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| 1 | Research progress on silicon/carbon composite anode materials for lithium-ion battery显示文摘Silicon(Si) has been considered as one of the most promising anode material for the next generation lithium-ion batteries(LIBs) with high energy densities, due to its high theoretical capacity, abundant availability and environmental friendliness. However, silicon materials with low intrinsic electric and ionic conductivity suffer from huge volume variation during lithiation/delithiation processes leading to the pulverization of Si and subsequently resulting in severe capacity fading of the electrodes. Coupling of Si with carbon(C) realizes a favorable combination of the two materials properties, such as high lithiation capacity of Si and excellent mechanical and conductive properties of C, making silicon/carbon composite(Si/C) ideal candidates for LIBs anodes. In this review, recent progresses of Si/C materials utilized in LIBs are summarized in terms of structural design principles, material synthesis methods, morphological characteristics and electrochemical performances by highlighting the material structures. The mechanisms behind the performance enhancement are also discussed. Moreover, other factors that affect the performance of Si/C anodes, such as prelithiation, electrolyte additives, and binders, are also discussed. We aim to present a full scope of the Si/C-based anodes, and help understand and design future structures of Si/C anodes in LIBs. | Xiaohui Shen Zhanyuan Tian Ruijuan Fan Le Shao Dapeng Zhang Guolin Cao Liang Kou Yangzhi Bai | 2018 | Journal of Energy Chemistry2018，27，4： | 29 |
| 2 | Review of the characteristics and graded utilisation of coal gasification slag显示文摘The characteristics of the energy structure of rich coal,less oil and less gas,coupling with a high external dependence on oil and natural gas and the emphasis on the efficient and clean utilisation of coal,have brought opportunities for coal chemical industry.However,with the large-scale popularisation of coal gasification technology,the production and resulting storage of coal gasification slag continue to increase,which not only result in serious environmental pollution and a waste of terrestrial resources,but also seriously affect the sustainable development of coal chemical enterprises.Hence,the treatment of coal gasification slag is extremely important.In this paper,the production,composition,morphology,particle size structure and water holding characteristics of coal gasification slag are introduced,and the methods of carbon ash separation of gasification slag,both domestically and abroad,are summarised.In addition,the paper also summarises the research progress on gasification slag in building materials,ecological restoration,residual carbon utilisation and other high-value utilisation,and ultimately puts forward the idea of the comprehensive utilisation of gasification slag.For large-scale consumption to solve the environmental problems of enterprises and achieve high-value utilisation to increase the economic benefits of enterprises,it is urgent to zealously design a reasonable and comprehensive utilisation technologies with simple operational processes,strong adaptability and economic benefits. | Xiaodong Liu Zhengwei Jin Yunhuan Jing Panpan Fan Zhili Qi Weiren Bao Jiancheng Wang Xiaohui Yan Peng Lv Lianping Dong | 2021 | Chinese Journal of Chemical Engineering2021，34，7： | 21 |
| 3 | Safety Research in Traditional Chinese Medicine: Methods, Applications, and Outlook显示文摘Traditional Chinese medicine (TCM) is a medical system that has collected and summarized abundant clinical experience over its long history of more than 2000 years. However, the frequent occurrence of TCM-induced adverse reactions has hindered the modernization and internationalization of TCM, while attracting increasing attention from around the world. Unlike chemical drugs and biological agents, the difficulties involved in research on the toxicity and safety of TCM mainly include the complexity of its components and the unpredictability of drug–body interactions. Much of TCM, which has overall therapeutic effects, has the typical mechanisms of multiple components, multiple pathways, and multiple targets. While considering the gradualness and unpredictability of TCM toxicity, the ambiguity of toxicants and safe dosage, and individual differences during long-term TCM administration, we have systematically established key techniques for the toxicity assessment of TCM. These techniques mainly include TCM toxicity discovery in an early phase, based on a combination of drug toxicology genomics and metabolomics;methods to identify dose–toxicity relationships in TCM;and integrated techniques for the exploration of TCM interactions, such as fast-screening tests based on drug-metabolizing enzymes and receptor pathways. In particular, we have developed a new technical system for TCM safety evaluation using molecular toxicology, which has been validated well in research on TCM compatibility contraindication, quality control, and allergen discovery. The application of this key technical platform is introduced here in detail. This application includes model organisms, toxicant biomarkers, a magnetic suspension technique, and the application of network toxicology and computational toxicology in research on the toxicity of Fructus toosendan, Semen cassiae, Polygonum multiflorum, and Fructus psoraleae. | Yue Gao Aihua Liang Xiaohui Fan Limin Hu Feiran Hao Yubo Li | 2019 | Engineering2019，5，1： | 19 |
| 4 | Giant photonic spin Hall effect in momentum space in a structured metamaterial with spatially varying birefringence显示文摘The photonic spin Hall effect(SHE)in the reflection and refraction at an interface is very weak because of the weak spin-orbit interaction.Here,we report the observation of a giant photonic SHE in a dielectric-based metamaterial.The metamaterial is structured to create a coordinate-dependent,geometric Pancharatnam–Berry phase that results in an SHE with a spin-dependent splitting in momentum space.It is unlike the SHE that occurs in real space in the reflection and refraction at an interface,which results from the momentum-dependent gradient of the geometric Rytov–Vladimirskii–Berry phase.We theorize a unified description of the photonic SHE based on the two types of geometric phase gradient,and we experimentally measure the giant spin-dependent shift of the beam centroid produced by the metamaterial at a visible wavelength.Our results suggest that the structured metamaterial offers a potential method of manipulating spin-polarized photons and the orbital angular momentum of light and thus enables applications in spin-controlled nanophotonics. | Xiaohui Ling Xinxing Zhou Xunong Yi Weixing Shu Yachao Liu Shizhen Chen Hailu Luo Shuangchun Wen Dianyuan Fan | 2015 | Light(Science & Applications)2015，4，1： | 15 |
| 5 | IL-10-producing regulatory B cells restrain the T follicular helper cell response in primary Sjögren’s syndrome显示文摘Increased numbers of T follicular helper(Tfh)cells have been implicated in the development of autoimmune diseases including primary Sjögren’s syndrome(pSS),but how the Tfh cell response is regulated during autoimmune pathogenesis remains largely unclear.Here,we first found negative correlations between IL-10^(+)regulatory B(Breg)cell numbers and Tfh cell responses and disease activity in patients with pSS and mice with experimental Sjögren’s syndrome(ESS).Moreover,we detected high expression of IL-10 receptor on Tfh cells and their precursors in both humans and mice.In culture,IL-10 suppressed human and murine Tfh cell differentiation by promoting STAT5 phosphorylation.By using an adoptive transfer approach and two-photon live imaging,we found significantly increased numbers of Tfh cells with enhanced T cell homing into B cell follicles in the draining cervical lymph nodes of RAG-2−/−mice transferred with IL-10-deficient B cells during ESS development compared with those of RAG-2−/−mice transferred with wild-type B cells.In ESS mice,CD19^(+)CD1d^(hi)CD5^(+)Breg cells with decreased IL-10 production exhibited severely impaired suppressive effects on T cell proliferation.Consistently,CD19^(+)CD24^(+)CD38^(hi) Breg cells from pSS patients showed significantly reduced IL-10 production with defective inhibitory function in the suppression of autologous Tfh cell expansion.Furthermore,the adoptive transfer of IL-10-producing Breg cells markedly suppressed the Tfh cell response and ameliorated ESS progression in ESS mice.Together,these findings demonstrate a critical role for IL-10-producing Breg cells in restraining the effector Tfh cell response during pSS development. | Xiang Lin Xiaohui Wang Fan Xiao Kongyang Ma Lixiong Liu Xiaoqi Wang Dong Xu Fei Wang Xiaofei Shi Dongzhou Liu Yan Zhao Liwei Lu | 2019 | Cellular & Molecular Immunology2019，16，12： | 13 |
| 6 | DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells显示文摘染色质改变为人的初发的细菌房间的 epigenetic reprogramming 是重要的。然而,全面染色质状态还没为人的胎儿的细菌房间(FGC ) 被分析了。这里,我们使用 nucleosome 占有和 methylation 定序方法在人和老鼠在胎儿的细菌房间开发期间在一系列关键时间点分析染色体宽的染色质可接近性和 DNA methylome。我们在人和老鼠 FGC 发现 116 887 和 137 557 个弄空 nucleosome 的区域(NDR ) ,盖住 germline 特定、高度有活力的规章的 genomic 元素的一个大集合,例如 enhancers。而且,我们发现远侧的 NDR 在人的 FGC 为象 NANOG , SOX17 , AP2 和 OCT4 那样的 pluripotency 和细菌房间主人管理者的有约束力的主题明确地被充实,显示在pluripotency相关的基因和细菌之间的精细的规章的平衡的存在在人的 FGC 的房间特定的基因,并且为在 vivo 的细菌房间开发的这些基因的功能的意义。我们的工作为把提供一张全面、高分辨率的路线图染色质状态转变动力学在人和老鼠 FGC 的 epigenomic reprogramming 期间。 | Hongshan Guo Boqiang Hu Liying Yan Jun Yong Yan Wu Yun Gao Fan Guo Yu Hou Xiaoying Fan Ji Dong Xiaoye Wang Xiaohui Zhu Jie Yan Yuan Wei Hongyan Jin Wenxin Zhang Lu Wen FuchouTang Jie Qiao | 2017 | Cell Research2017，27，2： | 9 |
| 7 | The long-term variability of sea surface temperature in the seas east of China in the past 40 a显示文摘The global surface temperature change since the mid-19th century has caused general concern and intensive study.However,long-term changes in the marginal seas,including the seas east of China,are not well understood because long-term observations are sparse and,even when they exist,they are over limited areas.Preliminary results on the long-term variability of sea surface temperature(SST) in summer and winter in the seas east of China during the period of 1957-2001 are reported using the Ocean Science Database of Institute of Oceanology,Chinese Academy of Sciences,the coastal hydrological station in situ and satellite data.The results show well-defined warming trends in the study area.However warming and cooling trends vary from decade to decade,with steady and rapid warming trends after the 1980s and complicated spatial patterns.The distribution of SST variation is intricate and more blurred in the areas far away from the Kuroshio system.Both historical and satellite data sets show significant warming trends after 1985.The warming trends are larger and spread to wider areas in winter than in summer,which means decrease in the seasonal cycle of SST probably linked with recently observed increase of the tropical zooplankton species in the region.Spatial structures of the SST trends are roughly consistent with the circulation pattern especially in winter when the meridional SST gradients are larger,suggesting that a horizontal advection may play an important role in the long-term SST variability in winter. | WANG Fan MENG Qingjia TANG Xiaohui HU Dunxin | 2013 | Acta Oceanologica Sinica2013，32，3： | 9 |
| 8 | Effects of Plant Growth-Promoting Rhizobacteria and N Source on Plant Growth and N and P Uptake by Tomato Grown on Calcareous Soils显示文摘Introducing specific microorganisms into the soil ecological system is an important strategy for improving nutrient use efficiency.Two pot experiments were conducted in the greenhouse from December 3, 2012 to January 25, 2013(Experiment 1) and March 11 to April 23, 2013(Experiment 2) to evaluate the effect of nitrogen(N) source and inoculation with plant growth-promoting rhizobacteria(PGPR) on plant growth and N and phosphorus(P) uptake in tomato(Lycopersicon esculentum Mill.) grown on calcareous soils from South Florida, USA.Treatments included urea, controlled release urea(a controlled release fertilizer, CRF) each at low and high N rates and with or without inoculation of PGPR.A mixture of PGPR strains Bacillus amyloliquefaciens IN937 a and Bacillus pumilus T4 was applied to the soil during growing periods of tomato.Treatments with PGPR inoculation increased plant height compared to treatments without PGPR in both experiments.Inoculation with PGPR increased shoot dry weight and shoot N uptake for the same N rate and N source.In both experiments, only at high N rate, CRF and urea treatments with PGPR had significantly(P < 0.05)greater shoot biomass than those without PGPR.Only at high N rate, CRF treatment with PGPR significantly increased shoot N uptake by 39.0% and 10.3% compared to that without PGPR in Experiments 1 and 2, respectively.Meanwhile, presence of PGPR in the soil increased shoot P uptake for all treatments in Experiment 1 and for most treatments in Experiment 2.In Experiment 1, only at low N rate, CRF treatment with PGPR significantly increased shoot P uptake compared with that without PGPR.In Experiment2, a significant increase in shoot P uptake by inoculation of PGPR was only observed in CRF treatment at high N rate.Results from this study indicate that inoculation with PGPR may increase plant growth and N and P uptake by tomato grown on calcareous soils.However, the effect of PGPR varied and was influenced by many factors such as N source, N rate, and soil fertility.Further investigations are warranted to confirm the effect of PGPR under different soil conditions. | Xiaohui FAN Shouan ZHANG Xiaodan MO Yuncong LI Yuqing FU Zhiguang LIU | 2017 | Pedosphere2017，27，6： | 8 |
| 9 | Tissue resident regulatory T cells: novel therapeutic targets for human disease显示文摘 | Xiaohui Zhou Jiayou Tang Hao Cao Huimin Fan Bin Li | 2015 | Cellular & Molecular Immunology2015，12，5： | 7 |
| 10 | Proteasome inhibition suppresses Th17 cell generation and ameliorates autoimmune development in experimental Sjögren’s syndrome显示文摘Immunoproteasome activation in immune cells is involved in the modulation of immune responses.Increasing evidence indicates that proteasome inhibitors show beneficial effects in treating autoimmune diseases,but it remains unclear whether proteasome inhibition is an effective approach for suppressing autoimmune development in Sjögren’s syndrome(SS).Our previous work has demonstrated a critical role for Th17 cells in the development of experimental SS(ESS)in mice.In this study,we detected high levels of low-molecular-weight protein 7(LMP7),a key subunit of the immunoproteasome,in Th17 cells from ESS mice.Moreover,treatment with bortezomib(BTZ),a proteasome inhibitor,markedly suppressed Th17 differentiation in both murine and human naive T cells in culture.Furthermore,ESS mice treated with BTZ displayed significantly higher saliva flow rates and a reduction in tissue destruction in the salivary glands compared with vehicle-treated ESS mice.Notably,BTZ-treated ESS mice showed markedly decreased Th17 cells,germinal center B cells and plasma cells in the peripheral lymphoid organs.In addition,adoptively transferred wild type naive CD4+T cells rapidly differentiated into Th17 cells and induced salivary dysfunction in IL-17-deficient mice immunized for ESS induction.However,BTZ treatment profoundly suppressed the donor T-cell-derived Th17 response and ameliorated the reduction in salivary secretion in IL-17-deficient recipient mice.Taken together,our findings demonstrate that proteasome inhibition can effectively ameliorate ESS by suppressing the Th17 response,which may contribute to the development of a novel therapeutic strategy for the treatment of SS. | Fan Xiao Xiang Lin Jie Tian Xiaohui Wang Qian Chen Ke Rui Jie Ma Shengjun Wang Qingwen Wang Xiaoqi Wang Dongzhou Liu Lingyun Sun Liwei Lu | 2017 | Cellular & Molecular Immunology2017，14，11： | 6 |
| 11 | Histopathological Features and Viral Antigen Distribution in the Lung of Fatal Patients with Enterovirus 71 Infection显示文摘Dear Editor,Enterovirus 71(EV71)infection causes hand-foot-andmouth disease(HFMD)in infants and children.Patients with HFMD usually have good prognosis;however,in some extreme cases the infection can be accompanied by central nervous system diseases,eventually leading to cardiorespiratory failure,and even death.Currently。 | Zhenli Liang Hongbo Pan Xijing Wang Yinchuan Zhu Yiwu Dang Xiaohui Fan Lingxi Gao Zengfeng Zhang | 2018 | Virologica Sinica2018，33，3： | 4 |
| 12 | An ultra-robust fingerprinting method for quality assessment of traditional Chinese medicine using multiple reaction monitoring mass spectrometry显示文摘Chromatographic fingerprinting has been perceived as an essential tool for assessing quality and chemical equivalence of traditional Chinese medicine.However,this pattern-oriented approach still has some weak points in terms of chemical coverage and robustness.In this work,we proposed a multiple reaction monitoring(MRM)-based fingerprinting method in which approximately 100 constituents were simultaneously detected for quality assessment.The derivative MRM approach was employed to rapidly design MRM transitions independent of chemical standards,based on which the large-scale fingerprinting method was efficiently established.This approach was exemplified on QiShenYiQi Pill(QSYQ),a traditional Chinese medicine-derived drug product,and its robustness was systematically evaluated by four indices:clustering analysis by principal component analysis,similarity analysis by the congruence coefficient,the number of separated peaks,and the peak area proportion of separated peaks.Compared with conventional ultraviolet-based fingerprints,the MRM fingerprints provided not only better discriminatory capacity for the tested normal/abnormal QSYQ samples,but also higher robustness under different chromatographic conditions(i.e.,flow rate,apparent pH,column temperature,and column).The result also showed for such large-scale fingerprints including a large number of peaks,the angle cosine measure after min-max normalization was more suitable for setting a decision criterion than the unnormalized algorithm.This proof-of-concept application gives evidence that combining MRM technique with proper similarity analysis metrices can provide a highly sensitive,robust and comprehensive analytical approach for quality assessment of traditional Chinese medicine. | Zhenhao Li Xiaohui Zhang Jie Liao Xiaohui Fan Yiyu Cheng | 2021 | Journal of Pharmaceutical Analysis2021，11，1： | 4 |
| 13 | The effect of extrusion conditions on the properties and textures of AZ31B alloy显示文摘The effect of extrusion conditions on the tensile properties and texture of AZ31B alloy has been investigated by means of optical microscopy(OM),scanning electron microscopy(SEM),electron backscattered diffraction(EBSD)and tensile tests.It is found that the ultimate tensile strength(UTS),the yield strength(YS)and elongation(EN)of the extruded AZ31B alloy are more significantly influenced by extrusion velocities in contrast with temperature.Although the extrusion conditions are different,the {1102}<0101> texture is the chief texture in the AZ31B after extrusion.Moreover,the extrusion textures become scattered with increasing the temperatures at the same extrusion velocity.As the extrusion velocity is raised at the same temperature,the orientation density of textures increases and the separated textures become relatively concentrated.This leads to the changes of tensile properties at different extrusion conditions.©2017 Production and hosting by Elsevier B.V.on behalf of Chongqing University. | Qiang Liu Xiao Zhou Haitao Zhou Xiaohui Fan Keming Liu | 2017 | Journal of Magnesium and Alloys2017，5，2： | 4 |
| 14 | Changes in wind activity from 1957 to 2011 and their possible influence on aeolian desertification in northern China显示文摘Wind activity is proved to have significant impacts on aeolian desertification. Clarifying the fluctuations and change trend of wind velocity is important for understanding their influence on aeolian desertification. In this study, we used a dataset of wind velocities collected from 93 meteorological stations across northern China from 1957 to 2011 to analyze the changes in wind activity during this period. We tested the monotonic and step(abrupt) trends for annual and seasonal data of mean wind velocity by using Mann-Kendall and Mann-Whitney tests, respectively. The results indicated that the annual mean wind velocity decreased by 0.83 m/s from 1957 to 2011. The decreasing trends were also significant(P<0.01) for each season. The magnitude of the decrease was smallest in the east of northern China and largest in the west of northern China, and the most remarkable decrease occurred in the northwest of northern China. Abrupt decreases in annual and seasonal mean wind velocities occurred in the mid-1980 s, which was consistent with the changes in aeolian desertification since the mid-1980 s in northern China. As revealed by our study, although both modern aeolian desertification and ecosystem rehabilitation are affected by human activities to some extent, they are also likely to be strongly controlled by climate change, especially by wind activity. | LI Jinchang LIU Haixia SU Zhizhu FAN Xiaohui | 2015 | Journal of Arid Land2015，7，6： | 4 |
| 15 | Mechanical Properties of Resistance Spot Welded Components of High Strength Austenitic Stainless Steel显示文摘Resistance multi-spot welding(MSW)in column,triangle and tetragonal symmetry arrangements was prepared using cold-rolled 301L high-strength sheets,and their static and fatigue properties were investigated.The effects of spot welds on the fracture strengths and fatigue limits were discussed.The results show that the static strengths can be estimated using an inherent linear relationship formula of the load versus the welding area.It was based on the 28%–33%volume fraction ofα′martensite induced at the interfacial spot weld fracture because of the failure deformation.The fatigue limits of the MSW nonlinearly increase with the number of spot welds.The arrangement of spot welds in the MSW significantly affects the average fatigue limit of each spot weld;its 26%maximum reduction occurred in the triangle,and the interaction stress between spot welds led to its 18%reduction in the tetragonal symmetry.The calculated fatigue stress of all MSW loadings with their mean fatigue limits was 230–270 MPa. | Wei Liu Hailong Fan Xiangzhong Guo Zhihong Huang Xiaohui Han | 2016 | Journal of Materials Science & Technology2016，32，6： | 4 |
| 16 | New avenues for systematically inferring cellcell communication:through single-cell transcriptomics data显示文摘For multicellular organisms,cell-cell communication is essential to numerous biological processes.Drawing upon the latest development of single-cell RNA-sequencing(scRNA-seq),high-resolution transcriptomic data have deepened our understanding of cellular phenotype heterogeneity and composition of complex tissues,which enables systematic cell-cell communication studies at a single-cell level.We first summarize a common workflow of cell-cell communication study using scRNA-seq data,which often includes data preparation,construction of communication networks,and result validation.Two common strategies taken to uncover cell-cell communications are reviewed,e.g.,physically vicinal structure-based and ligand-receptor interaction-based one.To conclude,challenges and current applications of cell-cell communication studies at a single-cell resolution are discussed in details and future perspectives are proposed. | Xin Shao Xiaoyan Lu Jie Liao Huajun Chen Xiaohui Fan | 2020 | Protein & Cell2020，11，12： | 4 |
| 17 | The epigallocatechin gallate derivative Y_6 reverses drug resistance mediated by the ABCB1 transporter both in vitro and in vivo显示文摘Previously, we reported that Y_6, a new epigallocatechin gallate derivative, is efficacious in reversing doxorubicin(DOX)–mediated resistance in hepatocellular carcinoma BEL-7404/DOX cells. In this study, we evaluated the efficacy of Y_6 in reversing drug resistance both in vitro and in vivo by determining its effect on the adenosine triphosphate-binding cassette protein B1 transporter(ABCB1 or P-glycoprotein, P-gp). Our results showed that Y_6 significantly sensitized cells overexpressing the ABCB1 transporter to anticancer drugs that are ABCB1 substrates. Y_6 significantly stimulated the adenosine triphosphatase activity of ABCB1. Furthermore, Y_6 exhibited a higher docking score as compared with epigallocatechin gallate inside the transmembrane domain of ABCB1. In addition, in the nude mousetumor xenograft model, Y_6(110 mg/kg, intragastric administration), in combination with doxorubicin(2 mg/kg, intraperitoneal injection), significantly inhibited the growth of BEL-7404/DOX cell xenograft tumors, compared to equivalent epigallocatechin gallate. In conclusion, Y_6 significantly reversed ABCB1-mediated multidrug resistance and its mechanisms of action may result from its competitive inhibition of the ABCB1 drug efflux function. | Yan Wen Ruiqiang Zhao Pranav Gupta Yingfang Fan Yunkai Zhang Zhenguang Huang Xiaohui Li Yuangang Su Lijuan Liao Yu-An Xie Donghua Yang Zhe-Sheng Chen Gang Liang | 2019 | Acta Pharmaceutica Sinica B2019，9，2： | 3 |
| 18 | Neuroprotective effects of Ginkgo biloba dropping pills in Parkinson's disease显示文摘Parkinson's disease(PD) is the second most common neurodegenerative disease in the world;however,it lacks effective and safe treatments. Ginkgo biloba dropping pill(GBDP), a unique Chinese G. biloba leaf extract preparation, exhibits antioxidant and neuroprotective effects and has a potential as an alternative therapy for PD. Thus, the aims of this study were to evaluate the effects of GBDP in in vitro and in vivo PD models and to compare the chemical constituents and pharmacological activities of GBDP and the G. biloba extract EGb 761. Using liquid chromatography tandem-mass spectrometry, 46 GBDP constituents were identified. Principal component analysis identified differences in the chemical profiles of GBDP and EGb 761. A quantitative analysis of 12 constituents showed that GBDP had higher levels of several flavonoids and terpene trilactones than EGb 761, whereas EGb 761 had higher levels of organic acids.Moreover, we found that GBDP prevented 6-hydroxydopamine-induced dopaminergic neuron loss in zebrafish and improved cognitive impairment and neuronal damage in methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mice. Although similar effects were observed after EGb 761 treatment,the neuroprotective effects were greater after GBDP treatment on several endpoints. In addition, in vitro results suggested that the Akt/GSK3β pathway may be involved in the neuroprotective effects of GBDP.These findings demonstrated that GBDP have potential neuroprotective effects in the treatment of PD. | Dingyi Yu Pengli Zhang Junying Li Ting Liu Yaodan Zhang Qingqing Wang Jianbing Zhang Xiaoyan Lu Xiaohui Fan | 2021 | Journal of Pharmaceutical Analysis2021，11，2： | 3 |
| 19 | Establishment of a Spleen Cell Line from Large Yellow Croaker Pseudosciaena crocea and its Primitive Application in Foreign Gene Transfection显示文摘A large yellow croaker,Pseudosciaena crocea,spleen(LYCS)cell line was established and the feasibility of using it for foreign gene transfection was evaluaed in this study.Primary culture of LYCS cells was initiated from spleen tissue pieces,which were cultured at 25℃ in Dulbecco's modiced Eagle medium/F12 medium(DMEM/F12,1:1)(pH7.2),supplemented with 20% fetal bovine serum,carboxymethyl chitosan,chondroitin sulfate,basic fibroblast growth factor(bFGF)and insulin-like growth factor-I(IGF-I).The cultured LYCS cells,in fibroblast shape,proliferated to 100% confluency 20 days later.Chromosome analyses indicated that the LYCS cells exhibited chromosomal aneuploidy with a modal chromosome number of 48 which displayed the normal diploid karyotype of P.crocea(6m+6sm+36t,NF=60).A LYCS cell line,with a population doubling time of 48.7 h at passage 60,has been established and subcultured to passage 70.Transgenic feasibility test demonstrated that positive green fluorescence protein(GFP)expression was observed in LYCS cells after pcDNA3.1-GFP plasmid transfection.In conclusion,a continuous foreign gene trans-fection feasible LYCS cell line has been established successfully.The cell line might serve as a valuable tool for studies of transgenic breeding and has potential applications for different kinds of cytotechnological studies. | SUN Ai FAN Tingjun YANG Xiuxia XU Bin XU Xiaohui GUO Xueyang | 2011 | Journal of Ocean University of China2011，10，4： | 3 |
| 20 | Spatial distribution characteristics of bacterial community structure and gene abundance in sediments of the Bohai Sea显示文摘This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants. | Quanrui Chen Jingfeng Fan Jie Su Hongxia Ming Zhihao Sun Mengfei Li Xiaohui Zhao Yantao Wang Yingxue Zhang Huizhen Zhang Yuan Jin Xiaowan Ma Bin Wang | 2020 | Acta Oceanologica Sinica2020，39，2： | 2 |
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