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| 1 | Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies显示文摘Coronavirus disease 2019(COVID-19),caused by the novel human coronavirus SARS-CoV-2,is currently a major threat to public health worldwide.The viral spike protein binds the host receptor angiotensin-converting enzyme 2(ACE2)via the receptor-binding domain(RBD),and thus is believed to be a major target to block viral entry.Both SARS-CoV-2 and SARS-CoV share this mechanism.Here we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing antibodies.The in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice;however,the cross-neutralizing activity was much weaker,indicating that there are distinct antigenic features in the RBDs of the two viruses.This finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or SARS-CoV-2.It is worth noting that a newly developed SARS-CoV-2 human antibody,HA001,was able to neutralize SARS-CoV-2,but failed to recognize SARS-CoV.Moreover,the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD,representing new binding sites for neutralizing antibodies.Overall,our study has revealed the presence of different key epitopes between SARS-CoV and SARSCoV-2,which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable. | Chunyan Yi Xiaoyu Sun Jing Ye Longfei Ding Meiqin Liu Zhuo Yang Xiao Lu Yaguang Zhang Liyang Ma Wangpeng Gu Aidong Qu Jianqing Xu Zhengli Shi Zhiyang Ling Bing Sun | 2020 | Cellular & Molecular Immunology2020，17，6： | 13 |
| 2 | Radial propagation of magnetospheric substorm-injected energetic electrons observed using a BD-IES instrument and Van Allen Probes显示文摘In cases where substorm injections can be observed simultaneously by multiple spacecraft,they can help elucidate the potential mechanisms of particle transport and energization,of great importance to understanding and modeling the magnetosphere.In this paper,using data returned from the BeiDa-IES(BD-IES) instrument onboard a satellite in an inclined(55°) geosynchronous orbit(IGSO),in combination with two geo-transfer orbiting(GTO) satellite Van Allen Probes(A and B),we analyze a substorm injection event that occurred on the 16 th of October 2015.During this substorm injection,the IGSO onboard BD-IES was outbound,while both Van Allen Probe satellites(A and B) were inbound,a configuration of multiple trajectories that provides a unique opportunity to simultaneously investigate both the inward and outward radial propagation of substorm injection.Indicated by AE/AL indices,this substorm was closely related to an IMF/solar wind discontinuity that showed a sharp change in IMF Bz direction to the north.The innermost signature of this substorm injection was detected by Van Allen Probes A and B at L-3.7,while the outermost signature was observed by the onboard BD-IES instrument at L~\0.These data indicate that the substorm had a global,rather than just local,effect.Finally,we suggest that electric fields carried by fast-mode compressional waves around the substorm injection are the most likely candidate mechanism for the electron injection signatures observed in the inner- and outermost inner magnetosphere. | ZONG QiuGang HAO YiXin ZOU Hong FU SuiYan ZHOU XuZhi REN Jie WANG LingHua YUAN ChongJing LIU ZhiYang JIA XiangHong QUAN Lin LIU Ying WANG YongFu | 2016 | Science China Earth Sciences2016，59，7： | 8 |
| 3 | Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles显示文摘The extraction of nucleic acid is recognized as one of the most essential steps in molecular biology for initiating other downstream applications such as sequencing, amplification, hybridization, and cloning. Many commercial kits and methods are currently available that allow the extraction of only one type of nucleic acids-DNA or RNA. However, in parallel clinical detection of several diseases, a method for simultaneous extraction of both DNA and RNA from the same source is needed in such cases. In this study, a method for simultaneous extraction of DNA and RNA from bacteria based on magnetic nanoparticles(MNPs) was described. Lysis buffers were prepared to help the nucleic acid released and adsorbed to MNPs. Then, two washing buffers were used to remove the contamination of proteins and carbohydrates. The nucleic acids were finally eluted by Deoxyribonuclease(DNase) and Ribonucleases(RNase) free water. Different factors which might affect the purification of the nucleic acid were investigated, and the quantity and quality parameters of the nucleic acid were also recorded. The DNA and RNA extracted from bacteria were then respectively subjected to polymerase chain reaction(PCR) and reverse transcription PCR(RT-PCR) to further confirm its quality. The results indicated that our method can be successfully used to simultaneously extract DNA and RNA from bacteria. | Jiuhai Wang Zeeshan Ali Nianyue Wang Wenbiao Liang Hongna Liu Fu Li Haowen Yang Lei He Libo Nie Nongyue He Zhiyang Li | 2015 | Science China Chemistry2015，58，11： | 5 |
| 4 | Inheritance,Innovation and Development of Wuling Mountain Area in Hubei Province Based on Intellectual Property Rights显示文摘Using the methods of field survey and literature collection,this paper firstly introduces the intellectual property resources of the Wuling Mountain Area in Hubei Province from folk literature and arts,traditional knowledge,biological genetic resources,new plant varieties,patents,geographical indications,and trademarks.Then,it analyzes the main problems in the protection and inheritance,innovation and development of intellectual property rights(IPRs).In addition,it discusses the strategy of inheritance,innovation and high-quality development of this ethnic area in the context of the strategy of strengthening the country with intellectual property rights and the strategy of rural revitalization.Finally,it comes up with recommendations including carrying forward and inheriting folk literature and art,protecting traditional knowledge of traditional craftsmanship,inheriting and innovating to develop traditional Chinese medicine and ethnic medicine,improving the protection and use of geographical indications,and strengthening the IPR protection. | Miaomiao XU Yuanpeng SUN Zhiyang LIU Zhiguo SUN | 2022 | Asian Agricultural Research2022，14，8： | 5 |
| 5 | Intrusion Detection for Wireless Mesh Networks using Finite State Machine显示文摘 | Yi Ping Wu Yue Liu Ning Wang Zhiyang | 2010 | China Communications2010，7，5： | 5 |
| 6 | Current signal amplification strategies in aptamer-based electrochemical biosensor:A review显示文摘Due to their high specificity and affinity towards various targets,along with other unique advantages such as stability and low cost,aptamers are widely applied in analytical techniques.A typical aptamerbased electrochemical biosensor is composed of a aptamer as the biological recognition element and transducer converting the biologic interaction into electrical signals for the quantitative measurement of targets.Improvement of the sensitivity of a biosensor is significantly important in order to achieve the detection of biomolecules with low abundance,and different amplification strategies have been explored.The strategies either employ nanomaterials such as gold nanoparticles to con struct electrodes which can transfer the biological reactions more efficiently,or attempt to obtain enhanced signal through multi-labeled carriers or utilize enzyme mimics to catalyze redox cycling.This review discusses recent advances in signal amplification methods and their applications.Critical assessment of each method is also considered. | Lei He Rongrong Huang Pengfeng Xiao Yuan Liu Lian Jin Hongna Liu Song Li Yan Deng Zhu Chen Zhiyang Li Nongyue He | 2021 | Chinese Chemical Letters2021，32，5： | 3 |
| 7 | C.elegans-based screen identifies lysosome-damaging alkaloids that induce STAT3-dependent lysosomal cell death显示文摘Lysosomes are degradation and signaling centers within the cell,and their dysfunction impairs a wide variety of cellular processes.To understand the cellular effect of lysosome damage,we screened natural smallmolecule compounds that induce iysosomal abnormality using Caenorhabditis elegans (C.elegans)as a model system.A group of vobasinyl-ibogan type bisindole alkaloids (ervachinines A-D)were identified that caused lysosome enlargement in C.elegans macrophage-like cells.Intriguingly,these compounds triggered cell death in the germ line independently of the canonical apoptosis pathway.In mammalian cells, ervachinines A-D induced lysosomal enlargement and damage,leading to leakage of cathepsin proteases, inhibition of autophagosome degradation and necrotic cell death.Further analysis revealed that this ervachinine-induced lysosome damage and lysosomal cell death depended on STAT3 signaling,but not RIP1 or RIP3 signaling.These findings suggest that lysosomedamaging compounds are promising reagents for dissecting signaling mechanisms underlying lysosome homeostasis and lysosome-related human disorders. | Yang Li Yu Zhang Qiwen Gan Meng Xu Xiao Ding Guihua Tang Jingjing Liang Kai Liu Xuezhao Liu Xin Wang Lingli Guo Zhiyang Gao Xiaojiang Hao Chonglin Yang | 2018 | Protein & Cell2018，9，12： | 2 |
| 8 | Achieving high conversion of syngas to aromatics显示文摘Realizing high CO conversion and high aromatics selectivity simultaneously in syngas-to-aromatics(STA)reaction is still challenging.Herein,we report a 57.5%CO conversion along with 74%aromatics selectivity over a composite catalyst consisting of Fe/ZnCr_(2)O_(4)(Fe modified ZnCr_(2)O_(4)spinel)oxide and H-ZSM-5 zeolite.Impregnation of only 3 wt%of Fe onto ZnCr_(2)O_(4)can remarkably increase CO conversion without sacrificing the aromatics selectivity.Oxygen vacancy concentration is improved after impregnating Fe.The highly dispersed iron carbide species is formed during the reaction over Fe/ZnCr_(2)O_(4)spinel oxide.The synergistic effect of oxygen vacancy and iron carbide results in a rapid formation of abundant oxygenated intermediate species,which can be continuously transformed to aromatics in H-ZSM-5.This study provides a new insight into the design of highly efficient catalyst for syngas conversion. | Yi Fu Youming Ni Zhiyang Chen Wenliang Zhu Zhongmin Liu | 2022 | Journal of Energy Chemistry2022，31，3： | 2 |
| 9 | Protective humoral immunity in SARS-CoV-2 infected pediatric patients显示文摘After the rapid spread of SARS-CoV-2 in Wuhan,China,at the beginning of 2020,about 1.5 million confirmed cases and over 80,000 deaths have been reported around 200 countries and territories all over the world and the number continues to increase.However,we still have limited knowledge of this new coronavirus,especially the interaction between SARS-CoV-2 and our immune system. | Yaguang Zhang Jin Xu Ran Jia Chunyan Yi Wangpeng Gu Pengcheng Liu Xinran Dong Hao Zhou Bo Shang Shipeng Cheng Xiaoyu Sun Jing Ye Xuezhen Li Jia Zhang Zhiyang Ling Liyan Ma Bingbing Wu Mei Zeng Wenhao Zhou Bing Sun | 2020 | Cellular & Molecular Immunology2020，17，7： | 2 |
| 10 | Catalytic activity of Cu/ZnO catalysts mediated by MgO promoter in hydrogenation of methyl acetate to ethanol显示文摘Hydrogenation of methyl acetate is a key step in ethanol synthesis from dimethyl ether carbonylation and Cu-based catalysts are widely studied.We report here that the hydrogenation activity of Cu/ZnO catalysts can be enhanced by the addition of MgO promoter.The evolution of crystal phases during coprecipitation and the physicochemical properties of calcined and reduced catalysts by X-ray diffraction(XRD),thermogravimetric(TG)-mass spectrometry(MS),Brunauer-Emmett-Teller(BET),transmission electron microscopy(TEM),N_(2)O titration,in situ CO-Fourier transform infrared spectroscopy(FTIR)and H_(2)-temperature programmed reduction(H_(2)-TPR)reveal that the promoter effect likely lies in the presence of Mg^(2+).A proper amount of Mg^(2+)mediates the precipitation process of Cu and Zn,leading to preferable formation of aurichalcite(Cu_(x)Zn_(1-x))5(CO_(3))_(2)(OH)_(6) crystal phase and a small amount of basic carbonates such as hydrozincite Zn_(5)(CO_(3))_(2)(OH)_(6) and malachite Cu_(2) CO_(3)(OH)_(2).The presence of aurichalcite strengthens the interaction between Cu and Zn species,and thus enhances the dispersity of CuO species and helps generation of Cu^(+)species on reduced catalysts.Furthermore,the performance of Cu/ZnO catalysts exhibits an optimal dependence on the Mg loading,i.e.,17.5%.However,too much Mg^(2+)in the precipitation liquid prohibits formation of aurichalcite but enhances formation of basic nitrates,leading to a dramatically reduced hydrogenation activity.These findings may find applications for optimization of other Cu-based catalysts in a wider range of hydrogenation reactions. | Fang Zhang Zhiyang Chen Xudong Fang Hongchao Liu Yong Liu Wenliang Zhu | 2021 | Journal of Energy Chemistry2021，30，10： | 2 |
| 11 | Bioinformatic analysis of chromatin organization and biased expression of duplicated genes between two poplars with a common whole-genome duplication显示文摘The nonrandom three-dimensional organization of chromatin plays an important role in the regulation of gene expression.However,it remains unclear whether this organization is conserved and whether it is involved in regulating gene expression during speciation after whole-genome duplication(WGD)in plants.In this study,high-resolution interaction maps were generated using high-throughput chromatin conformation capture(Hi-C)techniques for two poplar species,Populus euphratica and Populus alba var.pyramidalis,which diverged~14 Mya after a common WGD.We examined the similarities and differences in the hierarchical chromatin organization between the two species,including A/B compartment regions and topologically associating domains(TADs),as well as in their DNA methylation and gene expression patterns.We found that chromatin status was strongly associated with epigenetic modifications and gene transcriptional activity,yet the conservation of hierarchical chromatin organization across the two species was low.The divergence of gene expression between WGD-derived paralogs was associated with the strength of chromatin interactions,and colocalized paralogs exhibited strong similarities in epigenetic modifications and expression levels.Thus,the spatial localization of duplicated genes is highly correlated with biased expression during the diploidization process.This study provides new insights into the evolution of chromatin organization and transcriptional regulation during the speciation process of poplars after WGD. | Le Zhang Jingtian Zhao Hao Bi Xiangyu Yang Zhiyang Zhang Yutao Su Zhenghao Li Lei Zhang Brian J.Sanderson Jianquan Liu Tao Ma | 2021 | Horticulture Research2021，8，1： | 2 |
| 12 | Direct ink writing of programmable functional silicone-based composites for 4D printing applications显示文摘Polydimethylsiloxane(PDMS)has been widely used in flexible electronics,soft robotics,and bioelectronics.However,the fabrication of PDMS-based devices has mostly relied on conventional approaches,such as casting and molding,thereby limiting their potential.Here we fabricate PDMS-based composites with programmable microstructures by direct ink writing and realize their practical functionalities of four-dimensional(4D)printing.The mechanical,thermomechanical and magnetic properties of the three-dimensional-printed composites can be well tailored by using carbon,metal,or ceramic functional fillers.By taking advantage of the printable,flexible,and magnetic PDMS composites,we demonstrate new practical functionalities of 4D printing by designing programmable architectures,including magnetic-field-driven battery cases and patchworks,as well as arbitrary morphing ceramic structures.In particular,4D-printed batteries are constructed by PDMS-based battery cases for the first time,which can be actuated via external magnetic field.This study broadens the paradigm of 4D printing for prospective applications,such as implant batteries,biomimetic engineering,and customized biomedical devices. | Zhiyang Lyu J.ustin Koh Gwendolyn J.H.Lim Danwei Zhang Ting Xiong Lei Zhang Siqi Liu Junfei Duan Jun Ding John Wang Jinlan Wang Yunfei Chen Chaobin He | 2022 | Interdisciplinary Materials2022，1，4： | 2 |
| 13 | Biomarkers of aging显示文摘Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological status.Although a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly characterized.An immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this need.Here,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory factors.To fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant. | Aging Biomarker Consortium Hainan Bao Jiani Cao Mengting Chen Min Chen Wei Chen Xiao Chen Yanhao Chen Yu Chen Yutian Chen Zhiyang Chen Jagadish K Chhetri Yingjie Ding Junlin Feng Jun Guo Mengmeng Guo Chuting He Yujuan Jia Haiping Jiang Ying Jing Dingfeng Li Jiaming Li Jingyi Li Qinhao Liang Rui Liang Feng Liu Xiaoqian Liu Zuojun Liu Oscar Junhong Luo Jianwei Lv Jingyi Ma Kehang Mao Jiawei Nie Xinhua Qiao Xinpei Sun Xiaoqiang Tang Jianfang Wang Qiaoran Wang Siyuan Wang Xuan Wang Yaning Wang Yuhan Wang Rimo Wu Kai Xia Fu-Hui Xiao Lingyan Xu Yingying Xu Haoteng Yan Liang Yang Ruici Yang Yuanxin Yang Yilin Ying Le Zhang Weiwei Zhang Wenwan Zhang Xing Zhang Zhuo Zhang Min Zhou Rui Zhou Qingchen Zhu Zhengmao Zhu Feng Cao Zhongwei Cao Piu Chan Chang Chen Guobing Chen Hou-Zao Chen Jun Chen Weimin Ci Bi-Sen Ding Qiurong Ding Feng Gao Jing-Dong JHan Kai Huang Zhenyu Ju Qing-Peng Kong Ji Li Jian Li Xin Li Baohua Liu Feng Liu Lin Liu Qiang Liu Qiang Liu Xingguo Liu Yong Liu Xianghang Luo Shuai Ma Xinran Ma Zhiyong Mao Jing Nie Yaojin Peng Jing Qu Jie Ren Ruibao Ren Moshi Song Zhou Songyang Yi Eve Sun Yu Sun Mei Tian Shusen Wang Si Wang Xia Wang Xiaoning Wang Yan-Jiang Wang Yunfang Wang Catherine CL Wong Andy Peng Xiang Yichuan Xiao Zhengwei Xie Daichao Xu Jing Ye Rui Yue Cuntai Zhang Hongbo Zhang Liang Zhang Weiqi Zhang Yong Zhang Yun-Wu Zhang Zhuohua Zhang Tongbiao Zhao Yuzheng Zhao Dahai Zhu Weiguo Zou Gang Pei Guang-Hui Liu | 2023 | Science China(Life Sciences)2023，66，5： | 2 |
| 14 | Angular spectrum transfer function of photon scanning tunnel microscopy显示文摘 | Zhiyang Li Wu Liu Chuanyun Xiao | 1996 | CCAST WL Workship Series1996，64，： | 1 |
| 15 | HTR2A agonists play a therapeutic role by restricting ILC2 activation in papain-induced lung inflammation显示文摘Group 2 innate lymphoid cells(ILC2s)are a category of heterogeneous cells that produce the cytokines IL-5 and IL-13,which mediate the type 2 immune response.However,specific drug targets on lung ILC2s have rarely been reported.Previous studies have shown that type 2 cytokines,such as IL-5 and IL-13,are related to depression.Here,we demonstrated the negative correlation between the depression-associated monoamine neurotransmitter serotonin and secretion of the cytokines IL-5 and IL-13 by ILC2s in individuals with depression.Interestingly,serotonin ameliorates papain-induced lung inflammation by suppressing ILC2 activation.Our data showed that the serotonin receptor HTR2A was highly expressed on ILC2s from mouse lungs and human PBMCs.Furthermore,an HTR2A selective agonist(DOI)impaired ILC2 activation and alleviated the type 2 immune response in vivo and in vitro.Mice with ILC2-specific depletion of HTR2A(Il5^(cre/+)·Htr2a^(flox/flox)mice)abolished the DOI-mediated inhibition of ILC2s in a papain-induced mouse model of inflammation.In conclusion,serotonin and DOI could restrict the type 2 lung immune response,indicating a potential treatment strategy for type 2 lung inflammation by targeting HTR2A on ST2+ILC2s. | Zhishuo Wang Chenghua Yan Qizhen Du Yuying Huang Xuezhen Li Dan Zeng Ruizhi Mao Rama Krishna Gurram Shipeng Cheng Wangpeng Gu Lin Zhu Weiguo Fan Liyan Ma Zhiyang Ling Ju Qiu Dangsheng Li Enmei Liu Yaguang Zhang Yiru Fang Jinfang Zhu Bing Sun | 2023 | Cellular & Molecular Immunology2023，20，4： | 1 |
| 16 | A Compact Quadrature Coupler Based on Coupled Artificial Transmission Lines显示文摘 | Liu Zhiyang Weikle R M | 2005 | IEEE Microwave and Wireless Components Letters2005，15，： | 1 |
| 17 | Computed tomography has an important role in hollow viscus and mesenteric injuries after blunt abdominal trauma显示文摘 | Ker-Kan Tan Jody Zhiyang Liu | | 0，，05： | 1 |
| 18 | Energetic electron detection packages on board Chinese navigation satellites in MEO显示文摘Energetic electron measurements and spacecraft charging are of great significance for theoretical research in space physics and space weather applications.In this paper,the energetic electron detection package(EEDP)deployed on three Chinese navigation satellites in medium Earth orbit(MEO)is reviewed.The instrument was developed by the space science payload team led by Peking University.The EEDP includes a pinhole medium-energy electron spectrometer(MES),a high-energy electron detector(HED)based onΔE-E telescope technology,and a deep dielectric charging monitor(DDCM).The MES measures the energy spectra of 50−600 keV electrons from nine directions with a 180°×30°field of view(FOV).The HED measures the energy spectrum of 0.5−3.0 MeV electrons from one direction with a 30°cone-angle FOV.The ground test and calibration results indicate that these three sensors exhibit excellent performance.Preliminary observations show that the electron spectra measured by the MES and HED are in good agreement with the results from the magnetic electron-ion spectrometer(MagEIS)of the Van Allen Probes spacecraft,with an average relative deviation of 27.3%for the energy spectra.The charging currents and voltages measured by the DDCM during storms are consistent with the highenergy electron observations of the HED,demonstrating the effectiveness of the DDCM.The observations of the EEDP on board the three MEO satellites can provide important support for theoretical research on the radiation belts and the applications related to space weather. | YuGuang Ye Hong Zou Qiu-Gang Zong HongFei Chen JiQing Zou WeiHong Shi XiangQian Yu WeiYing Zhong YongFu Wang YiXin Hao ZhiYang Liu XiangHong Jia Bo Wang XiaoPing Yang XiaoYun Hao | 2021 | Earth and Planetary Physics2021，5，2： | 1 |
| 19 | Printable microlaser arrays with programmable modes for information encryption显示文摘Lasing emissions with multiple and tunable modes are promising in coding field as a novel cryptographic primitive.With the advantages of simple fabrication,full-color and high-quality-factor whispering gallery mode lasing inside a circular cross-section,polymer microfibers are attractive for photonic devices.However,polymer lasing microfibers for information encryption have never been reported.Herein,we propose a design of printable lasing microfiber encryption chip by in-situ tuning the effective refractive index of the microresonator arrays via a facile approach.Through inkjet printing high-refractive-index nanoparticles on the designated position of lasing microfiber arrays,the effective refractive index of the microcavities is regulated,and the ratio of wavenumber spacing between transverse electric and transverse magnetic mode to the free spectral range can be modulated,particularly with neglectable influence by the size factor.Thus,the programmable region selective encoding process can be conducted simply by a printing program within several minutes.Besides,the encoded microfiber arrays are encapsulated into polydimethylsiloxane to reduce the scattering loss and environmental interference,and a printable encryption chip is realized.This work is expected to provide a platform for the printable encrypted devices. | Jun Ruan Dan Guo Kun Ge Zhiyang Xu Fangyuan Liu Tianrui Zhai | 2023 | Nano Research2023，16，7： | 1 |
| 20 | Surgery for perforated colorectal malignancy in an Asian population: an institution’s experience over 5?years显示文摘 | Ker-Kan Tan Choon-Chiet Hong Junren Zhang Jody Zhiyang Liu Richard Sim | 2010 | International Journal of Colorectal Disease2010，，8： | 1 |
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