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257篇 您的检索式:作者名="Dan Qi"
    题名 作者 年代 出处 被引量
1Enhanced secondary pollution offset reduction of primary emissions during COVID-19 lockdown in China显示文摘To control the spread of the 2019 novel coronavirus(COVID-19),China imposed nationwide restrictions on the movement of its population(lockdown)after the Chinese New Year of 2020,leading to large reductions in economic activities and associated emissions.Despite such large decreases in primary pollution,there were nonetheless several periods of heavy haze pollution in eastern China,raising questions about the well-established relationship between human activities and air quality.Here,using comprehensive measurements and modeling,we show that the haze during the COVID lockdown was driven by enhancements of secondary pollution.In particular,large decreases in NOx emissions from transportation increased ozone and nighttime NO_(3)radical formation,and these increases in atmospheric oxidizing capacity in turn facilitated the formation of secondary particulate matter.Our results,afforded by the tragic natural experiment of the COVID-19 pandemic,indicate that haze mitigation depends upon a coordinated and balanced strategy for controlling multiple pollutants.Xin Huang Aijun Ding Jian Gao Bo Zheng Derong Zhou Ximeng Qi Rong Tang Jiaping Wang Chuanhua Ren Wei Nie Xuguang Chi Zheng Xu Liangduo Chen Yuanyuan Li Fei Che Nini Pang Haikun Wang Dan Tong Wei Qin Wei Cheng Weijing Liu Qingyan Fu Baoxian Liu Fahe Chai Steven JDavis Qiang Zhang Kebin He 2021National Science Review2021,8,2:47
2Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li 2015Cell Research2015,25,1:37
3Effects of glycyrrhetinic acid on collagen metabolism of hepatic stellate cells at different stages of liver fibrosis in rats显示文摘INTRODUCTIONLiver fibrosis is a dynamic course leading tocirrhosis from a various chronic liver diseases. Thepathological basis of fibrosis is the disturbance ofproduction and degradation of the extracellularmatrix (ECM), which causes accumulation of ECMin the liver[1,2].Ji Yao Wang Qi Sheng Zhang Ji Sheng Guo Mei Yu Hu Department of Gastroenterology, Zhongshan Hospital, Medical Center, Fu Dan University Shanghai Medical University), Shanghai 200032, China 2001World Journal of Gastroenterology2001,7,1:29
4Analysis of the Results of Two Nationwide Surveys on Clonorchis sinensis Infection in China显示文摘Objective To examine Clonorchis sinensis infection in China and evaluate the effectiveness of efforts to prevent and control it,two nationwide surveys were undertaken in 31 provinces,autonomous regions,and municipalities(PAMs) during 1988-92(the 1990 survey) and during 2001-04(the 2003 survey).Methods During the period 2001-04,two sampling methods were applied.The first method repeated the stratified cluster random sampling used in the 1990 survey;the second method applied two-characteristic stratified cluster random sampling in 27 PAMs-the 2003 endemic area(EA) survey.The Kato-Katz thick smear method was used for the nationwide survey.Results The infection rates of Clonorchis sinensis in the 1990 and 2003 surveys were 0.311% and 0.579%,respectively.The infection rate was 2.40% in the 2003 EA survey,and it was estimated that 12.49 million people in China were infected with Clonorchis sinensis.Conclusion The 2003 survey showed that the standardized infection rate of Clonorchis sinensis increased by 74.85% compared with the 1990 survey.The infection rate in males was higher than in females;the infection rate among people eating raw fish or eating out frequently was higher than among those who did not.CHEN Ying Dan ZHOU Chang Hai XU Long Qi 2012Biomedical and Environmental Sciences2012,25,2:22
5One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang 2017Cell Research2017,27,7:21
6Hippocampal impairments are associated with intermittent hypoxia of obstructive sleep apnea显示文摘妨碍的睡觉呼吸暂停( OSA )是最普通的睡觉相关的呼吸混乱,作为经常的上面的航线倒塌被描绘, obstruction.It 是可对待的混乱但是如果留给未经治疗在几机关 systems.The 健康风险与复杂并发症被联系到 OSA 病人表演有尖锐心血管的事件的一个强壮的协会,并且与长期的 conditions.To 中央神经系统,行为的 OSA 原因和包括白天瞌睡的 neuropsychologic 赤字,消沉,Feng Jing Wu Qi Zhang Dan Chen Bao-yuan 2012Chinese Medical Journal2012,,4:18
7Comparison of Undernutrition Prevalence of Children under 5 Years in China between 2002 and 2013显示文摘Objective To describe the undernutrition status of children under 5-year in China, and study the trend between 2002 and 2013. Methods The study was based on two national surveys. Undernutrition was determined against WHO's 2006 growth standards. The prevalence in 2013 and 2002 was weighted by China sixth National Population Census(2010). The relationship between undernutrition and gender/age groups/different areas use weighted logistic regression. Results The results indicated the overall prevalence of stunting, underweight, and wasting of Chinese children under 5-year was 8.1%, 2.4%, and 1.9% in 2013, respectively. The prevalence of stunting was higher for children aged 12-47 month, while underweight was higher for children aged 48-59 month. The prevalence of undernutrition was higher in rural areas than in urban areas, especially in poor rural areas. There was a decline of stunting, underweight, and wasting between 2002 and 2013 among the children, with greater reduction in rural areas than in urban areas. Conclusion The prevalence of undernutrition of children under 5-year remains high in rural areas especially in poor rural areas in China. It is urgent to take action to control undernutrition in the vulnerable areas and subgroups.YU Dong Mei ZHAO Li Yun YANG Zhen Yu CHANG Su Ying YU Wen Tao FANG Hong Yun WANG Xun YU Dan GUO Qi Ya XU Xiao Li FANG Yue Hui ZHAO Wen Hua YANG Xiao Guang DING Gang Qiang LIANG Xiao Feng 2016Biomedical and Environmental Sciences2016,29,3:18
8Association of Cytokines with Alanine Aminotransferase, Hepatitis B Virus Surface Antigen and Hepatitis B Envelope Antigen Levels in Chronic Hepatitis B显示文摘Ming-Hui Li Yao Lu Lu Zhang Xing-Yue Wang Chong-Ping Ran Hong-Xiao Hao Dan Zhang Xiao-Jing Qu Ge Shen Shu-Ling Wu Wei-Hua Cao Tian-Lin Qi Ru-Yu Liu Lei-Ping Hu Min Chang Wen-Hao Hua Shun-Ai Liu Gang Wan Yao Xie 2018Chinese Medical Journal2018,,15:17
9Phase 1 clinical trial demonstrated that MUC1 positive metastatic seminal vesicle cancer can be effectively eradicated by modified Anti-MUC1 chimeric antigen receptor transduced T cells显示文摘Recent progress in chimeric antigen receptor-modified T-cell(CAR-T cell) technology in cancer therapy is extremely promising, especially in the treatment of patients with B-cell acute lymphoblastic leukemia. In contrast, due to the hostile immunosuppressive microenvironment of a solid tumor, CAR T-cell accessibility and survival continue to pose a considerable challenge, which leads to their limited therapeutic efficacy. In this study, we constructed two anti-MUC1 CAR-T cell lines. One set of CAR-T cells contained SM3 single chain variable fragment(sc Fv) sequence specifically targeting the MUC1 antigen and co-expressing interleukin(IL) 12(named SM3-CAR). The other CAR-T cell line carried the SM3 sc Fv sequence modified to improve its binding to MUC1 antigen(named p SM3-CAR) but did not co-express IL-12. When those two types of CAR-T cells were injected intratumorally into two independent metastatic lesions of the same MUC1+ seminal vesicle cancer patient as part of an interventional treatment strategy, the initial results indicated no side-effects of the MUC1 targeting CAR-T cell approach, and patient serum cytokines responses were positive. Further evaluation showed that p SM3-CAR effectively caused tumor necrosis, providing new options for improved CAR-T therapy in solid tumors.Fengtao You Licui Jiang Bozhen Zhang Qiang Lu Qiao Zhou Xiaoyang Liao Hong Wu Kaiqi Du Youcai Zhu Huimin Meng Zhishu Gong Yunhui Zong Lei Huang Man Lu Jirong Tang Yafen Li Xiaochen Zhai Xiangling Wang Sisi Ye Dan Chen Lei Yuan Lin Qi Lin Yang 2016Science China(Life Sciences)2016,59,4:16
10Plasmacytoid Dendritic Cell Function and Cytokine Network Profiles in Patients with Acute or Chronic Hepatitis B Virus Infection显示文摘Ming-Hui Li Lu Zhang Dan Zhang Wei-Hua Cao Tian-Lin Qi Hong-Xiao Hao Xing-Yue Wang Chong-Ping Ran Xiao-Jing Qu Shun-Ai Liu Yao Lu Ge Shen Shu-Ling Wu Min Chang Ru-Yu Liu Lei-Ping Hu Wen-Hao Hua Gang Wan Jun Cheng Yao Xie 2018Chinese Medical Journal2018,,1:16
11Issues and solutions toward zinc anode in aqueous zinc-ion batteries: A mini review显示文摘Aqueous zinc-ion batteries(ZIBs)have been intensively investigated as potential energy storage devices on account of their low cost,environmental benignity,and intrinsically safe merits.With the exploitation of highperformance cathode materials,electrolyte systems,and in-depth mechanism investigation,the electrochemical performances of ZIBs have been greatly enhanced.However,there are still some challenges that need to be overcome before its commercialization.Among them,the obstinate dendrites,corrosion,and hydrogen evolution reaction(HER)on Zn anodes are critical issues that severely limit the practical applications of ZIBs.To address these issues,various strategies have been proposed,and tremendous progress has been achieved in the past few years.In this article,we analyze the origins and effects of the dendrites,corrosion,and HER on Zn anodes in neutral and mildly acid aqueous solutions at first.And then,a scientific understanding of the fundamental design principles and strategies to suppress these problems are emphasized.Apart from these,this article also puts forward some requirements for the practical applications of Zn anodes as well as several cost-effectivemodifying strategies.Finally,perspectives on the future development of Zn anodes in aqueous solutions are also briefly anticipated.This article provides pertinent insights into the challenges on anodes for the development of highperformance ZIBs,which will greatly contribute to their practical applications.Chunlin Xie Yihu Li Qi Wang Dan Sun Yougen Tang Haiyan Wang 2020Carbon Energy2020,2,4:15
12PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by regulating synaptic transmission in cerebellum显示文摘在充满脯氨酸的 transmembrane 蛋白质的变化(PRRT2 ) 2 与发作性的 kinesigenic 运动障碍(PKD ) 和几另外的发作性的神经病学的疾病被联系,但是 PRRT2 功能和病原的机制仍然保持大部分阴暗。这里,我们证明 PRRT2 是与 SNARE 建筑群和 downregulates 的部件交往的 presynaptic 蛋白质它的形成。Loss-of-function 变异的老鼠显示出显型由小脑的概括发作,过高热,或 optogenetic 刺激触发了的象 PKD 一样。有在小脑的小粒房间(GC ) 的特定的 PRRT2 删除的变异的老鼠概括在 Prrt2 空的老鼠看见的行为的显型。而且,记录在显示出的小脑的片做了在短暂举起的 GC 结果的刺激由 Purkinje 房间开火的抑制跟随了的那 optogenetic。在 PKD 处理使用的 anticonvulsant 药 carbamazepine 也在变异的老鼠减轻了象 PKD 一样行为。一起,我们的调查结果作为 SNARE 建筑群的一个新奇管理者识别 PRRT2 并且提供位于 PRRT2 相关的行为下面的电路机制。Guo-He Tan Yuan-Yuan Liu Lu Wang Kui Li Ze-Qiang Zhang Hong-Fu Li Zhong-Fei Yang Yang Li Dan Li Ming-Yue Wu Chun-Lei Yu Juan-Juan Long Ren-Chao Chen Li-Xi Li Lu-Ping Yin Ji-Wei Liu Xue-Wen Cheng Qi Shen You-Sheng Shu Kenji Sakimura Lu-Jian Liao Zhi-Ying Wu Zhi-Qi Xiong 2018Cell Research2018,28,1:14
13Organochlorine Pesticides(OCPs) in Soils Along the Eastern Slope of the Tibetan Plateau显示文摘Soil samples collected from several sites along an altitudinal transect on the eastern slope of the Tibetan Plateau were analyzed for hexachlorobenzenes(HCHs) and dichlorodiphenyltrichloroethanes(DDTs).The results showed that HCHs and DDTs were found in the soil samples from the remote high altitude areas away from source regions,which confirmed the long-range atmospheric transport phenomenon of these organochlorine pesticides(OCPs) insecticides.The OCP concentrations in the soils had a significant negative correlation with altitude;they showed a trend to increase with decreasing altitude,but the increase was not continuous,being interrupted at some moderate-altitude sites on the transect.The distances from the source region,landforms,soil properties,and physical-chemical properties of OCPs were more important than total organic carbon content to the distribution of OCPs in soils disturbed by human activities.An analysis of the compositions of HCH isomers and DDTs revealed predominantly low ratios of α-HCH to γ-HCH,ranging from 0.06 to 4.79,which suggested current lindane inputs.On the other hand,low p,p-DDT/p,p-DDE and o,p-DDT/p,p-DDT ratios were observed,indicating mainly aged historical DDT residues in the study area.XING Xin-Li QI Shi-Hua ZHANG Yuan YANG Dan J. O. ODHIAMBO 2010Pedosphere2010,20,5:13
14Effectiveness of Acupuncture for Alzheimer's Disease: An Updated Systematic Review and Meta-analysis显示文摘Acupuncture has reportedly improved memory and cognitive impairment in both animal and clinical studies. It may be an effective treatment for Alzheimer's disease (AD). The purpose of this meta-analysis was to review the effectiveness of acupuncture for the treatment of AD. Eight databases were searched for articles published up to and including July 2017, and 13 studies fulfilling the inclusion criteria were identified. The main outcomes assessed were clinical efficacy rate, Mini-Mental State Examination score, Ability of Daily Living Scale score, Alzheimer's Disease Assessment Scale-Cognition score, Hasegawa's Dementia Scale (HDS) score, and adverse events. The methodological quality of the articles was assessed using Cochrane's risk of bias. All the studies compared the efficacy of acupuncture with that of medication, and were published in Chinese journals. Meta-analysis revealed that acupuncture yielded positive results as determined via all the indexes scored except the HDS (95% CI -0.26 to 0.90, Z=0.35, P=0.73). Only one of the studies reported adverse events associated with acupuncture and medication. The rate of adverse events in the medication group was 13%. In most of the studies assessed in the current metaanalysis, acupuncture alone was better than conventional western medicines for the treatment of AD.Qi HUANG Dan LUO Li CHEN Feng-xia LIANG Rui CHEN 2019Current Medical Science2019,39,3:12
15MicroRNA-125b-5p inhibits proliferation and promotes adipogenic differentiation in 3T3-L1 preadipocytes显示文摘以前的证据显示了 microRNA-125b (miR-125b ) 家庭戏在癌症房间生长,开发,区别,和 apoptosis 的规定的重要角色。然而,他们是否贡献 adipocyte 区别的过程,仍然保持不清楚。在现在的学习,我们表明表示 miR-125b-5p 铺平, miR-125b 家庭的一个成员,在进成熟 adipocyte 的 3T3-L1 preadipocyte 的区别期间是戏剧性地起来调整的。进 3T3-L1 房间的 miR-125b-5p 的补充支持了由增加的类脂化合物微滴和 adipocyte 特定的分子的标记的 mRNA 层次证实了的 adipogenic 区别,包括 peroxisome 激活 proliferators 的受体, CCAAT/enhancer-binding 蛋白质,丰满的酸绑定的蛋白质 4,并且脂蛋白脂肪分解酵素,并且由 triglyceride 累积。CCK-8 试金证明 miR-125b-5p 补充显著地禁止了房间增长。流动 cytometry 分析证明 miR-125b-5p 象 mRNA 和 G1/S-related 基因的蛋白质表示一样损害了 G1/S 阶段转变,例如 Cyclin D2, Cyclin D3,和 CDK4。不过,它没在 apoptosis 上有效果。由目标基因预言,另外,我们证明 smad4 可以是在鼠标 3T3-L1 preadipocytes 的 miR-125b-5p 的一个潜在的目标,为一些 miR-125b-5p 的函数的财务。一起拿,这些数据显示 miR-125b-5p 可以在 adipocyte 区别用作一个重要积极管理者,至少部分通过下面调整的 smad4。Dan Ouyang Yaqiong Ye Dongguang Guo Xiaofang Yu Jian Chen Junjie Qi Xiaotong Tan Yuan Zhang Yongjiang Ma Yugu Li 2015Acta Biochimica et Biophysica Sinica2015,47,5:9
16Expression profiling-based clustering of healthy subjects recapitulates classifications defined by clinical observation in Chinese medicine显示文摘Differences between healthy subjects and associated disease risks are of substantial interest in clinical medicine. Based on clinical presentations, Traditional Chinese Medicine(TCM) classifies healthy people into nine constitutions: Balanced, Qi, Yang or Yin deficiency, Phlegm-dampness, Damp-heat, Blood stasis,Qi stagnation, and Inherited special constitutions. In particular, Yang and Yin deficiency constitutions exhibit cold and heat aversion, respectively. However, the intrinsic molecular characteristics of unbalanced phenotypes remain unclear. To determine whether gene expression-based clustering can recapitulate TCM-based classification, peripheral blood mononuclear cells(PBMCs) were collected from Chinese Han individuals with Yang/Yin deficiency(n ? 12 each) and Balanced(n ? 8) constitutions, and global gene expression profiles were determined using the Affymetrix HG-U133 A Plus 2.0 array. Notably,we found that gene expression-based classifications reflected distinct TCM-based subtypes. Consistent with the clinical observation that subjects with Yang deficiency tend toward obesity, series-clustering analysis detected several key lipid metabolic genes(diacylglycerol acyltransferase(DGAT2), acyl-Co A synthetase(ACSL1), and ATP-binding cassette subfamily A member 1(ABCA1)) to be down-and upregulated in Yin and Yang deficiency constitutions, respectively. Our findings suggest that Yin/Yang deficiency and Balanced constitutions are unique entities in their m RNA expression profiles. Moreover,the distinct physical and clinical characteristics of each unbalanced constitution can be explained, in part,by specific gene expression signatures.Ruoxi Yu Dan Liu Yin Yang Yuanyuan Han Lingru Li Luyu Zheng Ji wang Yan Zhang Yingshuai Li Qian-Fei Wang Qi wang 2017Journal of Genetics and Genomics2017,44,4:9
17Non-ablative controlled local hyperthermia for common warts显示文摘GAO Xing-hua GAO Dan SUN Xiu-ping HUO Wei HONG Yu-xiao LI Xiao-dong WANG Xiao-qin QI Rui-qun ZHANG Li GU Xiao-chuan CHEN Hong-duo 2009Chinese Medical Journal2009,,17:9
18Tom70 serves as a molecular switch to determine pathological cardiac hypertrophy显示文摘病理学的心脏的肥大是心失败的不可避免的 forerunner。不管心脏的肥大的病原学, cardiomyocyte mitochondrial 改变总是在这上下文被观察。mitochondrial 先锋蛋白质的进口调节在 pathophysiological 下面维持 mitochondrial 功能的外部膜(汤姆) 建筑群管理的 mitochondrial 的 translocases;然而,它在病理学的心脏的肥大的发展的角色仍然保持不清楚。这里,我们证明 Tom70 是在从人和试验性的动物的病理学的 hypertrophic 心的 downregulated。在 Tom70 表示的减小在 vivo 并且在 vitro 生产了不同病理学的 cardiomyocyte 肥大。指向 Tom70 的眼的 atrophy-1 的有缺点的 mitochondrial 进口触发了细胞内部的氧化应力,它导致了病理学的细胞的回答。重要地,如果有到多样的 pro-hypertrophic 的完整的抵抗的 cardiomyocytes 侮辱,增加了 Tom70 层次。一起,这些结果表明 Tom70 充当安排 hypertrophic 压力和 mitochondrial 回答决定病理学的心脏的肥大的一个分子的开关。Jun Li Man Qi Changming Li Dan Shi Dasheng Zhang Duanyang Xie Tianyou Yuan Jing Feng Yi Liu Dandan Liang Xinran Xu Jinjin Chen Liang Xu Hong Zhang Jiangchuan Ye Fei LV Jian Huang Luying Peng Yi-Han Chen 2014Cell Research2014,24,8:9
19Characterization of polycyclic aromatic hydrocarbons in PM2.s and PMlo in Tanggu District, Tianjin Binhai New Area, China显示文摘Dan YANG Shihua QI Ningombam Linthoingambi DEVI Fang TIAN Ziping HUO Qingyuan ZHU Jing WANG 2012Frontiers of Earth Science2012,6,3:9
20MiR-181a-5p regulates 3T3-L1 cell adipogenesis by targeting Smad7 and Tcf7I2显示文摘Dan Ouyang Lifeng Xu Lihua zhang Dongguang Guo Xiaotong Tan Xiaofang Yu Junjie Qi Yaqiong Ye Qihong Liu Yongjiang Ma Yugu Li 2016Acta Biochimica et Biophysica Sinica2016,48,11:9
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