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| 1 | Efficacy of Zhonglun'a-decoction-containing serum on fibroblastlike synoviocyte apoptosis in rats with collagen-induced arthritis via inhibiting Janus kinase/signal transducer and activator of transcription signaling pathway显示文摘OBJECTIVE: To investigate the efficacy of Zhonglun'a-decoction-containing serum(ZHONGL-CS)on the in vitro apoptosis of fibroblast-like synoviocytes(FLS) from rats with collagen-induced arthritis(CIA) by investigating the Janus kinase/signal transducer and activator of transcription(JAK/STAT) signaling pathway.METHODS: A CIA rat model was established using bovine type Ⅱ collagen. FLS were isolated, cultured and identified. A cell counting kit-8 was used to detect cell activity. The half maximal inhibitory concentration(IC50) was calculated. Experimental subjects were divided into control, CIA,ZHONGL-CS, JAK2 inhibitor AG490, and ZHONGLCS with AG490 groups. The in vitro cell cycle and apoptosis rate were detected in FLS by flow cytometry. Bcl-2-associated X protein(Bax), B-cell lymphoma 2(Bcl-2), caspase-3, cyclin D1, phosphorylated JAK2, STAT1, and STAT3 protein expressions in FLS were examined by Western blotting. JAK2, STAT1 and STAT3 mRNA levels were examined by quantitative real-time polymerase chain reaction.RESULTS: Compared with the CIA group, FLS proliferation was inhibited, the FLS G0/G1 cell cycle was arrested, and the rate of FLS apoptosis was increased in the ZHONGL-CS group. In the ZHONGLCS group, the protein levels of Bcl-2 and cyclin D1 were reduced compared with the CIA group and the levels of Bax and caspase-3 in FLS were increased. In the ZHONGL-CS group, the expressions of JAK2, STAT1, and STAT3 mRNA and the levels of phosphorylated JAK2, STAT1, and STAT3 proteins were reduced.CONCLUSION: ZHONGL-CS may induce FLS apoptosis in CIA rats. Activation of the JAK/STAT signaling pathway was inhibited in FLS in vitro. | Yang Yongsheng Dong Qiumei Ma Chunjie Zhang Yudi Li Yongle Chen Kun Zhang Suo | 2019 | Journal of Traditional Chinese Medicine2019，39，2： | 4 |
| 2 | Rheological responses of fumed silica suspensions under steady and oscillatory shear显示文摘Rheological experiments under steady and oscillatory shear were conducted for fumed silica suspen- sions in polyethylene glycol. Under steady shear the shear-thinning and thickening response were exhibited and the flow exponent N was determined. With the increase of concentration the flow exponent N showed a rapid increase, and it increased dramatically when the discontinuous shear-thickening took place. Oscillatory shear experiments were conducted at constant frequency and constant amplitude strain, respectively. The shear-thinning and the discontinuous shear-thickening behavior were observed under different constant frequencies from 10 to 80 rad/s. The correlation between complex modulus (G*) and sweep frequency (ω) was illuminated at γ =750%. It was found that the correlation between G* and ω could be fitted by equation: G*∝ωn. The indexes in shear-thinning region and shear-thickening were determined. The indexes were similar to some extent at shear-thinning region and increased dramati- cally to a much higher value when the shear-thickening occurred,especially at higher weight fractions. The behaviors can be qualitatively explained as follows: the shear-thinning owes to decrease of viscos- ity, which results from disruption of the aggregates; the cluster theory attributes the shear-thickening to the formation of metastable, flow induced clusters, which block the system. | YANG HaiLin RUAN JianMing ZOU JianPeng WU QiuMei ZHOU ZhongCheng ZHOU ZhiHua | 2009 | Science China(Technological Sciences)2009，52，4： | 2 |
| 3 | A NOTE ON RICHARDSON EXTRAPOLATION OF GALERKIN METHODS FOR EIGENVALUE PROBLEMS OF FREDHOLM INTEGRAL EQUATIONS显示文摘In this paper,we introduce a new extrapolation formula by combining Richardson extrapolationand Sloan iteration algorithms.Using this extrapolation formula,we obtainsome asymptotic expansions of the Galerkin finite element method for semi-simple eigenvalueproblems of Fredholm integral equations of the second kind and improve the accuracyof the numerical approximations of the corresponding eigenvalues.Some numerical experimentsare carried out to demonstrate the effectiveness of our new method and to confirmour theoretical results. | Qiumei Huang Yidu Yang | 2008 | Journal of Computational Mathematics2008，26，4： | 2 |
| 4 | Isolation and Identification of Photobacterium damselae subsp.damselae(PDD) from Tongue Sole显示文摘In May 2016,an epizootic occured among cultured tongue soles caused mass deaths in a fish farm in Qinhuangdao,China.In order to find out the etiological agent,a bacterial strain was isolated from ascites and other tissues of sick tongue sole aseptically collected.The isolate was identified as Photobacterium damselae subsp.damsela(PDD) by isolation culture,Gram staining,physiological identification,morohological observation,biochemical identification and 16S rDNA sequence analysis.The results showed that the isolate shared 99.6% homology with the reference strain in GenBank.The animal regression test displayed that the isolate had very strong pathogenicity to tongue sole.The LD_(50) was 3.1 × 10~4 CFU/mL,and it showed pathogenicity to mammals.The antimicrobial susceptibility test showed the isolate was highly sensitive to nrofloxacin,Norfloxacin,Ciprofloxacin,Mequindox;moderately sensitive to Cefradine,Doxycycline;and insensitive to Gentamicin,Ceftriaxone,Tilmicosin,etc.. | Yang Nan Zhang Zhiqiang Wu Tonglei Zhu Jianxin Fu Yanfang Han Hongsheng Wang Hongbin Shi Qiumei Gao Guisheng | 2018 | Animal Husbandry and Feed Science2018，10，2： | 2 |
| 5 | A posteriori error estimator for spectral approximations of completely continuous operators显示文摘 | Yidu Yang Qiumei Huang | 2006 | Journal of Numerical Analysis and Modeling2006，3，3： | 1 |
| 6 | Particle Sizes and Antibacterial Activity of Radix Astragalus and Radix Isatidis Ultrafine Powders显示文摘Using 9200 laser particle size analyzer and KYKY-2800 scanning electron microscope,particle sizes and cellular morphology of Radix Astragalus and Radix Isatidis ultrafine powders were observed. According to the results,the particle size of 89.1% of Radix Astragalus ultrafine powders ranged from 1.729 μm to 44. 938 μm,D_(50)=4.368 μm; t he particle size of 93.411% of Radix Isatidis ultrafine powders ranged from 1. 510 μm to 44. 938 μm,D50= 8.726 μm. Radix Astragalus and Radix Isatidis ultrafine powders were pulverized completely without intact cellular morphology. The antibacterial activity of Radix Astragalus and Radix Isatidis ultrafine powders against chicken-derived E. coli(O78) w as investigated. The results indicated that Radix Astragalus and Radix Isatidis ultrafine powders exhibited higher antibacterial activity against chicken-derived E. coli(O78) c ompared with the corresponding coarse powders. This study laid a solid foundation for the development and application of Chinese medicine ultrafine powder preparations. | Qiumei SHI Xinhua SHAO Xiumin WANG Xia MENG Xiaoqiao HOU Baoxin YANG Leiyu GUO Jinglong GAO | 2016 | Agricultural Biotechnology2016，5，4： | 1 |
| 7 | 4-14 Production of Projectile and Target K-vacancy in Near-symmetric Collisions by 60100 MeV Cu9+ Ions with Thin Zn Target显示文摘The study on ion-solid collisions has important significances not only for fundamental researches but also for technical applications, like ion-beam modification and analysis of surfaces[1]. So far, K-shell ionization has beenmost widely studied for large range of projectile number and incident energy[2;3]. However, even today, a complete theory that can predict K-shell vacancy production for all collision systems in different velocities is still lacking, especially at an intermediate energy. | Guo Yipan Yang Zhihu Du Shubin Chang Hongwei Xia Qingliang Xu Qiumei | 2015 | IMP & HIRFL Annual Report2015，，1： | 0 |
| 8 | Extreme Ultrviolet Spectra of Highy Stripped Iron Ions显示文摘 | Yang Zhihu Du Shubin Chang Hongwei Zhang Yanping Zhang Boli Xu Qiumei | 2009 | 近代物理研究所和兰州重离子加速器实验室年报:英文版2009，，1： | 0 |
| 9 | Feasibility and physics potential of detecting ^(8)B solar neutrinos at JUNO显示文摘The Jiangmen Underground Neutrino Observatory(JUNO)features a 20 kt multi-purpose underground liquid scintillator sphere as its main detector.Some of JUNO's features make it an excellent location for^8B solar neutrino measurements,such as its low-energy threshold,high energy resolution compared with water Cherenkov detectors,and much larger target mass compared with previous liquid scintillator detectors.In this paper,we present a comprehensive assessment of JUNO's potential for detecting^8B solar neutrinos via the neutrino-electron elastic scattering process.A reduced 2 MeV threshold for the recoil electron energy is found to be achievable,assuming that the intrinsic radioactive background^(238)U and^(232)Th in the liquid scintillator can be controlled to 10^(-17)g/g.With ten years of data acquisition,approximately 60,000 signal and 30,000 background events are expected.This large sample will enable an examination of the distortion of the recoil electron spectrum that is dominated by the neutrino flavor transformation in the dense solar matter,which will shed new light on the inconsistency between the measured electron spectra and the predictions of the standard three-flavor neutrino oscillation framework.IfDelta m^(2)_(21)=4.8times10^(-5);(7.5times10^(-5))eV^(2),JUNO can provide evidence of neutrino oscillation in the Earth at approximately the 3sigma(2sigma)level by measuring the non-zero signal rate variation with respect to the solar zenith angle.Moreover,JUNO can simultaneously measureDelta m^2_(21)using^8B solar neutrinos to a precision of 20% or better,depending on the central value,and to sub-percent precision using reactor antineutrinos.A comparison of these two measurements from the same detector will help understand the current mild inconsistency between the value of Delta m^2_(21)reported by solar neutrino experiments and the KamLAND experiment. | Angel Abusleme Thomas Adam Shakeel Ahmad Sebastiano Aiello Muhammad Akram Nawab Ali Fengpeng An Guangpeng An Qi An Giuseppe Andronico Nikolay Anfimov Vito Antonelli Tatiana Antoshkina Burin Asavapibhop João Pedro Athayde Marcondes de André Didier Auguste Andrej Babic Wander Baldini Andrea Barresi Eric Baussan Marco Bellato Antonio Bergnoli Enrico Bernieri David Biare Thilo Birkenfeld Sylvie Blin David Blum Simon Blyth Anastasia Bolshakova Mathieu Bongrand Clément Bordereau Dominique Breton Augusto Brigatti Riccardo Brugnera Riccardo Bruno Antonio Budano Max Buesken Mario Buscemi Jose Busto Ilya Butorov Anatael Cabrera Hao Cai Xiao Cai Yanke Cai Zhiyan Cai Antonio Cammi Agustin Campeny Chuanya Cao Guofu Cao Jun Cao Rossella Caruso Cédric Cerna Jinfan Chang Yun Chang Pingping Chen Po-An Chen Shaomin Chen Shenjian Chen Xurong Chen Yi-Wen Chen Yixue Chen Yu Chen Zhang Chen Jie Cheng Yaping Cheng Alexander Chepurnov Davide Chiesa Pietro Chimenti Artem Chukanov Anna Chuvashova Gérard Claverie Catia Clementi Barbara Clerbaux Selma Conforti Di Lorenzo Daniele Corti Salvatore Costa Flavio Dal Corso Christophe De La Taille Jiawei Deng Zhi Deng Ziyan Deng Wilfried Depnering Marco Diaz Xuefeng Ding Yayun Ding Bayu Dirgantara Sergey Dmitrievsky Tadeas Dohnal Georgy Donchenko Jianmeng Dong Damien Dornic Evgeny Doroshkevich Marcos Dracos Frédéric Druillole Shuxian Du Stefano Dusini Martin Dvorak Timo Enqvist Heike Enzmann Andrea Fabbri Lukas Fajt Donghua Fan Lei Fan Can Fang Jian Fang Marco Fargetta Anna Fatkina Dmitry Fedoseev Vladko Fekete Li-Cheng Feng Qichun Feng Richard Ford Andrey Formozov Amélie Fournier Haonan Gan Feng Gao Alberto Garfagnini Alexandre Göttel Christoph Genster Marco Giammarchi Agnese Giaz Nunzio Giudice Franco Giuliani Maxim Gonchar Guanghua Gong Hui Gong Oleg Gorchakov Yuri Gornushkin Marco Grassi Christian Grewing Maxim Gromov Vasily Gromov Minghao Gu Xiaofei Gu Yu Gu Mengyun Guan Nunzio Guardone Maria Gul Cong Guo Jingyuan Guo Wanlei Guo Xinheng Guo Yuhang Guo Paul Hackspacher Caren Hagner Ran Han Yang Han Miao He Wei He Tobias Heinz Patrick Hellmuth Yuekun Heng Rafael Herrera Daojin Hong YuenKeung Hor Shaojing Hou Yee Hsiung Bei-Zhen Hu Hang Hu Jianrun Hu Jun Hu Shouyang Hu Tao Hu Zhuojun Hu Chunhao Huang Guihong Huang Hanxiong Huang Qinhua Huang Wenhao Huang Xingtao Huang Yongbo Huang Jiaqi Hui Wenju Huo Cédric Huss Safeer Hussain Antonio Insolia Ara Ioannisian Daniel Ioannisyan Roberto Isocrate Kuo-Lun Jen Xiaolu Ji Xingzhao Ji Huihui Jia Junji Jia Siyu Jian Di Jiang Xiaoshan Jiang Ruyi Jin Xiaoping Jing Cécile Jollet Jari Joutsenvaara Sirichok Jungthawan Leonidas Kalousis Philipp Kampmann Li Kang Michael Karagounis Narine Kazarian Amir Khan Waseem Khan Khanchai Khosonthongkee Patrick Kinz Denis Korablev Konstantin Kouzakov Alexey Krasnoperov Svetlana Krokhaleva Zinovy Krumshteyn Andre Kruth Nikolay Kutovskiy Pasi Kuusiniemi Tobias Lachenmaier Cecilia Landini Sébastien Leblanc Frederic Lefevre Liping Lei Ruiting Lei Rupert Leitner Jason Leung Demin Li Fei Li Fule Li Haitao Li Huiling Li Jiaqi Li Jin Li Kaijie Li Mengzhao Li Nan Li Nan Li Qingjiang Li Ruhui Li Shanfeng Li Shuaijie Li Tao Li Weidong Li Weiguo Li Xiaomei Li Xiaonan Li Xinglong Li Yi Li Yufeng Li Zhibing Li Ziyuan Li Hao Liang Hao Liang Jingjing Liang Jiajun Liao Daniel Liebau Ayut Limphirat Sukit Limpijumnong Guey-Lin Lin Shengxin Lin Tao Lin Jiajie Ling Ivano Lippi Fang Liu Haidong Liu Hongbang Liu Hongjuan Liu Hongtao Liu Hu Liu Hui Liu Jianglai Liu Jinchang Liu Min Liu Qian Liu Qin Liu Runxuan Liu Shuangyu Liu Shubin Liu Shulin Liu Xiaowei Liu Yan Liu Alexey Lokhov Paolo Lombardi Claudio Lombardo Kai Loo Chuan Lu Haoqi Lu Jingbin Lu Junguang Lu Shuxiang Lu Xiaoxu Lu Bayarto Lubsandorzhiev Sultim Lubsandorzhiev Livia Ludhova Fengjiao Luo Guang Luo Pengwei Luo Shu Luo Wuming Luo Vladimir Lyashuk Qiumei Ma Si Ma Xiaoyan Ma Xubo Ma Jihane Maalmi Yury Malyshkin Fabio Mantovani Francesco Manzali Xin Mao Yajun Mao Stefano MMari Filippo Marini Sadia Marium Cristina Martellini Gisele Martin-Chassard Agnese Martini Davit Mayilyan Axel Müller Ints Mednieks Yue Meng Anselmo Meregaglia Emanuela Meroni David Meyhöfer Mauro Mezzetto Jonathan Miller Lino Miramonti Salvatore Monforte Paolo Montini Michele Montuschi Nikolay Morozov Pavithra Muralidharan Massimiliano Nastasi Dmitry VNaumov Elena Naumova Igor Nemchenok Alexey Nikolaev Feipeng Ning Zhe Ning Hiroshi Nunokawa Lothar Oberauer Juan Pedro Ochoa-Ricoux Alexander Olshevskiy Domizia Orestano Fausto Ortica Hsiao-Ru Pan Alessandro Paoloni Nina Parkalian Sergio Parmeggiano Teerapat Payupol Yatian Pei Nicomede Pelliccia Anguo Peng Haiping Peng Frédéric Perrot Pierre-Alexandre Petitjean Fabrizio Petrucci Luis Felipe Piñeres Rico Oliver Pilarczyk Artyom Popov Pascal Poussot Wathan Pratumwan Ezio Previtali Fazhi Qi Ming Qi Sen Qian Xiaohui Qian Hao Qiao Zhonghua Qin Shoukang Qiu Muhammad Rajput Gioacchino Ranucci Neill Raper Alessandra Re Henning Rebber Abdel Rebii Bin Ren Jie Ren Taras Rezinko Barbara Ricci Markus Robens Mathieu Roche Narongkiat Rodphai Aldo Romani Bedřich Roskovec Christian Roth Xiangdong Ruan Xichao Ruan Saroj Rujirawat Arseniy Rybnikov Andrey Sadovsky Paolo Saggese Giuseppe Salamanna Simone Sanfilippo Anut Sangka Nuanwan Sanguansak Utane Sawangwit Julia Sawatzki Fatma Sawy Michaela Schever Jacky Schuler Cédric Schwab Konstantin Schweizer Dmitry Selivanov Alexandr Selyunin Andrea Serafini Giulio Settanta Mariangela Settimo Muhammad Shahzad Vladislav Sharov Gang Shi Jingyan Shi Yongjiu Shi Vitaly Shutov Andrey Sidorenkov FedorŠimkovic Chiara Sirignano Jaruchit Siripak Monica Sisti Maciej Slupecki Mikhail Smirnov Oleg Smirnov Thiago Sogo-Bezerra Julanan Songwadhana Boonrucksar Soonthornthum Albert Sotnikov Ondrej Sramek Warintorn Sreethawong Achim Stahl Luca Stanco Konstantin Stankevich DušanŠtefánik Hans Steiger Jochen Steinmann Tobias Sterr Matthias Raphael Stock Virginia Strati Alexander Studenikin Gongxing Sun Shifeng Sun Xilei Sun Yongjie Sun Yongzhao Sun Narumon Suwonjandee Michal Szelezniak Jian Tang Qiang Tang Quan Tang Xiao Tang Alexander Tietzsch Igor Tkachev Tomas Tmej Konstantin Treskov Andrea Triossi Giancarlo Troni Wladyslaw Trzaska Cristina Tuve Stefan van Waasen Johannes van den Boom Guillaume Vanroyen Nikolaos Vassilopoulos Vadim Vedin Giuseppe Verde Maxim Vialkov Benoit Viaud Cristina Volpe Vit Vorobel Lucia Votano Pablo Walker Caishen Wang Chung-Hsiang Wang En Wang Guoli Wang Jian Wang Jun Wang Kunyu Wang Lu Wang Meifen Wang Meng Wang Ruiguang Wang Siguang Wang Wei Wang Wenshuai Wang Xi Wang Xiangyue Wang Yangfu Wang Yaoguang Wang Yi Wang Yifang Wang Yuanqing Wang Yuman Wang Zhe Wang Zheng Wang Zhimin Wang Zongyi Wang Apimook Watcharangkool Lianghong Wei Wei Wei Yadong Wei Liangjian Wen Christopher Wiebusch Steven Chan-Fai Wong Bjoern Wonsak Diru Wu Fangliang Wu Qun Wu Wenjie Wu Zhi Wu Michael Wurm Jacques Wurtz Christian Wysotzki Yufei Xi Dongmei Xia Yuguang Xie Zhangquan Xie Zhizhong Xing Benda Xu Donglian Xu Fanrong Xu Jilei Xu Jing Xu Meihang Xu Yin Xu Yu Xu Baojun Yan Xiongbo Yan Yupeng Yan Anbo Yang Changgen Yang Huan Yang Jie Yang Lei Yang Xiaoyu Yang Yifan Yang Haifeng Yao Zafar Yasin Jiaxuan Ye Mei Ye Ugur Yegin Frédéric Yermia Peihuai Yi Xiangwei Yin Zhengyun You Boxiang Yu Chiye Yu Chunxu Yu Hongzhao Yu Miao Yu Xianghui Yu Zeyuan Yu Chengzhuo Yuan Ying Yuan Zhenxiong Yuan Ziyi Yuan Baobiao Yue Noman Zafar Andre Zambanini Pan Zeng Shan Zeng Tingxuan Zeng Yuda Zeng Liang Zhan Feiyang Zhang Guoqing Zhang Haiqiong Zhang Honghao Zhang Jiawen Zhang Jie Zhang Jingbo Zhang Peng Zhang Qingmin Zhang Shiqi Zhang Tao Zhang Xiaomei Zhang Xuantong Zhang Yan Zhang Yinhong Zhang Yiyu Zhang Yongpeng Zhang Yuanyuan Zhang Yumei Zhang Zhenyu Zhang Zhijian Zhang Fengyi Zhao Jie Zhao Rong Zhao Shujun Zhao Tianchi Zhao Dongqin Zheng Hua Zheng Minshan Zheng Yangheng Zheng Weirong Zhong Jing Zhou Li Zhou Nan Zhou Shun Zhou Xiang Zhou Jiang Zhu Kejun Zhu Honglin Zhuang Liang Zong Jiaheng Zou | 2021 | Chinese Physics C2021，45，2： | 0 |
| 10 | Site-directed mutagenesis of long QT syndrome KCNQ1 gene in vitro显示文摘To construct a polymerase chain reaction(PCR)site-directed mutagenesis of the long QT syndrome KCNQ1 gene in vitro,two sets of primers were designed according to the sequence of KCNQ1 cDNA and a mismatch was introduced into primers.Mutagenesis was performed in a two-step PCR.The amplified fragments from the third PCR which contained the mutation site were sub-cloned into the T-vector pCR2.1.Then,the fragments containing the mutation site was obtained from pCR2.1 using restriction enzymes digestion and inserted into the same restriction site of pIRES2-EGFP-KCNQ1.The sequencing analysis shows that the mutation site was correct.Mutation from A to G in site 983 of KCNQ1 cDNA was found.Using the Effectene transfection reagent,pIRES2-EGFP-KCNQ1(G983A)was transfected into HEK cells successfully.These results may shed light on further functional study of KCNQ1 gene. | Wei LI Junguo YANG Rong DU Li TIAN Bin WANG Qiumei XU Qinmei KE Qing WANG | 2008 | Frontiers of Medicine2008，2，1： | 0 |
| 11 | Influence of Charge State on Visible Light Intensity in Collisions of Highly Charged Kr Ions with AI Surface显示文摘 | Xu Qiumei Yang Zhihu Song Zhangyong Wu Yehong Guo Yipan | 2011 | IMP & HIRFL Annual Report2011，，1： | 0 |
| 12 | 4 - 16 Ar II Spectral Measurements between 320 and 520 nm显示文摘 | Yang Zhihu Du Bin Chang Hongwei Song Zhangyong Xu Qiumei Guo Yipan | 2010 | IMP & HIRFL Annual Report2010，，1： | 0 |
| 13 | 高电荷态Xeq+离子与Ni表面作用过程中可见光发射的势能效应显示文摘When slow highly charged ions approach and enter the sample,it deposits its energy into the target surface,which leads to atomic particle eject ion.Then some of them in excited states undergo radiative de-excitation resulting in optical emission[1].In order to probe into potential energy effect on optical emission,the measurement of optical emission in the collisions of slow(V0.38 VBohr)highly charged Xeq+(4q20)ions with high purity Ni(99.995%)surface was made. | Xu Qiumei Yang Zhihu Chen Yanhong Zhao Hongyun Zhang Chonghong | 2018 | IMP & HIRFL Annual Report2018，，1： | 0 |
| 14 | Phylogenetic Analysis of Virulence Factor Genes in Salmonella from Chicken显示文摘Salmonella is a common genus of seriously harmful food-borne zoonotic bacteria. Humans and animals may be infected with Salmonella through ingestion of Salmonella-contaminated eggs and poultry meat. Therefore,in order to reduce the incidence of Salmonella infections,it is crucial to explore the pathogenic mechanism of Salmonella. invA and invE are major virulence factor genes that encode invasion proteins of Salmonella. In order to explore the pathogenic mechanism of Salmonella,phylogenetic analysis of major virulence factor genes in 33 Salmonella strains isolated from chicken was analyzed. According to the results,ivnA gene was successfully amplified from 33 Salmonella strains; ivnE gene was successfully amplified from 32 Salmonella strains. ivnA nucleotide sequences shared 72. 9%- 97. 6% homology among 12 sequenced Salmonella strains and shared 78. 9%- 97. 2% homology with those in GenB ank; ivnE nucleotide sequences shared over95. 3% homology among 23 sequenced Salmonella strains and shared 89. 6%- 98. 6% homology with those in GenB ank,which exhibited no genetic relationship to other organisms. This study provided the basis for rapid molecular detection,epidemiological research and molecular pathogenesis analysis of Salmonella. | Qiumei SHI Yanying ZHANG Xiumin WANG Baoxin YANG Zhiqiang ZHANG Tonglei WU Xiaoqiao HOU Xinhua SHAO Guoqiang ZHU | 2016 | Agricultural Biotechnology2016，5，3： | 0 |
| 15 | Optical Emission from Nickel Surface Induced by Slow Highly Charged Xe Ions显示文摘Nickel is the second most abundant transition element after iron in the sun,so the identifications of its spectral lines and electron transitions are very important for analysis of the solar spectrum. | Yang Zhiyu Xu Qiumei Chen Yanhong Zhao Hongyun | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
| 16 | Potential Energy Effect on Optical Emission from GaAs Surface Induced by Slow Highly Charged Xeq^(+)Ions显示文摘GaAs is a direct band gap semiconductor,which makes it a very useful material for the manufacture of light emitting diodes and semiconductor lasers.Ion beam modification is of potential importance for the optical and electronic device technology. | Xu Qiumei Zhang Chonghong Yang Zhihu Chen Yanhong Zhao Hongyun Song Yin | 2019 | IMP & HIRFL Annual Report2019，，1： | 0 |
| 17 | Isolation and Identification of Pathogenic Bacteria Causing Bovine Subclinical Mastitis in Eastern Hebei Province显示文摘In this study, the milk samples of 1 021 cows in eight dairy farms in Eastern Hebei Province were collected and detected with LMT reagent and somatic cell count for subclinical mastitis. Pathogenic bacteria in subclinical mastitis positive milk samples were isolated and identified.The results showed that 60.63%(619/1 021) of the sampled cows were diagnosed with subclinical mastitis, and mixed infections accounted for 88.21%(546/619) of the cases. In addition, 82 strains of 14 species were isolated from the subclinical mastitis positive milk samples, including 36 strains of Staphylococcus(43.90%), 33 strains of Streptococcus(40.24%), 8 strains of Enterobacteriaceae(9.76%) and 5 strains of Corynebacterium(6.10%), respectively. The results proved that Staphylococcus aureus and Streptococcus agalactiae are the main pathogenic bacteria causing bovine subclinical mastitis in Eastern Hebei Province. | Gao Guisheng Wu Tonglei Zhang Zhiqiang Yang Zongze Fu Zhixin Zou Fengping Gao Guangping An Jiwei Yao Weiping Shi Qiumei | 2018 | Animal Husbandry and Feed Science2018，10，1： | 0 |
| 18 | 3-17 Incident Ion Charge State Dependence of the Visible Light Emission of Xe^q+Ions Bombarding Aluminum显示文摘Slow highly charged ions(HCIs)interaction with the solid also provides information such as electron configuration,multi-interaction,ionization,excitation and charge transfer for the basic research[1??3].What is more,the understanding on the heavy HCI structure and relevant collision processes has a special meaning for inertial confinement fusion(ICF)research.Up to now,many experiments or theories in ion-solid collisions focus on the investigation of secondary electrons,sputtering ion or characteristic X-rays emission by the slow projectile[4]. | Guo Yipan Yang Zhihu Xu Qiumei Ren Jieru Zhao Hongyun Zhao Yongtao | 2016 | IMP & HIRFL Annual Report2016，，1： | 0 |
| 19 | 3-22 Visible Light Emission from GaAs Surface Bombarded by Highly Charged Xe^q+Ion显示文摘GaAs is a direct band gap semiconductor,which makes it a very useful material for the manufacture of light emitting diodes and semiconductor lasers.Impact of highly charged ions(HCI)on semiconductor solid surfaces gives rise to visible light emission from excited sputtered atoms.This work measured visible light emission from the monocrystal GaAs(100)surface bombarded by highly charged Xe^q+(6≤q≤23)as a function of charged state of the incident ion.The experiment was carried out at the 320 kV platform for multi-discipline research with HCIs in the Institute of Modern Physics,Chinese Academy of Sciences. | Xu Qiumei Yang Zhihu Chen Yanhong Zhou Xianming Zhao Hongyun Wang Yuyu Cheng Rui Lei Yu Guo Yipan | 2016 | IMP & HIRFL Annual Report2016，，1： | 0 |
| 20 | Antibiotic Sensitivity Test and Detection of Sulfonamide Resistance Gene Sul2 in Escherichia coli Isolates from Swine显示文摘[Objective] This study aimed to analyze antibiotic sensitivity and detect sulfonamide resistance gene Sul2 in 17 Escherichia coli isolates from swine.[Method]The antibiotic sensitivity of 17 E.coli isolates from swine was analyzed with Kirby-Bauer disk diffusion method.Sulfonamide resistance gene Sul2 was detected by PCR amplification with the extracted genomic DNA as a template.[Result] The 17 swine-derived E.coli isolates from different regions were resistant to various antibiotics and exhibited different multi-antibiotic resistance phenotypes,including nine isolates resistant to sulfamethoxazole,seven isolates resistant to oxacillin,ten isolates resistant to cefazolin and eight isolates resistant to erythromycin.Sulfonamide resistance gene Sul2 was successfully amplified from genomic DNA of E.coli isolates.The PCR results were basically consistent with antibiotic resistance phenotypes of these strains.[Conclusion] Sul2 gene was widespread in swine-derived E.coli,which was closely associated with sulfonamide resistance phenotypes of E.coli. | Yanying ZHANG Fang XIANG Rui GUO Zhaoxing ZHANG Qiumei SHI Yuqin LIU Cairan YANG Zongze YANG Guangping GAO Guisheng GAO Zhi PENG Xun SUO | 2016 | Agricultural Biotechnology2016，5，2： | 0 |
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