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| 1 | KaKs_Calculator 2.0:A Toolkit Incorporating Gamma-Series Methods and Sliding Window Strategies显示文摘We present an integrated stand-alone software package named KaKs_Calculator 2.0 as an updated version.It incorporates 17 methods for the calculation of nonsynonymous and synonymous substitution rates;among them,we added our modified versions of several widely used methods as the gamma series including γ-NG,γ-LWL,γ-MLWL,γ-LPB,γ-MLPB,γ-YN and γ-MYN,which have been demonstrated to perform better under certain conditions than their original forms and are not implemented in the previous version.The package is readily used for the identification of positively selected sites based on a sliding window across the sequences of interests in 5' to 3' direction of protein-coding sequences,and have improved the overall performance on sequence analysis for evolution studies.A toolbox,including C++ and Java source code and executable files on both Windows and Linux platforms together with a user instruction,is downloadable from the website for academic purpose at https://sourceforge.net/projects/kakscalculator2/. | Dapeng Wang Yubin Zhang Zhang Zhang Jiang Zhu Jun Yu | 2010 | Genomics, Proteomics & Bioinformatics2010，8，1： | 79 |
| 2 | Stenting for symptomatic intracranial arterial stenosis in China:1-year outcome of a multicentre registry study显示文摘background and purpose A multicentre prospective registry study of individually tailored stenting for a patient with symptomatic intracranial atherosclerotic stenosis(ICAS)combined with poor collaterals in China showed that the short-term safety and efficacy of stenting was acceptable.However,it remained uncertain whether the low event rate could be of a long term.We reported the 1-year outcome of this registry study to evaluate the long-term efficacy of individually tailored stenting for patients with severe symptomatic ICAS combined with poor collaterals.Methods Patients with symptomatic ICAS caused by 70%-99% stenosis located at the intracranial internal carotid,middle cerebral,intracranial vertebral or basilar arteries combined with poor collaterals were enrolled.Balloon-mounted stent or balloon plus self-expanding stent were selected based on the ease of vascular access and lesion morphology determined by the operators.The primary outcome was the rate of 30-day stroke,transient ischaemic attack and death,and 12-month ischaemic stroke within the same vascular territory,haemorrhagic stroke and vascular death after stenting.results From September 2013 to January 2015,300 patients(ages 58.3±9.78 years)were recruited.Among them,159 patients were treated with balloon-mounted stent and 141 with balloon plus self-expanding stent.During the 1-year follow-up,25 patients had a primary end point event.The probability of primary outcome at 1 year was 8.1%(95% CI 5.3% to 11.7%).In 76 patients with digital subtraction angiography follow-up,27.6%(21/76)had re-stenosis≥50% and 18.4%(14/76)had re-stenosis≥70%.No baseline characteristic was associated with the primary outcome.Conclusion The event rate remains low over 1 year of individually tailored stenting for patients with severe symptomatic ICAS combined with poor collaterals.Further randomised trial of comparing individually tailored stenting with best medical therapy is needed. | Ning Ma Yong Zhang Jie Shuai Changchun Jiang Qiyi Zhu Kangning Chen Li Liu Baomin Li Xiangqun Shi Lianbo Gao Yajie Liu Feng Wang Yongli Li Tieyan Liu Hongbo Zheng Dapeng Mo Feng Gao Yilong Wang Yongjun Wang Lei Feng Zhongrong Miao | 2018 | Stroke & Vascular Neurology2018，3，3： | 29 |
| 3 | 2016 Chinese expert consensus and guidelines for the diagnosis and treatment of papillary thyroid microcarcinoma显示文摘The incidence of papillary thyroid carcinoma(PTC)has exponentially increased in recent years.Papillary thyroid microcarcinoma(PTMC)accounts for the majority of the reported cases of PTC.The debates and crucial issues in PTMC management have received researchers'attention.To further improve the clinical management of PTMC in China, | Ming Gao Minghua Ge Qinghai Ji Ruochuan Cheng Hankui Lu Haixia Guan Li Gao Zhuming Guo Tao Huang Xiaoming Huang Xiaoming Li Yansong Lin Qinjiang Liu Xin Ni Yi Pan Jianwu Qin Zhongyan Shan Hui Sun Xudong Wang Zhengang Xu Yang Yu Daiwei Zhao Naisong Zhang ShengZhang Ying Zheng Jingqiang Zhu Dapeng Li Xiangqian Zheng 无 | 2017 | Cancer Biology & Medicine2017，14，3： | 23 |
| 4 | Initial study of biexponential model of intravoxel incoherent motion magnetic resonance imaging in evaluation of the liver fibrosis显示文摘 | Chen Cuiyun Wang Bin Shi Dapeng Fu Fangfang Zhang Jiliang Wen Zejun Zhu Shaocheng Xu Junling Lin Qing Li Jing Dou Shewei | 2014 | Chinese Medical Journal2014，，17： | 15 |
| 5 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders: executive summary and 2019 update of clinical management of cerebral venous sinus thrombosis显示文摘Aim Cerebral venous sinus thrombosis(CVST)is a less common cerebrovascular disease that predominantly affects young patients.The incidence of CVST is 2-5/10000000/year,accounting for 0.5%-1%of all stroke.To reduce mortality and morbidity associated with CVST,Chinese Stroke Association commissioned the authors to write the current guideline on the management of CVST.Methods PubMed(MEDLINE),CNKI and Wanfang database were searched for studies related to CVST from 1 January 1990 to 31 July 2019.Data were synthesised by evidence tables.Each recommendation was fully discussed by the writing group members and reviewed by Chinese Stroke Association Stroke Fellow Committees.Levels of evidence grading algorithm of Chinese Stroke Association was used to grade each recommendation.results This guideline mainly focuses on the diagnostic evaluation,therapeutic strategies and secondary prevention of CVST.CT/CTV and MRI/MRV are recommended in the initial imaging evaluation of patients with suspected CVST.Anticoagulation therapy with low-molecular weight heparin should be initiated in patients with CVST immediately.After the acute stage,warfarin is recommended for 3-6 months to prevent the recurrence of CVST and other venous thromboembolic events.Conclusions The guideline summarises the current evidence regarding the management of CVST,and provides references for diagnosis,treatment and secondary prevention of CVST in China. | Yuhua Fan Jian Yu Hongbing Chen Jian Zhang Jiangang Duan Dapeng Mo Wenhao Zhu Bo Wang Fubing Ouyang Yicong Chen Linfang Lan Jinsheng Zeng Chinese Stroke Association Stroke Council CVST Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，2： | 14 |
| 6 | Exogenous abscisic acid significantly affects proteome in teaplant (Camellia sinensis) exposed to drought stress显示文摘Tea[Camellia sinensis(L.)O.Kuntze]is an important economic crop,and drought is the most important abiotic stress affecting yield and quality.Abscisic acid(ABA)is an important phytohormone responsible for activating drought resistance.Increased understanding of ABA effects on tea plant under drought stress is essential to develop drought-tolerant tea genotypes,along with crop management practices that can mitigate drought stress.The objective of the present investigation is evaluation of effects of exogenous ABA on the leaf proteome in tea plant exposed to drought stress.Leaf protein patterns of tea plants under simulated drought stress[(polyethylene glycol(PEG)-treated]and exogenous ABA treatment were analyzed in a time-course experiment using two-dimensional electrophoresis(2-DE),followed by matrix-assisted laser desorption/ionization time-of-flight(MALDI-TOF)mass spectrometry(MS).Among the 72 protein spots identified by MALDI-TOF MS,16 proteins were downregulated and two were upregulated by exogenous ABA.The upregulated proteins have roles in glycolysis and photosystem II stabilization.Twenty-one protein spots were responsive to drought stress and most participate in carbohydrate and nitrogen metabolism,control of reactive oxygen species(ROS),defense,signaling or nucleic acid metabolism.The combined treatments of exogenous ABA and drought showed upregulation of 10 protein spots at 12 h and upregulation of 11 proteins at 72 h after initiation of drought stress.The results support the importance of the role that ABA plays in the tea plant during drought stress,by improving protein transport,carbon metabolism and expression of resistance proteins. | Lin Zhou Hui Xu Sue Mischke Lyndel W Meinhardt Dapeng Zhang Xujun Zhu Xinghui Li Wanping Fang | 2014 | Horticulture Research2014，1，1： | 12 |
| 7 | Synthesis of the Diverse Glycosides in Traditional Chinese Medicine显示文摘繁体中文药(TCM ) 是为现代药品的发现和发展的一件巨大的财富藏品。确实,众多的生物学上并且 pharmacologically 重要的分子从 TCM 被识别了;在他们之中,可观的部分属于 glycosides。这些 glycosides 在结构是极其多样的,由 aglycones, glycans,以及 glycosidic 连接的一个大变化组成。另外,这些 glycosides 在自然来源作为同种的物的复杂混合物经常发生。这 microheterogeneity 在可估计的数量导致同类的 glycoside 的隔离一项可怕的任务。因此,向这些自然 glycosides 的合成途径的开发收到了大注意。在这篇报道,我们总结我们在这个话题上花的 twentyyear 努力。55 代表性的 TCMrelevant glycosides 由我们的组综合了被加亮。基于 aglycone 结构,这些 glycosides 被分成三个组,即,类固醇 glycosides 和酉分的 glycosides。典型合成途径被讨论,显示出为 regio 和 glycans 和保护组的全面操作的 stereocontrolled 安装的费力的战术。这些有用、可伸缩的合成途径提供了实际选择给这些 TCM 部件并且这样的可接近性机会为他们的在里面生物的深度和药理学学习。 | Dapeng Zhu Biao Yu | 2018 | Chinese Journal of Chemistry2018，36，8： | 11 |
| 8 | A LysM Receptor Heteromer Mediates Perception of Arbuscular Mycorrhizal Symbiotic Signal in Rice显示文摘Symbiotic microorganisms improve nutrient uptake by plants.To initiate mutualistic symbiosis with arbus-cular mycorrhizal(AM)fungi,plants perceive Myc factors,including lipochitooligosaccharides(LCOs)and short-chain chitooligosaccharides(CO4/CO5),secreted by AM fungi.However,the molecular mechanism of Myc factor perception remains elusive.In this study,we identified a heteromer of LysM receptor-like kinases consisting of OsMYR1/OsLYK2 and OsCERK1 that mediates the perception of AM fungi in rice.CO4 directly binds to OsMYR1,promoting the dimerization and phosphorylation of this receptor complex.Compared with control plants,Osmyr1 and Oscerk1 mutant rice plants are less sensitive to Myc factors and show decreased AM colonization.We engineered transgenic rice by expressing chimeric receptors that respectively replaced the ectodomains of OsMYR1 and OsCERK1 with those from the homologous Nod factor receptors MtNFP and MtL YK3 of Medicago truncatula.Transgenic plants displayed increased cal-cium oscillations in response to Nod factors compared with control rice.Our study provides significant mechanistic insights into AM symbiotic signal perception in rice.Expression of chimeric Nod/Myc recep-tors achieves a potentially important step toward generating cereals that host nitrogen-fixing bacteria. | Jiangman He Chi Zhang Huiling Dai Huan Liu Xiaowei Zhang Jun Yang Xi Chen Yayun Zhu Dapeng Wang Xiaofeng Qi Weichao Li Zhihui Wang Guoyong An Nan YU Zuhua He Yong-Fei Wang Youli Xiao Peng Zhang Ertao Wang | 2019 | Molecular Plant2019，12，12： | 9 |
| 9 | Lead Isotopic Composition and Lead Source in the Bainiuchang Ag-polymetallic Deposit, Yunnan Province, China显示文摘在云南省的 Bainiuchang 存款,中国,在 Gejiu 矿石地和 Dulong 矿石之间地理上被定位地。除了 > 7000 t Ag 储备,存款也与很多驱散的元素炫耀大规模 Pb, Zn 和 Sn 储备(在里面, Cd, Ge, Ga,等等) 。我们系统地决定了存款的 Pb 同位素作文。海地板 exhalative 是在采矿区域的西北的沉积起源的矿石的 Pb 同位素比率,是 206Pb/204Pb = 17.758-18.537 , 207Pb/204Pb = 15.175-15.862 和 208Pb/204Pb = 37.289-39.424 ,当时那些矿石,那具有 magmatic 在采矿区域的东南的热水的重迭起源,分别地是 206Pb/204Pb = 17.264-18.359 , 207Pb/204Pb = 14.843-15.683 和 208Pb/204Pb = 36.481-38.838 。以在 magmatic 岩石或 magmatic 中的长石的 Pb 同位素作文从采矿区域的整个岩石的样品,我们决定了 Pb 同位素作文并且是获得了 Pb 同位素比率:206Pb/204Pb = 18.224-18.700, 207Pb/204Pb = 15.595-15.797 和 208Pb/204Pb = 38.193-39.608。然后,根据 Pb 同位素,变形岩石的作文取样从原生地,地下室在 Dulong 矿石地里暴露了,我们决定了 Pb 同位素作文并且是获得了同位素比率:206Pb/204Pb = 18.434-19.119, 207Pb/204Pb = 15.644-15.693,和 208Pb/204Pb = 38.514-38.832。并且寒武纪的沉积岩石的 Pb 同位素比率,在 Bainiuchang 采矿区域被暴露,是 206Pb/204Pb = 18.307-19.206, 207Pb/204Pb = 15.622-15.809,和 208Pb/204Pb = 38.436-39.932。由关于他们的 Pb 同位素作文比较矿石的二种类型,在 Bainiuchang 存款的铅主要从比在年龄的 Neoproterozoic 早的降低外壳的 granulite 被导出,这被显示,但是热水的液体可能提供了的 Yanshanian magmatic 象为矿石的 Sn 那样的形成矿石的元素的部分在采矿区域的南方堵住。 | ZHU Chaohui ZHANG Qian SHAO Shuxun ZHU Xiaoqing WANG Dapeng | 2008 | Acta Geologica Sinica(English Edition)2008，82，4： | 7 |
| 10 | Sulfur isotopic composition of the Bainiuchang super-large Ag polymetallic deposit,Yunnan Province,China: Implications for the source of sulfur in ore-forming fluids显示文摘The Bainiuchang deposit in Yunnan Province, China, is located geographically between the Gejiu ore field and the Dulong ore field. In addition to >7000 t Ag reserves, the deposit possesses large-scale Pb, Zn, Sn reserves and a mass of dispersed elements (i.e., In, Cd, Ge, Ga, etc.). Based on systematic studies of sulfur isotopic composition, the authors conclude: The Bainiuchang deposit experienced two epochs of metallogenesis, i.e., the Middle-Cambrian sea-floor exhalative sedimentary metallogenic epoch and the Yanshanian magmatic hydrothermal superimposition metallogenic epoch. In the two metallogenic epochs, the δ34S values of sulfides were all near 0, showing a tendency of being enriched slightly in heavy sulfur. The δ34S values of sulfides in the early metallogenic epoch are within the range of 2‰–5‰ with a peak value range of 2‰–3‰ and an average of 3.0‰, and those of sulfides in the late metallogenic epoch are within the range of 2‰–6‰ with a peak value of 3‰–4‰ and an average of 3.9‰. For the single metallogenic epoch, sulfur in the ore-forming fluids in the early epoch already reached isotopic equilibrium and was derived mainly from underneath the magma chamber or basement metamorphic igneous rocks. Sulfur in the sulfides in the late epoch was derived mainly from magmatic hydrothermal fluids formed in the process of remelting of the basement metamorphic igneous rocks. | XIE Hongjing ZHU Chaohui ZHANG Qian WANG Dapeng FAN Liangwu | 2009 | Chinese Journal Of Geochemistry2009，28，3： | 7 |
| 11 | Linear Strain-Gradient Effect on the Energy Bandgap in Bent CdS Nanowires显示文摘尽管在半导体的可能的非同类的紧张效果被调查了因为在一半上世纪和紧张坡度能每在灵活 nanostructures 的测微计是超过 1% ,我们仍然缺乏他们对精力乐队的影响的理解。这里,我们进行紧张坡度的系统的阴极射线发光光谱学研究在在低温度的有弹性地弄弯的 CdS nanowires 的导致的激子精力移动,并且发现在弄弯的 nanowires 的激子精力红移动与紧张坡度成正比,格子失真的一个索引。密度功能的计算在弄弯的 nanostructures 显示出乐队差距减小的一样的趋势并且揭示内在的机制。半导体的乐队差距上的重要线性紧张坡度效果应该在在 nanoelectronics 调节光电子的性质的方法上打开新灯。 | Qiang Fu Zi Yue Zhang Liangzhi KOU Peicai Wu Xiaobing Han Xinli Zhu Jingyun Gao Jun Xu Qing Zhao Wanlin Guo Dapeng Yu | 2011 | Nano Research2011，4，3： | 5 |
| 12 | Sea-floor exhalative sedimentary and magmatic hydrothermal superimposition on the Bainiuchang polymetallic deposit in Yunnan Province: REE geochemical evidence显示文摘In order to study the characteristics of sea-floor exhalative sedimentary and magmatic hydrothermal superimposition on the Bainiuchang polymetallic deposit, the REE compositions of the granites, host-rocks and ores have been systematically analyzed by ICP-MS. As viewed from their REE compositions, the granites show obvious negative Eu anomalies and weak negative Ce anomalies. According to their REE characteristics, the host-rocks were derived partly from sea-floor exhalative sediments. In terms of their REE compositions, the ores can be divided into two groups: one group, of which the samples were collected from the Baiyang segment relatively far away from the Bozhushan granite batholith, possesses positive Eu anomalies or no Eu anomaly and negative Ce anomalies, indicating that ore-forming hydrothermal fluid was relatively reductive and its temperature was higher than 250 ℃. Furthermore, the coinstantaneous presence of positive Eu anomalies and negative Ce anomalies indicate that the convective mixing of a little amount of seawater with hydrothermal fluid had happened while ores were precipitated on ancient sea floor. The other group, of which the samples were mainly collected from the Chuanxindong and Duimenshan segments near the Bozhushan granite batholith, has similar chondrite-monalized REE distribution patterns to those of the magmatic rocks. But as a whole, the REE characteristics of both groups change gradually starting from the Bozhushan granite batholith. Based on the REE characteristics of the granites, host-rocks and ores, it is suggested that the ore-forming metals seem to have come from several different sources. | ZHU Chaohui ZHANG Qian SHAO Shuxun ZHU Xiaoqing HE Yuliang WANG Dapeng | 2007 | Chinese Journal Of Geochemistry2007，26，3： | 4 |
| 13 | Ultra-low V_(pp)and high-modulation-depth InP-based electro-optic microring modulator显示文摘A modulator is an essential building block in the integrated photonics,connecting the electrical with optical signals.The microring modulator gains much attention because of the small footprint,low drive voltage and high extinction ratio.An ultra-low V_(pp)and high-modulation-depth indium phosphide-based racetrack microring modulator is demonstrated in this paper.The proposed device mainly comprises one racetrack microring,incorporating a semiconductor amplifier,and coupling with a bus waveguide through a multimode interference coupler.Traveling wave electrodes are employed to supply bidirectional bias ports,terminating with a 50-Ω impedance.The on/off extinction ratio of the microring reaches 43.3 dB due to the delicately tuning of the gain.An 11 mV V_(pp),a maximum 42.5 dB modulation depth and a 6.6 GHz bandwidth are realized,respectively.This proposed microring modulator could enrich the functionalities and designability of the fundamental integrated devices. | Dapeng Liu Jian Tang Yao Meng Wei Li Ninghua Zhu Ming Li | 2021 | Journal of Semiconductors2021，42，8： | 3 |
| 14 | Numerical and Experimental Study on Contact Face and Shock Wave Motion in the Receiving Tube of Gas Wave Refrigerator显示文摘在煤气的波浪冰箱的一个开的结束收到试管的接触脸和冲击波运动数字地并且试验性地被调查。结果显示出那,当气体在稳定的繁殖以后严厉地被注入收到的试管,和落下,接触脸的速度很快升起。然而,在试管的冲击波的速度是几乎线性的。与入口压力增加,冲击波的速度和接触脸的稳定的速度也增加。另外,在收到的试管的接触脸繁殖的时间和距离变得更长。关键词接触脸 - 冲击波 - 收到试管 - 煤气的波浪冰箱 CLC 数字 O354. | Dapeng HU Shengtao CHEN Hu LIU Zuzhi CHEN Che ZHU | 2006 | Journal of Thermal Science2006，15，4： | 3 |
| 15 | Exo-celiac liver in Glyptosternum maculatum显示文摘A unique structure in the fish of Glyptosternum maculatum(Regan)(Siluriformes:Sisoridae)is reported.It was identified as a part of the liver named 'exo-celiac liver'.This new organ is located between skin and muscle and connected with the celiac liver by a funiform tissue,'joint belt'.It has similar histological features and isozyme electrophoretogramic bands of lactate dehydrogenase,esterase,malate dehydrogenase and alcohol dehydrogenase as in the celiac liver.This unique organ has biological research value and could serve as an important tool for studying organogenesis and evolution. | Li Hongjing Xie Congxin Li Dapeng Chai Yi Liu Hongyan Fan Qixue Zhu Bangke | 2007 | Progress in Natural Science:Materials International2007，17，9： | 3 |
| 16 | Cable-truss supporting system for gob-side entry driving in deep mine and its application显示文摘In order to solve the large deformation controlling problem for surrounding rock of gob-side entry driving under common cable anchor support in deep mine, site survey, physical modeling experiment, numerical simulation and field measurement were synthetically used to analyze the deformation and failure characteristics of surrounding rock. Besides, applicability analysis, prestress field distribution characteristics of surrounding rock and the control effect on large deformation of surrounding rock were also further studied for the gob-side entry driving in deep mine using the cable-truss supporting system. The results show that, first, compared with no support and traditional bolt anchor support, roof cable-truss system can effectively restrain the initiation and propagation of tensile cracks in the roof surrounding rock and arc shear cracks in the two sides, moreover, the broken development of surrounding rock, roof separation and extrusion deformation between the two sides of the roadway are all controlled; second, a prestressed belt of trapezoidal shape is generated in the surrounding rock by the cable-truss supporting system, and the prestress field range is wide. Especially, the prestress concentration belt in the shallow surrounding rock can greatly improve the anchoring strength and deformation resisting capability of the rock stratum;third, an optimized support system of ‘‘roof and side anchor net beam, roof cable-truss supporting system and anchor cable of the narrow coal pillar' was put forward, and the support optimization design and field industrial test were conducted for the gob-side entry driving of the working face 5302 in Tangkou Mine, from which a good supporting effect was obtained. | Yin Qian Jing Hongwen Dai Dapeng Zhu Tantan Zhao Honghui Meng Bo | 2016 | International Journal of Mining Science and Technology2016，26，5： | 3 |
| 17 | Surface Exciton-Plasmon Polariton Enhanced Light Emission via Integration of Single Semiconductor Nanowires with Metal Nanostructures显示文摘The light emission enhancement behavior from single ZnO nanowires integrated with metallic contacts is investigated by micro-photoluminescence measurements.Apart from surface plasmon polaritons at the air/metal interface,the emission of a single ZnO nanowire can be coupled into guided modes of surface exciton-plasmon polaritons(SEPPs).The out-coupling avenues of SEPP guided modes are modeled in the presence of nanostructures,such as corrugation and gratings,on the metal surface.The guided modes of SEPPs in metal-contacted ZnO nanowires are calculated using the effective index method.The enhanced light emission from single semiconductor nanowires shows promise for use in highly efficient nano-emitters and nano-lasers,as well as macroscopic solid state light sources with very high efficiency. | Xuejin Zhang Pengwei Wang Xinzheng Zhang Jun Xu Yongyuan Zhu Dapeng Yu | 2009 | Nano Research2009，2，1： | 2 |
| 18 | Study on gas and wave in a receiving tube显示文摘气体和波浪是在一个收到的试管的运动在现在的纸数字地并且试验性地被调查。结果显示出那,当气体在稳定的繁殖以后严厉地被注入收到的试管,然后落下,接触脸的速度很快升起。然而,在试管的波浪的速度是几乎线性的,波浪能在收到的试管的靠近的结束被反映。与入口压力增加,波浪的速度和接触脸的稳定的速度也增加。当波浪打扫气体,有明显的热效果。反映的波浪能在开的结束加热令人疲乏不堪的气体。作为一个吸收器,在试管罐头的扩展和一位精神病医生几乎完全吸收反映的波浪。 | Dapeng Hu Shengtao Chen Jun Yang Zuzhi Chen Yuqiang Dai Che Zhu Runjie Liu | 2008 | Journal of Thermal Science2008，17，2： | 2 |
| 19 | Development of a CLDN18.2-targeting immuno-PET probe for non-invasive imaging in gastrointestinal tumors显示文摘Claudin18.2(CLDN18.2)is a tight junction protein that is overexpressed in a variety of solid tumors such as gastrointestinal cancer and oesophageal cancer.It has been identified as a promising target and a potential biomarker to diagnose tumor,evaluate efficacy,and determine patient prognosis.TST001 is a recombinant humanized CLDN18.2 antibody that selectively binds to the extracellular loop of human Claudin18.2.In this study,we constructed a solid target radionuclide zirconium-^(89)(^(89)Zr)labled-TST001 to detect the expression of in the human stomach cancer BGC823CLDN18.2 cell lines.The[^(89)Zr]Zr-desferrioxamine(DFO)-TST001 showed high radiochemical purity(RCP,>99%)and specific activity(24.15±1.34 GBq/mmol),and was stable in 5%human serum albumin,and phosphate buffer saline(>85%RCP at 96 h).The EC_(50) values of TST001 and DFO-TST001 were as high as 0.413±0.055 and 0.361±0.058 nM(P>0.05),respectively.The radiotracer had a significantly higher average standard uptake values in CLDN18.2-positive tumors than in CLDN18.2-negative tumors(1.11±0.02 vs.0.49±0.03,P=0.0016)2 days post injection(p.i.).BGC823CLDN18.2 mice models showed high tumor/muscle ratios 96 h p.i.with[^(89)Zr]Zr-DFO-TST001 was much higher than those of the other imaging groups.Immunohistochemistry results showed that BGC823CLDN18.2 tumors were highly positive(+++)for CLDN18.2,while those in the BGC823 group did not express CLDN18.2().The results of ex vivo biodistribution studies showed that there was a higher distribution in the BGC823CLDN18.2 tumor bearing mice(2.05±0.16%ID/g)than BGC823 mice(0.69±0.02%ID/g)and blocking group(0.72±0.02%ID/g).A dosimetry estimation study showed that the effective dose of[^(89)Zr]Zr-DFO-TST001 was 0.0705 mSv/MBq,which is within the range of acceptable doses for nuclear medicine research.Taken together,these results suggest that Good Manufacturing Practices produced by this immuno-positron emission tomography probe can detect CLDN18.2-overexpressing tumors. | Yan Chen Xingguo Hou Dapeng Li Jin Ding Jiayue Liu Zilei Wang Fei Teng Hongjun Li Fan Zhang Yi Gu Steven Yu Xueming Qian Zhi Yang Hua Zhu | 2023 | Journal of Pharmaceutical Analysis2023，13，4： | 2 |
| 20 | How Do Variable Substitution Rates Influence Ka and Ks Calculations?显示文摘The ratio of nonsynonymous substitution rate (Ka) to synonymous substitution rate (Ks) is widely used as an indicator of selective pressure at sequence level among different species, and diverse mutation models have been incorporated into several computing methods. We have previously developed a new γ-MYN method by capturing a key dynamic evolution trait of DNA nucleotide sequences, in consideration of varying mutation rates across sites. We now report a further improvement of NG, LWL, MLWL, LPB, MLPB, and YN methods based on an introduction of gamma distribution to illustrate the variation of raw mutation rate over sites. The novelty comes in two ways: (1) we incorporate an optimal gamma distribution shape parameter a into γ-NG, γ-LWL, γ-MLWL, γ-LPB, γ-MLPB, and γ-YN methods; (2) we investigate how variable substitution rates affect the methods that adopt different models as well as the interplay among four evolutional features with respect to Ka/Ks computations. Our results suggest that variable substitution rates over sites under negative selection exhibit an opposite effect on ω estimates compared with those under positive selection. We believe that the sensitivity of our new methods has been improved than that of their original methods under diverse conditions and it is advantageous to introduce novel parameters for Ka/Ks computation. | Dapeng Wang Song Zhang Fuhong He Jiang Zhu Songnian Hu Jun Yu | 2009 | Genomics, Proteomics & Bioinformatics2009，7，3： | 2 |
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