commercially available PRV diagnostic kit showed that there was no significant difference between these two methods( P > 0. 05). | Guangjun GUO Sufang LU Guanggang QU Feng LI Lin DONG Yanli BI Jinliang WANG Feng WEI Na TANG Chunling ZHANG Zhuang DING Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
| 16 | Political and Institutional Analysis of the Successes and Failures of China's Wind Power Policy显示文摘 | Sufang Zhang P A | | 0，，： | 1 |
| 17 | Gankyrin plays an essential role in estrogen-driven and GPR30-mediated endometrial carcinoma cell proliferation via the PTEN/PI3K/AKT signaling pathway显示文摘 | Jiawen Zhang Yongbin Yang Zhenbo Zhang Yinyan He Zhiqiang Liu Yinhua Yu Sufang Wu Bin Cai Youji Feng | 2012 | Cancer Letters2012，，： | 1 |
| 18 | Melittin inhibited glycolysis and induced cell apoptosis in cisplatinresistant lung adenocarcinoma cells via TRIM8显示文摘Chemotherapy is widely used for non-small cell lung cancer(NSCLC)patients at a late stage;however,NSCLC patients often acquire resistance to chemotherapeutic drugs,thus limiting the therapy efficacy.Melittin,a major component of bee venom,possesses anti-tumor activity in various cancer cells.Here,we examined the effects of melittin on A549/DDP cisplatin-resistant lung adenocarcinoma cells and xenografts formed from this cell line and investigated the possible target of melittin.Treatment with melittin resulted in the induction of cell apoptosis,glycolysis inhibition,and reduction of phosphorylated AKT(p-AKT)in A549/DDP cells.We also identified that tripartite motif-containing 8(TRIM8)was a potential target of melittin.Moreover,we found that TRIM8 mRNA expression was elevated in NSCLC specimens as compared to adjacent normal tissues(N=25)and that patients with high expression of TRIM8 had a poor prognosis for lung adenocarcinoma.The knockdown of TRIM8 had a similar effect of melittin,while overexpression of TRIM8 reversed the effects of melittin in A549/DDP cells.More importantly,we revealed that melittin enhanced cisplatin sensitivity in A549/DDP cells and tumor growth in vivo using a xenograft model of A549/DDP cells.In conclusion,melittin appears to be a potential chemotherapy sensitization agent in NSCLC. | SUFANG ZHANG XIANG LV LI LI YINGBIN LUO HUINAN XIANG LIXIN WANG YAN LI | 2021 | BIOCELL2021，45，1： | 1 |
| 19 | Frequency domain methods for the solutions of N-order fuzzy differential equation显示文摘 | Zhang Yue Wang Guangyuan Liu Sufang | 1998 | Fuzzy Sets and Systems1998，94，1： | 1 |
| 20 | The Protein Levels of MCM7 and p63 in Evaluating Lesion Severity of Cervical Disease显示文摘 | Jiawen Zhang Li Wang Min Qiu Zhiqiang Liu Wenyan Qian Yongbin Yang Sufang Wu Youji Feng | 2013 | International Journal of Gynecological Cancer2013，，2： | 1 |
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