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| 1 | Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils,Lissorhoptrus oryzophilus Kuschel(Coleoptera:Curculionidae)显示文摘The rice water weevil,Lissorhoptrus oryzophilus Kuschel(Coleoptera:Curculionidae),reproduces by sex in the Southeastern United States,but reproduces by parthenogenesis in California and other invaded regions in Asia and Europe.The objective of this study was to create a parthenogenetic gene expression profile of the rice water weevil in order to gain a better insight into the molecular mechanisms of parthenogenesis in the weevil.Suppression subtractive hybridization(SSH) technique was employed for profiling differential gene expression in the developed ovary between the parthenogenetic and bisexual female rice water weevils.A total of 70 contigs were obtained,and the BLASTX search identified putatively 28 genes with differential functions.According to the cytological process of parthenogenesis,the tubulin alpha-1 chain and signal transduction genes etc.were selected for real time quantitative RT-PCR analyses,and their possible functions related to the molecular mechanism of parthenogenesis were discussed.The tubulin alpha-1 chain and some signal transduction genes may be related to the molecular mechanisms of parthenogenesis of the rice water weevil. | YANG Pu ZHOU WenWu ZHANG Qin CHENG JiaAn ZHU ZengRongx WAY MO | 2009 | Chinese Science Bulletin2009，54，20： | 8 |
| 2 | A new distinct geminivirus causes soybean stay-green disease显示文摘Dear Editor,Plant viruses make up almost half of the plant disease-causing pathogens,affecting crop yields and the global economy(Savary et al.,2019).Soybean(Glycine max)is one of the most valuable legume crops in the world,supplying 25%of the global edible oil and two-thirds of the global concentrated protein for livestock feeding.Recently,the outbreak of soybean stay-green syndrome with delayed leaf senescence(stay-green),flat pods,and increased number of abnormal seeds has swept the soybean production in the Huang-Huai-Hai region of China,resulting in huge yield losses(Xu et al.,2019).This disease has become an epidemic and prominent problem in soybean production and is still expanding its geography,including North America,posing a serious threat to soybean production(Harbach et al.,2016;Zhang et al.,2016;Li et al.,2019).However,the cause of soybean stay-green syndrome remains obscure. | Ruixiang Cheng Ruoxin Mei Rong Yan Hongyu Chen Dan Miao Lina Cai Jiayi Fan Gairu Li Ran Xu Weiguo Lu Yu Gao Wenwu Ye Shuo Su Tianfu Han Junyi Gai Yuanchao Wang Xiaorong Tao Yi Xu | 2022 | Molecular Plant2022，15，6： | 6 |
| 3 | Baicalein suppresses vasculogenic mimicry through inhibiting RhoA/ROCK expression in lung cancer A549 cell line显示文摘Vasculogenic mimicry(VM)refers to a new tubular network of the blood supply system with abundant extracellular matrix.VM is similar to capillaries but does not involve endothelial cells.As a traditional herbal medicine commonly used in China,baicalein possesses anti-inflammatory and lipoxygenase activities.However,the effects of baicalein on the process of VM formation in nonsmall cell lung cancer(NSCLC)and the underlying mechanisms have remained poorly understood.In this study,baicalein was found to inhibit the viability and motility of A549 cells and induced the breakage of the cytoskeletal actin filament network.In addition,baicalein significantly decreased the formation of VM and downregulated the expressions of VM-associated factors,such as VEcadherin,EphA2,MMP14,MMP2,MIVIP9,PI3K and LAMC2,similar to the effects of ROCK inhibitors.In deed,baicalei n inhibited RhoA/ROCK expression in vitro and in vivo,suggesting the underlying mechanisms of reduced VM formation.Collectively,baicalein suppressed the formation of VM in NSCLC by targeting the RhoA/ROCK signaling pathway,indicating that baicalein might serve as an emerging drug for NSCLC. | Zhe Zhang Li Nong Menglei Chen Xiaoli Gu Weiwei Zhao Minghui Liu Wenwu Cheng | 2020 | Acta Biochimica et Biophysica Sinica2020，52，9： | 5 |
| 4 | The self-regulation process and its mechanism of channels' bed changes in the Changjiang(Yangtze) Estuary in China显示文摘Recent bathymetric changes in the Changjiang Estuary under the influence of artificial regulation engineerings and basin reservoirs have been analyzed based on the maritime charts since 1997 and recent fieldworks. The results indicate a slight erosion of the channels in the upper and middle estuary, continuing deposition and seaward move of the mouth bar crest and intensifying erosion at the nearshore seabed. It is noteworthy that the morphological evolution caused by intensive human activities dominates over the changes from nature process.First, the riverbes are eroded overall in the South Branch(SB), the South Channel(SC) and the upper and middle reaches of the North Channel(NC). The nearshore seabed outside the river mouth is being eroded slightly, which is attributed to the declining sediment supply from the Changjiang Basin due to the construction of the Three Gorges Dam upstream. The sediment above the seabed is very active and coarsened, meanwhile, sand waves are becoming more distinct. Second, a deposition occurs in the North Brach(NB), the mouth of the NC, the mouth bars of the North Passage(NP) and the South Passage(SP) and especially the main channel of the NP, where it shows a massive siltation after the deep waterway project. The reasons for the recent changes are not only the dynamic structure in estuarine mouth bars, but also the supply of sediment resuspension in a local and offshore area. Meanwhile, the severe erosion and siltation in some reaches is related to the construction of estuarine engineerings. It is indicated that the Changjiang Estuary is gradually self-adjusted and adapting to the varying natural factors and intensive human activities. The study on the mechanism of self-regulation of the recent bathymetric changes in the Changjiang Estuary has important and practical significance. | ZHANG Xiaohe LI Jiufa ZHU Wenwu CHENG Heqin CHEN Wei | 2015 | Acta Oceanologica Sinica2015，34，7： | 5 |
| 5 | Sulforaphane protects brains against hypoxic–ischemic injury through induction of Nrf2-dependent phase 2 enzyme显示文摘 | Zhang Ping Wenwu Liu Zhimin Kang Jianmei Cai Qiusha Wang Ni Cheng Sujian Wang Shizhong Wang John H. Zhang Xuejun Sun | 2010 | Brain Research2010，，： | 1 |
| 6 | Field Control Effects of Four Biopesticides on Grape Gray Mold显示文摘[Objectives]The paper was to search for biopesticides with good control effect on grape gray mold.[Methods]A randomized field trial was carried out to compare the control effects of 100 million CFU/g Trichoderma harzianum WDG,200 million spores/g Trichoderma WP,24% validamycin AS,0.3% matrine AS on grape gray mold.[Results]Of the 4 biopesticides,200 million spores/g Trichoderma WP had the highest control effect on grape gray mold,with the control effect of 73.38% at 7 d post the second application,significantly higher than those of 100 million CFU/g T.harzianum WG and 0.3% matrine AS;the control effect was 75.77% at 14 d post the second application,significantly higher than those of other 3 biopesticides.[Conclusions]The 200 million spores/g Trichoderma WP shows a good control effect on grape gray mold with a long-lasting control effect,so it is more is for the control of summer black grape gray mold in Jianshui County. | Rui MA Jianbin WEN Wenwu SHEN Hongyan ZHAN Jiahua XIE Mao CHENG | 2022 | Plant Diseases and Pests2022，13，3： | 0 |
| 7 | Transcription factor CsTT8 promotes fruit coloration by positively regulating the methylerythritol 4-phosphate pathway and carotenoid biosynthesis pathway in citrus (Citrus spp.)显示文摘Carotenoids directly influence citrus fruit color and nutritional value,which is critical to consumer acceptance.Elucidating the potential molecular mechanism underlying carotenoid metabolism is of great importance for improving fruit quality.Despite the well-established carotenoid biosynthetic pathways,the molecular regulatory mechanism underlying carotenoid metabolism remains poorly understood.Our previous studies have reported that the Myc-type basic helix-loop-helix(bHLH)transcription factor(TF)regulates citrus proanthocyanidin biosynthesis.Transgenic analyses further showed that overexpression of CsTT8 could significantly promote carotenoid accumulation in transgenic citrus calli,but its regulatory mechanism is still unclear.In the present study,we found that overexpression of CsTT8 enhances carotenoid content in citrus fruit and calli by increasing the expression of CsDXR,CsHDS,CsHDR,CsPDS,CsLCYE,CsZEP,and CsNCED2,which was accompanied by changes in the contents of abscisic acid and gibberellin.The in vitro and in vivo assays indicated that CsTT8 directly bound to the promoters of CsDXR,CsHDS,and CsHDR,the keymetabolic enzymes of the methylerythritol 4-phosphate(MEP)pathway,thus providing precursors for carotenoid biosynthesis and transcriptionally activating the expression of these three genes.In addition,CsTT8 activated the promoters of four key carotenoid biosynthesis pathway genes,CsPDS,CsLCYE,CsZEP,and CsNCED2,directly promoting carotenoid biosynthesis.This study reveals a novel network of carotenoid metabolism regulated by CsTT8.Our findings will contribute to manipulating carotenoid metabolic engineering to improve the quality of citrus fruit and other crops. | Quan Sun Zhengchen He Ranran Wei Yingzi Yin Junli Ye Lijun Chai Zongzhou Xie Wenwu Guo Juan Xu Yunjiang Cheng Qiang Xu Xiuxin Deng | 2023 | Horticulture Research2023，10，11： | 0 |
| 8 | DeepTSP:Deep traffic state prediction model based on large-scale empirical data显示文摘Real-time traffic state(e.g.,speed)prediction is an essential component for traffic control and management in an urban road network.How to build an effective large-scale traffic state prediction system is a challenging but highly valuable problem.This study focuses on the construction of an effective solution designed for spatiotemporal data to predict the traffic state of large-scale traffic systems.In this study,we first summarize the three challenges faced by large-scale traffic state prediction,i.e.,scale,granularity,and sparsity.Based on the domain knowledge of traffic engineering,the propagation of traffic states along the road network is theoretically analyzed,which are elaborated in aspects of the temporal and spatial propagation of traffic state,traffic state experience replay,and multi-source data fusion.A deep learning architecture,termed as Deep Traffic State Prediction(DeepTSP),is therefore proposed to address the current challenges in traffic state prediction.Experiments demonstrate that the proposed DeepTSP model can effectively predict large-scale traffic states. | Yang Liu Cheng Lyu Yuan Zhang Zhiyuan Liu Wenwu Yu Xiaobo Qu | 2021 | Communications in Transportation Research2021，1，1： | 0 |
| 9 | DICE方案与CHOP方案治疗中高度恶性非霍奇金淋巴瘤的随机对照研究(英文)显示文摘Objective: To compare efficacies and safeties of DICE and CHOP regimens in treating intermediate and high grade non-Hodgkin's lymphoma (NHL), and indicate the standard treatment for it. Methods: A total of 74 patients with moder- ately or highly malignant NHL, verified by pathology or histology, were randomized into the trial group (37 patients treated with DICE regimen) and the control group (37 patients treated with CHOP regimen). Survival rate was analyzed by Kaplan-Meier method. Chi-square test was performed between groups. Results: The complete response rate, partial response rate, and response rate were significantly higher in DICE group than in CHOP group (40.5% vs. 29.7%, 37.8% vs. 27.0%, and 78.3% vs. 56.7%, respectively, P < 0.05). The 1-, 3-, and 5-year survival rates were significantly higher in DICE group than in CHOP group (89.2% vs. 81.2%, 76.0% vs. 52.6%%, and 46.7% vs. 36.4%, respectively, P < 0.05). The major side effects, appeared with no differences (P > 0.05) in incidences between the two groups, were leukopenia, thrombocytopenia, and nausea. There were only three episodes of clinical cystitis or gross haematuria in DICE regimen. Conclusion: The results showed higher efficacy of DICE regimen over CHOP regimen. DICE regimen may prolong the survival time of patients with moderately and highly malignant NHL. | Wenwu Wang Xuenong OuYang Zhangshu Cheng Yonghai Peng Fangwei Xie Zongyang Yu | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，2： | 0 |
| 10 | Illuminating the cells:transient transformation of citrus to study gene functions and organelle activities related to fruit quality显示文摘Although multiple microscopic techniques have been applied to horticultural research,few studies of individual organelles in living fruit cells have been reported to date.In this paper,we established an efficient system for the transient transformation of citrus fruits using an Agrobacterium-mediated method.Kumquat(Fortunella crassifolia Swingle)was used;it exhibits higher transformation efficiency than all citrus fruits that have been tested and a prolonged-expression window.Fruits were transformed with fluorescent reporters,and confocal microscopy and live-cell imaging were used to study their localization and dynamics.Moreover,various pH sensors targeting different subcellular compartments were expressed,and the local pH environments in cells from different plant tissues were compared.The results indicated that vacuoles are most likely the main organelles that contribute to the low pH of citrus fruits.In summary,our method is effective for studying various membrane trafficking events,protein localization,and cell physiology in fruit and can provide new insight into fruit biology research. | Jinli Gong Zhen Tian Xiaolu Qu Qiunan Meng Yajie Guan Ping Liu Chuanwu Chen Xiuxin Deng Wenwu Guo Yunjiang Cheng Pengwei Wang | 2021 | Horticulture Research2021，8，1： | 0 |
| 11 | CACA Guidelines for Holistic Integrative Management of Breast Cancer显示文摘Purpose:Breast cancer is now the most common malignant tumor worldwide.About one-fourth of female cancer patients all over the world sufer from breast cancer.And about one in six female cancer deaths worldwide is caused by breast cancer.In terms of absolute numbers of cases and deaths,China ranks frst in the world.The CACA Guidelines for Holistic Integrative Management of Breast Cancer were edited to help improve the diagnosis and comprehensive treatment in China.Methods:The Grading of Recommendations Assessment,Development and Evaluation(GRADE)was used to classify evidence and consensus.Results:The CACA Guidelines for Holistic Integrative Management of Breast Cancer include the epidemiology of breast cancer,breast cancer screening,breast cancer diagnosis,early breast cancer treatment,advanced breast cancer treatment,follow-up,rehabilitation,and traditional Chinese medicine treatment of breast cancer patients.Conclusion:We to standardize the diagnosis and treatment of breast cancer in China through the formulation of the CACA Guidelines. | Jiong Wu Daiming Fan Zhimin Shao Binghe Xu Guosheng Ren Zefei Jiang Yongsheng Wang Feng Jin Jin Zhang Qingyuan Zhang Fei Ma Jinli Ma Zhonghua Wang Shusen Wang Xiaojia Wang Shu Wang Haibo Wang Tao Wang Xiang Wang Jing Wang Jia Wang Biyun Wang Li Fu Hongyuan Li Yehui Shi Lu Gan Yunjiang Liu Jian Liu Zhenzhen Liu Qiang Liu Qiang Sun Wenwu Cheng Keda Yu Zhongsheng Tong Xinhong Wu Chuangui Song Jianguo Zhang Jian Zhang Junjie Li Bin Li Man Li Huiping Li Wentao Yang Hongjian Yang Benlong Yang Hong Bu Juping Shen Zhenzhou Shen Yiding Chen Ceshi Chen Da Pang Zhimin Fan Ying Zheng Xiaoli Yu Guangyu Liu Xichun Hu Yiqun Ling Jinhai Tang Yongmei Yin Cuizhi Geng Peng Yuan Yajia Gu Cai Chang Xuchen Cao Yuan Sheng Yuanxi Huang Jian Huang Weijun Peng Xiaohua Zeng Yuntao Xie Ning Liao Committee of Breast Cancer Society,Chinese Anti-Cancer Association | 2022 | Holistic Integrative Oncology2022，1，1： | 0 |
| 12 | Value of Traditional Chinese Medicine syndrome differentiation in predicting the survival time of patients with advanced cancer显示文摘OBJECTIVE:To prospectively study the accuracy of the palliative prognostic index(PPI)survival prediction model combined with Traditional Chinese Medicine(TCM)syndrome differentiation.METHODS:The PPI survival prediction model was used to predict survival time.Patients'real survival time was recorded.The survival time was calculated using the Kaplan-Meier method,and the logrank method was used to test the difference.RESULTS:The average PPI survival prediction score of 227 patients was 5.83(95%CI:5.29-6.37).There was a significant difference in the real-life period between the different PPI groups(P<0.05).PPI group Ⅰ(predicted survival of >6 weeks)showed the highest predictive sensitivity and PPI group Ⅱ(predicted survival of 3-6 weeks)showed the highest predictive specificity.According to TCM syndrome differentiation,82 cases(36%)were diagnosed with liver and kidney Yin deficiency(type Ⅳ).The actual survival time of type Ⅳ patients was significantly shorter than that of other types of patients(mean:21.85 vs 28.70,P=0.007).In group Ⅰ,the median survival time of type Ⅳ patients and other types was 25 and 34 d,respectively(P<0.001).The sensitivity and specificity of PPI prediction were improved in group Ⅱ by TCM syndrome differentiation.For patients in group Ⅲ whose predicted survival time was<3 weeks,the specificity of PPI survival prediction was higher in type Ⅳ patients.CONCLUSION:This study shows that the PPI predictive tool for survival rate has important value.TCM syndrome differentiation and typing has certain significance for further classification and survival prediction. | GU Xiaoli CHEN Menglei LIU Minghui ZHANG Zhe ZHAO Weiwei CHENG Wenwu | 2021 | Journal of Traditional Chinese Medicine2021，41，4： | 0 |
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