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| 1 | Characterization and biological significance of IL-23-induced neutrophil polarization显示文摘Neutrophils are heterogeneous with distinct subsets,and can switch phenotypes to exert regulatory functions on immunity.We herein demonstrate that IL-23-treated neutrophils selectively produce IL-17A,IL-17F and IL-22,and display a distinct gene expression profile in contrast to resting and lipopolysaccharide-treated neutrophils.IL-17+neutrophils are present in the colons of mice with dextran sulfate sodium-induced colitis.Adoptive transfer of IL-23-treated neutrophils significantly promotes pathogenesis in this model.IL-23 induces neutrophil polarization through STAT3-dependent RORγt and BATF pathways.Thus,IL-23-induced neutrophil polarization expresses a unique cytokine-producing profile,which may contribute to IL-23-mediated inflammatory diseases. | Yang Li Linnan Zhu Zhulang Chu Tao Yang Hai-Xi Sun Fan Yang Wei Wang Yuzhu Hou Peng Wang Qingjie Zhao Yaling Tao Lianfeng Zhang Xiaodong Zhang Yong Zhao | 2018 | Cellular & Molecular Immunology2018，15，5： | 3 |
| 2 | Arbitrary polygonal 2D finite elements 显示文摘 | Fan Yaling Zhang Gaoyuan Lu Mingwan | 1995 | Chinese Journal of Mechanics1995，27，6： | 1 |
| 3 | Evolution of drug regulations and regulatory innovation for anticancer drugs in China显示文摘Over the past two decades,China has introduced significant changes to drug regulations through regulatory innovations to accelerate drug review and approvals,keeping in line with the rapidly growing scientific innovation in drug research and development(R&D).In this study,we outlined the revolution of drug regulation in China since the establishment of the State Drug Administration in 1998.More particularly,we performed a comprehensive analysis of newly approved anticancer drugs in China from the year 2005 to May 2021,as a powerful illustration of how the revolution has changed the drug R&D landscape.Innovative drug development in China has boomed,benefiting in particular from pro-innovation policies as well as expedited program designations by the authority.We found a significant increase in the number of both imported and domestic new anticancer drugs from 2005 to 2021,with the emergence of drugs with novel mechanisms of action,including immune checkpoint inhibitors and cell therapy products.Drug lag has also been dramatically shortened by more than 70%for imported drugs in years 2016-2020 compared to years 2006-2010.Furthermore,we provide an insight into the potential approaches to further optimize the science-based and clinical value-based regulatory and R&D drug ecosystem in China.This review provides evidence of significant impacts of regulations and policies on drug R&D and suggests that the constantly adapting regulatory ecosystem will speed up drug development in China and worldwide. | Yang Liu Ning Zhang Cuicui Xie Yale Jiang Yunhe Qin Liyun Zhou Yi Fan Lianjie Ren Chen Yin Huan Yang Wei Xie Qing Zhai Guanqiao Li Hongzhuan Chen Xiaoyuan Chen | 2022 | Acta Pharmaceutica Sinica B2022，12，12： | 1 |
| 4 | A direct,sensitive and high-throughput genus and species-specific molecular assay for large-scale malaria screening显示文摘Background:Infectious disease diagnostics often requires sensitive molecular assays that identify at both genus and species levels.For large scale screening,such as malaria screening for elimination,diagnostic assay can be a challenge,as both the throughput and cost of the assay must be considered.The requirement of nucleic acid extraction hampers the throughput of most molecular assays.Co-amplification of multiple species or multiplex identification either can result in missed diagnosis or are too costly for large-scale screening.A genus-and species-specific diagnostic assay with simplified procedure,high sensitivity and throughput is still needed.This study aimed to develop a sensitive and high-throughput approach for large-scale infectious disease screening.Methods:We developed multi-section Capture and Ligation Probe PCR(mCLIP-PCR)for the direct detection of RNA without extraction and reverse transcription.Multiple tailed sandwich hybridization probes were used to bind at genus-and species-specific sections of the target RNA to cooperatively capture the target onto a 96-well plate.After enzymatic ligation of the bound probes,a single-stranded DNA formed at each section with distinct tail sequence at the ends.They were separately PCR-amplified with primers corresponding to tail sequences for genus or species identification.We applied the method to the active screening ofPlasmodium infections of 4,580 asymptomatic dried blood spot samples collected in malaria endemic areas and compared the results with standard qPCR using linear regression.Results:With multi-section cooperative capture but separate amplification strategy,we accurately identified genusPlasmodium and speciesP.falciparum andP.vivax without RNA extraction,with favorable sensitivities among the published reports.In the active screening,our method identified all 53 positive infections including two mixed infections,and twoP.vivax infections that were missed by standard qPCR.Conclusions:mCLIP-PCR provides a sensitive and high-throughput approach to large-scale infectious disease screening with low cost and labor,making it a valuable tool for malaria elimination in endemic region. | Yaling Zhao Ye Zhao Yu Sun Lihua Fan Duoquan Wang Heng Wang Xiaodong Sun Zhi Zheng | 2022 | Infectious Diseases of Poverty2022，11，2： | 0 |
| 5 | COVID-19 induces new-onset insulin resistance and lipid metabolic dysregulation via regulation of secreted metabolic factors显示文摘Abnormal glucose and lipid metabolism in COVID-19 patients were recently reported with unclear mechanism.In this study,we retrospectively investigated a cohort of COVID-19 patients without pre-existing metabolic-related diseases,and found new-onset in suli n resista nee,hyperglycemia,and decreased HDL-C in these patie nts.Mecha nistically,SARS-CoV-2 infecti on in creased the expression of RE1-silencing transcription factor(REST),which modulated the expression of secreted metabolic factors including myeloperoxidase,apelin,and myostatin at the transcriptional level,resulting in the perturbation of glucose and lipid metabolism.Furthermore,several lipids,including(±)5-HETE,(±)12-HETE,propionic acid,and isobutyric acid were identified as the potential biomarkers of COVID-19-induced metabolic dysregulation,especially in insulin resistance.Taken together,our study revealed insulin resistance as the direct cause of hyperglycemia upon COVID-19,and further illustrated the underlying mechanisms,providing potential therapeutic targets for COVID-19-induced metabolic complications. | Xi He Chenshu Liu Jiangyun Peng Zilun Li Fang Li Jian Wang Ao Hu Meixiu Peng Kan Huang Dongxiao Fan Na Li Fuchun Zhang Weiping Cai Xinghua Tan Zhongwei Hu Xilong Deng Yueping Li Xiaoneng Mo Linghua Li Yaling Shi Li Yang Yuanyuan Zhu Yanrong Wu Huichao Liang Baolin Liao Wenxin Hong Ruiying He Jiaojiao Li Pengle Guo Youguang Zhuo Lingzhai Zhao Fengyu Hu Wenxue Li Wei Zhu Zefeng Zhang Zeling Guo Wei Zhang Xiqiang Hong Wei kang Cai Lei Gu Ziming Du Yang Zhang Jin Xu Tao Zuo Kai Deng Li Yan Xinwen Chen Sifan Chen Chunliang Lei | 2022 | Signal Transduction and Targeted Therapy2022，7，1： | 0 |
| 6 | Rare tumors: a blue ocean of investigation显示文摘Advances in novel drugs,therapies,and genetic techniques have revolutionized the diagnosis and treatment of cancers,substantially improving cancer patients’prognosis.Although rare tumors account for a non-negligible number,the practice of precision medicine and development of novel therapies are largely hampered by many obstacles.Their low incidence and drastic regional disparities result in the difficulty of informative evidence-based diagnosis and subtyping.Sample exhaustion due to difficulty in diagnosis also leads to a lack of recommended therapeutic strategies in clinical guidelines,insufficient biomarkers for prognosis/efficacy,and inability to identify potential novel therapies in clinical trials.Herein,by reviewing the epidemiological data of Chinese solid tumors and publications defining rare tumors in other areas,we proposed a definition of rare tumor in China,including 515 tumor types with incidences of less than 2.5/100000 per year.We also summarized the current diagnosis process,treatment recommendations,and global developmental progress of targeted drugs and immunotherapy agents on the status quo.Lastly,we pinpointed the current recommendation chance for patients with rare tumors to be involved in a clinical trial by NCCN.With this informative report,we aimed to raise awareness on the importance of rare tumor investigations and guarantee a bright future for rare tumor patients. | Shuhang Wang Peiwen Ma Ning Jiang Yale Jiang Yue Yu Yuan Fang Huilei Miao Huiyao Huang Qiyu Tang Dandan Cui Hong Fang Huishan Zhang Qi Fan Yuning Wang Gang Liu Zicheng Yu Qi Lei Ning Li | 2023 | Frontiers of Medicine2023，17，2： | 0 |
| 7 | Insights into varicella-zoster virus assembly from the B-and C-capsid at near-atomic resolution structures显示文摘Varicella-zoster is a highly communicable virus that can be transmitted through the airborne route.About one quarter of people are infected with this virus.Previous studies have described the structure of A-capsid and a blurred reconstruction of the C-capsid with icosahedral symmetry.In this study,we have determined the more precise detailed structures of the varicella-zoster virus(VZV)B-and C-capsid in icosahedral symmetry using a combination of block-based reconstruction and symmetry relaxation strategies.In addition,we are reporting structural details of the portal vertex reconstructions in five-fold symmetry and portal reconstructions in twelve-fold symmetry.The structures unveil the basis for the high thermal stability of the VZV capsid.The conformational flexibility of structural elements of the capsid plays a role in the assembly of the capsid and drives processes critical for the viral life cycle.The results of the study open up new avenues for the development of drugs against a highly prevalent and contagious pathogen. | Lei Cao Nan Wang Zhe Lv Wenyuan Chen Zhonghao Chen Lifei Song Xueyan Sha Guiqiang Wang Yaling Hu Xiaojun Lian Guoliang Cui Jinyan Fan Yaru Quan Hongrong Liu Hai Hou Xiangxi Wang | 2024 | hLife2024，2，2： | 0 |
| 8 | Performance of Hepatitis Delta Virus(HDV)RNA Testing for the Diagnosis of Active HDV Infection:Systematic Review and Meta-analysis显示文摘Background and Aims:Hepatitis delta virus(HDV)is a defective virus and causes severe liver disease.Several HDV RNA assays have been developed,however the diagnostic efficacy remains unclear.This systematic review and metaanalysis aims to evaluate the diagnostic accuracy of HDV RNA assays to aid in the diagnosis of active hepatitis D.Methods:The PubMed,Embase,and Cochrane Library databases were systematically searched from the beginning to June 31,2022.Information on the characteristics of the literature and data on sensitivity,specificity,and area under curve(AUC)of the receiver operating characteristic(ROC)were extracted.Stata 14.0 was used for meta-analysis of the combined sensitivity,specificity,positive likelihood ratio,and negative likelihood ratio.Results:A total of 10 studies were included in the meta-analysis.The summary sensitivity,specificity,positive likelihood ratio,negative likelihood ratio,and diagnostic odds ratio of HDV RNA assays for HDV diagnosis were 0.92(95%CI:0.87-0.95),0.90(95%CI:0.86-0.93),7.74(95%CI:5.31-11.29),0.10(95%CI:0.06-0.18)and 99.90(95%CI:47.08-211.99),respectively.The AUC of the pooled ROC curve was 0.95(95%CI:0.92-0.96).Conclusions:The results show that HDV RNA assays had high diagnostic performance.However,that is limited by the number and quality of studies.Standard protocols for the development of assays by manufacturers and larger studies on the use of the assays are needed. | Sisi Chen Xiangying Zhang Ling Xu Yuan Tian Zihao Fan Yaling Cao Zhenzhen Pan Yao Gao Sujun Zheng Zhongping Duan Mei Liu Feng Ren | 2023 | Journal of Clinical and Translational Hepatology2023，11，6： | 0 |
| 9 | Fabrication and luminescence of highly transparent C12A7:Tb^(3+)glass-ceramics via in-situ crystallization from aerodynamic levitation processed glasses显示文摘Rare earth doped 12CaO·7Al_(2)O_(3)(C12A7)glass-ceramics are one of attractive photonic materials due to their high transparency,high luminescent efficiency and eco-friendly nature.A series of C12A7 glasses with different Tb^(3+)doping concentrations was prepared by aerodynamic levitation method.The C12A7:Tb glass-ceramics were obtained via in-situ growth from the glasses with the identical chemical composition at 800℃.The microstructure observation demonstrates that the elemental distributions of Ca and Al are homogeneous in the glass-ceramics and the grain size of C12A7 is 4-70 nm.The in-line transmittance of glass-ceramics reaches as high as 82%at 1500 nm.The emission spectra of the C12A7:Tb glass-ceramics present characteristic Tb^(3+)emission peaks at 486 nm(^(5)D_(4)→^(7)F_6),541 nm(^(5)D_(4)→^(7)F_(5)),585 nm(^(5)D_(4)→^(7)F_(4))and 621 nm(^(5)D_(4)→^(7)F_(3)).The photolumine scence lifetime of C12A7:0.3%Tb glass-ceramic is 1.9 ms.Compared with the emission intensity of the glasses,that of the glass-ceramics increases due to the reducing in concentration of oxygen vacancy associated with network forming(NWF)cations.The highly transparent C12A7:Tb glass-ceramics may be a promising candidate for green light source in solid state lighting. | Yaling Zhang Lingcong Fan Xuefeng Wang Jiansheng Xie Ying Shi | 2023 | Journal of Rare Earths2023，41，11： | 0 |
| 10 | Formation of multifaceted nano-groove structure on rutile TiO_(2) photoanode for efficient electron-hole separation and water splitting显示文摘Photoelectrochemical(PEC)water-splitting using solar energy holds great promise for the renewable energy future,and a key challenge in the development of industry viable PEC devices is the unavailability of high-efficient photoanodes.Herein,we designed a TiO_(2) model photocatalyst with nano-groove pattern and different surface orientation using low-energy Ar+irradiation and photoetching of TiO_(2),and significantly improved the intrinsic activity for PEC water oxidation.High-resolution transmission electron microscopy directly manifests that the grooves consist of highly stepped surface with<110>steps and well-crystallized.Transient absorption spectroscopy reveals the groove surface that allows for increased recovery lifetime,which ensures promoted electron-hole separation efficiency.Surface photovoltage directly shows the carrier separation and transportation behaviors,verified by selective photodeposition,demonstrating the groove surface on TiO_(2) contributes to electron-hole separation.This work proposes an efficient and scalable photoanode strategy,which potentially can open new opportunities for achieving efficient PEC water oxidation performance. | Xiaoyi Zhan Yaling Luo Ziyu Wang Yao Xiang Zheng Peng Yong Han Hui Zhang Ruotian Chen Qin Zhou Hongru Peng Hao Huang Weimin Liu Xin Ou Guijun Ma Fengtao Fan Fan Yang Can Li Zhi Liu | 2022 | Journal of Energy Chemistry2022，31，2： | 0 |
| 11 | A[Th_(8)Co_(8)]Nanocage-Based Metal–Organic Framework with Extremely Narrow Window but Flexible Nature Enabling Dual-Sieving Effect for Both Isotope and Isomer Separation显示文摘The synthesis of nanoporous materials that display a combination of molecular sieving(MS)and quantum sieving(QS)effects is still a challenging task.In this work,we have demonstrated the synthesis of a nanocaged metal–organic framework(MOF),ECUT-8,that has a dual-sieving capability.ECUT-8 afforded H_(2)/D_(2) isotope separation due to its extremely narrow window size(3.0Å),resulting in QS.Further,the framework flexibility of ECUT-8 was exploited for the separation of butane and hexane isomers due to its MS effect.Other desirable features of ECUT-8 include high thermal,water,and chemical stability,making it suitable for practical application.Herein,these results open up an avenue to design the effects of coexistence of multiple sieving in one material. | Mengjia Yin Rajamani Krishna Wenjing Wang Daqiang Yuan Yaling Fan Xuefeng Feng Li Wang Feng Luo | 2022 | CCS Chemistry2022，4，3： | 0 |
| 12 | Immunogenic cell death effects induced by doxorubicin improved chemo-immunotherapy via restoration of granzyme B activity显示文摘Chemotherapy remains one of the irreplaceable treatments for cancer therapy.The use of immunogenic cell death(ICD)-inducing chemotherapeutic drugs offers a practical strategy for killing cancer cells,simultaneously eliciting an antitumor immune response by promoting the recruitment of cytotoxic immune cells and production of granzyme B(GrB).However,numerous malignant cancers adaptively acquired the capacity of secreting serpinb9(Sb9),a physiological inhibitor of GrB,which can reversibly inhibit the biological activity of GrB.To circumvent this dilemma,in this study,an integrated tailor-made nanomedicine composed of tumor-targeting peptide(Arg-Gly-Asp,RGD)decorated liposome,doxorubicin(DOX,an effective ICD inducer),and the compound 3034(an inhibitor of Sb9),is developed(termed as D3RL)for breast cancer chemo-immunotherapy.In vitro and in vivo studies show that D3RL can directly kill tumor cells and trigger the host immune response by inducing ICD.Meanwhile,D3RL can competitively relieve the inhibition of Sb9 to GrB.The restored GrB can not only effectively induce tumor immunotherapy,but also degrade matrix components in the tumor microenvironment,consequently improving the infiltration of immune cells and the penetration of nanomedicines,which in return enhance the combined antitumor effect.Taken together,this work develops an integrated therapeutic solution for targeted production and restoration of GrB to achieve a combined chemo-immunotherapy for breast cancer. | Tao Huang Xiaofan Sun Yingqiu Qi Xi Yang Linyao Fan Mengdie Chen Yale Yue Hong Ge Yiye Li Guangjun Nie Huan Min Xianfu Sun | 2023 | Nano Research2023，16，12： | 0 |
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