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| 1 | Homology-mediated end joining-based targeted integration using CRISPR/Cas9显示文摘 | Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang Shi, Linyu Yang, Hui | 2017 | Cell Research2017，27，6： | 28 |
| 2 | The unconformity in Miocene sequence of western Qaidam Basin and its tectonic significance显示文摘An angular unconformity has been identified between the upper and the lower Youshashan Formation based on the study of the regional geology, drillhole, and geophysical data of the Altyn Tagh Mountain foreland, western Qaidam Basin. The distribution of the unconformity, the contacts between the upper and the lower Youshashan Formation, and its relation to the characteristics of the paleo-structure belts suggest a close relationship between the unconformities in the southern flank of the Altyn Tagh Mountain in the west of the Qaidam Basin and the activity of the Altyn Tagh Fault. It was the same time when the Atlyn Tagh Fault Zone was transformed from regional uplifting to rapid-strike-slipping movement during the Miocene, resulting in the cessation of the regional uplift and subsequent massive regional transgression during the deposition of the upper Youshashan Formation, which unconformably overlies (onlaps) the underlying strata of different ages. | WANG Liang XIAO AnCheng GONG QingLin LIU Dong WU Lei ZHOU SuPing SHEN ZhongYan LOU QianQian SUN XiaoWen | 2010 | Science China Earth Sciences2010，53，8： | 10 |
| 3 | Application of a novel inhibitor of human CD59 for the enhancement of complement-dependent cytolysis on cancer cells显示文摘Many monoclonal antibodies(mAbs)have been extensively used in the clinic,such as rituximab to treat lymphoma.However,resistance and non-responsiveness to mAb treatment have been challenging for this line of therapy.Complement is one of the main mediators of antibody-based cancer therapy via the complement-dependent cytolysis(CDC)effect.CD59 plays a critical role in resistance to mAbs through the CDC effect.In this paper,we attempted to investigate whether the novel CD59 inhibitor,recombinant ILYd4,was effective in enhancing the rituximab-mediated CDC effect on rituximab-sensitive RL-7 lymphoma cells and rituximab-induced resistant RR51.2 cells.Meanwhile,the CDC effects,which were mediated by rituximab and anti-CD24 mAb,on the refractory multiple myeloma(MM)cell line ARH-77 and the solid tumor osteosarcoma cell line Saos-2,were respectively investigated.We found that rILYd4 rendered the refractory cells sensitive to the mAb-mediated CDC effect and that rILYd4 exhibited a synergistic effect with the mAb that resulted in tumor cells lysis.This effect on tumor cell lysis was apparent on both hematological tumors and solid tumors.Therefore,rILYd4 may serve as an adjuvant for mAb mediated-tumor immunotherapy. | Tao You Weiguo Hu Xiaowen Ge Jingnan Shen Xuebin Qin | 2011 | Cellular & Molecular Immunology2011，8，2： | 6 |
| 4 | Porous Si/C composite as anode materials for high-performance rechargeable lithium-ion battery显示文摘A novel porous silicon was synthesized through a magnesiothermic reduction method of molecular sieve for the first time, Si/C composite was synthesized by using pitch as carbon source. The porous Si/C composite shows a high initial specific capacity of 2018.5 mAh/g with current density of 0.1 A/g. When the current density increases to 2 A/g, it still exhibits high average specific capacity of 640.3 mAh/g. The porous structure can remit the Si particle pulverization during the lithiation/delithiation process. This article can provide a reference for the research of the porous Si anode for the high performance rechargeable lithium-ion battery. | Bingbing Deng Lian Shen Yangai Liu Tao Yang Manshu Zhang Renjie Liu Zhaohui Huang Minghao Fang Xiaowen Wu | 2017 | Chinese Chemical Letters2017，28，12： | 4 |
| 5 | Temperature-induced Lifshitz transition in topological insulator candidate HfTe_5显示文摘The ongoing discoveries and studies of novel topological quantum materials have become an emergent and important field of condensed matter physics.Recently,HfTe_5 ignited renewed interest as a candidate of a novel topological material.The single-layer HfTe_5 is predicted to be a two-dimensional large band gap topological insulator and can be stacked into a bulk that may host a temperature-driven topological phase transition.Historically,HfTe_5 attracted considerable interest for its anomalous transport properties characterized by a peculiar resistivity peak accompanied by a sign reversal carrier type.The origin of the transport anomaly remains under a hot debate.Here we report the first high-resolution laser-based angle-resolved photoemission measurements on the temperature-dependent electronic structure in HfTe_5.Our results indicated that a temperature-induced Lifshitz transition occurs in HfTe_5,which provides a natural understanding on the origin of the transport anomaly in HfTe_5.In addition,our observations suggest that HfTe_5 is a weak topological insulator that is located at the phase boundary between weak and strong topological insulators at very low temperature. | Yan Zhang Chenlu Wang Guodong Liu Aiji Liang Lingxiao Zhao Jianwei Huang Qiang Gao Bing Shen Jing Liu Cheng Hu Wenjuan Zhao Genfu Chen Xiaowen Jia Li Yu Lin Zhao Shaolong He Fengfeng Zhang Shenjin Zhang Feng Yang Zhimin Wang Qinjun Peng Zuyan Xu Chuangtian Chen Xingjiang Zhou | 2017 | Science Bulletin2017，62，13： | 4 |
| 6 | Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121 in apple显示文摘In plants,the vesicle fusion process plays a vital role in pathogen defence.However,the importance of the vesicle fusion process in apple ring rot has not been studied.Here,we isolated and characterised the apple syntaxin gene MdSYP121.Silencing the MdSYP121 gene in transgenic apple calli increased tolerance to Botryosphaeria dothidea infection;this increased tolerance was correlated with salicylic acid(SA)synthesis-related and signalling-related gene transcription.In contrast,overexpressing MdSYP121 in apple calli resulted in the opposite phenotypes.In addition,the results of RNA sequencing(RNA-Seq)and quantitative real-time PCR(qRT-PCR)assays suggested that MdSYP121 plays an important role in responses to oxidation–reduction reactions.Silencing MdSYP121 in apple calli enhanced the expression levels of reactive oxygen species(ROS)-related genes and the activity of ROS-related enzymes.The enhanced defence response status in MdSYP121-RNAi lines suggests that syntaxins are involved in the defence response to B.dothidea.More importantly,we showed that MdSYP121 forms a soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE)complex with MdSNAP33,and the complex may participate in regulating resistance to B.dothidea.In conclusion,by regulating the interaction of SA pathway and oxidation–reduction process,MdSYP121 can influence the pathogen infection process in apple. | Xiaowen He Yanhong Huo Xiuxia Liu Qianqian Zhou Shouqian Feng Xiang Shen Baohua Li Shujing Wu Xuesen Chen | 2018 | Horticulture Research2018，5，1： | 3 |
| 7 | Development and test of the Export Coefficient Model in the Upper Reach of the Yangtze River显示文摘 | Xiaowen Ding Zhenyao Shen Qian Hong Zhifeng Yang Xing Wu Ruimin Liu | 2009 | Journal of Hydrology2009，，3： | 3 |
| 8 | Effectiveness of intravenous r- tPA versus UK for acute ischaemic stroke: a nationwide prospective Chinese registry study显示文摘Background Intravenous recombinant tissue plasminogen activator(r-tPA)and urokinase(UK)are both recommended for the treatment of acute ischaemic stroke(AIS)in China,but with few comparative outcome data being available.We aimed to compare the outcomes of these two thrombolytic agents for the treatment of patients within 4.5 hours of onset of AIS in routine clinical practice in China.Methods A pre-planned,prospective,nationwide,multicentre,real-world registry of consecutive patients with AIS(age≥18 years)who received r-tPA or UK within 4.5 hours of symptom onset according to local decision-making and guideline recommendations during 2017-2019.The primary effectiveness outcome was the proportion of patients with an excellent functional outcome(defined by modified Rankin scale scores 0 to 1)at 90 days.The key safety endpoint was symptomatic intracranial haemorrhage according to standard definitions.Multivariable logistic regression was used for comparative analysis,with adjustment according to propensity scores to ensure balance in baseline characteristics.Results Overall,4130 patients with AIS were registered but 320 had incomplete or missing data,leaving 3810 with available data for analysis of whom 2666 received r-tPA(median dose 0.88(IQR 0.78-0.90)mg/kg)and 1144 received UK(1.71(1.43-2.00)×104 international unit per kilogram).There were several significant intergroup differences in patient characteristics:r-tPA patients were more educated,had less history of stroke,lower systolic blood pressure,greater neurological impairment and shorter treatment times from symptom onset than UK patients.However,in adjusted analysis,the frequency of excellent outcome(OR 1.18,95%CI 1.00 to 1.40,p=0.052)and symptomatic intracranial haemorrhage(OR 0.70,95%CI 0.33 to 1.47,p=0.344)were similar between groups.Conclusions UK may be as effective and carry a similar safety profile as r-tPA in treating mild to moderate AIS within guidelines in China.Registration http://www.clinicaltrials.gov.unique identifier:NCT02854592. | Xinhong Wang Xiaoqiu Li Yuming Xu Runhui Li Qingcheng Yang Yong Zhao Fengyun Wang Baoying Sheng Runqing Wang Shaoyuan Chen Lihua Wang Liying Shen Xiaowen Hou Yu Cui Duolao Wang Bin Peng Craig S Anderson Huisheng Chen On behalf of INTRECIS Investigators | 2021 | Stroke & Vascular Neurology2021，6，4： | 2 |
| 9 | Mars Orbiter magnetometer of China’s First Mars Mission Tianwen-1显示文摘As one of the seven scientific payloads on board the Tianwen-1 orbiter,the Mars Orbiter Magnetometer(MOMAG)will measure the magnetic fields of and surrounding Mars to study its space environment and the interaction with the solar wind.The instrument consists of two identical triaxial fluxgate magnetometer sensors,mounted on a 3.19 meter-long boom with a seperation of about 90 cm.The dual-magnetometers configuration will help eliminate the magnetic field interference generated by the spacecraft platform and payloads.The sensors are controlled by an electric box mounted inside the orbiter.Each magnetometer measures the ambient vector magnetic field over a wide dynamic range(to 10,000 nT per axis)with a resolution of 1.19 pT.Both magnetometers sample the ambient magnetic field at an intrinsic frequency of 128 Hz,but will operate in a model with alternating frequency between 1 and 32 Hz to meet telemetry allocations. | Kai Liu XinJun Hao YiRen Li TieLong Zhang ZongHao Pan ManMing Chen XiaoWen Hu Xin Li ChengLong Shen YuMing Wang | 2020 | Earth and Planetary Physics2020，4，4： | 2 |
| 10 | Multifunctional microcapsules:A theranostic agent for US/MR/PAT multi-modality imaging and synergistic chemo-photothermal osteosarcoma therapy显示文摘Development of versatile theranostic agents that simultaneously integrate therapeutic and diagnostic features remains a clinical urgent.Herein,we aimed to prepare uniform PEGylated(lactic-co-glycolic acid)(PLGA)microcapsules(PB@(Fe_(3)O_(4)@PEG-PLGA)MCs)with superparamagnetic Fe3O4 nanoparticles embedded in the shell and Prussian blue(PB)NPs inbuilt in the cavity via a premix membrane emulsification(PME)method.On account of the eligible geometry and multiple load capacity,these MCs could be used as efficient multi-modality contrast agents to simultaneously enhance the contrasts of US,MR and PAT imaging.In-built PB NPs furnished the MCs with excellent photothermal conversion property and embedded Fe_(3)O_(4)NPs endowed the magnetic location for fabrication of targeted drug delivery system.Notably,after further in-situ encapsulation of antitumor drug of DOX,(PB+DOX)@(Fe_(3)O_(4)@PEG-PLGA)MCs possessed more unique advantages on achieving near infrared(NIR)-responsive drug delivery and magnetic-guided chemo-photothermal synergistic osteosarcoma therapy.In vitro and in vivo studies revealed these biocompatible(PB+DOX)@(Fe_(3)O_(4)@PEG-PLGA)MCs could effectively target to the tumor tissue with superior therapeutic effect against the invasion of osteosarcoma and alleviation of osteolytic lesions,which will be developed as a smart platform integrating multi-modality imaging capabilities and synergistic effect with high therapy efficacy. | Hufei Wang Sijia Xu Daoyang Fan Xiaowen Geng Guang Zhi Decheng Wu Hong Shen Fei Yang Xiao Zhou Xing Wang | 2022 | Bioactive Materials2022，7，1： | 2 |
| 11 | Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice显示文摘We here report a genome-editing strategy to correct spinal muscular atrophy(SMA).Rather than directly targeting the pathogenic exonic mutations,our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements.We disrupted intronic splicing silencers(ISSs,including ISS-N1 and ISS+100)of survival motor neuron(SMN)2,a key modifier gene of SMA,to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs.Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration.Furthermore,co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9)or Cas9 from Staphylococcus aureus(SaCas9)alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56%and 100%of severe SMA transgenic mice(Smn-/-,SMN2tg/-).The median survival of the resulting mice was extended to>400 days.Collectively,our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases. | Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen | 2020 | National Science Review2020，7，1： | 1 |
| 12 | Long-term variation (1960-2003) and causal factors of non-point-source nitrogen and phosphorus in the upper reach of the Yangtze River显示文摘 | SHEN Zhenyao CHEN Lei DING Xiaowen | 2013 | Journal of Hazardous Materials2013，25，： | 1 |
| 13 | Long-term variation (1960-2003) and causal factors of non-point-source nitrogen and phosphorus in the upper reach of the Yangtze River 显示文摘 | SHEN Zhenyao CHEN Lei DING Xiaowen | 2013 | Journal of Hazardous Materials2013，25，： | 1 |
| 14 | Development and test of the export coefficient model in the upper reach of the yangtze river显示文摘 | Ding Xiaowen Shen Zhenyao Hong Qian | 2010 | Journal of Hydrology2010，383，： | 1 |
| 15 | Development and test of the export coefficient model in the upper reach of the Yangtze River显示文摘 | Ding Xiaowen Shen Zhenyao Hong Qian | 2010 | Journal of Hydrology2010，383，3： | 1 |
| 16 | Development and test of the Export Coefficient Model in the Upper Reach of the Yangtze River 显示文摘 | DING Xiaowen SHEN Zhenyao HONG Qian | 2010 | Journal of Hydrology2010，383，： | 1 |
| 17 | An engineered xCas12i with high activity,high specificity,and broad PAM range显示文摘Dear Editor,The clustered regularly interspaced short palindromic repeats-Cas(CRISPR-Cas)systems,including type II Cas9 and type V Cas12 systems,which serve in the adaptive immunity of prokaryotes against viruses,have been developed into genome-editing tools(Anzalone et al.,2020;Doudna,2020).Compared with type II systems,the type V systems including V-A to V-K showed more functional diversity(Yan et al.,2019).Amongst them,Cas12i has a relatively smaller size(1,033-1,093 aa),compared to SpCas9 and Cas12a,and has a 5'-TTN protospacer adjacent motif(PAM)preference(Yan et al.,2019). | Hainan Zhang Xiangfeng Kong Mingxing Xue Jing Hu Zikang Wang Yinghui Wei Haoqiang Wang Jingxing Zhou Weihong Zhang Mengqiu Xu Xiaowen Shen Fengcai Yin Zhiyuan Ai Guangyan Huang Junhui Xia Xueqiong Song Hengbin Li Yuan Yuan Jinhui Li Na Zhong Meiling Zhang Yingsi Zhou Hui Yang | 2023 | Protein & Cell2023，14，7： | 0 |
| 18 | Geological characteristics and models of fault-foldfracture body in deep tight sandstone of the second member of Upper Triassic Xujiahe Formation in Xinchang structural belt of Sichuan Basin,SW China显示文摘In the second member of the Upper Triassic Xujiahe Formation(T_(3)x_(2))in the Xinchang area,western Sichuan Basin,only a low percent of reserves has been recovered,and the geological model of gas reservoir sweet spot remains unclear.Based on a large number of core,field outcrop,test and logging-seismic data,the T_(3)x_(2) gas reservoir in the Xinchang area is examined.The concept of fault-fold-fracture body(FFFB)is proposed,and its types are recognized.The main factors controlling fracture development are identified,and the geological models of FFFB are established.FFFB refers to faults,folds and associated fractures reservoirs.According to the characteristics and genesis,FFFBs can be divided into three types:fault-fracture body,fold-fracture body,and fault-fold body.In the hanging wall of the fault,the closer to the fault,the more developed the effective fractures;the greater the fold amplitude and the closer to the fold hinge plane,the more developed the effective fractures.Two types of geological models of FFFB are established:fault-fold fracture,and matrix storage and permeability.The former can be divided into two subtypes:network fracture,and single structural fracture,and the later can be divided into three subtypes:bedding fracture,low permeability pore,and extremely low permeability pore.The process for evaluating favorable FFFB zones was formed to define favorable development targets and support the well deployment for purpose of high production.The study results provide a reference for the exploration and development of deep tight sandstone oil and gas reservoirs in China. | LIU Junlong LIU Zhongqun LIU Zhenfeng LIU Yali SHEN Baojian XIAO Kaihua BI Youyi WANG Xiaowen WANG Ail FAN Lingxiao LI Jitongl | 2023 | Petroleum Exploration and Development2023，50，3： | 0 |
| 19 | Venous thromboembolism in children with acute lymphoblastic leukemia in China:a report from the Chinese Children’s Cancer Group-ALL-2015显示文摘Venous thromboembolism(VTE)is a complication in children with acute lymphoblastic leukemia(ALL).The Chinese Children’s Cancer Group-ALL-2015 protocol was carried out in China,and epidemiology,clinical characteristics,and risk factors associated with VTE were analyzed.We collected data on VTE in a multiinstitutional clinical study of 7640 patients with ALL diagnosed in 20 hospitals from January 2015 to December 2019.First,VTE occurred in 159(2.08%)patients,including 90(56.6%)during induction therapy and 108(67.92%)in the upper extremities.T-ALL had a 1.74-fold increased risk of VTE(95%CI 1.08–2.8,P=0.022).Septicemia,as an adverse event of ALL treatment,can significantly promote the occurrence of VTE(P<0.001).Catheter-related thrombosis(CRT)accounted for 75.47%(n=120);and,symptomatic VTE,58.49%(n=93),which was more common in patients aged 12–18 years(P=0.023),non-CRT patients(P<0.001),or patients with cerebral thrombosis(P<0.001).Of the patients with VTE treated with anticoagulation therapy(n=147),4.08%(n=6)had bleeding.The VTE recurrence rate was 5.03%(n=8).Patients with VTE treated by non-ultrasoundguided venous cannulation(P=0.02),with residual thrombus(P=0.006),or with short anticoagulation period(P=0.026)had high recurrence rates.Thus,preventing repeated venous puncture and appropriately prolonged anticoagulation time can reduce the risk of VTE recurrence. | Mengmeng Yin Hongsheng Wang Xianmin Guan Ju Gao Minghua Yang Ningling Wang Tianfeng Liu Jingyan Tang Alex WK Leung Fen Zhou Xuedong Wu Jie Huang Hong Li Shaoyan Hu Xin Tian Hua Jiang Jiaoyang Cai Xiaowen Zhai Shuhong Shen Qun Hu | 2023 | Frontiers of Medicine2023，17，3： | 0 |
| 20 | Genetic Architecture of Childhood Kidney and Urological Diseases in China显示文摘Kidney disease is manifested in a wide variety of phenotypes,many of which have an important hereditary component.To delineate the genotypic and phenotypic spectrum of pediatric nephropathy,a multicenter registration system is being imple-mented based on the Chinese Children Genetic Kidney Disease Database(CCGKDD).In this study,all the patients with kidney and urological diseases were recruited from 2014 to 2020.Genetic analysis was conducted using exome sequencing for families with multiple affected individuals with nephropathy or clinical suspicion of a genetic kidney disease owing to early-onset or extrarenal features.The genetic diagnosis was confirmed in 883 of 2256(39.1%)patients from 23 provinces in China.Phenotypic profiles showed that the primary diagnosis included steroid-resistant nephrotic syndrome(SRNS,23.5%),glomerulonephritis(GN,32.2%),congenital anomalies of the kidney and urinary tract(CAKUT,21.2%),cystic renal disease(3.9%),renal calcinosis/stone(3.6%),tubulopathy(9.7%),and chronic kidney disease of unknown etiology(CKDu,5.8%).The pathogenic variants of 105 monogenetic disorders were identified.Ten distinct genomic disorders were identified as pathogenic copy number variants(CNVs)in 11 patients.The diagnostic yield differed by subgroups,and was highest in those with cystic renal disease(66.3%),followed by tubulopathy(58.4%),GN(57.7%),CKDu(43.5%),SRNS(29.2%),renal calcinosis/stone(29.3%)and CAKUT(8.6%).Reverse phenotyping permitted correct identification in 40 cases with clinical reassessment and unexpected genetic conditions.We present the results of the largest cohort of children with kidney disease in China where diagnostic exome sequencing was performed.Our data demonstrate the utility of family-based exome sequencing,and indicate that the combined analysis of genotype and phenotype based on the national patient registry is pivotal to the genetic diagnosis of kidney disease. | Ye Fang Hua Shi Tianchao Xiang Jiaojiao Liu Jialu Liu Xiaoshan Tang Xiaoyan Fang Jing Chen Yihui Zhai Qian Shen Guomin Li Li Sun Yunli Bi Xiang Wang Yanyan Qian Bingbing Wu Huijun Wang Wenhao Zhou Duan Ma Jianhua Mao Xiaoyun Jiang Shuzhen Sun Ying Shen Xiaorong Liu Aihua Zhang Xiaowen Wang Wenyan Huang Qiu Li Mo Wang Xiaojie Gao Yubin Wu Fang Deng Ruifeng Zhang Cuihua Liu Li Yu Jieqiu Zhuang Qing Sun Xiqiang Dang Haitao Bai Ying Zhu Siguang Lu Bili Zhang Xiaoshan Shao Xuemei Liu Mei Han Lijun Zhao Yuling Liu Jian Gao Ying Bao Dongfeng Zhang Qingshan Ma Liping Zhao Zhengkun Xia Biao Lu Yulong Wang Mengzhun Zhao Jianjiang Zhang Shan Jian Guohua He Huifeng Zhang Bo Zhao Xiaohua LI Feiyan Wang Yufeng Li Hongtao Zhu Xinhui Luo Jinghai Li Jia Rao Hong Xu | 2021 | Phenomics2021，1，3： | 0 |
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