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| 1 | Copy number increase of aurora kinase A in colorectal cancers: a correlation with tumor progression显示文摘联系中心体的 kinase 曙光 A (AURKA ) 涉及基因不稳定性并且在包括 colorectal 癌症(CRC ) 的几人的癌是过去表示的。AURKA 的 choromosome 地点, 20q13,经常在 CRC,和广泛地在大量临床的样品要调查的如此的区域需要的功能的影响被放大。colorectal 腺癌和邻近的正常上皮的匹配盒子的纸巾(n= 134 ) 在这研究被包括。量的 PCR 被执行在 CRC 检验拷贝数字和 AURKA 的 mRNA 水平。我们的结果证明 AUKRA 的拷贝数字获得在 CRC 样品的一个相对高百分比被检测(32.4% , 134 中的 43 个) 。在 AURKA 和肿瘤前进的拷贝数字增加之间有积极关联。并且 AURKA 的拷贝数字获得也在 CRC 与 mRNA 在表示上显示出积极关联。然而, AURKA mRNA 的表示水平也与未改变的拷贝数字在 CRC 样品的组被提高。这些调查结果显示分散的 colorectal 癌症展出一条规定和这可以影响的曙光的不同机制指向曙光的治疗的功效。 | Chao Zhang Zhengyu Fang Yi Xioong Jiana Li Li Liu Manhui Li Wei Zhang Jun Wan | 2010 | Acta Biochimica et Biophysica Sinica2010，42，11： | 4 |
| 2 | Effects of Epidural Spinal Cord Stimulation and Treadmill Training on Locomotion Function and Ultrastructure of Spinal Cord Anterior Horn after Moderate Spinal Cord Injury in Rats显示文摘Objective:To investigate the effects of epidural spinal cord stimulation(ESCS) and treadmill training on the locomotion function and ultrastructure of spinal cord anterior horn after moderate spinal cord injury in rats.Method:Nine adult female Sprague-Dawley rats were randomly distributed into three groups:①spinal cord injury group(SI,n=3).②spinal cord injury plus ESCS group(SE,n=3).③spinal cord injury plus treadmill training group(TT,n=3).All rats received a moderate spinal cord injury surgery.Four weeks after surgery,rats in SE group received an electrode implantation procedure,with the electrode field covering spinal cord segments L2-S1.Four weeks after electrode implantation,rats received subthreshold ESCS for 30 min/d.Rats in TT group received 4cm/s treadmill training for 30min/d.Rats in SI group received no intervention,as a control group.All procedures in these three groups lasted four weeks.The open field Basso,Beattie and Bresnahan(BBB) scale was used before and after intervention to evaluate rats' hindlimb motor function.Result:After four weeks intervention,rats in TT group improved their open field locomotion scores to 20.In contrast,no significant improvement was observed in groups SI and SE.The morphology of synapses and neurons were similar regardless of whether rats had undergone ESCS,treadmill training or not.Conclusion:ESCS alone was not sufficient to improve the walking ability of spinal cord injured rats.ESCS or treadmill training alone might not contribute to the changes of ultrastructure in anterior horn of spinal cord that underlie the recovery of walking ability.Further research is needed to understand the contributions of combination of ESCS and treadmill training to the rehabilitation of spinal cord injured rats. | WANG Yizhao HUANG Xiaolin XU Jiang XU Tao FANG Zhengyu XU Qi TU Xikai YANG Peipei | 2009 | 中国康复医学杂志2009，24，6： | 4 |
| 3 | D-Galactose-caused life shortening in Drosophila melanogaster and Musca domestica is associated with oxidative stress显示文摘 | Xu Cui Luning Wang Pingping Zuo Zhitao Han Zhengyu Fang Wenbin Li Jiankang Liu | 2004 | Biogerontology2004，，5： | 1 |
| 4 | D-Galactose-caused life shortening in Drosophila melanogaster and Musca domestica is associated with oxidative stress显示文摘 | Xu Cui Luning Wang Pingping Zuo Zhitao Han Zhengyu Fang Wenbin Li Jiankang Liu | 2004 | Biogerontology2004，，5： | 1 |
| 5 | Photodegradation of riboflavin initiated DNA damage and gene expression in Hela cells显示文摘 | Dong J Tian Yaping Fang Zhengyu | 2004 | J Tumor Maker Oncology2004，19，1： | 1 |
| 6 | Amplification and up-regulation of microRNA-30b in oral squamous cell cancers显示文摘 | Chenying Shao Yili Yu Liying Yu Yuan Pei Qiping Feng Fengting Chu Zhengyu Fang Yi Zhou | 2012 | Archives of Oral Biology2012，，8： | 1 |
| 7 | Beneficial effects of a decreased meal frequency on nutrient utilization,secretion of luteinizing hormones and ovarian follicular development in gilts显示文摘Background:Replacement gilts are typically fed ad libitum,whereas emerging evidence from human and rodent studies has revealed that time-restricted access to food has health benefits.The objective of this study was to investigate the effect of meal frequency on the metabolic status and ovarian follicular development in gilts.Methods:A total of 36 gilts(Landrace×Yorkshire)with an age of 150±3 d and a body weight of 77.6±3.8 kg were randomly allocated into one of three groups(n=12 in each group),and based on the group allocation,the gilts were fed at a frequency of one meal(T1),two meals(T2),or six meals per day(T6)for 14 consecutive weeks.The effects of the meal frequency on growth preference,nutrient utilization,short-chain fatty acid production by gut microbial,the post-meal dynamics in the metabolic status,reproductive hormone secretions,and ovarian follicular development in the gilts were measured.Results:The gilts in the T1 group presented a higher average daily gain(+48 g/d,P<0.05)and a higher body weight(+4.9 kg,P<0.05)than those in the T6 group.The meal frequency had no effect on the apparent digestibility of dry matter,crude protein,ether extract,ash,and gross energy,with the exception that the T1 gilts exhibited a greater NDF digestibility than the T6 gilts(P<0.05).The nitrogen balance analysis revealed that the T1 gilts presented decreased urine excretion of nitrogen(−8.17 g/d,P<0.05)and higher nitrogen retention(+9.81 g/d,P<0.05),and thus exhibited higher nitrogen utilization than the T6 gilts.The time-course dynamics of glucose,α-amino nitrogen,urea,lactate,and insulin levels in serum revealed that the T1 group exhibited higher utilization of nutrients after a meal than the T2 or T6 gilts.The T1 gilts also had a higher acetate content and SCFAs in feces than the T6 gilts(P<0.05).The age,body weight and backfat thickness of the gilts at first estrous expression were not affected by the meal frequency,but the gilts in the T1 group had higher levels of serum luteinizing hormone on the 18th day of the 3rd estrus cycle and 17β-estradiol,a larger number of growing follicles and corpora lutea,and higher mRNA expression levels of genes related to follicular development on the 19th day of the 3rd estrus cycle.Conclusions:The current findings revealed the benefits of a lower meal frequency equal feed intake on nutrient utilization and reproductive function in replacement gilts,and thus provide new insights into the nutritional strategy for replacement gilts,and the dietary pattern for other mammals,such as humans. | Lun Hua Lianpeng Zhao Zhengyu Mao Wentao Li Jing Li Xuemei Jiang Lianqiang Che Shengyu Xu Yan Lin Zhengfeng Fang Bin Feng De Wu Yong Zhuo | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 1 |
| 8 | Brake wear-derived particles:Single-particle mass spectral signatures and real-world emissions显示文摘Brake wear is an important but unregulated vehicle-related source of atmospheric particulate matter(PM).The single-particle spectral fingerprints of brake wear particles(BWPs)provide essential information for understanding their formation mechanism and atmospheric contributions.Herein,we obtained the single-particle mass spectra of BWPs by combining a brake dynamometer with an online single particle aerosol mass spectrometer and quantified real-world BWP emissions through a tunnel observation in Tianjin,China.The pure BWPs mainly include three distinct types of particles,namely,Bacontaining particles,mineral particles,and carbon-containing particles,accounting for 44.2%,43.4%,and 10.3%of the total BWP number concentration,respectively.The diversified mass spectra indicate complex BWP formation pathways,such as mechanical,phase transition,and chemical processes.Notably,the mass spectra of Ba-containing particles are unique,which allows them to serve as an excellent indicator for estimating ambient BWP concentrations.By evaluating this indicator,we find that approximately 4.0%of the PM in the tunnel could be attributable to brake wear;the real-world fleet-average emission factor of 0.28 mg km1 veh1 is consistent with the estimation obtained using the receptor model.The results presented herein can be used to inform assessments of the environmental and health impacts of BWPs to formulate effective emissions control policies. | Jiayuan Liu Jianfei Peng Zhengyu Men Tiange Fang Jinsheng Zhang Zhuofei Du Qijun Zhang Ting Wang Lin Wu Hongjun Mao | 2023 | Environmental Science and Ecotechnology2023，，3： | 0 |
| 9 | Immunity-and-matrix-regulatory cells enhance cartilage regeneration for meniscus injuries: a phase I dose-escalation trial显示文摘Immunity-and-matrix-regulatory cells(IMRCs)derived from human embryonic stem cells have unique abilities in modulating immunity and regulating the extracellular matrix,which could be mass-produced with stable biological properties.Despite resemblance to mesenchymal stem cells(MSCs)in terms of self-renew and tri-lineage differentiation,the ability of IMRCs to repair the meniscus and the underlying mechanism remains undetermined.Here,we showed that IMRCs demonstrated stronger immunomodulatory and pro-regenerative potential than umbilical cord MSCs when stimulated by synovial fluid from patients with meniscus injury.Following injection into the knees of rabbits with meniscal injury,IMRCs enhanced endogenous fibrocartilage regeneration.In the dose-escalating phase I clinical trial(NCT03839238)with eighteen patients recruited,we found that intra-articular IMRCs injection in patients was safe over 12 months post-grafting.Furthermore,the effective results of magnetic resonance imaging(MRI)of meniscus repair and knee functional scores suggested that 5×107 cells are optimal for meniscus injury treatment.In summary,we present the first report of a phase I clinical trial using IMRCs to treat meniscus injury.Our results demonstrated that intra-articular injection of IMRCs is a safe and effective therapy by providing a permissive niche for cartilage regeneration. | Liangjiang Huang Song Zhang Jun Wu Baojie Guo Tingting Gao Sayed Zulfiqar Ali Shah Bo Huang Yajie Li Bo Zhu Jiaqi Fan Liu Wang Yani Xiao Wenjing Liu Yao Tian Zhengyu Fang Yingying Lv Lingfeng Xie Sheng Yao Gaotan Ke Xiaolin Huang Ying Huang Yujuan Li Yi Jia Zhongwen Li Guihai Feng Yan Huo Wei Li Qi Zhou Jie Hao Baoyang Hu Hong Chen | 2023 | Signal Transduction and Targeted Therapy2023，8，12： | 0 |
| 10 | novel amylolytic enzyme from Palaeococcus ferrophilus with malto-oligosaccharide forming ability belonging to subfamily GH13_20显示文摘By heterologous expression of a gene from Palaeococcus ferrophilus,a novel recombinant enzyme designated AMPf was obtained and proved to be an amylolytic enzyme with unique catalytic characteristics.The optimal temperature and pH of AMPf were 50℃ and 7.0 respectively.Although sequence analysis and acarbose hydrolysis ability indicated that AMPf belongs to the subfamily GH13_20,interestingly,this enzyme hardly acts on cyclodextrins and pullulan distinguishing it from most enzymes in this subfamily.AMPf hydrolyzes starches to glucose,maltose,maltotriose,and maltotetrose as main products.AMPf mainly liberates glucose from starch with the concentration of 1%(w/v),while it shows malto-oligosaccharide forming ability with higher starch concentration of 4%(w/v).Also,the 4,6-ethylidene-4-nitrophenyl-maltoheptaose hydrolysis ability further indicates the unique combination endo-acting and glucose releasing exo-acting activtity of AMPf.AMPf could utilize maltose and maltotriose to produce malto-oligosaccharides by transglycosylation activity.It was proven AMPf has application protential in malto-oligosaccharides production. | Hangyan Ji Xiaoxiao Li Tong Jiang Qi Fang Yuxiang Bai Jie Long Long Chen Zhengyu Jin | 2022 | Food Bioscience2022，45，1： | 0 |
| 11 | Differentiation of the Agonists and Antagonists of the α7 Nicotinic Acetylcholine Receptor显示文摘Theα7 nicotinic acetylcholine receptors(nAChRs)are widely expressed in the central and peripheral nervous systems and are important drug targets for the treatment of neurological diseases.However,differentiation of the agonists and antagonists of the nAChR is difficult.In this study we aimed to develop a reliable and efficient computational approach for differentiation of the agonists from the antagonists of the nAChR based on a systematical analysis of 123 ligands(87 agonists,12 partial agonists,and 24 antagonists)binding with the extracellular domain of theα7 n AChR chimera.Our results suggest that the ligand size and ligand binding affinity cannot differentiate the agonists from the antagonists of the nAChR.The ligand efficiency that considers both ligand binding affinity and size for the agonists is overall more left shifted in comparison to the antagonists,but the values of the ligand efficiency still cannot differentiate the agonists from the antagonists unless the values are either relatively high(more than-0.3 kcal mol^-1)or relatively low(less than-0.45 kcal mol^-1).Our results suggest that accurate prediction of the agonist or antagonist of the nAChR is challenging and the ligand innate configuration has to be considered as an extra for differentiation of the agonists from the antagonists of the nAChR. | WU Guanzhao XU Qingliang BAO Yilei LIU Yuwei LI Qian FANG Zhengyu FU Jingyi DING Yuhang LIANG Zhiqing JIANG Tao YU Rilei | 2019 | Journal of Ocean University of China2019，18，5： | 0 |
| 12 | Characteristics of high frequency radio wave propagated in heated ionospheric regions显示文摘A two-dimensional Ohm heating theoretic model in the magnetizing ionosphere and a ray-tracing model in a discrete ionosphere background are used to analyze quantitatively the characteristics(mainly the Doppler shift and the phase shift)of the over-the-horizon radar(OTHR)wave,which propagates through the ionospheric region heated by high frequency radio wave.The simulation results about the Doppler and the phase shift are obtained within two minutes after the heater is on.Preliminary conclusions are given by comparing the numerical results with experimental data. | Fang HE Zhengyu ZHAO | 2009 | Frontiers of Electrical and Electronic Engineering in China2009，4，2： | 0 |
| 13 | Preprocessing of ionospheric echo Doppler spectra显示文摘The real-time information of the distant ionosphere can be acquired by using the Wuhan ionospheric oblique backscattering sounding system(WIOBSS),which adopts a discontinuous wave mechanism.After the characteristics of the ionospheric echo Doppler spectra were analyzed,the signal preprocessing was developed in this paper,which aimed at improving the Doppler spectra.The results indicate that the preprocessing not only makes the system acquire a higher ability of target detection but also suppresses the radio frequency interference by 6-7 dB. | FANG Liang ZHAO Zhengyu WANG Feng SU Fanfan | 2007 | Frontiers of Electrical and Electronic Engineering in China2007，2，4： | 0 |
| 14 | Study on the ionospheric effects with different heat-conditions显示文摘A numerical model has been developed.Based on the numerical simulation results,the spatial effects of the ionosphere,mainly consisting of the change on electron density(ED)and electron temperature(ET),heated by the high frequency(HF)pump wave have been analyzed quantitatively.Results are presented as the space-time evolution regulation on the main parameters of the ionosphere resulted by the HF heating waves under the different heat-conditions,just as different regions,such as high latitude and mid-low latitude;different heating power or frequency,such as underdense heating and over-dense heating and regions at different altitudes.The heating effects in different regions with different heating conditions have been presented in figures.Finally,some primary conclusions are given by comparing the simulation results with experimental observation. | HE Fang ZHAO Zhengyu NI Binbin ZHANG Yuannong | 2007 | Frontiers of Electrical and Electronic Engineering in China2007，2，4： | 0 |
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