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| 1 | Emergence and prevalence of naturally occurring lower virulent African swine fever viruses in domestic pigs in China in 2020显示文摘African swine fever virus(ASFV)has been circulating in China for more than two years,and it is not clear whether the biological properties of the virus have changed.Here,we report on our surveillance of ASFVs in seven provinces of China,from June to December,2020.A total of 22 viruses were isolated and characterized as genotype II ASFVs,with mutations,deletions,insertions,or short-fragment replacement occurring in all isolates compared with Pig/HLJ/2018(HLJ/18),the earliest isolate in China.Eleven isolates had four different types of natural mutations or deletion in the EP402R gene and displayed a nonhemadsorbing(non-HAD)phenotype.Four isolates were tested for virulence in pigs;two were found to be as highly lethal as HLJ/18.However,two non-HAD isolates showed lower virulence but were highly transmissible;infection with 106 TCID50 dose was partially lethal and caused acute or sub-acute disease,whereas 103 TCID50 dose caused non-lethal,sub-acute or chronic disease,and persistent infection.The emergence of lower virulent natural mutants brings greater difficulty to the early diagnosis of ASF and creates new challenges for ASFV control. | Encheng Sun Zhenjiang Zhang Zilong Wang Xijun He Xianfeng Zhang Lulu Wang Wenqing Wang Lianyu Huang Fei Xi Haoyue Huangfu Ghebremedhin Tsegay Hong Huo Jianhong Sun Zhijun Tian Wei Xia Xuewu Yu Fang Li Renqiang Liu Yuntao Guan Dongming Zhao Zhigao Bu | 2021 | Science China(Life Sciences)2021，64，5： | 46 |
| 2 | Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development显示文摘Brain size and cognitive skills are the most dramatically changed traits in humans during evolution and yet the genetic mechanisms underlying these human-specific changes remain elusive.Here,we successfully generated 11 transgenic rhesus monkeys(8 first-generation and 3 second-generation)carrying human copies of MCPH1,an important gene for brain development and brain evolution.Brain-image and tissue-section analyses indicated an altered pattern of neural-cell differentiation,resulting in a delayed neuronal maturation and neural-fiber myelination of the transgenic monkeys,similar to the known evolutionary change of developmental delay(neoteny)in humans.Further brain-transcriptome and tissue-section analyses of major developmental stages showed a marked human-like expression delay of neuron differentiation and synaptic-signaling genes,providing a molecular explanation for the observed brain-developmental delay of the transgenic monkeys.More importantly,the transgenic monkeys exhibited better short-term memory and shorter reaction time compared with the wild-type controls in the delayed-matching-to-sample task.The presented data represent the first attempt to experimentally interrogate the genetic basis of human brain origin using a transgenic monkey model and it values the use of non-human primates in understanding unique human traits. | Lei Shi Xin Luo Jin Jiang Yongchang chen Cirong Liu Ting Hu Min Li Qiang Lin Yanjiao Li Jun Huang Hong Wang Yuyu Niu Yundi Shi Martin Styner Jianhong Wang Yi Lu Xuejin Sun Hualin Yu Weizhi Ji Bing Su | 2019 | National Science Review2019，6，3： | 12 |
| 3 | pH-sensitive polymeric micelles triggered drug release for extracellular and intracellular drug targeting delivery显示文摘Most of the conventional chemotherapeutic agents used for cancer chemotherapy suffer from multidrug resistance of tumor cells and poor antitumor efficacy.Based on physiological differences between the normal tissue and the tumor tissue,one effective approach to improve the efficacy of cancer chemotherapy is to develop pH-sensitive polymeric micellar delivery systems.The copolymers with reversible protonationedeprotonation core units or acid-liable bonds between the therapeutic agents and the micelle-forming copolymers can be used to form pH-sensitive polymeric micelles for extracellular and intracellular drug smart release.These systems can be triggered to release drug in response to the slightly acidic extracellular fluids of tumor tissue after accumulation in tumor tissues via the enhanced permeability and retention effect,or they can be triggered to release drug in endosomes or lysosomes by pH-controlled micelle hydrolysis or dissociation after uptake by cells via the endocytic pathway.The pH-sensitive micelles have been proved the specific tumor cell targeting,enhanced cellular internalization,rapid drug release,and multidrug resistance reversal.The multifunctional polymeric micelles combining extracellular pH-sensitivity with receptor-mediated active targeting strategies are of great interest for enhanced tumor targeting.The micelles with receptor-mediated and intracellular pH targeting functions are internalized via receptor-mediated endocytosis followed by endosomal-pH triggered drug release inside the cells,which reverses multidrug resistance.The pH sensitivity strategy of the polymeric micelles facilitates the specific drug delivery with reduced systemic side effects and improved chemotherapeutical efficacy,and is a novel promising platform for tumor-targeting drug delivery. | Yanhua Liu Wenping Wang Jianhong Yang Chengming Zhou Jin Sun | 2013 | Asian Journal of Pharmaceutical Sciences2013，8，3： | 11 |
| 4 | Soil phosphorus bioavailability assessed by XANES and Hedley sequential fractionation technique in a glacier foreland chronosequence in Gongga Mountain, Southwestern China显示文摘The primary productivity of terrestrial ecosystems is influenced by soil phosphorus bioavailability, which depends largely on chemical fractions of phosphorus. The sequential fractionation technique developed by Hedley et al. or its subsequent modification is a well-known method to determine soil phosphorus forms. Hedley sequential fractionation technique separates the phosphorus into fractions based on their different chemical solubilities in extractants with certain chemical properties. Recently, synchrotron-based X-ray absorption near edge structure(XANES) spectroscopy has been employed to measure soil phosphorus species directly and non-invasively. The XANES method provides information concerning local structure and chemical information of target elements at a molecular level. Thus, it can distinguish phosphorus fractions bound by metal oxides or hydroxides(such as Fe, Al, and Ca). In this present work, the phosphorus speciation of topsoil along a glacial foreland chronosequence in Gongga Mountain is determined using these two methods. The changes in soil phosphorus bioavailability along the 120-year-old chronosequence are assessed based on comparisons of the results obtained by these two methods. The results indicate that Hedley sequential fractionation technique shows a greater ability to determine soil bioavailable phosphorus(Resin-P and NaCHO3-P), while XANES is effective in distinguishing phosphorus bound by metal compounds. In the chronosequence, Ca- and Al-bound phosphorus were derived mainly from primary minerals, whose phosphorus contents decreased within 120 years of moraine weathering and soil development. The content of soil bioavailable phosphorus increased rapidly after 30 years since deglaciation. The increasing phosphorus bioavailability promoted the colonizing and primary succession vegetation. | WU YanHong Jrg PRIETZEL ZHOU Jun BING HaiJian LUO Ji YU Dong SUN ShouQin LIANG JianHong SUN HongYang | 2014 | Science China Earth Sciences2014，57，8： | 6 |
| 5 | Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain显示文摘The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK12 transfected IBRS-2 cells and the re- covered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect im- munofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we ob- tained and the virus derived from pSVOK12 had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK12, will be very useful in studies of the anti- genicity, virulence, pathogenesis, maturation and replication of SVDV. | ZHENG Haixue LIU Xiangtao SHANG Youiun WU Jinyan BAI Xingwen JIN Ye SUN Shiqi GUO Huichen TIAN Hong FENG Xia YIN Shuanghui GUO Jianhong CONG Guozheng LIU Zaixin CHANG Huiyun MA Junwu XIE Qingge | 2006 | Chinese Science Bulletin2006，51，17： | 6 |
| 6 | Characterization of a new mutant allele of the Arabidopsis Flowering Locus D (FLD) gene that controls theflowering time by repressing FLC显示文摘Flowering in higher plants is controlled by both the internal and environmental cues. In Arabidopsis, several major genetic loci have been defined as the key switches to control flowering. The Flowering Locus C (FLC) gene has been shown in the autonomous pathway to inhibit the vege-tative-to-reproductive transition. FLC appears to be re-pressed by Flowering Locus D (FLD), which encodes a com-ponent of the histone deacetylase complex. Here we report the identification and characterization of a new mutant allele fld-5. Genetic analysis indicates that fld-5 (in the Was-silewskija background) is allelic to the previously character-ized fld-3 and fld-4 (in the Colombia-0 background). Genetic and molecular analyses reveal that fld-5 carries a frame-shift mutation, resulting in a premature termination of the FLD open reading frame. The FLC expression is remarkably in-creased in fld-5, which presumably attributes to the ex-tremely delayed flowering phenotype of the mutant. | CHEN Ruiqiang ZHANG Suzhi SUN Shulan CHANG Jianhong ZUO Jianru | 2005 | Chinese Science Bulletin2005，50，23： | 6 |
| 7 | Clinicopathological analysis of metaplastic meningioma: report of 15 cases in Huashan Hospital显示文摘Objective: Metaplastic meningioma is a rare subtype of benign meningiomas, classified as WHO grade I with well prognosis. Here we presented our experiences on 15 cases of metaplastic meningioma, to investigate the clinicopathological features, therapies and prognosis of these cases. Methods: 15 patients underwent surgical treatment for intracranial metaplastic meningioma between 2001 and 2010 at Neurosurgery Department of Huashan Hospital, Shanghai, China. The clinical data, radiological manifestation, treatment strategy, pathological findings and prognosis of all patients were analyzed retrospectively. Results: Among the 15 cases (10 males and 5 females), the age ranged from 22 to 74 years old (the mean age was 50.67-year old). The clinical manifestations include headache, dizziness, seizure attack, vision decrease, and weakness of bilateral lower limbs. All the patients received surgical treatment, combined with radiotherapy in some cases. In the follow-up period, recurrence occurred in 2 cases, of which 1 patient died of other system complications. Conclusions: Metaplastic meningiomas are characterized by focal or widespread mesenchymal differentiation with formation of bone, cartilage, fat, and xanthomatous tissue elements. Surgical removal is the optimal therapy, and the overall prognosis is well. But recurrence may occur in some cases, thus radiotherapy is necessary for such kind of patients. | Hailiang Tang Huaping Sun Hong Chen Ye Gong Ying Mao Qing Xie Liqian Xie Mingzhe Zheng Daijun Wang Hongda Zhu Xiaoming Che Ping Zhong Kang Zheng Shiqi Li Weimin Bao Jianhong Zhu Xuanchun Wang Xiaoyuan Feng Xiancheng Chen Liangfu Zhou | 2013 | Chinese Journal of Cancer Research2013，25，1： | 6 |
| 8 | Beam Measurement System in NSRL显示文摘This paper describes the beam measurement system for the accelerator of the National Synchrotron Radiation Laboratory (NSRL). The design considerations of the beam measurement instrumentation system for the NSRL are aided by the commissioning of the machine and provide sufficient machine parameters for machine study as well as the common operation. The beam measurement system includes the part for the linac and transport line and the part for the storage ring. The measurement results are presented in the paper. | Sun, Baogen Lu, Ping Wang, Junhua Fang, Zhigao Liu, Jianhong | 2000 | Journal of Systems Engineering and Electronics2000，11，3： | 4 |
| 9 | Experimental and numerical study on the flapping motion of submerged turbulent plane jet显示文摘A submerged,vertical turbulent plane water jet impinging onto a free surface will be self-excited into a flapping oscillation when the jet velocity,leaving the jet orifice,exceeds a critical value.The flapping phenomenon was verified simultaneously in this paper by laser Doppler velocimeter measurement and numerical analyses with volume of fluid approach coupled with a large eddy simulation turbulent model.The general agreement of mean velocities between numerical predictions and experimental results in self-similar region is good for two cases:Reynolds numbers 2090 and 2970,which correspond to the stable impinging jet and flapping jet.Results show that the flapping jet is a new flow pattern for submerged turbulent plane jets with characteristic flapping frequency,and that the decay of the mean velocity along the jet centerline is considerably faster than that of the stable impinging state. | SUN JianHong ZHAO LiQing HSU ChinTsau | 2013 | Science China(Technological Sciences)2013，56，10： | 4 |
| 10 | Flow Characteristics of Double-Cruciform Parachute at Inflating and Inflated Conditions显示文摘The fluid-structure interaction (FSI) between the canopy and flow field on the inflating and inflated conditions is investigated based on the arbitrary Lagrange-Euler (ALE) method,in both a single-and double-cruciform parachute systems.The projection area of canopy is calculated in the inflation process.The flow field characteristics and the interaction between canopies are analyzed.Results showed that,with free stream velocity of 50m/s,overinflation phenomenon would not occur during the inflation process of the double-cruciform-parachute system,because the collision and extrusion of the two canopies during inflation obstructed the oscillation of the inner gores.Concurrently,compared with the single-cruciform parachute,the vortex motion in the wake of double-cruciform-parachute is more intense.Thus the double-cruciform parachute system oscillated at a velocity of 50 m/s with an angle of less than 6.8°.By comparison,the oscillation angle of the single-cruciform parachute was within 3.5° at the velocity of 50m/s.The results are consistent with those of the wind tunnel test. | Fang Ming Sun Jianhong Zhang Tong Hou Bin Zhang Yantai | 2018 | Transactions of Nanjing University of Aeronautics and Astronautics2018，35，6： | 3 |
| 11 | Preventing abnormal NF-κB activation and autoimmunity by Otub1-mediated p100 stabilization显示文摘NF-κB, a family of transcription factors regulating diverse biological processes including immune responses, is activated by canonical and noncanonical pathways based on degradation of IκBα and processing of the IκB-like protein p100, respectively. Although p100 responds to noncanonical NF-κB stimuli for processing, it does not undergo degradation, but rather becomes accumulated, along with canonical NF-κB activation. We show here that the stability of p100 is tightly controlled by a deubiquitinase, Otub1. Otub1 deficiency not only promotes signal-induced p100 processing and noncanonical NF-κB activation but also causes steady-state p100 degradation, leading to aberrant NF-κB activation in the canonical pathway. B-cell-conditional deletion of Otub1 results in B-cell hyperplasia, antibody hyper-production, and lupus-like autoimmunity. Otub1-deficient B cells display aberrantly activated phenotypes and overproduce the cytokine IL-6, contributing to autoimmunity induction. Thus, maintenance of p100 stability by Otub1 serves as an unusual mechanism of NF-κB regulation that prevents autoimmunity. | Yanchuan Li Jin-Young Yang Xiaoping Xie Zuliang Jie Lingyun Zhang Jianhong Shi Daniel Lin Meidi Gu Xiaofei Zhou Haiyan S. Li Stephanie S. Watowich Antrix Jain Sung Yun Jung Jun Qin Xuhong Cheng Shao-Cong Sun | 2019 | Cell Research2019，29，6： | 3 |
| 12 | Intraoperative ultrasound assistance in resection of intracranial meningiomas显示文摘Objective: Intracranial meningiomas, especially those located at anterior and middle skull base, are difficult to be completely resected due to their complicated anatomy structures and adjacent vessels. It's essential to locate the tumor and its vessels precisely during operation to reduce the risk of neurological deficits. The purpose of this study was to evaluate intraoperative ultrasonography in displaying intracranial meningioma and its surrounding arteries, and evaluate its potential to improve surgical precision and minimize surgical trauma. Methods: Between December 2011 and January 2013, 20 patients with anterior and middle skull base meningioma underwent surgery with the assistance of intraoperative ultrasonography in the Neurosurgery Department of Shanghai Huashan Hospital. There were 7 male and 13 female patients, aged from 31 to 66 years old. Their sonographic features were analyzed and the advantages of intraoperative ultrasonography were discussed. Results: The border of the meningioma and its adjacent vessels could be exhibited on intraoperative ultrasonography. The sonographic visualization allowed the neurosurgeon to choose an appropriate approach before the operation. In addition, intraoperative ultrasonography could inform neurosurgeons about the location of the tumor, its relation to the surrounding arteries during the operation, thus these essential arteries could be protected carefully. Conclusions: Intraoperative ultrasonography is a useful intraoperative technique. When appropriately applied to assist surgical procedures for intracranial meningioma, it could offer very important intraoperative information (such as the tumor supplying vessels) that helps to improve surgical resection and therefore might reduce the postoperative morbidity. | Hailiang Tang Huaping Sun Liqian Xie Qisheng Tang Ye Gong Ying Mao Qing Xie Mingzhe Zheng Daijun Wang Hongda Zhu Jianhong Zhu Xiaoyuan Feng Zhenwei Yao Xiancheng Chen Liangfu Zhou | 2013 | Chinese Journal of Cancer Research2013，25，3： | 3 |
| 13 | Large-eddy simulation of the compressible flow past a tabbed cylinder显示文摘The compressible flow past a tabbed cylinder has been studied numerically using large-eddy simulation for a free-stream Mach number M∞=0.75 and a Reynolds number based on the diameter Re=2×105. Because of the passive control of the flow past a tabbed cylinder, the mean drag coefficient of tabbed cylinder is less than that of a corresponding circular cylinder with a drag reduction up to 33%. The fluctuating lift coefficient is greatly suppressed to be nearly zero. Drag reduction due to the shearing process prevails over that due to the compressing process in this flow. Through investigating the mechanisms relevant to passive control of the tab, it is found that suppression of the shear layer instability can lead to a higher-base-pressure distribution, which can reasonably be associated with drag reduction and suppression of the lift fluctuations. The analysis of convective Mach number and self-sustained oscillations phenomenon inside the shear layers indicates that both the compressible effect and high-frequency forcing result in suppression of the shear layer instability of tabbed cylinder. | XU ChangYue ZHAO LiQing SUN JianHong | 2012 | Chinese Science Bulletin2012，57，24： | 3 |
| 14 | Correction of the probabilistic density function of discontinuities spacing considering the statistical error based on negative exponential distribution显示文摘 | Jianhong Ye Yan Zhang Jinzhong Sun Faquan Wu | 2012 | Journal of Structural Geology2012，，： | 2 |
| 15 | Recovery of a Far-Eastern Strain of Tick-Borne Encephalitis Virus with a Full-Length Infectious cDNA Clone显示文摘Tick-borne encephalitis virus(TBEV) is a pathogenic virus known to cause central nervous system(CNS) diseases in humans, and has become an increasing public health threat nowadays. The rates of TBEV infection in the endemic countries are increasing. However, there is no effective antiviral against the disease. This underscores the urgent need for tools to study the emergence and pathogenesis of TBEV and to accelerate the development of vaccines and antivirals. In this study, we reported an infectious c DNA clone of TBEV that was isolated in China(the WH2012 strain). A beta-globin intron was inserted in the coding region of nonstructural protein 1(NS1) gene to improve the stability of viral genome in bacteria. In mammalian cells, the inserted intron was excised and spliced precisely, which did not lead to the generation of inserted mutants. High titers of infectious progeny viruses were generated after the transfection of the infectious clone. The cDNA-derived TBEV replicated efficiently, and caused typical cytopathic effect(CPE) and plaques in BHK-21 cells. In addition, the CPE and growth curve of cDNA-derived virus were similar to that of its parental isolate in cells. Together, we have constructed the first infectious TBEV cDNA clone in China, and the clone can be used to investigate the genetic determinants of TBEV virulence and disease pathogenesis, and to develop countermeasures against the virus. | Penghui Li Chen Yao Ting Wang Tong Wu Wenfu Yi Yue Zheng Yuanjiu Miao Jianhong Sun Zhongyuan Tan Yan Liu Xiaowei Zhang Hanzhong Wang Zhenhua Zheng | 2021 | Virologica Sinica2021，36，6： | 2 |
| 16 | FREQUENCY LOCK—IN PHENOMENON OF FLAPPING MOTION WITH SURFACE WATER WAVE显示文摘To study the effect of a progressive surface wave on the flapping motion of a vertical turbulent plane jet in shallow water,the laser visualizations and measurements were carried out in a water tank.The images of visualization by laser-induced fluorescence(LIF)technique show that the jet flapping motion occurs in the wave environ-ment.Using the wave height gauge and laser Doppler velocimetry(LDV) ,experimental results show that the jet flapping motions indeed lock-in to the wave oscillations if the wave frequency is close to but lower than the nature frequency of jet flapping motion.The phenomenon does not occur when the wave frequency is above the nature frequency of jet flapping motion. | Sun Jianhong Kuang Jun Hsu Chintsau | 2002 | Transactions of Nanjing University of Aeronautics and Astronautics2002，19，1： | 2 |
| 17 | Numerical investigation of bumps and oscillation in the cylindrical membrane inflation显示文摘 | WANG Conglei SUN Jianhong XU Changyue | 2014 | Applied Mechanics and Materials2014，，： | 1 |
| 18 | Reprogramming donor cells with oocyte extracts improves in vitro development of nuclear transfer embryos显示文摘 | Shuang Tang Yongsheng Wang Dong Zhang Yajun Gao Yefei Ma Baoying Yin Jianhong Sun Jun Liu Yong Zhang | 2008 | Animal Reproduction Science2008，，1： | 1 |
| 19 | Effect of Co content on the structure and electrochemical properties of La 1.5 Mg 0.5 Ni 7? x Co x ( x =0, 1.2, 1.8) hydrogen storage alloys显示文摘 | Faliang Zhang Yongchun Luo Kai Sun Dahui Wang Ruxu Yan Long Kang Jianhong Chen | 2006 | Journal of Alloys and Compounds2006，，1： | 1 |
| 20 | Estimation of the tensile elastic modulus using Brazilian disc by applying diametrically opposed concentrated loads显示文摘 | YE Jianhong WU F Q SUN J Z | 2009 | International Journal of Rock Mechanics & Mining Sciences2009，46，3： | 1 |
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