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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis显示文摘 | Yao Chen Yuxuan Zheng Yun Gao Zhen Lin Suming Yang Tongtong Wang Qiu Wang Nannan Xie Rong Hua Mingxi Liu Jiahao Sha Michael D. Griswold Jinsong Li Fuchou Tang Ming-Han Tong | 2018 | Cell Research2018，28，9： | 26 |
| 3 | Small molecule inhibitors reveal allosteric regulation of USP14 via steric blockade显示文摘The ubiquitin system is important for drug discovery,and the discovery of selective small-molecule inhibitors of deubiquitinating enzymes (DUBs) remains an active yet extremely challenging task. With a few exceptions,previously developed inhibitors have been found to bind the evolutionarily conserved catalytic centers of DUBs,resulting in poor selectivity. The small molecule IU1 was the first-ever specific inhibitor identified and exhibited surprisingly excellent selectivity for USP14 over other DUBs. However,the molecular mechanism for this selectivity was elusive. Herein,we report the high-resolution co-crystal structures of the catalytic domain of USP14 bound to IU1 and three IU1 derivatives. All the structures of these complexes indicate that IU1 and its analogs bind to a previously unknown steric binding site in USP14,thus blocking the access of the C-terminus of ubiquitin to the active site of USP14 and abrogating USP14 activity. Importantly,this steric site in USP14 is very unique,as suggested by structural alignments of USP14 with several known DUB X-ray structures. These results,in conjunction with biochemical characterization,Indicate a coherent steric blockade mechanism for U5P14 inhibition by compounds of the IU series. In light of the recent report of steric blockade of USP7 by FT671,this work suggests a potential generally applicable allosteric mechanism for the regulation of DUBs via steric blockade,as showcased by our discovery of IU1-248 which is 10-fold more potent than IU1. | Yiwei Wang Yuxuan Jiang Shan Ding Jiawang Li Ningjing Song Yujing Ren Danning Hong Cai Wu Bin Li Feng Wang Wei He Jiawei Wang Ziqing Mei | 2018 | Cell Research2018，28，12： | 15 |
| 4 | Attribution of PM_(2.5) exposure in Beijing-Tianjin-Hebei region to emissions:implication to control strategies显示文摘The Beijing-Tianjin-Hebei(BTH) region is one of the most heavily polluted regions in China,with both high PM_(2.5) concentrations and a high population density.A quantitative source-receptor relationship can provide valuable insights that can inform effective emission control strategies.Both source apportionment(SA) and source sensitivity(SS) can provide such information from different perspectives.In this study,both methods are applied in northern China to identify the most significant emission categories and source regions for PM_(2.5) exposure in BTH in 2013.Despite their differences,both models show similar distribution patterns for population and simulated PM_(2.5) concentrations,resulting in overall high PM_(2.5) exposure values(approximately 110 μg/m~) and particularly high exposure values during the winter(approximately 200 μ/m~).Both methods show that local emissions play a dominant role(70%),with some contribution from surrounding provinces(e.g.,Shandong) via regional transport.The two methods also agree on the priority of local emission controls:both identify industrial,residential,and agricultural emissions as the top three categories that should be controlled locally.In addition,the effect of controlling agricultural ammonia emissions is approximately doubled when the co-benefits of reducing nitrate are considered.The synthesis of SA and SS for addressing specific categories of emissions provides a quantitative basis for the development of emission control strategies and policies for controlling PM_(2.5)in China. | Xin Li Qiang Zhang Yang Zhang Lin Zhang Yuxuan Wang Qianqian Zhang Meng Li Yixuan Zheng Guannan Geng Timothy J.Wallington Weijian Han Wei Shen Kebin He | 2017 | Science Bulletin2017，62，13： | 15 |
| 5 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 6 | The Low Energy X-ray telescope (LE) onboard the Insight-HXMT astronomy satellite显示文摘The Low Energy X-ray telescope(LE) is one of the three main instruments of the Insight-Hard X-ray Modulation Telescope(Insight-HXMT). It is equipped with Swept Charge Device(SCD) sensor arrays with a total geometrical area of 384 cm^2 and an energy band from 0.7 to 13 ke V. In order to evaluate the particle induced X-ray background and the cosmic X-ray background simultaneously, LE adopts collimators to define four types of Field Of Views(FOVs), i.e., 1.6°×6°, 4°×6°, 50°-60°×2°-6 oand the blocked ones which block the X-ray by an aluminum cover. LE is constituted of three detector boxes(LEDs) and an electric control box(LEB) and achieves a good energy resolution of 140 e V@5.9 ke V, an excellent time resolution of 0.98 ms, as well as an extremely low pileup(<1%@18000 cts/s). Detailed performance tests and calibration on the ground have been performed,including energy-channel relation, energy response, detection efficiency and time response. | Yong Chen WeiWei Cui Wei Li Juan Wang YuPeng Xu FangJun Lul YuSa Wang TianXiang Chen DaWei Han Wei Hu Yi Zhang Jia Huo YanJi Yang MaoShun Li Bo Lu ZiLiang Zhang TiPei Li ShuangNan Zhang ShaoLin Xiong Shu Zhang RongFeng Xue XiaoFan Zhao Yue Zhu YuXuan Zhu HongWei Liu YiJung Yang Fan Zhang | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，4： | 8 |
| 7 | Influence of the intensity and loading time of direct current electric field on the directional migration of rat bone marrow mesenchymal stem cells显示文摘Exogenic 电场能有效地加速骨头愈合并且向受伤区域通过骨头髓的提高的迁居改变间充质的干细胞(BMSC ) 。这研究试图决定下列:(1 ) 在到一个直接当前的电场(DCEF ) 的暴露之上的老鼠 BMSC (rBMSC ) 移植的方向,(2 ) 最佳的 DCEF 紧张和持续时间,并且(3 ) 在由 DCEF 导致了的 rBMSC 移植的 SDF-1/CXCR4 轴的可能的规章的角色。显示出的结果 rBMSCs 移居了到 DCEF 的积极电极,并且为 4 h 的 200 mV/mm 的 DCEF 被发现在提高 rBMSC 移植最佳。这 DCEF 力量和持续时间另外 upregulated osteoblastic 基因,包括的高山和 OCN 的表示,和 upregulated 高山和 Runx2 蛋白质的表示。而且当 CXCR4 被禁止时, rBMSC 移植部分由于 DCEF 被堵住。这些调查结果显示 DCEF 能有效地导致 rBMSC 移植。因为它支持 rBMSC 移植,增长,和 osteogenic 区别的能力,为 4 h 的 200 mV/mm 的 DCEF 被推荐。表明小径的 SDF-1/CXCR4 可以在调整 rBMSCs 的导致 DCEF 的迁居起一个重要作用。 | Xiaoyu Wang Yuxuan Gao Haigang Shi Na Liu Wei Zhang Hongbo Li | 2016 | Frontiers of Medicine2016，10，3： | 7 |
| 8 | Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors显示文摘Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells(PSCs)is a central aim in T cell regenerative medicine.To date,preferentially reconstituting T lymphopoiesis in vivo from PSCs remains a practical challenge.Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial-to-hematopoietic transition and hematopoietic maturation stages in a PSC differentiation scheme(iR9-PSC)in vitro induced preferential generation of engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T cells in primary and secondary immunodeficient recipients.Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSCs,unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors.The induced T cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβrepertoire.The regenerative T lymphopoiesis restored immune surveillance in immunodeficient mice.Furthermore,gene-edited iR9-PSCs produced tumor-specific T cells in vivo that effectively eradicated tumor cells.This study provides insight into universal generation of functional and therapeutic T cells from the unlimited and editable PSC source. | Rongqun Guo Fangxiao Hu Qitong Weng Cui Lv Hongling Wu Lijuan Liu Zongcheng Li Yang Zeng Zhijie Bai Mengyun Zhang Yuting Liu Xiaofei Liu Chengxiang Xia Tongjie Wang Peiqing Zhou Kaitao Wang Yong Dong Yuxuan Luo Xiangzhong Zhang Yuxian Guan Yang Geng Juan Du Yangqiu Li Yu Lan Jiekai Chen Bing Liu Jinyong Wang | 2020 | Cell Research2020，30，1： | 7 |
| 9 | Wide-steering-angle high-resolution optical phased array显示文摘Optical phased array(OPA)technology is considered a promising solution for solid-state beam steering to supersede the traditional mechanical beam steering.As a key component of the LIDAR system for long-range detection,OPAs featuring a wide steering angle and high resolution without beam aliasing are highly desired.However,a wide steering range requires a waveguide pitch less than half of the wavelength,which is easily subjected to cross talk.Besides,high resolution requires a large aperture,and it is normally achieved by a high count number of waveguides,which complicates the control system.To solve the mentioned issues,we design two high-performance 128-channel OPAs fabricated on a multilayered SiN-on-SOI platform.Attributed to the nonuniform antenna pitch,only 128 waveguides are used to achieve a 4 mm wide aperture.Besides,by virtue of innovative dual-level silicon nitride(Si_(3)N_(4))waveguide grating antennas,the fishbone antenna OPA achieves a 100°×19.4°field of view(FOV)with divergence of 0.021°×0.029°,and the chain antenna OPA realizes a 140°×19.23°FOV with divergence of 0.021°×0.1°.To our best knowledge,140°is the widest lateral steering range in two-dimensional OPA,and 0.029°is the smallest longitudinal divergence.Finally,we embed the OPA into a frequency-modulated continuous-wave system to achieve 100 m distance measurement.The reflected signal from 100 m distance is well detected with 26 dBm input transmitter power,which proves that OPA serves as a promising candidate for transceiving optical signal in a LIDAR system. | YINGZHI LI BAISONG CHEN QUANXIN NA QIJIE XIE MIN TAO LANXUAN ZHANG ZIHAO ZHI YUXUAN LI XIAOBIN LIU XIANSHU LUO GUOQIANG LO FENGLI GAO XUEYAN LI JUNFENG SONG | 2021 | Photonics Research2021，9，12： | 7 |
| 10 | Predictive value of immune cell counts and neutrophil-to-lymphocyte ratio for 28-day mortality in patients with sepsis caused by intra-abdominal infection显示文摘Background:The current study aimed to evaluate the value of immune cell counts and neutrophilto-lymphocyte ratio(NLR)when attempting to predict 28-day mortality.Methods:We conducted an observational retrospective study that included consecutive septic patients.Severity scores on the first day and peripheral circulating immune cell counts(at day 1,day 3,day 5 and day 7 of admission)were collected during each patient’s emergency intensive care unit stay.We assessed the associations of peripheral circulating immune cell counts and NLR with the severity of illness.The relationships between 28-day mortality and peripheral circulating immune cell counts and NLR with were evaluated using Cox proportional cause-specific hazards models.Results:A total of 216 patients diagnosed with sepsis caused by IAI were enrolled.The lymphocyte counts(days 1,3,5 and 7)and monocyte counts(days 3,5 and 7)were significantly lower in nonsurvivors(n=72)than survivors(n=144).The NLR values at each time point were significantly higher in non-survivors.The day 1 lymphocyte counts,as well as the monocyte counts,were significantly lower in the highest-scoring group,when stratified by the Acute Physiology and Chronic Health Evaluation II and Sequential Organ Failure Assessment scores,than in the other groups(p<0.05).The day 1 NLR was significantly higher in the highest-scoring group than in the other groups(p<0.05).The day 5 and day 7 lymphocyte counts,day 3 and day 7 monocyte counts and day 7 NLR were significant predictors of 28-day mortality in the Cox proportional hazards models(day 5 lymphocyte count:hazard ratio,0.123(95%CI,0.055–0.279),p<0.001;day 7 lymphocyte count:hazard ratio,0.115(95%CI,0.052–0.254),p<0.001;day 3 monocyte count:hazard ratio,0.067(95%CI,0.005–0.861),p=0.038;day 7 monocyte count:hazard ratio,0.015(95%CI,0.001–0.158),p<0.001;day 7 NLR:hazard ratio,0.773(95%CI,0.659–0.905),p=0.001).Conclusions The results showed that circulating lymphocytes and monocytes were dramatically decreased within 7 days in non-survivors following sepsis from an IAI.Lymphocyte counts,monocyte counts and NLR appeared to be associated with the severity of illness,and they may serve as independent predictors of 28-day mortality in septic patients with IAIs. | Shuangqing Liu Yuxuan Li Fei She Xiaodong Zhao Yongming Yao | 2021 | Burns & Trauma2021，9，1： | 6 |
| 11 | Design and baseline data of a populationbased metabonomics study of eye diseases in eastern China: the Yueqing Ocular Diseases Investigation显示文摘Background:China is undergoing a massive transition toward an urban and industrial economy.These changes will restructure the demographics and economy which will eventually influence the future patterns of disease.The risk factors of vision-impairing eye diseases remain ambiguous and poorly understood.Metabolomics is an ideal tool to understand and shed light on the ocular disease mechanisms for earlier treatment.This article aims to describe the design,methodology and baseline data of the Yueqing Ocular Diseases Investigation(YODI),a developed county population-based study to determine the prevalence and primary causes of visual impairment;also with metabonomics analysis we aimed to identify,predict and suggest some preventive biomarkers that cause blindness.Methods:A population-based,cross-sectional study.Randomized clustering sampling was used to identify adults aged 50 years and older in Xiangyang Town,Yueqing county-level City.The interviews covered demographic,behavioral,ocular risk factors and mental health state.The ocular examination included visual acuity,autorefraction,intraocular pressure,anterior and posterior segment examinations,fundus photography,retinal tomography and angiography,and visual field testing.Anthropometric measurements included height and weight,waist and hip circumference,blood pressure,pulse rate,electrocardiogram,and abdominal ultrasound scan.A venous blood sample was collected for laboratory tests and metabonomics studies.Results:Of the 5319 individuals recruited for the YODI,4769(89.7%)subjects were enrolled for analyses.The median age was 62.0 years,and 45.6%were male.The educational level of illiteracy or semi-illiteracy,primary,middle and high school or above was 29.8%,45.5%,20.1%,and 3.3%,respectively.Majority of the participants were female,younger,and less educated when compared with nonparticipants.The average body mass index and waist-hip ratios were 24.4±3.4 kg/m^(2) and 0.9±0.1 respectively.Blood sample collection reached a sample size of 1909(479 from subjects with selfreported diabetes and 1430 from one-third of the 4290 subjects without self-reported diabetes).Conclusions:The YODI provides population-based data with a high response rate(89.7%)on the prevalence and primary causes of major vision-impairing eye diseases in developed county areas in eastern China.Metabonomics analysis from YODI will provide further association of metabolic characteristics with the visual impairment eye diseases.The risk prediction model could be created and has the potential to be generalized to developed eastern areas in China for prevention. | Yuxuan Deng Yuanbo Liang Sigeng Lin Liang Wen Jin Li Yue Zhou Meixiao Shen Jingwei Zheng Kemi Feng Yanting Sun Kwapong Willaim Robert Jia Qu Fan Lu | 2020 | Eye and Vision2020，7，1： | 4 |
| 12 | The landscape of aging显示文摘Aging is characterized by a progressive deterioration of physiological integrity,leading to impaired functional ability and ultimately increased susceptibility to death.It is a major risk factor for chronic human diseases,including cardiovascular disease,diabetes,neurological degeneration,and cancer.Therefore,the growing emphasis on “healthy aging” raises a series of important questions in life and social sciences.In recent years,there has been unprecedented progress in aging research,particularly the discovery that the rate of aging is at least partly controlled by evolutionarily conserved genetic pathways and biological processes.In an attempt to bring full-fledged understanding to both the aging process and age-associated diseases,we review the descriptive,conceptual,and interventive aspects of the landscape of aging composed of a number of layers at the cellular,tissue,organ,organ system,and organismal levels. | Yusheng Cai Wei Song Jiaming Li Ying Jing Chuqian Liang Liyuan Zhang Xia Zhang Wenhui Zhang Beibei Liu Yongpan An Jingyi Li Baixue Tang Siyu Pei Xueying Wu Yuxuan Liu Cheng-Le Zhuang Yilin Ying Xuefeng Dou Yu Chen Fu-Hui Xiao Dingfeng Li Ruici Yang Ya Zhao Yang Wang Lihui Wang Yujing Li Shuai Ma Si Wang Xiaoyuan Song Jie Ren Liang Zhang Jun Wang Weiqi Zhang Zhengwei Xie Jing Qu Jianwei Wang Yichuan Xiao Ye Tian Gelin Wang Ping Hu Jing Ye Yu Sun Zhiyong Mao Qing-Peng Kong Qiang Liu Weiguo Zou Xiao-Li Tian Zhi-Xiong Xiao Yong Liu Jun-Ping Liu Moshi Song Jing-Dong J.Han Guang-Hui Liu | 2022 | Science China(Life Sciences)2022，65，12： | 4 |
| 13 | Deciphering primate retinal aging at single-cell resolution显示文摘Dear Editor, The retina is a light-sensitive highly-organized tissue,which is vulnerable to aging and age-related retinal diseases.Specifically,progressive retinal degeneration leads to visual function deterioration and vision impairment in the elderly(Lin et al.,2016).In diseases such as age-related macular degeneration(AMD),retinitis pigmentosa(RP)and diabetic retinopathy(DR),pathological process lacking effective treatments profoundly and negatively impact on the quality of life in the elderly(Lin et al.,2016;Chen et al.,2019).Thus,an in-depth molecular assessment of the mechanisms driv-ing retinal aging is of urgent scientific and medical importance. | Si Wang Yuxuan Zheng Qingqing Li Xiaojuan He Ruotong Ren Weiqi Zhang Moshi Song Huifang Hu Feifei Liu Guoqiang Sun Shuhui Sun Zunpeng Liu Yang Yu Piu Chan Guo-Guang Zhao Qi Zhou Guang-Hui Liu Fuchou Tang Jing Qu | 2021 | Protein & Cell2021，12，11： | 4 |
| 14 | History,progress and future challenges of artificial blood vessels:a narrative review显示文摘Cardiovascular disease serves as the leading cause of death worldwide,with stenosis,occlusion,or severe dysfunction of blood vessels being its pathophysiological mechanism.Vascular replacement is the preferred surgical option for treating obstructed vascular structures.Due to the limited availability of healthy autologous vessels as well as the incidence of postoperative complications,there is an increasing demand for artificial blood vessels.From synthetic to natural,or a mixture of these components,numerous materials have been used to prepare artificial vascular grafts.Although synthetic grafts are more appropriate for use in medium to large-diameter vessels,they fail when replacing small-diameter vessels.Tissue-engineered vascular grafts are very likely to be an ideal alternative to autologous grafts in small-diameter vessels and are worthy of further investigation.However,a multitude of problems remain that must be resolved before they can be used in biomedical applications.Accordingly,this review attempts to describe these problems and provide a discussion of the generation of artificial blood vessels.In addition,we deliberate on current state-of-the-art technologies for creating artificial blood vessels,including advances in materials,fabrication techniques,various methods of surface modification,as well as preclinical and clinical applications.Furthermore,the evaluation of grafts both in vivo and in vitro,mechanical properties,challenges,and directions for further research are also discussed. | Ke Hu Yuxuan Li Zunxiang Ke Hongjun Yang Chanjun Lu Yiqing Li Yi Guo Weici Wang | 2022 | Biomaterials Translational2022，3，1： | 3 |
| 15 | Infection and Colonization of Pathogenic Fungus Fusarium proliferatum in Rice Spikelet Rot Disease显示文摘Rice spikelet rot disease(RSRD), caused by Fusarium proliferatum, is an emerging disease. So far, the effects of diseased rice floral organs as well as the primary infection sites and stages of this pathogen are not determined. We investigated changes in the floral organs, along with the infection processes of the pathogen in plants inoculated with F. proliferatum and labelled with a green fluorescent protein during different growth stages of rice. The results showed that RSRD is not a systemic infectious disease, which has negative effects on the fertility of the infected rice. F. proliferatum caused brown colored anthers, crinkled pistils and ovaries, pollen grain deformities and anther indehiscence. The number of pollen grains on the stigmas decreased significantly in the infected spikelets, and the anther dehiscence and seed-setting rate successively declined by 69% and 73%, respectively, as a result of the infection. The initial infection stage occurred at the pollen cell maturity stage, and the primary invasion sites were determined to be the anthers of rice. It was noted that the pathogen mainly damaged the pollen cells, and with the exception of the filaments, proceeded to colonize the pistils and endosperm. | SUN Lei WANG Ling LIU Lianmeng HOU Yuxuan XU Yihua LIANG Mengqi GAO Jian LI Qiqin HUANG Shiwen | 2019 | Rice science2019，26，1： | 3 |
| 16 | A Monte Carlo Neural Fictitious Self-Play approach to approximate Nash Equilibrium in imperfect-information dynamic games显示文摘Solving the optimization problem to approach a Nash Equilibrium point plays an important role in imperfect information games,e.g.,StarCraft and poker.Neural Fictitious Self-Play(NFSP)is an effective algorithm that learns approximate Nash Equilibrium of imperfect-information games from purely self-play without prior domain knowledge.However,it needs to train a neural network in an off-policy manner to approximate the action values.For games with large search spaces,the training may suffer from unnecessary exploration and sometimes fails to converge.In this paper,we propose a new Neural Fictitious Self-Play algorithm that combines Monte Carlo tree search with NFSP,called MC-NFSP,to improve the performance in real-time zero-sum imperfect-information games.With experiments and empirical analysis,we demonstrate that the proposed MC-NFSP algorithm can approximate Nash Equilibrium in games with large-scale search depth while the NFSP can not.Furthermore,we develop an Asynchronous Neural Fictitious Self-Play framework(ANFSP).It uses asynchronous and parallel architecture to collect game experience and improve both the training efficiency and policy quality.The experiments with th e games with hidden state information(Texas Hold^m),and the FPS(firstperson shooter)games demonstrate effectiveness of our algorithms. | Li ZHANG Yuxuan CHEN Wei WANG Ziliang HAN Shijian Li Zhijie PAN Gang PAN | 2021 | Frontiers of Computer Science2021，15，5： | 3 |
| 17 | A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2显示文摘The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus,and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.On the other hand,several concerning SARS-CoV-2 variants,including Omicron,are required to be identified as soon as the samples are identified as‘positive’.Unfortunately,a traditional PCR test does not allow their specific identification.Herein,for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing),which combines an optical sensing technology-surface plasmon resonance(SPR)with the‘gene scissors’clustered regularly interspaced short palindromic repeat(CRISPR)technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants.MOPCS is a low-cost,CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA,without the need for amplification,within 38 min from sample input to results output,and achieve a limit of detection of 15 fM.MOPCS achieves a highly sensitive analysis of SARS-CoV-2,and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron)and BA.1(a subtype of Omicron).This platform was also used to analyze some recently collected patient samples from a local outbreak in China,identified by the Centers for Disease Control and Prevention.This innovative CRISPR-empowered SPR platform will further contribute to the fast,sensitive and accurate detection of target nucleic acid sequences with single-base mutations. | Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar AAl-Hartomy Swelm Wageh Abdullah GAl-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang | 2022 | National Science Review2022，9，8： | 3 |
| 18 | Sieving carbons promise practical anodes with extensible low-potential plateaus for sodium batteries显示文摘Non-graphitic carbons are promising anode candidates for sodium-ion batteries,while their variable and complicated microstructure severely limits the rational design of high-energy carbon anodes that could accelerate the commercialization of sodium-ion batteries,as is the case for graphite in lithium-ion batteries.Here,we propose sieving carbons,featuring highly tunable nanopores with tightened pore entrances,as high-energy anodes with extensible and reversible low-potential plateaus(<0.1 V).It is shown that the tightened pore entrance blocks the formation of the solid electrolyte interphase inside the nanopores and enables sodium clustering to produce the plateau.Theoretical and spectroscopic studies also show that creating a larger area of sodiophilic pore surface leads to an almost linearly increased number of sodium clusters,and controlling the pore body diameter guarantees the reversibility of sodium cluster formation,producing a sieving carbon anode with a record-high plateau capacity of 400 mAh g.More excitingly,this approach to preparing sieving carbons has the potential to be scalable for modifying different commercial porous carbons. | Qi Li Xiangsi Liu Ying Tao Jianxing Huang Jun Zhang Chunpeng Yang Yibo Zhang Siwei Zhang Yiran Jia Qiaowei Lin Yuxuan Xiang Jun Cheng Wei Lv Feiyu Kang Yong Yang Quan-Hong Yang | 2022 | National Science Review2022，9，8： | 3 |
| 19 | Total flavonoids from Ganshanbian (Herba Hyperici Attenuati) effect the expression of CaL-α1C and K_(ATP)-Kir6.1 mRNA of the myocardial cell membrane in myocardial ischemia-reperfusion arrhythmia rats显示文摘OBJECTIVE: To observe the impact of total flavonoids from Ganshanbian(Herba Hyperici Attenuati)on the expression of vascular smooth muscle membrane L-type calcium channel alpha1 C subunit(CaL-α1C) and ATP-sensitive K+channel(KATP)-Kir6.1mRNA, and explore the mechanisms of the antiarrhythmic effect of Ganshanbian(Herba Hyperici Attenuati)total flavonoids.METHODS: The treatment group was fed total flavonoids from Ganshanbian(Herba Hyperici Attenuati)for 7 days by gavage with 100 mg·kg﹣1·d﹣1.The blank control group and model control group were given the same amount of normal saline for 7 d.Arrhythmias were induced by performing a myocardial ischemia-reperfusion and electrocardiogram was observed. Reverse transcription-polymerase chain reaction was used to detect the expression of CaL-α1CandKATP-Kir6.1 mRNA in the myocardial cell membrane of all groups of rats. RESULTS: Total flavonoids from Ganshanbian(Herba Hyperici Attenuati) can delay the appearance of myocardial ischemia reperfusion arrhythmias,shorten the duration of myocardial ischemia reperfusion arrhythmias, reduce heart rate, reduce cell membrane expression of CaL-α1C mRNA and enhance the expression of KATP-Kir6.1 mRNAin myocardial ischemia-reperfusion arrhythmic rats.CONCLUSION: Total flavonoids from Ganshanbian(Herba Hyperici Attenuati) can alleviate arrhythmias by affecting the expression of L-type calcium channels and ATP-sensitiveK+ channels. | Yuxuan Ma Yanli Wang Yanyu Gao Yin Fu Ji Li | 2014 | Journal of Traditional Chinese Medicine2014，34，3： | 3 |
| 20 | Synthesis of hierarchical nanohybrid CNT@Ni-PS and its applications in enhancing the tribological,curing and thermal properties of epoxy nanocomposites显示文摘Poor interfacial adhesion and dispersity severely obstruct the continued development of carbon nanotube(CNT)-reinforced epoxy(EP)for potential applications.Herein,hierarchical CNT nanohybrids using nickel phyllosilicate(Ni-PS)as surface decorations(CNT@Ni-PS)were synthesized,and the nanocomposites derived from varied mass fractions of EP and CNT@Ni-PS were prepared.The morphological structures,tribological performances,curing behaviors and thermal properties of EP/CNT@Ni-PS nanocomposites were carefully investigated.Results show that hierarchical CNT nanohybrids with homogeneous dispersion and well-bonded interfacial adhesion in the matrix are successfully obtained,presenting significantly improved thermal and tribological properties.Moreover,analysis on cure kinetics proves the excellent promotion of CNT@Ni-PS on the non-isothermal curing process,lowering the curing energy barrier steadily. | Jinian Yang Yuxuan Xu Chang Su Shibin Nie Zhenyu Li | 2021 | Frontiers of Chemical Science and Engineering2021，15，5： | 3 |
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