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| 1 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 2 | Isosteric design of friction-reduction and anti-wear lubricant additives with less sulfur content显示文摘To reduce harmful sulfur content in lubricant additives, making use of isosterism has been shown to be an effective strategy. When thiobenzothiazole compounds were used as templates, the exchange of sulfur atoms in the thiazole ring with oxygen atoms and NH groups produced twelve isosteres. Similarly, 2-benzothiazole- S-carboxylic acid esters were used as template molecules to produce six isosteres. About 30% of the isosteres exhibited a satisfactory deviation of ±5% relative to the template, ignoring the specific changes in the base oils, the differences in molecular structure, and the friction or wear properties. The template molecules and isosteres in triisodecyl trimellitate exhibited better tribological properties than in trimethylolpropane trioleate or bis(2- ethylhexyl) adipate. Comparative molecular field analysis(CoM FA)- and comparative molecular similarity index analysis(CoMSIA)-quantitative structure tribo-ability relationship(QSTR) models were employed to study the correlation of molecular structures between the base oils and additives. The models indicate that the higher the structural similarities of the base oils and additives are, the more synergetic the molecular force fields of the lubricating system are; the molecular force fields creating synergistic effects will improve tribological performance. | Xinlei GAO Denghui LIU Ze SONG Kang DAI | 2018 | Friction2018，6，2： | 7 |
| 3 | Adaptive target and jamming recognition for the pulse doppler radar fuze based on a time-frequency joint feature and an online-updated naive bayesian classifier with minimal risk显示文摘This paper considers the problem of target and jamming recognition for the pulse Doppler radar fuze(PDRF).To solve the problem,the matched filter outputs of the PDRF under the action of target and jamming are analyzed.Then,the frequency entropy and peak-to-peak ratio are extracted from the matched filter output of the PDRF,and the time-frequency joint feature is constructed.Based on the time-frequency joint feature,the naive Bayesian classifier(NBC)with minimal risk is established for target and jamming recognition.To improve the adaptability of the proposed method in complex environments,an online update process that adaptively modifies the classifier in the duration of the work of the PDRF is proposed.The experiments show that the PDRF can maintain high recognition accuracy when the signal-to-noise ratio(SNR)decreases and the jamming-to-signal ratio(JSR)increases.Moreover,the applicable analysis shows that he ONBCMR method has low computational complexity and can fully meet the real-time requirements of PDRF. | Jian Dai Xin-hong Hao Ze Li Ping Li Xiao-peng Yan | 2022 | Defence Technology(防务技术)2022，18，3： | 4 |
| 4 | Generation of tryptophan hydroxylase 2 gene knockout pigs by CRISPR/Cas9-mediated gene targeting显示文摘Unbalanced brain serotonin(5-HT) levels have implications in various behavioral abnormalities and neuropsychiatric disorders. The biosynthesis of neuronal 5-HT is regulated by the rate-limiting enzyme, tryptophan hydroxylase-2(TPH2). In the present study, the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system was used to target the Tph2 gene in Bama mini pig fetal fibroblasts. It was found that CRISPR/Cas9 targeting efficiency could be as high as 61.5%, and the biallelic mutation efficiency reached at38.5%. The biallelic modified colonies were used as donors for somatic cell nuclear transfer(SCNT) and 10 Tph2 targeted piglets were successfully generated. These Tph2 KO piglets were viable and appeared normal at the birth.However, their central 5-HT levels were dramatically reduced, and their survival and growth rates were impaired before weaning. These Tph2 KO pigs are valuable large-animal models for studies of 5-HT deficiency induced behavior abnomality. | Ze Li Hai-Yuan Yang Ying Wang Man-Ling Zhang Xiao-Rui Liu Qiang Xiong Li-Ning Zhang Yong Jin Li-Sha Mou Yan Liu Rong-Feng Li Yi Rao Yi-Fan Dai | 2017 | The Journal of Biomedical Research2017，31，5： | 4 |
| 5 | First experimental constraints on WIMP couplings in the effective field theory framework from CDEX显示文摘We present weakly interacting massive particles(WIMPs) search results performed using two approaches of effective field theory from the China Dark Matter Experiment(CDEX), based on the data from both CDEX-1B and CDEX-10 stages. In the nonrelativistic effective field theory approach, both time-integrated and annual modulation analyses were used to set new limits for the coupling of WIMP-nucleon effective operators at 90% confidence level(C.L.) and improve over the current bounds in the low mχregion. In the chiral effective field theory approach, data from CDEX-10 were used to set an upper limit on WIMP-pion coupling at 90% C.L. We for the first time extended the limit to the m_(χ)<6 GeV/c^(2) region. | Yi Wang Zhi Zeng Qian Yue LiTao Yang KeJun Kang YuanJing Li Mehment Agartioglu HaiPeng An JianPing Chang JingHan Chen YunHua Chen JianPing Cheng Cheng Yi Chiang WenHan Dai Zhi Deng ChangHao Fang XinPing Geng Hui Gong QiuJu Guo XuYuan Guo HongJian He Li He ShengMing He JinWei Hu TuChen Huang HanXiong Huang HaiTao Jia LiPing Jia Xi Jiang HauBin Li JianMin Li Jin Li MingXuan Li RenMingJie Li Xia Li YuLan Li Bin Liao FongKay Lin ShinTed Lin ShuKui Liu YanDong Liu Yu Liu YuanYuan Liu ZhongZhi Liu Hao Ma YuCai Mao QiYuan Nie JinHua Ning Hui Pan NingChun Qi Jie Ren XiChao Ruan ChangSong Shang Vivek Sharma Ze She Lakhwinder Singh Monoj Kumar Singh TianXi Sun ChangJian Tang WeiYou Tang Yang Tian GuangFu Wang Li Wang Qing Wang Yu Chen Wang YunXiang Wang Zhen Wang Henry Tsz-King Wong ShiYong Wu YuCheng Wu HaoYang Xing Yin Xu Tao Xue YuLu Yan Nan Yi ChunXu Yu HaiJun Yu JianFeng Yue Ming Zeng BingTao Zhang Lei Zhang FengShou Zhang ZhenYu Zhang KangKang Zhao MingGang Zhao JiFang Zhou ZuYing Zhou JingJun Zhu | 2021 | Science China(Physics,Mechanics & Astronomy)2021，64，8： | 3 |
| 6 | Total Synthesis of Three New Dihydrostilbenes from Bulbophllum odoratissimum显示文摘A total synthesis of three new dihydrostilbenes, 1, 2 and 3, which were isolated from Bulbophyllum odoratissimum Lindl. with significant cytotoxicity toward human cancer cell lines, was developed via Horner reaction etc. The natural products 1, 2 and 3 were obtained in 5.8%, 6.6% and 5.9%, respectively. | Jin Guang LIN Wei Ge ZHANG Rui ZHAO Ze Yu NIU Kai BAO Dai Lin LIU Nai Li WANG Xin Shen YAO | 2006 | Chinese Chemical Letters2006，17，3： | 1 |
| 7 | Facile synthesis of organo-soluble surface-grafted all-single-layer graphene oxide as hole-injecting buffer material in orga- nic light-emitting diodes 显示文摘 | Zhong Ze Dai Yanfeng Ma Dongge | 2011 | Journal of Materials Che- mistry2011，21，16： | 1 |
| 8 | New synthesis of bis(disiloxanyl)-m-carboranes显示文摘四二度( disiloxanyl ) -m-carboranes (( RSiMe2OSiMe2 ) 2-m-C2B10H10 , R = H ,乙烯基, ethynyl , allyl )从二度( hydroxydimethylsilyl )的反应被综合 -m-carborane 和 disilazanes ,并且由红外线的系列(红外)描绘了,原子磁性的回声( NMR )和集体 spectrometry ( MS )。 | Xue Chao Zhang Ling Hong Kong Hong Jie Sun Yong Xia Tan Li Na Dai Xue Zhong Zhang Zhi Jie Zhang Ze Min Xie | 2012 | Chinese Chemical Letters2012，23，6： | 1 |
| 9 | Facile syn- thesis of organo-soluble surface-grafted all-single-layer graphene oxide as hole-injecting buffer material in organ- ic light-emitting dJodes显示文摘 | ZHONG Ze DAI Yan-feng MA Dong-ge | 2011 | Journal of Materials Chemis- try2011，21，16： | 1 |
| 10 | Facile syn- thesis of organo-soluble surface-grafted all-single-layer graphene oxide as hole-injecting buffer material in or- ganic light-emitting diodes 显示文摘 | Zhong Ze Dai Yanfeng Ma Dongge | 2011 | Journal of Materials Chemistry2011，21，16： | 1 |
| 11 | Facile syn- thesis of ergano-seluble surface-grafted all-single-layer grapheme oxide as hole-injecting buffer material in organ- ic light-emitting diodes显示文摘 | ZHONG Ze DAI Yan-feng MA Dong-ge | 2011 | Journal ef Materials Chemis- try2011，21，16： | 1 |
| 12 | Resistance reduction of patterned surface inspired by cuticle structure of Achalinus spinalis显示文摘The crawling process of snakes is known to have fascinating tribological phenomena,whereas investigations on their frictional properties depending on patterned cuticles are insufficient.In this study,we have designed and fabricated biomimetic microstructures inspired by the geometric microunits of Achalinus spinalis cuticle using polyurethane acrylate(PUA)material and performed its tribological analysis.The micro-morphology of this Achalinus-inspired textured polymer surface(AITPS)is characterized by the closely and evenly quasi-rectangular microgrooves,periodically arranged along certain orientations.We have compared the frictional performance of our fabricated AITPS with other competitive microstructure,using a smooth steel ball and commercial clay as an interacting surface.After performing massive friction tests with steel ball and clay,AITPS still maintains good resistance reduction performed compared to the patterned surface with straight microgrooves,which is most likely due to the reduction of actual contact areas at the frictional interface. | Jiahui ZHAO Keju JI Qin CHEN Muhammad Niaz KHAN Chongwen TU Ze MA Jianming WU Jian CHEN Zhendong DAI | 2023 | Friction2023，11，7： | 0 |
| 13 | An Economic Optimization Method for Demand-side Energy-storage Accident Backup Assisted Deep Peaking of Thermal Power Units显示文摘With the large-scale penetration of new energy such as wind power,its anti-modulation peak characteristics have increased challenges in power systems.Therefore,an economic optimization method for depth peak regulation and the depth of the emergency of the Energy storage(ES)accident on the demand side is proposed.First,a quantitative model of unplanned disconnection risk caused by weather state,load level,and fault type is constructed to obtain the spare and available ES capacity.Therefore,a deep peak regulation(DPR)economic optimization model is established to minimize the fuel injection cost of thermal power units,including ES accident standby,unit damage,and fuel demand.The particle swarm optimization algorithm is used to simulate the modified IEEE 30-node system.Based on the results,the DPR of ES accident standby can reduce the wind abandonment rate by 1.1%and the total peak adjustment cost by 33.5%under class-A weather.In class-C weather,the wind abandonment ratio can be reduced by 4.19%,reducing the cost of the total adjustment peak by 31.4%.The multiple purposes of improving the power grid modulation,wind power,and the standby utilization of ES accidents can be achieved. | Wen Wei Ze Ye Yali Wang Shuangfeng Dai Lei Chen Xiaolong Liu | 2022 | Chinese Journal of Electrical Engineering2022，8，2： | 0 |
| 14 | Advances in nuclear detection and readout techniques显示文摘“A Craftsman Must Sharpen His Tools to Do His Job,”said Confucius.Nuclear detection and readout techniques are the foundation of particle physics,nuclear physics,and particle astrophysics to reveal the nature of the universe.Also,they are being increasingly used in other disciplines like nuclear power generation,life sciences,environmental sciences,medical sciences,etc.The article reviews the short history,recent development,and trend of nuclear detection and readout techniques,covering Semiconductor Detector,Gaseous Detector,Scintillation Detector,Cherenkov Detector,Transition Radiation Detector,and Readout Techniques.By explaining the principle and using examples,we hope to help the interested reader underst and this research field and bring exciting information to the community. | Rui He Xiao‑Yang Niu Yi Wang Hong‑Wei Liang Hong‑Bang Liu Ye Tian Hong‑Lin Zhang Chao‑Jie Zou Zhi‑Yi Liu Yun‑Long Zhang Hai‑Bo Yang Ju Huang Hong‑Kai Wang Wei‑Jia Han Bei Cao Gang Chen Cong Dai Li‑Min Duan Rui‑Rui Fan Fang‑Fa Fu Jian‑Hua Guo Dong Han Wei Jiang Xian‑Qin Li Xin Li Zhuo‑Dai Li Yu‑Tie Liang Shun Liao De‑Xu Lin Cheng‑Ming Liu Guo‑Rui Liu Jun‑Tao Liu Ze Long Meng‑Chen Niu Hao Qiu Hu Ran Xiang‑Ming Sun Bo‑Tan Wang Jia Wang Jin‑Xiang Wang Qi‑Lin Wang Yong‑Sheng Wang Xiao‑Chuan Xia Hao‑Qing Xie He‑Run Yang Hong Yin Hong Yuan Chun‑Hui Zhang Rui‑Guang Zhao Ran Zheng Cheng‑Xin Zhao | 2023 | Nuclear Science and Techniques2023，34，12： | 0 |
| 15 | Enhancing boll protein synthesis and carbohydrate conversion by the application of exogenous amino acids at the peak flowering stage increased the boll Bt toxin concentration and lint yield in cotton显示文摘In Bacillus thuringenesis(Bt) transgenic cotton, the cotton boll has the lowest insecticidal protein content when compared to the other organs. The present study investigated the effects of amino acid spray application at the peak flowering stage on the cotton boll Bt toxin concentration and yield formation. Boll protein synthesis and carbohydrate conversion were also studied to reveal the fundamental mechanism. Three treatments(i.e., CK, the untreated control;LA1, five amino acids;LA2, 21 amino acids) were applied to two Bt cultivars of G. hirsutum(i.e., the hybrid Sikang 3 and the conventional Sikang 1) in the cotton-growing seasons during 2017 and 2018. Amino acid spray application at the peak flowering stage resulted in an increase of 5.2–16.4% in the boll Bt protein concentration and an increase of 5.5–11.3%in the seed cotton yield, but there was no difference between the two amino acid treatments. In addition, amino acid applications led to increases in the amino acid content, soluble protein content, glutamate pyruvate transaminase(GPT)activity, glutamate oxaloacetate transaminase(GOT) activity, glucose content, fructose content and soluble acid invertase(SAI) activity. This study also found that Bt protein content, enhanced boll number and the weight of opened bolls were closely related to carbon and nitrogen metabolism. The Bt protein content had significant linear positive correlations with amino acid and soluble protein contents. Enhanced boll number had significant linear positive correlations with the GPT and GOT activities from 15–25 days after flowering(DAF). The weight of opened bolls from 55–65 DAF had a significant linear positive correlation with the SAI activity. These results indicate that the enhancement of boll protein synthesis and carbohydrate conversion by amino acid application resulted in a simultaneous increase in the boll Bt protein concentration and cotton lint yield. | LIU Zhen-yu LI Yi-yang Leila.I.M.TAMBEL LIU Yu-ting DAI Yu-yang XU Ze LENG Xin-hua ZHANG Xiang CHEN De-hua CHEN Yuan | 2023 | Journal of Integrative Agriculture2023，22，6： | 0 |
| 16 | Exploration of KrF Excimer Laser Pulse Compress显示文摘The technique of KrF excimer laser pulse compression was explored.The amplified self emission (ASE) pulse was compressed successfully with two discharge pumped excimer laser.The laser pulse from | GAO Zhi-xing,TANG xiu-zhang,ZHANG Hai-feng,LU Ze,HU Feng-ming,LI Jing,XIANG Yi-huai,DAI hui | 2009 | Annual Report of China Institute of Atomic Energy2009，，1： | 0 |
| 17 | Bone-targeting engineered small extracellular vesicles carrying anti-miR-6359-CGGGAGC prevent valproic acid-induced bone loss显示文摘The clinical role and underlying mechanisms of valproic acid(VPA)on bone homeostasis remain controversial.Herein,we confirmed that VPA treatment was associated with decreased bone mass and bone mineral density(BMD)in both patients and mice.This effect was attributed to VPA-induced elevation in osteoclast formation and activity.Through RNA-sequencing,we observed a significant rise in precursor miR-6359 expression in VPA-treated osteoclast precursors in vitro,and further,a marked upregulation of mature miR-6359(miR-6359)in vivo was demonstrated using quantitative real-time PCR(qRT-PCR)and miR-6359 fluorescent in situ hybridization(miR-6359-FISH).Specifically,the miR-6359 was predominantly increased in osteoclast precursors and macrophages but not in neutrophils,T lymphocytes,monocytes and bone marrow-derived mesenchymal stem cells(BMSCs)following VPA stimulation,which influenced osteoclast differentiation and bone-resorptive activity.Additionally,VPA-induced miR-6359 enrichment in osteoclast precursors enhanced reactive oxygen species(ROS)production by silencing the SIRT3 protein expression,followed by activation of the MAPK signaling pathway,which enhanced osteoclast formation and activity,thereby accelerating bone loss.Currently,there are no medications that can effectively treat VPA-induced bone loss.Therefore,we constructed engineered small extracellular vesicles(E-sEVs)targeting osteoclast precursors in bone and naturally carrying anti-miR-6359 by introducing of EXOmotif(CGGGAGC)in the 3’-end of the anti-miR-6359 sequence.We confirmed that the E-sEVs exhibited decent bone/osteoclast precursor targeting and exerted protective therapeutic effects on VPA-induced bone loss,but not on ovariectomy(OVX)and glucocorticoid-induced osteoporotic models,deepening our understanding of the underlying mechanism and treatment strategies for VPA-induced bone loss. | Xudong Xie Peng Cheng Liangcong Hu Wu Zhou Detai Zhang Samuel Knoedler Guodong Liu Yuan Xiong Hang Xue Yiqiang Hu Barbara Kern Doha Obed Adriana CPanayi Lang Chen Chenchen Yan Ze Lin Guandong Dai Bobin Mi Yingze Zhang Guohui Liu | 2024 | Signal Transduction and Targeted Therapy2024，9，2： | 0 |
| 18 | Desalted duck egg white nanogels as Pickering stabilizers for food-grade oil-in-water emulsion显示文摘Achieving the reuse of traditional egg by-products,salted duck egg whites(SEW),is an urgent problem to be solved.In this current work,we constructed a heat-induced gel-assisted desalination method for SEW.Subsequently,a top-down way was utilized to prepare desalted duck egg protein nanogels(DEPN)with uniformly distributed diameters and their application in the oil/water(O/W)interface system was explored.The results revealed that the increase of DEPN concentration could lower the droplet size,however,the size was negatively correlated with the oil phase fraction.Moreover,the effect of pH,ionic strength,and temperature on the emulsion stability demonstrated that the DEPN-stabilized emulsion displayed superior physical stability under different conditions.The addition of NaCl resulted in the significant decrease in droplet size of the emulsion,while further increasing the NaCl concentration,the droplet size did not decrease accordingly.Besides,heat-treatment and cold-treatment had little negative effect on the stability of the emulsion.Even if the droplet size of the emulsion increased at 80℃for 3 h,the morphology of the emulsion remained unchanged.Our study demonstrated DEPN had great potential as a stabilizer for food-grade Pickering emulsions. | Jingyun Zhao Xiaohan Guo Ze Chen Yalei Dai Hongshan Liang Qianchun Deng Shugang Li Bin Zhou | 2022 | Food Science and Human Wellness2022，11，5： | 0 |
| 19 | THE NMR CONFORMATIONAL STUDIES OF BACITRACIN A显示文摘Bacitracin consists of a mixture of several closely related polypeptide antibiotics produced from Bacillus licheniformis.The major component, Bacitracin A, is a cyclic dodecapeptide(Fig, 1), which has strong bacteriocidal effect on the G+ bacteria. The bacteriocidal activity requires the presence of divalent cations such as Zn(?)+, As we know, the biological function of ploypeptides and proteins are closely related to their three-dimensional structure. Bacitracin A is very important for the demonstration of the coordination site of metal and the relationship | Xiu Weng HAN~*, Jian Yi NI, Xi Jia MIAO and Jie Han HU Dalian Institute of Chemical Physics, Academis Sinica, 116023. Laboratory of Applied Organic Chemistry, Lanzhou University, 730000 Jian ZHUANG, Guan Hong WENG and Xiao Mao ZHONG National Key Laboratory of Bio-Organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Academia Sinica, 200032 Jun Den YANG and Xiang Peng DAI,#:Hong WANG and Chuan Ze SHEN Shenyang Institute of Ecology, #: Shenyang Institute of Automation, Academia Siniea, 110015 | 1993 | Chinese Chemical Letters1993，4，5： | 0 |
| 20 | Eight Zhes Decoction ameliorates the lipid dysfunction of nonalcoholic fatty liver disease using integrated lipidomics, network pharmacology and pharmacokinetics显示文摘Nonalcoholic fatty liver disease(NAFLD)has developed into the most common chronic liver disease and can lead to liver cancer.Our laboratory previously developed a novel prescription for NAFLD,“Eight Zhes Decoction”(EZD),which has shown good curative effects in clinical practice.However,the pharmacodynamic material basis and mechanism have not yet been revealed.A strategy integrating lipidomics,network pharmacology and pharmacokinetics was used to reveal the active components and mechanisms of EZD against NAFLD.The histopathological results showed that EZD attenuated the degrees of collagen deposition and steatosis in the livers of nonalcoholic steatofibrosis model mice.Furthermore,glycerophospholipid metabolism,arachidonic acid metabolism,glycerolipid metabolism and linoleic acid metabolism with phospholipase A2 group IVA(PLA2G4A)and cytochrome P450 as the core targets and 12,13-cis-epoxyoctadecenoic acid,12(S)-hydroxyeicosatetraenoic acid,leukotriene B4,prostaglandin E2,phosphatidylcholines(PCs)and triacylglycerols(TGs)as the main lipids were found to be involved in the treatment of NAFLD by EZD.Importantly,naringenin,artemetin,canadine,and bicuculline were identified as the active ingredients of EZD against NAFLD;in particular,naringenin reduces PC consumption by inhibiting the expression of PLA2G4A and thus promotes sufficient synthesis of very-low-density lipoprotein to transport excess TGs in the liver.This research provides valuable data and theoretical support for the application of EZD against NAFLD. | Yuping Zhou Ze Dai Kaili Deng Yubin Wang Jiamin Ying Donghui Chu Jinyue Zhou Chunlan Tang | 2023 | Journal of Pharmaceutical Analysis2023，13，9： | 0 |
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