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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Antigen-presenting effects of effector memory Vγ9Vδ2 T cells in rheumatoid arthritis显示文摘 | Chaoying Hu Liu Qian Yi Miao Qiuyu Huang Ping Miao Ping Wang Qiwen Yu Hong Nie Jiying Zhang Dongyi He Rong Xu Xuehua Chen Bingya Liu Dongqing Zhang | 2012 | Cellular & Molecular Immunology2012，9，3： | 18 |
| 3 | Molecular Typing of Leptospira interrogans Strains Isolated from Rattus tanezumi in Guizhou Province,Southwest of China显示文摘Objective To identify and type three leptospires isolated from Rattus tanezumi in Guizhou Province by using three molecular techniques (PFGE, MLVA, and MLST), reveal the molecular characteristic of causative agents of local leptospirosis and evaluate these three molecular methods based on their detection resolution and efficiency. Methods Three Leptospira strains were isolated from the kidney of Rattus tanezumi and cultured with EMJH medium. PFGE, MLVA, and MLST assays were applied to type the three strains isolated from Rattus tanezumi in Guizhou Province. Results PFGE, MLVA, and MLST typing showed that the three leptospiral isolates matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai. The findings of the genotyping methods were consistent. MLVA and MLST defined genotypes, whereas PFGE allowed the recognition of additional subgroups within the genotypes, and the findings of molecular typing were also consistent with those of traditional techniques. Conclusion Three leptospiral isolates from Guizhou Province matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai, and PFGE, MLVA, and MLST, as reliable molecular techniques for identifying and typing of Leptospira interrogans, would contribute to the active surveillance, outbreak investigation and source tracking for leptospirosis in Guizhou Province. | LI Shi Jun ZHANG Cui Cai LI Xiu Wen TIAN Ke Cheng TANG Guang Peng WANG Ding Ming LIU Ying NIE Yi Xin JIANG Xiu Gao | 2012 | Biomedical and Environmental Sciences2012，25，5： | 12 |
| 4 | Effects of Film-Degraded Rice Controlled Release Fertilizer on the Nitrogen Uptake and Yield of the Hybrid Rice显示文摘Field experiment was carried out to nitrogen release characteristics of rice controlled release fertilizer (RCRF) coated with natural and half natural high molecular materials, and to thereof effects on the nitrogen uptake and yield of early and late hybrid rice from 1999 to 2000, with urea as control.In experimental fields regardless of early and late seasons, the amount of N in the plants increased exponentially at the early growth stage (y=abx) and nonlinearly at the middle and late growth stages (y=a+bx+cx2).Rice controlled release fertilizer lengthened the exponential phase and increased the parameter -b/c during the nonlinear phase. Although the N amount of plants was less in rice controlled release fertilizer plots than in urea plots within 20 days after transplanting, at the heading and maturity stages, the N amount of plants in rice controlled release fertilizer plots was above two times greater than in the urea plots due to the higher N recovery, and the high absorption density of N in the nonlinear phase. Tillers pattern and achievement of the maximum tillering stage varied with fertilizer types. The maximum tillering stage in the urea plots occurred a week earlier than in the rice controlled release fertilizer plots. Yield sink size and potential sink size of the plants reflected the N absorption pattern and the amount of N in the plants. The yields of early and late rice with 90 kg N ha-1of rice controlled release fertilizer were respectively increased by 832.7 kg ha-1 and 412.8 kg ha-1 than those with 90 kg N ha-1 of urea, almost equivalent to the yield of early and late hybrid rice with 180 kg N ha-1 of urea. | Zheng Shengxian, Nie Jun, Xiao Jian, Xiong Jinying, and Yi Guoying Hunan Soil and Fertilizer Institute, Changsha 410125, China | 2002 | Hunan Agricultural Science & Technology Newsletter2002，3，2： | 12 |
| 5 | Fabrication,microstructures,and properties of copper matrix composites reinforced by molybdenum-coated carbon nanotubes显示文摘Multiwalled carbon nanotubes(CNTs) were coated by a molybdenum layer using carbonyl thermal decomposition process with a precursor of molybdenum hexacarbonyl.The Mo-coated CNTs(Mo-CNTs) were added into copper powders to fabricate Mo-CNT/Cu composites by means of mechanical milling followed by spark plasma sintering.The Mo-CNTs were uniform dispersion in the Cu matrix when their contents were 2.5 vol.%-7.5 vol.%,while some Mo-CNT clusters were clearly observed at additions of 10.0 vol.%-15.0 vol.% Mo-CNTs in the mixture.The mechanical,electrical,and thermal properties of the Mo-CNT/Cu composites were characterized,and the results showed that the tensile strength and hardness were 2.0 and 2.2 times higher than those of CNT-free specimens,respectively.Moreover,the Mo-CNT/Cu composites exhibited an enhanced thermal conductivity but inferior electrical conductivity compared with sintered pure Cu.The uncoated CNT/Cu composites were fabricated by the similar processes,and the measured tensile strength,hardness,thermal conductivity,and electrical conductivity of the CNT/Cu composites were lower than those of the Mo-CNT/Cu composites. | NIE Junhui JIA Chengchang JIA Xian ZHANG Yafeng SHI Na LI Yi | 2011 | Rare Metals2011，30，4： | 12 |
| 6 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 7 | Controlled Teleportation Using Four-Particle Cluster State显示文摘A controlled teleportation scheme is presented.In this scheme,quantum information of a single-qubit stateor an entangled two-qubits state is transmitted from a sender (Alice) to a receiver (Charlie) via a four-particle clusterstate under the control of the supervisor (Bob).The feature of this scheme is that the teleportation between the senderand the receiver depends on the control of the supervisor. | LI Song-Song NIE Yi-You HONG Zhi-Hui YI Xiao-Jie HUANG Yi-Bin | 2008 | Communications in Theoretical Physics2008，50，9： | 11 |
| 8 | Deep learning applied to two-dimensional color Doppler flow imaging ultrasound images significantly improves diagnostic performance in the classification of breast masses:a multicenter study显示文摘Background:The current deep learning diagnosis of breast masses is mainly reflected by the diagnosis of benign and malignant lesions.In China,breast masses are divided into four categories according to the treatment method:inflammatory masses,adenosis,benign tumors,and malignant tumors.These categorizations are important for guiding clinical treatment.In this study,we aimed to develop a convolutional neural network(CNN)for classification of these four breast mass types using ultrasound(US)images.Methods:Taking breast biopsy or pathological examinations as the reference standard,CNNs were used to establish models for the four-way classification of 3623 breast cancer patients from 13 centers.The patients were randomly divided into training and test groups(n=1810 vs.n=1813).Separate models were created for two-dimensional(2D)images only,2D and color Doppler flow imaging(2D-CDFI),and 2D-CDFI and pulsed wave Doppler(2D-CDFI-PW)images.The performance of these three models was compared using sensitivity,specificity,area under receiver operating characteristic curve(AUC),positive(PPV)and negative predictive values(NPV),positive(LR+)and negative likelihood ratios(LR-),and the performance of the 2D model was further compared between masses of different sizes with above statistical indicators,between images from different hospitals with AUC,and with the performance of 37 radiologists.Results:The accuracies of the 2D,2D-CDFI,and 2D-CDFI-PW models on the test set were 87.9%,89.2%,and 88.7%,respectively.The AUCs for classification of benign tumors,malignant tumors,inflammatory masses,and adenosis were 0.90,0.91,0.90,and 0.89,respectively(95%confidence intervals[CIs],0.87-0.91,0.89-0.92,0.87-0.91,and 0.86-0.90).The 2D-CDFI model showed better accuracy(89.2%)on the test set than the 2D(87.9%)and 2D-CDFI-PW(88.7%)models.The 2D model showed accuracy of 81.7%on breast masses≤1 cm and 82.3%on breast masses>1 cm;there was a significant difference between the two groups(P<0.001).The accuracy of the CNN classifications for the test set(89.2%)was significantly higher than that of all the radiologists(30%).Conclusions:The CNN may have high accuracy for classification of US images of breast masses and perform significantly better than human radiologists.Trial registration:Chictr.org,ChiCTR1900021375;http://www.chictr.org.cn/showproj.aspx?proj=33139. | Teng-Fei Yu Wen He Cong-Gui Gan Ming-Chang Zhao Qiang Zhu Wei Zhang Hui Wang Yu-Kun Luo Fang Nie Li-Jun Yuan Yong Wang Yan-Li Guo Jian-Jun Yuan Li-Tao Ruan Yi-Cheng Wang Rui-Fang Zhang Hong-Xia Zhang Bin Ning Hai-Man Song Shuai Zheng Yi Li Yang Guang | 2021 | Chinese Medical Journal2021，，4： | 11 |
| 9 | Attention-based encoder-decoder model for answer selection in question answering显示文摘问答技术的重要挑战之一就是解决问题与答案之间的语义空白。机器翻译模型已经被证明能有效的提升解决问题与答案之间的语义空白。本文提出了一种基于注意机制的深度神经网络模型来解决问答系统中的答案选择任务。该模型采用了基于双向长短时记忆(Long short-term memory,LSTM)的编码解码模型,编码解码模型是一个被证明再机器翻译领域取得了突出的成绩。我们还在模型中应用了注意力机制来提升模型的效果。本文在一个公开数据集上验证了实验的有效性,同时通过结合该模型显著提高了问答系统的性能在TREC 2015 liveQA的任务中。 | Yuan-ping NIE Yi HAN Jiu-ming HUANG Bo JIAO Ai-ping LI | 2017 | Frontiers of Information Technology & Electronic Engineering2017，18，4： | 11 |
| 10 | Involvement of ayu NOD2 in NF-κB and MAPK signaling pathways: Insights into functional conservation of NOD2 in antibacterial innate immunity显示文摘Nucleotide oligomerization domain 2(NOD2) is a major cytoplasmic sensor for pathogens and is critical for the clearance of cytosolic bacteria in mammals.However, studies regarding NOD2, especially the initiated signaling pathways, are scarce in teleost species. In this study, we identified a NOD2 molecule(PaNOD2) from ayu(Plecoglossus altivelis).Bioinformatics analysis showed the structure of NOD2 to be highly conserved during vertebrate evolution. Dual-luciferase reporter assays examined the activation of NF-κB signaling and Western blotting analysis detected the phosphorylation of three MAP kinases(p-38, Erk1/2, and JNK1/2).Functional study revealed that, like its mammalian counterparts, PaNOD2 was the receptor of the bacterial cell wall component muramyl dipeptide(MDP), and the leucine-rich repeat motif was responsible for the recognition and binding of Pa NOD2 with the ligand. Overexpression of PaNOD2 activated the NF-κB signaling pathway, leading to the upregulation of inflammatory cytokines, including TNF-α and IL-1β in HEK293 T cells and ayu head kidney-derived monocytes/macrophages(MO/MΦ).Particularly, we found that PaNOD2 activated the MAPK signaling pathways, as indicated by the increased phosphorylation of p-38, Erk1/2, and JNK1/2, which have not been characterized in any teleost species previously. Our findings proved that the NOD2 molecule and initiated pathways are conserved between mammals and ayu. Therefore, ayu could be used as an animal model to investigate NOD2-based diseases and therapeutic applications. | Yi Ren Shui-Fang Liu Li Nie Shi-Yu Cai Jiong Chen | 2019 | Zoological Research2019，40，2： | 10 |
| 11 | Tracking Performance of Electric Power Steering System Based on the Mixed H_2/H_∞ Strategy显示文摘The tracking performance of motor current is an important factor that affects the assistance torque of electric power steering (EPS) system. Bad tracking performance will cause assistant torque delay, and make road feeling bad, and is influenced by the input steering torque and system measuring noise. However the existing methods have some shortages on system's robust dynamic performance and robust stability. The mixed H2/H∞ strategy for recirculating ball-type EPS system in a pure electric bus is proposed, and vehicle dynamic model of the system is established. Due to the existence of system model uncertainty, disturbance signals, sensor noises and the demand of system dynamic performance, the indexes of robust performance and road feeling for drivers are defined as the appraisal control objectives. The H∞ method is introduced to design the H∞ controller, and the H2 method is applied to optimize the H∞ controller, thus the mixed H2/H∞ controller is designed. The response of EPS system to the motor current command with amplitude of 20 A, the road disturbance with amplitude of 500 N and the sensor random noise with the amplitude of 1 A is simulated. The simulation results show that the recirculating ball-type EPS system with the mixed H2/H∞ controller can attenuate the random noises and disturbances and track the boost curve well, so the mixed H2/H∞ controller can improve the system's robust performance and dynamic performance. For the purpose of verifying the performance of the designed control strategy, the motor current tracking performance ground tests are conducted with step response input of the steering wheel, double-lane steering test and lemniscate steering test, respectively. The tests show that the mixed H2/H∞ controller for the recirculating ball-type EPS system of pure electric bus is feasible. The designed controller can solve the robust performance and robust stability of the system, thus improve the tracking performance of the EPS system and provide satisfied road feeling for the drivers. | ZHAO Wanzhong SHI Guobiao LIN Yi NIE Hong | 2011 | Chinese Journal of Mechanical Engineering2011，24，4： | 10 |
| 12 | DDIT4 promotes gastric cancer proliferation and tumorigenesis through the p53 and MAPK pathways显示文摘Background:Gastric cancer(GC)is one of the most common malignancies worldwide,particularly in China.DNA damage-inducible transcript 4(DDIT4)is a mammalian target of rapamycin inhibitor and is induced by various cellular stresses;however,its critical role in GC remains poorly understood.The present study aimed to investigate the poten-tial relationship and the underlying mechanism between DDIT4 and GC development.Methods:We used western blotting,real-time polymerase chain reaction,and immunohistochemical or immunoflu-orescence to determine DDIT4 expression in GC cells and tissues.High-content screening,cell counting kit-8 assays,colony formation,and in vivo tumorigenesis assays were performed to evaluate cell proliferation.Flow cytometry was used to investigate cell apoptosis and cell cycle distribution.Results:DDIT4 was upregulated in GC cells and tissue.Furthermore,downregulating DDIT4 in GC cells inhibited proliferation both in vitro and in vivo and increased 5-fluorouracil-induced apoptosis and cell cycle arrest.In contrast,ectopic expression of DDIT4 in normal gastric epithelial cells promoted proliferation and attenuated chemosensitivity.Further analysis indicated that the mitogen-activated protein kinase and p53 signaling pathways were involved in the suppression of proliferation,and increased chemosensitivity upon DDIT4 downregulation.Conclusion:DDIT4 promotes GC proliferation and tumorigenesis,providing new insights into the role of DDIT4 in the tumorigenesis of human GC. | Feng Du Lina Sun Yi Chu Tingyu Li Chao Lei Xin Wang Mingzuo Jiang Yali Min Yuanyuan Lu Xiaodi Zhao Yongzhan Nie Daiming Fan | 2018 | Cancer Communications2018，38，1： | 10 |
| 13 | Risk factors of intra-abdominal bacterial infection after liver transplantation in patients with hepatocellular carcinoma显示文摘Objective: To explore the risk factors of intra-abdominal bacterial infection(IAI) after liver transplantation(LT) in patients with hepatocellular carcinoma(HCC). Methods: A series of 82 HCC patients who received LT surgeries in our department between March 2004 and April 2010 was recruited in this study. Then we collected and analyzed the clinical data retrospectively. Statistical analysis system(SPSS) software was adopted to perform statistical analysis. Chi-square test, t-test and Wilcoxon rank sum test were used to analyze the clinical data and compute the significance of the incidences of early-stage IAI after LT for HCC patients. Binary logistic regression was performed to screen out the risk factors, and multiple logistic regression analyses were performed to compute the independent risk factors. Results: A series of 13 patients(13/82, 15.9%) had postoperative IAI. The independent risk factors of postoperative intra-abdominal bacterial infections after LT for HCC patients were preoperative anemia [Hemoglobin(HGB) <90 g/L] and postoperative abdominal hemorrhage(72 hours >400 mL), with the odds ratios at 8.121(95% CI, 1.417 to 46.550, P=0.019) and 5.911(95% CI, 1.112 to 31.432, P=0.037). Conclusions: Postoperative IAI after LT in patients with HCC was a common complication. Preoperative moderate to severe anemia, as well as postoperative intra-abdominal hemorrhage more than 400 mL within the first 72 hours might independently indicate high risk of IAI for these patients. | Kai Nie Rongzheng Ran Weifeng Tan Bin Yi Xiangji Luo Yong Yu Xiaoqing Jiang | 2014 | Chinese Journal of Cancer Research2014，26，3： | 10 |
| 14 | Investigation of dielectric barrier discharge plasma flow control显示文摘Effects of plasma flow control are researched on the basis of plasma exciting flow experiments and numerical simulations. Turbulent model is more effective than laminar model in plasma numerical simulation as results showed. Both plasma exciting effects of acceleration and flow separation suppression are investigated through experiments carried on the flat plate and the compressor cascades. The results demonstrate that boundary layer characteristic is modified by plasma exciting. Distributions of total pressure and velocity in the wake are improved notably for 20 m/s coming velocity and the effect of plasma can still be observed while velocity is increased to 50 m/s. For low velocity flow, plasma exciting is effective in flow separation suppression. | NIE ChaoQun LI Gang ZHU JunQiang ZHANG Yi LI YuTong | 2008 | Science China(Technological Sciences)2008，51，7： | 9 |
| 15 | Aluminum matrix composites reinforced by molybdenum-coated carbon nanotubes显示文摘To extend the application of carbon nanotubes (CNTs) and explore novel aluminum matrix composites,CNTs were coated by molybdenum layers using metal organic chemical vapor deposition,and then Mo-coated CNT (Mo-CNT)/Al composites were prepared by the combination processes of powder mixing and spark plasma sintering.The influences of powder mixing and Mo-CNT content on the mechanical properties and electrical conductivity of the composites were investigated.The results show that magnetic stirring is better than mechanical milling for mixing the Mo-CNTs and Al powders.The electrical conductivity of the composites decreases with increasing Mo-CNT content.When the Mo-CNT content is 0.5wt%,the tensile strength and hardness of Mo-CNT/Al reach their maximum values.The tensile strength of 0.5wt% Mo-CNT/Al increases by 29.9%,while the electrical conductivity only decreases by 7.1%,relative to sintered pure Al.The phase analysis of Mo-CNT/Al composites reveals that there is no formation of Al carbide in the composites. | Jun-hui Nie Cheng-chang Jia Na Shi Ya-feng Zhang Yi Li XianJia | 2011 | International Journal of Minerals,Metallurgy and Materials2011，18，6： | 9 |
| 16 | Effect of basic fibroblast growth factor on the proliferation, migration and phenotypic modulation of airway smooth muscle cells显示文摘背景:增长,细胞移行和航线平滑肌房间(ASMC ) 的表型的调整是航线在气喘改变的重要特征。在肺调整 ASMC 增长,移植和表型的调整的精确细胞、分子的机制仍然保持未知。基本成纤维细胞生长因素(bFGF ) ,为许多房间的一个高度特定的趋化性、致有丝分裂的因素打字,看起来涉及航线改变的发展。我们的学习估计了 bFGF 是否直接刺激增长,迁居和 ASMC 的表型的调整。方法:汇合并且生长逮捕了人的 ASMC 与人的 recombinant FGF 被对待。增长被 BrdU 加入并且房间数测量。移植用 Boyden 房间仪器被检验。平滑肌(sm ) 的表情 -alpha-actin 和 sm 肌浆球蛋白重链(MHC ) 对碘氧基苯甲醚 1 被 RT-PCR 和西方的污点分析决定。结果:当增加了 ASMC 时, hrbFGF (10 ng/ml ) 关于控制作为与在 ASMC 移植的控制和重要增加相比由 ASMC 在 BrdU 举起和房间数字导致了重要增加,这被发现。在与 hrbFGF 被刺激的 ASMC 的 sm-alpha-actin 和 sm-MHC 的 mRNA 和蛋白质表情关于控制减少了。结论:然而,看来, bFGF 能直接刺激 ASMC 的增长和移植房间的有收缩力的显型的表达式减少了。 | ZOU Hui NIE Xiu-hong ZHANG Yi HU Mu ZHANG Yu Alex | 2008 | Chinese Medical Journal2008，，5： | 9 |
| 17 | Immobilization of functional nano-objects in living engineered bacterial biofilms for catalytic applications显示文摘Nanoscale objects feature very large surface-area-to-volume ratios and are now understood as powerful tools for catalysis,but their nature as nanomaterials brings challenges including toxicity and nanomaterial pollution.Immobilization is considered a feasible strategy for addressing these limitations.Here,as a proof-of-concept for the immobilization of nanoscale catalysts in the extracellular matrix of bacterial biofilms,we genetically engineered amyloid monomers of the Escherichia coli curli nanofiber system that are secreted and can self-assemble and anchor nano-objects in a spatially precise manner.We demonstrated three scalable,tunable and reusable catalysis systems:biofilm-anchored gold nanoparticles to reduce nitro aromatic compounds such as the pollutant p-nitrophenol,biofilm-anchored hybrid Cd0.9Zn0.1S quantum dots and gold nanoparticles to degrade organic dyes and biofilm-anchored CdSeS@ZnS quantum dots in a semi-artificial photosynthesis system for hydrogen production.Our work demonstrates how the ability of biofilms to grow in scalable and complex spatial arrangements can be exploited for catalytic applications and clearly illustrates the design utility of segregating high-energy nano-objects from injury-prone cellular components by engineering anchoring points in an extracellular matrix. | Xinyu Wang Jiahua Pu Yi Liu Fang Ba Mengkui Cui Ke Li Yu Xie Yan Nie Qixi Mi Tao Li Lingli Liu Manzhou Zhu Chao Zhong | 2019 | National Science Review2019，6，5： | 9 |
| 18 | Harnessing the collective properties of nanoparticle ensembles for cancer theranostics显示文摘单个无机的 nanoparticles (NP ) 广泛地在药交货,癌症成像和治疗的地里被使用了。仍然有许多障碍,为这些应用程序限制单个 NP 的性能。高度订的 NP 整体的利用打开一扇门解决这些问题,由于他们的新或先进的集体性质。装配 NP 在单个基于 NP 的系统上显示出几个优点,例如指向的改进房间成为主观和肿瘤,提高的 multimodality 成像能力,优异联合治疗从 synergistic 产生完成,从由进原来的小 NP 积木的集会的降级的整个身体的可能的完全的清理等等。在这评论,我们讨论由拿 plasmonic 为癌症成像和治疗利用装配 NP 整体的潜力 Au NP 的小囊的集会作为一个例子。我们首先总结最近的开发在 plasmonic 自己组装从 amphiphilic 的 NP 的小囊的结构拴住聚合物的 NP 积木。我们进一步为癌症成像考察 NP 的 plasmonic 泡的利用(例如多光子导致了光, photothermal,和 photoacoustic 成像) ,并且癌症治疗(例如, photothermal 治疗,和化疗) 。最后,我们沿着这根线构画出当前的挑战和我们的观点。 | Yi Liu Jun-Jie Yin Zhihong Nie | 2014 | Nano Research2014，7，12： | 9 |
| 19 | Simultaneous desulfurization and denitrogen of liquid fuels using two functionalized group ionic liquids显示文摘Deep desulfurization of liquid fuels is an important and challenging issue in worldwide petroleum refining industry.Extraction and catalytic oxidative desulfurization(ECODS)of liquid fuels using a series of ionic liquids(ILs)with two functionalized groups,such as[(CH2)2COOHmim]Cl/n Fe Cl3,[(CH2)2COOHmim]Cl/n Zn Cl2,and[Amim]Cl/n Fe Cl3,was studied.In the ECODS,the ILs were used as both extractant and catalyst and 30 wt%hydrogen peroxide(H2O2)solution as oxidant.The effects of molar ratios of[(CH2)2COOHmim]Cl(or[Amim]Cl)to Fe Cl3(or Zn Cl2)in ILs,H2O2/sulfur(O/S)molar ratio,reaction temperature,and the nature of sulfur compounds on sulfur removal were investigated.The natures of the functional groups(–COOH,–CH2–CH=CH2)in cations and the acid strength of anions play important roles in the ECODS and affect the reaction time,temperature,and desulfurization efficiency of different substrates.Also,nitrogen-containing compounds(pyridine,pyrrole,and quinoline)could be removed simultaneously in the ECODS and had different effects on dibenzothiophene removal. | NIE Yi GONG Xue GAO Hong Shuai ZHANG Xiang Ping ZHANG Suo Jiang | 2014 | Science China Chemistry2014，57，12： | 8 |
| 20 | An Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens显示文摘Objective To provide a feasible and cost-effective next-generation sequencing(NGS) method for accurate identification of viral pathogens in clinical specimens, because enormous limitations impede the clinical use of common NGS, such as high cost, complicated procedures, tremendous data analysis, and high background noise in clinical samples. Methods Viruses from cell culture materials or clinical specimens were identified following an improved NGS procedure: reduction of background noise by sample preprocessing, viral enrichment by barcoded oligonucleotide(random hexamer or non-ribosomal hexanucleotide) primer-based amplification, fragmentation-free library construction and sequencing of one-tube mixtures, as well as rapid data analysis using an in-house pipeline. Results NGS data demonstrated that both barcoded primer sets were useful to simultaneously capture multiple viral pathogens in cell culture materials or clinical specimens and verified that hexanucleotide primers captured as many viral sequences as hexamers did. Moreover, direct testing of clinical specimens using this improved hexanucleotide primer-based NGS approach provided further detailed genotypes of enteroviruses causing hand, foot, and mouth disease(HFMD) and identified other potential viruses or differentiated misdiagnosis events. Conclusion The improved barcoded oligonucleotide primer-based NGS approach is simplified, time saving, cost effective, and appropriate for direct identification of viral pathogens in clinical practice. | WANG Churl Hua NIE Kai ZHANG Yi WANG Ji ZHOU Shuai Feng LI Xin Na ZHOU Hang Yu QI Shun Xiang MA Xue Jun | 2017 | Biomedical and Environmental Sciences2017，30，1： | 7 |
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