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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Epigenetic reprogramming, gene expression and in vitro development of porcine SCNT embryos are significantly improved by a histone deacetylase inhibitor--- m-carboxycinnamic acid bishydroxamide (CBHA)显示文摘施主原子核的不够的 epigenetic reprogramming 被相信是哺乳动物的体的房间的低发展效率的最重要的原因之一原子转移(SCNT ) 。以前的研究两个都显示出那在里面 vitro 并且在老鼠 SCNT 的 vivo 开发,胚胎能被处理与各种各样的 histone deacetylase 禁止者( HDACi )显著地增加包括 Trichostatin A , Scriptaid ,和 m-carboxycinnamic 酸 bishydroxamide ( CBHA ),在哪个仅仅 CBHA 的效果还没在另外的种类被测试了。在这份报纸,我们在猪的 SCNT 胚胎的发展检验 CBHA 处理的效果。我们发现了为 CBHA 处理的最佳剂量和时间:在激活以后为 24 h 与 2 mol/L CBHA 孵化 SCNT 胚胎能从 12.7% ~ 26.5% 增加胚囊率。Immunofluorescence 结果证明在 histone 的 acetylation 的水平 3 离氨酸 9 (AcH3K9 ) ,在 histone 的 acetylation 3 离氨酸 18 (AcH3K18 ) ,并且在 histone 的 acetylation 4 离氨酸(AcH4K16 ) 16 在 CBHA 处理以后被提起。同时, CBHA 处理改进了联系象 pou5f1, cdx2,和象 igf2 一样的印的基因那样的基因的开发的表达式。尽管有这些在 vitro 结果和 histone reprogramming 答应,完整的术语发展显著地没在处理以后被增加。在结论, CBHA 改善在里面猪 SCNT 胚胎的 vitro 开发,增加全球 histone acetylation 并且在早阶段改正一些发展地重要的基因的表示。作为在老鼠 SCNT,我们早在猪显示出那原子 epigenetic reprogramming SCNT 胚胎能被 CBHA 处理修改。 | Yuran Song Tang Hai Ying Wang Runfa Guo Wei Li Liu Wang Qi Zhou | 2014 | Protein & Cell2014，5，5： | 9 |
| 3 | Ubiquitylation of p62/sequestosome1 activates its autophagy receptor function and controls selective autophagy upon ubiquitin stress显示文摘在细胞的 ubiquitin (Ub ) 的改变动态平衡,作为 Ub 知道强调,展示并且影响处于多重条件的细胞的回答,然而,内在的机制不完全地被理解。这里,我们报导 autophagy 受体 p62/sequestosome-1 与结合酶, UBE2D2 和 UBE2D3 的 E2 Ub 交往。内长的 p62 在 Ub 动态平衡的 upregulation 期间经历 E2 依赖的 ubiquitylation,一个条件作为 Ub + 应力称为,那对由 bortezomib 的 Ub overexpression,热吃惊或延长 proteasomal 抑制内在,化学疗法的药。p62 的 Ubiquitylation 破坏 p62 的 UBA 领域的 dimerization,解放它的能力认出为选择 autophagy 的 polyubiquitylated 货物。我们进一步证明这机制可能为在 Ub + 压力条件之上的 autophagy 激活是批评的。机制的描述和在察觉到 Ub 应力并且控制选择 autophagy 的 p62 的规章的角色能帮助理解并且调制细胞的回答到许多内长、环境的挑战,潜在地对 autophagy 相关的病为治疗学的策略的发展打开一条新大街。 | Hong Peng Jiao Yang Guangyi Li Qing You Wen Han Tianrang Li Darning Gao Xiaoduo Xie Byung-Hoon Lee Juan Du Jian Hou Tao Zhang Hai Rao Ying Huang Qinrun Li Rong Zeng Lijian Hui Hongyan Wang Qin Xia Xuemin Zhang Yongning He Masaaki Komatsu Ivan Dikic Daniel Finley Ronggui Hu | 2017 | Cell Research2017，27，5： | 9 |
| 4 | Insulin-like growth factor-1 promotes cell cycle progression via upregulation of cyclin D1 expression through the phosphatidylinositol 3-kinase/nuclear factor-κB signaling pathway in FRTL thyroid cells显示文摘 | Meng REN Xia ZHONG Chun-yan MA Ying SUN Qing-bo GUAN Bin CUI Jun GUO Hai WANG Ling GAO Jia-jun ZHAO | 2009 | Acta Pharmacologica Sinica2009，30，1： | 8 |
| 5 | Relationship between Maternal PBMC HBV cccDNA and HBV Serological Markers and its Effect on HBV Intrauterine Transmission显示文摘Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A [‘HBsA g(+), HBeA g(+), and anti‐HBc(+)’] was significantly higher in the PBMC HBV cccD NA positive group than in the control group(χ^2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccD NA(χ^2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeA g, and PBMC HBV cccD NA, the risk of HBV intrauterine transmission was three times higher(OR = 3.69, 95% CI: 1.30‐10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest(OR = 5.89, 95% CI: 2.35‐14.72) when both PBMC HBV cccD NA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccD NA was directly related to HBV intrauterine transmission. Conclusion PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC‐related markers in prenatal testing. | WANG Dan Dan YI Lin Zhu WU Li Na YANG Zhi Qing HAO Hai Yun SHI Xiao Hong WANG Bo FENG Shu Ying FENG Yong Liang WANG Su Ping | 2019 | Biomedical and Environmental Sciences2019，32，5： | 8 |
| 6 | Malnutrition and Quality of Life in Chinese Cancer Patients: a Clinical Study of 23,994 Subjects显示文摘Background Malnutrition is common in patients with cancer,and can negatively impact their quality of life(QoL)and even survival.However,there is currently no large data available on the prevalence of malnutrition in Chinese cancer patients.This study evaluated the prevalence of malnutrition and the QoL of Chinese patients with locoregional,recurrent or metastatic cancer.Methods We conducted a nationwide observational,multi-center,hospital-based cross-sectional study within the Chinese Society of Nutritional Oncology(CSNO)Network.All of the patients were diagnosed with one of the following 18 different types of malignant tumors:lung cancer,gastric cancer,liver cancer,colorectal cancer,breast cancer,esophageal cancer,cervical cancer,endometrial cancer,nasopharyngeal carcinoma,malignant lymphoma,leukemia,pancreatic cancer,ovarian cancer,prostate cancer,bladder cancer,brain cancer,biliary tract malignant tumors or gastrointestinal stromal tumors.These patients were enrolled from 72 hospitals located in different regions of China.The patients’nutritional status was evaluated based on the body mass index(BMI),loss of bodyweight,laboratory measurements and patient generated-subjective global assessment(PG-SGA)scores.The cancer patients’physical status and QoL were assessed by the Karnofsky Performance Status(KPS)questionnaire and the European Organization for Research and Treatment of Cancer(EORTC)QLQ-C30 questionnaire,respectively.Results From December 2013 to April 2016,23,994 patients hospitalized for cancer treatment(such as surgery,chemotherapy or radiotherapy)were enrolled in the study.The patients included 12,494(52.9%)males and 11,124(47.1%)females.The mean age was 55.8±13.7 years.The proportions of patients in cancer stagesⅠ,Ⅱ,Ⅲ,Ⅳand uncertain were 11.5%,20.3%,27.5%,30.2%and 10.5%,respectively.Among the 23,994 inpatients,the proportions of patients who were underweight(BMI<18.5 kg/m2),normal(18.5 kg/m2 | Hong Xia Xu Chun Hua Song Zhen Ming Fu Chang Wang Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Jiu Wei Cui Wei Li Han Ping Shi the Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2021 | Journal of Nutritional Oncology2021，6，1： | 8 |
| 7 | Mechanism of exogenous nucleic acids and their precursors improving the repair of intestinal epithelium after 7-irradiation in mice显示文摘AIM To clone expressed genes associated withrepair of irradiation-damaged mice intestinalgland cells treated by small intestinal RNA,andto explore the molecular mechanism ofexogenous nucleic acids improving repair ofintestinal crypt.METHODS The animal mode of test group andcontrol group was established,forty-five micebeing irradiated by γ ray were treated with smallintestinal RNA as test group,forty mice beingirradiated by γ ray were treated withphysiological saline as control group,five micewithout irradiation were used as normal control,their jejunal specimens were collectedrespectively at 6h,12h,24h,4d and 8d afterirradiation.Then by using LD-PCR based onsubtractive hybridization,these gene fragmentsdifferentially expressed between test group andcontrol group were obtained,and then werecloned into T vectors as well as beingsequenced.Obtained sequences were screenedagainst.GeneBank,if being new sequences,they were submitted to GeneBank.RESULTS Ninety clones were associated withrepair of irradiation-damaged intestinal glandcells treated by intestinal RNA.These clonesfrom test group of 6h,12h,24h,4d and 8dwere respectively 18,22,25,13,12.By screening against GeneBank,18 of which werenew sequences,the others were dramaticallysimilar to the known sequences,mainly similarto hsp,Nmi,Dutt1,alkaline phosphatase,homeobox,anti-CEA ScFv antibody,arginine/serine kinase and BMP-4,repA.Eighteen genefragments were new sequences,their acceptnumbers in GeneBank were respectivelyAF240164-AF240181.CONCLUSION Ninety clones were obtained tobe associated with repair of irradiation-damagedmice intestinal gland cells treated by smallintestinal RNA,which may be related toabnormal expression of genes and matchedproteins of hsp,Nmi,Duttl,Na,K-ATPase,alkalineph-osphatase,glkA,single strandedreplicative centromeric gene as well as 18 newsequences. | Da Xiang Cui~1 Guei Ying Zeng~2 Feng Wang~1 Jun Rong Xu~1 Dong Qing Ren~2 Yan Hai Guo~1 Fu Rong Tian~2 Xiao Jun Yan~1 Yu Hou~1 Cheng Zhi Su~1 1 Institute of Genetic Diagnosis of the Fourth Military Medical University,Xi’an 710032,China 2 Department of Irradiation Medicine of the Fourth Military Medical University,Xi’an 710032,China | 2000 | World Journal of Gastroenterology2000，6，5： | 8 |
| 8 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 9 | Increased Chondrocyte Apoptosis in Kashin-Beck Disease and Rats Induced by T-2 Toxin and Selenium Deficiency显示文摘Objective To investigate chondrocyte apoptosis and the expression of biochemical markers associated with apoptosis in Kashin-Beck disease(KBD) and in an established T-2 toxin-and selenium(Se) deficiency-induced rat model. Methods Cartilages were collected from the hand phalanges of five patients with KBD and five healthy children. Sprague-Dawley rats were administered a selenium-deficient diet for 4 weeks prior to T-2 toxin exposure. The apoptotic chondrocytes were observed by terminal deoxynucleotidyl transferase d UTP nick end labeling staining. Caspase-3, p53, Bcl-2, and Bax proteins in the cartilages were visualized by immunohistochemistry, their protein levels were determined by Western blotting, and m RNA levels were determined by real-time reverse transcription polymerase chain reaction. Results Increased chondrocyte apoptosis was observed in the cartilages of children with KBD. Increased apoptotic and caspase-3-stained cells were observed in the cartilages of rats fed with normal and Se-deficient diets plus T-2 toxin exposure compared to those in rats fed with normal and Se-deficient diets. Caspase-3, p53, and Bax proteins and m RNA levels were higher, whereas Bcl-2 levels were lower in rats fed with normal or Se-deficiency diets supplemented with T-2 toxin than the corresponding levels in rats fed with normal diet. Conclusion T-2 toxin under a selenium-deficient nutritional status induces chondrocyte death, which emphasizes the role of chondrocyte apoptosis in cartilage damage and progression of KBD. | YANG Hao Jie ZHANG Ying WANG Zhi Lun XUE Sen Hai LI Si Yuan ZHOU Xiao Rong ZHANG Meng FANG Qian WANG Wen Jun CHEN Chen DENG Xiang Hua CHEN Jing Hong | 2017 | Biomedical and Environmental Sciences2017，30，5： | 7 |
| 10 | The prevalence of transfusion transmitted virus infection in blood donors显示文摘INIRODUCIIONA newly discovered DNA virus,transfusiontransmitted virus (TTV),was reported as a cause ofpost-transfusion hepatitis of unknown etiology inJapan.In order to investigate TTV prevalence insouthern China,a study was carried out amongblood donors,patients with liver diseases andhemodialysis to determine the epidemiologicalcharateristics. | Cheng Hui Huang Yu Sen Zhou Ru Guang Chen Chun Ying Xie Hai Tao Wang | 2000 | World Journal of Gastroenterology2000，6，2： | 7 |
| 11 | Epidemiological Characteristics and Risk Factors of the Pertussis in Infants < 12 Months of Age in Tianjin, China显示文摘The epidemiologic characteristics of 631 infant pertussis cases<12 months,which accounted for57.42%of the total cases,were analyzed by descriptive epidemiology in Tianjin,between 2010and 2015.The incidence of infants was104.66/100,000,which was 118 times higher than in other age groups(P<0.001).The primary type of pertussis simultaneously presented in households was adult-to-infant(70.51%).The parents were identified as the source of infection in 80.18%of cases for infants.The positive rate of placental antibody transfer was 31.06%and 3.13%for3-month-old infants.Infants presented the highest age-specific pertussis incidence.The most important reason was parents were the important sources of infection,and secondly the lower level of antibodies in neonates and the rapid waning of maternal antibody titer. | HUANG Hai Tao GAO Zhi Gang LIU Yong WANG Li Juan LIU Yan Ping ZHANG Ying | 2017 | Biomedical and Environmental Sciences2017，30，7： | 7 |
| 12 | IL-6 and INF-γ levels in patients with brucellosis in severe epidemic region,Xinjiang,China显示文摘Background:The incidence of brucellosis,which is caused by the Brucella species of bacteria,is rapidly rising worldwide;however,few studies have investigated the immune response to this pathogen and clinical biochemical features.In this paper,we examined the levels of various cytokines and inflammatory factors as well as clinical course characteristics in patients with brucellosis,in order to provide evidence for the diagnosis,assessment,and prognosis of this infectious disease.Methods:A total of 191 brucellosis inpatients(50 acute cases and 141 chronic cases),as well as 60 healthy control subjects,were included in the analysis.We investigated changes in the levels of six cytokines(IL-4,IL-6,IL-10,IL-17,TNF-α,INF-γ)and related clinical biochemical markers in patients with acute and chronic brucellosis in Xinjiang,China.Possible factors were statistically analyzed using the t test,χ2 test,z test and a multivariate logistic stepwise regression test.Results:We found that IL-4,IL-6,IL-10,IL-17,IFN-γ,and TNF-αlevels were higher in those with brucellosis than in controls(P<0.05).With regard to disease progression,procalcitonin(PCT)and C-reactive protein(CRP)levels were significantly higher in those with an acute infection compared to chronic cases(P<0.05).We found that the expression of all six cytokines tested was closely related to the degree of brucellosis using univariate logistic regression;however,only IL-6 and INF-γlevels were independent factors associated with the severity of brucellosis.Conclusions:Assessing cytokine levels in patients with acute and chronic brucellosis is not only useful for detecting the immune response,but can also be indicative of the severity of brucellosis.In particular,we propose IL-6 and INF-γlevels may be useful independent predictive factors in the clinical evaluation and diagnosis of brucellosis. | Zhi-Qiang Lin Guo-Yue Lin Wen-Wen He Chi Zhang Rui Zhang Yuan-Da Li Fan Wang Ying Qin Li Duan Dou-Dou Zhao Xiao-Juan Qu Hui Gao Hai Jiang | 2020 | Infectious Diseases of Poverty2020，9，3： | 7 |
| 13 | Validity of the Chinese Version of the Patient-Generated Subjective Global Assessment (PG-SGA) in Lung Cancer Patients显示文摘The prevalence of malnutrition is high among oncology patients in China. Although the Patient- Generated Subjective Global Assessment (PG-SGA) is widely recognized as an important nutritional assessment tool, it has not been validated for Chinese cancer patients. The purpose of this study was to conduct an analysis of nutrition screening and assessment data to validate the Chinese version of the PG-SGA in Chinese patients with lung cancer. This was an observational, cross-sectional study of 2,000 consecutive adult patients with lung cancer treated at several tertiary hospitals in China. Anthropometric and patient descriptive data were collected. The PG-SGA generated a score for the nutritional risk and a global rating for the nutritional status. The internal consistency, external consistency, and construct validity were evaluated, and a known-groups comparison and principal component analyses were conducted to evaluate the reliability and validity of the PG-SGA. The known-groups comparison demonstrated the ability of the PG-SGA to differentiate lung cancer patients of different nutritional and body mass index (BMI) groups. A confirmatory factor analysis supported the original four-factor structure of the PG-SGA. In terms of the internal and external consistency, the PG-SGA demonstrated intra-class correlation coefficients (ICC) up to 0.987. Alternative form validity was also demonstrated between Box 4 of the PG-SGA and the Karnofsky Performance Status score, with excellent external consistency (r = 0.972, P < 0.001). The construct validity was supported, and selected questionnaire dimensions were evident in the principal component analysis. Significant Spearman correlations (P < 0.001) were demonstrated. The Chinese version of the PG-SGA demonstrated significant reliability and sufficient exploratory properties to support its validity. It seems to be a valid tool that can be used to access the nutritional status of Chinese lung cancer patients. The validity of the PG-SGA in Chinese cancer patients warrants further investigation in other cancer types. | Zhen Ming Fu Hong Xia Xu Chun Hua Song Wei Li Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Chang Wang Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers (INSCOC) Group | 2016 | Journal of Nutritional Oncology2016，1，1： | 7 |
| 14 | Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries显示文摘Objective To study the types of subspecies of Francisella tularensis from China and to investigate the genetic relationships between F.tularensis strains from China and from other countries.Methods Ten strains of F.tularensis isolated from China were amplified by using typing primers C1/C4 and RD1.On the basis of the lengths of the polymerase chain reaction (PCR) products,it was concluded that these strains of F.tularensis belonged to the same subspecies.At the same time,the fopA,tul4,and 16S rRNA genes of the 10 strains were amplified,and a three‐gene based phylogenetic analysis was performed using the Molecular Evolutionary Genetics Analysis software version 4.0.Results The 10 strains of F.tularensis from China were all identified as belonging to subspecies holarctica (type B).We found no direct relationship between the genotypes of F.tularensis subsp.holarctica and the geographical area from where they were isolated.Conclusion The F.tularensis strains isolated from North China mainly belong to subspecies holarctica (type B).The strains of F.tularensis subsp.holarctica from China may have evolved earlier than those from Europe and North America. | WANG YanHua HAI Rong ZHANG ZhiKai XIA LianXu CAI Hong LIANG Ying SHEN XiaoNa YU DongZheng | 2011 | Biomedical and Environmental Sciences2011，24，3： | 7 |
| 15 | Altered Resting-State Signals in Patients with Acute Stroke In or Under the Thalamus显示文摘Previous studies have suggested that cortical functional reorganization is associated with motor recovery after stroke and that normal afferent sensory information is very important in that process.In this study,we selected patients who had a stroke in or under the thalamus,with potentially impaired afferent sensory information and analyzed the differences between these patients and healthy controls at three levels:brain regions,the functional connectivity between brain areas,and the whole-brain functional network.Compared with healthy controls,regional homogeneities in the left middle temporal gyrus decreased and functional connectivity between the left middle temporal gyrus and the stroke area increased in the patients.However,there was no significant change in the wholebrain functional network.By focusing on stroke located in or under the thalamus,our study contributes to wider inquiries into understanding and treating stroke. | Lijun Chen Chuanfu Li Jian Zhai Anqin Wang Qin Song Ying Liu Ru Ma Long Han Yamikani Ndasaukas Xiaoming Li Hai Li Xiaochu Zhang | 2016 | Neuroscience Bulletin2016，32，6： | 6 |
| 16 | DNA cleavage mediated by water-soluble manganese corrole显示文摘水的 DNA 像核酸酶的活动可溶的锰 corrole 5, 10, 15-tris (N-methyl-4-pyridyl )- corrolatomanganese (III )(MnIIITMPyC ) 被调查了。MnIIITMPyC 可以经由由吸收系列,粘性大小和 CD 系列显示了的外面的沟绑定模式强烈绑在 DNA。MnIIITMPyC 面对 hydroperoxide 在 DNA 氧化劈开展出了优秀催化活动。 | Jun Lu Hai Yang Liu Lei Shi Xiang Li Wang Xiao Ying Lei Zhang Liang Nian Ji Lin Quan Zang Chi K. Chang | 2011 | Chinese Chemical Letters2011，22，1： | 6 |
| 17 | Effects of Outdoor Temperature on Blood Pressure in a Prospective Cohort of Northwest China显示文摘Objective The relationship between outdoor temperature and blood pressure(BP) has been inconclusive. We analyzed data from a prospective cohort study in northwestern China to investigate the effect of outdoor temperature on BP and effect modification by season.Methods A total of 32,710 individuals who participated in both the baseline survey and the first follow-up in 2011–2015 were included in the study. A linear mixed-effect model and generalized additive mixed model(GAMM) were applied to estimate the association between outdoor temperature and BP after adjusting for confounding variables.Results The mean differences in systolic blood pressure(SBP) and diastolic blood pressure(DBP)between summer and winter were 3.5 mm Hg and 2.75 mm Hg, respectively. After adjusting for individual characteristics, meteorological factors and air pollutants, a significant increase in SBP and DBP was observed for lag 06 day and lag 04 day, a 0.28 mm Hg(95% CI: 0.27–0.30) per 1 °C decrease in average temperature for SBP and a 0.16 mm Hg(95% CI: 0.15–0.17) per 1 °C decrease in average temperature for DBP, respectively. The effects of the average temperature on both SBP and DBP were stronger in summer than in other seasons. The effects of the average temperature on BP were also greater if individuals were older, male, overweight or obese, a smoker or drinker, or had cardiovascular diseases(CVDs), hypertension, and diabetes.Conclusions This study demonstrated a significant negative association between outdoor temperature and BP in a high-altitude environment of northwest China. Moreover, BP showed a significant seasonal variation. The association between BP and temperature differed by season and individuals' demographic characteristics(age, gender, BMI), unhealthy behaviors(smoking and alcohol consumption), and chronic disease status(CVDs, hypertension, and diabetes). | ZHENG Shan WANG Min Zhen CHENG Zhi Yuan KANG Feng NIE Yong Hong MI Xiu Ying LI Hai Yan JIN Lan ZHANG Ya Wei BAI Ya Na | 2021 | Biomedical and Environmental Sciences2021，34，2： | 5 |
| 18 | Stem cell lineage in body layer specialization and vascular patterning of rice root and leaf显示文摘Since the first appearance of vascular plants during evolution, the plant body has become specialized for adaption to land conditions. Much of our knowledge of plant body specialization and the origins of tissues from stem cells have been obtained from studies on the dicot Arabidopsis thaliana. However, less is known about plant body specialization in monocots, another important branch of angiosperms. In this study, we analyzed stem cell lineage and differentiation during development of the root and leaf of the monocot model plant rice(Oryza sativa). Our results showed that three body layers of rice are established from stem cells accompanied by progressively reduced pluripotency. Layer 1(L1) is a single-cell layer of epidermis; L2 is the cortex/endodermis in the root and the mesophyll in the leaf; and L3 is the site of vascular initiation. At least two common steps in vascular development are shared between rice root and leaf. The preprocambium divides to form the procambium and root pericycle or leaf outer sheath. The procambium further differentiates into the xylem, phloem and circumambient cells. We found that the outer sheath of leaf vascular bundles originates not only from the preprocambium of L3,but also from the mesophyll precursor cells of L2. In addition, WUSCHEL-RELATED HOMEOBOX(WOX)genes are expressed in not only the stem cell niche but also metaxylem precursor in rice. This pattern differs from that of homologs in Arabidopsis, suggesting that WOX functions have been recruited in different stem cells in dicots and monocots. | Minhuan Zeng Bo Hu Jiqin Li Guifang Zhang Ying Ruan Hai Huang Hua Wang Lin Xu | 2016 | Science Bulletin2016，61，11： | 5 |
| 19 | Preparation and characterization of porous TiO_2/ZnO composite nanofibers via electrospinning显示文摘多孔的 TiO2/ZnO 合成 nanofibers 被 electrospinning 技术成功地第一次准备了。它被锻烧 TiO2/ZnCl2/PVP 产生[PVP:poly (乙烯基 pyrrolidone )] nanofibers,是从 TiO2 的一个混合答案的 electrospun, ZnCl2 和 PVP。传播电子显微镜学(TEM ) 和衍射(XRD ) 分析的 X 光检查被用来识别 TiO2/ZnO nanofibers 和无机的 TiO2/ZnO 纤维的形成的形态学。TiO2/ZnO 纤维的多孔的结构被 N2 adsoption/desorption 等温线描绘。表面 photovoltage 光谱学(SPS ) 和揭示的大小预付多孔的 TiO2/ZnO 合成 nanofibers 和结果的性质的 photocatalytic 活动与纯 TiO2 nanofibers,纯 ZnO nanofibers 和 TiO2/ZnO nanoparticles 相比。 | Hai Ying Wang Yang Yang Xiang Li Li Juan Li Ce Wang | 2010 | Chinese Chemical Letters2010，21，9： | 5 |
| 20 | The genome-wide mutational landscape of pituitary adenomas显示文摘 | Zhi-Jian Song Zachary J Reitman Zeng-Yi Ma Jian-Hua Chen Qi-Lin Zhang Xue-Fei Shou Chuan-Xin Huang Yong-Fei Wang Shi-Qi Li Ying Mao Lian-g-Fu Zhou Bao-Feng Lian Hai Yan Yong-Yong Shi Yao Zhao | 2016 | Cell Research2016，26，11： | 5 |
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